LOCUS       DANJJW010000001        89652 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000001 DANJJW010000000
VERSION     DANJJW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(552..2165)
                     /locus_tag="PWS82_000001"
     CDS             complement(552..2165)
                     /locus_tag="PWS82_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0905167.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="PWS82_000002"
     CDS             complement(2306..3214)
                     /locus_tag="PWS82_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905168.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="PWS82_000003"
     CDS             3397..4521
                     /locus_tag="PWS82_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0905169.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="PWS82_000004"
     CDS             4748..5665
                     /locus_tag="PWS82_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0905170.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="PWS82_000005"
     CDS             5658..6491
                     /locus_tag="PWS82_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0905171.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="PWS82_000006"
     CDS             6574..7692
                     /locus_tag="PWS82_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0905172.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="PWS82_000007"
     CDS             7686..9584
                     /locus_tag="PWS82_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL0905173.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="PWS82_000008"
     CDS             9578..10801
                     /locus_tag="PWS82_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0905174.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="PWS82_000009"
     CDS             complement(10887..12821)
                     /locus_tag="PWS82_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0905175.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="PWS82_000010"
     CDS             13047..13703
                     /locus_tag="PWS82_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0905176.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="PWS82_000011"
     CDS             complement(13777..14130)
                     /locus_tag="PWS82_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0905177.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="PWS82_000012"
     CDS             14322..15251
                     /locus_tag="PWS82_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL0905178.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="PWS82_000013"
     CDS             15262..16098
                     /locus_tag="PWS82_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL0905179.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="PWS82_000014"
     CDS             16346..17122
                     /locus_tag="PWS82_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0905180.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="PWS82_000015"
     CDS             17298..18041
                     /locus_tag="PWS82_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905181.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="PWS82_000016"
     CDS             18034..19614
                     /locus_tag="PWS82_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL0905182.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="PWS82_000017"
     CDS             complement(19865..20344)
                     /locus_tag="PWS82_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0905183.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="PWS82_000018"
     CDS             complement(20360..21112)
                     /locus_tag="PWS82_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0905184.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="PWS82_000019"
     tRNA            21229..21299
                     /locus_tag="PWS82_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="PWS82_000020"
     CDS             21660..22385
                     /locus_tag="PWS82_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0905185.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="PWS82_000021"
     CDS             22640..22906
                     /locus_tag="PWS82_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL0905186.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="PWS82_000022"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="PWS82_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL0905187.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="PWS82_000023"
     CDS             23359..23664
                     /locus_tag="PWS82_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL0905188.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="PWS82_000024"
     CDS             complement(23745..24890)
                     /locus_tag="PWS82_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0905189.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="PWS82_000025"
     CDS             complement(24951..25598)
                     /locus_tag="PWS82_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905190.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25791..26084
                     /locus_tag="PWS82_000026"
     CDS             25791..26084
                     /locus_tag="PWS82_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905191.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26128..26439
                     /locus_tag="PWS82_000027"
     CDS             26128..26439
                     /locus_tag="PWS82_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905192.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26436..27060
                     /locus_tag="PWS82_000028"
                     /pseudo
     CDS             26436..27060
                     /locus_tag="PWS82_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27201..27359
                     /locus_tag="PWS82_000029"
     CDS             27201..27359
                     /locus_tag="PWS82_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905193.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27360..27722
                     /locus_tag="PWS82_000030"
     CDS             27360..27722
                     /locus_tag="PWS82_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905194.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27759..28607
                     /locus_tag="PWS82_000031"
     CDS             27759..28607
                     /locus_tag="PWS82_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL0905195.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28597..30072
                     /locus_tag="PWS82_000032"
     CDS             28597..30072
                     /locus_tag="PWS82_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0905196.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30338..30745
                     /locus_tag="PWS82_000033"
     CDS             30338..30745
                     /locus_tag="PWS82_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL0905197.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30748..30963
                     /locus_tag="PWS82_000034"
     CDS             30748..30963
                     /locus_tag="PWS82_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905198.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30967..31347
                     /locus_tag="PWS82_000035"
     CDS             30967..31347
                     /locus_tag="PWS82_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0905199.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31481..31636
                     /locus_tag="PWS82_000036"
     CDS             31481..31636
                     /locus_tag="PWS82_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL0905200.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31705..32178
                     /locus_tag="PWS82_000037"
     CDS             31705..32178
                     /locus_tag="PWS82_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL0905201.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32175..33869
                     /locus_tag="PWS82_000038"
     CDS             32175..33869
                     /locus_tag="PWS82_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0905202.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33835..34020
                     /locus_tag="PWS82_000039"
     CDS             33835..34020
                     /locus_tag="PWS82_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905203.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34024..35199
                     /locus_tag="PWS82_000040"
     CDS             34024..35199
                     /locus_tag="PWS82_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0905204.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35192..36715
                     /locus_tag="PWS82_000041"
     CDS             35192..36715
                     /locus_tag="PWS82_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0905205.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36771..37055
                     /locus_tag="PWS82_000042"
     CDS             36771..37055
                     /locus_tag="PWS82_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0905206.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37042..37377
                     /locus_tag="PWS82_000043"
     CDS             37042..37377
                     /locus_tag="PWS82_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0905207.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37672..38298
                     /locus_tag="PWS82_000044"
     CDS             37672..38298
                     /locus_tag="PWS82_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905208.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39040..39877
                     /locus_tag="PWS82_000045"
                     /pseudo
     CDS             39040..39877
                     /locus_tag="PWS82_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39942..40376
                     /locus_tag="PWS82_000046"
     CDS             39942..40376
                     /locus_tag="PWS82_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905209.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40444..41766)
                     /locus_tag="PWS82_000047"
     CDS             complement(40444..41766)
                     /locus_tag="PWS82_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL0905210.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41920..43560)
                     /locus_tag="PWS82_000048"
     CDS             complement(41920..43560)
                     /locus_tag="PWS82_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL0905211.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43890..44360
                     /locus_tag="PWS82_000049"
     CDS             43890..44360
                     /locus_tag="PWS82_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL0905212.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44989..46092
                     /locus_tag="PWS82_000050"
     CDS             44989..46092
                     /locus_tag="PWS82_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0905213.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46376..47056
                     /locus_tag="PWS82_000051"
     CDS             46376..47056
                     /locus_tag="PWS82_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0905214.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47146..48180
                     /locus_tag="PWS82_000052"
     CDS             47146..48180
                     /locus_tag="PWS82_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0905215.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48194..48529
                     /locus_tag="PWS82_000053"
     CDS             48194..48529
                     /locus_tag="PWS82_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905216.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48513..50993
                     /locus_tag="PWS82_000054"
     CDS             48513..50993
                     /locus_tag="PWS82_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905217.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51049..52608
                     /locus_tag="PWS82_000055"
     CDS             51049..52608
                     /locus_tag="PWS82_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905218.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52605..53969
                     /locus_tag="PWS82_000056"
     CDS             52605..53969
                     /locus_tag="PWS82_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0905219.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54140..55210
                     /locus_tag="PWS82_000057"
     CDS             54140..55210
                     /locus_tag="PWS82_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905220.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55225..55893
                     /locus_tag="PWS82_000058"
     CDS             55225..55893
                     /locus_tag="PWS82_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905221.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55913..56491
                     /locus_tag="PWS82_000059"
     CDS             55913..56491
                     /locus_tag="PWS82_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0905222.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56894..57805
                     /locus_tag="PWS82_000060"
     CDS             56894..57805
                     /locus_tag="PWS82_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0905223.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58061..58978)
                     /locus_tag="PWS82_000061"
     CDS             complement(58061..58978)
                     /locus_tag="PWS82_000061"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL0905224.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59180..59617
                     /gene="zapA"
                     /locus_tag="PWS82_000062"
     CDS             59180..59617
                     /gene="zapA"
                     /locus_tag="PWS82_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL0905225.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59675..60222
                     /locus_tag="PWS82_000063"
                     /pseudo
     CDS             59675..60222
                     /locus_tag="PWS82_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60337..62697
                     /locus_tag="PWS82_000064"
     CDS             60337..62697
                     /locus_tag="PWS82_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0905226.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62784..63098
                     /gene="trxA"
                     /locus_tag="PWS82_000065"
     CDS             62784..63098
                     /gene="trxA"
                     /locus_tag="PWS82_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL0905227.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63280..65058
                     /gene="uvrC"
                     /locus_tag="PWS82_000066"
     CDS             63280..65058
                     /gene="uvrC"
                     /locus_tag="PWS82_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL0905228.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65203..66102
                     /locus_tag="PWS82_000067"
     CDS             65203..66102
                     /locus_tag="PWS82_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905229.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66095..67357
                     /locus_tag="PWS82_000068"
     CDS             66095..67357
                     /locus_tag="PWS82_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905230.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67520..69418
                     /locus_tag="PWS82_000069"
     CDS             67520..69418
                     /locus_tag="PWS82_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL0905231.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69717..70436)
                     /locus_tag="PWS82_000070"
     CDS             complement(69717..70436)
                     /locus_tag="PWS82_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL0905232.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70773..71105
                     /locus_tag="PWS82_000071"
     CDS             70773..71105
                     /locus_tag="PWS82_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905233.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71092..73095
                     /locus_tag="PWS82_000072"
     CDS             71092..73095
                     /locus_tag="PWS82_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL0905234.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73114..73584
                     /locus_tag="PWS82_000073"
     CDS             73114..73584
                     /locus_tag="PWS82_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL0905235.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73605..74189
                     /locus_tag="PWS82_000074"
     CDS             73605..74189
                     /locus_tag="PWS82_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL0905236.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74204..75190
                     /locus_tag="PWS82_000075"
     CDS             74204..75190
                     /locus_tag="PWS82_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL0905237.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75180..75491
                     /locus_tag="PWS82_000076"
     CDS             75180..75491
                     /locus_tag="PWS82_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL0905238.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75513..77294
                     /locus_tag="PWS82_000077"
     CDS             75513..77294
                     /locus_tag="PWS82_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL0905239.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77287..78663
                     /locus_tag="PWS82_000078"
     CDS             77287..78663
                     /locus_tag="PWS82_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL0905240.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78670..79305
                     /locus_tag="PWS82_000079"
     CDS             78670..79305
                     /locus_tag="PWS82_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL0905241.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79335..80690
                     /locus_tag="PWS82_000080"
     CDS             79335..80690
                     /locus_tag="PWS82_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL0905242.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80882..82276)
                     /locus_tag="PWS82_000081"
     CDS             complement(80882..82276)
                     /locus_tag="PWS82_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0905243.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82368..83339
                     /locus_tag="PWS82_000082"
     CDS             82368..83339
                     /locus_tag="PWS82_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL0905244.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83336..84118
                     /locus_tag="PWS82_000083"
     CDS             83336..84118
                     /locus_tag="PWS82_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL0905245.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84287..84658)
                     /locus_tag="PWS82_000084"
     CDS             complement(84287..84658)
                     /locus_tag="PWS82_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0905246.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84792..85235
                     /locus_tag="PWS82_000085"
     CDS             84792..85235
                     /locus_tag="PWS82_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL0905247.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85278..86087)
                     /gene="yidA"
                     /locus_tag="PWS82_000086"
     CDS             complement(85278..86087)
                     /gene="yidA"
                     /locus_tag="PWS82_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL0905248.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86106..87473)
                     /locus_tag="PWS82_000087"
     CDS             complement(86106..87473)
                     /locus_tag="PWS82_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0905249.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87457..88287)
                     /locus_tag="PWS82_000088"
     CDS             complement(87457..88287)
                     /locus_tag="PWS82_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL0905250.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88450..88887
                     /locus_tag="PWS82_000089"
     CDS             88450..88887
                     /locus_tag="PWS82_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL0905251.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88963..89583
                     /locus_tag="PWS82_000090"
     CDS             88963..89583
                     /locus_tag="PWS82_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL0905252.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18470 g        26460 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag ggggataaat atggaattaa
    25801 aaattgacaa actcacgctt gatgatccag cttttaaaat tattgacgat cgagttgttc
    25861 aacaagtaga gcaaactcaa ggaacgttac tttcattatt agaaaaaaag taccagtttc
    25921 caaaatatat gaacaaggca caagctgcta agtatatgaa cgtttcatac aatactatga
    25981 tgcagaaata tgttcctaac ggtttaaagt tgattatagt tgatggagtc gtaagaattt
    26041 cacaagatga atgtgatcgc tttatggaag aataccaaaa ataaacgcaa gccgtggggg
    26101 caaagggatt aataggagga aataataatg gaatcgacac aaaataaagc aatcgaaaaa
    26161 gtattatcaa caattcttac agaagacaca gcggcagaat tagcaaactt ggaaggcaaa
    26221 gcgctagaag aaacgtttga atgtttgtac gaacaaatgg actatcaaaa attgctacca
    26281 caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg atttagtaca agcagagttt
    26341 aaagaacgac tttctattga agagtatcaa gaaattttgt atcagcaagt tgagttgtta
    26401 gcgagcttgc tagggcttga actggaggac gttgaatgaa aactgaaatt tggaacggac
    26461 acattataag atttgtcgat attaatgatg aatggtgggc agtggcgaaa gatgttgcgg
    26521 aagcgttagg tcttaaacag gttactagag ccattcactc tttgcctaaa gatggggtta
    26581 ctactagtaa ggtcatcgat tcattgggca gaacacaaga tgtaaacatc atcaacgaaa
    26641 aaaaatattt accgcatggc attcaaaagc cgtaaaaaag aagccgaggc atttcaagac
    26701 tggattttcg acatcatcaa agaactacgc cagtccacag gacttgaagg ctttcaagta
    26761 ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga ctaaattaag ccacgctatt
    26821 actgaaccaa aaccagtaga ctacattaaa gcgaatgtga tagccaataa agctgtatca
    26881 actatttatg gtcattccaa aatggtaaaa aagaaagata tgactcctga aatgttggtt
    26941 gatcgtgaac ctattttaga tgagacagta gaattaatga ctgttaaaga aaaatatggg
    27001 ttgcagtttt cagtgagtga gaaaatttat aatcgttctg ctgaattgca gacaacttag
    27061 gagcattaac atgatcgaga acaaagaaaa agccttatcc ataacagtct tggcggacag
    27121 gataaaggca acttaattgg gataaaaccc gatattcttt gttccgatta taccatatta
    27181 aaaaatcgga gggaaccatt atgaaacgaa acttaattat aggaagcatt ttaatcggcg
    27241 gaattttcgg cttattacca ttaattgcaa aaatagctat ctttgcagga ttagcgttat
    27301 atctcttcac tgcttatgac gagtacgact atcaattaag aactggaggg cataagtaga
    27361 tgtacaaaac aaaactatta aaccaactcg atagccttga attagaggaa attaatcaag
    27421 ggatcgctga actagaaaat aatatcggga aaacctactt tggaaattca ttcaatgaaa
    27481 aactaacagt tttgtatgta ctcaaaaaac atgcagaaca caaaataatt tgtcgagaaa
    27541 tcaacgagct aaaaaatcag attttaaccg catggttaaa cattactgat atgcaagaag
    27601 caagggtgaa aacatttaat acatgggtaa agtatcagaa tcaattaaaa ggtgctgaat
    27661 ttgttcggga tgggttgaaa tacgaacttg agcagttgaa gctcatggag gtgtctgaat
    27721 gatttatacc agtattttga aaaaggagga gaggtttatt ggcaaataat tatcaagcag
    27781 caacggaatt acaaaagtcg ggatggtcaa tttatccctt atctcctgga actaaaattc
    27841 caataaaagg ctctagtggt tggaatgatg caactaacga ttctaatcaa attaatgaat
    27901 ggtggacgga agatgcaact agaaatattg ggttaatgct tgaagatcat caaatggtgg
    27961 tagttgacct agatcaacat tctgaaacac gaaacggcgt tgagaattat aaaaaacttg
    28021 cttcaatgta tgatccattt ccttctactt atacggaaat cacaccaggt aaaggaatcc
    28081 atttcttctt taaaattcct gatggtgtaa ttatccaaca agaaacaggt gcatttgctg
    28141 atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg gattgatatt atcactaaag
    28201 gagttccaat agcgccaact atgattattt caagaagtgt tgggagagca tatgaaccta
    28261 ttgaaaacgt atctctaaat aatattgcac cagctcccga ttggctagtg aacctactgc
    28321 taaaaaaacc gcaagaaata aagactcaat accgacccgt agcaaaaaca gggacagcaa
    28381 aaaaattaga tatgattgtt caaggcgctg gcgaaggtag tagaaacgat catttaacta
    28441 agttatgcgg ttggttacta tggcacggtg tagataatca aactttagca gaattgattt
    28501 atacagcaaa tgcgtacaat gaaccaccgc taaaagataa agaagtgaat caaattattc
    28561 gatcgatgat taaaaaagat ttgaggggga aagctcatgg ctcataagca taaattagat
    28621 agtattttag actttcccga agccagcgag cgtgaagaca atatcattga attgaaaacc
    28681 tggatgtcac gattaagatg caataaagac gatcagatta aaagtaacag tgttgtgaat
    28741 gcagaattga ttttaaccaa tgatagcaat ttagctggaa caattgctta taacgaattt
    28801 agcggataca ttcacctatt aaaagattct ccttggatca atcgtaccgc tggagaatgg
    28861 gaagacagtt ttgaggatgc cttaacggca tatattgaag aaaactacaa tgtagttttt
    28921 gatgacaaca aaatacacaa agcagtagta aatgtggcta gaaagaatgt tttcaaccca
    28981 gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc ctagactgga aacactgttt
    29041 attgaccttt taggggtaga cgacaacctt tacacacgag aagtaacaaa acgttggata
    29101 gtcggatcag tcgcacgtat ctataaacca ggaataaagt ttgagatcgt tccagttttg
    29161 gatggaccgc aagggattgg taagagtacc gttccagcat tactttacac ggatgatttt
    29221 ttcaccgatt cattagattc gctaggtgaa aagaaagatg attatatgca gcttcaaggc
    29281 aatgtaatta ttgaacttgg agaattatct tcaatgaaca aaactaaaat tgaacagata
    29341 aaaaacttca tcagtgccaa gatcgataaa attagacctc cttatggtcg gaatgtcata
    29401 gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg atgggcagta tttgaaagat
    29461 gatacaggaa accgccgttt ttatcctttg ccttgtaaga ataagccaaa gatgaatccg
    29521 tttaaaacaa aggatgaata ttttcttcaa gtgttagctg aagcaaaggt gctttttgat
    29581 aaaggacaac gaatctattt tagtcccgat gaagataaag aagtcttaga aattgcaaaa
    29641 gactatcaag aggatgccaa gatggagaat ccaattaaag aagcaatcac taaatatttg
    29701 gaaatggaaa tcccgtataa atgggaagaa gctccagcgt gggcgagacg ttcttattat
    29761 cagaactatc ctgacagtaa atgtaatgag aaaaatttac agcatttcta taatggagct
    29821 actatgcaac aagagtttta tttagttgat agcgtactca ccgctgatat tttggaagct
    29881 gtttttgata aacaagcaaa ggatttattg aatggtcgat cagatgcaga aactaaaaag
    29941 attgccttga ttattagcag tattcctggt tgggaacgga agcaactgcc aaaaagaaat
    30001 aaacgacgag gattttacaa taagtcaaat gcctatgaaa ataaaaatcg agctggaaag
    30061 tacaaaaagt aacgggtagt aacgggtcag taacacctag cccgttacta gtttaccctt
    30121 actgccccaa gggatacagc aatttggtaa cggggtaaca ccttatttcc ttaaaacttt
    30181 tgaaatagtt agctaatatg ctggggaata atgttactgg attttacccg ttacctgtta
    30241 ccaaaacgct agaacccttg tccccgttgg gattgagtgg taacgggtac ctgttacgta
    30301 ggtgttacca cgtgttacga atcaaggagg aaagtaaatg ctcaatatta tttcaacaaa
    30361 caaagcaccg aactttcaat atacagatga aatggatcgg tttctaatga acacgctggc
    30421 atttagtgtg ggattggtaa cagaagatta ttccacattt gatccagaag tattaaagat
    30481 aatggaagaa gaacctgatt ggctgcagga gtcagtagcg tggtgtcaat cgctagtagt
    30541 gggatcattg gtagatagtg gcaactatga tgatacaggt gagctaatgg atgaatttaa
    30601 ttgtctgctt aacctatatg atcgagcgag acaacgagag cttacatcga atgaggataa
    30661 tttgttttta aacatccatg ataagttctt ggctttgcta ctaacagatg atgaattgat
    30721 aactaattta ttggaggtag aataattatg catatggaaa gtattagata cttcgatggt
    30781 gagaaacaca ttgagtattc aaacactcaa gaagatgtag actttattag ttttgaggaa
    30841 gataaaatca ctaaagttaa ttttaaagac ggaacgtgct tgaagatagt ttcgccttat
    30901 attgaataca aatcaaaatg ggaacaagat gattctgacg aacgagatcg ctttggttgg
    30961 taatctatgg cagttaaaaa acaatgtaat catgctggat gtaaaacatt gattgattat
    31021 aggcagaagt attgtgggaa gcataaagca aagcaaacgg caattaagcg tgaagaaaga
    31081 aagcaaagcg aaggtaaata ttttcaattc tatcaaagta gaacatggcg aaaggcttca
    31141 tacttgtatc ggttaaatca tccagtatgt gaggattgcc tagaagaagg cttgataagg
    31201 aaagctgatg ttgtagatca caaaattgaa ctaaaagatg attggaatag acggttagat
    31261 gaaactaatt ttcgttcttt atgccatgca caccataatt ccaagacagc caatgaaaga
    31321 caaagacgag aaaagagtac cctttgagtt aagggggcat agtgtgaaac ttactgacaa
    31381 tcgatgccta ctcattttgg tacaaataac cgttgtgaaa aggcataagg gtaattacaa
    31441 ataacgatta cacttgtaat cacaagtgaa aaagtatata atgaaagtag gagatttatt
    31501 gaaaaataca attgttatta ctgataaaac agggaaacag cgcaccattg agttacctca
    31561 ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc atttatatag ataaattaga
    31621 taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct taattgctta cgttcctttt
    31681 tcttttgtct gaaaggagga caacatggga caccctaaaa tattacacga tacaaaaggc
    31741 aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta gagaagaatt gttcaagcag
    31801 caaccattaa taaatatcac gccccctgac tggatggcag cgagcgctag aagtgaatgg
    31861 aatcgtatcg taccaacatt aaaaaaagat tatccattga gtgaagcaga ttatggttca
    31921 ttggtagcct attgtttagc ctttgctcga atgaaaacag ccgaagccga gataagaaaa
    31981 tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag ccaatccagc ggttcgggtt
    32041 caatcacaag ctatgagtga tttgaaaaaa caagccacct cactaggtat gaccttagaa
    32101 tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg aacccgaaga cccgttcaaa
    32161 gagttgatga attcatgaat gattacattg agaaagtcct atcaggtaag ctgattgcac
    32221 ccaagaaaat tatccaagcg tgtgagcgtc atataagcga tttggagcgt tctaagtcag
    32281 atagcttccc ttatgtgttc gatgaagaac aagccaccaa agcgattaaa ttcattgagt
    32341 tgctaccatc tacggacggt aaagcaatca agatgttagg atttcaaaaa ttcattctag
    32401 gcagtcttta tggctggcgt actaaagaag ggaattacag gcgattcaat cgagcgttta
    32461 ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag tggcatggct gccaatgcgt
    32521 tgattatgga acaagaacca gcagaagcaa ggcaagtatt gtttgtaagt aacgctttga
    32581 aacaagctaa attgggctat gatatgctgt ctaattcact tagaaacgtg gtcaagtcta
    32641 gtaagttttt aagaccacaa ctgaaaatta tgaactctaa gattcagcac ttgccctcta
    32701 attcgttcgc tatggcactg gctagtgaaa ccagcacgct agatgggttt gcaccaacaa
    32761 ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa aacgtacaac gtcattaagt
    32821 caggaatgac ccaacaaaag aacggcttat tgtgtgttat tagtaccgct gggcttgatt
    32881 taaacgttcc tatgtacgag gaatacttat tgttagatcg tgtgctaaaa ggcgaagaac
    32941 aagccgacag gtactttata gcgatatggg aattggacga tcccgaagaa attcacgatc
    33001 aagagaaatg gatcaaagcc aatccgattt ttgaaagtga agaaatcaaa aaagtaatga
    33061 ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca aaataacctt aattctgtat
    33121 tggtgaaaaa cttcaattta tggagacaag cgagtgagga cagctatatg attgctgaag
    33181 actggcaggc aaccgaagta gaaccacaag atattacagg caagcccgtt tatatcggag
    33241 tggatttatc taaaacagat gatttaacta gcgtttcatg gatcgtaccg ctagataacg
    33301 gcgaacttta ttgtgattca catagctttg tagccaccaa atatgggctt caagacaaag
    33361 aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc tggtgagtgt tccattactc
    33421 aattagaaag tggaatcgtg gactatgacc aagtatttca gtttattcaa gatttgattc
    33481 aagaaaatga tttggaatgt atggggattt gttatgaccc gtataacgct aattcgctta
    33541 tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt aagacaagga acgattactc
    33601 taaacgttcc gacccgaact tttagagaac aagtttatga aggcaacgtt attcacaata
    33661 aaaatacgat tctcacccat gcagtgaaca acgctatttt aaaaacggat aacaacggca
    33721 ttcagattaa taaatcaaag aacagtaaca aaattgatcc aatagctgca ttaatcaatg
    33781 cctatgtgtt tgcaatggat tacttcacca caacggaagg agcgaaagca gacaatgaat
    33841 tttatacaag tgaagaattt tctttctaat tacattcata ccgttctttt acttcttgga
    33901 ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg tctactatgg cttgttagcg
    33961 ctgggcatag tgcttattgg ggtagcggtc atgctaaata cagaacagaa gggaggttaa
    34021 aagatggcat tttttaaagc gagacaaaat acaacgggag atcctttcct acaacatgtg
    34081 gtatcaatcc aatcggatga ttacaccacc agctttacaa gcgttcgtgc attaagaaat
    34141 agtgatgtgt ttgcagccgt tcgaattatt gctagtgata ttgcttcaag tccgattcaa
    34201 ttggtcaaaa acaatatgcc acaagctgat aatgaactgg tgaagttact aaacgagaaa
    34261 cctaattcag aaatggatgg ttggcattta aagtttgctt tggcggtcaa tatgttgcta
    34321 aacggtaata gctttgcaga gattaaacgc aacggtgaaa aggtagaaga aattcactta
    34381 ttacctaact caagtgtaac ggttactcaa ttagataatg gtacgttgtc ttatcagatt
    34441 ggtgataaaa aaagacgtgt gaagtctagc gatattttgc acttcaaata tttcacccaa
    34501 gatggtttaa caggattacc accactttat gcgttacgtg atgaactgaa aattcaacaa
    34561 gctggcaacc gcacattgca caatttcttt actcgtggtg tcagtgggtc aggtattttg
    34621 aaggttcata agtcagattt agacggatca gcaaagaacg caattcgtga aaagtttgaa
    34681 gaagccaacg gttcaagtag tggagataat gctcttagaa cgatcattct tgatgaaaca
    34741 atggactaca aaacattaga aataaacacc gatgttttga agcttatcaa ttccaatgat
    34801 tggaacacaa aacaaattgc caaggcgttt ggtgtaccga ttgagcgttt aggcgttgaa
    34861 aatgaacatt ctagcaccgt tcaaagtaat ctccagtata tccaaagtac gctgattcat
    34921 tactttaatg tgtttgtgag tgaattagat acgaaacttg aaaccaatat ccgctttaat
    34981 tccgatcagt tactagagac tgatcctgaa acaaaagtaa aaaatgtatt ggatcaggtc
    35041 aaagggtcac ttctcacgat taatgagggg cggtcgaaaa tggggctacc ccccatggat
    35101 ggtggcgatc gtttactagc aagtttgaac tttacgtatt tagatacgtt ggagaaatat
    35161 caattaaaag aacaggaagg agttacacca gttgaataat gaagaagaaa aggaaaaacg
    35221 gctgacagaa gaagctgagc taacagccga ttctcccaaa gtagagaaag aaaatgaaaa
    35281 acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg aaattcgggc aaccgtcaaa
    35341 agatttaggc ggctttgtag aagtgattac acccgaagca ttaaaagagg tggatttatc
    35401 aaatgtgttc ttattatatg gacacgatta ttcaaagcca ctagctagtg tcaaagcagg
    35461 tacgttgaaa ttgaatgtcg atgatacagg attacatttt gaagcagagc taaccgacac
    35521 gacctattca aatgatgtgt atgagaatat ctcaaaaggg gttgttgatt ctatgtcctt
    35581 tgggttcgtg cttggattag attcattcga caaaaaagaa gatggcacaa ttgaacgatc
    35641 aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg accgttcccg cttatgattc
    35701 atcaaatgtc caagtaaata agcgttctta cgaatcgttt atgagtaaca accaagcaaa
    35761 acaaacaaac aatagcttag aatccacttc taaagcacaa aaggagagta ataacatgga
    35821 aaaaacttta atcgataacg aaaaaacaga attgcgtggg tatgaagaat atatccgttc
    35881 acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca gcggcagttg ttcccgaaga
    35941 agtaatcggt gaagtctttg atttgaaacg ttcaaattat aacttagctc aatatgcaac
    36001 agtaaaaaca gtatcaaatg gacaaggtaa atatccagta gcaactaacc aacaagcagt
    36061 gttagcaaca aaagctgaac ttgctgaaat tggtgatatt gacgctgaaa tgtttacttc
    36121 agttgattat aaagtagaaa ctcgtgctgg taagattgcc ttatcaaatg aggttgtgga
    36181 agattcagca gtgaatattg tacaagaggt caaagatcag ttagcgaaat tggtagaaaa
    36241 cactgacaat aagcatatca tggatttatt aaaaacattc actaagaaaa cggctgctac
    36301 gttggacgat ttgaaacaac tatacaatgt ggcattagac ccagcattaa ataaaatggt
    36361 aattctaaac caaagcggat attaccacct agatacattg aaagattcag atggacgtta
    36421 cattttacaa cccgatgtga cagcacctag tggtaaatca ttattcggta tgccagtagt
    36481 attgattgca gatacattgt ttgccaatcc taaagctgga acattcccta tgattatggg
    36541 ggatattgca caatctatct ttgttgctcg tagaaatcaa gtaacgactc aatgggaaaa
    36601 attcgattac tactcacaag gacttgcagt gatcgttcgc aacgattata agaaaattga
    36661 tcaaaatgct tcagtgtata ttgagtttac gccagttgta acaccaaaag tatagaaaaa
    36721 tattgggcgg cggtttatcc cactgccttt ttttcattag gagagataaa atggtaattt
    36781 tggatagcat aaaaaaaagt atgcggatcg atcacactat tgatgatgac tttattcaac
    36841 aattgattga tacagcaggc gaatatataa aaagtgctat tgatagtagc gcaacggata
    36901 aagatatgga taattatcag caatttgatt tggcggtgtc attacttact caacattggt
    36961 atttgaaccg tcaagaagcc agcagcgaac ggataccagt aacggtacaa gcattagtac
    37021 aacaaatgag aggtgcttat tatgccaatc attaagaatg taaatgaatt gactgagaga
    37081 gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg ggcagatggt ggatagtgaa
    37141 gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa tgctaaaaga cgtgcttgct
    37201 agtgtaggta ccattcttga aggaacgttg acgtttatta ttcgatacga tcaagattat
    37261 gaactaacaa acgatatgaa agtagcttgg aaaaacaaga gttataaaat tatttcaatc
    37321 aatgaaggaa cggcgtttaa agattataca acgatcatag ctaaacagat ttcttagaat
    37381 gattacactt gtaaacgttg tgggtattta gtataattaa ggtagtaaat gaagggttag
    37441 ctgcctgaat tttgcaagac ttagctagtc gaaatctatt gttggactga aaattgtagg
    37501 tgtggttgca aacatgctgg actaagaaag atgatagatg atgaacgttc aattttaata
    37561 caggcatgct aggactgatt ggatggtttt ggcaagctga ttgtgtagaa atcaaagaac
    37621 acgtctgttg tggacgtgtt ttttttgtta taattcaaaa gaggtgattt aatggacaaa
    37681 gcagagttta caaattttta ttggaagtat tatctacatt tagaaaatga gtttattaat
    37741 accacagatt ttgtgatgct cgatgagaat aatttcaaaa cattctctat tgaatatcaa
    37801 aaactactac tagcgattgg ttcggagtgt gagattattt tcaaagaact ttgcggattt
    37861 gatagcagta gcaataaaag cataactgat tttaaagctg ttattaaatc tagcgaatta
    37921 atgtgtttgg ataatggtgt tagggttatg aattctttaa cattgacttc actaaaacct
    37981 tttggagatg ggtggcctga agatacacca gattggtgga aaatatataa cagagtaaaa
    38041 catggtcgtt cttcaaatta taaggaagca aatttggaaa atgtgttgta tgctttagca
    38101 agtctatatt tattagagga atatttgcat aagaaagtaa tactagaagg tgaagtagac
    38161 tttataagtc cagagtcaaa tctatttacg ttatcttggg aaagaaaaca ttcgtcgatg
    38221 caaaaagtga cgtttgaaaa agtggattca aattatgtag ctccactcat tgattttaaa
    38281 gaacaagaag aaatttaaaa acccccacgg cttattgaag tttttgtatg ccatattgta
    38341 tgccatttgg tttaaatcgc ttgaaaaata gtgatagata actctaattc atttagtcat
    38401 aaacgctgtt ttgataacct ttatagttct ttgatatggg tttctcattc atggcggaag
    38461 aagaagaata gaacaaaaga atgaattaat aagatataaa aaaagggatt gtgacaaacg
    38521 tcttgtcaca atcccttttt ccaaataaac gatgttcaat cagctgcccc tcggcattcg
    38581 ctcgaaaaac ggcgaaatat gaagaactat tttgccattt gtttctctaa taatcaggtc
    38641 aaaacgttga cttcacagtc tcttccttaa acggtgttct cctgccaact cctcggtcac
    38701 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    38761 agctgaacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
    38821 taataagttc aaccaaacga aaattatgag gagctatttt tgtttggctg ttcttattac
    38881 ttgatgcagg acagcttttt cacaacctct tttcgtttag cactaaattt ccttaaaaag
    38941 tagaaaagct ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac
    39001 aatcgctaaa agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt
    39061 gtggtgatcg ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg
    39121 atcaaaccga ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca
    39181 acagttgcgg tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa
    39241 tcactgaatt ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc
    39301 ggcaacattg tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg
    39361 atccaaattt ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat
    39421 cagccaaaat ttcatttgac gtcatggact tggtacctta tatgtggggg gggttctggg
    39481 ctttttagct agttttttag gtattggtgg tggaccagtc aatgtttctt tgttgatgct
    39541 gatgtttgca ttgccaataa aagaagcaac actatattct ctatctacga ttttcttttc
    39601 tcaactggca aaattggtaa cgattgcttt aacaagtagc tttatgcgct ttgatttatc
    39661 tatgttgttt tatgttatcc ccgctgctgt tattggaggt ttgtggggag caagatttag
    39721 cagggttttg tctccaaaaa aagtgacctt tatttttcaa gtaattgtcc tcgtggttct
    39781 tttggtcaat tgttacaatg cctttgtcat cttacaaaca ttaggagaat ctagactaac
    39841 gcaagtcaaa atcactgaaa catattgttc taaatgaaaa aaagagtata ctatgataaa
    39901 aaaattcatt ttaaaaaatc agaaattggc tggtgatttg gatgttacgg gaaatgcgtt
    39961 tagaagatgc agaagcacta cgtgaaatca atgcggatca attagggtat gatataccat
    40021 taaccgttac ggtgagacaa atgaagaaac tgctgaagga gccagagaaa aattttttct
    40081 tggtttatga agatcctact tctaaaaaag cagctggcta tgtacaagcg gagtcctatg
    40141 aatcgatttt tagcgatcca atgttcaata ttatggcact tgctgtgtct aaatatgctg
    40201 aaaaaagagg catcggaaag gctttgatga ctggcgtaga agaagaagcg aaaaaacgag
    40261 gaataacgac aataaggctt aattctgcag aatacagagt agaagcacac caattttatg
    40321 aacacatcgg ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc ctgtaaatct
    40381 ttatcaaaaa aatacccgat caccaagccg atacttgatg atcaggtatt tttgtttttt
    40441 tgactaagaa agtaattgca tcaagaaacc agagatgatc aaaacgatgg caccaccaag
    40501 tcgattgccc atttgagcaa aagcaataag ttccatccga ttagctgcag ataagacggc
    40561 tacatttcct gttccgccca tcgagttgtt gcataagcca gcagtaatag ttgattcaat
    40621 tggatataaa ccaaacaaac gaccgacaaa tcctgaaaca actgaaatcg cgataacact
    40681 tgtcagacat aagacaacaa attgccatgt caaagcagaa gctaatacat tcagattcag
    40741 caacgcaata ccgataccgg caagaacagc tgctgtcaag tttttgacaa tcaagttatt
    40801 gaacatgata gctgcatctt cataatactc tggtaatagg ttggcgattt tacagatgac
    40861 aacgataata atcataaatg cgtaagcatg gactttagga acaaaatagt taccaatttg
    40921 tcctaagatg aagaaagtca atgataccat caaaccaaca cctaactgtg gaatggaagg
    40981 tttcacagtt cgaggttttc catcgccaag atcgcctttt tccattaatt taccattgcc
    41041 gttgaatttt ggcattgatt gtccagcacg agcaactaat gctgcaccga tgatagctaa
    41101 gacatttgtc attgctgtcg caggaatcag gcgggaaatg atagaagaag catccgtacc
    41161 taaaacattt gcataaatcg ttgataaagg aacggctccg gctccaacac caccagccat
    41221 cgcaggtaat gagatgtata aaacggaatt tgcaaaacca tttccgataa gcatgcccac
    41281 gagtcctacg gcaaaaaagg agacgaccat tgaaataagt gcaacgggaa taaaacgaac
    41341 agaagctttc atcaatagat tacggttcat accaaggatg ctaccagtaa tcaaggcagc
    41401 aatataaaag tcccaaaagc ccatattgct gacaaagttt tctgtggatt tcactacata
    41461 ttcaggtaag atcccaaatg tagcaatcgc agcagaagcg aagatcgcaa aaacggaacc
    41521 accacccaaa taacttttga cgatagggat ttttgtcccg ataaaatgaa ataaattacc
    41581 gaaaataacc aaaactgcga ttggtccaat catattcgtc ggtaatttcc ctaaagccat
    41641 tgcaatgatc aggacaagga ccatgatcaa ataaagtggc aaacttacgc cggtgatttt
    41701 tgtggaccaa aaaccttctt ttttcttttc ctcaactgca gttgcagttg ttgttttttg
    41761 tgccattttt acagatcctt tcttattctg tatcatatga aaaaagccaa agaaaagcga
    41821 caaaagagca ggagttggct ggccgctgtt ttgccgcagt cgcttttttc tttttagacg
    41881 tcatacaaag tttctgtatg ttcttttact ataacgatct taatcttcta aagatttata
    41941 ttcaggaacc catttagcag attcaactgc ttctttgata tccgtgattt cttcacgatt
    42001 caagttttgt gcgacaactg attttgcgac agtttcagca atcgtttgag aaaattcagt
    42061 gatttttgct actggaggca agatagctgc tcctggtttt gttacatcaa cgattccgcc
    42121 taatgctcga ctagcttgag aaatgatttc agcatttact cgtgtagctg ttgaagcaat
    42181 caaaccaagt cctaaacctg ggtacatcaa agcattattt gcttgaccga tttggtaagt
    42241 tacaccatta tattcaacat cagcagcagg gatacctgtt ccaactaatg cttttccgtc
    42301 cgtccattct attaaatctt ttgcttttgc ttctgccaat tttgttggat tagacaaagg
    42361 catgatgatc ggacgtggtg tatgagcagc catttctttc acgatttctt cagtgaatgc
    42421 tccaggttgg gtagaagtac caatcatgat tgtcggatgg atcgctttta caactgcttc
    42481 taagtttgtc aattcatcag cattagtgaa atctgagcgt ttgcgagcga atgggatttg
    42541 accaggagtc aatccttctg tatcttcaaa caataaacct tgtttatcta cttgatagaa
    42601 atgttggcgt gcttcttctt caggtacacc ttgacggatc atctcatcta aaaggatgtt
    42661 tgcgataccc acaccagcag taccagcacc aaatgtcaaa attatttgat ctttcaattg
    42721 ttcaccggat atattcaatc cgcctaatac acctgctagg acaacaattc ctgtaccttg
    42781 gatatcgtcg ttgaaagtag tgatttgatc ttcgtatttt tctaagatcg ctgcagcatt
    42841 tcctcgtcca aaatcttccc aatgcaataa caattctggg aataattcgg tagctgattg
    42901 aacgaattga tcgatgaatt catagtaagt ttcgccttct acacgagcat gacgattgcc
    42961 taagtataag ggattattca ataattcttc attattagta ccagcgtcga tcgatacagg
    43021 caaaacttgt gctggattaa taccagcagc agcagtatag accatcaatt tgccgatagc
    43081 gatgtcaaca ccgttcacac cccagtcgcc catacctaag attccttctg catcagtcac
    43141 aacaatcaaa cgaatgtctc gtccatctgc cgcgttcttc aaacttgctt tgatgttttc
    43201 tggttcgtcg attgaaagaa atgcagcatc ttgcggtttc aagaaaattt cgttgtattg
    43261 ttcgattgaa tccgcaacga caggatcata aacgacaggc atgaattcaa ctaaatgttc
    43321 gcccatcaat ttataaaaca atgtccgatt cgtattgaat agattcatta agaagatacg
    43381 tttttccaaa tctgtttgtt tgcttaagta ttgaccataa gcttgaacag attgttgttc
    43441 cagtgtttgg acagtacttg gaagcatacc agtgattcca tattttgccc gttcttcttt
    43501 tgtaaaagct gttcctttat ttagaaaagg attatttaat aagtttaacc ctgtaagcat
    43561 gaatgattcc tcctaagttt taatacaaac gaagtataga agtcagatga tgatatagtc
    43621 aaaaagagca ggtatgataa attttattta tatgtaatat agccaaaaga aatcaaaata
    43681 gcagtatttg ttctttttat atcgacaatc cttgcttgaa tactatgcgt atctcttata
    43741 tgctagagaa agaaaagtag aagaataagg gatagatgga atggataaat aggatttctg
    43801 aggaaaaaag cacatgatag cgttgatagg agtcacctat gtcaggtgat aaaaatacat
    43861 aagtagaaaa ttaagaggag gaagagaaaa tgaaagaagt tcaattgatt agaaaaagcg
    43921 aactatcaga aggcggttgc aatgcatgtg gcgtagtaga agcgacaagc tatacattaa
    43981 aattagattc caatcaagca ataatctctg aactgacagt aggcggtctt gtggacagtc
    44041 tggcgcttgc tgaaggtttt actggagaag atatttatga gatgttttca gaagtccgtc
    44101 agttaaaaaa aggagaaaaa tgcatagagg ttcatcatga gagcccgaat gtgaggttca
    44161 aacgtggtga taatgagatg atcttcaaga atcatgtgtc tgatcacact gaactatatg
    44221 aaatcgtgaa tcaaatactg tctgggattt ttgaattagg accttatgag ttcaaggaag
    44281 aaaacggtaa tccaaaatta aatgaagaat ggcaagaaac gattgaaaca caaagaaaca
    44341 atccacactt atttcaatag tcttccacag tatttcgtaa aaatgttgat gaaaaagaaa
    44401 caccgtgaat cagaggtcgt gaaaaacctg ccctgcatca agtaataaga acagccaaac
    44461 aaaaatagct cctcctattt ttcgtttggt tgggcttatt accgcagatg caagtttttg
    44521 aacaccgttt aaggaagaga ctgtgaagtc aacgttctga cctgagtatt agagagacaa
    44581 atggcaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    44641 tgaacaccgt tttatcgaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
    44701 ttttgtgaaa atggctttcc acatttttgc ataaatttga cttaataccg agaaaatggc
    44761 ttttgaacac cgtttattca aaaaaaggga ctttgacact acttttgtca aagtcccatc
    44821 ggttttttat ctatttttat tacaatcatt tcttccagag tagtgacatc tggaatggct
    44881 gaaatgtaaa cagattattt gcctgtatca atgtcgaaga gtaatataaa aagtgtaaac
    44941 gctatcatat tgttcatcat tcaaatttta tagagagaag gcattgagat ggaaaaaaca
    45001 tatcaagcag gtacaaatga aggaatagta gattttatca atatggaaga tttagaaatc
    45061 gcggcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg tgctggcgac
    45121 ttatttactt atcaagaaaa tgaacgggca ttcaatcatc gtttgattat tcctcatgta
    45181 ttaagagatg tagaattacc cgatacaact acacattttg acgaagaaat gctgactgca
    45241 ccaatcatca tggcaccagt tgctgctcat ggattagctc atgtgaaggc ggagaaagct
    45301 tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta cgcttcttgt
    45361 actttagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt tcaattttat
    45421 atgagcaaag ataatggaat caatttggat attcttgaag tggccaagcg taatggagca
    45481 aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac agaccgcaga
    45541 aacgggttta ccttcccgct gccaatgccg atcgttcagg cttatcaatc aggagtaggc
    45601 caaacgatgg atgctgtata taaatcttcc aaacaaaaac ttagtccaaa agatgtagaa
    45661 tttatcgctg ctcattctga tttgccagtc tacgtgaaag gtgttcaatc agaagaagat
    45721 gtctatcgat cattagaatc tggtgctggt ggaatttggg tatctaacca tggtggccgt
    45781 caattagatg gggggcctgc tgcgtttgat tcgttgcaat atgtggcaga agcagtagat
    45841 aaacgtgtac caatcgtgtt tgacagcggt gtacgacgtg gacaacatgt tttcaaagca
    45901 atcgcatcag gtgccgatct tgttgctatt ggcagacccg ttatttatgg attgtcttta
    45961 ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt ggaaatggtc
    46021 atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt gcgtgagaat
    46081 cgttttattt aagaaataaa tgacgaaaaa aataggagtg cggcagatga tgccgtactc
    46141 ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa aataaaattg
    46201 aaatattttt tcatacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt
    46261 ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt
    46321 ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa
    46381 aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc
    46441 agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa
    46501 taccgttgag cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga
    46561 aaatccagca actggccaga caggttcttt aacattggat gtagttcctg aattgccatt
    46621 tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga
    46681 tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg
    46741 gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc
    46801 tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc
    46861 aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc
    46921 agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact
    46981 gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc
    47041 tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt
    47101 gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa
    47161 cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc
    47221 caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata
    47281 gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa
    47341 gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg
    47401 gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca
    47461 ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca
    47521 catgcagaaa caactatacc tatagaagtg aaaatcccag caaaaagctt taagggacgt
    47581 gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa
    47641 ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa
    47701 gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag
    47761 ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt
    47821 acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa tgaattttta
    47881 gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc
    47941 ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca
    48001 caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca
    48061 gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta
    48121 tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga
    48181 tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt
    48241 cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta
    48301 tgatctaagt ccagttccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac
    48361 acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaga
    48421 aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt
    48481 gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt
    48541 ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg
    48601 cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata
    48661 tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg
    48721 gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt
    48781 ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg
    48841 atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac
    48901 atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg
    48961 ccggtgcccc catcaaggtt actagcggta gtttattatc agcaggaagc cataccttgc
    49021 gttttgggat ggggtcgtgg gatacaaata ctctattggc agatattatt gctattatta
    49081 cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg
    49141 cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg
    49201 gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga
    49261 ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt
    49321 atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag
    49381 agataaatac gtggaatagt tacatctcaa caagtgattc aacaggaaaa tataatacat
    49441 ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata
    49501 gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct
    49561 ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc
    49621 atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag
    49681 atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag
    49741 acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt
    49801 ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc
    49861 attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt
    49921 tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat
    49981 tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt
    50041 atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa
    50101 taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac
    50161 aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga
    50221 cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc
    50281 ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata
    50341 ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca
    50401 ttcatcttac ttacaactat caattaaata ctgtagatac ttctgtacaa tccgtcagca
    50461 tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc
    50521 ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat
    50581 tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa
    50641 gttattatga agcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca
    50701 cgtctccttg gcaactgaca gggacattga cttctttatt taagaatgat caaggtcaag
    50761 aattaagcgg aacaaaatta tacttcaatc attctggatc aaaacaattg attcagcaag
    50821 gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact
    50881 ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg
    50941 caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc taagtgatta
    51001 gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa
    51061 cttctgctgt taaaatcaga aatgcttgga ggagtggact tggaactttt cagtttgctt
    51121 gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag
    51181 atttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat
    51241 ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa
    51301 gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgc
    51361 tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt
    51421 gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg
    51481 aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc
    51541 ggtccagaaa gtcaaatttg ttatttttat ttccaattat tttggtttgg acgtccttat
    51601 cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa
    51661 aaagagttta gttttactat tacagaagaa ggacgattga aatttgaaat atggctttcc
    51721 attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct
    51781 gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac
    51841 ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg
    51901 atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa
    51961 aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa
    52021 tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg
    52081 caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga
    52141 ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg
    52201 gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt
    52261 gaattatttg ctcgctatgc atctattttg cggacgctga tcaagcagtt agaattacgt
    52321 ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc
    52381 atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag
    52441 cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa
    52501 gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta
    52561 aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca
    52621 ttggtgcttc ccatgcaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag
    52681 cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa
    52741 tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga
    52801 ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag
    52861 acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata
    52921 aattaatcgt agcaacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa
    52981 atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag
    53041 caaaagaagt agtgatcttc ggtgctggtt atattggatt ggaattagca gatgctttga
    53101 gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt
    53161 tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa atcaattttt
    53221 atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac
    53281 ttctatctca agcaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata
    53341 cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat
    53401 atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg
    53461 acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag
    53521 cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag
    53581 cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat
    53641 tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgatg
    53701 aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg
    53761 gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg
    53821 tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaac
    53881 acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa
    53941 atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat
    54001 ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt
    54061 tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta
    54121 tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg
    54181 ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg
    54241 gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc
    54301 gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat
    54361 agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc cagacacctg gagtcgtgac
    54421 tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa
    54481 tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt
    54541 aggcgatatt agtttgaaag aagaatatgg tctagccgta ggagatacag ttaagttatc
    54601 tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc
    54661 accagttctc tatacaacca tcaatgctta ccaagagatc cgcttcgaaa aagaagatac
    54721 aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga
    54781 agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca
    54841 agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg
    54901 gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca
    54961 agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt
    55021 tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc
    55081 ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgctggtagt
    55141 agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc
    55201 aattggataa gaaggaggaa gagcatgaaa gcaatagaat ttaaagaagt agaaaaaagt
    55261 ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa
    55321 ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg
    55381 gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt
    55441 aatcaaaaag aaagtgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa
    55501 gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt agataagatc
    55561 aaacatgaac atcgtcaaaa agaagcaaaa gaattatttg agcagcttgg cgtggataag
    55621 ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct
    55681 cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat
    55741 tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa
    55801 gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg
    55861 atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca
    55921 tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc
    55981 caagggatat ctcggcactt caacaagaga gatcgcacaa aaagttggga tcacccaacc
    56041 caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca
    56101 cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca
    56161 agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct
    56221 gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct
    56281 atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct
    56341 aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta
    56401 catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt
    56461 atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa aaatagttag
    56521 gtgaagaagc aagatatgac gaagatcgtt atatcttgtt ttttttatat aggtagtttt
    56581 tcataaatac aaatttgtta ttctctcatg attatcaggg tatattttta tttattatgt
    56641 cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg tagaatgaag
    56701 gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt tcaggatatg
    56761 ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat atcttttaca
    56821 gccccttttt tgcgtactta agctgatttt aagttttgac ccttagtctg gatctacgag
    56881 gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca ttatcattat
    56941 tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa cggtcagcag
    57001 tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac aagagacatt
    57061 aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa ctaccccact
    57121 tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa atgcttatca
    57181 gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa ccaaagtcac
    57241 aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat tagatcctgg
    57301 gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag aaaaagatat
    57361 aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag caacggttta
    57421 tttgacccat actacagatg aattagtaca gcttggagat atatgcgact acagtgaaga
    57481 aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg cgaatgaagc
    57541 aactggaatc acgacatatt attactatgg acaagaggaa acattagcac agacaattgc
    57601 tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga actatcaagt
    57661 tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga ataatgatag
    57721 tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa gtttgacaca
    57781 agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat agctcctcat
    57841 atttttcgtt tggccgggct tattatcgca gatgcaagct tttgaacacc gtttaaggaa
    57901 gaggttgtga gcctgccgtt taactccgcg tcacaaggag caatttcaaa aaacagcttc
    57961 tcatgtttga tgactattgt gacttgtgac cgaggagttg gcatgagaac accgtttatt
    58021 cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt agcagcttca
    58081 aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc
    58141 ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct gatggaaggg
    58201 tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca cgactgatga
    58261 tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt
    58321 cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact
    58381 gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg
    58441 cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt
    58501 atttttctgg agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa
    58561 gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt
    58621 ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggacca aaatagctgc
    58681 catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag
    58741 aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta
    58801 ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc
    58861 cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat
    58921 agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa
    58981 aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag
    59041 agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa
    59101 tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa
    59161 ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcag tgattgccaa
    59221 ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct
    59281 gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc
    59341 agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatctt
    59401 ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga
    59461 aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa
    59521 tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa
    59581 acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa
    59641 aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt
    59701 attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc
    59761 gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca
    59821 tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag cattttttga
    59881 tcaggtattt gcttttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat
    59941 tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta
    60001 tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc
    60061 ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag tgtggtccaa
    60121 aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc atttcttacc
    60181 aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata gtgctggaaa
    60241 atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt atgtccggca
    60301 cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat ccttgaaaca
    60361 ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc acaaggaaaa
    60421 gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa ctggcttcaa
    60481 gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat acctaaactt
    60541 gaaaatatcc gtccccatat gaaacggatc gagatcggag cagacttgaa tggagtggag
    60601 cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt cattgatgat
    60661 ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca gttagtcacg
    60721 atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg cgtgactgat
    60781 gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga acaagctgtt
    60841 cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc
    60901 atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg cggtgtattt
    60961 ggcggtgtag tccacgatca aagtgcttca ggacagactt tatttattga accaaaacaa
    61021 gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa
    61081 cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat catccacaat
    61141 gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg taaagaagta
    61201 aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca agcaagacat
    61261 ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa agactatcaa
    61321 gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa gactttaggt
    61381 ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga aagtcaaatc
    61441 ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga gcaaagtcta
    61501 tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt caatgaaaaa
    61561 agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg tgctgctcta
    61621 gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc aacaacgcat
    61681 tatcctgaat tgaaagttta tggatacaat cgggcaaata cgattaatgc cagcatggag
    61741 ttcgatgtgg atacactaag tccaacgtat cgtttgctga ttggcgttcc tggacgaagc
    61801 aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga tgaagcgaag
    61861 cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt agagaatcgc
    61921 cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga ggcacaagaa
    61981 cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga aaaagaaatg
    62041 gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg cggaagaaaa agcagaaaaa
    62101 atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt gaaagagcat
    62161 cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac tttgaagaaa
    62221 aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg
    62281 gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca gtggcaagta
    62341 gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa
    62401 aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag tcacgtgccg
    62461 aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt agatcaatat
    62521 ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg aaaaggaaca
    62581 ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt caaaagcttt
    62641 gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc
    62701 ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac taatcataga
    62761 ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca tttaatgaag
    62821 aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta
    62881 tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa ttcagaattg
    62941 ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc atgagcattc
    63001 caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt gttcattcaa
    63061 aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca aaaataagct
    63121 gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt aaggttattc
    63181 aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt tgacagacat
    63241 gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac
    63301 aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa aaatggaacg
    63361 atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata ttttactggc
    63421 agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga atacatcgtt
    63481 acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa aaatgatcct
    63541 aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat aacgaatgaa
    63601 aaatatcccc gtttggtgat tactcgtaaa gtactaaaag acaaagcact gtattttgga
    63661 ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg attatttcct
    63721 ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca tttgggacaa
    63781 tgtctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa
    63841 gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt cagagatcca tcagaaaatg
    63901 gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga ccagatccgt
    63961 gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact ggatcgagat
    64021 gtatttggtt atgcgattga caaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa
    64081 ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc agaagaagat
    64141 ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc taaagaagtc
    64201 ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac gaaagtgctg
    64261 caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgaataagaa tgcctcagtt
    64321 gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat cggagcagtt
    64381 gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc ttttgataac
    64441 tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga tggaaaaccg
    64501 gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga tgattatgca
    64561 tcaatgagag aagtcatcta tcgcagatat tcacgagtga ttcgcgaagg gttgccttta
    64621 cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa agatgtctta
    64681 gaaaatcaat taggaatcga tattcctatt gctggcatgg caaaaaacga taaacataaa
    64741 acaaatgagt tgctattcgg tatagatttg gctgtagtac cgcttgagcg aaattcacca
    64801 gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat cactttccat
    64861 cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga aaggttagga
    64921 ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc
    64981 acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat
    65041 aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt cctatcattt
    65101 tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc ttacgtctgg
    65161 tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga agtcaaggat
    65221 cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca aatccccgat
    65281 ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac ttttcgtttg
    65341 atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca tcatcttggg
    65401 gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta tccgaaagta
    65461 acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag aaaagagatt
    65521 gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa
    65581 gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac gttaatccat
    65641 gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt aaatgctgaa
    65701 ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat tttttctagc
    65761 cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa
    65821 atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac gaagttattc
    65881 ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc
    65941 cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg gaaagaaatc
    66001 tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc gccaactttg
    66061 gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga ttattaccaa
    66121 agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat tagtcccttt
    66181 tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata gtgatgtcaa
    66241 taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc aagtcaaaac
    66301 agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag agctttcgga
    66361 agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa atgggatgct
    66421 ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac aactgactgg
    66481 cttgcaatcg atgcttcgag caagtcaatt aggcatttca ccagaagaag tagctagctt
    66541 gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac
    66601 agttggagaa gataactcga cagtccaata tgtcgtaagt tatgtagcaa cgattgtctt
    66661 gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg
    66721 cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt tttacggaaa
    66781 actaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta ttatttttgg
    66841 tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag acggtatctc
    66901 attggataac ttgattggac cattcttgtg gtattctctt ctattcatga tctttggcat
    66961 tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag cagaagatac
    67021 agcaaaagca attttgccag tgacttatct atctctaggc ggatatatgc ttggattgat
    67081 actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata ttccattctt
    67141 atcttcatat attatgccgg ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt
    67201 gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt ctgcaaacat
    67261 gtacaaatcg aatgtactcg tgtatagcga aggcggatta tggagttcat tgaaacaatc
    67321 gatttcaatt atgagaaatg aacgaaaaaa agcataaaat atagaaaaat atagaaggaa
    67381 gctggacatt tattgttccc agcttctttt ttgtatagac ctatttctat ttttttatta
    67441 tattggatat atatattcat tgaaaataga gcataacaac tttataatgt tgagtatacc
    67501 aatttttgga ggcgttttaa tggaccgcta tttagattta ctttcagaga agttccctac
    67561 aacggaagat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg
    67621 aacagagtta tttttgagcg atatacatgg cgaatttcct gcttttgatc atattctgcg
    67681 tatcggttca ggaaatctta aagaaaagat caaagacctt ttctctgaac aaatgacgga
    67741 aacggagatc agccaattca ctatttttac ggcttatcca gaatatgtgt tgacgactga
    67801 ttggtacaaa aaacaagata aatcccagct gatccatcgt ttgatcgatc ttttacggtt
    67861 tacgacagtc aaatacactc gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta
    67921 tatcattgag gaactcttgt atatcgatga tcgtatcaac gggaaaaaag actatgtaaa
    67981 aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc
    68041 gcagacgatc caaaaatctg tcatcgatca tttgcatatc gtgggtgata tttttgatcg
    68101 aggaactcaa gcagccaaag taatggatcg aatcatggat ttttcctctg tagatatcca
    68161 gtgggggaat catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttatt
    68221 gacgctttta cggattgccg cacgctacaa ctatttattt gaattagaga aagaatatgg
    68281 attaaattta cgtccattat tttcttttgc ccatcaaacc tatcagaaga atccagtatt
    68341 tacccctaaa aacaggaaag atctatctga aagagagcaa gaagaattag aaaagatcca
    68401 tcaagcactt tctatcctcc aattcaaatc agaacatcag ttgttgaatc gacgacctga
    68461 atttaaaatg gacgatcgta aattgttgga aaaaatcaac tatgaggaat caacgatcgt
    68521 tttagaaggt cagctctatg agttgaaagg gacttgtttt caaacgatcc agccagatga
    68581 tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat agtttgatgt attcattcca
    68641 gcactcgtta agaatgcaaa aacacatgac gtttttgatc gaaaaagggg gaatgtatct
    68701 cacgaacaat cagcatgtac tgttccatgg ctgtatacct gtagatgaaa aggggcagct
    68761 cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa
    68821 acagataact gaaagcgcaa aagatttgac tgcaaatgaa gatttggcga ccgatttgat
    68881 ttggtatgcg tggagcgggc cattttcgcc tttattcggc aagagtaaaa tggcaacttt
    68941 tgagcgctat tttctcacag agcctcatac gcacgtcgaa aaaagcaatc cctattatca
    69001 cttgcgagat gaagagtggt tcagtgaaaa aattttggca gaattcgatg caaaagaaga
    69061 aggttcagca atcatcaatg gacacacgcc agtaaaagta aaaaaaggag agtcaccgat
    69121 caaagcaaac ggaaaagcat ttgttattga cggaggtcta tccaaagctt atcaaaaaac
    69181 aaccgggatt gctggctact cattactatg caattcttac ggtttccaaa tcgtgacaca
    69241 ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa
    69301 aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg aatacgacaa aaggaatgga
    69361 attacagcaa cagattactc aactccaaag gctgttggat tatcgaaagg acgattgaga
    69421 aaaatgctag ccaacattgc tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc
    69481 agtctccttc ggaattagct caaaaaacgg taaaatatta gaagctattt ttctgtttat
    69541 ttctctaata ctcaggtcag ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc
    69601 tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa
    69661 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctaatcagaa aaacgattaa
    69721 aattcattca caaagaaatc gaataaacgc tgttcagatg aatctacacg atgagaaagt
    69781 tccggatgcc attggacacc aaggattttg tgcatattat ctttactttg gactgcttca
    69841 atgagtccat cgttggaaaa ggcgatggct tttaacaaag gagaaagatc tttgactgcc
    69901 tgatgatgat aggaattcac aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc
    69961 gaaacgattt tgacttcgtg agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt
    70021 acggaccatt ttggatataa tgaaagatct tgataaagtg ttccctccaa cacgacattc
    70081 aatagttgaa gtcctcggca gactgcaaaa atcggttttc tttgtttcaa tgcttccaag
    70141 atcaatgcct gttcgaattg gtctcgttga atatttgttt ccgtcagttt tggatgaggg
    70201 tcttccatgt aaagttgcgg actaacatcc tgaccgcctg caagaatgag tttgtcgatt
    70261 ttctcaatta gttgaggcgc tgaatctgga gacatgactg gcagaataag aggtaatcct
    70321 tgagcatcta aaactgcatc cacaaatccc tgcggtgtat agcttacttg atttccttgg
    70381 aaagtatctg tcgctcgaat cagttgattc ccggcaatgc cgatgattgg tttcattttt
    70441 attcccccta aagtagacta attgtaacaa ggatttaaaa aaatttaaac tatgtaagac
    70501 ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaat tttcgtgtga
    70561 aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt aagcagaaat
    70621 aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa ggacatttta
    70681 ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa tgaaaaaaga
    70741 aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa gctttggaac
    70801 aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg aaccctacgg cagcagttga
    70861 agacctatgc agaagaaaaa gagcaagaat tggagataat ccaaacgaac agtcggcaac
    70921 agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa
    70981 aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagac caatatcaaa
    71041 agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc
    71101 actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa
    71161 gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt atcaaaatag aacaaacaac
    71221 cagtgggtag aaaaatattt tccagatacc acgattattg ataaagaagc aaaattaagc
    71281 gctttaggca atcggctgaa agacatccaa gaagctatcc aattcataca acaccacggc
    71341 gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agacttggaa
    71401 gcaaattatt ccgaagaaaa tttcatgaat aaattagaag aagtccttga tttaaaagaa
    71461 aagtgggaac agctaactga aaaacgtgat cgtttagaag aagaggaaga atggcttctt
    71521 cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac catttttgag
    71581 atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactaag taagcttgat
    71641 gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc gtatgttgct
    71701 ttaaaaaaat cagttgctgc tatagaagaa attgctggga aatacgggtt caataaagaa
    71761 acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc
    71821 attgttcacg agcagcaaaa aaagataacg acagcgttag gtcagtgcag cggctatatc
    71881 caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacggga aaaagtaaaa
    71941 gaacggttta tccatgccac ttatctgatc gttttgcaag gttgggtaga tgcagaagaa
    72001 aaacagaatt tgatgcatgt gttaaacgaa actgtatcag aagaagatat atacgttagt
    72061 ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt gaaaaaccat
    72121 ccgcttatcg aaccttttga attgctgact gaaatgtaca gtctgccaaa atatgaagaa
    72181 gtagatccaa cgccttggat gacaccattt tatcttgtct tcttcgggat gatggtcgca
    72241 gatgttggtt acggattgct gatgctcatt gggacaatac tagctcaaaa actatttgtt
    72301 ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcggt accatcgatc
    72361 atctggggat tgatctacag ttcatttttc ggacaggcat taccaaaaga actatttggt
    72421 atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat tttgatttta
    72481 tccgtgattt tcggtctgat acagatcttg gttggattat tcgtggcagc aaaggaaaat
    72541 atcaggagga aagattactt gagtgctgtc agtgatggtt ttgcctggca agggatcctg
    72601 attgggatcg ttattgcatt gattggtgtc atgttgataa aaaatacagc atttgtttat
    72661 cttggcgcgg ggctatcgat cattgctgct ttatgtatct taatcgtgcc gattatccaa
    72721 tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata atctttacgg attgactggt
    72781 tatatcggtg atttagtcag ttatacacgt ttgatggcac taggtatttc aggcggaagt
    72841 attggcgcgg cattcaatat gctagttgct ttcatgccgc cagccgctcg attcagtgta
    72901 gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact gttaagtgcc
    72961 tatgtccatg gtgcacgatt gcagtatgtc gaattttttg gaaaatttta tacaggaggc
    73021 ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa
    73081 aaaaacaata aataattgga ggatttttat acaatgatgg attacttaat tagtcaaaat
    73141 ggtggaatgg tatttgcagt attagcgatg gcaacagcga cgattttttc agggattggt
    73201 tcagctaaag gtgttggaat gacaggtgag gcagcagcag cgttaacaac aagtcaaccc
    73261 gaaaaattcg gacaggcttt gattctgcaa ttgcttccag gtacgcaagg gttgtatgga
    73321 ttcgttatcg cctttttgat ttttatcaac ttaggaaatg atatgtcagt tgtacaagga
    73381 ttgaactttc taggcgcttc tctgccgatt gcttttacag gtttgttttc aggtatcgcc
    73441 caagggaaag ttgcagcagc aggtatccaa attttagcga aaaaacctga acatgctaca
    73501 aaaggaatca tttttgctgc gatggttgaa acatatgcga ttttaggttt cgttatttcc
    73561 ttcctattag tattgaatgc ctaagcataa aggaggaaaa gcagatgaac gccattgaaa
    73621 aaatcatctc tcagatgaat gaagcagcag agcaagaaag agcggcgtta gagcaagaag
    73681 agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaaatcgaa acagaacatc
    73741 aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc
    73801 gccaacaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat
    73861 tgtttgcaga tgcaatagct gaaatggaga attgggatga atctgaacaa atccagttta
    73921 tcaaaaattc attgtattca ttacctttga caggcaaagt aacattcatt gctggagaaa
    73981 aatcagcagc ttatctttct cagacattgc tggatgaatg gaacaatgaa cttcccttta
    74041 tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata aacgatcaag
    74101 gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga acgatgagtt
    74161 ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat accacgaact
    74221 aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat ttgaacggat
    74281 gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga tttacagtcc
    74341 atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg agcagagccg
    74401 tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga tctatacgat
    74461 gcgctatact ttccataatc tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct
    74521 ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg atgccattca
    74581 tacgcaagtt tcaaatgaac tccctgaaag cattatggac agtatacgag aagtacgtga
    74641 ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc gacagttttt
    74701 gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga cagaagtcat
    74761 tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc agaaaagaac
    74821 atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag aagtattttt
    74881 gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga cagattatgc
    74941 tgattttctg cgccctgtca tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt
    75001 gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt tcggaccact
    75061 gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt
    75121 tatcggcaaa agaagccatt tcgaaccaga aacaatcaga aagcgggtga gacaagtcta
    75181 tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc aaattgtttg
    75241 gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg
    75301 caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg agcccagaaa
    75361 cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc aatcatcaag
    75421 gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa
    75481 atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct
    75541 ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac atgtgtctag
    75601 tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc
    75661 aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct actggtgaag
    75721 ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga atccaacggc
    75781 cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga gttcaattgc
    75841 ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg
    75901 tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa cataaaatca
    75961 tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca ttcactattg
    76021 atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa
    76081 aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat gcacccatga
    76141 tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga gcagcagctg
    76201 tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca aaatgggccg
    76261 atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt
    76321 taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg gaacgtacgg
    76381 tattgattgc taatacatca aacatgccag tagcagctcg agaagcttct atctatacgg
    76441 gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc atggcagact
    76501 ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg
    76561 gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat gaacgatctg
    76621 gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca atcagtgctg
    76681 tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg cgggtcgtaa
    76741 aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct tcgatcgatt
    76801 ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat catatccttc
    76861 aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc
    76921 tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac cgtctgacat
    76981 tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt gatgatgtcg
    77041 atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg acctttggtg
    77101 aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg gacggtaccg
    77161 tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa ttagaaaaac
    77221 tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca
    77281 tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt
    77341 gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca aaatggcgag
    77401 attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca aatattcgaa
    77461 ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca tcctttagaa
    77521 ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg accgaaagat
    77581 aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt aatcaatcca
    77641 gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat cgatcatttg
    77701 aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct tcctcataaa
    77761 gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga tgatttcgcc
    77821 gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat ggaagatttt
    77881 cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa cgaccctgca
    77941 atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct tgcttatgaa
    78001 aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga ggcattgcga
    78061 gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg gtatctttac
    78121 acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa aggatcggtt
    78181 actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat ccctgacttg
    78241 actggttata ttacagaagg acagatcatc ttgactagag agctttacaa gagtgggatt
    78301 cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg aactggaccc
    78361 ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc ctatgcacaa
    78421 ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc tgatgtagac
    78481 aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca aggattctac
    78541 acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc gatgttgcca
    78601 agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc agaagggaag
    78661 tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac tcgattgaaa
    78721 aaacagttga caactgccac aagagggcat aaattattaa aagataagca agatgaactg
    78781 atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga aatggaagag
    78841 tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac ggaacctttc
    78901 atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt agacaaaaat
    78961 atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct gacagacaca
    79021 cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat cgatcggttt
    79081 actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg tcaactgatg
    79141 gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat gacgatccct
    79201 caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa
    79261 gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt atcttttttt
    79321 aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg ccggctgttc
    79381 agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta cttaccctac
    79441 cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc actgcaactt
    79501 ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg aacagtgctg
    79561 gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg atggttccga
    79621 ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt atattaaaag
    79681 aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac atcctaaaat
    79741 tggcctttgt tatccaattg attggtacgg ttgctttatc ttttgtattt gtgcctgaat
    79801 ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt ttttgtaatg
    79861 cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt tatttgatca
    79921 tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg cgtgatttac
    79981 tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta accgttacag
    80041 gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc
    80101 tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt gtgacaccta
    80161 gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt ctgattttaa
    80221 cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa
    80281 ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt acgagagcgg
    80341 aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact ttattttttg
    80401 ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa acgataccta
    80461 aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt acagtaggtc
    80521 ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt atagcgctta
    80581 tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca aaagcacaaa
    80641 gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa tttcatcttc
    80701 tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct tgcttcttgg
    80761 atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt tgaacctcgg
    80821 gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat ttatcaatag
    80881 gttataattc atattttttt attttttctt ttacgatact tagtccagaa gctaaaattt
    80941 caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa
    81001 agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca attatcttag
    81061 ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc gttggttttt
    81121 ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt aatttagttt
    81181 ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc acatctaatc
    81241 cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg acatgtactg
    81301 gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt ccagctaagt
    81361 agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt tgatagtaag
    81421 ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt tcaatcactt
    81481 cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta ttacaagaag
    81541 gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat atatcaggtc
    81601 gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac tgagccaatt
    81661 caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga gagaaaagtg
    81721 cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga gattctaagg
    81781 acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga aaatttgtgc
    81841 ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta gttgattgaa
    81901 taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca aaatcaatga
    81961 tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg ggagaaataa
    82021 atgtgccact tccaatattc gtatacagca aacctttctc aattccatat ttataagcct
    82081 tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg gagggtaact
    82141 gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga atcaacgatc
    82201 gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg taattgtcat
    82261 aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat tttaccattg
    82321 attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg aaaattatta
    82381 attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta atgaaaaaat
    82441 gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg gaaattttac
    82501 ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg gagaacagat
    82561 attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat ttaccttctc
    82621 atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca ttgataaacg
    82681 caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca gattacttag
    82741 caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt
    82801 tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat attttagaag
    82861 aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata acgtttataa
    82921 atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg ttgacctcta
    82981 gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc aatgctgtgg
    83041 gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact tctcaaatct
    83101 atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata gccatggcag
    83161 aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt tctgagaaaa
    83221 aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt ctagctattg
    83281 aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct ttaaaatgaa
    83341 cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa atccagcagc
    83401 ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg ccattgaaaa
    83461 caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt
    83521 tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag catttgtttt
    83581 atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa gcggtaatct
    83641 atttgtgaca agaattaaag attattatta catggatttt cctagtatta tgccgaaaaa
    83701 agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag aagcctactt
    83761 agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat tgcagccaga
    83821 ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag cagaaagtgt
    83881 gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt
    83941 atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa
    84001 attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga
    84061 tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt
    84121 tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc tacaatggtt
    84181 gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca ttttaattat
    84241 tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat cctcggatga
    84301 cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca gtgccaggta
    84361 atccttttaa gtcagtaatc tcaagaccgg cagctttatg ttgctcgtgt aatttttcta
    84421 gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat
    84481 catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa taaacaagtg
    84541 taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat
    84601 aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc attttcatag
    84661 attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga aatagttgat
    84721 ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa ctgttaaggt
    84781 ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa
    84841 cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta atcgaggctc
    84901 caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg aaagaagaag
    84961 cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg
    85021 gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat
    85081 atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa
    85141 tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg
    85201 cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct
    85261 ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg
    85321 tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt
    85381 gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc
    85441 atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta
    85501 cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta
    85561 tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc
    85621 atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc
    85681 ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg
    85741 tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg
    85801 atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc tgatttcgtt
    85861 tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac
    85921 tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca
    85981 ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca
    86041 tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat
    86101 atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa
    86161 gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc ttttggaaag
    86221 aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt
    86281 gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct
    86341 tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc agtgtaataa
    86401 acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc
    86461 cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa
    86521 tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca
    86581 tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat
    86641 tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc
    86701 agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac
    86761 atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg
    86821 atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat
    86881 tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga
    86941 ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc
    87001 ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa
    87061 gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga
    87121 gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc
    87181 tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg
    87241 cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct atgttctgct
    87301 ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg ttggacttct
    87361 ttcgaattga tcaaatctaa gattacttga tgctggacat ggatatagtt atggactgga
    87421 tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag
    87481 ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg
    87541 gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc
    87601 aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc
    87661 ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc
    87721 ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt
    87781 gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc
    87841 aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc
    87901 gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta
    87961 agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag aaatattcaa
    88021 aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc
    88081 tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc
    88141 taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga
    88201 atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg
    88261 ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc
    88321 tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg
    88381 gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga
    88441 gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta
    88501 aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt
    88561 ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct
    88621 gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg
    88681 atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt
    88741 atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca
    88801 ggagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat
    88861 tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga
    88921 ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt
    88981 gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa
    89041 cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgggaaa
    89101 tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc
    89161 agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg
    89221 atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt
    89281 aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac
    89341 gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat
    89401 gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat
    89461 gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata
    89521 gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa
    89581 taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag
    89641 gtgtgacacg at
//
LOCUS       DANJJW010000002        82135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000002 DANJJW010000000
VERSION     DANJJW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            20..1390
                     /locus_tag="PWS82_000091"
     CDS             20..1390
                     /locus_tag="PWS82_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905253.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(1630..1713)
                     /locus_tag="PWS82_000092"
     tRNA            complement(1630..1713)
                     /locus_tag="PWS82_000092"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1677..1679),aa:Leu,seq:gag)
     gene            1768..1947
                     /locus_tag="PWS82_000093"
     CDS             1768..1947
                     /locus_tag="PWS82_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905254.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            2096..3313
                     /locus_tag="PWS82_000094"
     CDS             2096..3313
                     /locus_tag="PWS82_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0905255.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3497..4333
                     /locus_tag="PWS82_000095"
     CDS             3497..4333
                     /locus_tag="PWS82_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905256.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4409..5557
                     /gene="nagA"
                     /locus_tag="PWS82_000096"
     CDS             4409..5557
                     /gene="nagA"
                     /locus_tag="PWS82_000096"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL0905257.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5777..6835
                     /locus_tag="PWS82_000097"
     CDS             5777..6835
                     /locus_tag="PWS82_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL0905258.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6839..7873
                     /locus_tag="PWS82_000098"
     CDS             6839..7873
                     /locus_tag="PWS82_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL0905259.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7975..8691)
                     /locus_tag="PWS82_000099"
     CDS             complement(7975..8691)
                     /locus_tag="PWS82_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0905260.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8660..9445)
                     /locus_tag="PWS82_000100"
     CDS             complement(8660..9445)
                     /locus_tag="PWS82_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL0905261.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9436..10185)
                     /locus_tag="PWS82_000101"
     CDS             complement(9436..10185)
                     /locus_tag="PWS82_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0905262.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10483..11007
                     /locus_tag="PWS82_000102"
     CDS             10483..11007
                     /locus_tag="PWS82_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL0905263.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            11023..12522
                     /locus_tag="PWS82_000103"
     CDS             11023..12522
                     /locus_tag="PWS82_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL0905264.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12610..13212)
                     /locus_tag="PWS82_000104"
     CDS             complement(12610..13212)
                     /locus_tag="PWS82_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0905265.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13316..14272
                     /locus_tag="PWS82_000105"
     CDS             13316..14272
                     /locus_tag="PWS82_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0905266.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14577..15533)
                     /locus_tag="PWS82_000106"
     CDS             complement(14577..15533)
                     /locus_tag="PWS82_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0905267.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15707..16267
                     /locus_tag="PWS82_000107"
     CDS             15707..16267
                     /locus_tag="PWS82_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905268.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16260..17084
                     /locus_tag="PWS82_000108"
     CDS             16260..17084
                     /locus_tag="PWS82_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL0905269.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            17094..17711
                     /locus_tag="PWS82_000109"
     CDS             17094..17711
                     /locus_tag="PWS82_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0905270.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17729..18412
                     /locus_tag="PWS82_000110"
     CDS             17729..18412
                     /locus_tag="PWS82_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL0905271.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            19095..20330
                     /locus_tag="PWS82_000111"
                     /pseudo
     CDS             19095..20330
                     /locus_tag="PWS82_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            20338..21225
                     /locus_tag="PWS82_000112"
     CDS             20338..21225
                     /locus_tag="PWS82_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0905272.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            21242..22078
                     /locus_tag="PWS82_000113"
     CDS             21242..22078
                     /locus_tag="PWS82_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0905273.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            22081..22704
                     /locus_tag="PWS82_000114"
     CDS             22081..22704
                     /locus_tag="PWS82_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905274.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22701..23993
                     /locus_tag="PWS82_000115"
     CDS             22701..23993
                     /locus_tag="PWS82_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0905275.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24352..24449
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24565..25146
                     /locus_tag="PWS82_000116"
     CDS             24565..25146
                     /locus_tag="PWS82_000116"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL0905276.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            25149..26456
                     /locus_tag="PWS82_000117"
     CDS             25149..26456
                     /locus_tag="PWS82_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL0905277.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26749..27237
                     /gene="purE"
                     /locus_tag="PWS82_000118"
     CDS             26749..27237
                     /gene="purE"
                     /locus_tag="PWS82_000118"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL0905278.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27230..28354
                     /gene="purK"
                     /locus_tag="PWS82_000119"
     CDS             27230..28354
                     /gene="purK"
                     /locus_tag="PWS82_000119"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL0905279.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28367..29662
                     /gene="purB"
                     /locus_tag="PWS82_000120"
     CDS             28367..29662
                     /gene="purB"
                     /locus_tag="PWS82_000120"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL0905280.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29842..30219)
                     /locus_tag="PWS82_000121"
     CDS             complement(29842..30219)
                     /locus_tag="PWS82_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905281.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30475..31203
                     /locus_tag="PWS82_000122"
     CDS             30475..31203
                     /locus_tag="PWS82_000122"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL0905282.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31203..31457
                     /gene="purS"
                     /locus_tag="PWS82_000123"
     CDS             31203..31457
                     /gene="purS"
                     /locus_tag="PWS82_000123"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL0905283.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31459..32130
                     /gene="purQ"
                     /locus_tag="PWS82_000124"
     CDS             31459..32130
                     /gene="purQ"
                     /locus_tag="PWS82_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL0905284.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            32131..34353
                     /gene="purL"
                     /locus_tag="PWS82_000125"
     CDS             32131..34353
                     /gene="purL"
                     /locus_tag="PWS82_000125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL0905285.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34338..35777
                     /locus_tag="PWS82_000126"
     CDS             34338..35777
                     /locus_tag="PWS82_000126"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL0905286.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35800..36852
                     /locus_tag="PWS82_000127"
     CDS             35800..36852
                     /locus_tag="PWS82_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL0905287.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36849..37427
                     /gene="purN"
                     /locus_tag="PWS82_000128"
     CDS             36849..37427
                     /gene="purN"
                     /locus_tag="PWS82_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL0905288.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37424..38959
                     /gene="purH"
                     /locus_tag="PWS82_000129"
     CDS             37424..38959
                     /gene="purH"
                     /locus_tag="PWS82_000129"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL0905289.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38975..40249
                     /gene="purD"
                     /locus_tag="PWS82_000130"
     CDS             38975..40249
                     /gene="purD"
                     /locus_tag="PWS82_000130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL0905290.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40371..41285
                     /locus_tag="PWS82_000131"
     CDS             40371..41285
                     /locus_tag="PWS82_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0905291.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41418..42983)
                     /locus_tag="PWS82_000132"
     CDS             complement(41418..42983)
                     /locus_tag="PWS82_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0905292.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42976..44427)
                     /locus_tag="PWS82_000133"
     CDS             complement(42976..44427)
                     /locus_tag="PWS82_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0905293.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44441..45457)
                     /locus_tag="PWS82_000134"
     CDS             complement(44441..45457)
                     /locus_tag="PWS82_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0905294.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45677..46765
                     /locus_tag="PWS82_000135"
     CDS             45677..46765
                     /locus_tag="PWS82_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL0905295.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46819..48345
                     /locus_tag="PWS82_000136"
     CDS             46819..48345
                     /locus_tag="PWS82_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL0905296.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48347..49552
                     /locus_tag="PWS82_000137"
     CDS             48347..49552
                     /locus_tag="PWS82_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0905297.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49809..50786
                     /gene="guaC"
                     /locus_tag="PWS82_000138"
     CDS             49809..50786
                     /gene="guaC"
                     /locus_tag="PWS82_000138"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL0905298.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            51050..52474
                     /locus_tag="PWS82_000139"
     CDS             51050..52474
                     /locus_tag="PWS82_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0905299.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52935..54044
                     /gene="pdhA"
                     /locus_tag="PWS82_000140"
     CDS             52935..54044
                     /gene="pdhA"
                     /locus_tag="PWS82_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL0905300.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            54047..55024
                     /locus_tag="PWS82_000141"
     CDS             54047..55024
                     /locus_tag="PWS82_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL0905301.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            55049..56692
                     /locus_tag="PWS82_000142"
     CDS             55049..56692
                     /locus_tag="PWS82_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL0905302.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56700..58106
                     /gene="lpdA"
                     /locus_tag="PWS82_000143"
     CDS             56700..58106
                     /gene="lpdA"
                     /locus_tag="PWS82_000143"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL0905303.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            58268..58543
                     /locus_tag="PWS82_000144"
     CDS             58268..58543
                     /locus_tag="PWS82_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL0905304.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            58549..59316
                     /locus_tag="PWS82_000145"
     CDS             58549..59316
                     /locus_tag="PWS82_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL0905305.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            59484..61313
                     /gene="typA"
                     /locus_tag="PWS82_000146"
     CDS             59484..61313
                     /gene="typA"
                     /locus_tag="PWS82_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL0905306.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            61574..63346
                     /gene="recQ"
                     /locus_tag="PWS82_000147"
     CDS             61574..63346
                     /gene="recQ"
                     /locus_tag="PWS82_000147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL0905307.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            63460..63762
                     /locus_tag="PWS82_000148"
     CDS             63460..63762
                     /locus_tag="PWS82_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL0905308.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63765..64928
                     /locus_tag="PWS82_000149"
     CDS             63765..64928
                     /locus_tag="PWS82_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0905309.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64954..68382
                     /locus_tag="PWS82_000150"
     CDS             64954..68382
                     /locus_tag="PWS82_000150"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL0905310.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            68403..69527
                     /locus_tag="PWS82_000151"
     CDS             68403..69527
                     /locus_tag="PWS82_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905311.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            69517..69954
                     /locus_tag="PWS82_000152"
     CDS             69517..69954
                     /locus_tag="PWS82_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0905312.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69938..70276
                     /locus_tag="PWS82_000153"
     CDS             69938..70276
                     /locus_tag="PWS82_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL0905313.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            70395..70955
                     /gene="rsmD"
                     /locus_tag="PWS82_000154"
     CDS             70395..70955
                     /gene="rsmD"
                     /locus_tag="PWS82_000154"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL0905314.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70952..71443
                     /gene="coaD"
                     /locus_tag="PWS82_000155"
     CDS             70952..71443
                     /gene="coaD"
                     /locus_tag="PWS82_000155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL0905315.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            71433..72476
                     /locus_tag="PWS82_000156"
     CDS             71433..72476
                     /locus_tag="PWS82_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL0905316.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72635..73363
                     /locus_tag="PWS82_000157"
     CDS             72635..73363
                     /locus_tag="PWS82_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL0905317.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            73479..73724
                     /locus_tag="PWS82_000158"
     CDS             73479..73724
                     /locus_tag="PWS82_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0905318.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73721..73939
                     /locus_tag="PWS82_000159"
     CDS             73721..73939
                     /locus_tag="PWS82_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL0905319.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            74075..74614
                     /locus_tag="PWS82_000160"
     CDS             74075..74614
                     /locus_tag="PWS82_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL0905320.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            74607..76880
                     /locus_tag="PWS82_000161"
     CDS             74607..76880
                     /locus_tag="PWS82_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL0905321.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76918..77964
                     /gene="holA"
                     /locus_tag="PWS82_000162"
     CDS             76918..77964
                     /gene="holA"
                     /locus_tag="PWS82_000162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL0905322.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(78053..78307)
                     /locus_tag="PWS82_000163"
     CDS             complement(78053..78307)
                     /locus_tag="PWS82_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL0905323.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78707..78976)
                     /locus_tag="PWS82_000164"
     CDS             complement(78707..78976)
                     /locus_tag="PWS82_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905324.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(79446..79901)
                     /locus_tag="PWS82_000165"
     CDS             complement(79446..79901)
                     /locus_tag="PWS82_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL0905325.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(80039..81967)
                     /locus_tag="PWS82_000166"
     CDS             complement(80039..81967)
                     /locus_tag="PWS82_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0905326.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26650 a        14079 c        17741 g        23665 t
ORIGIN      
        1 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
       61 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
      121 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
      181 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
      241 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
      301 agtattactt tgcaagtatg tttttgagaa aataaatgtt aaaaatttat tattggtttg
      361 ctctatatac tgtttaatac cattgacagt aatgggggaa accggttgga gagcaaccac
      421 cttgaactat cagtggccag tagcatttag tttactaact ttttatccgt tttttcaact
      481 cttaagagga gaagaaataa atagaaaaat atattgggta agtattcctt tattgatatt
      541 tttaactaat caagaacaag tcaacgcctg tttttttgtt ctaacaagta tagttagttt
      601 atatttgatt gtcaatggac gatataatta taagttatca gtattttcaa taataagctt
      661 agcagaatta attttctctt tgacaactcc agggaatgca ttacgtgctg cacatgaaat
      721 taataaatgg tttcctgaat ataaaaattt caactttctt aataagttag atttaggtat
      781 ttcatcattt ggaaagccgt tttttttaga catgaatatc ttatttttat tgctattttt
      841 cttaattttc cttctaacat ataggaaatg tcaaaactat tatgtgcgta tattgactgc
      901 attaccgttt ttcttaaacc tgattattta ttttggtaat acaatgggtc aaagctttac
      961 ttacgtcaat ggaaataaaa gggcaatgat ttggagtagt agtaacctga ataatctctt
     1021 tacagagtta ggaacgaaac tgtcactttt ttatccaggt acttggatag ctactttagt
     1081 agtcttggca ttattactat gtttaattgt tggaatttat ttaagttttg acaataagaa
     1141 aacttctatt tttttagtta ttttgatgat aatgggattt tgttcaaggt taattatggg
     1201 attttctccc acagtatggg cttccggaat gcggacgtac tatatacttt atgttgttat
     1261 cgccatctta gtactaatgg cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa
     1321 tgaatttatg caatttggat taaccgtgct tggtatttgt acctttataa taacggtgat
     1381 aaacagataa tatacaaaaa atccctcgct ttagtattag gtgagggatt ttactcgttt
     1441 ttcgattaat tgttttaaca cttgcaagtt tctacgagta gcttgtttcc gaccttcata
     1501 tactcacctc aggctgttta catgattgta cgttcttttt gtaaaggata cagtgatatg
     1561 tatataatgg cataaagaaa cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac
     1621 gagttgtagt gcagatgatg ggactcgaac ccacacgagc taacgctcac atgaccctca
     1681 atcatgcttg tctgccaatt ccaacacatc tgcgtaataa taacacacta atgatagcga
     1741 ggttttacat agttgtcaag ctcaaaaatg ggaggactta ttaagatgga gaaatatgac
     1801 ggagaatttt caggactggg aatgatctta ggtgtattga ttggacttgc attcggacgt
     1861 tttttgcttg gttttatgct aggaatcatt tgtggaattg caatggattg ggcagcgaat
     1921 ctatggaatg actatcatga caattaagtt ctcaacgctt gtttgtgttt ttttaatact
     1981 tacgtttttc ctaaataagt ggcaaaattt tctaggatgg agtatgatat tgaaatagta
     2041 aataacaaaa aatttggaaa gttctaactt tttgaaatga gggattggct aagtgatgag
     2101 agaatttgaa aaatccaaca aattagaagg tgtcagttac gatgttcgcg gtcccgttct
     2161 agaagaagcg gagcggatgc aagaagaggg cattagtatt ttaaaattga acacgggaaa
     2221 tcccgcacct tttggatttg aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc
     2281 ccgtgactca gaaggctact cagattccaa ggggatcttt tctgctagaa aagcaattga
     2341 gcaatattgc caactgaaac attttccgaa tgtgacgatc aatgatatct acaccggcaa
     2401 cggagtgagc gaattgatca cgatgtgtat gcaggggttg ctggacaatg gagatgaagt
     2461 actggttcca atgccggact atccgttgtg gacggcttct attgcattag ctggaggcac
     2521 acctgttcat tatatttgcg atgaagaggc agaatggtat cctgatattg atgatatcaa
     2581 atcaaaaata acgagtcgga caaaagctat tgttatcatt aatcctaata atcctactgg
     2641 ggcactttat ccaaaagaat tattagaaga aatcgttgaa gtagctcgac aaaataacct
     2701 gattatttat tccgacgaaa tctatgatcg cttagtgatg gatggtctag aacatattcc
     2761 gattgctaca ttagcgcctg atttgttcgt tgttacgctg aacggtctat ccaaatctca
     2821 tcgtgtagca ggatttcgag taggctggat ggtcctaagt ggagataagt cacacgtcaa
     2881 aggctatata gaagggctga atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc
     2941 tcagcaaatt atccaaaccg cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc
     3001 aggcggaagg atctatgaac aaagagaata tatctataat gcaatcaatg atattcctgg
     3061 tctttcagcc gtaaaaccaa aagctgcttt ttatatattc ccaaaaattg atacaaagcg
     3121 attcaatatt ttggatgatg aaaaattcgt tttggacttt ttacatgaac atcatattct
     3181 acttgtacat ggtggtggat ttaattggaa ccagccagat cattttcgaa tcgtttatct
     3241 tcctaaaatg gatgatttga aacaaacagc taagaaaatg agagaattcc tagcaaccta
     3301 tcgccaaaaa tgattttaaa aaccaaattt ttcagtagga gggccacaga caaacacact
     3361 gaggctctct ttctagtgga gttttcttaa ataactaaag tatattattt agttgcaagt
     3421 cttttcttga agtttgcgac ttcctaaagt aaaatacgaa tagaatgcga atacgcatat
     3481 tttggggagg atacttatgg aaacgttaat cgagtccttt ggaacgatca ttaaagagat
     3541 tcgaaaagaa caaaaaatga cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt
     3601 attaagtcgg atcgaaaata atgaggaact gccgaatgta ttggtgatga tgcagatttg
     3661 tcaacggcta ggggtaacga ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac
     3721 aaacgataaa ttatttgaac tgttggatag ttgtttccaa aaacgtgatt atcggaagtt
     3781 agaacaagta ctaaaatctt ctgatatttc tgaaaatctg tatcaagcaa ctgactttca
     3841 acgctattat tattatctcg gtgtttgcga attcacgttg cgtcaaaata tctctcagtc
     3901 cctttattat ttgaaagaag cattaagcta ctctagccaa aaagaaagaa tccatgtttc
     3961 tgacacagaa atacaactga ttagttgtat cggaaaaata tatggtgtgg caggaaaaac
     4021 ggctgaagca cgctactatt tgagtcgaag catccaactg ctgcatcaat cagctgatga
     4081 acgatcaaaa agtgaatttt cgcaaatttt ttataattat ggaaatttct tattccatca
     4141 aaatgaaatc gacgaagcgt tagaacaggt gaatcaagga atctattggg ctcaagagaa
     4201 gaatagctac tattacctca atgatttatt tgtgcttaaa agcttaatat acaagcgtaa
     4261 agaagaacca gaaagagcag cattctatga aggcttagca caagctgtga aacaaatttc
     4321 aaaaaatttg taaatggtgt agaccattct tgacttcttc tctaaataag agtactttag
     4381 aggtagaaaa acaaagggag agaatagaat gagaactttt atctatgctg acaaattctt
     4441 tttagcgtca ggtgtcaaac atgcaggcta tcttgaaatt accgatggac tttttggtga
     4501 atacaccaaa gaactacctg agggagaagt caacatcatt gaccaaagcg gcaaatggat
     4561 tgctcctggt ttggtagata cacatattca cggctatatg aatcatgatg taatggataa
     4621 tgatgcagaa ggcatcaaag tgatgtctga aggtctgtta tcttgtggag tgacttcatt
     4681 tttaccaaca acattgacgt caagcaaaga acgtttaaaa gatgtagctg agacaatcgg
     4741 caaaatgtat caggaagcac caggagctaa aatcaaagga atctattttg aaggtccttt
     4801 cttcacagaa gaacacaaag gggcacaaaa cccaagttat ttcggggatc ctgatttgga
     4861 tactttccat gaatggcaag aagcttcagg tggtttaatc aaaaaaattg ctttagcacc
     4921 ggaacgtcaa ggtgtagaag agtttgtcaa acaagtgact gaagaaggag tagttgtttc
     4981 tctaggacac agtaatgcta cactagaaga agcgcaaaaa gctgttgaag caggcgcaag
     5041 tgtatttgtc catgctttca atggtatgcg tggattgaat catcgcgaac caggtatggt
     5101 gggtgcgtta ttatctttag aacatgtctt ttctgaattg atttgtgatg gccatcatgt
     5161 acatccaaat gctgcgggaa tcctaatgga aaaagcagga catgatcatg tcgcattaat
     5221 tacagactgt atgatggccg gtgggatgcc tgatggaaat tacaatttag gcgaatttcc
     5281 agtagtagta aaagaaggaa cagcacgtct agaatctggc aacttagctg gaagtatatt
     5341 gaaattgaaa gaagcaatta aaaatgttgt agactgggga ttagcaacac cagaacaagc
     5401 agtcatgatg gctagtctga ttccagcaat cagctgtaaa atcgatgata catgtggaaa
     5461 aatcgctcaa ggacgcgatg cagactttat cgtattgaac cctgatatga cattggaagc
     5521 aacttatcta gatggcgtgg aacgtttcca cgcataatat aatttaagtt aaccttaaaa
     5581 ctaaacttct taagtttggt tttaaggttt ttttgtgttc tagtaaaaat cactttacat
     5641 aagtttcaaa agtttcttta atttcctatt gttcaataag taacaatgta aagataaaat
     5701 taaaaatgtt aatacgtatt agcaaaataa aagctttttt tattgtgaat aaatgaacat
     5761 aattaggagg agaaagatga acttatttga catttttcga tcgactttga cagatatgtc
     5821 gattgtcagt gcaatcactt caacagtttt tattatatta ttagggtttg tttgtagaaa
     5881 aaaggggatt ttttctgaat ctgttggaaa aattctatcg aaagtcgtct taacagtggc
     5941 tttgccagca ctggcatttc gagctttcat gcaagatatc aatacagaat tactgatgca
     6001 aggaatcaat gtcttgattt ggggattagt tatttatatt attcttattc tagtttcaaa
     6061 accttttttc ttaagctata aaggggataa gcaaacaacg ttgcgtgtcc tgacgatctt
     6121 tggttctacg acctttttcg gaacgcctat cgtcagtgcg atttatggtc ctgtcggcgt
     6181 gatgtattcc tcaattttca atattggcta ccgaatcttt ttgtattctt atggatacat
     6241 aaaaatgagt ggcttgaaga tggaagctaa aaatatcaaa gcgatgttcg tcaatccaat
     6301 cattatcgct acttttgccg gccttttcat ttggctgttc caagggtatc tgccacaagt
     6361 gtcagttaca ggtgcagaag gagagataag ccaagtagcg tttttgcgaa ttgaccagac
     6421 tgcaccgtgg ctatttcagc cgatggatta tttagctggt ttaagttcac cattggcgtg
     6481 gctagcaatc ggcgcaacat taggtgaagt aagcttcaaa aaagctgcat ctgaaaaaac
     6541 ttcatggtat tacagtctag tcaaagtggt atttgtacct gcattgaata tcgtgattct
     6601 agcagttttg acggcaacga atattctacc agtcaatttt gaagcactgg caacaatcgt
     6661 tatcatgatg gctacaccag ctgcaacagt ggcagcagcc tatgctatca gttttgatag
     6721 ggaggcactt ttagcctcca atgcatcctt actgtcaact atttttgctg tggtactgac
     6781 acctgtatgg atcgttgtat tacaaatgat tcatggcata ggtttatttt cataagaaat
     6841 ggaggaagaa agtatgagaa tcgcatgtta cggtgttcgt ccgaacgaga tagatttttt
     6901 tgaaaaattg aatatttacc attatgatct cagtctttat gaagaactgt tgacacatga
     6961 caatatcgtg tcagcaaagg accatgatgc tgtattgttg aggggaaatt gtatagcaga
     7021 tgagaccaat cttgcaaaaa tgcaggaata tgggattcgt tatgttttta cacggactgt
     7081 gggagtcaat catatcgatc tacaagcggc agctgatttt ggtatgacag tagcccgtgt
     7141 accatcgtat tcgccaaatg cgatagcaga gttatctttg acttttgcca tgatgctttt
     7201 gagaaatact gcgtatacga cgatccgaac atctttcaaa gattttcgag tggatgagca
     7261 aatgtttagt cgtgaaatca gaaattgcac agtagggatc ataggaacag gcaggatagg
     7321 gttgacggaa gctgttttgt ttaagggact aggagccaaa gtgatcggct acgatattca
     7381 tcagacagaa gctgctaaaa aagtattgac gttccactcg cttgaagagt tattagccaa
     7441 aagcgatatt gtcagtattc atgtacctca tatcccaggt gaaaacgatc aaatgatcaa
     7501 tgaaggattt ttagctcaaa tgaaaaaagg tagcatcttg attaatacag cccgtggaga
     7561 gcttcaagat aatgaagcta ttttaagagc tttgaaaagc aatcatttag ctggttttgc
     7621 aaccgatgtt tttgcaaatg aaacagaggt ttttttccga tccttcaagc catgggagac
     7681 gattccagac cctgctatcc agcaattagt agaaatgtac cctagagttc ttgtaacccc
     7741 gcatgtaggt tcaaatacag atgaggcatt atcaaatatg atttcgacaa gtttcgagaa
     7801 ctttcgtgaa ataatagaaa ctggaaaaac gaaaaatgag gtatctcttc ctaaagcccg
     7861 tcaattaaga taagaaaaca tggatattcg tcaataaaat gtttaaaaat agtccaagta
     7921 aacagttgtt acttctgtat acttggacta ttttgtatgg gataattcga tgactcaaca
     7981 gtaatctgtg agctttttcc gcccattctt tgtcaattca taacaggtta gcggctggcc
     8041 gatttccaaa tatttgattc tttcgttcaa tatatcttgt tctactaaaa aattcaaata
     8101 tttcttgacg gatatacgtg acagtgagct tttttgagcc agttcttttg ttgaaaaaat
     8161 agtatcttct tttagaataa atgcacttat tttttttaaa gtcagttttg acagaccttt
     8221 aggcaaatcg atgaatgatg gtatttcgtg aatgggagtg gtgtggttga atacttgatc
     8281 gatcaatttc tgatcggctt tttcagtcgt ttggattgtc tgagagaaag caagatactt
     8341 ttcgatcgcc agctgaaaac gttgcaaagt gaacggtttg atcaaataat ctaaggcacc
     8401 gtagcggatc acattcttta tggtttcgat atcatctgca gcagtgatca aaatcactgg
     8461 gatatcattt ccttgctgtc taagccaaga taaaaaatgg ataccacttt cgccaggcat
     8521 gtatacatca agcagcagca gatctgcgtt tttttgtctg aaatattttt ttccttcttc
     8581 tacatttctg aaactttcga ttacgttcat attagggatg ctttccacaa aagtacgatt
     8641 caattctgca accattggat catcttcgat tatcaggaca tttgtcatca ttttgatctt
     8701 ccttttttgg taattctaga tagacgattg taccgccatt tgtttgtttg gaaaattcta
     8761 aaaatccacc gtggcgtttg acgattgaat ggatgagatt caatccaaac ccacgattga
     8821 ttcccttcgt cgaaaaacct agcgtaaata ttttttctga gatttcttct gatatcccga
     8881 caccgccatc gcagacggtc acgatgaata cttcagcttc tgtgtcatag tttaaatgaa
     8941 tccggacctg tccgtttctt tcatgttttt gtagcacggc ttcttttgca ttatcaagta
     9001 agttccctaa cacttgcaaa agtgaatgga tggagtcatc catctccatt ttagggataa
     9061 aagaatcgct ttctaacagc atttcaacag attgttcttt ggcttcgttt attttgccta
     9121 ataaaaaacc ggcaattgca ggtactttga tcagacttga gacttggcca atctcttttt
     9181 tataattttg atttagctgc tggataaaat gggtgacttc ttcatacttc ttcaaatcaa
     9241 tcaaaccaga aatgacatga agtttattca tgaattcatg ggtttgagct ctcatggagt
     9301 ttatatattg tttcgtcccc ccaagctcct ccattaattt cattacttct gatcgatttc
     9361 gtaaagttgc gactgctcct aaaaaacggt cattgctaaa aagaggtgta acgctagaaa
     9421 tagtttctat cccatttaaa tacatggatt gagtccgaac ggtcgtttga tgctcaatcg
     9481 tctctttaaa caaaacgtca tatagattat cactgatttt tttcctactt cgaaatccaa
     9541 tagctgctct gtttggtaga aaatgttttt tgcaggtgta ttgatcaaga ggatagtttt
     9601 atcagaagag atggctaaga ttccttcatc tacttcattt tcgataatcg ttttttcact
     9661 taactgttct gctatttctt gtggttcaag tccaagcagt acttttttca ttttttgggc
     9721 aagaaaaacg gcgcctatga tgcctacgct aagtccaata atcaatgaaa aaacaatcat
     9781 tttttgtcca tctttgactt gattgtcaat cgtgttgttt gttaagccaa cacaaactgc
     9841 accaataatc tcttgatcat aataaatagg gacgaaatag cggaagccga accctaacat
     9901 tccttctttt tcagagaaaa attcttgtcc tccaatcact tttttcacat cttcatgact
     9961 tgagaaagga agaccgatgt aacttgggtc tgcgtgagaa tatcgaatgc cttcagggtc
    10021 catgacaacg ataaaatcta aattcgatgc aatcatgatt tcatttgtta ttccttgaat
    10081 cgcatgtgtc gggtcaccag tctgcagctt tttttggatg agtttatcat tggcaacgat
    10141 tttagcaatg ctagagattt tatctgacac actagatcgg ttcatcgaaa gaataaaatt
    10201 cctgatcaat atggcagaaa caatcaaaga aacgaatgtg gagaaacaaa cgacaaggat
    10261 gatcgtgttc tggagcttta atggtttttt cttaaaaaaa ggaaacatag gctacctcga
    10321 aagaatttat tgtgaaaatt ttaacatgaa aacaaaaatt taacaaattg tcaacgataa
    10381 acacaacctt cctataaaaa acaccactga gtcggtaaaa gaagctatac agaattttca
    10441 aacattggta tgatagacgt agaaatggaa ggaggcgtgt atatgatagg gggaatcgca
    10501 cttgagacga tttattggta tacattgctg atttgtgcag gattggcagt cctattgatt
    10561 atttttggag atgtattcga ttttgatggc ccgcttgatc cgatgcttat tatcccttgg
    10621 ttaactttca caagcttatt tggttttcta ggtgagcgtt taacagatca atcatcttta
    10681 tttttattta ttgctagtgg aggcatatcg atagtgttgg tatttttgct taatttctat
    10741 atattgatgc cgatgaaaca tgccgaatca acgatgtcgt cttctgaaaa aactctggaa
    10801 ggacaagtag ctgttgtgat cacaccgatc cctgtacgag gtatgggaga aattcagttg
    10861 aaaagtgtta ccggttccat tagtcgtcca gcttgttttt acagccccca agaacagaca
    10921 gctgagcggg gaacgaaagt attgatcatt gagatcaagg aaagaatctg ttatgtcatg
    10981 ccttatgaaa gcatgataaa agcttaaagg agcgtgtgtt aaatggaaat accagttgtt
    11041 tttatcgcga tcgcaatcgc agctttgatt ttactgatgt tgctgatcgt ctttgtagcg
    11101 aagtaccaga cagcaaaacc ggatgaagcg ttgatcatca gtggaagtta tttaggaagc
    11161 aaaaatgtgc acattgatga aagcaataac aaaatcaaaa ttgtgcgtgg aggtggcgca
    11221 ttcgtcttgc cagtttttca acgttcgaat cggatcagtc tattatcgag taaattggac
    11281 gtctccaccc ctgaagtgta taccgaacaa ggcgtaccgg tgatgtgtga tggtacttcg
    11341 atcatcaaaa tcggttcatc tgtagaagag attgctacag cagcagaaca gtttttaggc
    11401 aagacaagag aagaactgga aaatgaagca agagaagtat tagaaggaca cttgcgctcg
    11461 attcttggtt ctatgactgt agaagaaatc tatcaaaatc gcgataagtt cagtcaaagc
    11521 gtacaagaag tcgcttctgt agaccttgca aaaatggggt tgatcatcgt ttctttcact
    11581 atcaaagagg tcagagacaa aaacggttat cttgactctt tagggaaacc aagaatcgcc
    11641 caagtcaaaa gagatgcaga tatagcagaa gcggaagctc taaaagaaac gcgtatcaaa
    11701 aaagctcaag cagaaaaaga atcacaagca gctgaattac agcgacaaac agagatcgca
    11761 gaatcattaa aagaaaaaga actgaaatta gcaacctaca aacaagaaca agatgttgct
    11821 aaagcaaaag cagaccaagc ctacaattta gaaagtgccc gagcacaaca acaagtaatc
    11881 gaacaggaaa tgcagattaa aatcgtagaa cgccaaaaac aaatcgaact ggaagaaaaa
    11941 gaaatcacac gtcgtgaaaa gcaatatgat tcggaagtga agaaaaaagc agatgccgat
    12001 cggtacgcta aagaacaaga agcacaagct caaaaagtca aagaagtgac tgaagccgaa
    12061 gcagaacgat tccgtgtgga agcactggca gaagcggaag ccaataagac acgtttagct
    12121 ggacaagcag aagccgaagc agcactagca aaaggaaaag cagaagccga agcaaaacaa
    12181 aagatcgcca atgccttcaa ggaatatggc gaagcagctg tcttgagtat ggttatcgat
    12241 atgctaccgc aattgatgcg cgaagcagca cagccgctag gaaatatcga aaaaatctct
    12301 gttgtcgata caggaagcag ttcaggagaa accagtggag cgaaccgagt gacaaattat
    12361 gccacaaatt tgctttcttc tactcaagaa acactaaaag cgacaacagg acttgacttg
    12421 aaggaaatcg tagagaattt gtcgacacgt gggactacta gccaagtaaa tatgtatgaa
    12481 gcgccaaaag aggcagaaca aaactcgatg aaggaagaat aaagtagtac acttcaccgt
    12541 tcaatcaaaa atggaggaag actttttaaa gttcttctct ccattttttt attttattgc
    12601 aaaagaaaat tagtcctcgc tatttttctt ttgtttttgg atcttagctt ctatctcttg
    12661 taaatttgtt cttagcaatc gttctttctt ttggagttcg accaaatctt ctgatagttc
    12721 ttccttcttt tctgtttcag gaggacgaag aaagaaattg ataccgattg caatgaatgt
    12781 ccctataaaa gtatctagta cacgttggat agcatacaaa gcggattcgc cttgagggat
    12841 gctcaacgca atcagcaaca acgtagctat tgcggaaatg ataccggaat tattgttaat
    12901 accatctgac acgacgatca caataataac tagaatagga agcaagatca attctaccaa
    12961 aaaatcattg tgaaaatagc ttttgacaaa aaaataaatg atagctaatg taccacctaa
    13021 tgtattgccg atgatcctag actttccaaa agaaacggtt gtagtcaagt cctgacgcaa
    13081 agaaaaaaca gcagataatg cagcaatcat cgggattcct cgttgagtca aatgaaaaaa
    13141 caagatacat aaaaaaacag ctaaggcact tttgatcgta cgcataccta gtcgaaaacg
    13201 gccaaattgc atagagattc tcctttttgg tctttttact atcataccta aacttttgtc
    13261 tgatagctag tttttcatta ttcaagtata attaaaataa aaaaggggga agtaaatgcg
    13321 tataggaatg cgaacagtca aaacagtgat cagtgtcttc ttttcgatgg tcacagcggg
    13381 tagcttatcg ctgttgtatt ggccagctgc agggatcatt gcattattaa gtgtaggaaa
    13441 taccaagcgc tctacattgc gtacgggcat gaatcgaatc gcagccttta tattagctac
    13501 ggcaacagca ctgttcagtt tttttgctgt tgggtatact atttttggct tcagtctttt
    13561 tttattcctg ttcatcccgc ttgcctctcg cttcaaatta accgaaggga tagtagtaaa
    13621 ttcggttttg gtcactcatt atttagtgga ggaaaacatg tcttggcagc tgatcggcaa
    13681 tgaagcggga ttaatggcga ttggattagt atttgctttg cttgcaaatg tttacatgcc
    13741 agatcgtacg aagcagctga aggaaaacca aatacagatc gaaaaagaat ttcgaaatat
    13801 tttgagacaa atggcagaat ttcttttatc agaaaataaa gaagatgtgc agatcaaatg
    13861 cgaacattta ctgacattca ttcgcgaaag ccaagaagat gccagagaac atcaagaaaa
    13921 ctactggctc agacagccgc tatactacga aacttacttc tctatgagaa gagcacaggc
    13981 caatgtgatt aaagacatgc tggaaaatct agagagaatc cagcaaccag cttattatgg
    14041 aaaacatatc tatggtttat tgatttatac tgcagaaaca ttttcagagt caaatgatgg
    14101 cagacaaatt ttaacacgta ttgaagaagt ttatgtattg tatcggcaaa tgcctctgcc
    14161 gactagtcgc cctgaattcg aggatcgagc agaattgttc cagtttttgc agtcttttaa
    14221 aagttttatt gaaatcaagg cagaattctc ccaacaaaaa atccaaaaat agaggttgtg
    14281 aattcagcta atattcaggt caaaaagctg atttcacaac ctcttcttta aacggtgttc
    14341 tcctgtcaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg
    14401 aaattgctcc ttgtgactcg gagctgaacg gtaggctcac aacctctttc ttaaacggtg
    14461 ttcaaaagct ttcctctgcg gtaataagtc cagccaaacg aaaaatagga ggagctattt
    14521 ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctt ttaaaattac
    14581 atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt tgtcaagaag
    14641 agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat ttcgtaattc
    14701 attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt aaataagatc
    14761 tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc aaaggcaacg
    14821 ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag ttcattttct
    14881 ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg aaccattgta
    14941 gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac agcatacgta
    15001 ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt tttagggtct
    15061 ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag ttttcctgta
    15121 tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat cgcttctgct
    15181 aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt tttattcatc
    15241 gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa gatatggtcg
    15301 atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc gtctaacatt
    15361 tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt ttccgctttt
    15421 tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa gctttcggca
    15481 aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt catctgaaaa
    15541 cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa aaatcacgaa
    15601 gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt taagaggtgc
    15661 tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc atcaagtata
    15721 tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa attttttaga
    15781 tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg agaaaaatct
    15841 gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat ggatgctaac
    15901 gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg ccgttgcaga
    15961 gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag cagcaaaagt
    16021 cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact tgaaagtaag
    16081 tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc cagcagatgt
    16141 tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg aaaatctgga
    16201 agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg atttttcaag
    16261 tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg ggctatgtta
    16321 tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa cgaacaagca
    16381 ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag gtaaaggaca
    16441 aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac gattggaagc
    16501 tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct actcaattgg
    16561 catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat gaagcatttg
    16621 ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac cggtctgtat
    16681 catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt caaggtgtat
    16741 cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga tacagcgaac
    16801 accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt tatccttcta
    16861 ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca gataatgctg
    16921 cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact aagatcacat
    16981 atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc accaaacacc
    17041 aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg atcatggcga
    17101 aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg gttgccggtg
    17161 tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct ccttttactg
    17221 ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg cttgttccgc
    17281 atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta ggacaagcta
    17341 tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat cttttcggga
    17401 tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt gttaacgaag
    17461 aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag tccatggatg
    17521 accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat gaaaaagtga
    17581 ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt tatgcgacag
    17641 accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg aatcagtatg
    17701 accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag aaatcatgac
    17761 ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa gcgggatcaa
    17821 gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga tcatacgtaa
    17881 tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag cgattactaa
    17941 aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg gtttgacaca
    18001 aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt taggggtcgt
    18061 tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag ttgatattcc
    18121 agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag cagctggtat
    18181 ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc attttcgtaa
    18241 aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa gtattgctga
    18301 gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg gagagctttt
    18361 tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat aacaggtgag
    18421 aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac tccagtacta
    18481 tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta atgtacataa
    18541 gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct tgatttattg
    18601 aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg acaatttagt
    18661 agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa gttgccatag
    18721 tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt actagttatt
    18781 tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct atgtcaagga
    18841 tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca tcattgaagc
    18901 aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct tgaaagtatt
    18961 gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc ccttgaaaaa
    19021 gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata acaatggaag
    19081 tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt gttatgttat
    19141 tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac atggcttggt
    19201 ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat gaagaaaaaa
    19261 atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac acaaaattag
    19321 gtacactagc agcatcagat gatatctggg acattttcca actgaacaat caatttcctc
    19381 aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata attgatattt
    19441 ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta gttggactgt
    19501 ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa caattaaata
    19561 tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc tatcaaatca
    19621 ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct ggctgtatta
    19681 ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat ctatctaaca
    19741 gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt aaaaatctgg
    19801 tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg gatctgtctt
    19861 ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac cagttgacag
    19921 aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca cctcgcagaa
    19981 aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt atggcaaaaa
    20041 gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac agtgaagaag
    20101 ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt cgagaacagc
    20161 tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa atgatttccg
    20221 atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa attcaagacc
    20281 aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa ggaaggcatg
    20341 aaaaaagaaa aactaaaacg gttcttggat caagacaatg tggtcggtta cgtatttgct
    20401 tctccattca tcctaggatt tctattgata acagtgatac cgatgatctt gtcactttat
    20461 tattcgttca ccgattatcg tttgggaaat ccagtctcat ggatcggctt ggataattat
    20521 ataaagatgc tgagtgatgc ccgttttttg aactccatcc gagtcacttt tctctatgta
    20581 tttgcatctg tacctgtgaa actgatcttt ggtctattga ttgcttatct tttaacgagg
    20641 aatatcaaag gaacgacttt ctttcgttct ttgtattatg tgcctagttt gatcggtggc
    20701 agtatttcta tcgctctagt ttggcgggag ttgttttcta aaaacgggtt agtgaatatt
    20761 ttcctggaac gattaggcgc acctgcgctt tcctggttta gcgatcaaaa actggtcatg
    20821 atccctcttg ttttgatgtc tgcttggcaa ttcggttcgg caatgatcat ctttgctgca
    20881 ggtatcaaag agatcccagg ctcgtattat gaagcggcag agatcgatgg agcgaacaaa
    20941 tggcagacgt tttacaagat cacgttacct cttctttcgc cgatcatttt gtataacttg
    21001 gttatgcaga caatcgcggc gtttatgact tttacgcagg catttatcat tacccaaggt
    21061 ggtccgaatg atgccacgaa cttttatgct ctttatgtgt ataaccaagc tttttctttt
    21121 aattctatgg ggtatgctag tgctatttcg tggatcatgc tgttgattat gggagctgtc
    21181 acctttctga ttttcaaatc atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga
    21241 aatgaataat acagtaacga aatggtttca atacggtttt attttgctat taggtctagt
    21301 catgatttat ccggtcttat ggatgatcgc aggttctttt aaaacggatc aagagattat
    21361 gagtggttcg ctgaatttga ttccagagac ttttcaatgg gaaaattacg caaaaggatg
    21421 ggcggggttt gctggaattt cattttttac tttctttagg aattcgtttg tcatctctgc
    21481 agtttcgacg atagggacag ttctgagctc gacttgtatt gcttacgcat tatcgagaat
    21541 cgatttcaaa ggaaaaagat tctggttcgt cgtcatgtta gtaacgatga tgattcctgc
    21601 acaagtgatt ttgattccgc agtttattat ttacaatcgg ctgaatttgg ttgggacgta
    21661 tgtgccgctg attttaccac atttttttgg acaagcattt ttcatttacc agatcatgca
    21721 attcatggtc agcatcccca aagagttaga tgaatcagcg attattgacg gatgcagcaa
    21781 gtatagtgtc tttaccaaaa ttatttttcc tttgctgaaa ccttcgatca tcacgacgat
    21841 catcatccag ttttattgga aatgggacga cttcatggga ccattgattt atttaaacaa
    21901 gccgagatct tacacggtat cgattgccat caaattattt gcagacgctg gatctagtac
    21961 gacttacgcg agtatgtttg cgatgtctac gttgtcgctg ctaccggttt ttctgatttt
    22021 cttattcttc aataaatatc ttgtccaggg gatcagtaca agcggtctga agggctgaat
    22081 atgaaaaagc caatagaaga gatgtttctt tacaagctca actactatgt ctgtcggatt
    22141 tttttaacca gtcttatcag taatgctctt ttcgctttag ttctaggatg tttgttcact
    22201 ttaggattag tgctgtctgc tcgtgttgtc tatctgattt attttttgat gatgtatcct
    22261 tcttattatc tatctgtcca ttacttggca gaaaaaaagg tatgttggag gaaagggaaa
    22321 atcaaacgga tgtgtttcat cattacgttg tttcagctgt ttgtcattat ttttgcagtt
    22381 gattggtatt tattagtggg aaagattgga gcttggtatc tgattccttt tctattgtgt
    22441 ctgttgcttt ttgtatccca tagttttttt agtacgctaa ttgatatctg caaagaagat
    22501 aaagcagata gtcttgttta tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc
    22561 aaggggtttc tgtggtattt atgccttttg tatggactgt tctggtgtat acagcctgat
    22621 tttttactca ctgcattact gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa
    22681 gaaaaggaga atgactttaa atgaagaaaa aatatgtaca agtcggactt ggtgggagag
    22741 cgcgtttttt ttatgaggca attgcctcta cgtatcatca aacttgtgaa ctagtcggtt
    22801 tttgtgatgt atctcaaaca aggatggatt atgcaaatcg gatacttgtg aacaagtatg
    22861 gacagaaaaa agtaaagaca tgtatgccat cacaatttga gcaactactg gatgacgtac
    22921 ggccggctga agtcattatt acgtcagtag atcgaacgca tcatgattat atcattcgtg
    22981 caatgaaaaa aggatatgat gttatttgcg aaaaacctct gactatcgat gaggaaaaag
    23041 ctcaagaaat aataacggtc caaaaacaga cgggaagaag cctgcgagtc acttttaatt
    23101 atcgttatgc accgtatcac acgaagataa aggaactgat agaaagtgat gtgattggtg
    23161 atgtgttttc tatccacttc gaatggctgt taaatacgca acacggggca gattatttca
    23221 gaagatggca tcgaaataag cacaatagcg gcgggcttct tgttcataaa tctacccatc
    23281 attttgatct agtgaatttt tggttgaaca gccgtcccca aaaggtattt gcattcggtg
    23341 gattaaactt ttatgggatg cataatgcgg agaaacgcgg agtagaaata ttttacagtc
    23401 gatgctatgg aagcgaacta gctaaagctg atccatttgc tatagacctg tcagccaatg
    23461 aggaattgcg agaaatgtat ttagaagcag aaaaagacag tggatatatt cgtgaccgca
    23521 gtgtattttc cgatgatatc tctattgaag atacactagg cgtaacggtc aaatatgaaa
    23581 ctggtgcgat tttgacgtat tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt
    23641 ctttcaatgg cagcaaagga cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg
    23701 caggtggaga taaaaaggat gaaggagcag ccgttcttca gtccatccat gtctatccga
    23761 tgttcggtga gccatatgag gtaatcgtcg aacaagaagc gggcggacat ggcggaggag
    23821 atccgcgttt attagatgat ctatttggtg aagaaaaaga agaagatccg tggaatcggg
    23881 cagcaactca cgtagatggt attttgtcga ttttgacagg aatcgcagca aatcattcta
    23941 ttgcttcagg aaaagcagta gtgattgacg agcttgtcag cttaaaagaa taagaataga
    24001 tttacggaaa ttacttcccg tagtttataa aatcttgcct tattgccgat aggctgtctt
    24061 ttgaacacta tttattcgga aaaaggggct atgacatgct ttttgtcatg ccttcttttt
    24121 atgtgggctg cttttttaga taggagaaaa taaaaataac tatttaaacc cgaacaaaaa
    24181 ggaataatgc attaaaaatc attagatttt acaaaaaaat aagtaaacca cgttgtcagt
    24241 ttcagaaagt agtgttagac tgttttcaat atttgaaaca acatttttta ttcaaatatg
    24301 gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga atttacacta ttattagttt
    24361 tacataccta cttatatatc gtcataatat ggatgacagt ttctagccag taccgtaaat
    24421 gctggactat aagtaaaaga ttggctattt cacccttctt ttcttatgat aaagtggagg
    24481 gtgtttttct ttaacagaaa catagtaggt ttttgcacaa aagcggttat ttcggtatta
    24541 ataaatgata aaggagcaaa taaagtgaaa gaattagttg aacgtattca aaaagacgga
    24601 cgtatattag gcgagggcgt attaaaagtc gacagcttcg tcactcacca agtagaccct
    24661 gtattgatgg aacaaatggg aaaacggttt gcagaagtat ttaaagaaca aggaatcacg
    24721 aaagttgtga ccattgaagc ttctgggatc gcaccagctt tatttgctgc aagagaatta
    24781 gatgttccaa tgatcttcgc tagaaaagca aaaagtttga cgatggatga agaattattg
    24841 acagcttctg tttattcttt tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa
    24901 tttttatctt cagaagacaa agtattgatt atcgacgatt tcttggcaaa cggacaagcg
    24961 gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc aagtagaagg aatcgggatc
    25021 gtcatcgaaa agtctttcca agatggacgt cagttacttg aagaaatggg attgagagtc
    25081 gtttctcttg cgcgtattgc ttccttgacg aatggacaag ttgagtttct tgaggaggac
    25141 gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc
    25201 tgctagcgat gtatgcagga gcagtagcgg tgcctctttt gatcggtaca ggactaggct
    25261 ttaatcaaga acagatgact tatttgattt caattgatat ctttatgtgt ggattagcga
    25321 cattattgca attgacagtt aaccgatttt tcggaattgg cttgcctgtc gttttaggat
    25381 gtgcgatcca agcagtagct ccactgatct tgatcggaac caatgaagga gtaggagcta
    25441 tctacggatc tattatcgct tctgggatct ttgttgtgtt agtttctggt gtcttttcta
    25501 aaattaaaaa actattccct cctatagtaa caggaacggt tatcaccgtt attggtttga
    25561 ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa tgcaacagca gctgattttg
    25621 gtgacaaaac aaatctgcta cttgcatttg tgacgattct cctaattgta ggcgtgcaaa
    25681 tgctggcaaa agggttcatt cgttcaatcg cggtattgat tggtttagtc ggaggaagca
    25741 tcttagctgc tttcatggga atgattgatc tgagcgcgat tggagatgcg ccgttgttcc
    25801 atgttccaca acccttttat tttggtaaac caactttcga cgtatggtcg attttactaa
    25861 tgatcatcat ttccatggtc agcatggtag aatcaacagg tgtatatttt gcacttggtg
    25921 acattactgg gaaaaatgtt ggagaagaag aattgaaaaa gggatatcgt gcagaaggac
    25981 ttgccgttat cttaggcggt attttcaata cattccctta tactggcttt tctcaaaatg
    26041 tcggattggt tcaactttcg ggaatcaaaa caagaaaacc catctatttc tctgcattct
    26101 tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc
    26161 cggtacttgg tggaggaatg ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta
    26221 tgctgttgga agttgatttc agcaatgata aaaatctatt gattgttgct gtttctgttg
    26281 gatttgggct gggtttcaat gttacgccga cattattcag tcaaatgccg caaacattgc
    26341 aaatgttcac tggaaatggc atcgtaatga gtagcattac ggctatcata ttgaatttga
    26401 tctttaatgg attgaaaaaa gatgaagaaa tattggaaga agccttcaag aagtaataga
    26461 gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg ttagagaaac aaacggaaaa
    26521 atagttcctc gtattttgcc gtttgtttga gctaataccg agggtcagct gattgaacac
    26581 cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac attccttttt ttcatcttta
    26641 ataaacacga acaataaaat gtttttcttt gttaacattc gaaatttctt tgacatcaaa
    26701 aaaagtgatt gttaaaatat gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa
    26761 atctctgtga ttatggggag tacttcagat tgggaaacaa tgaaagaagc atgtcaaata
    26821 ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat
    26881 ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga
    26941 gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt gccggttatc
    27001 ggtgtaccgg tccaatcacg aacattaaat ggtttggatt cgcttctttc gattgtccaa
    27061 atgcctggag gagtacctgt agctactaca gcaatcggaa aagcgggagc aattaatgct
    27121 ggactacttg cgatacagat gctttccatg tatgatttag aattggaggc taaattggct
    27181 gaaagacgtt caatgttggc taaaactgtt atagaaagta gtgatcaact tggttaaacc
    27241 tttgttgcct ggttcaacga tcgggatcgt cggtggcggt caattaggtc gtatgatggc
    27301 gattagtgca aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc
    27361 agccgcacaa gttgcagatt ggcacattga aagtgtctat gatgacacct ttgcgctgaa
    27421 agagcttgcc agaagaacag atgtcattac ctatgaattc gaaaacgtca gtgtagaggc
    27481 gttaaatgcg attttaccca tgtcatttat tcctcaaggg acagacttgc ttgcgattac
    27541 gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat aacatcgtga ttgctcctta
    27601 tgcaacgatc gtcagtccga cggatatcca agatgcgatt gacgggatcg gctatccttg
    27661 tgttttgaaa accactcgag gcggctatga tggcaagggg cagtatgttt tgaaaagtcg
    27721 ggcggatcta gctcctgcca tggacctttt aagagaagga acctgtgttt tagaagcatg
    27781 gatcccgttt gaaaaagaga tttcgatcat ggtggcagga aatggtcaag gagatttcac
    27841 gacttttccg gtcgtggaaa acatccacca caacaatatt cttcatgaaa cgattgctcc
    27901 agccgcgatt gatcaggatg tgatcgagga ggctgagcgt attgcccgtg tgattgcaga
    27961 agcagtgagc ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa caggcgggct
    28021 atatgtgaat gaattagctc ctcgtcctca caattctgga cattattcta ttgaagcttg
    28081 ttctatgagt cagttcgatg cccatattcg aggtatttgt ggttggccat taggagaaaa
    28141 agtacgtttg ataagcaatg cagtaatggt caatgttttg ggagaacaac tgctggaaag
    28201 ctatcgcttg attgagaata agccagactg gcaattccat ttctatggaa aaaaacaagc
    28261 aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca gaagacatcc agcaaacgct
    28321 ggaagaaatc aaacaaacga atatttggga ttagaggaga aaaaacatgt tagaacgtta
    28381 tacacgccct gaaatgggga atatctggac ggaagagaac aaataccgtg cgtggctcga
    28441 agtcgagatt ttagcagatg aagcttgggc agaacttggt gaaatcccgc aagaagatgt
    28501 gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg atcttggaaa tcgaaaaatt
    28561 aacacgccat gatgtcgttg cgtttacacg agcggtttct gaaacattgg gagaagaaag
    28621 aaaatgggtc cattatggtc taacaagtac ggatgtcgta gatactgctt atggctatct
    28681 catcaagcaa gcaaatgata ttttacggga agacttgaaa cgttttacgg aaatcattgg
    28741 cgaaaaagca aaagaacaca aatatacagt gatgatgggt cggacacacg gtgtccatgc
    28801 tgaaccaact acttttggat tgaaacttgc cacatggtat tcagaaatga aacggaacat
    28861 cgaacgattt gaacatgcgg caaaaggagt agaagcagga aaaatctcag gagcagtggg
    28921 aacgttcgca aatatcccgc catttattga agagtatgta tgtaaacaat tggggatccg
    28981 tccacaagaa atctctactc aagttctccc tcgggatcta catgctgaat atttctcagc
    29041 gatggcgctt attgctacaa gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa
    29101 atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt cttctgctat
    29161 gccgcacaaa aggaatccga tcggttcaga aaatatggct ggtctagcca gagtggtgcg
    29221 cggacacatg gtcactgcct atgaaaatgt cacattatgg catgaaagag atatttctca
    29281 ttcttcagca gaacgaatca tcttgcctga taccacgatt ttattgaatt acatgttaaa
    29341 tcgttttggc aatatcgtta aaaatctaac ggtctttcct gaaaacatga agcggaatat
    29401 gtctgcaaca tttggtttga tttatagcca gcgcgttatg ctgaaattga tcgataaggg
    29461 aatgacaaga gaagaagctt atgatcttgt tcagccaaaa actgcgtacg cttgggacaa
    29521 ccaagtgcag ttccgcccgc tgttagaaga agatccaacc atcacatcgg tattatctaa
    29581 agaagaaata gacgatgcct ttgattatca tcatcatttg aaaaacgttg acgtgatatt
    29641 caaacgagta ggtttggaat aaagaagagt gagttgcaaa attcaattaa aatagaatag
    29701 caaaaataac caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata
    29761 ttttcttatc aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt
    29821 tacatacaaa aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg
    29881 gttatggaga tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa
    29941 taagatctgg atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga
    30001 aagttcgcct ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg
    30061 cttggttgct cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata
    30121 aacgtctttg atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc
    30181 tgataaaaaa agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc
    30241 cattatccaa atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt
    30301 gttgatggta taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct
    30361 taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa
    30421 acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag
    30481 aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac
    30541 ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag
    30601 ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact
    30661 caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa
    30721 aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca
    30781 aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat
    30841 aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt cttacaactt
    30901 gcaaaaccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg
    30961 accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg
    31021 tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat
    31081 gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc
    31141 ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta
    31201 tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg
    31261 aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag
    31321 gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa
    31381 cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa
    31441 aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac
    31501 gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat
    31561 gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt
    31621 gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt
    31681 tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa
    31741 gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact
    31801 gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata
    31861 atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa
    31921 gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg
    31981 gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca
    32041 gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt
    32101 ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa
    32161 gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg
    32221 atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt
    32281 atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact
    32341 agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat
    32401 ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat
    32461 gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt
    32521 ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat
    32581 ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact
    32641 gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg
    32701 tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat
    32761 tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc
    32821 tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct
    32881 tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct
    32941 ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct
    33001 tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg
    33061 atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa
    33121 tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact
    33181 atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca
    33241 aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa
    33301 ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct
    33361 ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac
    33421 gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct
    33481 gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc
    33541 tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat
    33601 attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct
    33661 gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt
    33721 gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt
    33781 caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat
    33841 atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt
    33901 gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc
    33961 cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa
    34021 aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca
    34081 gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa
    34141 ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga
    34201 ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata
    34261 atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc
    34321 tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct
    34381 tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc
    34441 agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc
    34501 atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa
    34561 caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca
    34621 atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta
    34681 acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt
    34741 ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt
    34801 taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga
    34861 cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac
    34921 aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg
    34981 cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc
    35041 agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt
    35101 ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg
    35161 gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt
    35221 cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat
    35281 tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc
    35341 aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta
    35401 tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag
    35461 aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt
    35521 tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg
    35581 aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg
    35641 aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg
    35701 gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa
    35761 aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca
    35821 tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag
    35881 catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt
    35941 tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg
    36001 gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc
    36061 gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac
    36121 tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca
    36181 gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt
    36241 atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct
    36301 caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc
    36361 gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt
    36421 tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca
    36481 acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct
    36541 gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg
    36601 gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa
    36661 tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc
    36721 ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa
    36781 aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga
    36841 gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag
    36901 ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc
    36961 ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac
    37021 ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa
    37081 aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga
    37141 aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt
    37201 tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt
    37261 atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac
    37321 aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc
    37381 cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat
    37441 cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt
    37501 agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct
    37561 ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca
    37621 tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat
    37681 ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga
    37741 aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg
    37801 tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc
    37861 atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt
    37921 cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc
    37981 tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga
    38041 acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc
    38101 cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc
    38161 ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc
    38221 aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt
    38281 agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt
    38341 tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat
    38401 catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat
    38461 ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc
    38521 agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg
    38581 gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg
    38641 gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt
    38701 aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc
    38761 gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc
    38821 ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa
    38881 agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt
    38941 tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg
    39001 tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta
    39061 ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga
    39121 gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt
    39181 tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa
    39241 aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca
    39301 attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa
    39361 agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc
    39421 agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca
    39481 aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt
    39541 ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt
    39601 catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat
    39661 gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga
    39721 agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt
    39781 atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt
    39841 cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat
    39901 cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt
    39961 aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc
    40021 agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa
    40081 actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga
    40141 agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg
    40201 tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt
    40261 ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca
    40321 taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa
    40381 ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca
    40441 acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt
    40501 ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag
    40561 caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag
    40621 gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc
    40681 gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac
    40741 cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg
    40801 gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat
    40861 tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc
    40921 atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc
    40981 gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg
    41041 tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta
    41101 aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata
    41161 tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag
    41221 aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac
    41281 cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga
    41341 aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat
    41401 tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct
    41461 tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa
    41521 ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct
    41581 aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa
    41641 atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata
    41701 tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat
    41761 ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc
    41821 gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga
    41881 accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag
    41941 acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg
    42001 atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa
    42061 aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt
    42121 tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat
    42181 tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt
    42241 tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca
    42301 agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt
    42361 tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct
    42421 gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg
    42481 aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc
    42541 aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc
    42601 tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc
    42661 ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg
    42721 tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa
    42781 aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag
    42841 tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga
    42901 taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct
    42961 tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat
    43021 catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc
    43081 atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa
    43141 atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat
    43201 gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc atgcggtaat
    43261 acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa
    43321 tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt
    43381 atagtcaagc atatggccgt atcttatttt taagatgact agataatcat tgatatggct
    43441 aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc
    43501 ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat
    43561 aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac
    43621 ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc
    43681 ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt
    43741 taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat
    43801 cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt
    43861 actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt
    43921 ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag
    43981 ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc
    44041 caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga
    44101 tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc
    44161 attcctaagt tcttctagga tattttcctc attgaactca tctacggtta cttggccaga
    44221 aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc
    44281 tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat
    44341 gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat
    44401 ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga
    44461 tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg
    44521 tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga
    44581 catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat
    44641 cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt
    44701 ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt
    44761 cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa
    44821 aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt
    44881 tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg
    44941 gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg
    45001 tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa
    45061 tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag
    45121 ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag
    45181 taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt
    45241 cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga
    45301 taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa
    45361 cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga
    45421 ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt
    45481 gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa
    45541 aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa
    45601 atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg
    45661 aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt
    45721 gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg
    45781 gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa
    45841 catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa
    45901 agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt
    45961 gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga
    46021 tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc
    46081 gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg
    46141 attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa
    46201 caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg
    46261 tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga
    46321 tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac
    46381 attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa
    46441 aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac
    46501 agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac
    46561 acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt
    46621 tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc
    46681 aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta
    46741 taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc
    46801 agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa
    46861 ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat
    46921 gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg
    46981 tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa
    47041 gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt
    47101 ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg
    47161 attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct
    47221 atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata
    47281 gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg
    47341 aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta
    47401 acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc
    47461 attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat
    47521 attattcgcg ggatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat
    47581 attggagaag tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag
    47641 cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct
    47701 ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga
    47761 agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct
    47821 tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat
    47881 ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa
    47941 ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt
    48001 ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa
    48061 gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc
    48121 cgtgggatag atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca
    48181 aatggcaaag ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat
    48241 cgaatctata cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat
    48301 caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa
    48361 aaatcttacg gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt
    48421 tggtcgctac agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac
    48481 ggatggtatt ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat
    48541 attagtacta gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt
    48601 agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat
    48661 gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca
    48721 aggtttttgg gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct
    48781 gatgtttcgt ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc
    48841 tgttttccaa aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca
    48901 cttcttgaca ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg
    48961 aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt
    49021 aaaggcggta tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca
    49081 agggtatccg attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac
    49141 aaataaaaca gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga
    49201 actatcaggg atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat
    49261 ggcagcttta gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg
    49321 tacatcgatg gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg
    49381 cggcattgga acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa
    49441 tggcatgtcg attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt
    49501 gcttttagcc gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc
    49561 cggttattca gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag
    49621 cgaacattca ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt
    49681 ataaattctc tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct
    49741 aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg
    49801 ataatgtaat gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg
    49861 taaatagccg ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc
    49921 cagtagtgcc tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg
    49981 aaaatggtta tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca
    50041 aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat
    50101 attcctttat agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca
    50161 tcgcgcatgg acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc
    50221 ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa
    50281 atgcgggcgc agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa
    50341 tcaagacagg ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag
    50401 cagctcgcaa gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat
    50461 ctgtccgttt tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat
    50521 ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt
    50581 cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca
    50641 aaggatcttt gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt
    50701 atgctggagg aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt
    50761 cgatttttaa tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag
    50821 ttgtcctgca ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt
    50881 ggctggaatt attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg
    50941 tgataagatt tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt
    51001 aaattcggta aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa
    51061 agtagagaaa aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat
    51121 cgaaaacgga aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact
    51181 ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt
    51241 actaacgagc aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc
    51301 tgttccaaga atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta
    51361 tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc
    51421 tagcgagatg ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga
    51481 aatcttagcg gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat
    51541 caaacggtat ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac
    51601 tggtggcggt cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg
    51661 gatagctgta gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac
    51721 gcgtttattt gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc
    51781 gatccataaa aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg
    51841 aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct
    51901 tccaattatt gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct
    51961 tcctatacga acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt
    52021 aggaaaaaca acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc
    52081 tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa
    52141 tgtatttaat gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa
    52201 aagccgactg ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt
    52261 aggtggttac tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga
    52321 aaaagtagta gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa
    52381 tggaatcaga atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa
    52441 gatgaaaatt ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa
    52501 tataacagct tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata
    52561 taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa
    52621 atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt
    52681 ttgattagaa aatagaaaaa atgcagatga aaaataaaac gctttcctta ttgtgatttt
    52741 attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg
    52801 cttttagctt gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt
    52861 gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga
    52921 ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg
    52981 aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca
    53041 aagatttagt acctgattta tccgatgatg aactcgttga attaatgact catatggtat
    53101 ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt
    53161 ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag
    53221 aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt
    53281 tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat
    53341 tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg
    53401 cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg
    53461 gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg
    53521 ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg
    53581 cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg
    53641 gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg
    53701 gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg
    53761 gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc
    53821 cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag
    53881 aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag
    53941 taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa
    54001 tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac
    54061 aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt
    54121 tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca
    54181 ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg
    54241 attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg
    54301 ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat
    54361 gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac
    54421 accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt
    54481 agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa
    54541 cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc
    54601 ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt
    54661 atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc
    54721 aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga
    54781 aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg
    54841 acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt
    54901 agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga
    54961 aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt
    55021 ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg
    55081 tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa
    55141 tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc
    55201 tgttacagga acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga
    55261 tgtattagta gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc
    55321 cgcagaatcc caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc
    55381 tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc
    55441 agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac
    55501 attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac
    55561 agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga
    55621 aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc
    55681 aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa
    55741 acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc
    55801 acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt
    55861 ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa
    55921 agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa
    55981 cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc
    56041 aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc
    56101 taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact
    56161 tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt
    56221 gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg
    56281 tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa
    56341 aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa
    56401 actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg
    56461 tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac
    56521 gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa
    56581 actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa
    56641 taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa
    56701 tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg
    56761 gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag
    56821 agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg
    56881 caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg
    56941 tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga
    57001 cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt
    57061 tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt
    57121 tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg
    57181 gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg
    57241 tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag
    57301 cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag
    57361 taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg
    57421 cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag
    57481 aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg
    57541 atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata
    57601 aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg
    57661 cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa
    57721 aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt
    57781 ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat
    57841 tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc
    57901 tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa
    57961 gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg
    58021 ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag
    58081 cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat
    58141 cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga
    58201 caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga
    58261 gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt
    58321 cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga
    58381 agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa
    58441 acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca
    58501 agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg
    58561 attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa
    58621 acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac
    58681 atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca
    58741 gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct
    58801 attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt
    58861 tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt
    58921 tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat
    58981 aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat
    59041 ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt
    59101 gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg
    59161 gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt
    59221 ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat
    59281 gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa
    59341 aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat
    59401 ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac
    59461 caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta
    59521 ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata
    59581 cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag
    59641 aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa
    59701 acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa
    59761 tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt
    59821 ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg
    59881 acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat
    59941 taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta
    60001 cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata
    60061 caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag
    60121 tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag
    60181 gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat
    60241 tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa
    60301 aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta
    60361 ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa
    60421 tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc
    60481 gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac
    60541 caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct
    60601 tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg
    60661 aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag
    60721 aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata
    60781 tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga
    60841 ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt
    60901 ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg
    60961 tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta
    61021 cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc
    61081 gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg
    61141 ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt
    61201 tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa
    61261 aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa
    61321 aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac
    61381 tcttttctgt ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag
    61441 acgtttaaaa gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa
    61501 tgtttcacga aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga
    61561 aaggagcggg aagatgaacg acttattaaa acaatacttt ggttatgatg agtttagacc
    61621 tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc
    61681 taccggaagt gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac
    61741 tgtggttgtg tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt
    61801 aggtataccc gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt
    61861 ccaagaaatc gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat
    61921 gccggatttt atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc
    61981 acattgtatc tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca
    62041 gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca
    62101 agtagctgct gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg
    62161 ttttgaacga gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct
    62221 aatcgaatat ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa
    62281 agaagtagac cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca
    62341 tggtggtcta aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt
    62401 gcagctgatt gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt
    62461 tgttatccat tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc
    62521 gggaagagat ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt
    62581 ccaaaagttt tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga
    62641 aaaattaaaa gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct
    62701 aaactatttt ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag
    62761 agaaatagta gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg
    62821 tgagaactat ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct
    62881 gcaagcttta ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa
    62941 agaaacgatg cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg
    63001 tgagtatcct gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc
    63061 tgtttatcga aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact
    63121 atttgaagtg ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta
    63181 tgtcgtattt tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt
    63241 ggagatgctt cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt
    63301 tttatctcgg ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg
    63361 taaaaatgaa taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat
    63421 atgttataat taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa
    63481 cttgcgatcc aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa
    63541 aacaacagtc tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg
    63601 tacggctttt cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag
    63661 ggccagctga tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac
    63721 caagaaattt atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga
    63781 aaaaaaggca tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg
    63841 gattgatcat ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt
    63901 ccgcatcagt cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac
    63961 tttataaaat gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag
    64021 ttttgacgat tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag
    64081 gttggctgca aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta
    64141 tctggtacct gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg
    64201 ggactgtgaa acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc
    64261 ccgattttgg taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg
    64321 gagtcaatta tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta
    64381 cgatctggct gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct
    64441 ataatcgatt tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac
    64501 tagtcaacgg ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca
    64561 gtatccagaa aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag
    64621 tggaagaact gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg
    64681 tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg
    64741 ggatcggcgc aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta
    64801 ttcctttgac agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc
    64861 tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag
    64921 gtttataata aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg
    64981 ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga
    65041 atctatgcag aagaagatga atattccgta caccgtttca aagcggatga agcatattta
    65101 gtaggacaag gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc
    65161 gcgaaagaat gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg
    65221 aattttgcgc aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat
    65281 ttagatattt ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct
    65341 tcgattccag gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa
    65401 caatacggtt atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt
    65461 gtagctcacg atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa
    65521 gcagcctttg gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc
    65581 gaagtacaga ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt
    65641 tctgttcagc gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa
    65701 cagcaacgtc aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc
    65761 aacgcaggta cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac
    65821 cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca
    65881 acgcaactgt tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa
    65941 gaaggaatta aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg
    66001 aataactttt taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc
    66061 gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca
    66121 ttgctagtaa aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa
    66181 cgttgcttga aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat
    66241 gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca
    66301 gcaacattat ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc
    66361 ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa
    66421 gaaccacgta tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta
    66481 ttagatgcga agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg
    66541 ttgacggata cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca
    66601 aaggatttca agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt
    66661 gagatgtggg ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg
    66721 caacggttga gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga
    66781 ggttcaaatg ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa
    66841 gaagcagcag cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca
    66901 caaatggaaa aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt
    66961 tgctatactg gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa
    67021 gacatggcaa aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct
    67081 gggctgctta aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat
    67141 ctgccaatcc atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct
    67201 gcttcaaaag caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact
    67261 agtcagccta gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa
    67321 atcacgattg aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac
    67381 aaaccgtttg aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca
    67441 ggaggacagt attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg
    67501 gatgaaatca aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta
    67561 actccatcat ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca
    67621 gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc
    67681 ttccaaggag aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt
    67741 cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc
    67801 aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta
    67861 ttaagctatt tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt
    67921 ggcgatgtta cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca
    67981 atcaatgtac aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa
    68041 cccgatatcg aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc
    68101 actatcaaag ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca
    68161 aaccgagaac aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa
    68221 ggcgataacg ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg
    68281 gccatcgaag cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact
    68341 atttcgtctg gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa
    68401 acatgaaaag actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca
    68461 tggaaccagt cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat
    68521 caaaccaaat gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata
    68581 tcggaaaacc gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta
    68641 gcggatttgg ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag
    68701 cgaacatcca agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata
    68761 tcgcgccgtt ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata
    68821 ctaatttcac ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata
    68881 tgaattatcg tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc
    68941 aaacaacaaa cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat
    69001 tgccatatca agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt
    69061 gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa
    69121 gagaagacga acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc
    69181 tactacatga ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt
    69241 ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat
    69301 tggagaagtt aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg
    69361 cgattgacgc tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt
    69421 tcatggaccc gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct
    69481 tattgcaaga agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt
    69541 caactggagg atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct
    69601 tcttatcttg aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa
    69661 gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct
    69721 gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag
    69781 catgttgcag ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc
    69841 caagtacttg ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt
    69901 gaaacgaaaa aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt
    69961 tgaaataaca aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa
    70021 tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta
    70081 cttaaatgaa gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg
    70141 aaacgtagaa cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa
    70201 caaaacagta ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt
    70261 agctgaacat ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa
    70321 cgatcgattt ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc
    70381 taaggagtga gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt
    70441 tagcaggggc gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga
    70501 ttggaccata ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag
    70561 ctatcgaagc agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag
    70621 cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa
    70681 taaaacttga tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact
    70741 acttgtttct tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg
    70801 aagaactggg acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg
    70861 aattgccaga aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg
    70921 ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt
    70981 tgatcctttt acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga
    71041 ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag
    71101 aatgacattg atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga
    71161 aaatcaattg acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat
    71221 acgaagtatc aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata
    71281 tgatgaattg gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag
    71341 tattttaaaa gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat
    71401 caacgaagct ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag
    71461 tactgattat catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt
    71521 atgtagaatc accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg
    71581 aagataaagg ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt
    71641 tcttagcttt aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga
    71701 ctggtggggc gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc
    71761 aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca
    71821 aaggcgtata tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg
    71881 gagatacagt aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt
    71941 atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag
    72001 aaaaaacagc ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta
    72061 tcacgcttac tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct
    72121 ctatcggtgg gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg
    72181 gtaaaaactt gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag
    72241 aagttggccg aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag
    72301 agattttctt tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa
    72361 taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa
    72421 tcgtaccagt aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact
    72481 aaataattaa agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa
    72541 tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg
    72601 attttgccag agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta
    72661 ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc
    72721 acccaaattg atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt
    72781 tttattatca aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact
    72841 ttcttcgttt agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga
    72901 tgctcgtcca aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta
    72961 ttccttttct tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga
    73021 agaagcggat agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata
    73081 tatccctgct gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg
    73141 aaaacagcct acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc
    73201 agaacttcag cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag
    73261 agaagaaaac ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga
    73321 aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac
    73381 tggcacgagc aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg
    73441 gcaggacgag gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat
    73501 taaaaaaacc aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga
    73561 atacggctgt cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac
    73621 ttagctatac agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata
    73681 aacaactttc taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa
    73741 agactatagt tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca
    73801 aaaatatcgg aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa
    73861 acgccatgat tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag
    73921 aatttttata atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt
    73981 ttgtcagaca ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt
    74041 ttaatcaact tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg
    74101 cgatacgaat aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca
    74161 gtactttatg gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt
    74221 aggagcgaca ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag
    74281 tggagatgtc cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac
    74341 gatccatgca gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg
    74401 ttcagaaatc tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc
    74461 tggtgtgaaa gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt
    74521 gatcgaacaa ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa
    74581 acttcaaaat gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc
    74641 ggttgatttt tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt
    74701 ggggttatcg tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt
    74761 ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag
    74821 agtattcatt tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga
    74881 ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc
    74941 tccagctttc ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag
    75001 gaaagggaat agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc
    75061 tttataattt tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa
    75121 ttcaaaagat tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa
    75181 gaggatcttt tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta
    75241 attctttatt atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg
    75301 agagtggttt gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt
    75361 ggctgtatta tttatttcga cgtatcggat ggccactaca cgtcagtatt attccgttga
    75421 gtctactaac tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta
    75481 gtttgctgtt tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg
    75541 acttatttgc agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg
    75601 cgggtggaca gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag
    75661 attggttgaa aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg
    75721 tatggcattt ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg
    75781 tattcaagtt tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc
    75841 cggaaacttt acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt
    75901 tttcttcagg gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata
    75961 ttttcggatt actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt
    76021 atgttggtct tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga
    76081 tcgcttttat cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag
    76141 aaacgatttt gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag
    76201 gtatgatacg tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa
    76261 gtgtaatcga taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca
    76321 cgttatctca gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg
    76381 agccattaca aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca
    76441 aacatgggga caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg
    76501 gagagggaag aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt
    76561 tatttttagg agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc
    76621 tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt
    76681 ttctttccca tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg
    76741 gtcacccgca cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa
    76801 cggatcaaca aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag
    76861 tggaaacaat gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg
    76921 gtgagtaatt tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct
    76981 tgttacttga ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc
    77041 aaaaaaatcc aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat
    77101 gcaagtttag atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg
    77161 ttagtattcg tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa
    77221 caaaatactg atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta
    77281 ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa
    77341 acagcaatta tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg
    77401 aataccttag agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg
    77461 ttgaccgact tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga
    77521 caaaatcaac agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa
    77581 cataatattt ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt
    77641 ttatacgaag atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt
    77701 tctcaattac gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca
    77761 aatattgctg aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta
    77821 aaaaaattcg atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat
    77881 caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca
    77941 gggcagttgt taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt
    78001 ttgtttttgt cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag
    78061 cgattttttt gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt
    78121 ttgattcagc catatcaata gctttaacag cttctttata taacgcatct acgttgtcag
    78181 cacctgaagc tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag
    78241 atgaattttt cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt
    78301 ttggcattag tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac
    78361 attcattatt atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc
    78421 ttaacagtct tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa
    78481 tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct
    78541 atttaaacaa agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc
    78601 gtttgcaaag ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta
    78661 agaaactgca gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac
    78721 cgattatttt aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt
    78781 tccgacggtt ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga
    78841 tatattttgc caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca
    78901 agttgatccc agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac
    78961 cttcaaaagt ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta
    79021 attcttccat tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta
    79081 atctagtata acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata
    79141 ggaagtctca ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca
    79201 tcgcaggagc atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat
    79261 tttgtatcaa atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa
    79321 gaaaccagcc gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt
    79381 gtcagtatat cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat
    79441 ttgttctatt ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg
    79501 accggtccgg cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt
    79561 gcaacagtga tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc
    79621 actagcccac cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga
    79681 tcacgtagac cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc
    79741 accccaatat tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg
    79801 agcttcataa tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt
    79861 gctacaagta aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt
    79921 cttcgaaaca atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag
    79981 acctattcat aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt
    80041 atttgacaga agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat
    80101 cagaaagatc aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc
    80161 ttccttcgtg tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa
    80221 tcaacaagag aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt
    80281 ttaaagatag ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt
    80341 ctgcgatatc catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg
    80401 ctaaagcagg cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat
    80461 tctctttttt gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa
    80521 tccctacttg ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg
    80581 tatggatgtt ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat
    80641 ctagcagacc taaaacagca attaactgct gatctttaga aagataaatc acggttttgc
    80701 cttgattttc caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag
    80761 gatcatatgc gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa
    80821 aaccagtaat ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat
    80881 attgcaaaat cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt
    80941 gttgtgccaa atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag
    81001 tgagcgttcc tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga
    81061 taccgccttt gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg
    81121 gtgtagcaga tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat
    81181 aaaagctttc atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa
    81241 cagcaagcaa aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt
    81301 gagttggcgt attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg
    81361 aactcgtttt tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca
    81421 tctcatcgcc taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga
    81481 tcgttgcctg tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa
    81541 ataccaagtt atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt
    81601 ttaataattg ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt
    81661 tattagttgt gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc
    81721 cttcaaagta attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca
    81781 cattcagtgt ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac
    81841 cgatgataat ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga
    81901 taaaaccgaa gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat
    81961 tagtcatcgt tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga
    82021 taataataac agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt
    82081 gtttttatta tttataatga ttattattaa agaaaataaa aaaccagaac atttc
//
LOCUS       DANJJW010000003        79081 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000003 DANJJW010000000
VERSION     DANJJW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            219..1193
                     /locus_tag="PWS82_000167"
     CDS             219..1193
                     /locus_tag="PWS82_000167"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0905327.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1363..2763
                     /locus_tag="PWS82_000168"
     CDS             1363..2763
                     /locus_tag="PWS82_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL0905328.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2841..3563)
                     /locus_tag="PWS82_000169"
     CDS             complement(2841..3563)
                     /locus_tag="PWS82_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0905329.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3642..4148)
                     /locus_tag="PWS82_000170"
     CDS             complement(3642..4148)
                     /locus_tag="PWS82_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0905330.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4271..6070
                     /locus_tag="PWS82_000171"
     CDS             4271..6070
                     /locus_tag="PWS82_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL0905331.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6138..7712)
                     /locus_tag="PWS82_000172"
     CDS             complement(6138..7712)
                     /locus_tag="PWS82_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0905332.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(7967..8476)
                     /gene="mreD"
                     /locus_tag="PWS82_000173"
     CDS             complement(7967..8476)
                     /gene="mreD"
                     /locus_tag="PWS82_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL0905333.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8480..9334)
                     /gene="mreC"
                     /locus_tag="PWS82_000174"
     CDS             complement(8480..9334)
                     /gene="mreC"
                     /locus_tag="PWS82_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL0905334.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(9489..10127)
                     /locus_tag="PWS82_000175"
     CDS             complement(9489..10127)
                     /locus_tag="PWS82_000175"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL0905335.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(10128..10778)
                     /locus_tag="PWS82_000176"
     CDS             complement(10128..10778)
                     /locus_tag="PWS82_000176"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL0905336.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(10791..11690)
                     /gene="rsgA"
                     /locus_tag="PWS82_000177"
     CDS             complement(10791..11690)
                     /gene="rsgA"
                     /locus_tag="PWS82_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL0905337.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(11855..13924)
                     /gene="pknB"
                     /locus_tag="PWS82_000178"
     CDS             complement(11855..13924)
                     /gene="pknB"
                     /locus_tag="PWS82_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL0905338.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(13921..14661)
                     /locus_tag="PWS82_000179"
     CDS             complement(13921..14661)
                     /locus_tag="PWS82_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL0905339.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(14674..16032)
                     /gene="rsmB"
                     /locus_tag="PWS82_000180"
     CDS             complement(14674..16032)
                     /gene="rsmB"
                     /locus_tag="PWS82_000180"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL0905340.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(16032..16970)
                     /locus_tag="PWS82_000181"
     CDS             complement(16032..16970)
                     /locus_tag="PWS82_000181"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL0905341.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(16963..17454)
                     /gene="def"
                     /locus_tag="PWS82_000182"
     CDS             complement(16963..17454)
                     /gene="def"
                     /locus_tag="PWS82_000182"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0905342.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(17469..19877)
                     /gene="priA"
                     /locus_tag="PWS82_000183"
     CDS             complement(17469..19877)
                     /gene="priA"
                     /locus_tag="PWS82_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL0905343.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(20130..20435)
                     /locus_tag="PWS82_000184"
     CDS             complement(20130..20435)
                     /locus_tag="PWS82_000184"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0905344.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(20440..21054)
                     /gene="gmk"
                     /locus_tag="PWS82_000185"
     CDS             complement(20440..21054)
                     /gene="gmk"
                     /locus_tag="PWS82_000185"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL0905345.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(21541..21825)
                     /locus_tag="PWS82_000186"
     CDS             complement(21541..21825)
                     /locus_tag="PWS82_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905346.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(21843..22733)
                     /locus_tag="PWS82_000187"
     CDS             complement(21843..22733)
                     /locus_tag="PWS82_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL0905347.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(22753..22971)
                     /locus_tag="PWS82_000188"
     CDS             complement(22753..22971)
                     /locus_tag="PWS82_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL0905348.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(22941..24641)
                     /locus_tag="PWS82_000189"
     CDS             complement(22941..24641)
                     /locus_tag="PWS82_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL0905349.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            24908..25681
                     /locus_tag="PWS82_000190"
     CDS             24908..25681
                     /locus_tag="PWS82_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905350.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(25762..26331)
                     /gene="thiT"
                     /locus_tag="PWS82_000191"
     CDS             complement(25762..26331)
                     /gene="thiT"
                     /locus_tag="PWS82_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL0905351.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(26397..26499)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(26505..27476)
                     /locus_tag="PWS82_000192"
     CDS             complement(26505..27476)
                     /locus_tag="PWS82_000192"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0905352.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(27570..28943)
                     /gene="glmU"
                     /locus_tag="PWS82_000193"
     CDS             complement(27570..28943)
                     /gene="glmU"
                     /locus_tag="PWS82_000193"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL0905353.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(29446..30264)
                     /gene="purR"
                     /locus_tag="PWS82_000194"
     CDS             complement(29446..30264)
                     /gene="purR"
                     /locus_tag="PWS82_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL0905354.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(30431..31255)
                     /locus_tag="PWS82_000195"
     CDS             complement(30431..31255)
                     /locus_tag="PWS82_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0905355.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(31228..31908)
                     /locus_tag="PWS82_000196"
     CDS             complement(31228..31908)
                     /locus_tag="PWS82_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0905356.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(31931..32869)
                     /locus_tag="PWS82_000197"
     CDS             complement(31931..32869)
                     /locus_tag="PWS82_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL0905357.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(33114..33977)
                     /locus_tag="PWS82_000198"
     CDS             complement(33114..33977)
                     /locus_tag="PWS82_000198"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL0905358.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(34119..36692)
                     /gene="mprF"
                     /locus_tag="PWS82_000199"
     CDS             complement(34119..36692)
                     /gene="mprF"
                     /locus_tag="PWS82_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0905359.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            36833..37639
                     /gene="proC"
                     /locus_tag="PWS82_000200"
     CDS             36833..37639
                     /gene="proC"
                     /locus_tag="PWS82_000200"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL0905360.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(37718..39274)
                     /gene="rny"
                     /locus_tag="PWS82_000201"
     CDS             complement(37718..39274)
                     /gene="rny"
                     /locus_tag="PWS82_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL0905361.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(39685..40734)
                     /gene="recA"
                     /locus_tag="PWS82_000202"
     CDS             complement(39685..40734)
                     /gene="recA"
                     /locus_tag="PWS82_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL0905362.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(40843..42084)
                     /locus_tag="PWS82_000203"
     CDS             complement(40843..42084)
                     /locus_tag="PWS82_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL0905363.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(42324..42902)
                     /gene="pgsA"
                     /locus_tag="PWS82_000204"
     CDS             complement(42324..42902)
                     /gene="pgsA"
                     /locus_tag="PWS82_000204"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL0905364.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(43111..44034)
                     /locus_tag="PWS82_000205"
     CDS             complement(43111..44034)
                     /locus_tag="PWS82_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905365.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(44773..46059)
                     /locus_tag="PWS82_000206"
     CDS             complement(44773..46059)
                     /locus_tag="PWS82_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0905366.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(46052..47320)
                     /locus_tag="PWS82_000207"
     CDS             complement(46052..47320)
                     /locus_tag="PWS82_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0905367.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            47572..48033
                     /gene="mscL"
                     /locus_tag="PWS82_000208"
     CDS             47572..48033
                     /gene="mscL"
                     /locus_tag="PWS82_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL0905368.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(48093..48539)
                     /locus_tag="PWS82_000209"
     CDS             complement(48093..48539)
                     /locus_tag="PWS82_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905369.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(48632..48877)
                     /locus_tag="PWS82_000210"
     CDS             complement(48632..48877)
                     /locus_tag="PWS82_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL0905370.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(48992..49570)
                     /locus_tag="PWS82_000211"
     CDS             complement(48992..49570)
                     /locus_tag="PWS82_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0905371.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(49647..50177)
                     /locus_tag="PWS82_000212"
     CDS             complement(49647..50177)
                     /locus_tag="PWS82_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL0905372.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(50181..51041)
                     /gene="rlmB"
                     /locus_tag="PWS82_000213"
     CDS             complement(50181..51041)
                     /gene="rlmB"
                     /locus_tag="PWS82_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL0905373.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(51034..51429)
                     /locus_tag="PWS82_000214"
     CDS             complement(51034..51429)
                     /locus_tag="PWS82_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL0905374.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(51426..52838)
                     /locus_tag="PWS82_000215"
     CDS             complement(51426..52838)
                     /locus_tag="PWS82_000215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL0905375.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(52835..53371)
                     /gene="cysE"
                     /locus_tag="PWS82_000216"
     CDS             complement(52835..53371)
                     /gene="cysE"
                     /locus_tag="PWS82_000216"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL0905376.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(53457..53696)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(53693..55153)
                     /locus_tag="PWS82_000217"
     CDS             complement(53693..55153)
                     /locus_tag="PWS82_000217"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL0905377.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(55323..56480)
                     /locus_tag="PWS82_000218"
     CDS             complement(55323..56480)
                     /locus_tag="PWS82_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL0905378.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(56514..57884)
                     /gene="radA"
                     /locus_tag="PWS82_000219"
     CDS             complement(56514..57884)
                     /gene="radA"
                     /locus_tag="PWS82_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL0905379.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(57947..58474)
                     /locus_tag="PWS82_000220"
     CDS             complement(57947..58474)
                     /locus_tag="PWS82_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL0905380.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            58704..59510
                     /gene="proB"
                     /locus_tag="PWS82_000221"
     CDS             58704..59510
                     /gene="proB"
                     /locus_tag="PWS82_000221"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL0905381.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            59507..60754
                     /locus_tag="PWS82_000222"
     CDS             59507..60754
                     /locus_tag="PWS82_000222"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL0905382.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            60830..61174
                     /locus_tag="PWS82_000223"
     CDS             60830..61174
                     /locus_tag="PWS82_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905383.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(61291..61617)
                     /locus_tag="PWS82_000224"
     CDS             complement(61291..61617)
                     /locus_tag="PWS82_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905384.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(62232..63434)
                     /locus_tag="PWS82_000225"
     CDS             complement(62232..63434)
                     /locus_tag="PWS82_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0905385.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            63551..64066
                     /locus_tag="PWS82_000226"
     CDS             63551..64066
                     /locus_tag="PWS82_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0905386.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(64265..64711)
                     /locus_tag="PWS82_000227"
     CDS             complement(64265..64711)
                     /locus_tag="PWS82_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0905387.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(64758..66236)
                     /gene="msr(C)"
                     /locus_tag="PWS82_000228"
     CDS             complement(64758..66236)
                     /gene="msr(C)"
                     /locus_tag="PWS82_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0905388.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(66590..67219)
                     /locus_tag="PWS82_000229"
     CDS             complement(66590..67219)
                     /locus_tag="PWS82_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0905389.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(67203..67667)
                     /locus_tag="PWS82_000230"
     CDS             complement(67203..67667)
                     /locus_tag="PWS82_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0905390.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(68154..69422)
                     /locus_tag="PWS82_000231"
     CDS             complement(68154..69422)
                     /locus_tag="PWS82_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0905391.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(69653..70390)
                     /locus_tag="PWS82_000232"
     CDS             complement(69653..70390)
                     /locus_tag="PWS82_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0905392.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(70457..71026)
                     /locus_tag="PWS82_000233"
     CDS             complement(70457..71026)
                     /locus_tag="PWS82_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0905393.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            71154..72026
                     /locus_tag="PWS82_000234"
     CDS             71154..72026
                     /locus_tag="PWS82_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0905394.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            72368..73240
                     /locus_tag="PWS82_000235"
     CDS             72368..73240
                     /locus_tag="PWS82_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0905395.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            73237..75195
                     /locus_tag="PWS82_000236"
     CDS             73237..75195
                     /locus_tag="PWS82_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0905396.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(75527..76378)
                     /locus_tag="PWS82_000237"
     CDS             complement(75527..76378)
                     /locus_tag="PWS82_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0905397.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            76501..76968
                     /locus_tag="PWS82_000238"
     CDS             76501..76968
                     /locus_tag="PWS82_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL0905398.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(77231..77713)
                     /locus_tag="PWS82_000239"
     CDS             complement(77231..77713)
                     /locus_tag="PWS82_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL0905399.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(77813..78676)
                     /locus_tag="PWS82_000240"
     CDS             complement(77813..78676)
                     /locus_tag="PWS82_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0905400.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        22412 a        16866 c        14242 g        25561 t
ORIGIN      
        1 agagaatcgt taataaatga taaagaaatt aaaaatttct tcaaagttta atgaaataat
       61 tgatatataa aaatagtgaa ataacgcaca tgtaagcttt tacatataat acaaccttgt
      121 tatctgttat attttatagc caaatagcgt gacatggggc ttgagaaatg ttagcataag
      181 catgggaaaa aacaaatcaa tggaaggatt gatactaaat gactgcagct gcagaaaaaa
      241 aagatcatca aaaagttatt cttgttggag acggcgccgt aggttctagc tatgcctttg
      301 ctctagttac tcaaaatatc gctcaagaag taggtattgt ggatatcaat actgctaaaa
      361 ctgaaggaga cgcaattgac ttgtctcacg ctttggcatt tacttcacct aaaaaaatct
      421 attctgctac ttacgcagac gcacatgatg ctgacttagt tgttattaca gctggcgcac
      481 cacaaaaacc aggtgaaact cgtttagact tagtaaacaa aaacctaaaa atcaaccgcg
      541 atgttgtcac acaaatcgtt gaatcaggat ttaacggtat ctttttagtt gccgctaacc
      601 cagtagatat tttgacttac tcaacttgga aattctcagg tttccctaaa gaacgcgtaa
      661 tcggttcagg tacctctctt gactcagctc gtttccgtca agcacttgcg gaattagttg
      721 acgttgatgc acgtaacgtc catgcttaca tcttaggtga acacggagat tcagaattcc
      781 cagtttggtc acatgcaaat gttgccggct tgcaaatcta tgaatgggta aaaaacaacc
      841 cagaaatcga tgaagaagct atggttaacc tattctttgg cgtacgtgac gctgcttaca
      901 caattatcga gaaaaaaggc gctactttct atggtatcgc agtagcatta gcacgtatca
      961 ctcgtgccat cttagatgat gaaaatgctg ttcttccttt atcagtattc atgaatggcg
     1021 aatatggatt gaacgacatc tacattggtg cacctgctgt tatcaaccgt caaggtatcc
     1081 aaaaagtaat cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct gcttctgcta
     1141 agcaattaaa agaaattcta gatgaagctt ttgctaaact agacgctgaa taatctttga
     1201 acatgacgga aaacatctat tcggtgtttt ccgttttttt attttatcac ccttatggtt
     1261 tctaaatttt ttcataaagt agagataacc ttcgtctttt cctctaaatt atgctatcat
     1321 gagaccgttc caccctaaac ttttacttaa ggagttgtcg atatgacaag atcttcgcat
     1381 cgaaaaaaat cgaaacatgt tgctttgctt tcagttcttg gtgttctctt agtgctgatc
     1441 agcttttata cctatcgcag tacatactac acaaagcatt ttttgccgaa tacagaaata
     1501 aatggaataa atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt aaaagaagct
     1561 tattctgata aaaaactggc tattaaagaa gatggaaaag tctggcagga agtaccaaaa
     1621 tccgagctcg gctacaaaga tgatttcact tcggagcttt ctccgatcct aaatgaacaa
     1681 aacggatgga cttggggaat gacttatgtc tcagcggcag aaaaacaaga aatagatcct
     1741 gtcagtcaaa accaccaaaa actagacact accgttcaga cattgacaac taagctgact
     1801 gatttgaaca aagaccggac acctactact gatgcaacga tcgaaaaagc aggtgactct
     1861 ttcgttatca agcctgaagt caatggaaat acgatcgacg tcgatgctgc ggtcaaacaa
     1921 ttaaaaagtg cggtaaactc tggtaaagat acaattgaat tgactgaatt caaagaaaaa
     1981 ccaaaagtaa catctgaaga cagctcactg aaagaacaac tcgcatccat gaatgctatc
     2041 gcaaatgtaa aagctactta cagtataaac ggtgagactt tccagatccc atcaagtgat
     2101 attatgagtt ggctgaccta caatgatgga aaagtcgatt tagatacaga acaagtacgt
     2161 caatatgtaa cagatttagg cacaaagtat aacacaagta cgaatgacac aaaatttaaa
     2221 agtacaaaaa gaggagaagt tacggtccca gttggcactt acagttggac gatccaaaca
     2281 gacagtgaga cagaagcatt gaaaaaagcg attctggcag gccaagattt tactcgttca
     2341 cccattgtgc aaggtggcac aaccgctgat catccattga tcgaagatac gtatatcgaa
     2401 gttgatctgg agaaccagca tatgtggtat tacaaagacg gaaaagtggc ccttgaaaca
     2461 gatatcgttt ccggaaagcc aactacacct acacctgctg gagtcttcta tgtttggaat
     2521 aaagaagaag atgcaacttt aaaaggaaca aatgacgatg gaacacctta cgagagtccg
     2581 gtaaactact ggatgccgat tgactggaca ggtgttggta tccacgattc agactggcaa
     2641 cctgaatacg gtggagactt atggaaaacc cgcggttcac acggctgcat caacacccca
     2701 ccaagtgtaa tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt attggtcttc
     2761 taacaaaaat aatatataaa aagaaagcac ccgtacgatt ggtacaggtg ctttcttttt
     2821 attatctaac aatgtgctac ttacatttca gcagcagtaa agacttctga aacatcatca
     2881 tcgtcttcta gcttatctac tagacgttcc aattgctctt tttgttcctc atttaaatca
     2941 atcgtcgttt gtggaatcat cgtcaattct gcttgcgcca aagtgaactc tttttccaac
     3001 tcatctcgta cagcagcaaa atcttctggt gcagtataaa tctcgaatac ttcaggagaa
     3061 gtttgcagat cttctgcccc cgcctctaat acatcctcaa acatcgtctc ttcatcaaca
     3121 gtcaacccat cgcgttcaat agcgatatat cctttgcggt caaacaagta actgactgaa
     3181 cccgtttctc ccagtgatcc accgtttcga gtaaaagcta ctcggacatt tgtcgctgtc
     3241 cgattacggt tatctgtcaa tgcatggact aagattccta ctccacccgg accatatcct
     3301 tcatacgtga tttcatcgta attcgtttca tctacggcac tcgtagcctt tttaatcgcc
     3361 cgttccacat tgtcgttagg catatttgca gcttttgctt tgtccacaac aagccgcaaa
     3421 gctggattca tagatggatc aggaccgcct gctttagctg ccatataaat ttcacgagaa
     3481 agtttctgaa agactttccc tcgttttgca tcttgggcat tttttcttcc ttggatatta
     3541 tgccatttac tatgtcctga catgattttt cctcccttgt tcttttgaga atacacttct
     3601 attctagtac tatttcacaa aaaaacaaag ccacagccaa tctataaacg cttcttgaat
     3661 gccgcaaaaa gcacacataa tatcacagca gtcagagcac cgatcgaatc caacatgaca
     3721 tcttggaaca taggtgttcg tccaccggtc atcatctgat ggaattcgtc caaacctgcg
     3781 taccctgttg ctgaaagcca agcaaggatc gctgtcagcc accaaatttt tagcttgggg
     3841 aaaacaccta aaaacaagct gcctcccaaa ataaaatagg taccgaaatg tgcgcccttc
     3901 cttaaaaaga actctaaaaa tttggcgtag cccagatggt tgatactcac catacttcca
     3961 ccataattaa aacgtatacc cgctaatgtg tctttaaatg gctgattagg cagccagctt
     4021 tctaatcgcg aaacttgtga ttgctgtccg taagtttgag aagaactgta aaataagatt
     4081 ataacgacta tcagtgcgat tactaaataa atatttccgt ttttcatttg tttttttgaa
     4141 gaattcataa gtcacctctg tattttatct tagacttttt ttgaagaaac aacaatcatt
     4201 ttcatagaga cttcttccat aaaaaagtgc tatacttaat tttgtattac aatatgaagg
     4261 gagttttttc atgagttatt cattaaattg gaatctagat tctatttttt ccggaggcag
     4321 tcattctgat gctttgaacc aacggatgaa gcaattggaa gaccaaatga acgagtatta
     4381 tcaccgcgta acaaaatggt cacctagttc tgacaatgca gagcagttaa acgctattct
     4441 tcaattgcaa gaaacgatta cgaatggttt tacccaatgc agcagctata ttactgcatt
     4501 gctttctgcc aatgtcaatg attcagatgc aaaggtctta tcaggcaagc tttacgcaat
     4561 gctgcctcgt ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag aaatatctga
     4621 caatgattgg aatcagatgc tttctcaatc ttcgtttgaa acaattgctt ttcgtttaaa
     4681 cgagattcgt cgtgatggct cacaattatt atcagaagct gaagaaaaca ttataaatac
     4741 gctttcttta gatggtctaa atgcttggag cagtcattat gatacgattg tagccagcat
     4801 ttcgattccc ttcgaacaag atggacaagt agtcgaatta tctgctggac aagccttcaa
     4861 taagatgatg ggcgatccag atccaaaagt cagagaaaca cttttcgcag catgggaaga
     4921 agcctggaaa gaaaaggcac ccctcttcac ggatactttg aatcatttag atggttttag
     4981 actttctgac tatgaacttc atggtgtcac agattttcta caaaaaccat tagagtacaa
     5041 tcggttgaag aaagaaacat tgtctgtcat gtgggataca atccagaaaa acaaacaacc
     5101 ccttgttaat tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa tggaatggca
     5161 agatcaagat gcacctatta ttttaggcga tttgaaagaa aaaaccttta gttttgacga
     5221 agcagcagca tttattatcg aaaactttaa caaattcagt ccaaaaatgg ctaaatttgc
     5281 ccaatctgct tttgaaaaga gctggatcga agcagaagat cgtcctggga aacgtcctgg
     5341 tggttattgt actgagttac cagaaacaaa agaatcaagg atctttatga cctacagtaa
     5401 ttctgttaat gaggttgcga cacttgctca tgaactagga catgctttcc atagcagcac
     5461 gatgtgggat ttaccttctt taaatcgcga atatgccatg aatgtcgcag aaacagcaag
     5521 tacattcgca gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa caaaagaaga
     5581 aaaaatcaat ttgctagata caaaactgca aaatgcttta gctatgttca tgaatatcca
     5641 ttcccgattc atttttgaaa atcgattcta tgaagcacgg caagaaggat tagtctcaga
     5701 agataagatc acagagatga tggttgaggc gcaaaaagaa ggctatcaag gagctttagc
     5761 tacttaccat ccatatttct gggctgcaaa attgcatttc tttattgatg acgtaccgtt
     5821 ttataacttc ccatacacat ttggctacct ctttagtttg ggcatctatg cttacgccaa
     5881 ccaaaaggga gcaaattttg aagaagaata tatccattta ctacgcgata ctgcctcaat
     5941 gacgacggaa gatcttgcga agaaacattt gaatgtcgat cttacccaac cagatttttg
     6001 gcaagcaggt atcgacatgg tgactgaaga tatccaaacg ttcatggaat tgacagaaga
     6061 atttatctga tttctatttt atcacaataa aaaaagagtt agtccagaat gggctaactc
     6121 tttttgatta atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa
     6181 ccaactttta cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca
     6241 atcgctacgt ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct
     6301 tgagaaactg aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca
     6361 cgaccagtta cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga
     6421 tctttaccgc cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct
     6481 gcaccattca cacttccaga aggagctgga gcaggtgtta caggttttgg ttgttcaggt
     6541 gttggtgttg gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt
     6601 tgatctgttg atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca
     6661 ggcactgttg ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt
     6721 gctgaagatt caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat
     6781 tcttctgttg aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat
     6841 gatgattctt cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt
     6901 gcagtagatg aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt
     6961 tcagcttttt cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga
     7021 atacgagctt gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct
     7081 tcagcagttg cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta
     7141 gataaaagat cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt
     7201 ttagcatcgt tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc
     7261 atcaagtcgt tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct
     7321 gctaatgaat cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt
     7381 gcttcgcgtg cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct
     7441 gcaatgtctt ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg
     7501 tcttgtgctt gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt
     7561 gcatctgctt gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa
     7621 tcaaaatcgt ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat
     7681 accattactg ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta
     7741 ataaatatct aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg
     7801 tgtcaacgat ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa
     7861 aactgttcgt ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga
     7921 taacaacgat tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga
     7981 ataatttctt aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag
     8041 gtcctaaaaa ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact
     8101 ggaccaccat cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag
     8161 taaagatgtt gacatggata acgtctttca ttacatacat caaccataca attaaaggga
     8221 gagctactgc atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa
     8281 caatcgtggt aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca
     8341 atatcagaaa gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg
     8401 ttaaatgacc atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg
     8461 tttctttctt caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca
     8521 cgtcatacat ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg
     8581 gtttcgtttt aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg
     8641 atgtctggac aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta
     8701 aggcatggag cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag
     8761 atacccgtac tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag
     8821 acgaagccgt attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca
     8881 tatttgcttc gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt
     8941 caggcgaacg agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca
     9001 actcaagttc ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa
     9061 cgtcatcata gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa
     9121 gattatggac agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat
     9181 caaccattgc aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg
     9241 ctcgctgcgc agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa
     9301 taataatatt tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc
     9361 ttgtgttcaa ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct
     9421 tttttttagc aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg
     9481 tttatggatc aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct
     9541 gcggtatctg aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt
     9601 tccaatacat acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa
     9661 gctaaatact tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac
     9721 aaataatttt gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc
     9781 atacctaacc agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa
     9841 gtgacttccg cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta
     9901 tcgtcttttt ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc
     9961 tcttcgttcg acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga
    10021 agcaggtaca gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac
    10081 tgcggccagt tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc
    10141 tcttttaatc gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca
    10201 acgaagacat ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca
    10261 tctacttcga ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt
    10321 ttttctactg tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact
    10381 agtacttgat cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg
    10441 ataactactc cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga
    10501 ggagttgctt cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg
    10561 tagttttctg gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg
    10621 attgcctgaa caatatttgg ccctaatgtg atatttggga caaatcggcc atccattaca
    10681 tctacgtgga tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg
    10741 gcaaaatctg cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt
    10801 ttaccttctt tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa
    10861 taattgtcgt aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag
    10921 ccaggttctt tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat
    10981 tcaggaaatt gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat
    11041 cctggtgtat ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta
    11101 tgccgtcctc ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat
    11161 atttgattca gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt
    11221 tctgggaagt aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga
    11281 tagcctattt tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag
    11341 tcggtctttg tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag
    11401 cggtcaagta gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga
    11461 tcaacatttg tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga
    11521 tagccgtctg tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt
    11581 ttccgattcc ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca
    11641 tagtaaaaac cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt
    11701 gtttatttta ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat
    11761 atggcttgaa aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat
    11821 gtcgcattcc cttctgatat attttattca gcacttattt tgaactttct gaggtgctag
    11881 ctgatgaact tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc
    11941 tacttgagct gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg
    12001 gtcctttaga gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg
    12061 gtactgtacg aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc
    12121 cgatatattc tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg
    12181 agatatcatt caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt
    12241 ctgctacaac ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt
    12301 cgctatagtc ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat
    12361 tacgaacagt ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt
    12421 tgctgactgt gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag
    12481 attgcgaaat gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca
    12541 agtcagcaga aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac
    12601 tgtaatcatc taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt
    12661 tttgcttgac tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt
    12721 tgtcatctgc aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt
    12781 ctctagcaga agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc
    12841 cagaagcaaa gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca
    12901 gccaccattt tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta
    12961 aggtttcgtt ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg
    13021 gtgcatcttc cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc
    13081 atttttcttc gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct
    13141 cggctgactt ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca
    13201 gtgcttgtgg cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt
    13261 ttaaagcaat tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat
    13321 aaaggatgat ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct
    13381 gctcagggga cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt
    13441 cagataaagc gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca
    13501 gtacgttttg gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag
    13561 cagaaaggat ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt
    13621 ggatatatcg tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt
    13681 cttcttcacc aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta
    13741 aagcttctct ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga
    13801 ctttaactgc tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac
    13861 caccgctacc gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga
    13921 tcatacactg tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc
    13981 ttcattggca cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat
    14041 gatcgttcct atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag
    14101 tcgatctttt agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc
    14161 gacagaacgt gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc
    14221 tcctgatttg actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc
    14281 ttcacgaatc aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga
    14341 cagagcggcc gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg
    14401 ctgataaatc agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc
    14461 atcgtcaaca gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact
    14521 ggcaatgtct ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc
    14581 tgaacggctg gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc
    14641 tgattgatat tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa
    14701 aaatccatct gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga
    14761 ttgtagcata ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa
    14821 agcagaaaca acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat
    14881 accggaagac tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa
    14941 attcgctaat tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc
    15001 aagacctgaa caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt
    15061 actgacttct ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc
    15121 tgcattatct tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc
    15181 accgcatgac ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca
    15241 ggcatctaag acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact
    15301 ttcatcttgg atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt
    15361 tttcgctact ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg
    15421 caaaacagtg atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg
    15481 gctaggctga tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac
    15541 cagtcgtttc gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc
    15601 agcaatgcct tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac
    15661 aaatttccct gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat
    15721 agcatgtgca gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat
    15781 cttgacccag tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa
    15841 tagtcgctga tggctaatcg taccatagat caattctgta aataaaccca catctttttg
    15901 atttagctcg ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt
    15961 gttcactcgc tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg
    16021 gatttttttc attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg
    16081 aaatcatgta cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg
    16141 gtctgtttac cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt
    16201 gctttgtctg tagtttctgc caatacttca accgataata atttccagcg tatgccctca
    16261 taggtcgtga atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct
    16321 gcagttttat gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct
    16381 tctgactcgt cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc
    16441 aatagctttc ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta
    16501 gtgattggta tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc
    16561 atgatcgtta caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct
    16621 ccccgatatt taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc
    16681 aataactttt caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat
    16741 tcaatgattt cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata
    16801 ccgtgcttca gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact
    16861 ggacggtcgg gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca
    16921 agaatcggca cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc
    16981 ctccatatat tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa
    17041 gataccgtct aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt
    17101 cacttctatc tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt
    17161 gaccgttcca tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt
    17221 tgcttcgatg atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac
    17281 agcaattctc ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat
    17341 cattgtctca tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg
    17401 ctgagcagtt ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg
    17461 gccctttctt aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc
    17521 tgttctttct ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt
    17581 ttatatttga tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc
    17641 gttggcccta atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga
    17701 aagattttct ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag
    17761 taataaggag gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca
    17821 taattttgtt ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg
    17881 acttcgcctg ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat
    17941 gtatgctcac tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca
    18001 actaatgtca cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata
    18061 tctgcttctt ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg
    18121 gtatctacat ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct
    18181 actttttgag tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga
    18241 ttgggaatag gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc
    18301 agtgtaagag agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc
    18361 acaaaagacg aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat
    18421 cgattttgta ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac
    18481 tcttcacgca tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg
    18541 tttaactgca ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc
    18601 gtcgcgcttc ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct
    18661 ctcgcatgat aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata
    18721 atgatcagcc cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact
    18781 tgcgcttctt cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg
    18841 ctatgtaaga ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga
    18901 gtcaatgcga tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg
    18961 attgcctgaa gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc
    19021 tgatcttttt gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca
    19081 ttcagttcca atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt
    19141 tctgattcta taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg
    19201 attcctttat ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa
    19261 agaagttctt tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc
    19321 ttttcagtta acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact
    19381 tcatatttga tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct
    19441 tcttcttctg cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct
    19501 ggtgtgtctg ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc
    19561 atcgtttgta gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat
    19621 gaaagtaatt cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa
    19681 gatggattag ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga
    19741 ttgccattac caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca
    19801 gagggaacca aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact
    19861 tcagctgtta atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa
    19921 aaagttcctt gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct
    19981 aacaaaaaat taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc
    20041 cttttctacc agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc
    20101 tttttagatg ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat
    20161 tccaattcgt gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa
    20221 cgttcacgtt ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct
    20281 aaagcttgac cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca
    20341 tccaattcat gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga
    20401 tctaatagtg agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt
    20461 aatatattta ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat
    20521 tcgttcaact gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat
    20581 ttcgatttct tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc
    20641 tcggcctacg atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac
    20701 accatcgggc actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac
    20761 atcttttcct tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc
    20821 gacatattct gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt
    20881 gcttcggaag aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat
    20941 tgagatcgaa tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc
    21001 ttttcctaca ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac
    21061 gtcctttcaa ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt
    21121 caaggttatc tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc
    21181 gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt
    21241 ttttcgtttg gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg
    21301 tcgtgaagaa gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac
    21361 atttttgcat aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa
    21421 ataaaaaaag attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag
    21481 aagctaagcg aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg
    21541 ctaaaagccc atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg
    21601 tgctttttct gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt
    21661 catcgtcacg taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc
    21721 acaggcatct ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac
    21781 agtttttcca cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt
    21841 cgtcattcaa tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg
    21901 acttgatttt taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc
    21961 atttcttgga tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca
    22021 tctagttttt gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc
    22081 aacaagatgg ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc
    22141 tccagctttt tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg
    22201 agttttttta gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct
    22261 tgaccttctt tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga
    22321 gctaactgtg ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt
    22381 tcgtccagca cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat
    22441 cgttgttgaa ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg
    22501 acgatctgtt tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg
    22561 cggcttaact gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta
    22621 ggacttcgta tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac
    22681 tgatacgctt ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag
    22741 ggtccttcct cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt
    22801 caatgtcagg cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt
    22861 tggcgggcga ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc
    22921 tgacaatata gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc
    22981 tccttgagat aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca
    23041 tgctgcaaac gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg
    23101 cgctcatttt gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat
    23161 tgaccaattt gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt
    23221 tgaatcatca tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa
    23281 agttctagca tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc
    23341 agtttttgcc aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt
    23401 aagtaagaca aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac
    23461 cagttcccat agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt
    23521 aattggttcc tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat
    23581 ttccaatgga taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca
    23641 tctacgacaa atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg
    23701 cgatacccac cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa
    23761 aatgaagcag aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca
    23821 gtcactactt ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga
    23881 ttatgtttga gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa
    23941 gaaaccccgc cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt
    24001 tcacgaaggt cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc
    24061 tttttccata aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac
    24121 gcacctaccg aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc
    24181 cacacaccaa tctgataagc accgtgcgct cctcctccac ctaaaacaag tgctgtatga
    24241 tagaccagct ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc
    24301 aaccattctt ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa
    24361 cgagcgcaag cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc
    24421 aacagattat taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat
    24481 tttttcgata ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta
    24541 ctttcttgat gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac
    24601 agcgacattt tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc
    24661 aagtatacca gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat
    24721 aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat
    24781 tgaaaatgag agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga
    24841 aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag
    24901 gaacatgatg actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac
    24961 aaaagggctg actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct
    25021 cttgcttgat ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat
    25081 ccgtggtaca gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gtggattcga
    25141 tgaattgaag tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga
    25201 aatttcagaa ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta
    25261 taaactacag aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta
    25321 tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga
    25381 taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt
    25441 ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca
    25501 agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg
    25561 gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt
    25621 caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta
    25681 gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca
    25741 agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg
    25801 agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct
    25861 ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc
    25921 ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc
    25981 accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac
    26041 atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa
    26101 cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat
    26161 gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat
    26221 ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat
    26281 cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact
    26341 tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc
    26401 tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag
    26461 attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt
    26521 tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt
    26581 ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag
    26641 tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat
    26701 aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg
    26761 gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg
    26821 cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg
    26881 tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca
    26941 ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa
    27001 ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt
    27061 tggactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt
    27121 gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag
    27181 ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc
    27241 atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag
    27301 acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga
    27361 gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg
    27421 cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg
    27481 acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt
    27541 taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat
    27601 ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct
    27661 gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata
    27721 ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca
    27781 tcatagttga cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca
    27841 ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc
    27901 acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa
    27961 ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg
    28021 acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa
    28081 ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt
    28141 tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata
    28201 tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca
    28261 ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct
    28321 tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca
    28381 actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt
    28441 tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc
    28501 ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag
    28561 atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta
    28621 gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa
    28681 gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta
    28741 ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact
    28801 tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa
    28861 acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa
    28921 ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt
    28981 tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc
    29041 tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg
    29101 ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc
    29161 gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa
    29221 tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt
    29281 tattgagcta atactcagga caggacgctg atttcacaac ctctagataa acggtgttca
    29341 aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca
    29401 aaatttttct tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata
    29461 atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag
    29521 tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat
    29581 gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc
    29641 gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga
    29701 cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga
    29761 tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga
    29821 cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc
    29881 accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa
    29941 caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc
    30001 tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac
    30061 accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt
    30121 cttgatgatt cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa
    30181 gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag
    30241 acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta
    30301 cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc
    30361 ggtatttatt aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt
    30421 tctttataat ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta
    30481 caatcaggaa caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat
    30541 aaaacgagct agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa
    30601 tcacgacatt gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg
    30661 cggatatcaa aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca
    30721 cattgaacat catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat
    30781 caaaagtcag aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga
    30841 ataagattcc taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat
    30901 aggattggat acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg
    30961 ccaaaccacc agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc
    31021 gcagatactc caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat
    31081 taaggttcaa aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag
    31141 ccatcagcga ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa
    31201 tgaagatagc ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga
    31261 atcctccttt ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc
    31321 atgggtgatc attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata
    31381 aaattcattt ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc
    31441 agggtcagta gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc
    31501 acctattcgc cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg
    31561 ttgatgatct ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg
    31621 gactaattcg atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat
    31681 ataaccgata cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt
    31741 tgaaggtttc agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc
    31801 tccagttaaa atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg
    31861 ttcatcatca tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc
    31921 attctaaaac ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa
    31981 tataattttc gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt
    32041 ctagcttcac tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt
    32101 caaaataaat cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac
    32161 ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt
    32221 gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt
    32281 ttagctcatc ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat
    32341 tcttcgtaaa agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc
    32401 tgacttcttt tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat
    32461 gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga
    32521 ttcctttagc tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat
    32581 ccacaaaaaa ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg
    32641 ctctttcttt tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt
    32701 tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg
    32761 taacgatcgt caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa
    32821 acagtaaagc acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc
    32881 tttcaatgaa caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa
    32941 aaaacacagt caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa
    33001 tcgtaaaatg gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag
    33061 aataccattt taggattcct cagtttttca tcacctattt tacttcttat attttattgc
    33121 agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc
    33181 gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca
    33241 gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt
    33301 cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct
    33361 cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct
    33421 ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt
    33481 ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg
    33541 tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac
    33601 gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt
    33661 cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt
    33721 ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct
    33781 accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa
    33841 aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct
    33901 tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat
    33961 tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata
    34021 aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag
    34081 aattttctct tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct
    34141 tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta
    34201 gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca
    34261 tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat
    34321 acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat
    34381 ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg
    34441 gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat
    34501 cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta
    34561 cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag
    34621 tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat
    34681 tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat
    34741 tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca
    34801 cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc
    34861 ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct
    34921 gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg
    34981 atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa
    35041 cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca
    35101 aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa
    35161 agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca
    35221 tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg
    35281 tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt
    35341 atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca
    35401 gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta
    35461 tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact
    35521 gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct
    35581 aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta
    35641 gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg
    35701 atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg
    35761 actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac
    35821 aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt
    35881 cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct
    35941 atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc
    36001 aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt
    36061 agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa
    36121 gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt
    36181 cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca
    36241 gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta
    36301 tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg
    36361 gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt
    36421 tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga
    36481 gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc
    36541 atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc
    36601 acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc
    36661 cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt
    36721 tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt
    36781 gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt
    36841 aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg
    36901 aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt
    36961 tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga
    37021 tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga
    37081 atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga
    37141 aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag
    37201 cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca
    37261 gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt
    37321 cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc
    37381 gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc
    37441 aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact
    37501 tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga
    37561 agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga
    37621 gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc
    37681 tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac
    37741 gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc
    37801 gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga
    37861 ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc
    37921 tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc
    37981 gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag
    38041 aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt
    38101 tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa
    38161 gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa
    38221 gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt
    38281 gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg
    38341 tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt
    38401 ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta
    38461 tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa
    38521 caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc
    38581 gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa
    38641 cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata
    38701 ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca
    38761 ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt
    38821 cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt
    38881 caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac
    38941 tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc
    39001 gttgaatcaa acgattctct tgagatttta attctaactt actttctctc agctcacttt
    39061 caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta
    39121 gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag
    39181 ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta
    39241 aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt
    39301 catttttatc atctatacag cttctatgat aaattattct aaactcgtca atatgcatct
    39361 tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg
    39421 tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa
    39481 ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt
    39541 attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact
    39601 cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc
    39661 agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt
    39721 ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga
    39781 aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg
    39841 accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc
    39901 tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac
    39961 ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt
    40021 accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt
    40081 tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc
    40141 tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc
    40201 agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc
    40261 catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat
    40321 atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc
    40381 aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc
    40441 taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat
    40501 agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg
    40561 tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat
    40621 tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct
    40681 cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat
    40741 aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat atctttttta
    40801 gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg
    40861 ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct
    40921 ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt
    40981 tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta
    41041 atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac
    41101 tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag
    41161 cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc
    41221 ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt
    41281 cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc
    41341 ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac
    41401 atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat
    41461 cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc
    41521 aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc
    41581 tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag
    41641 aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt
    41701 tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt
    41761 tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag
    41821 agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa
    41881 tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa
    41941 acgctgctga ttatctccta caactgtttg ataataaact tcgataccta gatcagctag
    42001 ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc
    42061 gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt
    42121 ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc
    42181 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
    42241 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
    42301 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
    42361 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
    42421 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
    42481 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
    42541 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
    42601 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
    42661 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
    42721 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
    42781 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
    42841 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
    42901 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
    42961 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
    43021 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
    43081 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
    43141 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
    43201 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
    43261 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
    43321 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
    43381 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
    43441 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
    43501 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
    43561 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
    43621 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
    43681 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
    43741 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
    43801 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
    43861 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
    43921 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
    43981 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
    44041 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
    44101 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
    44161 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
    44221 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
    44281 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
    44341 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
    44401 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    44461 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
    44521 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    44581 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
    44641 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
    44701 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
    44761 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
    44821 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
    44881 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
    44941 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
    45001 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
    45061 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
    45121 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
    45181 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
    45241 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
    45301 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
    45361 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
    45421 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
    45481 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
    45541 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
    45601 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
    45661 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
    45721 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
    45781 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
    45841 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
    45901 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
    45961 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
    46021 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
    46081 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
    46141 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
    46201 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
    46261 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
    46321 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
    46381 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
    46441 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
    46501 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
    46561 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
    46621 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
    46681 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
    46741 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
    46801 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
    46861 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
    46921 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
    46981 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
    47041 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
    47101 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat
    47161 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
    47221 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
    47281 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
    47341 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
    47401 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
    47461 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
    47521 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
    47581 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
    47641 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
    47701 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
    47761 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
    47821 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
    47881 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
    47941 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
    48001 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa aagaaagccg
    48061 attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg
    48121 tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac
    48181 tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt
    48241 ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg
    48301 tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg
    48361 taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc tccatcaatg
    48421 tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg
    48481 acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact
    48541 aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac
    48601 gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct
    48661 caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt
    48721 caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt
    48781 ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt
    48841 ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact
    48901 ataccacata tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa
    48961 taaataagaa gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt
    49021 tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta
    49081 aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt
    49141 ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa
    49201 ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt
    49261 tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca
    49321 acagagaata gatttgattg cttataacgc ttttataata acttccaaag ctaacacctt
    49381 tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat
    49441 cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat
    49501 atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact
    49561 cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga
    49621 ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt
    49681 tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa
    49741 tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat
    49801 tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg
    49861 cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata
    49921 taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa
    49981 ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca
    50041 tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc
    50101 tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc
    50161 aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc
    50221 aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc
    50281 aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact
    50341 aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca
    50401 aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt
    50461 gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca
    50521 actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa
    50581 atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct
    50641 tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc
    50701 gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga
    50761 acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct
    50821 cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact
    50881 gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact
    50941 gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat
    51001 ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg
    51061 aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc
    51121 catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct
    51181 cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag
    51241 atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt
    51301 tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac
    51361 atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta
    51421 atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct
    51481 ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct
    51541 tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat
    51601 aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg
    51661 gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact
    51721 gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa
    51781 ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg
    51841 cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc
    51901 atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc
    51961 aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat
    52021 ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta
    52081 gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat
    52141 tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata
    52201 acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc
    52261 tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt
    52321 tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat
    52381 ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca
    52441 tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact
    52501 cttggatgca gtgtagcagg ttcgacattc aacgctttcg tatcttcttc aaatgctcgg
    52561 atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt
    52621 ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa
    52681 tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta
    52741 tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct
    52801 tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc
    52861 ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt
    52921 tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata
    52981 tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt
    53041 cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg
    53101 cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg
    53161 ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca
    53221 ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa
    53281 aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg
    53341 ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta
    53401 tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa
    53461 aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa
    53521 cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt
    53581 gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac
    53641 ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca
    53701 gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca
    53761 attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc
    53821 cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta
    53881 ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac
    53941 cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag
    54001 acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct
    54061 tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat
    54121 aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta
    54181 cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga
    54241 acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca
    54301 ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga
    54361 taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga
    54421 tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt
    54481 gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat
    54541 gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt tggatgacaa
    54601 agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg
    54661 cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt
    54721 tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc
    54781 ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt
    54841 atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag
    54901 attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca
    54961 accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag
    55021 tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata
    55081 atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc
    55141 gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc
    55201 cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt
    55261 ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt
    55321 ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct
    55381 gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca
    55441 acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg
    55501 tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc
    55561 atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga
    55621 acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca
    55681 ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag
    55741 tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct
    55801 aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt
    55861 tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata
    55921 tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt
    55981 tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt
    56041 tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc
    56101 aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga
    56161 atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca
    56221 ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt
    56281 ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg
    56341 cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc
    56401 tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa
    56461 gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg
    56521 aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc
    56581 gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg
    56641 acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt
    56701 tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg
    56761 acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag
    56821 taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga
    56881 gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt
    56941 gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata
    57001 gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact
    57061 tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg
    57121 ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc
    57181 gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga
    57241 ccgacgataa aaatggctat cccatttgtt ttagcggttt tgagtagttc tgcagtggtt
    57301 tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg
    57361 atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc
    57421 atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt
    57481 aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct
    57541 gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca
    57601 atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat
    57661 tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct
    57721 ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg
    57781 accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac
    57841 ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt
    57901 atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag
    57961 aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca
    58021 aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt
    58081 tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa
    58141 tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag
    58201 caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg
    58261 gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca
    58321 accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa
    58381 aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat
    58441 atttagaaat aacttcaaat cctctttttc tcaccggttt tcctccctaa ataaactact
    58501 cctattctaa catatccgag gctgtaaaag actttcttta caaattcttc aagaaattct
    58561 gctatcttaa agtaaaccat taattcgttc tttttccaaa cgactgcaag ttgtacaaaa
    58621 aaactatttt ttacatgact ttactcgtca aaacaagtag aatagaacga aattaatgag
    58681 tggatatttg attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa cgaattattg
    58741 tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa tatcaatctt gcggcaatcg
    58801 atcagttagc ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg atcttagtct
    58861 catcaggagc tgtaggcgtt ggtatgcata aattacattt gtcagaacgt ccgactttga
    58921 ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga actgatgaat atctataatc
    58981 aaagattcag tgtatataat caacagacgg cacagatcct cgtgacaaga gacgtggtag
    59041 attatcctga aagtcgtaaa aatgtgagga atacattgga acagcttttg attatggaca
    59101 cgatccctat cgtgaacgaa aacgatactg tagcagtgga tgaattagat catctgacaa
    59161 aatttggcga taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg gcagaccttt
    59221 tgatcgtcct ttctgatatc gacgggtttt attcagataa tccgactaca aatcccgagg
    59281 ctcagatgta tcgctatatc gataaaatcg atgaaaagat tctggcacaa gcaggtggtg
    59341 ctggtagcac atttggaaca ggcggtatgc agagtaaatt aaaagccgct tctaggatat
    59401 ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa tccggcaatt atttttgata
    59461 ttcttgcagg aaaagaagta ggtactttat ttaaggagga cttgaaatga ctgacttgat
    59521 gcaattagga caacaggcaa aagatagcgc gcaacaacta gcacttatgc ctacaaaccg
    59581 caaaaatgag ctacttgttc aaatggcaca aaccatcaaa aagaatcaac aagcgatcat
    59641 tgaagcaaat cataaagact tagaaaaagc agtcgaaaac aatatcagtg aaacgatgca
    59701 agaccgcttg cagctgacac ctgagcgaat caatgccatg gctacagaga tcgaaaaaat
    59761 tgcttcgtta gacgatccta ttggaaaagt agatgagatg tggacgaaca ccgacggatt
    59821 gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc atcatttacg aatctcgtcc
    59881 aaatgtcacg acagatgcag ctagtctagc attcaaatca gggaatgccg ttatccttcg
    59941 cggtggcaaa gaagcattct tctcgaatca attattagtc caattattgc agcaagtact
    60001 ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc gatgatactt ctcatgaaac
    60061 tgcaggacag ttgatgcgat tgacagaata cttagatgtg ctgattcctc gaggcggagc
    60121 aaaattaatc caacgagtca aagaacaggc aacggtacct atcattgaaa caggtacagg
    60181 aaataatcac gtatatatcg ataaagaagc acagttatct atggcagtga acatcatcgt
    60241 caatgcaaaa gcttcccgtc catctgtctg taacgcggca gaaacattgc tgatccattc
    60301 tgagatcgca ccttttttct tgccagctat tgaaaaagaa ctggtagaac acggggtctc
    60361 tttacgtgcg gatgctcgtg cgttagaata tctagagacg gctgctttag cagaagacac
    60421 agattgggat actgaatatc tagattatat ccttgctgtc aaagtggtcg attctttgga
    60481 tgaagcaatt gcacatatca atcggcataa tactaaacat tcagaaacga tcgtgacaga
    60541 tagttatgcg gctagtcaac gtttcttaaa tgaagtggac gcggcggctg tctatgtcaa
    60601 tgcctccact cgttttacag acggatcggt atttggtttt ggtgcagaga ttggtatctc
    60661 tactcaaaag ttgcatgccc gtggtccaat ggggttaaat gaattaactt ccacaaaata
    60721 tatcatttat ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca ttattcccgc
    60781 cttttgcctt ttttctgtaa aggttctatc caactagaaa ggtgtcaata tgtttgaaat
    60841 tttaatgatt ttttttattt accttatttc tctgaatatc gctgcttttc tgggagtcgg
    60901 tattttatcc cttttctttc aattcaaaaa acgaagcata gggagtcaac gggaaaaatg
    60961 gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc acattagtta
    61021 catggttgca ttatgcttat tagcgacaat caattactat tcttttttcg atcattcaat
    61081 cgcttatacg attacgctac tgattgctgg gatttttcac ttatcttata aatatcagct
    61141 gaataaaaac cacttgaatc ggactttccg ctaaaaaata tccgtacgat aagtagagtg
    61201 ccttgaagat cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag attttgtcac
    61261 agcctctttt tccatataaa cggcgttcgc ctaccagctg cctggtaaat ctgtttcctc
    61321 ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa gcagctcatc
    61381 catggacaag ttcatcgttt tttctttttc ttcttccgct gattcagcca gtagctgttc
    61441 ccgatacacc tgatacatac cagctatttt ttgctgcatc gcttttctag tttcaccttc
    61501 cgcattgatt attggcaatt ctttgatata gccacgataa atcaaccgat catctgaaaa
    61561 ttcaatgggt aattcaacga gtgtcaaccc gacttcctga aaatattctt ccagcatcta
    61621 ttatcccccc attctttttc ttaaatgaat tttagcttac ctactgaaaa ttttccagtt
    61681 tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc ctttttccga
    61741 ataaatggtg ttcttctgcc aactcctcaa tcataagtca caatattcaa caaacatgag
    61801 aaactgtttt ttgaaattgt tccttgtgat tcagagttaa acgacagtct cacaacctct
    61861 tggtaaacgg cgttcaatca gctgaccctc ggtattagct caaaaaacgg taaaatatga
    61921 gaagctattt ttccgttttg tttctctaat acttaggata aaacgctgat tttacaacct
    61981 ctgttaaacg gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg
    62041 aggaactatt tttccgtttg tttctctaat actcaggaca aaacgctgat tttacaacct
    62101 cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    62161 atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
    62221 cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata aatgatacaa
    62281 catacactga taagaacaat ggcacctgca aaataaaacg catagctttc accaatatgt
    62341 tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg gacaaaacta
    62401 tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac
    62461 agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg tacaaggact
    62521 ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac aaatgctgct
    62581 aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca
    62641 atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc agaatagcca
    62701 atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg
    62761 attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc ccaaaggcct
    62821 acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa
    62881 aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc caagacccca
    62941 ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat catgattgct
    63001 ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga gacaaataaa
    63061 gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt cccagataat
    63121 ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt ttttttcaaa
    63181 ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac taacatgtaa
    63241 ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc aatcgttttt
    63301 aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc tgctacaact
    63361 agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc tatttgattc
    63421 tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt tacatgtcaa
    63481 tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata agacgagagg
    63541 taggattttt atgaatactt tagcctatat attattatgc gtgcttttat ccaaaccacg
    63601 ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc acagtcagat
    63661 ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg acaaagacga
    63721 gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg tagaggaatg
    63781 gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa aaatctatgc
    63841 tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag aacaacagct
    63901 gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag acaagaaaca
    63961 agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc aggaactcca
    64021 atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga taaacagaac
    64081 ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa tacagttgga
    64141 aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact atttttctag
    64201 aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa ataacagtgt
    64261 tccatcattc attactttga attgctttta gcacacttct tgaaagcggg ccaacgatga
    64321 tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata taagcagaga
    64381 ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata catgtcacca
    64441 ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt tctggattta
    64501 ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca tccaaaatga
    64561 aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga agatcttgat
    64621 gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc attgttgtaa
    64681 aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa tttgtcacga
    64741 aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt ctgccctttt
    64801 atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa
    64861 atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc catatcaata
    64921 aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag caaagcaatc
    64981 gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa agtttcctga
    65041 gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc tgtttgtctc
    65101 atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg ctgatatact
    65161 tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat atgttctaat
    65221 aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc taaagaaaac
    65281 tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac attttgtccc
    65341 atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg gatttgtttc
    65401 atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa tgttttcgca
    65461 ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa ccgatccagt
    65521 ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc ttgttttttc
    65581 ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc tgcttctcgt
    65641 agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt ccccgaaaac
    65701 tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc tagaaaatag
    65761 cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc aatcagtcga
    65821 tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc ttgttcataa
    65881 gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca tttccgctct
    65941 gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag gtcctttgct
    66001 tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg gattttccca
    66061 tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc gacaccattt
    66121 tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat agatagttct
    66181 agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt ttccatatgt
    66241 tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc
    66301 tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac tatgactaaa
    66361 tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc
    66421 catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt attcaaaagt
    66481 tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat taggttacca
    66541 cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt tattcgaaca
    66601 tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc gcttttccta
    66661 ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc tctgccaact
    66721 gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc gcaactaaca
    66781 tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt tcttctcttg
    66841 aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact tcttttggca
    66901 tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa cattgcatat
    66961 cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg aaacggatca
    67021 aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc catggtttca
    67081 tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg
    67141 ggattttgta tgtttcatca tatacgcgaa cggatcttct accagtcata attgttgaaa
    67201 aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat attgcaaagt
    67261 tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc gctgaaagcc
    67321 ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat tttcttctgg
    67381 gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct ctgacgcgaa
    67441 acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg atcaatctgc
    67501 aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct catcatgatc
    67561 tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat tttttgatcg
    67621 gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat ttcctccttt
    67681 ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa gaaaaaagtc
    67741 aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga gaaaagaaat
    67801 tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac caagattatt
    67861 gattcttatc aaactataga agatagctct tctgcagtag ataattttac accgccaaaa
    67921 atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga tcagtatgct
    67981 taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga gtaaacggtg
    68041 ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga aaatctgttt
    68101 ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc tttttaataa
    68161 ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc tctttcctta
    68221 gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag tcgataaatt
    68281 ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat ttttgttaaa
    68341 gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg attgtataaa
    68401 cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata ttcccagcct
    68461 acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc tcctgtatga
    68521 cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga
    68581 ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt ggcataacga
    68641 cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca tgcaccggaa
    68701 tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat
    68761 gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca ttgcccattc
    68821 acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt aatcccaaat
    68881 agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc aacgatggct
    68941 tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg tgcagttgag
    69001 gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata aaaagcatag
    69061 ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa tgtttgccca
    69121 tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc ttctgctgat
    69181 gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt
    69241 gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc aattcctgtt
    69301 tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc tagcactgtt
    69361 gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt ctttgctttc
    69421 atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac taaaaaagta
    69481 aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa tttgcttttt
    69541 ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa attgatggtc
    69601 gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg gattatactt
    69661 cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc gtttctatcg
    69721 caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt tgcgcttcat
    69781 gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt tgaacagctc
    69841 ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt aatccttcca
    69901 tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga taaacaacat
    69961 gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc atgattggaa
    70021 gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata ttttgccatt
    70081 catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta ttgtacaata
    70141 catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact tgtgcaaaat
    70201 ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct tttattgctt
    70261 ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct gctaatagct
    70321 tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact ttttctgata
    70381 aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact cactatctct
    70441 tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg gcagggattc
    70501 ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct gcaacaatgg
    70561 cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc caagcatttt
    70621 tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg gttagatggt
    70681 tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa cagatgccac
    70741 ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata ctaatatgtt
    70801 tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact ttgtcactaa
    70861 tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta ttcagcgctg
    70921 ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc ggatccgtta
    70981 ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg ctccctcctt
    71041 ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac atacttaact
    71101 attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa aaaatggata
    71161 ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa
    71221 aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta gaagaagaac
    71281 ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat tctggtcgat
    71341 atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa
    71401 caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact aaagcaatct
    71461 ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc cagtttcatt
    71521 tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta cttgattttg
    71581 gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg ccagcccaag
    71641 atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca gtcatccatc
    71701 cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg gacagtcaac
    71761 tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca tataacttat
    71821 tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct ctagatggga
    71881 tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca ttacaagctt
    71941 cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt aaatttttag
    72001 atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc agctttttaa
    72061 caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct taatttgttc
    72121 cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa gaattgacaa
    72181 aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat gtgactagat
    72241 catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt ttcgtgtcta
    72301 tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct tcaaaaaggg
    72361 tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag atgacggtgg
    72421 acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa atgatttgat
    72481 ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa tcaagttgca
    72541 aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga tcattgatca
    72601 tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca
    72661 cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca aacttttaag
    72721 agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag tagagtatct
    72781 gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg caatccatat
    72841 tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag tgacgattgt
    72901 atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt cagaaccatt
    72961 ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac gaacccatgc
    73021 gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa aatacccaga
    73081 aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc agctcgcgat
    73141 cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa gcgcaatcgt
    73201 acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct acaacgaatg
    73261 ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact agtagggatc
    73321 ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca agctggcggt
    73381 aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact agggatgtca
    73441 gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt tccagtaaat
    73501 gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc agttgatcca
    73561 tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat tgcggcagct
    73621 ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt tagcggcaag
    73681 cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt gctactgctc
    73741 gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt gagtttaggc
    73801 tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac aggattgcat
    73861 caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat tggtaaattt
    73921 ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt tttcccaatc
    73981 atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg tccagaaaac
    74041 aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt tactggtgta
    74101 acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt cttgcatgca
    74161 attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc aggatttgcc
    74221 tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc gaatcagcca
    74281 ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg ctttaacttt
    74341 gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct ttcagatgac
    74401 gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa aatctacgct
    74461 gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg ccttcgtttg
    74521 caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg cgtacctggt
    74581 atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt
    74641 gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca aacaaacacc
    74701 gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta cgctatcgca
    74761 aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga aaaaatgatg
    74821 ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat cgctggtacg
    74881 atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa
    74941 gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt cactttatat
    75001 gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga tcttgccgct
    75061 attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc
    75121 caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt
    75181 tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac aaacaggagc
    75241 tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc tcctgccaac
    75301 tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta tttgactctt
    75361 cttattactt gatgcagaac agctttttta caacctctga taaacggtgt tcaaaaacca
    75421 gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt
    75481 tcttcatact caaaactaat cggctttttc acaacctctt tatacattat tcctgatcca
    75541 atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact ttttctaaat
    75601 catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct aaaaacatgt
    75661 cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct gcatcacttg
    75721 cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct aattcttcat
    75781 agtcagttag attgccggat aattctaaga tttctggaga gtcactttct actagtactt
    75841 ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca tttcctgcag
    75901 cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca ttctctacat
    75961 accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt ttttcagtga
    76021 attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa cttgtataag
    76081 cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt tcggcttgac
    76141 gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac gcttctttca
    76201 ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct ttcagtgctt
    76261 ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc atctttttta
    76321 agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa taatgcatat
    76381 ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta taaattacac
    76441 ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg atgacaactg
    76501 atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat tgaaatgttt
    76561 caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac aaatgcagca
    76621 aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac aaaaaccgga
    76681 aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga tgtctatcgc
    76741 agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc
    76801 gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca gaaaaaggca
    76861 gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc cgttttggaa
    76921 aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt ctctctactg
    76981 tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc gggggacaaa
    77041 agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga agtcatgacg
    77101 tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa atgcaaaata
    77161 tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta ctttcacgac
    77221 ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata tatttcccgt
    77281 tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa tcttctactt
    77341 ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct tcaggcgatt
    77401 ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt cgggtagtgt
    77461 ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg ttatagggtt
    77521 cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct attgttccat
    77581 taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca aaatccgtat
    77641 tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc
    77701 tatactttgc catttctccc accctttcta ttatattaag ttcatcttac ataaaattaa
    77761 agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt aactattttt
    77821 ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct ccccattttg
    77881 taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc cattctgcat
    77941 agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg
    78001 cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt tcttttgtcg
    78061 tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct ccatcaaatc
    78121 cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc atctcttccc
    78181 tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact ccttcaaatt
    78241 tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt tcgatacgaa
    78301 attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga aaagattttt
    78361 gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga aaagctcttg
    78421 aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact cttgatccat
    78481 ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc attagcgaca
    78541 tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct gtattttcgg
    78601 ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg atcaattggt
    78661 tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata ttatcaaaga
    78721 aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat gttgtatgga
    78781 tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc tgcctatcta
    78841 tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa caaagcggta
    78901 aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa gcaaattgtt
    78961 gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc atacctcttt
    79021 tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc agataaataa
    79081 a
//
LOCUS       DANJJW010000004        72424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000004 DANJJW010000000
VERSION     DANJJW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(395..1330)
                     /gene="murB"
                     /locus_tag="PWS82_000241"
     CDS             complement(395..1330)
                     /gene="murB"
                     /locus_tag="PWS82_000241"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL0905401.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1403..2323)
                     /locus_tag="PWS82_000242"
     CDS             complement(1403..2323)
                     /locus_tag="PWS82_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0905402.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2436..3188
                     /gene="xth"
                     /locus_tag="PWS82_000243"
     CDS             2436..3188
                     /gene="xth"
                     /locus_tag="PWS82_000243"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL0905403.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4052..4585
                     /locus_tag="PWS82_000244"
     CDS             4052..4585
                     /locus_tag="PWS82_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL0905404.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(5075..5599)
                     /locus_tag="PWS82_000245"
     CDS             complement(5075..5599)
                     /locus_tag="PWS82_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905405.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5592..6065)
                     /gene="tsaE"
                     /locus_tag="PWS82_000246"
     CDS             complement(5592..6065)
                     /gene="tsaE"
                     /locus_tag="PWS82_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL0905406.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6188..7168)
                     /gene="pta"
                     /locus_tag="PWS82_000247"
     CDS             complement(6188..7168)
                     /gene="pta"
                     /locus_tag="PWS82_000247"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL0905407.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7321..8001)
                     /locus_tag="PWS82_000248"
     CDS             complement(7321..8001)
                     /locus_tag="PWS82_000248"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0905408.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            8127..8996
                     /locus_tag="PWS82_000249"
     CDS             8127..8996
                     /locus_tag="PWS82_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0905409.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            9022..9537
                     /locus_tag="PWS82_000250"
     CDS             9022..9537
                     /locus_tag="PWS82_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905410.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9584..10177)
                     /locus_tag="PWS82_000251"
     CDS             complement(9584..10177)
                     /locus_tag="PWS82_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905411.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10202..10381)
                     /locus_tag="PWS82_000252"
     CDS             complement(10202..10381)
                     /locus_tag="PWS82_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905412.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10458..10967)
                     /locus_tag="PWS82_000253"
     CDS             complement(10458..10967)
                     /locus_tag="PWS82_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905413.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(11063..11443)
                     /locus_tag="PWS82_000254"
     CDS             complement(11063..11443)
                     /locus_tag="PWS82_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0905414.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11773..12522
                     /locus_tag="PWS82_000255"
     CDS             11773..12522
                     /locus_tag="PWS82_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL0905415.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12706..13983)
                     /locus_tag="PWS82_000256"
     CDS             complement(12706..13983)
                     /locus_tag="PWS82_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905416.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(14062..14706)
                     /gene="pcp"
                     /locus_tag="PWS82_000257"
     CDS             complement(14062..14706)
                     /gene="pcp"
                     /locus_tag="PWS82_000257"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL0905417.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14905..15849)
                     /locus_tag="PWS82_000258"
     CDS             complement(14905..15849)
                     /locus_tag="PWS82_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL0905418.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15851..16537)
                     /locus_tag="PWS82_000259"
     CDS             complement(15851..16537)
                     /locus_tag="PWS82_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL0905419.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17011..17331
                     /locus_tag="PWS82_000260"
     CDS             17011..17331
                     /locus_tag="PWS82_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL0905420.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17485..18390
                     /locus_tag="PWS82_000261"
     CDS             17485..18390
                     /locus_tag="PWS82_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905421.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18387..19160
                     /locus_tag="PWS82_000262"
     CDS             18387..19160
                     /locus_tag="PWS82_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0905422.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19341..20765)
                     /locus_tag="PWS82_000263"
     CDS             complement(19341..20765)
                     /locus_tag="PWS82_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0905423.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20976..21422
                     /gene="nrdI"
                     /locus_tag="PWS82_000264"
     CDS             20976..21422
                     /gene="nrdI"
                     /locus_tag="PWS82_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0905424.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21487..23469)
                     /locus_tag="PWS82_000265"
     CDS             complement(21487..23469)
                     /locus_tag="PWS82_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0905425.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23483..24379)
                     /gene="murQ"
                     /locus_tag="PWS82_000266"
     CDS             complement(23483..24379)
                     /gene="murQ"
                     /locus_tag="PWS82_000266"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0905426.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24382..25443)
                     /locus_tag="PWS82_000267"
     CDS             complement(24382..25443)
                     /locus_tag="PWS82_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0905427.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25654..26220)
                     /locus_tag="PWS82_000268"
     CDS             complement(25654..26220)
                     /locus_tag="PWS82_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0905428.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(26248..27750)
                     /gene="eat(A)"
                     /locus_tag="PWS82_000269"
     CDS             complement(26248..27750)
                     /gene="eat(A)"
                     /locus_tag="PWS82_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0905429.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(28008..29129)
                     /locus_tag="PWS82_000270"
     CDS             complement(28008..29129)
                     /locus_tag="PWS82_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0905430.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            29255..30196
                     /locus_tag="PWS82_000271"
     CDS             29255..30196
                     /locus_tag="PWS82_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0905431.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(30346..31242)
                     /locus_tag="PWS82_000272"
     CDS             complement(30346..31242)
                     /locus_tag="PWS82_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0905432.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31511..32851
                     /locus_tag="PWS82_000273"
     CDS             31511..32851
                     /locus_tag="PWS82_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0905433.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            33069..33512
                     /locus_tag="PWS82_000274"
     CDS             33069..33512
                     /locus_tag="PWS82_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL0905434.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(34016..35434)
                     /gene="nhaC"
                     /locus_tag="PWS82_000275"
     CDS             complement(34016..35434)
                     /gene="nhaC"
                     /locus_tag="PWS82_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0905435.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(35837..37060)
                     /locus_tag="PWS82_000276"
     CDS             complement(35837..37060)
                     /locus_tag="PWS82_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL0905436.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(37091..37930)
                     /locus_tag="PWS82_000277"
     CDS             complement(37091..37930)
                     /locus_tag="PWS82_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0905437.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(37947..38627)
                     /locus_tag="PWS82_000278"
     CDS             complement(37947..38627)
                     /locus_tag="PWS82_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905438.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(38629..39702)
                     /locus_tag="PWS82_000279"
     CDS             complement(38629..39702)
                     /locus_tag="PWS82_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905439.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(39758..39996)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(40039..40272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(40349..41020)
                     /locus_tag="PWS82_000280"
     CDS             complement(40349..41020)
                     /locus_tag="PWS82_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0905440.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(41107..43785)
                     /gene="mgtA"
                     /locus_tag="PWS82_000281"
     CDS             complement(41107..43785)
                     /gene="mgtA"
                     /locus_tag="PWS82_000281"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL0905441.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44444..44881
                     /gene="ndk"
                     /locus_tag="PWS82_000282"
     CDS             44444..44881
                     /gene="ndk"
                     /locus_tag="PWS82_000282"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL0905442.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(44903..45769)
                     /locus_tag="PWS82_000283"
     CDS             complement(44903..45769)
                     /locus_tag="PWS82_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0905443.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(45929..48022)
                     /locus_tag="PWS82_000284"
     CDS             complement(45929..48022)
                     /locus_tag="PWS82_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0905444.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(48771..49394)
                     /locus_tag="PWS82_000285"
     CDS             complement(48771..49394)
                     /locus_tag="PWS82_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905445.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(49553..50950)
                     /locus_tag="PWS82_000286"
     CDS             complement(49553..50950)
                     /locus_tag="PWS82_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0905446.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(50982..51953)
                     /locus_tag="PWS82_000287"
     CDS             complement(50982..51953)
                     /locus_tag="PWS82_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0905447.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            52327..52752
                     /locus_tag="PWS82_000288"
     CDS             52327..52752
                     /locus_tag="PWS82_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905448.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(53240..53941)
                     /locus_tag="PWS82_000289"
     CDS             complement(53240..53941)
                     /locus_tag="PWS82_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0905449.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(53957..54397)
                     /locus_tag="PWS82_000290"
     CDS             complement(53957..54397)
                     /locus_tag="PWS82_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0905450.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(54397..54972)
                     /locus_tag="PWS82_000291"
     CDS             complement(54397..54972)
                     /locus_tag="PWS82_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL0905451.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(55820..56299)
                     /locus_tag="PWS82_000292"
                     /pseudo
     CDS             complement(55820..56299)
                     /locus_tag="PWS82_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(56458..57159)
                     /locus_tag="PWS82_000293"
     CDS             complement(56458..57159)
                     /locus_tag="PWS82_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0905452.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(57267..58676)
                     /locus_tag="PWS82_000294"
     CDS             complement(57267..58676)
                     /locus_tag="PWS82_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL0905453.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(58724..60589)
                     /locus_tag="PWS82_000295"
     CDS             complement(58724..60589)
                     /locus_tag="PWS82_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0905454.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(60710..61561)
                     /locus_tag="PWS82_000296"
     CDS             complement(60710..61561)
                     /locus_tag="PWS82_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0905455.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(61871..62584)
                     /locus_tag="PWS82_000297"
     CDS             complement(61871..62584)
                     /locus_tag="PWS82_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL0905456.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(62652..62978)
                     /locus_tag="PWS82_000298"
     CDS             complement(62652..62978)
                     /locus_tag="PWS82_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0905457.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(63101..63328)
                     /locus_tag="PWS82_000299"
     CDS             complement(63101..63328)
                     /locus_tag="PWS82_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0905458.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(63650..64903)
                     /locus_tag="PWS82_000300"
     CDS             complement(63650..64903)
                     /locus_tag="PWS82_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0905459.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            64964..65704
                     /locus_tag="PWS82_000301"
     CDS             64964..65704
                     /locus_tag="PWS82_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0905460.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(65790..66548)
                     /locus_tag="PWS82_000302"
     CDS             complement(65790..66548)
                     /locus_tag="PWS82_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0905461.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(66545..67573)
                     /locus_tag="PWS82_000303"
                     /pseudo
     CDS             complement(66545..67573)
                     /locus_tag="PWS82_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(67593..70760)
                     /locus_tag="PWS82_000304"
     CDS             complement(67593..70760)
                     /locus_tag="PWS82_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905462.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(70984..71868)
                     /locus_tag="PWS82_000305"
     CDS             complement(70984..71868)
                     /locus_tag="PWS82_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0905463.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(71963..72400)
                     /locus_tag="PWS82_000306"
     CDS             complement(71963..72400)
                     /locus_tag="PWS82_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905464.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        20830 a        15178 c        12521 g        23895 t
ORIGIN      
        1 taggcagcac tacataattc tttttctcaa attctaaata tctagctagc agcagttttt
       61 ctctatcttc ctcttctata aggtaatcca acttcatatc ctacacctca aaaaatgcct
      121 cataccaatt acttgatatg cagatgaaag ttcatctttt cacctatact gattttatga
      181 tggattatgc ttgttttttc atcagttatt ctcatttttt atcttttttt tgagaatatt
      241 gcaaaaaatg gaaaaattta tgaggaaata ttctatattt tttgttttat tgtgttaaaa
      301 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
      361 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      421 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      481 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      541 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      601 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      661 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      721 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      781 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      841 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      901 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      961 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
     1021 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
     1081 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
     1141 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
     1201 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
     1261 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1321 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1381 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1441 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1501 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1561 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcctcttga atgacaagct
     1621 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1681 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1741 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1801 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1861 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1921 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1981 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     2041 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     2101 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     2161 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     2221 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     2281 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2341 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2401 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2461 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2521 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2581 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2641 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2701 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2761 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2821 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2881 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2941 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     3001 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     3061 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     3121 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     3181 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     3241 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3301 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3361 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3421 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3481 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3541 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3601 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3661 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3721 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     3781 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     3841 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     3901 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     3961 atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt
     4021 tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac
     4081 tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca
     4141 gatcgttgat gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt
     4201 ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg
     4261 gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt
     4321 tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct
     4381 ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt
     4441 gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga
     4501 tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt
     4561 gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag
     4621 cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt
     4681 aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt
     4741 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     4801 ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt accaagaggt
     4861 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggcaaaata gttcctcata
     4921 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt tattcaaaaa
     4981 aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg ccttctcaaa
     5041 aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa ctcattttat
     5101 atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc gcttcgattt
     5161 ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga acgtcaagct
     5221 ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct agcataagag
     5281 tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct cccacgtgag
     5341 acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc ccttcataga
     5401 ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct tcttctaagg
     5461 tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc tccttattaa
     5521 cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc cgcaagatga
     5581 tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg tttgaaggtt
     5641 tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc tgtatcactt
     5701 ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag atttccccat
     5761 tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc tgctccgcta
     5821 gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact atattctcta
     5881 ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg ggcaattcct
     5941 ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt gtcacctggc
     6001 attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc tgatagttcg
     6061 atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg taatgaagtt
     6121 tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc tagttcgcgt
     6181 gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta tatacatctt
     6241 cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt agaattggtc
     6301 cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt cctgactgca
     6361 attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc gctttcagtt
     6421 gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc atgtattgtg
     6481 gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct ggcgatttcg
     6541 ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg aacaattctg
     6601 ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga ttcacgttga
     6661 tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg atcatcgctc
     6721 cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg cgaacagtgt
     6781 cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata tacgtaagca
     6841 tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg gatgcctttc
     6901 catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa ttatcagggc
     6961 tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt cttatttctt
     7021 cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg gcagctgcgc
     7081 ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg cgaacaattt
     7141 taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt atatgtatta
     7201 tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc aaaaatgctg
     7261 gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag cgctgtttta
     7321 ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa ggagcgcatc
     7381 attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata acggactggg
     7441 atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt tttcttgtgc
     7501 cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt tttcaatgat
     7561 cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc cttcccgaac
     7621 ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta gatttccatg
     7681 ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc ttaacgatgg
     7741 cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat tttctccatg
     7801 ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca attccaatgc
     7861 ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt tttttaaaaa
     7921 ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt cttgccaact
     7981 gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac tcacattttc
     8041 ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa actaggaaca
     8101 agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat atggacggca
     8161 cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata cgttttgcaa
     8221 atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa gctcgcgcag
     8281 ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca caggtatttg
     8341 ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca atctctgttt
     8401 tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat ggtctttatt
     8461 ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc catttacctc
     8521 accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta ttgcacatca
     8581 catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt ttaaaaatta
     8641 tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag atttctcttt
     8701 taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac cataaaaacg
     8761 ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct ttagaagaag
     8821 tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca ggagtcagtt
     8881 tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca acggacacaa
     8941 accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac ttgtagtttt
     9001 atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca gcttggacgt
     9061 gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat ggttggtcgc
     9121 tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct cgttgctcac
     9181 atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga gttcgaaaag
     9241 gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat tcgtagactt
     9301 cactggacag tccaaggtga tatctataat catcaatatc aaatccatca tttcaatcag
     9361 caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt attggatgtc
     9421 tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga ttactggctc
     9481 taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt aagttaaaaa
     9541 gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata ttcttttgca
     9601 tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag tcatggcgtt
     9661 cgtataattt tttagccttt ggattttttt catccacact tagaccgatg acggagcgat
     9721 tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg ccttgtcctc
     9781 taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac gtttctttat
     9841 caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt accagtggct
     9901 gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca actttaccgt
     9961 ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca gtgattgctt
    10021 cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc agttccatat
    10081 cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt ctcgcaaaac
    10141 gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa tcgcaaaatc
    10201 gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta acgattcttg
    10261 ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta tgttcgacta
    10321 cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc attttttcca
    10381 tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt gtaaaaaaga
    10441 gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc tccatctatt
    10501 aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc tttcactttt
    10561 gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg tttgaactct
    10621 tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt tcgtacggtt
    10681 gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa ctggtattca
    10741 tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat agcagatatg
    10801 ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga gatccgatat
    10861 tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt cataccggta
    10921 atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct tcattctcct
    10981 gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat gaaacgaaaa
    11041 aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg tctggatcaa
    11101 agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct gattcgatcc
    11161 ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa gcaagatgac
    11221 gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt ccaaaaatct
    11281 ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct ttgcggtaat
    11341 tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat ttctgataat
    11401 ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca ctcctattct
    11461 tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc agattgaaaa
    11521 aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag taggtcctcc
    11581 aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat tttcaagaaa
    11641 acaaacagaa aattatcata acacaaatta tttataattg aaagactagt tttttttcaa
    11701 gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta gtgtcttaag
    11761 gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa aggctggccg
    11821 caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac tcttgtcaat
    11881 agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg tgtattgtgt
    11941 attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt gtctgcgatt
    12001 tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga acagattgct
    12061 tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca taatatggct
    12121 tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag tactttcctt
    12181 gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg gccgtgggta
    12241 gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt gcgttacaac
    12301 aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg gtatatttca
    12361 tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta cttgctgaat
    12421 gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga acgtatgcaa
    12481 aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg agcgtgggac
    12541 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aatcagaagc
    12601 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12661 ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata gtaattcctt
    12721 cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct gataatattc
    12781 caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt tgtcgattgt
    12841 ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt cttttgagac
    12901 tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca atacttttaa
    12961 aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa aaccatagcg
    13021 acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt ggtttttcgc
    13081 aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct ttctcaacac
    13141 ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat atacgatgga
    13201 ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta catcctttac
    13261 taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt ttgactgccc
    13321 attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat ccgctatgta
    13381 aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta tatccggtaa
    13441 atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt gccatagtcc
    13501 ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg ccagatcaac
    13561 ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca ggtgatcggc
    13621 aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa cgatgtattg
    13681 cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt ttgttcgaaa
    13741 aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat aatgttcttc
    13801 cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc gctgtttttt
    13861 cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag aaattttgat
    13921 gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt gccattcttc
    13981 cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa cgttagcaac
    14041 cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa tatcttcttt
    14101 attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt ctaaagacat
    14161 agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca cgtggatgaa
    14221 cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat acatgatatc
    14281 attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat gatcttgtac
    14341 attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac cgttttcttc
    14401 taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt ctgctagatt
    14461 gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga cacagatcac
    14521 gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt tttcgaatac
    14581 ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca ttttttttac
    14641 tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg ttactagtac
    14701 tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt atcataccat
    14761 tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca gatcaaactt
    14821 aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt gcgacaatta
    14881 ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa tttggaaaac
    14941 tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt ctttcatctc
    15001 cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca gggtcccaca
    15061 aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat gactaaatac
    15121 gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat tccccatgat
    15181 taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca catttccttc
    15241 tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc ttgtaaatac
    15301 cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg tgcccagcat
    15361 atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga acatcaagat
    15421 tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat tggtaaatag
    15481 agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt tcattcctag
    15541 tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc ctttgctgac
    15601 tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca cgattgccgg
    15661 aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg ctgacccata
    15721 acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca atacgagtcc
    15781 catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag gatttgtaaa
    15841 aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc ttttctttaa
    15901 gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact tccactaaag
    15961 caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg actagcaacg
    16021 cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt tccatagctg
    16081 aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct tttgattcga
    16141 tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact ccaccaaaac
    16201 tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca atcataccag
    16261 cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga ccatttcttt
    16321 caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga ttgtttacaa
    16381 aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc aaagctgtaa
    16441 caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac ccgagaataa
    16501 caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca taaaatgaca
    16561 atattttttt aattataaag caaaacacat aaaaaatata gttgttttct cacgaaatag
    16621 acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt acttattgta
    16681 aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc tagttactgt
    16741 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
    16801 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
    16861 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
    16921 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
    16981 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
    17041 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
    17101 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
    17161 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
    17221 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
    17281 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
    17341 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
    17401 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
    17461 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
    17521 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
    17581 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
    17641 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
    17701 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
    17761 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
    17821 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
    17881 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
    17941 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
    18001 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
    18061 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
    18121 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
    18181 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
    18241 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
    18301 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
    18361 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
    18421 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
    18481 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
    18541 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
    18601 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
    18661 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
    18721 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
    18781 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
    18841 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
    18901 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
    18961 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
    19021 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
    19081 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
    19141 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
    19201 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
    19261 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
    19321 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
    19381 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
    19441 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
    19501 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
    19561 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
    19621 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
    19681 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
    19741 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
    19801 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
    19861 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
    19921 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
    19981 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
    20041 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
    20101 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
    20161 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
    20221 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
    20281 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
    20341 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
    20401 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
    20461 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
    20521 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
    20581 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
    20641 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
    20701 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
    20761 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
    20821 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
    20881 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
    20941 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
    21001 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
    21061 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
    21121 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
    21181 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
    21241 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
    21301 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
    21361 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
    21421 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
    21481 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
    21541 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
    21601 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
    21661 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
    21721 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
    21781 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
    21841 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
    21901 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
    21961 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
    22021 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
    22081 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
    22141 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
    22201 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
    22261 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
    22321 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
    22381 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
    22441 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
    22501 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
    22561 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
    22621 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
    22681 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
    22741 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
    22801 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
    22861 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
    22921 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
    22981 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
    23041 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
    23101 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
    23161 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
    23221 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
    23281 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
    23341 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
    23401 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
    23461 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
    23521 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
    23581 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
    23641 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
    23701 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
    23761 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
    23821 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
    23881 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
    23941 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
    24001 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
    24061 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
    24121 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
    24181 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
    24241 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
    24301 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
    24361 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
    24421 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
    24481 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
    24541 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
    24601 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
    24661 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
    24721 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
    24781 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
    24841 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
    24901 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
    24961 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
    25021 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
    25081 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
    25141 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
    25201 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
    25261 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
    25321 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
    25381 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
    25441 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
    25501 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
    25561 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
    25621 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
    25681 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
    25741 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
    25801 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
    25861 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
    25921 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
    25981 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
    26041 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
    26101 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
    26161 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
    26221 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
    26281 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
    26341 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
    26401 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
    26461 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
    26521 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
    26581 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
    26641 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
    26701 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    26761 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    26821 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    26881 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    26941 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    27001 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    27061 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    27121 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    27181 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    27241 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    27301 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    27361 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    27421 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    27481 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    27541 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    27601 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    27661 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    27721 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    27781 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    27841 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    27901 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    27961 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    28021 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    28081 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    28141 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    28201 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    28261 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    28321 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    28381 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    28441 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    28501 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    28561 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    28621 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    28681 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    28741 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    28801 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    28861 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    28921 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    28981 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    29041 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    29101 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    29161 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    29221 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    29281 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    29341 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    29401 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    29461 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    29521 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    29581 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    29641 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    29701 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    29761 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    29821 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    29881 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    29941 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    30001 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    30061 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    30121 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    30181 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    30241 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    30301 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    30361 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    30421 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    30481 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    30541 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    30601 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    30661 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    30721 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    30781 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    30841 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    30901 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    30961 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    31021 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    31081 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    31141 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    31201 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    31261 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    31321 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    31381 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    31441 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    31501 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    31561 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    31621 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    31681 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    31741 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    31801 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    31861 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    31921 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    31981 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    32041 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    32101 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    32161 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    32221 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    32281 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    32341 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    32401 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    32461 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    32521 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    32581 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    32641 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    32701 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    32761 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    32821 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    32881 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    32941 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    33001 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    33061 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    33121 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    33181 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    33241 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    33301 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    33361 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    33421 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    33481 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    33541 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    33601 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    33661 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    33721 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    33781 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    33841 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    33901 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    33961 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    34021 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    34081 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    34141 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    34201 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    34261 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    34321 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    34381 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    34441 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    34501 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    34561 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    34621 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    34681 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    34741 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    34801 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    34861 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    34921 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    34981 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    35041 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    35101 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    35161 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    35221 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    35281 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    35341 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    35401 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    35461 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    35521 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    35581 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    35641 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    35701 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    35761 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    35821 acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    35881 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    35941 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    36001 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    36061 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    36121 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    36181 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    36241 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    36301 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    36361 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    36421 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    36481 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    36541 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    36601 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    36661 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    36721 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    36781 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    36841 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    36901 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    36961 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    37021 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    37081 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    37141 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    37201 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    37261 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    37321 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    37381 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    37441 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    37501 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    37561 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    37621 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    37681 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    37741 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    37801 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    37861 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    37921 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    37981 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    38041 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    38101 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    38161 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    38221 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    38281 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    38341 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    38401 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    38461 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    38521 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    38581 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    38641 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    38701 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    38761 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    38821 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    38881 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    38941 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    39001 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    39061 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    39121 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    39181 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    39241 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    39301 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    39361 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    39421 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    39481 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    39541 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    39601 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    39661 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    39721 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    39781 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    39841 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    39901 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    39961 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    40021 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    40081 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    40141 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    40201 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    40261 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    40321 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    40381 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    40441 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    40501 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    40561 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    40621 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    40681 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    40741 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    40801 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    40861 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    40921 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    40981 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    41041 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    41101 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    41161 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    41221 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    41281 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    41341 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    41401 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    41461 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    41521 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    41581 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    41641 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    41701 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    41761 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    41821 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    41881 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    41941 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    42001 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    42061 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    42121 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    42181 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    42241 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    42301 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    42361 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    42421 cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    42481 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    42541 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    42601 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    42661 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    42721 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    42781 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    42841 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    42901 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    42961 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    43021 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    43081 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    43141 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    43201 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    43261 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    43321 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    43381 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    43441 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    43501 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    43561 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    43621 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    43681 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    43741 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    43801 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    43861 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    43921 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    43981 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    44041 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    44101 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    44161 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    44221 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    44281 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    44341 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    44401 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    44461 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    44521 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    44581 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    44641 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    44701 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    44761 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    44821 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    44881 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    44941 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    45001 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    45061 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    45121 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    45181 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    45241 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    45301 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    45361 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    45421 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    45481 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    45541 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    45601 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    45661 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    45721 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    45781 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    45841 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    45901 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    45961 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    46021 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    46081 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    46141 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    46201 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    46261 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    46321 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    46381 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    46441 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    46501 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    46561 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    46621 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    46681 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    46741 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    46801 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    46861 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    46921 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    46981 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    47041 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    47101 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    47161 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    47221 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    47281 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    47341 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    47401 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    47461 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    47521 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    47581 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    47641 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    47701 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    47761 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    47821 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    47881 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    47941 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    48001 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    48061 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    48121 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    48181 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    48241 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    48301 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    48361 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    48421 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    48481 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    48541 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    48601 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    48661 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    48721 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    48781 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    48841 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    48901 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    48961 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    49021 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    49081 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    49141 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    49201 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    49261 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    49321 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    49381 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    49441 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    49501 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    49561 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    49621 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    49681 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    49741 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    49801 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    49861 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    49921 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    49981 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    50041 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    50101 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    50161 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    50221 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    50281 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    50341 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    50401 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    50461 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    50521 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    50581 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    50641 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    50701 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    50761 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    50821 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    50881 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    50941 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    51001 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    51061 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    51121 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    51181 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    51241 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    51301 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    51361 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    51421 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    51481 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    51541 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    51601 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    51661 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    51721 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    51781 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    51841 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    51901 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    51961 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    52021 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    52081 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    52141 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    52201 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    52261 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    52321 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    52381 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    52441 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    52501 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    52561 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    52621 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    52681 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    52741 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    52801 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    52861 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    52921 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    52981 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    53041 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    53101 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    53161 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc
    53221 gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa
    53281 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca
    53341 ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt
    53401 taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat
    53461 aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac
    53521 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg
    53581 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    53641 cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga
    53701 tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    53761 tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg
    53821 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    53881 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    53941 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa
    54001 aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca
    54061 tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg
    54121 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    54181 caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc
    54241 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    54301 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    54361 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    54421 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    54481 gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    54541 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt
    54601 cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc
    54661 agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt
    54721 ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt
    54781 cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    54841 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc
    54901 taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac
    54961 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    55021 tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat agacgccact
    55081 atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa caaaaataaa
    55141 taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga tttcgtccta
    55201 tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat taacgaaaaa
    55261 atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa taaaaaagcc
    55321 gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct aaaaatcttt
    55381 ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac tgctggaaga
    55441 aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga tattgggctc
    55501 gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat aataactttc
    55561 tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga cataaggtat
    55621 accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt tcaaggaggg
    55681 tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt
    55741 ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta cttttttctt
    55801 gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg tcttcttcct
    55861 ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat aataaaatgg
    55921 ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat ggtctggtca
    55981 tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc caaaacatgc
    56041 ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt gccaagtcgt
    56101 tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga aaagcggtct
    56161 tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc cctaagaaat
    56221 aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca gtgattggtt
    56281 catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg gtaatccaga
    56341 aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga agctattttt
    56401 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tttttattta
    56461 tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct ccgaatattc
    56521 aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc
    56581 tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat catcagaaaa
    56641 tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt tgccaaattc
    56701 ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa tataaggctg
    56761 attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa
    56821 aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc ggacttcata
    56881 tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat tgtaaggacg
    56941 attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat ctgatgccag
    57001 cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt ctaattcact
    57061 ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt ctttaatttt
    57121 atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct ttgctttact
    57181 attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat gtgagaagtg
    57241 tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc aattacttct
    57301 ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc ttcatcgtct
    57361 agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc actgacaaga
    57421 tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac tgcattagcc
    57481 atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat cgtgccatcc
    57541 tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg tttctggtaa
    57601 cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg ccagccccat
    57661 tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc tgaagttgct
    57721 gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc gatgtagtcc
    57781 actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt ttccggatcg
    57841 actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg tacttggaca
    57901 tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg attgactgga
    57961 ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg gatatccgaa
    58021 cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt atgccatgct
    58081 tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg acttaaagct
    58141 ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac tttactgctg
    58201 tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt tcgatttttc
    58261 cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca
    58321 ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc taatcccgct
    58381 tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat cgaaaaacga
    58441 taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga
    58501 tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca gtcaaattct
    58561 tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc aacggatagt
    58621 ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg ctccacccca
    58681 taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt acaattgcca
    58741 gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg gcaaatgcat
    58801 cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta attgcttcga
    58861 tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg actaagatct
    58921 caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa atctccgctc
    58981 cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg atctctccgt
    59041 ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt cccatcacgc
    59101 cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc gttaaaggac
    59161 tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt ttgttttcta
    59221 attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa
    59281 caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca ccatccaccg
    59341 tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa gcttttactc
    59401 cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca atgaatggtt
    59461 tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat aacgaagtca
    59521 ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta
    59581 aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat acatcaaacc
    59641 catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca
    59701 cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta aatccataaa
    59761 tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt cctaaagcaa
    59821 gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc atttgcagaa
    59881 attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat acggctagaa
    59941 tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg atcccaaaga
    60001 aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca agtgccatag
    60061 caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta aaagcaagca
    60121 tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga taagtaccgc
    60181 tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc aataccccgg
    60241 cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg atcagacgat
    60301 tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct tcctcatcca
    60361 cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac tgaccgccac
    60421 tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct gctaaagatt
    60481 catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg acattctttt
    60541 gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt gttttttcct
    60601 tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca caaacattcg
    60661 tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct tatgattgga
    60721 tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc tgctctgcga
    60781 tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg acacaatgaa
    60841 acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc
    60901 cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata cgctgataat
    60961 tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc agtatatctc
    61021 ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt tggctattca
    61081 caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc gtcttttgtt
    61141 tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat ttttttgttt
    61201 caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc gcataatgaa
    61261 gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt ttatcttcag
    61321 ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca gccatcagct
    61381 gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg accgcttctg
    61441 gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata caaacgactt
    61501 cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta tcaattttca
    61561 ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact gtttcttagc
    61621 tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa atcatcatac
    61681 catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact ggaacacaag
    61741 tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt tttttcagca
    61801 aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta acgacttatg
    61861 tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata tcatctgtcg
    61921 catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc gtgacggcgt
    61981 ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt
    62041 gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac acattttcta
    62101 aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta aacagcaatt
    62161 cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt tcttgatctg
    62221 gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg aaaaccggta
    62281 aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct gttcctctca
    62341 tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag tcaaaactga
    62401 catctttatc gttattcttt ttcagatctt cttttgttac attcgctata gcatatttat
    62461 ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa gctagtccta
    62521 ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt cctttttttt
    62581 tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc caacagccga
    62641 atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt ttctacagga
    62701 tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc caggtgaagg
    62761 ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt gattcgttct
    62821 aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa caattcagat
    62881 gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg ccaattacgt
    62941 tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc ttccaatcac
    63001 ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc ttttaaaaaa
    63061 actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt aggtagccac
    63121 catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg atacgttctt
    63181 cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct tcggtgagcg
    63241 agtactctac tttaggcggg acttggttat aggcttttcg atggatactt tcatcttgtt
    63301 ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt tgcttgcttc
    63361 tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca aagcagttag
    63421 cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc atgaagtata
    63481 ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta catagcaaaa
    63541 gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt
    63601 gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt cagtaggctg
    63661 atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc tgttttgaaa
    63721 attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca ctgtatagac
    63781 ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat agcgtcaaat
    63841 tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct tccagcagat
    63901 tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa ataaaaatag
    63961 ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg acaggaataa
    64021 ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa aagacacccc
    64081 tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca aaaatagctg
    64141 cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt tcctgaagag
    64201 taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct agatgatggt
    64261 aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt tcttttactt
    64321 ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact atcgacaaaa
    64381 gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc aagtaaatca
    64441 cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga taagtaaaaa
    64501 gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag aaaataaatg
    64561 cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa taatccatca
    64621 gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta ctgatcaaac
    64681 agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat aagccaacta
    64741 ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc aaagctatac
    64801 aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata cctaaatcta
    64861 aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc ctccattgtt
    64921 ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa agtattttat
    64981 catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca atcatcatat
    65041 cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc agttgggcac
    65101 tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac tcacaggtat
    65161 tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt ttttcaccgc
    65221 ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg gatatatcag
    65281 aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca attggaaaga
    65341 acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa gtctcgtcct
    65401 gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc aagttatggt
    65461 caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga agacagagtt
    65521 agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat cgcaattgca
    65581 gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct tactcgttgg
    65641 tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa ctgcttcgag
    65701 ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc cttgaatcgg
    65761 ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg agcatctgtt
    65821 aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac aggaacaaat
    65881 aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat ccatgtgcca
    65941 atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg gatttcgatt
    66001 ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt tgcgctattt
    66061 cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt atcagatgtt
    66121 gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt tccgctttta
    66181 tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc cgttccgctt
    66241 tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa atcaagatct
    66301 ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc aggtgtaaaa
    66361 ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc aggatttgca
    66421 gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt attctcagca
    66481 aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa tgcaattgtt
    66541 tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat aagccaataa
    66601 gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga ttttttaaat
    66661 caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa tcggtatgga
    66721 gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt cccggagcta
    66781 atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct acatctattt
    66841 tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt tcaaccgatg
    66901 tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta atcacatttc
    66961 ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa gactcttttt
    67021 cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca ccaactgtac
    67081 ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg accacaccag
    67141 ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt tgaggaggaa
    67201 cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat gtttcatcaa
    67261 atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta gcggagtgga
    67321 tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag gggatttcct
    67381 gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca atctgattag
    67441 ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt gtctgccaaa
    67501 ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga cttttattcc
    67561 atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc tgctaatgtc
    67621 catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa gacaagttta
    67681 aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt tgaattttgt
    67741 cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc tttcttgaaa
    67801 tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga taattttatc
    67861 gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt ttcctgtata
    67921 tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc aaaatcgagt
    67981 ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc aggtgcaaaa
    68041 ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc aatttcatcg
    68101 ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg gtcgtttaat
    68161 acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag aacaaattcc
    68221 cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa tgaaaccaag
    68281 gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa tgtggtactt
    68341 aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc cagttctgtc
    68401 acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc ctccgttttt
    68461 aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc ttctccccat
    68521 gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc agtaatttct
    68581 acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac ttcgcctgtc
    68641 caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc atagattttt
    68701 ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt attggcactg
    68761 attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt taaacctgca
    68821 ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc tgtcatatca
    68881 gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt tcgccataaa
    68941 gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa tacccagccg
    69001 cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata ggtaggagat
    69061 tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc ccctgttttc
    69121 ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat atctccagtt
    69181 gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc tccagtgact
    69241 gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa ttggatcgct
    69301 tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt tcttaccctc
    69361 aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg aactgcgctg
    69421 gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac gatcaaagaa
    69481 aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc tccagccatt
    69541 ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc ttgcaagcga
    69601 aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac agtgactttc
    69661 gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat attgatcttt
    69721 gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt accaggtagc
    69781 gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc aagagagacc
    69841 tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt ctcatctcct
    69901 gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact accattgatc
    69961 acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt cagtccttcc
    70021 ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt tgcacgagga
    70081 gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt aaattttatt
    70141 tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat gataatggca
    70201 tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt aaaattctgg
    70261 aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa attgtgagcg
    70321 aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc taaagtcaaa
    70381 ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc cagttcttct
    70441 gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc cgcttcactg
    70501 ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc ttcttgcctt
    70561 ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga ttcttctgaa
    70621 aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt tttctctaca
    70681 tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga cgcaatcagc
    70741 ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa ttttatttta
    70801 tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt tatggaaaaa
    70861 tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac aaaagtcttg
    70921 tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt cacaacctcc
    70981 attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag gtaatttatc
    71041 cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg acacaactaa
    71101 ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt ttaataggtc
    71161 tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca aatggagctt
    71221 accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg agttagttag
    71281 tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt ctacgtgaaa
    71341 cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag ctagttttcc
    71401 aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag agccaattgc
    71461 gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct tgactgattc
    71521 aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg ctcgagccaa
    71581 gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt gttcagcgat
    71641 tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac tatgataccc
    71701 ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg tatgcaagac
    71761 gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa attcctttgc
    71821 ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca ctttctttcc
    71881 cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg ctgtgatgaa
    71941 gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat actatgaaac
    72001 tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat tgtataccta
    72061 ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga atgtaatagc
    72121 gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca aatgcataac
    72181 tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat gcgacaatcc
    72241 ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat tttatataca
    72301 aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg tatacgtgaa
    72361 taacttctag aaataaaaga aatttgcaaa accactccat ttatctactt ctacgtgcat
    72421 tttt
//
LOCUS       DANJJW010000005        62231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000005 DANJJW010000000
VERSION     DANJJW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            598..1287
                     /locus_tag="PWS82_000307"
     CDS             598..1287
                     /locus_tag="PWS82_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0905465.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="PWS82_000308"
     CDS             1274..2482
                     /locus_tag="PWS82_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0905466.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="PWS82_000309"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="PWS82_000309"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL0905467.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PWS82_000310"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PWS82_000310"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL0905468.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="PWS82_000311"
     CDS             complement(6585..7280)
                     /locus_tag="PWS82_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL0905469.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PWS82_000312"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PWS82_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL0905470.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="PWS82_000313"
     CDS             complement(8479..8670)
                     /locus_tag="PWS82_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL0905471.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="PWS82_000314"
     CDS             complement(8691..9581)
                     /locus_tag="PWS82_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL0905472.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="PWS82_000315"
     CDS             complement(9568..10335)
                     /locus_tag="PWS82_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0905473.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PWS82_000316"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PWS82_000316"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL0905474.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PWS82_000317"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PWS82_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL0905475.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PWS82_000318"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PWS82_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL0905476.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="PWS82_000319"
     CDS             14906..15289
                     /locus_tag="PWS82_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905477.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="PWS82_000320"
     CDS             complement(15457..16215)
                     /locus_tag="PWS82_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL0905478.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="PWS82_000321"
     CDS             complement(16233..17051)
                     /locus_tag="PWS82_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0905479.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PWS82_000322"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PWS82_000322"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL0905480.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PWS82_000323"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PWS82_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL0905481.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="PWS82_000324"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="PWS82_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL0905482.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="PWS82_000325"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="PWS82_000325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL0905483.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="PWS82_000326"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="PWS82_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL0905484.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="PWS82_000327"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="PWS82_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL0905485.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="PWS82_000328"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="PWS82_000328"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL0905486.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="PWS82_000329"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="PWS82_000329"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL0905487.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="PWS82_000330"
     CDS             27093..27392
                     /locus_tag="PWS82_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL0905488.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="PWS82_000331"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="PWS82_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0905489.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="PWS82_000332"
     CDS             27980..28216
                     /locus_tag="PWS82_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL0905490.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30233
                     /locus_tag="PWS82_000333"
     CDS             28359..30233
                     /locus_tag="PWS82_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL0905491.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDGLIG
                     ISARSTGTINVQIIMESLGGGGHFTNAATQLENTTVEETKQKLFAAIKENMNQIYDKE
                     "
     gene            30239..30691
                     /gene="rplI"
                     /locus_tag="PWS82_000334"
     CDS             30239..30691
                     /gene="rplI"
                     /locus_tag="PWS82_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL0905492.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30965..32332
                     /gene="dnaB"
                     /locus_tag="PWS82_000335"
     CDS             30965..32332
                     /gene="dnaB"
                     /locus_tag="PWS82_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL0905493.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33112..34404
                     /locus_tag="PWS82_000336"
     CDS             33112..34404
                     /locus_tag="PWS82_000336"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL0905494.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35140..36003
                     /locus_tag="PWS82_000337"
     CDS             35140..36003
                     /locus_tag="PWS82_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0905495.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36132..37754)
                     /locus_tag="PWS82_000338"
     CDS             complement(36132..37754)
                     /locus_tag="PWS82_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905496.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38003..38524
                     /locus_tag="PWS82_000339"
     CDS             38003..38524
                     /locus_tag="PWS82_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0905497.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38710..40440)
                     /gene="spxB"
                     /locus_tag="PWS82_000340"
     CDS             complement(38710..40440)
                     /gene="spxB"
                     /locus_tag="PWS82_000340"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL0905498.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40605..41423
                     /locus_tag="PWS82_000341"
     CDS             40605..41423
                     /locus_tag="PWS82_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL0905499.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41526..41723)
                     /locus_tag="PWS82_000342"
     CDS             complement(41526..41723)
                     /locus_tag="PWS82_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905500.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41892..42470
                     /locus_tag="PWS82_000343"
     CDS             41892..42470
                     /locus_tag="PWS82_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0905501.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42565..42702
                     /locus_tag="PWS82_000344"
     CDS             42565..42702
                     /locus_tag="PWS82_000344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905502.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43515..44018)
                     /locus_tag="PWS82_000345"
     CDS             complement(43515..44018)
                     /locus_tag="PWS82_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0905503.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44377..45402
                     /locus_tag="PWS82_000346"
     CDS             44377..45402
                     /locus_tag="PWS82_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0905504.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45725..46603
                     /locus_tag="PWS82_000347"
     CDS             45725..46603
                     /locus_tag="PWS82_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL0905505.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47079..47195
                     /locus_tag="PWS82_000348"
     CDS             47079..47195
                     /locus_tag="PWS82_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0905506.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48123..48461
                     /locus_tag="PWS82_000349"
     CDS             48123..48461
                     /locus_tag="PWS82_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905507.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48555..48785
                     /locus_tag="PWS82_000350"
     CDS             48555..48785
                     /locus_tag="PWS82_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905508.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49185..49556
                     /locus_tag="PWS82_000351"
     CDS             49185..49556
                     /locus_tag="PWS82_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL0905509.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49830..50522)
                     /locus_tag="PWS82_000352"
     CDS             complement(49830..50522)
                     /locus_tag="PWS82_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0905510.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50525..51310)
                     /locus_tag="PWS82_000353"
     CDS             complement(50525..51310)
                     /locus_tag="PWS82_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0905511.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51547..52947
                     /gene="lacG"
                     /locus_tag="PWS82_000354"
     CDS             51547..52947
                     /gene="lacG"
                     /locus_tag="PWS82_000354"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0905512.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            52972..53289
                     /locus_tag="PWS82_000355"
     CDS             52972..53289
                     /locus_tag="PWS82_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0905513.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53302..54975
                     /locus_tag="PWS82_000356"
     CDS             53302..54975
                     /locus_tag="PWS82_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL0905514.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            54989..56620
                     /locus_tag="PWS82_000357"
     CDS             54989..56620
                     /locus_tag="PWS82_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL0905515.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57492..58682
                     /locus_tag="PWS82_000358"
     CDS             57492..58682
                     /locus_tag="PWS82_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL0905516.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58816..61005
                     /locus_tag="PWS82_000359"
     CDS             58816..61005
                     /locus_tag="PWS82_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0905517.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61002..>62231
                     /locus_tag="PWS82_000360"
     CDS             61002..>62231
                     /locus_tag="PWS82_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0905518.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT        19388 a        11307 c        12290 g        19246 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga
    25441 aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa
    25501 agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc
    26881 tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacggcttg attgggatca
    30061 gtgccagaag taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag
    30121 gacacttcac aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga
    30181 agctatttgc tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat
    30241 gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt
    30301 tccagcaggt tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa
    30361 aagcagtatc agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat
    30421 cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt
    30481 gaaggcgaaa gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc
    30541 tgaagggtta aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa
    30601 tccgatccgt tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc
    30661 tacgatcaaa gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta
    30721 agtataacca gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat
    30781 gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa
    30841 aatagcataa agaaaacgag tataataaaa gagaactatt tttttattac actttcggta
    30901 cgaatgatga cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat
    30961 acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc
    31021 ggttttaggc tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga
    31081 gccaaaagac ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa
    31141 tgatcgtaat gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt
    31201 gttagaagat gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc
    31261 agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat
    31321 ccagacagca acaggaatcg ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat
    31381 tttagatgat gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt
    31441 tttatctatt gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa
    31501 caatgaagaa atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg
    31561 tttccagccc gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt
    31621 tgcattgaat attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag
    31681 tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga
    31741 ggccagccat ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc
    31801 gatggggagt ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc
    31861 tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt
    31921 gatcgattat ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc
    31981 agatatctct cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct
    32041 ttcacagtta tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat
    32101 tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga
    32161 ttattatgaa agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt
    32221 catcgaagtc atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt
    32281 tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg
    32341 ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga
    32401 acgactagag tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac
    32461 agtccctttt tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa
    32521 cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg
    32581 atcttacgac ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
    32641 aatatcatca aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac
    32701 ggcaggctca caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc
    32761 ccaatcaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca
    32821 ggacagcttt ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata
    32881 agtcgaaatt tcacaaaaat ttgagaagca attttcgtac attccttctt atttcttgaa
    32941 gctgaccact tctgttacaa cctctttttt aataaccatt ctaaaaagat aaagttcgtt
    33001 ttttaagcgt ttttataaaa taaaaataaa aatcattcga ttttatttga aatatgtatc
    33061 tattattgat acaatggttc aggattaaat aatatagaac gaggtgttca catgtcatct
    33121 gtagtagtag taggtacgca atggggcgat gaagggaaag gaaaaatcac agactttctc
    33181 agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg acaatgcagg gcacacaatt
    33241 aagtttaacg gtgtgactta taaactgcat ttgattcctt ctgggatttt ttataaagac
    33301 aagatcagtg tgatcggaaa cggcgttgta gtcaatccta aatcattggt taaagaatta
    33361 gcttatttac atgaaaaagg tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac
    33421 gtaatcttgc cttatcatat caaattggac caattacaag aagatgctaa gggcgaaaat
    33481 aagattggta caacgattaa aggaatcgga cctgcctata tggataaagc agctcgtgta
    33541 ggtatccgta ttgcagactt attagataaa gaaatttttg aagaacgtct tcgtttgaat
    33601 ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt cagaaccact tgattttgac
    33661 gatatttttg aagaatatta tgaatacgga caacaaatca aacaatacgt cacagatact
    33721 tccgttatct tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa
    33781 ggtgtaatgc tggatatcga ccagggaact tatccttttg ttacttcttc taacccagta
    33841 gccggtgggg taacaatcgg aagcggtgta gggccttcaa aaatcaataa agttgtcggc
    33901 gtctgcaaag cctatacgtc acgtgtcggt gatggaccat ttccaactga attatttgat
    33961 gaaacaggag aacaaatccg cgaagtagga cgagaatacg gtacgacaac tggtcgtccg
    34021 cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt
    34081 accaacctat ctttaaactc gatcgatgta ttaagtggat tggaaacagt aaaaatttgt
    34141 acggcttatg aattagatgg cgaactaatc taccactatc cagcaagctt gaaagagttg
    34201 aaccgctgca aaccaatcta tgaagaattg ccaggctggt cagaagacat tacaggatgc
    34261 aaaacacttg cagaactgcc agaaaacgca cgtaactatg tacgtcgtat ctcagagctt
    34321 gttggtgtac gtatctcaac attttctgtc ggacctgata gaacacaaac aaacatctta
    34381 gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac tgaatcagga cgttcttacc
    34441 agagcgagtc gaagtgttta tctatacgat aataataact tgaacactgt ttattcagaa
    34501 taagggactg tgacaaaaac cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa
    34561 gcttgcatct acggcactaa gcccaatcag atgaaaaata tggagtgcta tttttatctg
    34621 attgttctta ctgcttgatg cagagcagct ttttcacaac ctctccacta aacggtgttc
    34681 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg
    34741 aaattgttcc ttgtgactcg gagctaaacg gcaggctcac aacctcttgg taaacggtgt
    34801 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttt
    34861 ccgttttttt ctctaatact caggtcaaaa cgctgatttc acaacctctg attcacggtg
    34921 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt
    34981 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc ttattttgat
    35041 tagaacttgc gaaaataaac cggttaaagt gtgtctagcg caagagattt gctataatag
    35101 acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat
    35161 tcttgttgtg atctgccata ccatgtatta gcaaaagaaa aagttgattt tttatcaatg
    35221 aatctccaat tgaatgatca agagttagta gatgacctcg gaaagacatt caattatgac
    35281 cgctacatga atcagctgaa aaatggtgcg atgcctacga catcacaaat caatgttggc
    35341 cgatatttag aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct
    35401 ttttccagtg gattaagcgg ctcttacagt agtgcgcttc aagcggttga attgctgaag
    35461 gaagaatatg aagaccctga gatctatgtt tttgatacaa aagctgcaag tattggggaa
    35521 gggcttttag tattggaagc tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc
    35581 ctagagtggc tggaaagtaa ttatttgaaa gtccattcat gggtaacagt agacgatctg
    35641 aaatatctag aacgaggcgg taggatttcc aaagcaagtg ctactcttgg cagtctattg
    35701 agtgtaaagc ctattattac ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt
    35761 cgaggaagaa acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca
    35821 ccacttgatc aaatcgttta tgtggcttac gctggggatc tagagagcgc ggagaaggca
    35881 aaacggcttt tggaagagca gataaagatg aaagatgtga agctttatcc cttaggacca
    35941 acgatcgcca gtcacacagg ctatggctgt atcgcgattt tttcgatggg aatcagtcga
    36001 taaaagcagt aactcattct gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac
    36061 tcttaaaaga tagatgagca tcctatctct tagaggtcgc tgttttttta ttttttaagc
    36121 taaataggcc attatgccca taactcgttc aattgttttt gaatatctgc attttccaaa
    36181 aattcatcat atgtcgtatc agctcgatcg atcacacctt tatccgaaac ggcaatgatg
    36241 cggtttgcca atgtttggat aaattgatgg tcatgtgacg caaataagat tgagccagta
    36301 aatgccatca agccgtcatt caatgctgtg attgattcta agtctaaatg gttcgttgga
    36361 tcatctaata caagtacgtt agctttcgaa agcatcattt ttgaaagcat gcaacggact
    36421 ttctctccac cagataaaac attcactggt ttcaatactt cttcaccaga aaacaacatt
    36481 cttcctaaga agcttcttaa gaaggtgtta tcatcttctt ctttacttgc atattgacgc
    36541 aaccagtcta aaatcgttag atcagtatcg aattctttcg ttgtatcctt tggtaaataa
    36601 gaattacttg ttgtcacacc ccaacgtact gttccagtat ctggcgtaat ttcacccatg
    36661 ataactttga atagcgttgt tgttgttata tcattttctg cgacgaaagc gaccttgtct
    36721 tctttgctca aattaaaaga tatatcatct aaaattttct ttccatcaat cgttacactt
    36781 acattttcta cctgcaataa gtcgtttcca atctcacgtt caggtttgaa tccaacgaat
    36841 ggataacgtc tagaagaagg ttggatatcg tctaaagtaa ttttttcaag cattttttta
    36901 cgtgaagttg cttgttttga ttttgatgca ttcgcactaa agcgggcaat aaattcttgg
    36961 agttctttga tttgttcttc ttttttggca tttgcgtttg cttgaagttt agccgctagt
    37021 tgactagatt ccaaccaaaa atcataattt cctacataaa gtttgatttt accaaaatct
    37081 agatccgcca tatgtgtaca tactttgttc aaaaagtgac ggtcatggga tacaacgatc
    37141 acagtatttt caaaattgat caaaaattct tccagccaat tgatcgattg gatatccaga
    37201 ccgtttgttg gttcgtctag caaaagaaca tcaggtttac caaatagtgt ttgtgctaat
    37261 aacactttta ccttttgtcc accagttaat tcactcattt gctgatggtg caattcttca
    37321 gggatgttca gaccttgcag taaaacagct gcttctggtt cagcttccca tccattaagc
    37381 tcagcaaatt ctccttctaa ttcagctgct cgaataccat cttcgtcagt gaaatcctct
    37441 ttcatgtaaa tcgcatcttt ttctttcagc acttcatata gacgcttgtg tcccatgatg
    37501 actgtttcaa gtacagtata gtcttcatag tcgtaatggt tctgtttcaa tacggccatc
    37561 cgttcatctg gtcccatagt aacggaacct gtggaaggct gcagatcccc tgataacact
    37621 ttaaggaacg ttgatttacc tgcaccattt gcaccaatca agccatagca gtttccgggg
    37681 gtaaatttaa tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa actcacatca
    37741 tttacagtaa tcaatttttt tcctcacttt tatcattatt agttttgaca agtcttatag
    37801 aattatatag gggaaaggta aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg
    37861 aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa tagctactat aggatttatt
    37921 agtttgcaac agcataattt ttgacgttat ttataagagg gagtactata aaactgtaaa
    37981 agaaatgaaa gaaggattga aaatggatga aaaaagaacc atcgactggg gctcattgat
    38041 tttgggaatt ttatttgtac ttgtctcatt gttatcattt caagaccctg tcggaaattt
    38101 agttgcaatt gtagtagtct ttgcgatttt tgcctttatt aaagggatat ttgaactgtt
    38161 tgtacggaac agattaaaag agctaacagg atataaagga aagatgccct taataatcgg
    38221 gatcatcgat attctagttg gcgtcttttt cttattcaat attggtgcag gagtcgcagc
    38281 attgcctttt gtttttgcag tatggtttat tgctgattca gttttagcgt tatttactgc
    38341 tgacttggca agaggagtaa gtgaaggata ttattggttt acgatcatcg tcaatatttt
    38401 aggaatttta ttaggaattt tcttattgtt taatccaatt tcttctgcgt tgacattaag
    38461 ctttttagtt ggattctatt ttatgttatt tggtatcaca catatcgtct atgcatttag
    38521 ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt acagttttac tgttgtaatt
    38581 ggaagccggc ttttttatca tggacaaata tgcacgaggt gatttttagg tgtcgagaca
    38641 aatttatgca aaaatgtgag aagctatata cacaaatttt atcttaatac tcgaagcata
    38701 gcgcagttgt cacaacctct tttcatataa ttcattcaat actggcaaat catggacttc
    38761 atatttttct ttgaatgctt ggatttcttc ttcagaatat tttcttggat ctaaatgcag
    38821 atcttctact ggcaatgggc gcttgttatt gatcttcaca tcgattacta caggacgatc
    38881 tgattttgca gctgcatcaa aggcaaatcg aagttgcgag tattctgtga ctgtaaagct
    38941 atttgccccg agcgcttctc caactttacc gaaatctgca tcttctaaaa atacaccaaa
    39001 tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa
    39061 gacaacatta ataattggaa gctgatattt gacttgtgta atgatatctt gcatgaccat
    39121 agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc
    39181 ggcaattcct cctggcacac cattacccat agttgcgaac catccagatg tcgtatgttt
    39241 ttgtttaccg ttcatattca ataaacgaat agaatggata gtcgtgttac cgacatctgt
    39301 gacgaaaatc gcatcgtctt cagcaatacg attgatttct ttatagacag cctctggacg
    39361 aatcggttct tcctctcgat cttcaaaaga tttcaaccaa tttacccatt tttctttatt
    39421 ttcttgattt gctaggtacc agctatcagc agggcgagca tcccctaact cgacaagtct
    39481 ttgcaaagct gtgtttgcat caccgaggat cgacaaagat gtttcatggc gtcgtccaaa
    39541 ttttgacgca tcgatatcga tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc
    39601 aaatgggaag tcgctaccga caaataagat cagatcggct tctgccaatg cttcatttcc
    39661 gggtttagta gcgacacgtc cagcaaaacc aagaaagttt tcataatcat caggaataat
    39721 tccttttgct agaacggaag cgacaacagg aactgaaaaa tgttctgaga attctttgat
    39781 ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga actggttttt ttgctttttc
    39841 aagataaggc aaagcagcta gcaaatcttt ttcttctggt aatatcacac ttgttttgtg
    39901 agtgtgagcg gtggagaaag gttgttcgtc aatttcttca aaaccaagat caacagggat
    39961 agtcacgata gccacgcctt tatgctcata ggcggcttta atggcttcat ctactacatg
    40021 cggaagactt tgtggtgtca tgactgtacg attgtatacg ctaacatctg cgaaaatagg
    40081 attttcattt agttcctgga aagagttata gttcatggat gtagaagcaa cttgtcctaa
    40141 gaacgctact agaggcacat ggtccatctg tgcatcatac aaaccattga tcaaatgtgt
    40201 cgcaccaggt cctgcagagc cgaagacagc gccgactttc cccgttaatt ttgcatctgc
    40261 agcggctgcg agtgcgccga cttcttcatg acgaacttga atgtagttga tttcggtttt
    40321 ttcttgatat agcgcatcca taattgaatt gaaagatcct ccgggaatgc cgtaaatatg
    40381 gtcgataccc cagctttcaa gaacttttac cattgcgatt cctgcattga tttttgacat
    40441 ggtgtttcac tcctttagca atgatgatac ataaattttt tgtatctaga gtatagaact
    40501 ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa
    40561 tatgctagag tagtacatag atagattcaa gagaggggaa tggcatggtt gaatggctat
    40621 tgagacttga accttggcag caagcacttg ctgggacagc ttttacttat tttatgacag
    40681 ctcttggtgc cgggttggtt tttttcttta aagaaatcaa aaaagaagtg ctaaacttga
    40741 tgttaggatt tgcttctggt gtcatgattg ctgcaagttt ttggtctttg ttagatccgg
    40801 ctattacaaa agcagaagaa aatggagata ttgcttggct agttgtaagt attgggtttg
    40861 gtttaggcgg tgtgttttta tatatggcag ataaaacact gccccatatg cattttggac
    40921 cacaacatga agcagaagga ttgcctaccc atttaaaacg gacgatcttg cttgtttttt
    40981 ctattacatt gcataacatt cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag
    41041 caactgcgga taatccgaca gccgctatat tagccgctgt ttcggtggcg ctaggtattg
    41101 gtatccaaaa cttcccagaa ggagcagctg tatcaatccc gttgcgacaa gaaggtctga
    41161 gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat cgttgaacca attgctggag
    41221 tgattggagc gctactagta acgaaagtgg agctgctgct tccttatgca cttgcttttg
    41281 ccgcaggagc aatgatttat gtcgtggtgg aagaattgat ccctgaagca cagcagacat
    41341 tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag tggatttatc attatgatga
    41401 ttttggatgt ggcattagga taaataaaaa aaatatatat gatatatcaa atagggccgc
    41461 gaacttatat cgcgacccta tttttttagt aagttatatc ataatgaagt gaatggtatc
    41521 atacattaat aatagttttt tctatgtgta tttcctttga tccagcgaac aagccagatg
    41581 ataagtgcga ttgggattag aactttgacc gcaaaccata aaagcccgct taggacactg
    41641 acaaaaagac tgatgacgaa gctagctgca attagcacta aaagaaccat gaagatattt
    41701 aagaacatat ttttttgacg catacaaaac cctcctcttg ttcattcttt actatagaaa
    41761 aagtataaca gggattgttt aaaaacacct ccaactgaag actgattttt acttcatcta
    41821 cttaaatgat caaacaattg ctttttctct ggtttatcat ctaaaataga gcggaaaagg
    41881 aggagaatat catgcctaaa aatcgtcttg aagcatttac agatgctgta atcgcgatta
    41941 tcatgactat tttagtttta gaacttcatg aacctaacgg agacacgtgg gaggcgctcg
    42001 ctgcattagg ttaccgattt tttatctata ttgtcagttt tatcatttta gcgatttatt
    42061 ggaacaatca tcatcattta tttcaagttg tacataagat aaatggacgg gtcttatggg
    42121 cgaacagttt ctttgttttt gcgctttcgc tttttccgtt tgcaacttca tggatcagtg
    42181 agtacatcaa tagcctggct ccagaaatga cttatggttt tgttaccttg accgctaata
    42241 ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa taaaggccac gagaaagcat
    42301 tgaaggcgat gttgaataat tacaagaaat cgtatctatc gattggtttg aatgtgttag
    42361 gaattgtctt agggtattta gtagcgccag tagccgtgct gatcgcaaac atcgtgattc
    42421 tagctgcttg gatcatccca agtcgtacca tcgaatcaca atatcggtaa aatgatccgt
    42481 gtttaaaatt taagagaaaa atatagaaaa aataaataaa cgatcaaata tccgagtgat
    42541 actagtattc aaagagggat gcagatggat caaaaaagaa ttcatgtagg actgattact
    42601 agttttgtca cgatgcttat ttatttggat caaggtcatt tgacttggac agtagttggt
    42661 gtcgtaggat ttttgacatt attcacagca ataaaaaaat aaacatcttt atgtaatctg
    42721 atgttctatc tatttaagaa aagcatgttt acagcagtat gattctgttg taaacgtgtt
    42781 tttatttgtg gaaaatttaa tttttagaaa agtgtatcga aaaatagata gttagttttt
    42841 ccacagtgga taacttcgta agagataaaa ataaaaaatt agtcaaagaa gggcgtatag
    42901 atttcttttg ataggagata tacaacagaa atgaataaat attaacgtgt tttcaaacag
    42961 tataaaacgg tgtggttttt ggaataaaaa aataacatat ctcattttac gcttttgtta
    43021 ttttatactt aaaaaataac gacacagaaa ataccgtgtc tcagatttct ctactacttc
    43081 taaaaactac tgtttttttc ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata
    43141 ctggcagaat caaggagaag ggagcctaaa taaggagaaa aaccatagaa aatataaagt
    43201 aattacttat aaaacaagct ttttatagaa aaataatata cctgagtagg gtataaatta
    43261 agacggaatt cctttctaga ttgagatatt gactaggaag aatgttaaga ttttaagcct
    43321 ttaagctaaa aaaaaaccct ttttgttaaa taaggggaat atagcctgtc agtttcaaac
    43381 tgcaagtcgt cctgctgcaa taatatagta gattagagat gaaaatagat ttttttaaac
    43441 caatgactga ataaaaagac cgtcatagat acacattgga atgtgtctat tacggtcttt
    43501 ttttacgaac gattttattg aagacggaca gttcgggtca aaaataaaaa agctacgatc
    43561 agaaataaga ataaaatgta tcccaaaata atggctagac ggtctaactt atttgtcgat
    43621 agatgtttgt ttagaagttg ataggcccat aaacctatca gtcctcctgt tgtattagtt
    43681 atcaaatcag tgatgtccgc tacgccaata ccgaatagga attgaatcaa ttctgccaag
    43741 aagcttacaa ggaaaataat ccctaatttt ttagaaaaag aaagtctctt gaaattgaca
    43801 ttcaaaagca ccccaaaagg aaaaaagaca atcaagttat aagtggtctc acgtaatacc
    43861 gtcgtatttt caaaagaaaa agtagaaaaa gggacgagat tgacattgct gtaagaataa
    43921 gtgagaaatt gtggaagatt aaatgatagc ttgaataaaa tcaaccataa tagaaagaat
    43981 aaataaatcc ctaataaaaa ttttgataca taccgcatag acatctcctc catttaaaaa
    44041 gtataccatc gaaaaccgct gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta
    44101 gttatcgtac gaaaattaga agcttttttt tgttgggaat ccggtttctt taacaaagct
    44161 aagatagcga gttgcttgga aaagttgcta tcttttttct gtcaaaacga ctcggaatgt
    44221 tcgttttttg ttctaatttc tccaagttgt ttactttttg atactaagtt tatgaaaaat
    44281 caagaaatag ttgttatact aggtaatgct gacaaacggg attggcattt catcttggtt
    44341 cagctttttt tccaattact aggaaggagg gatagattgt tcaagactta ttttcgtttc
    44401 aataaaatac aagataatcc ttttcgtatc atgaatctga tgctacttat ctttattgta
    44461 ttatggttag gttacataaa tcatgatttt ttgatttcaa gtttttcatc attagggatc
    44521 tttacttttt tctattatca aaacataccg atgaaatatt tgctgaaacg tatgtttctg
    44581 atcggttgcg gactgttggt tgctttcgtt ttggggatct tatcgactta tgttttatgg
    44641 cttgaaccat ttgccgtagc tttggttgct ttttccagca gattcgtgtt gcgattattc
    44701 catatttcta aaccaggtgg cttgtttttt gcgatgcttt ctgccatggg aacgagcatg
    44761 cagctgccaa tcgctcaatt accgatcgtc tcactttatt tttttatggg tgtcgttttt
    44821 gccctgattg cggcagtgat tacaaaatta ctggattcac ggcctgagca gacaattgaa
    44881 aaagcaactt taaaagaaag atttcacgaa gagcctttag tgatcattga ttctgttttc
    44941 tacagcgctg ccttatttct ttccgtttat gtcagtcacg gcttgaatct gcataatcct
    45001 tattggttga ccttgtcatg tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag
    45061 catcgacaag tccaatatct gattggatcg atgggtgggt tatgtgtgtc ggcattcttg
    45121 tcatttgtgc cgtttacgca gttgcagacc atttttctga tcacttttct atatggaatc
    45181 gcacaatttc tagttgctag aaattatgca gttgctaata tttttcttaa tccgatggcg
    45241 ttgatgctat ctacacttat tcgtggagca tacttgattt ctttgatcga atatcgtttt
    45301 ttagggatcg tcataggaag tttcatcggt ttaggtgttg cgtgggtaat gacggttggt
    45361 ttgcaacatt atttaacggt tgttcgtcaa aaattagaat agaaggacaa atatatgtgc
    45421 caaaagaggt tatgacaaga tttttgtcac aacctctaat tcaaggtgtt caaaagccag
    45481 attctcagca ttaagtcaaa ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt
    45541 atatttattc tatcaaaaaa ttttgtttat gatggtaact gaataaactt tcctgagatg
    45601 gtcaatgctt tccttgaaag tcttacagat attctttctt catagaaagt ttaagtttta
    45661 tagttagtgt acaataaaag tatgtttata ccatatggtg tccggataat cagtcattta
    45721 aacaatgagg ttcaaagata tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat
    45781 ccaaattctg gcagaaactt taactcttcc caaagtcgtt atagagcaaa tgattgtaca
    45841 agcaaaaaac atccttgatg aggtgtttga gcaatatagt catgaactaa tggatcctaa
    45901 taaagcaaag gcgactgcaa agaaaataga caaagtctat gaagaggaac cttttgcagc
    45961 attagctatt tatctatatg cagcttctca aagttggaaa aaaatttatc aaccattgaa
    46021 gattccaaga actatctatt tggcaacaat gaatgctttt actagattta ttcatgaaca
    46081 tttccaagta acaggaagct atcgctttga ccgtggtttt tggatttggc attacactag
    46141 tggcttgatc tttcggattg gtgaattgga gtacgaaatg acatattttc cgaaagggta
    46201 cacggatcta agattagaag gaaagcagtg cctttctctt catattcctt ccgatgcaga
    46261 tttatctcag aataagataa gagatagtta cgaaagtgcc attcgttttt ttgaacttta
    46321 ttttcctgat taccattacc aagtgatgta cacagatact tggttactat cacccaatct
    46381 tactaaatgg ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt atcgattatt
    46441 atcagttgat gaacaggacg atagtggagt cccttggatc tttggacgag tagatgccca
    46501 aatacatgat tatccagaaa gtacgagttt acagcgacaa gcaaaagagc aattgcttgc
    46561 aggcgagcac attggctctg gcttaggtat tcttacttca taagtttagt acttcgaaat
    46621 agtttaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    46681 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    46741 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    46801 tttttagtgt tctatcgaac acacagtacg ttcaataggt aaactagcta taacaataga
    46861 tattaaagtt aacggacaag agggcctttt ttaggtagct aaaacgtaat aatgaaaatt
    46921 tatttccaga gataggagtc aatcagctag tctgatcgac tgttttaaca agttgacttt
    46981 cattttaact tttgatacca tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt
    47041 agcatgacta caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa
    47101 gtgcataccg ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca
    47161 tcaaagatgt gaaacggaat agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa
    47221 tagccgaaaa acgtttttcg gctattttca ataatcggtt tttgtgtaag gtttccgaaa
    47281 gaactgctgt tgattttcca tttttaacat atgataaatt ggtagaatct ttcttttcaa
    47341 attgatacca ttccctaatt ccagttatat agttaactac agaaccacaa agcacgtacg
    47401 tttataaaag tattatctgt agttattttt caattccctt tcaacctctt attttgacca
    47461 gtagtattga tcactttacc ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa
    47521 gaatggaaac gtaactacat aatcaaaggt tttgcgaaat atttcatgaa atatttcatg
    47581 tagaagcgag caatacggtt gcaaataacc attatcgttt aataagcagt tacgaaagct
    47641 cccaatatct ctaacgttca gctcactcca tttcttttct tcaaaaaaat cagtgattcg
    47701 tatctctttc tatttgtcga aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa
    47761 aaaaataaaa aagcagttac tattgctaga ataacatcaa ggaataataa gttttggttt
    47821 tcagataata gtcgataagt tgtgtattgg aattttagat acagtaataa agtatttttc
    47881 atgaaatttt gtgagaatca ctattacaag tatctttaat aaaattaatg gttaaggagg
    47941 aacttaaagt agaatgtaaa tgaatagttg tagtcagtcg attgtgatac tagactcaaa
    48001 gttgcaggaa taagtttttc aatttgtgat tggaagataa aaaatgaaaa agaagggctt
    48061 ttttttatac tttgttgatt gtagtgttta ttttccctct gtttagccaa gtaacatttt
    48121 ctatgactca accagtttca agtgaggaac atgataacct ttttcgtgct acagaagagt
    48181 ttaagaaata tcaggaaaat attgttccgg agactccatt agctagtgag ccaattactg
    48241 atattgatgg aaaccaaata ggatggtata tacagtacga agttatggtg gtaaataatg
    48301 actcaatagt aaaaaataca gtaggcttag agctaacacc gattgaaaag gtgagttcat
    48361 tgttaacgta tatttatact gatgaaagtg acgagactga gatcatttta acggattact
    48421 caaaaattgc tactgataaa aagtatatat tgtggatgta gacactagtg aaatacttta
    48481 tgatatagaa attgatgaaa atttactaga tgtagcacaa gaaatcgatc atcaaaagca
    48541 ggaagctatt cgatatgctg gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag
    48601 caaaagttag agaagatgaa aatgagtgga aagaattcaa gagtcgctac tcaattaact
    48661 cgactccgac atttacagtt tacagagaag gatcgataga aaaaactgtt ttttggacta
    48721 aagaaagtgg aatgtcttta gctgaagtag aagagttttt ggattatgtt agtatgcaac
    48781 agtaagggag ttcttattat gaaaaatttt gacacatatt atgttattgt tttttcgata
    48841 tctttgctgt taatattact aactttaatg cagtataaag agtatccaga gtattttatt
    48901 ttaacttttg tatatttagt aagtagctac acacttctat ataaattcag aagttcttct
    48961 agagtttcta aagtttgttt cttaattagc ttattgattc tataatattt ttgatttata
    49021 taggaaatta aaaatattat agaatcaaca ctcagagaac gggttggttt ttaatagatt
    49081 taataagtac tgataatgaa atttctcctg gttgggaaaa cgaagttggt taccctgtag
    49141 aagaattttt tcaagattac tttgcggagt atgccgatag gataatgaga gattacgtaa
    49201 cagataaaac tcaagaagtc atcaatcaat cccagtcatt aaatgaatta tctgataagg
    49261 cgaaagaaga agcagttgcc attatttctt cagggattag tgactatatt gatatgagtg
    49321 gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa
    49381 cttttgatgt gaaggcagat cttaagttta atgatactta cctagtagga aaattaatta
    49441 atatccttgg gacagagaaa gatacggaaa cgtttatttc ctctttgtct atgttgttta
    49501 cagataagca tttttcaact cagcttggtg gagaggaact agtggaaatc ttttaaccat
    49561 tacgcccagc attaataaaa gtatatcaaa aaagtactcc tgatgatttt taagattggt
    49621 ttgagaaata atgtgaaaac actattttta atgttaatta tagatagaga tgaacgcgtc
    49681 atatggttaa aagaatgaaa aagtcaaaga tacactgcta tatttcacta aggatatagc
    49741 agtgtatctt tcatgtcgtt tcactggcag ttttttataa aaggaggtgt gcatttcagt
    49801 tgcttttctt tggtagttag ttgaatcgtt taagaattaa cggatgtggc agacaggtgc
    49861 tctgtccaaa aaccagtaaa atctttgatg gttccatctt tatttagctg tgcctgttca
    49921 aatactgttt tgtttttacc gaatggatca attgcaataa ttgttccgat ccccgcacgt
    49981 ttggcagcaa gtaatcctga gtaagcatct tcgataacaa gacaatcagc cggagctaag
    50041 ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg gtttcccagg gaaagagccg
    50101 tcgtcataga caatcttgtc ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa
    50161 aaagcaacat tctctttgac agtagccgtg gcaatcgtta atggaatctg ttggtcagtc
    50221 agctggtcaa gagtcgcttc aagccctttt gtcaactgaa gctcgttttc attttgcaag
    50281 caaagcatac gataatagct ttctttttct tctgacaata cggcgatctc agaggcggtc
    50341 aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg tccgaccatg aatatttttt
    50401 aaaatatcct cgtctgataa accatctttt gtatgatcat gtatcatatg cagccaagca
    50461 gcttcatgca gatgagagtc caaaaacatt gttccgttga aatcaaatat aatccctttc
    50521 atagttacgc tcctatagtg gtattttcgg ttgaatcagc aatttgcaga tcgacatatt
    50581 gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat
    50641 catgcaaatt ataaaacaca taaaaatcat ctcgattgaa ttttttatta tcaacgagta
    50701 gatatttctt acgggaatta tttagagcga tctgctgtgc ttctccttct tcggtactgt
    50761 aagtagagat cgcttcgttg tgaacgccat ttgaactgat aaatgctttt gtaaacttta
    50821 gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc gccggtattt ttccggaaat
    50881 caccgccaat cagtagaatt tctggtccat ctgcttgagc aagaatatca aaaactggat
    50941 aattatttgt aatgatccga attttcttgt tcagtaaatg cgtcgccaat agttcgattg
    51001 ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat caaggaggct gctgtggcag
    51061 caatttcttt tttttcttga atctgcagag tggctttttc agtgtgagaa agttcttgat
    51121 ctaaagtgta ggaaagactt tgagcccctc catggacacg cagcagcttg cccgattttt
    51181 ctaattcatc taaatctctg cgaacggtca tatcagaaac atctaattga ttgatgattt
    51241 cattaacggt aaggattcct tttttattga ccatttctac gatagtaagc aatctttctt
    51301 ttttcaacat tgtcttcacc tctaatttaa ggataatcta tcatataaac ggacaacagt
    51361 caatctaatt cgaacaaaag taaacattta gggtggtttt tatgtttgtt acaatagatt
    51421 ttattgctgt tttctggttt ttatgattat ttgtgtttgt ttttgttgac aaatgttttg
    51481 gatgcgttta caatgcaatc agttagaaag agcgaaaact gattatagat ttgaggagag
    51541 atgtacatgc ttaaattacc agaagatttt atttttggcg gagccacagc agcctatcag
    51601 gttgaaggag ccactaagga aggtggcaaa ggcgctgttg cttgggatga ctttttagag
    51661 gaacaagggc gattcagtcc cgatccggca agtgattttt atcatcaata tgcaaaggac
    51721 attgaattgt gtgaacgttt tggtgtaaat ggtctaagat tatccattgc ttggagccgc
    51781 atttttcctg atggagctgg gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg
    51841 ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc
    51901 cctaaacgtt tgtttgacca tggagatttt ttgaatcgag aaacaataga agcatttgtt
    51961 tcctatgcca ttttttgttt tcatgaattc aaagaagtca aggtgtggag tacctttaat
    52021 gaaatctatc cagttgcaac caatcaatat ttgttaggtg ttttcccacc aggaatcaaa
    52081 tatgatttta caaagatcgt cgcctgtctt cataatatga tggttgctca tgcccgtgta
    52141 gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg gtgtagttca ctcattagag
    52201 acgaaatatg cggcaacaga cgcgccggaa gataagcatg ctgccttttt agacgatgca
    52261 ttgtcgatcc gtttcttatt agatgctacc tatttaggct attattcaac tgaaaccttg
    52321 actgctcttg atgagatatg tgaagcaaat caggccagct atcattttcc tgaagaagat
    52381 ttcgttgaat tgaaaaaagc atccacgcgt aatgactact tagggatcaa tcactaccaa
    52441 tgccattttg tcaaagctta cgatggagaa aatgcaatcc atcataacgg tacgggtgaa
    52501 aaagggacct ctgtttataa agtgaaggga atcggagaac gtatttataa agaaggaatt
    52561 cctcgaactg attgggactg gctgatttat ccagaaggtc tatatgactt gttgctgcga
    52621 attaaaagcg attacccaca ttacaacaaa atctatatca ctgagaacgg gatggggtat
    52681 aaagatcagt ttgaagatgg cattattatg gatcagccga gaatcgacta tttgagagtc
    52741 tatttggaat ccttaagcaa ggcgataaca gccggggtaa atgtgaaggg gtattttttg
    52801 tggtcgctta tggatctttt ctcatggaca aatgggtata acaaaaggta tggcttgttt
    52861 tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat cagcttactg gtacaagttg
    52921 gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg aggaagaaaa gatgaataaa
    52981 gaggaattac aaatgcttgg ttttgagatc gttgcatatt caggcgacgc tcgcagtaca
    53041 ttattgaaat tattgaaaga agtaagacaa ggaaactttg aacatgttga ttcggcgttg
    53101 aaagaagccg acgaaaattt aacccttgca cataattcgc aaacaaaaat tttagcggaa
    53161 gaagcatctg ggaaagagat ggagatggga ttcattttta tccatgggca ggatcattta
    53221 atgaccactt tattgctgag agatctgatt caagacttta ttgtactgta cagacagaat
    53281 caaggatagg agaagaacgc tatggatgga atcattaaac aaatcgaaaa agggaaacca
    53341 ttttttgaaa aaatctcacg taatagttat ttaggagccg ttcgtgacgg atttttaaca
    53401 gcgatgccag ctattttatt ttcaagcatt ttcattttga ttgctgcggt tcctgagatt
    53461 tttggttacc aatggcctga atctgtctcc acatggctat ggaaagtcta taattattcg
    53521 atgggtgttg tagggttact cgtttcagca accactgctc gctgcttggc tgattcgatg
    53581 aatcgtcgct taccgaaaaa caaagtaatc aacaccacct ctgtgatgct ggcctctgtg
    53641 gtaagtttcc tgttgttaag cgtctctccg attgaaggtg gattttcaac ggaatatatg
    53701 gggacaaagg gactactggc ttcctttgtc gctgcatttt taacagttaa tttatataag
    53761 ttttgtgtgc tgaaagatat taccattaaa atgcctaaag aagttcctgg aacgatttcg
    53821 caaaccttcc gagatatttt tcctttctct ttttcagtat ttgcggccgt tttaattgat
    53881 ttaggcattc gacaaatctt ccaaatctca tttgcagaag caatcattac ggtgctgcag
    53941 ccgctattta cagcagctga cgggtactta ggaatcgcta ttatctgggg agcgatggcg
    54001 ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt
    54061 atctatgcca atgtggaaac caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa
    54121 gtactgacgg tgggtttagg gaattttgta ggaactatgg gaggaactgg ggcaacattg
    54181 gttgttcctt ttatcttcct actctttgct aagtctaagc agttgaaggc agttgggaaa
    54241 gcttccttta ttccagtaag tttcgctgtc aacgaaccat tgctatttgc ggcacccatg
    54301 attttgaacc cttatttttt tattccattt ttgttaacac ctgttgtaaa cgtttgcctg
    54361 ttcaagtttt ttgtcgatgt attgggcatg aacagcttta tgtacgtttt gccttgggca
    54421 actccagcac cgatcggttt ggttctagga acgggcatgg gtatcttagc ttttgttcta
    54481 gcagcggttc taatcgtcgt tgacttcctg atctatctac cattttgcaa agcatacgat
    54541 gctgtcttag tagagaaaga agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct
    54601 tctgccgatg tacgtgagga agtacctgtg gcagcgagtc aagttatcag cgatgcaaat
    54661 aaagaaatca atgtgttggt actttgtgct ggcgcaggga caagtgcgat gttagctaat
    54721 gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat
    54781 ggttctcatt acgaaatgct gcaggatttt gatatggtgg tcttagcacc gcaagtcaat
    54841 tcttactatg atgatatcaa agaagacacc gatcgcttag gaatcaagct ggttgcgaca
    54901 aaaggagcag aatatatcgg attaacgaga gatcctgaaa aagctgttca gtttattctg
    54961 aaccaatttc aataaaggag gagccataat gaaaatggga atttggttgc gtaaagctgt
    55021 tttatttgca gctgtgttag gggctttggg ggctgtttca aaaactgttg aagcatctgc
    55081 cagcgaattt attcgcggag cagatatttc gatcctgtca gatatggaaa aatctggtgc
    55141 aaaatattat gaagacgata tttcaaaaga tgccttagag attttaaaaa ataatggtgt
    55201 caactatgtt cgactgcgtt tgtggcagga tccttataat agtaatggag aaagttatgg
    55261 agcagggaca aatgatctgg aaacgacgat tgaccttgca aaacgagcaa aaaatctggg
    55321 acttaaggtg ctgctggatt ttcactacag tgatttttgg gttgatcctg ggaaacagaa
    55381 tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg aatacggcat tgtacgacta
    55441 tacaaagaac gttctgtcgg aaatgaagca gctggacgtt tatcctgata tggttcagat
    55501 cggcaatgaa ctgaatagcg gaatgctctg gccttatggc aagtcttggg gagaaggcgg
    55561 gggagaattt gatcgattgg ctgctttttt gaagtctggt atccaagctg ttcgagacac
    55621 tcaacctaaa acaactccag taatgctcca cttagctgat ggtggcgata caggagcatt
    55681 tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc
    55741 gtattatccg tattggcacg gttcaatgaa tgaacttcaa accaatatga atgcgatcag
    55801 ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct tatgggcata caacagcgaa
    55861 tgccgatact atgccaaatg cttttggtga agccgaagca gctgcggggg gatatgaacc
    55921 aacaccgaca ggacaagcag agtatttgct ggatcttgcc gatcggattc aggcagtacc
    55981 gaataatcgc ggggcgggct tcttctattg ggaacctctt tggtacaacg gaaatgtgag
    56041 ttgggcaaca caagcaggta tgaattatct cagtgttcaa tccactatgg gaaacgaatg
    56101 ggacaaccaa gcgatgtttg attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa
    56161 acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg atggaccaac
    56221 gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg gtacgatcaa
    56281 aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg atcaagcata
    56341 cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca cattgagtgc
    56401 ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa attatggtgg
    56461 agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga
    56521 cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt caggaaatgc
    56581 ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc gtttcgattc
    56641 aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac
    56701 ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa aacgagtaaa
    56761 gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat ttcgtaagaa
    56821 gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa gaaagggtaa
    56881 ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa aagttctcgg
    56941 aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa ttaacttata
    57001 ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc attttagcta
    57061 ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct ttattcccac
    57121 tctgttttca acgtaaagtg tataaaacag aatgcgatac tatcaaggga gttaaaacaa
    57181 tatacagaac agtaaaacac tataaataaa aataaaagtg caataaaagt gcaatttttg
    57241 cacctaatca tactctgttt atatatgaaa actagattct gaatccaata tttggttaac
    57301 gagaggcgtt cgaatttgaa cgtcttttat atattacttt caaataaata tgaggtagaa
    57361 tactatctat tttcttttta acccaattgt tataatggag agtgtaaaat attttgtgta
    57421 aatagaaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaagag
    57481 aggacttccc tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg
    57541 atttgaatga attattccgt gtccatttgg agaaagcagt caatacgctt ctcaaaacgg
    57601 agttaacggc tttcctagat tacgaaaagt atgatcgcat tggttttaac acgggtaatt
    57661 ctcgtaacgg ctcctatgac cgtacggtca agaccgagta tggggaactt catctccaga
    57721 ttccgcgcga ccgcaatggt gagttcaagc aacagactgt tcctgcttat agacggacga
    57781 atgacacgtt agaggagacc gtcattcacc tcttccgaaa aggtattacc atgtcggaaa
    57841 tcgcagactt gattgagaaa atgtatgggc attactacac gccccaaacc atgtccaata
    57901 taacaaaatc atttacagaa gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt
    57961 atgctgctat ttatatggac gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg
    58021 aagctattca tatcgcagtt ggcattcgcc cggacggatc aaaggaagta ttgagctatg
    58081 cgattgcacc gactgaatcc atcacgattt gggaggaaat tttattggac cttcaagagc
    58141 gcggtttgaa aaatgtcctc ctgttcatca cggatggctt aaaggggatg gtaggagcga
    58201 ttagtcggtt ctatcccaaa gctcgttttc aacattgttg tgtacacgtt tcccgtaata
    58261 tcagtcataa agtgcgtgtc gatgatcgta aggaagtctg tgatgatttt aaaatggtgt
    58321 atcaagcctc atctaaagag gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga
    58381 aaaccagcta tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg ctcactttct
    58441 atgatttccc cttggccata cgcaagagta tttattctac gaacttgatt gaatccttta
    58501 ataaacaaat caagaaatac agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg
    58561 aacgtttctt agtctcgtct tttgatactt acaaccaaaa attcctaggt cgcagtcata
    58621 aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg attgagaatt
    58681 agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct cccttataat
    58741 gagaactttt actaacataa tttatgataa acttactata taatcatatt aattaaatag
    58801 ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag aaattatgaa
    58861 tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa tgattcaggg
    58921 aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta ctatagtagt
    58981 aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca agggcattgg
    59041 attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa tgagatttgt
    59101 gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat ttcaaatgca
    59161 gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat tagaaagcaa
    59221 ttatttgata tttccactga attagactct catcaattta acgaatttag aaattctatt
    59281 cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt ctgtatcaat
    59341 gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa
    59401 gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg
    59461 gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca
    59521 gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta
    59581 aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt
    59641 ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt
    59701 cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag
    59761 ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat gatttattta
    59821 gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg
    59881 attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta
    59941 aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa
    60001 gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg
    60061 aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat
    60121 ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa
    60181 ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa
    60241 ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg
    60301 tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta
    60361 actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa
    60421 gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt
    60481 aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat
    60541 attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact
    60601 gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa
    60661 ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt
    60721 ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta
    60781 gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca
    60841 gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca
    60901 ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag
    60961 atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa
    61021 aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt
    61081 atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc
    61141 aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag
    61201 ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt
    61261 gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc
    61321 tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga gcttacgtca
    61381 gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac aaaattgatt
    61441 tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt tctgaaatgt
    61501 atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta ctagtagcgt
    61561 ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgctagtaat ataagggcga
    61621 tttatgtgga tgaatttcaa gatactagag aattacaata caatgtaata gctaaattac
    61681 ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa gcaatttatg
    61741 gatcattaga tggtatagct aaatcagttg aagaactaga aatgctaact agtcacactt
    61801 ttcaatcaaa gacgctacat ggctgttaca ggtctaatca gagattagta gacttctact
    61861 ctaattttca gagttgtcct tacgagattg aaagcagagg gaataataaa aacgaccgtg
    61921 gttttataag ttatgattgc aaagttacta aggaggattt acccaacata ataaaaaata
    61981 taataaagga aaagattgaa agcggcattc atgaaaaaga tatttgtgta cttgctcctc
    62041 aacactatcc tctgttttca ctgggtgagt atttaaagaa agaattaccg tattgcaatt
    62101 ttgatgctat aaatatatca ccaattaagg taaataatca tagtttattt ttcaagttag
    62161 caattttgta ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg
    62221 atattttaat t
//
LOCUS       DANJJW010000006        59007 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000006 DANJJW010000000
VERSION     DANJJW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            198..1079
                     /locus_tag="PWS82_000361"
     CDS             198..1079
                     /locus_tag="PWS82_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905519.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1092..2702
                     /locus_tag="PWS82_000362"
     CDS             1092..2702
                     /locus_tag="PWS82_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL0905520.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2779..3438)
                     /locus_tag="PWS82_000363"
     CDS             complement(2779..3438)
                     /locus_tag="PWS82_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0905521.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3721..4386)
                     /locus_tag="PWS82_000364"
     CDS             complement(3721..4386)
                     /locus_tag="PWS82_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL0905522.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4533..5138)
                     /gene="dhaL"
                     /locus_tag="PWS82_000365"
     CDS             complement(4533..5138)
                     /gene="dhaL"
                     /locus_tag="PWS82_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL0905523.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5166..6176)
                     /gene="dhaK"
                     /locus_tag="PWS82_000366"
     CDS             complement(5166..6176)
                     /gene="dhaK"
                     /locus_tag="PWS82_000366"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL0905524.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6151..6597)
                     /gene="dhaM"
                     /locus_tag="PWS82_000367"
     CDS             complement(6151..6597)
                     /gene="dhaM"
                     /locus_tag="PWS82_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL0905525.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6700..7020
                     /locus_tag="PWS82_000368"
     CDS             6700..7020
                     /locus_tag="PWS82_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL0905526.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7132..8001)
                     /locus_tag="PWS82_000369"
     CDS             complement(7132..8001)
                     /locus_tag="PWS82_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0905527.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8097..9400)
                     /locus_tag="PWS82_000370"
                     /pseudo
     CDS             complement(8097..9400)
                     /locus_tag="PWS82_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9397..9906)
                     /locus_tag="PWS82_000371"
     CDS             complement(9397..9906)
                     /locus_tag="PWS82_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905528.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10081..12216)
                     /locus_tag="PWS82_000372"
     CDS             complement(10081..12216)
                     /locus_tag="PWS82_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0905529.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12299..13954)
                     /locus_tag="PWS82_000373"
     CDS             complement(12299..13954)
                     /locus_tag="PWS82_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0905530.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13956..14270)
                     /locus_tag="PWS82_000374"
     CDS             complement(13956..14270)
                     /locus_tag="PWS82_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0905531.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14440..14700
                     /locus_tag="PWS82_000375"
     CDS             14440..14700
                     /locus_tag="PWS82_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL0905532.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14714..15028
                     /locus_tag="PWS82_000376"
     CDS             14714..15028
                     /locus_tag="PWS82_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL0905533.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15758..16312)
                     /locus_tag="PWS82_000377"
     CDS             complement(15758..16312)
                     /locus_tag="PWS82_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL0905534.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16438..18366)
                     /locus_tag="PWS82_000378"
     CDS             complement(16438..18366)
                     /locus_tag="PWS82_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0905535.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18672..19388)
                     /locus_tag="PWS82_000379"
     CDS             complement(18672..19388)
                     /locus_tag="PWS82_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0905536.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19389..21212)
                     /gene="glpO"
                     /locus_tag="PWS82_000380"
     CDS             complement(19389..21212)
                     /gene="glpO"
                     /locus_tag="PWS82_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL0905537.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21226..22722)
                     /gene="glpK"
                     /locus_tag="PWS82_000381"
     CDS             complement(21226..22722)
                     /gene="glpK"
                     /locus_tag="PWS82_000381"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL0905538.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22858..24318)
                     /locus_tag="PWS82_000382"
     CDS             complement(22858..24318)
                     /locus_tag="PWS82_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905539.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24308..25603)
                     /locus_tag="PWS82_000383"
     CDS             complement(24308..25603)
                     /locus_tag="PWS82_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0905540.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25935..26612)
                     /locus_tag="PWS82_000384"
     CDS             complement(25935..26612)
                     /locus_tag="PWS82_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL0905541.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26642..27733)
                     /locus_tag="PWS82_000385"
     CDS             complement(26642..27733)
                     /locus_tag="PWS82_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL0905542.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27834..28268)
                     /locus_tag="PWS82_000386"
     CDS             complement(27834..28268)
                     /locus_tag="PWS82_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0905543.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28852..30309)
                     /gene="ascB"
                     /locus_tag="PWS82_000387"
     CDS             complement(28852..30309)
                     /gene="ascB"
                     /locus_tag="PWS82_000387"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0905544.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30306..32201)
                     /locus_tag="PWS82_000388"
     CDS             complement(30306..32201)
                     /locus_tag="PWS82_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0905545.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32259..34034)
                     /locus_tag="PWS82_000389"
     CDS             complement(32259..34034)
                     /locus_tag="PWS82_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0905546.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34084..35580)
                     /locus_tag="PWS82_000390"
     CDS             complement(34084..35580)
                     /locus_tag="PWS82_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0905547.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35774..37396)
                     /locus_tag="PWS82_000391"
     CDS             complement(35774..37396)
                     /locus_tag="PWS82_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0905548.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37845..39158)
                     /locus_tag="PWS82_000392"
     CDS             complement(37845..39158)
                     /locus_tag="PWS82_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0905549.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39403..39627)
                     /locus_tag="PWS82_000393"
     CDS             complement(39403..39627)
                     /locus_tag="PWS82_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905550.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39647..40069)
                     /locus_tag="PWS82_000394"
     CDS             complement(39647..40069)
                     /locus_tag="PWS82_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL0905551.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40213..40923)
                     /locus_tag="PWS82_000395"
     CDS             complement(40213..40923)
                     /locus_tag="PWS82_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905552.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40941..41438)
                     /locus_tag="PWS82_000396"
     CDS             complement(40941..41438)
                     /locus_tag="PWS82_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905553.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41475..41927)
                     /locus_tag="PWS82_000397"
     CDS             complement(41475..41927)
                     /locus_tag="PWS82_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0905554.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(42062..42667)
                     /locus_tag="PWS82_000398"
     CDS             complement(42062..42667)
                     /locus_tag="PWS82_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0905555.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43117..43737)
                     /locus_tag="PWS82_000399"
     CDS             complement(43117..43737)
                     /locus_tag="PWS82_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0905556.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43739..44608)
                     /locus_tag="PWS82_000400"
     CDS             complement(43739..44608)
                     /locus_tag="PWS82_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL0905557.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44631..45038)
                     /locus_tag="PWS82_000401"
     CDS             complement(44631..45038)
                     /locus_tag="PWS82_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0905558.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(45057..46346)
                     /locus_tag="PWS82_000402"
     CDS             complement(45057..46346)
                     /locus_tag="PWS82_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0905559.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46373..46675)
                     /locus_tag="PWS82_000403"
     CDS             complement(46373..46675)
                     /locus_tag="PWS82_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0905560.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46700..48793)
                     /locus_tag="PWS82_000404"
     CDS             complement(46700..48793)
                     /locus_tag="PWS82_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0905561.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49282..50046)
                     /locus_tag="PWS82_000405"
     CDS             complement(49282..50046)
                     /locus_tag="PWS82_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL0905562.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50157..51026)
                     /gene="mdcB"
                     /locus_tag="PWS82_000406"
     CDS             complement(50157..51026)
                     /gene="mdcB"
                     /locus_tag="PWS82_000406"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL0905563.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(51051..52127)
                     /locus_tag="PWS82_000407"
     CDS             complement(51051..52127)
                     /locus_tag="PWS82_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL0905564.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52151..52744)
                     /locus_tag="PWS82_000408"
     CDS             complement(52151..52744)
                     /locus_tag="PWS82_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL0905565.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52748..54424)
                     /gene="mdcD"
                     /locus_tag="PWS82_000409"
     CDS             complement(52748..54424)
                     /gene="mdcD"
                     /locus_tag="PWS82_000409"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL0905566.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54414..54713)
                     /locus_tag="PWS82_000410"
     CDS             complement(54414..54713)
                     /locus_tag="PWS82_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL0905567.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54815..56461)
                     /gene="mdcA"
                     /locus_tag="PWS82_000411"
     CDS             complement(54815..56461)
                     /gene="mdcA"
                     /locus_tag="PWS82_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL0905568.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56468..57379)
                     /locus_tag="PWS82_000412"
     CDS             complement(56468..57379)
                     /locus_tag="PWS82_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0905569.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57499..58356
                     /locus_tag="PWS82_000413"
     CDS             57499..58356
                     /locus_tag="PWS82_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0905570.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58543..58905)
                     /locus_tag="PWS82_000414"
     CDS             complement(58543..58905)
                     /locus_tag="PWS82_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL0905571.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT        16598 a        12568 c        10138 g        19703 t
ORIGIN      
        1 tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct tgtttatgaa
       61 ttcattgaca gaaatttata aaaagaatat tatgtactaa aataataaaa aaagttttat
      121 aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat atttttagaa
      181 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
      241 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      301 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      361 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      421 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      481 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      541 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      601 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      661 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      721 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      781 gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa ggaacactcg
      841 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      901 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      961 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
     1021 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
     1081 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
     1141 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1201 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1261 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1321 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1381 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1441 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1501 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1561 cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1621 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1681 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1741 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1801 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1861 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1921 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1981 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     2041 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     2101 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
     2161 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2221 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2281 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2341 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2401 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2461 ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2521 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2581 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2641 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2701 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2761 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2821 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2881 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2941 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     3001 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     3061 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     3121 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3181 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3241 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3301 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3361 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3421 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3481 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3541 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3601 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3661 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3721 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3781 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3841 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3901 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3961 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     4021 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     4081 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     4141 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4201 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4261 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4321 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4381 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4441 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4501 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4561 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4621 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4681 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4741 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4801 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4861 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4921 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4981 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     5041 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     5101 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     5161 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5221 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5281 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5341 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5401 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5461 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5521 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5581 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5641 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5701 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5761 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5821 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atccgcttcc
     5881 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5941 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     6001 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     6061 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     6121 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6181 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6241 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6301 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6361 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6421 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6481 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6541 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6601 tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta gtgcttcttg
     6661 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6721 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6781 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6841 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6901 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6961 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     7021 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     7081 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     7141 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7201 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7261 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7321 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7381 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7441 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7501 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7561 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7621 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7681 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7741 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7801 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7861 agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7921 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7981 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     8041 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     8101 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     8161 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8221 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8281 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8341 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8401 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8461 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8521 cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat cttcaacgtc
     8581 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8641 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8701 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8761 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8821 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8881 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8941 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     9001 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     9061 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     9121 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc ttcgatcaca
     9181 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9241 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9301 catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata gacaaacaag
     9361 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
     9421 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
     9481 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
     9541 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
     9601 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
     9661 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
     9721 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
     9781 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
     9841 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
     9901 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
     9961 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
    10021 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
    10081 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
    10141 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    10201 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    10261 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    10321 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    10381 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    10441 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    10501 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    10561 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    10621 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    10681 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    10741 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    10801 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    10861 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    10921 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    10981 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    11041 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    11101 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    11161 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    11221 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    11281 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    11341 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    11401 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    11461 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    11521 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    11581 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    11641 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    11701 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    11761 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    11821 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    11881 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    11941 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    12001 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    12061 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    12121 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    12181 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    12241 tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    12301 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    12361 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    12421 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    12481 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    12541 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    12601 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    12661 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    12721 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    12781 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    12841 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    12901 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    12961 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    13021 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    13081 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    13141 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    13201 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    13261 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    13321 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    13381 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    13441 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    13501 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    13561 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    13621 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    13681 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    13741 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    13801 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    13861 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    13921 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    13981 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    14041 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    14101 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    14161 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    14221 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    14281 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    14341 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    14401 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    14461 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    14521 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    14581 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    14641 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    14701 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    14761 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    14821 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    14881 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    14941 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    15001 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    15061 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    15121 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    15181 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    15241 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    15301 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaaggaa
    15361 gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggattt
    15421 ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat accgtttaag
    15481 gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagc
    15541 tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15601 ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt caaaaacaac
    15661 ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg agtctattta
    15721 tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga aacatctttc
    15781 ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa
    15841 tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc atgccaacat
    15901 agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg atatttttag
    15961 atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat agaccaaaca
    16021 acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt
    16081 tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat ctccgagcaa
    16141 tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct gctatataaa
    16201 atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg aatatcgata
    16261 aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc attttatcgc
    16321 cctacctcct cagcactctt ttctttttat tttagcttac acggggaata aaaccaatga
    16381 tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt gagataatca
    16441 cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt cttttactag
    16501 tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac gtgttttata
    16561 atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac
    16621 cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata catcaatcgt
    16681 atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga caaatatccg
    16741 atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg taactgctgt
    16801 cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt aacgctctgc
    16861 ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag cttggctttc
    16921 tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat
    16981 agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt
    17041 cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc
    17101 agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct
    17161 ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc
    17221 attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc
    17281 tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac
    17341 tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata
    17401 gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc
    17461 ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg
    17521 gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc
    17581 gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc
    17641 actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg
    17701 ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt
    17761 tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc
    17821 ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc
    17881 tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact
    17941 gactttatta ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag
    18001 atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt
    18061 gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat
    18121 caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac
    18181 atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac
    18241 agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat
    18301 tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt
    18361 gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag
    18421 ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa
    18481 agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg
    18541 gctattcgac ttactgatta gataccagct acactcacaa aacatacccc attcttctag
    18601 gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa actttagtat
    18661 gttttataga tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt
    18721 ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc
    18781 aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt
    18841 ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt
    18901 ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca
    18961 attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt
    19021 cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca
    19081 agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc
    19141 caactaattg cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg
    19201 cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg
    19261 acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca
    19321 tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg agtcatatca
    19381 ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct
    19441 aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca
    19501 tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta
    19561 cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac
    19621 cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag
    19681 atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa
    19741 ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg
    19801 aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat
    19861 tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg
    19921 gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct
    19981 ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct
    20041 tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta
    20101 ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta
    20161 ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt
    20221 gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact
    20281 ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt
    20341 tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt
    20401 tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt
    20461 ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc
    20521 gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct
    20581 ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact
    20641 agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg
    20701 ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct
    20761 ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt
    20821 gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct
    20881 ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata
    20941 ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc
    21001 agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa
    21061 tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg
    21121 gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga
    21181 atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg
    21241 ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat
    21301 ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca
    21361 ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc
    21421 tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt
    21481 tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt
    21541 atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc
    21601 ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg
    21661 agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt
    21721 cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg
    21781 aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat
    21841 gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt
    21901 catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat
    21961 ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc
    22021 ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa
    22081 catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat
    22141 attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt
    22201 cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg
    22261 ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc
    22321 atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt
    22381 atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc
    22441 tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat
    22501 ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt
    22561 ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc
    22621 tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt
    22681 tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat
    22741 atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca
    22801 aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca
    22861 attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga
    22921 cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac
    22981 caaatcatat tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag
    23041 ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat
    23101 tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg
    23161 gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc
    23221 tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg
    23281 ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt
    23341 gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg
    23401 acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt
    23461 tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac
    23521 aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag
    23581 atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt
    23641 cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc
    23701 gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc
    23761 caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc
    23821 ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta
    23881 taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat
    23941 ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac
    24001 ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa
    24061 caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt
    24121 tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata
    24181 ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt
    24241 tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa
    24301 taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac
    24361 ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt
    24421 ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg
    24481 ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta
    24541 ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct
    24601 cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac
    24661 gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg
    24721 tacggacagc gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta
    24781 cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta
    24841 cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcggggc
    24901 gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa
    24961 tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt
    25021 gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa
    25081 ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta
    25141 tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt
    25201 gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag
    25261 aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt
    25321 tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt
    25381 aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt
    25441 cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg
    25501 attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg
    25561 catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt
    25621 ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca
    25681 accttatgat acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt
    25741 ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt
    25801 tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata
    25861 ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga
    25921 cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca
    25981 gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct
    26041 gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact
    26101 tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca
    26161 gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa
    26221 acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt
    26281 gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca
    26341 tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca
    26401 tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt
    26461 acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca
    26521 taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt
    26581 tctacatttg ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc
    26641 gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat
    26701 atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg
    26761 cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata
    26821 cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt
    26881 gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta
    26941 ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt
    27001 caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga
    27061 aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg
    27121 ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc
    27181 gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact
    27241 cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg
    27301 tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg
    27361 caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc
    27421 caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct
    27481 cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc
    27541 cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt
    27601 tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca
    27661 taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat
    27721 caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc
    27781 tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt
    27841 ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt
    27901 cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca
    27961 aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg
    28021 acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag
    28081 agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata
    28141 aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac
    28201 atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc
    28261 ttttccattt catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca
    28321 aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct
    28381 tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt
    28441 gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat
    28501 ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag
    28561 gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    28621 tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga
    28681 ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc
    28741 atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg
    28801 taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat
    28861 ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt
    28921 ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat
    28981 ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc
    29041 tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat agtcaatgcg
    29101 gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc
    29161 agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc
    29221 cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc
    29281 agttattaag tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga
    29341 catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc
    29401 tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata
    29461 atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt
    29521 ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc
    29581 gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa
    29641 ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac
    29701 tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata
    29761 ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat
    29821 aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg
    29881 ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata
    29941 aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca
    30001 tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt
    30061 aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc
    30121 ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt
    30181 aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc
    30241 ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa
    30301 ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga
    30361 ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca
    30421 tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact
    30481 ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata
    30541 ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt
    30601 ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc cgttggaatg
    30661 atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa
    30721 tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg
    30781 atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa
    30841 taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt
    30901 cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct
    30961 ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca
    31021 ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc
    31081 ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc
    31141 atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt
    31201 acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca
    31261 ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt
    31321 gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac gatgatacaa
    31381 atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca
    31441 atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct
    31501 agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc
    31561 gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt
    31621 tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca
    31681 ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg
    31741 agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg
    31801 gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta
    31861 gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc atttactgca
    31921 tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt
    31981 tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt
    32041 gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc
    32101 gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt
    32161 cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttata
    32221 atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact
    32281 gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc
    32341 ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt
    32401 tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact
    32461 aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg gatttttatt
    32521 tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg
    32581 gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta
    32641 ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca
    32701 tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat
    32761 tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta
    32821 attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg
    32881 gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt
    32941 gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca
    33001 aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt
    33061 agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc
    33121 gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc
    33181 aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa
    33241 tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta
    33301 tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga
    33361 gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt
    33421 aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggcaac
    33481 cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt
    33541 tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg
    33601 cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag
    33661 aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca
    33721 atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg
    33781 atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac
    33841 tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt
    33901 ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt
    33961 cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc
    34021 tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa
    34081 aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt
    34141 ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct
    34201 atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc
    34261 gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat
    34321 ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc
    34381 atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg
    34441 gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg
    34501 ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac
    34561 gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc
    34621 tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc
    34681 ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa
    34741 aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata
    34801 tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat
    34861 tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata
    34921 aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac
    34981 caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca
    35041 atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc
    35101 gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata
    35161 atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc
    35221 ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc
    35281 ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc
    35341 ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa
    35401 agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat
    35461 cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca
    35521 ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat
    35581 aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac
    35641 aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt
    35701 taatatttat atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat
    35761 ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt
    35821 tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg
    35881 gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt
    35941 catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc
    36001 cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa
    36061 cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc
    36121 gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg
    36181 tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct tttgtaaatc
    36241 cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat
    36301 catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt
    36361 tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat
    36421 ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc
    36481 cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca
    36541 agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt
    36601 ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg atttcggtag
    36661 ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca
    36721 tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt
    36781 tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat
    36841 tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac
    36901 gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac
    36961 cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat
    37021 aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat
    37081 cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt
    37141 caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac
    37201 tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat
    37261 caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac
    37321 catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc
    37381 accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca
    37441 tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt
    37501 atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa
    37561 ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact
    37621 cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat tttttcgttt
    37681 ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac
    37741 tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt
    37801 tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt cacattcaat
    37861 atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga tacttctttt
    37921 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    37981 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    38041 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    38101 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    38161 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    38221 gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc cgcaagcata
    38281 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    38341 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    38401 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    38461 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    38521 aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat gccacctggt
    38581 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    38641 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    38701 tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc atatttcaaa
    38761 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    38821 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    38881 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    38941 cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt cgcagtaaac
    39001 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    39061 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    39121 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    39181 cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata
    39241 tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc
    39301 atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt
    39361 ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat
    39421 tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt
    39481 atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc
    39541 tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata
    39601 accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa tcagacgact
    39661 cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa gtcaagatgc
    39721 cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat
    39781 ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa
    39841 atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa
    39901 attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc
    39961 ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcattt
    40021 gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa
    40081 agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt
    40141 gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc cgctcttctt
    40201 atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact
    40261 agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg
    40321 tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt
    40381 cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc
    40441 atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa
    40501 tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc
    40561 tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt
    40621 gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg
    40681 tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt
    40741 ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa
    40801 ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa
    40861 gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt
    40921 catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga
    40981 tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa
    41041 gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct
    41101 tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt
    41161 tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc
    41221 gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa
    41281 tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag
    41341 taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata
    41401 ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc
    41461 acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg
    41521 ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa
    41581 agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt
    41641 tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa
    41701 cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct
    41761 actttgatca tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact
    41821 aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa
    41881 tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt
    41941 tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat tttcaataaa
    42001 aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag
    42061 ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc
    42121 gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat
    42181 tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat
    42241 actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag
    42301 tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag
    42361 acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga
    42421 ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat
    42481 gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac
    42541 tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt
    42601 ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct
    42661 tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga
    42721 ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa
    42781 aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc
    42841 ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc
    42901 tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa cggtgttctc
    42961 ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct gtttttttga
    43021 aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt aaacagcgtt
    43081 caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat tgatttcttt
    43141 ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta catcaaattc
    43201 atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct ttttgaatgc
    43261 ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac catagtgata
    43321 atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt accgacgttg
    43381 acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc cgtatctatc
    43441 tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg acacaatcac
    43501 tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat aaactttcgt
    43561 cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg catagtattc
    43621 cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta gtttattcat
    43681 aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact ttttcatctc
    43741 atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc ttatgcttga
    43801 caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac ttgttgattt
    43861 ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc tccgttccaa
    43921 cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct ttcacacctg
    43981 ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc agcaagggaa
    44041 tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca atagctaatg
    44101 catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta tataccttgt
    44161 cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt tctccttcta
    44221 cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg atattttctt
    44281 tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg attgcttctt
    44341 tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc atttcttctt
    44401 ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta gcggtatcga
    44461 cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa
    44521 gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct tctgctgttt
    44581 ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt ttatttgttt
    44641 cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc catgtagtaa
    44701 tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt atagaggctc
    44761 atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt ttctaaatat
    44821 tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg ttcataaagt
    44881 ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg tgtaatctct
    44941 gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc cagaactttt
    45001 ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac tttcaattaa
    45061 aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc tactgtatct
    45121 acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac tagcaaagcg
    45181 ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc tcggcgtccg
    45241 ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt tagcatacca
    45301 ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat cgtaccaatc
    45361 gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa aactggacaa
    45421 tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa tgccggcacg
    45481 atttcaccta acagtagacg tacaccagct agcaatacat acacacccac tacgaattgg
    45541 atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc agaagcgaat
    45601 ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac catgacagac
    45661 aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt
    45721 ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa gtacccgatc
    45781 gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat gatcggttgc
    45841 gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac caagatcaat
    45901 acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc catccataaa
    45961 attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa aataatattg
    46021 aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa atcattcatt
    46081 gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa tccttcagta
    46141 aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc actcaacact
    46201 tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga tttcttctgc
    46261 agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt caaaatattt
    46321 tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt atttagataa
    46381 ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt gaaacaatat
    46441 ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac tgcgaacgaa
    46501 aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa atatcacaat
    46561 ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac atctgtgctg
    46621 caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg ttcatctttg
    46681 ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt ttgaaatctt
    46741 cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc aattcaatta
    46801 gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa aaaataatct
    46861 ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta gctacgctga
    46921 ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg tgtttgccaa
    46981 tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca tagcgctctt
    47041 cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc tcccaagtag
    47101 tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac ataggctgga
    47161 tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac ttgccataga
    47221 tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga gtgaataact
    47281 ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt ttcaaaaagt
    47341 cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa agcggcttat
    47401 ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta tctactaatt
    47461 cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt tctttcagat
    47521 tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact gatttataaa
    47581 cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg acaagaaaag
    47641 ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga acaaaatgag
    47701 tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat ggattcatga
    47761 cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag agattttcga
    47821 ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca aattggatca
    47881 attggatcgt tagtaattga gcttgtaccc actcgattga attggagata tctctagcat
    47941 tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca ttgtccaatt
    48001 gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt atttctgtat
    48061 ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc cagatcaatg
    48121 taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac ttattggaat
    48181 agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag attttttgag
    48241 ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga
    48301 taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct
    48361 ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat
    48421 cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg
    48481 ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa
    48541 agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct
    48601 tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc
    48661 agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg aactcacttg
    48721 ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg
    48781 ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa
    48841 taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa
    48901 taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat
    48961 tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    49021 caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta tcgcagatgc
    49081 aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc ttgagcgata
    49141 agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag
    49201 gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac
    49261 tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat cggcttgcca
    49321 tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt
    49381 cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa
    49441 atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat
    49501 agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg
    49561 atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc
    49621 ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg ctttcttttc
    49681 tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct tttgtctttt
    49741 attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa
    49801 tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat aagaatctct
    49861 gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa atgaacctct
    49921 gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg
    49981 ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt
    50041 tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat agccttgcta
    50101 aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg tcttatttag
    50161 tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc tatcttcagc
    50221 atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg gttttcctga
    50281 caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact agcagcatga
    50341 gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac gcatgtgtca
    50401 tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata tgaatcatta
    50461 tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa tatcgcttct
    50521 tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt ggttttttta
    50581 tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag tattttcgat
    50641 aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa tagccccatt
    50701 gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat tgccttttcc
    50761 gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc atgatgccat
    50821 gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt aagaaataaa
    50881 gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc aggttttgga
    50941 cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg ctgagtcact
    51001 gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta ttacttgctg
    51061 aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc aaatgcacaa
    51121 cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg cagccaacta
    51181 tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata cgtacgataa
    51241 gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac aatcattttg
    51301 gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg gatcgtattc
    51361 aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga acggacagaa
    51421 aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc caaatattga
    51481 tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact agcaacaaac
    51541 cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt ttgatagaac
    51601 agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac atatttttta
    51661 ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg gatctgcagc
    51721 atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt cagtaaaaag
    51781 cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca aagataagcc
    51841 gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa aaattcccaa
    51901 taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat aaacattggt
    51961 gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt tgcgctgtgg
    52021 ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac agctgatact
    52081 ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc tttctctctc
    52141 tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt ttcaatagaa
    52201 tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc cgagtcacta
    52261 cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga attggcaatt
    52321 caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt actgccggaa
    52381 tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat cctgataata
    52441 tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc tcttgtctat
    52501 acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca tgttttgaga
    52561 tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt agtatttctg
    52621 cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga acgacccatt
    52681 caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca tgtgcttgaa
    52741 tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt gtttcgacac
    52801 gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg tcatttgtat
    52861 tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca ttttgttctg
    52921 aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt tttgtataat
    52981 tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt tcagcaaccg
    53041 aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta atttctttat
    53101 tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca cctgaaatag
    53161 catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt gcataagcag
    53221 cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag ccgtatgctt
    53281 ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct ttatcttctt
    53341 cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca acttcaccgt
    53401 ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg atggcttctt
    53461 ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt ggatttttca
    53521 tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt tcatttcctt
    53581 ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct tcattgtaat
    53641 cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa taaaatgctg
    53701 agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca gcagctttga
    53761 ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc cgttgacgag
    53821 atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga acgcctgctt
    53881 cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt gcgattaaat
    53941 aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt cctggaatca
    54001 aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt tctgaaatag
    54061 ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt acaatgactg
    54121 gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca gcaacgattt
    54181 ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata gcgattacta
    54241 ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca ctttcaggga
    54301 cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca atcaattcac
    54361 gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca aaactatttt
    54421 tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc tggtgtagca
    54481 ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa tctcgttaat
    54541 acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac agtagattct
    54601 gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac tcctacatgg
    54661 attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc catggatctc
    54721 tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt cttccatctg
    54781 ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg aatttcattg
    54841 gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt gcagctaata
    54901 atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc acgatgccgg
    54961 ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt gtaacaccgc
    55021 caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac aaataagcaa
    55081 ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact ggtgcattgg
    55141 ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag actggttttt
    55201 tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc cctaatggat
    55261 catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg ccaggattat
    55321 gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg gtagaagagt
    55381 ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca tattgtcctg
    55441 cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca aaaaagacat
    55501 ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta tggatgcttt
    55561 caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat acccagttct
    55621 tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac aattcgatag
    55681 ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct gcgtagattc
    55741 ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga ggatctcgtg
    55801 tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct acccagtcac
    55861 cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg atcacgatac
    55921 catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg aaacctgtgt
    55981 acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt ggaatcaaat
    56041 cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat ttcattgtgc
    56101 cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag aaatcgatct
    56161 tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata ctagacatga
    56221 tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct gaaagaaaac
    56281 ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca atcagttttt
    56341 ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag ttgctgattt
    56401 tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt ggtttttcca
    56461 taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca tgttggtcta
    56521 gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc tttgtcaaag
    56581 tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc aagtccatca
    56641 tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga atctcttctg
    56701 cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca tcagatagat
    56761 ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag agacagtgag
    56821 atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg gcttctgcca
    56881 acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga caattttcat
    56941 ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc tcagcttcgc
    57001 ttctcgtcac acctactact acgcccattg cataaccgct tggataagct tctttcatta
    57061 agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat cgacaagcaa
    57121 cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga ataccgtttt
    57181 tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg ataaaaaccg
    57241 tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt tctaaacgtt
    57301 ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct tgtccaggaa
    57361 ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc actgctatct
    57421 tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa caaaaaggta
    57481 tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa agtattcgaa
    57541 acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc cttgagccgt
    57601 gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag cggcagaaac
    57661 attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac attagacaca
    57721 ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac ttccgtcata
    57781 ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt tatggaaaag
    57841 catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat taccgatcct
    57901 gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat catgaaagaa
    57961 cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca tctgcaatta
    58021 gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt ccgtcataca
    58081 ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac gacagatgat
    58141 cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt cccaaaagtt
    58201 tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga tttgcccttg
    58261 acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat caaaatgatt
    58321 gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg ggacaaaatt
    58381 cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt agaaaataag
    58441 ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    58501 taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg ttgatttaaa
    58561 acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta gatcaataga
    58621 gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc ctcgcaaaat
    58681 tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat ggtcacttgc
    58741 atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt ttttgctttt
    58801 ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg gagacaaatt
    58861 gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct tccatctctt
    58921 tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga atacaagtag
    58981 ttgctaatca aatacttcga tattttt
//
LOCUS       DANJJW010000007        54972 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000007 DANJJW010000000
VERSION     DANJJW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            399..3206
                     /locus_tag="PWS82_000415"
     CDS             399..3206
                     /locus_tag="PWS82_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0905572.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3397..3888
                     /locus_tag="PWS82_000416"
     CDS             3397..3888
                     /locus_tag="PWS82_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0905573.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3991..4980
                     /gene="manX"
                     /locus_tag="PWS82_000417"
     CDS             3991..4980
                     /gene="manX"
                     /locus_tag="PWS82_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL0905574.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5013..5816
                     /locus_tag="PWS82_000418"
     CDS             5013..5816
                     /locus_tag="PWS82_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL0905575.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5837..6751
                     /locus_tag="PWS82_000419"
     CDS             5837..6751
                     /locus_tag="PWS82_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL0905576.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6834..7208
                     /locus_tag="PWS82_000420"
     CDS             6834..7208
                     /locus_tag="PWS82_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL0905577.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7347..7592
                     /locus_tag="PWS82_000421"
     CDS             7347..7592
                     /locus_tag="PWS82_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0905578.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7764..8828
                     /locus_tag="PWS82_000422"
     CDS             7764..8828
                     /locus_tag="PWS82_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0905579.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9074..9499
                     /locus_tag="PWS82_000423"
     CDS             9074..9499
                     /locus_tag="PWS82_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0905580.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9492..9941
                     /gene="nusB"
                     /locus_tag="PWS82_000424"
     CDS             9492..9941
                     /gene="nusB"
                     /locus_tag="PWS82_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL0905581.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10038..10892
                     /locus_tag="PWS82_000425"
     CDS             10038..10892
                     /locus_tag="PWS82_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL0905582.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10893..12230
                     /locus_tag="PWS82_000426"
     CDS             10893..12230
                     /locus_tag="PWS82_000426"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL0905583.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12235..12459
                     /locus_tag="PWS82_000427"
     CDS             12235..12459
                     /locus_tag="PWS82_000427"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL0905584.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12461..13351
                     /locus_tag="PWS82_000428"
     CDS             12461..13351
                     /locus_tag="PWS82_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0905585.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13360..14178
                     /locus_tag="PWS82_000429"
     CDS             13360..14178
                     /locus_tag="PWS82_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL0905586.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14285..14734
                     /locus_tag="PWS82_000430"
     CDS             14285..14734
                     /locus_tag="PWS82_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0905587.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14752..16434
                     /gene="recN"
                     /locus_tag="PWS82_000431"
     CDS             14752..16434
                     /gene="recN"
                     /locus_tag="PWS82_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL0905588.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16489..16605)
                     /locus_tag="PWS82_000432"
     CDS             complement(16489..16605)
                     /locus_tag="PWS82_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL0905589.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16678..17634)
                     /locus_tag="PWS82_000433"
     CDS             complement(16678..17634)
                     /locus_tag="PWS82_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0905590.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17805..18173
                     /locus_tag="PWS82_000434"
     CDS             17805..18173
                     /locus_tag="PWS82_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL0905591.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18343..18774
                     /gene="mraZ"
                     /locus_tag="PWS82_000435"
     CDS             18343..18774
                     /gene="mraZ"
                     /locus_tag="PWS82_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL0905592.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18795..19754
                     /gene="rsmH"
                     /locus_tag="PWS82_000436"
     CDS             18795..19754
                     /gene="rsmH"
                     /locus_tag="PWS82_000436"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL0905593.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19759..20160
                     /gene="ftsL"
                     /locus_tag="PWS82_000437"
     CDS             19759..20160
                     /gene="ftsL"
                     /locus_tag="PWS82_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL0905594.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20161..22353
                     /locus_tag="PWS82_000438"
     CDS             20161..22353
                     /locus_tag="PWS82_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0905595.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22379..23341
                     /locus_tag="PWS82_000439"
     CDS             22379..23341
                     /locus_tag="PWS82_000439"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL0905596.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23355..24716
                     /locus_tag="PWS82_000440"
     CDS             23355..24716
                     /locus_tag="PWS82_000440"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL0905597.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24742..25830
                     /gene="murG"
                     /locus_tag="PWS82_000441"
     CDS             24742..25830
                     /gene="murG"
                     /locus_tag="PWS82_000441"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL0905598.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25845..27065
                     /locus_tag="PWS82_000442"
     CDS             25845..27065
                     /locus_tag="PWS82_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL0905599.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27221..28546
                     /gene="ftsA"
                     /locus_tag="PWS82_000443"
     CDS             27221..28546
                     /gene="ftsA"
                     /locus_tag="PWS82_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL0905600.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28569..29810
                     /gene="ftsZ"
                     /locus_tag="PWS82_000444"
     CDS             28569..29810
                     /gene="ftsZ"
                     /locus_tag="PWS82_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL0905601.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29827..30504
                     /locus_tag="PWS82_000445"
     CDS             29827..30504
                     /locus_tag="PWS82_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL0905602.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30518..31117
                     /locus_tag="PWS82_000446"
     CDS             30518..31117
                     /locus_tag="PWS82_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL0905603.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31132..31419
                     /locus_tag="PWS82_000447"
     CDS             31132..31419
                     /locus_tag="PWS82_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL0905604.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31468..32250
                     /locus_tag="PWS82_000448"
     CDS             31468..32250
                     /locus_tag="PWS82_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL0905605.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32271..32978
                     /locus_tag="PWS82_000449"
     CDS             32271..32978
                     /locus_tag="PWS82_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL0905606.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33240..36038
                     /gene="ileS"
                     /locus_tag="PWS82_000450"
     CDS             33240..36038
                     /gene="ileS"
                     /locus_tag="PWS82_000450"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL0905607.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36087..37613)
                     /locus_tag="PWS82_000451"
     CDS             complement(36087..37613)
                     /locus_tag="PWS82_000451"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL0905608.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37628..38275)
                     /locus_tag="PWS82_000452"
     CDS             complement(37628..38275)
                     /locus_tag="PWS82_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL0905609.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38459..38788)
                     /locus_tag="PWS82_000453"
     CDS             complement(38459..38788)
                     /locus_tag="PWS82_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL0905610.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38965..39693
                     /gene="sfsA"
                     /locus_tag="PWS82_000454"
     CDS             38965..39693
                     /gene="sfsA"
                     /locus_tag="PWS82_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL0905611.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39709..40722
                     /locus_tag="PWS82_000455"
     CDS             39709..40722
                     /locus_tag="PWS82_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0905612.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40722..41999
                     /locus_tag="PWS82_000456"
     CDS             40722..41999
                     /locus_tag="PWS82_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0905613.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42062..44764
                     /locus_tag="PWS82_000457"
     CDS             42062..44764
                     /locus_tag="PWS82_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0905614.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44916..45233
                     /locus_tag="PWS82_000458"
     CDS             44916..45233
                     /locus_tag="PWS82_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0905615.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45263..45583
                     /locus_tag="PWS82_000459"
     CDS             45263..45583
                     /locus_tag="PWS82_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0905616.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45691..47151
                     /locus_tag="PWS82_000460"
     CDS             45691..47151
                     /locus_tag="PWS82_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0905617.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47219..47440
                     /locus_tag="PWS82_000461"
     CDS             47219..47440
                     /locus_tag="PWS82_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905618.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47471..47653
                     /locus_tag="PWS82_000462"
     CDS             47471..47653
                     /locus_tag="PWS82_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL0905619.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47653..48066
                     /locus_tag="PWS82_000463"
     CDS             47653..48066
                     /locus_tag="PWS82_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL0905620.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48189..49370
                     /locus_tag="PWS82_000464"
     CDS             48189..49370
                     /locus_tag="PWS82_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905621.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49441..49605)
                     /locus_tag="PWS82_000465"
     CDS             complement(49441..49605)
                     /locus_tag="PWS82_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905622.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49759..49832
                     /locus_tag="PWS82_000466"
     tRNA            49759..49832
                     /locus_tag="PWS82_000466"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49793..49795,aa:Arg,seq:cct)
     gene            complement(49869..50069)
                     /locus_tag="PWS82_000467"
     CDS             complement(49869..50069)
                     /locus_tag="PWS82_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0905623.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(50374..51606)
                     /locus_tag="PWS82_000468"
     CDS             complement(50374..51606)
                     /locus_tag="PWS82_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL0905624.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            51860..52429
                     /locus_tag="PWS82_000469"
     CDS             51860..52429
                     /locus_tag="PWS82_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0905625.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(52607..53047)
                     /locus_tag="PWS82_000470"
     CDS             complement(52607..53047)
                     /locus_tag="PWS82_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL0905626.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            53205..53969
                     /gene="map"
                     /locus_tag="PWS82_000471"
     CDS             53205..53969
                     /gene="map"
                     /locus_tag="PWS82_000471"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL0905627.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            54001..54924
                     /locus_tag="PWS82_000472"
     CDS             54001..54924
                     /locus_tag="PWS82_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL0905628.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT        18155 a         9365 c        11770 g        15682 t
ORIGIN      
        1 tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta cgaagtttac
       61 tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt ttgataacta
      121 tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta tatggtaaac
      181 agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt tcttttaaat
      241 tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa tagttgacga
      301 aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc tatagaaaag
      361 gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg
      421 ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt gctgaagtgt
      481 tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc gaaggaaaac
      541 tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt acgcacgtaa
      601 agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata
      661 cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac ggcagcatga
      721 aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga ttaaattgtt
      781 tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg tatcattttg
      841 caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat tgtgctgatt
      901 atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta
      961 caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt atgctgtttt
     1021 tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac tttatggatc
     1081 atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg
     1141 taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt cggcgtatcc
     1201 caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag
     1261 taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct ttgacagaag
     1321 atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa
     1381 atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc gaaatctcga
     1441 tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta gcaagcaacc
     1501 gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta gcaccaaatg
     1561 atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta tatgatatca
     1621 tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca atcaatcatt
     1681 tcatttctac agatatcaac atccatctga agtctttcta taaagaccat ggtttttctt
     1741 ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa gtgaccaatc
     1801 agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac tttatttatg
     1861 ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa gaacggcata
     1921 cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct gttgcgaaag
     1981 aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt gaagactatt
     2041 acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga atcggtgtag
     2101 tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc caacagttgt
     2161 tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg aaaacagcat
     2221 tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc atactcttag
     2281 tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact ggaattccag
     2341 tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga aaggtctcag
     2401 tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga ggttacgcag
     2461 aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa aaagctgttt
     2521 tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg atcgaacgag
     2581 cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt gtggaagtga
     2641 aagaagaact gccagttatc caaaaagaat atcagatcat agctactact gggattactg
     2701 atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa aatatcgaag
     2761 tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc atctccctag
     2821 atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc atctttatta
     2881 atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct cagacgacgg
     2941 gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca gcaggaatga
     3001 tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg acaaatacaa
     3061 cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa gatcagataa
     3121 aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat aatcagttag
     3181 ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta tagtgtatcg
     3241 tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa aaagttggca
     3301 cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa atgatagggg
     3361 tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt ggcacgtata
     3421 gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa
     3481 cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt tctactaaaa
     3541 gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat gattcaaatc
     3601 tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa tccccaagat
     3661 gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt
     3721 tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca agatattgat
     3781 tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt acctgctgac
     3841 aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca agaatggttg
     3901 ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt tttgaatttg
     3961 ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc aagtcatgga
     4021 caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca agaaaacgtc
     4081 aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa attggaagaa
     4141 gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt atggggcgga
     4201 acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg ggcaatcgtc
     4261 agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc aatggaatct
     4321 gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa agtaaaaccg
     4381 gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca acctaaagga
     4441 gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt
     4501 gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt gcaaccaaac
     4561 cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag actaatcgaa
     4621 caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat gatcgaagta
     4681 gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa tcctgaagac
     4741 gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg gtcaatggcg
     4801 cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa
     4861 gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat
     4921 tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa
     4981 taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata attttagtcg
     5041 tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa tttcatcaac
     5101 cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct ggtatcgtac
     5161 ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc gctgtagcac
     5221 cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt ggacaaggtg
     5281 ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca gggttgttct
     5341 taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat gcggctgctg
     5401 aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg
     5461 ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact gtgcaatcat
     5521 tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg ggaatggtcg
     5581 tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta tggccattct
     5641 tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct ctaggtgctt
     5701 taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt tcatcaaata
     5761 atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac tattaatctt
     5821 gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc
     5881 tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg
     5941 tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa aagaagaacg
     6001 ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca ttgcttctcc
     6061 aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga
     6121 tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg ttggtgaccc
     6181 agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat tagctatggg
     6241 aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc gttgggcatt
     6301 catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg atgacctttc
     6361 tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt tcgtattagc
     6421 tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt
     6481 agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag gaatcaagag
     6541 tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa caacattaca
     6601 agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat tgttatgtat
     6661 gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat tcgtagtcgg
     6721 tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg ggcgttgtcc
     6781 cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag aaaatggttc
     6841 aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg ggtctgacag
     6901 attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg cgagatagac
     6961 gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca gtcatgctaa
     7021 agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca tacacatttt
     7081 cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat gaagatagaa
     7141 tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac
     7201 gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt tactttgcag
     7261 aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat atgctaaaat
     7321 tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt gttggagccg
     7381 tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga
     7441 atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc gaatggggac
     7501 caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt ttggtagcag
     7561 ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa actaaaagga
     7621 gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa agaagttttc
     7681 cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt
     7741 gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc
     7801 aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt tacttaacga
     7861 actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt tttattactg
     7921 attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg
     7981 tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg cactcattag
     8041 cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa atcatcgatg
     8101 cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa gatgaagaag
     8161 aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat cacatcctga
     8221 aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac ttttatatgc
     8281 gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg
     8341 ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg
     8401 actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc gctattggtg
     8461 atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa ttagcagtta
     8521 ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct agagattata
     8581 tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg atcggtttag
     8641 agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg
     8701 tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt attgaggatg
     8761 acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca
     8821 tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca gactttgtta
     8881 tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt aagagataat
     8941 aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc agttttctta
     9001 ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct taggcaaagg
     9061 gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc aagatttagg
     9121 agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag cttctaaagt
     9181 agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat tattaggacg
     9241 tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga aagtagatct
     9301 ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg
     9361 ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca atatccatgt
     9421 agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt ttgaagacga
     9481 agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg gcattgcagg
     9541 ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa
     9601 tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt cctgtttatc
     9661 ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa gtgattgaaa
     9721 aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg gtgatcttgc
     9781 gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt gctttaaatg
     9841 aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc gtcaacggtg
     9901 ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct tttttctatg
     9961 aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa
    10021 tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct gaaaaaatgc
    10081 aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat cccggattag
    10141 tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat aaagaacgtg
    10201 tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg atttctgaag
    10261 acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat ggtatattgg
    10321 tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct atccgccctg
    10381 aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg ggtaaaccgg
    10441 acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat tctatcgatc
    10501 cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa ccgatggctc
    10561 agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg
    10621 ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga cattttgtca
    10681 caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat cgtgatgcta
    10741 acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca ggctggatca
    10801 ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat caaaccgtaa
    10861 aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta actgtcacta
    10921 cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa cgcgtatatt
    10981 taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa
    11041 aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa aaattacgat
    11101 tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta tatgaagcgg
    11161 gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt gctttatatc
    11221 aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa
    11281 aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc ggagcggtta
    11341 tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg gttttgttcc
    11401 caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc cagagagtag
    11461 aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg attgaggatt
    11521 tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat acgccggtga
    11581 tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca gatgttcggg
    11641 ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa gagttgatgc
    11701 gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc cagcgcaaac
    11761 aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa agattgtatg
    11821 aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct acacagcaaa
    11881 tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa caagtggctc
    11941 cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa cgcttagaag
    12001 agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct gttcaatctc
    12061 ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg acgttaaatg
    12121 atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg atccattatc
    12181 aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa aaatatggaa
    12241 aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa
    12301 ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga attaagcaaa
    12361 cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat gacagaatca
    12421 aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa ctgacttttc
    12481 agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg agcatacaac
    12541 ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg
    12601 tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg ttcccgtcta
    12661 tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg atgatttgcc
    12721 tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag tattcggcga
    12781 agcattagct atattggccg gagatggttt gcttacagga gcattccagc taatcagtat
    12841 ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg tttgtgcggg
    12901 atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga aaaagctttc
    12961 tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc gttatgcctt
    13021 gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt
    13081 agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg tgattggtac
    13141 aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa atacttaccc
    13201 gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt tatccgccaa
    13261 caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc tgatgcgcca
    13321 gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga acgcgtagat
    13381 gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg ttcagtgatg
    13441 gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga aaaaattcca
    13501 gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg agggggatta
    13561 aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac attgttagat
    13621 attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc aaaaatgagt
    13681 tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga tcctagagtc
    13741 gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac cttaggaatt
    13801 ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct gcctccatta
    13861 catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca attcgaagca
    13921 ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt
    13981 ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga attaagttat
    14041 tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag gaaagtaccc
    14101 gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa tgcacacgaa
    14161 cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg cggattcttc
    14221 tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa ggagcgaaga
    14281 cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg agcaagatat
    14341 ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc
    14401 tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt cagatggcgg
    14461 ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat tggaaaaact
    14521 attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt tgaaaacatt
    14581 gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga aagaattatt
    14641 ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag accaggcagt
    14701 tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt gatgctacaa
    14761 gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt tcacgaagga
    14821 atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga tgctttaggt
    14881 ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg
    14941 cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat ggttgaatta
    15001 gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt aactggaaaa
    15061 aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag aatcggcagt
    15121 tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc tgaatcacat
    15181 cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa gagttatcag
    15241 caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa
    15301 aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat agctgctgct
    15361 gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact tagcaattat
    15421 caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa tgaacaaaac
    15481 agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca tctagatgtg
    15541 gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca agatgcaatc
    15601 ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt agaagaagtt
    15661 acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc cattgagtca
    15721 atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt ttcagaaggt
    15781 caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca gcaacaagcc
    15841 gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat
    15901 gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt aaaagaagaa
    15961 aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac gaccaatcca
    16021 ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg
    16081 cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat ttttgatgaa
    16141 gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat ttctcaaata
    16201 tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc agtcgccgac
    16261 tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc tgtttcagag
    16321 ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag tgagattaca
    16381 aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac ctgagccacc
    16441 tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt acatgacact
    16501 cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc aaatgacaaa
    16561 ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt tcaacctcgt
    16621 cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag ggctttttta
    16681 taaaaacttc ccttttttta aataataggt agttacgata caaataaata atgttaatag
    16741 aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt tcattccata
    16801 gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta aaattttcat
    16861 aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt gttctactag
    16921 ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata catcacgtaa
    16981 atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt ctttcaatgc
    17041 atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga tatccatgat
    17101 ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag tatcgcattg
    17161 agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa tgaattgaaa
    17221 gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa agactttctt
    17281 taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg tcaaaataat
    17341 agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag caaaaggata
    17401 ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt ctgtacggga
    17461 attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa attgatgtgt
    17521 cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg tttggtcagt
    17581 ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga tcatgatttt
    17641 cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca tggaatgtgt
    17701 ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta
    17761 tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc acattcacac
    17821 cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc caatttcttg
    17881 taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg gcgattcctt
    17941 ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct atcttaccgt
    18001 ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa gcatattatt
    18061 atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt tttctgttga
    18121 caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta tgaaaaaaat
    18181 agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt
    18241 tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag tgggggattg
    18301 tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat gggtgaattc
    18361 caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg tgaagaactt
    18421 ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta tccgatgagc
    18481 gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa agacgcacgt
    18541 acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa acaaggacga
    18601 atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg cgtagtcatc
    18661 ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt ttctgcagca
    18721 gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact ataagaatag
    18781 gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg aaagaaacgg
    18841 tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc ggcggtgccg
    18901 gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat gcgtttgacc
    18961 aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta
    19021 tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt gagcatgtag
    19081 ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta gatgaagcag
    19141 aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat cagtcagctc
    19201 ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta aagattttct
    19261 tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa
    19321 aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa gtcatcccag
    19381 ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct gtgcggattg
    19441 cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc cggttattga
    19501 aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga attgtcaaaa
    19561 gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct gtcatacctg
    19621 acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct tcagaggaag
    19681 agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa aaaataagaa
    19741 tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga atatgattta
    19801 cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc
    19861 attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga aaaaattgct
    19921 gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat gctgcgcaca
    19981 aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag
    20041 caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga acggattaaa
    20101 caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa agtgaagtaa
    20161 atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa tccgatgaac
    20221 aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt ctttttattt
    20281 gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc attagctgaa
    20341 aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg aacgatttat
    20401 gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa agcgatactc
    20461 tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa
    20521 atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact gaatatttta
    20581 gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc
    20641 tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt
    20701 tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc tcattttatt
    20761 ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg tttggagtct
    20821 gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa agataatttt
    20881 caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc
    20941 tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga tcaagtaaat
    21001 gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac cggagaaatc
    21061 ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt gactggaaaa
    21121 gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc aaccatgaaa
    21181 gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga aaccttccaa
    21241 tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg tgaaaaaggt
    21301 gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt catactggaa
    21361 cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg acaaagtact
    21421 cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat tgtcgaccga
    21481 gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat gaaagctttc
    21541 acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa agtggtggat
    21601 ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc tttttcaaaa
    21661 gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga aaactacggt
    21721 agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc
    21781 caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt
    21841 gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct
    21901 aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg
    21961 gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta
    22021 gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg
    22081 caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat
    22141 cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac
    22201 gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt
    22261 agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact
    22321 gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat
    22381 ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat
    22441 gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg
    22501 accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat
    22561 cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct
    22621 tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat
    22681 aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat
    22741 tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa
    22801 tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct
    22861 tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt
    22921 aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt
    22981 attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa
    23041 acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc
    23101 aatttctata atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat
    23161 ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt
    23221 taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga
    23281 tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta
    23341 ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag
    23401 gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca
    23461 ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag
    23521 ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga
    23581 tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg
    23641 gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc ccaattattg
    23701 ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg
    23761 caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg
    23821 tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca
    23881 tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat gaagcacata
    23941 ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca
    24001 tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc
    24061 aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt
    24121 atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg
    24181 gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt
    24241 atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc
    24301 gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca
    24361 atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg
    24421 ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca
    24481 aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag
    24541 gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa
    24601 acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc
    24661 caaattttga aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa
    24721 agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat
    24781 atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc
    24841 atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct
    24901 ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg
    24961 gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca
    25021 gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa
    25081 atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt
    25141 ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct
    25201 gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa
    25261 tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc
    25321 ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt
    25381 ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat
    25441 aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag
    25501 gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc
    25561 acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag
    25621 actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa
    25681 ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa
    25741 aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat
    25801 gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag
    25861 aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg
    25921 ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca
    25981 taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc
    26041 aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa
    26101 acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc
    26161 acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt
    26221 ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata
    26281 agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt
    26341 ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa
    26401 tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat
    26461 ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa
    26521 cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca
    26581 aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac
    26641 aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat
    26701 tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga
    26761 aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg
    26821 gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag
    26881 aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa
    26941 cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt
    27001 caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa
    27061 attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta
    27121 gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt
    27181 cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta
    27241 tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag
    27301 ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat
    27361 cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa
    27421 agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga
    27481 aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt
    27541 cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc
    27601 aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat
    27661 gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca
    27721 taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc
    27781 tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat
    27841 tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac
    27901 caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac
    27961 gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac
    28021 atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga
    28081 tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa
    28141 agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg
    28201 agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa
    28261 attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat
    28321 cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg
    28381 tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag
    28441 ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa
    28501 tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg
    28561 aataagatat ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca
    28621 tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag
    28681 gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa
    28741 cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag
    28801 ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag
    28861 atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg
    28921 ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca
    28981 cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa
    29041 atgtggatac attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga
    29101 caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta
    29161 tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag
    29221 tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg
    29281 tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag
    29341 ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag
    29401 atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat
    29461 ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg
    29521 aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg
    29581 ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca
    29641 gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca
    29701 attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc
    29761 atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa
    29821 gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct
    29881 cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc
    29941 gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat
    30001 aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt
    30061 ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg
    30121 ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt
    30181 tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt
    30241 ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact
    30301 atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa
    30361 ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg
    30421 gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga
    30481 acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc
    30541 aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga
    30601 aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca
    30661 ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac
    30721 gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac
    30781 aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct
    30841 tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa
    30901 acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc
    30961 gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg
    31021 tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc
    31081 tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact
    31141 attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg
    31201 gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta
    31261 gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc
    31321 ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca
    31381 cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat
    31441 tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca
    31501 gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat
    31561 gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt
    31621 cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa
    31681 agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg
    31741 tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact
    31801 ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc
    31861 tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt
    31921 ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat
    31981 agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag
    32041 atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg
    32101 aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc
    32161 agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac
    32221 agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa
    32281 ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg
    32341 aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc
    32401 gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa
    32461 aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa
    32521 gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag
    32581 ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa
    32641 aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta
    32701 agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac
    32761 aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca
    32821 gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg
    32881 taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg
    32941 atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt
    33001 cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct
    33061 acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc
    33121 acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc
    33181 ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa
    33241 tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc
    33301 caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac
    33361 aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg
    33421 gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa
    33481 aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga
    33541 ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact
    33601 tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac
    33661 gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag
    33721 ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga
    33781 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
    33841 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
    33901 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
    33961 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
    34021 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
    34081 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
    34141 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
    34201 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
    34261 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
    34321 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
    34381 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
    34441 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
    34501 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
    34561 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
    34621 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
    34681 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
    34741 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
    34801 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
    34861 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
    34921 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
    34981 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
    35041 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
    35101 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
    35161 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
    35221 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
    35281 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
    35341 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
    35401 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
    35461 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
    35521 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
    35581 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
    35641 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
    35701 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
    35761 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
    35821 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
    35881 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
    35941 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
    36001 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
    36061 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
    36121 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
    36181 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
    36241 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
    36301 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
    36361 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
    36421 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
    36481 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
    36541 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
    36601 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
    36661 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
    36721 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
    36781 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
    36841 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
    36901 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
    36961 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
    37021 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
    37081 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
    37141 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
    37201 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
    37261 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
    37321 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
    37381 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
    37441 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
    37501 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
    37561 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
    37621 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
    37681 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
    37741 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
    37801 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
    37861 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
    37921 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
    37981 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
    38041 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
    38101 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
    38161 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
    38221 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
    38281 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
    38341 ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga
    38401 tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg ggagggatct
    38461 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
    38521 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
    38581 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
    38641 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
    38701 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
    38761 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
    38821 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
    38881 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
    38941 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
    39001 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
    39061 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
    39121 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
    39181 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
    39241 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
    39301 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
    39361 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
    39421 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
    39481 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
    39541 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
    39601 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
    39661 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
    39721 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
    39781 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
    39841 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
    39901 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
    39961 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
    40021 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
    40081 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
    40141 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
    40201 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
    40261 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
    40321 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
    40381 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
    40441 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
    40501 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
    40561 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
    40621 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
    40681 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
    40741 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
    40801 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
    40861 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
    40921 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
    40981 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
    41041 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
    41101 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
    41161 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
    41221 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
    41281 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
    41341 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
    41401 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
    41461 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
    41521 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
    41581 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
    41641 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
    41701 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
    41761 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
    41821 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
    41881 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
    41941 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
    42001 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
    42061 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
    42121 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
    42181 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
    42241 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
    42301 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
    42361 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
    42421 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
    42481 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
    42541 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
    42601 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
    42661 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
    42721 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
    42781 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
    42841 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
    42901 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
    42961 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
    43021 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
    43081 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
    43141 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
    43201 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
    43261 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
    43321 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
    43381 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
    43441 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
    43501 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
    43561 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
    43621 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
    43681 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
    43741 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
    43801 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
    43861 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
    43921 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
    43981 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
    44041 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
    44101 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
    44161 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
    44221 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
    44281 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
    44341 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
    44401 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
    44461 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
    44521 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
    44581 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
    44641 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
    44701 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
    44761 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
    44821 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
    44881 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
    44941 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
    45001 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
    45061 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
    45121 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
    45181 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
    45241 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
    45301 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
    45361 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
    45421 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
    45481 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
    45541 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
    45601 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
    45661 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
    45721 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
    45781 gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
    45841 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
    45901 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
    45961 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
    46021 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
    46081 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
    46141 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
    46201 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
    46261 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
    46321 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
    46381 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
    46441 atcattgatt tggtgcaaaa atatattgca gagccattcc taggattatc tcaagggttg
    46501 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
    46561 ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
    46621 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
    46681 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
    46741 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
    46801 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
    46861 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
    46921 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
    46981 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
    47041 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
    47101 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
    47161 gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat
    47221 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
    47281 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
    47341 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
    47401 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
    47461 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
    47521 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
    47581 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
    47641 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
    47701 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
    47761 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
    47821 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
    47881 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
    47941 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
    48001 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
    48061 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
    48121 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
    48181 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
    48241 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
    48301 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
    48361 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
    48421 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    48481 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    48541 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    48601 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    48661 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    48721 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    48781 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    48841 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    48901 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    48961 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    49021 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    49081 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    49141 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    49201 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    49261 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    49321 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    49381 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    49441 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    49501 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    49561 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    49621 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    49681 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    49741 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    49801 ataggtcgct ggttcaattc cagtcaggaa cgtacaaata acccctgtat cctttgcggt
    49861 acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    49921 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
    49981 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
    50041 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
    50101 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
    50161 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
    50221 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt
    50281 ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt
    50341 tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat ttcggaaaat
    50401 cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag atcctaccat
    50461 gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt catcggacat
    50521 ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt gattcgatgc
    50581 gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag gtgaagggtc
    50641 aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc
    50701 taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt taaaagtaaa
    50761 tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg aaatgtaacc
    50821 atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa
    50881 tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt taggcaatcc
    50941 gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt ctcgattcaa
    51001 ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga cagggtcttc
    51061 ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt gtttttcttc
    51121 tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca cgacagtcca
    51181 actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc cattcgctga
    51241 ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat cggcagaaac
    51301 aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga ttttagattg
    51361 tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt ggatctggtc
    51421 gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt gtgtgatcaa
    51481 tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt
    51541 ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat taaaatcaga
    51601 taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat gataccattt
    51661 aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt ttaaggttaa
    51721 gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca aaaactaatt
    51781 ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa
    51841 attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac agattttggc
    51901 agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga gaaacatcgc
    51961 gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata tggatgactt
    52021 gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt tcactgttga
    52081 gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg caagaagaga
    52141 gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag agatgcagaa
    52201 tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg tgaatctttc
    52261 agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg ggatcgcagc
    52321 tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga tgatccaaga
    52381 cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa tatactcaaa
    52441 agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct
    52501 tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg aataaaagag
    52561 ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa atttttgcga
    52621 ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca ttcagatcca
    52681 ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat
    52741 ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt
    52801 ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca tcaggcagat
    52861 cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg aattcatctg
    52921 catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca aatgcttctg
    52981 caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg atttttgcta
    53041 aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat accaaaacat
    53101 cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct aaatgtatgc
    53161 cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt actttaaaat
    53221 cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat gtacataaaa
    53281 atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta tttgtcagaa
    53341 actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg
    53401 cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa aagcctttga
    53461 aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc atttctgatt
    53521 cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg atggaagtaa
    53581 cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg attggtgaca
    53641 tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta
    53701 ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat tacggagaag
    53761 ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct atggttaata
    53821 ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca agagacggcg
    53881 gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca agaattttga
    53941 catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg ggagaggtac
    54001 atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat tgagacgact
    54061 caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc ttattattta
    54121 ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt tctccacatc
    54181 gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt ttaccagttt
    54241 atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt atccgtttct
    54301 gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat
    54361 aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt ctcactaatt
    54421 gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg gttgggacag
    54481 ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat cgatcagttc
    54541 caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt cttgatttat
    54601 ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac gatcttcgct
    54661 actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta ttttgcaacg
    54721 aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg gcttaatttt
    54781 gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga atacgtgacg
    54841 ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa attgaaaact
    54901 aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta tatcagtaat
    54961 ttataataat ta
//
LOCUS       DANJJW010000008        49271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000008 DANJJW010000000
VERSION     DANJJW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="PWS82_000473"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="PWS82_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL0905629.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="PWS82_000474"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="PWS82_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL0905630.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="PWS82_000475"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="PWS82_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL0905631.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="PWS82_000476"
     CDS             4735..5628
                     /locus_tag="PWS82_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905632.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="PWS82_000477"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="PWS82_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0905633.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="PWS82_000478"
     CDS             7565..8422
                     /locus_tag="PWS82_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL0905634.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="PWS82_000479"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="PWS82_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL0905635.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="PWS82_000480"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="PWS82_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL0905636.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="PWS82_000481"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="PWS82_000481"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0905637.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="PWS82_000482"
     CDS             11178..11936
                     /locus_tag="PWS82_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0905638.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="PWS82_000483"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="PWS82_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL0905639.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="PWS82_000484"
     CDS             12864..13895
                     /locus_tag="PWS82_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0905640.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="PWS82_000485"
     CDS             13892..14659
                     /locus_tag="PWS82_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0905641.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="PWS82_000486"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="PWS82_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL0905642.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="PWS82_000487"
     CDS             16624..16944
                     /locus_tag="PWS82_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL0905643.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="PWS82_000488"
     CDS             16944..17294
                     /locus_tag="PWS82_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL0905644.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="PWS82_000489"
     CDS             17461..18399
                     /locus_tag="PWS82_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL0905645.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="PWS82_000490"
     CDS             18414..19244
                     /locus_tag="PWS82_000490"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0905646.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="PWS82_000491"
     CDS             19288..20313
                     /locus_tag="PWS82_000491"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0905647.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="PWS82_000492"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="PWS82_000492"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0905648.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="PWS82_000493"
     CDS             21391..21831
                     /locus_tag="PWS82_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL0905649.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="PWS82_000494"
     CDS             21834..22436
                     /locus_tag="PWS82_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0905650.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="PWS82_000495"
     CDS             complement(22495..23601)
                     /locus_tag="PWS82_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0905651.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="PWS82_000496"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="PWS82_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL0905652.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="PWS82_000497"
     CDS             complement(25149..27494)
                     /locus_tag="PWS82_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0905653.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="PWS82_000498"
     CDS             27773..28675
                     /locus_tag="PWS82_000498"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL0905654.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="PWS82_000499"
     CDS             28888..30663
                     /locus_tag="PWS82_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL0905655.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="PWS82_000500"
     CDS             complement(30729..32315)
                     /locus_tag="PWS82_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL0905656.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="PWS82_000501"
     CDS             32566..32790
                     /locus_tag="PWS82_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL0905657.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="PWS82_000502"
     CDS             32932..34683
                     /locus_tag="PWS82_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905658.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="PWS82_000503"
     CDS             34683..36467
                     /locus_tag="PWS82_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905659.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="PWS82_000504"
     CDS             36793..37644
                     /locus_tag="PWS82_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0905660.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="PWS82_000505"
     CDS             37698..39449
                     /locus_tag="PWS82_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0905661.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="PWS82_000506"
     CDS             39453..39623
                     /locus_tag="PWS82_000506"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0905662.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="PWS82_000507"
     CDS             complement(39663..40565)
                     /locus_tag="PWS82_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0905663.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="PWS82_000508"
     CDS             40796..42199
                     /locus_tag="PWS82_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0905664.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="PWS82_000509"
     CDS             42199..43632
                     /locus_tag="PWS82_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0905665.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="PWS82_000510"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="PWS82_000510"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL0905666.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="PWS82_000511"
     CDS             44917..46644
                     /locus_tag="PWS82_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL0905667.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="PWS82_000512"
     CDS             46659..47009
                     /locus_tag="PWS82_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0905668.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="PWS82_000513"
     CDS             complement(47101..47961)
                     /locus_tag="PWS82_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL0905669.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="PWS82_000514"
     CDS             48053..48481
                     /locus_tag="PWS82_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0905670.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="PWS82_000515"
     CDS             48643..48819
                     /locus_tag="PWS82_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL0905671.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..>49271
                     /locus_tag="PWS82_000516"
     CDS             48846..>49271
                     /locus_tag="PWS82_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL0905672.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIV"
BASE COUNT        15966 a         8642 c        10573 g        14090 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt g
//
LOCUS       DANJJW010000009        45044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000009 DANJJW010000000
VERSION     DANJJW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            203..1642
                     /locus_tag="PWS82_000517"
     CDS             203..1642
                     /locus_tag="PWS82_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905673.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(1649..4201)
                     /locus_tag="PWS82_000518"
     CDS             complement(1649..4201)
                     /locus_tag="PWS82_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL0905674.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(4315..5646)
                     /locus_tag="PWS82_000519"
     CDS             complement(4315..5646)
                     /locus_tag="PWS82_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0905675.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(5612..7090)
                     /locus_tag="PWS82_000520"
     CDS             complement(5612..7090)
                     /locus_tag="PWS82_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0905676.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7395..9230)
                     /gene="tdc"
                     /locus_tag="PWS82_000521"
     CDS             complement(7395..9230)
                     /gene="tdc"
                     /locus_tag="PWS82_000521"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0905677.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            9700..11127
                     /locus_tag="PWS82_000522"
     CDS             9700..11127
                     /locus_tag="PWS82_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905678.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(11219..11584)
                     /locus_tag="PWS82_000523"
     CDS             complement(11219..11584)
                     /locus_tag="PWS82_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0905679.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(11581..11946)
                     /locus_tag="PWS82_000524"
     CDS             complement(11581..11946)
                     /locus_tag="PWS82_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0905680.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(12138..12386)
                     /locus_tag="PWS82_000525"
     CDS             complement(12138..12386)
                     /locus_tag="PWS82_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905681.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            12385..12513
                     /locus_tag="PWS82_000526"
     CDS             12385..12513
                     /locus_tag="PWS82_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL0905682.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12570..14264
                     /locus_tag="PWS82_000527"
     CDS             12570..14264
                     /locus_tag="PWS82_000527"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL0905683.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            14268..14387
                     /locus_tag="PWS82_000528"
                     /pseudo
     CDS             14268..14387
                     /locus_tag="PWS82_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            14364..14537
                     /locus_tag="PWS82_000529"
     CDS             14364..14537
                     /locus_tag="PWS82_000529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905684.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            14915..16048
                     /locus_tag="PWS82_000530"
     CDS             14915..16048
                     /locus_tag="PWS82_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL0905685.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            16079..16399
                     /locus_tag="PWS82_000531"
     CDS             16079..16399
                     /locus_tag="PWS82_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL0905686.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(16428..16892)
                     /locus_tag="PWS82_000532"
     CDS             complement(16428..16892)
                     /locus_tag="PWS82_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905687.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(17024..18277)
                     /locus_tag="PWS82_000533"
     CDS             complement(17024..18277)
                     /locus_tag="PWS82_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0905688.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(18306..18623)
                     /locus_tag="PWS82_000534"
     CDS             complement(18306..18623)
                     /locus_tag="PWS82_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0905689.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(18649..18969)
                     /locus_tag="PWS82_000535"
     CDS             complement(18649..18969)
                     /locus_tag="PWS82_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0905690.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(19433..20848)
                     /locus_tag="PWS82_000536"
     CDS             complement(19433..20848)
                     /locus_tag="PWS82_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0905691.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(20865..22181)
                     /locus_tag="PWS82_000537"
     CDS             complement(20865..22181)
                     /locus_tag="PWS82_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0905692.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(22331..23029)
                     /locus_tag="PWS82_000538"
     CDS             complement(22331..23029)
                     /locus_tag="PWS82_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL0905693.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(23318..24274)
                     /locus_tag="PWS82_000539"
     CDS             complement(23318..24274)
                     /locus_tag="PWS82_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0905694.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(24387..25220)
                     /gene="msrA"
                     /locus_tag="PWS82_000540"
     CDS             complement(24387..25220)
                     /gene="msrA"
                     /locus_tag="PWS82_000540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0905695.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(25395..27029)
                     /locus_tag="PWS82_000541"
     CDS             complement(25395..27029)
                     /locus_tag="PWS82_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905696.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(27016..28884)
                     /locus_tag="PWS82_000542"
     CDS             complement(27016..28884)
                     /locus_tag="PWS82_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905697.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            28850..30487
                     /locus_tag="PWS82_000543"
     CDS             28850..30487
                     /locus_tag="PWS82_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905698.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(30632..31372)
                     /gene="truA"
                     /locus_tag="PWS82_000544"
     CDS             complement(30632..31372)
                     /gene="truA"
                     /locus_tag="PWS82_000544"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0905699.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(31563..32198)
                     /locus_tag="PWS82_000545"
     CDS             complement(31563..32198)
                     /locus_tag="PWS82_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0905700.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(32345..33586)
                     /gene="sstT"
                     /locus_tag="PWS82_000546"
     CDS             complement(32345..33586)
                     /gene="sstT"
                     /locus_tag="PWS82_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL0905701.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            33784..34782
                     /locus_tag="PWS82_000547"
     CDS             33784..34782
                     /locus_tag="PWS82_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905702.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            34795..36813
                     /locus_tag="PWS82_000548"
     CDS             34795..36813
                     /locus_tag="PWS82_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL0905703.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(36873..37589)
                     /locus_tag="PWS82_000549"
     CDS             complement(36873..37589)
                     /locus_tag="PWS82_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL0905704.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(37816..38358)
                     /locus_tag="PWS82_000550"
     CDS             complement(37816..38358)
                     /locus_tag="PWS82_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0905705.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(38363..38590)
                     /locus_tag="PWS82_000551"
     CDS             complement(38363..38590)
                     /locus_tag="PWS82_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0905706.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(38669..40549)
                     /locus_tag="PWS82_000552"
     CDS             complement(38669..40549)
                     /locus_tag="PWS82_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0905707.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(40738..41352)
                     /locus_tag="PWS82_000553"
     CDS             complement(40738..41352)
                     /locus_tag="PWS82_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905708.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            41494..42054
                     /locus_tag="PWS82_000554"
     CDS             41494..42054
                     /locus_tag="PWS82_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0905709.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(42359..42673)
                     /locus_tag="PWS82_000555"
                     /pseudo
     CDS             complement(42359..42673)
                     /locus_tag="PWS82_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(42927..44562)
                     /locus_tag="PWS82_000556"
                     /pseudo
     CDS             complement(42927..44562)
                     /locus_tag="PWS82_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        14194 a         8895 c         7315 g        14640 t
ORIGIN      
        1 tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt ttttgatttt
       61 agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg ctccagaaaa
      121 ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct ccgtattctt
      181 agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga
      241 tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga taaccgtaaa
      301 tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc ttcaatctat
      361 tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa tcagtttgca
      421 aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac aatcagtcag
      481 tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg attttctagc
      541 cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca atcagcgttt
      601 agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc ttggaaaaga
      661 gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata acaactattt
      721 tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa acctcagtca
      781 aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct ttatcaatac
      841 gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca gcacactctc
      901 ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga
      961 cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca acaataacta
     1021 tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt
     1081 tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat ctctgactga
     1141 taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg aagcattgga
     1201 ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact tgtatcaaca
     1261 gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac caaattactt
     1321 tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac cgaatttgtt
     1381 caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc agcaggaatt
     1441 aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac
     1501 tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt ctctagatat
     1561 ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt tgactgaact
     1621 cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct gttttttctt
     1681 ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag aatagaagaa
     1741 tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct
     1801 gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa
     1861 gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac aactctctgc
     1921 aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg gtttgctgta
     1981 agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga
     2041 catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg
     2101 ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg
     2161 tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg cgattcagca
     2221 taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg
     2281 ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga
     2341 aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc
     2401 ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg
     2461 tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt ttatgttctg
     2521 gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca
     2581 taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca atttgttccg
     2641 caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt
     2701 gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga
     2761 tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt
     2821 ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac
     2881 tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact tctggtgcac
     2941 ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt
     3001 cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct gtttctgtgt
     3061 gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc tgttcttctt
     3121 ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag
     3181 ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca
     3241 ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg
     3301 ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat ccttcgggta
     3361 ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc ataccatatc
     3421 gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt
     3481 tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg
     3541 cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag
     3601 aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg atcgattctt
     3661 cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt
     3721 cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg
     3781 cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc
     3841 cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt
     3901 ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa
     3961 tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga
     4021 tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc tgctgccttg
     4081 cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc
     4141 cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca
     4201 tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt
     4261 atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct gggcctaact
     4321 gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa
     4381 taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa
     4441 aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat
     4501 aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat
     4561 gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc
     4621 ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag
     4681 cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa tcctgctagt
     4741 actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat
     4801 tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac tttcttttaa
     4861 atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa
     4921 gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt
     4981 ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa
     5041 tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc
     5101 tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat
     5161 aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt caccaaacca
     5221 cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa
     5281 agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta attccgcaag
     5341 tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc
     5401 taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc
     5461 acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa cgagaataac
     5521 agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc
     5581 gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt
     5641 ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag
     5701 gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga
     5761 ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg
     5821 ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc
     5881 caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc
     5941 ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg
     6001 cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact
     6061 gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg
     6121 gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag
     6181 cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat
     6241 gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca
     6301 tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata
     6361 taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt tccaatcgag
     6421 tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa
     6481 caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg
     6541 ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg
     6601 tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa
     6661 atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact aatgtaaatt
     6721 tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga gcgagtgctg
     6781 aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc
     6841 cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt tggggaaaca
     6901 ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca
     6961 gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg
     7021 ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag
     7081 tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt
     7141 tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa
     7201 acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta
     7261 tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata
     7321 caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt tattctttca
     7381 cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt
     7441 ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca
     7501 cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca
     7561 acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata
     7621 aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt
     7681 aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata
     7741 ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc
     7801 agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga
     7861 ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc ggcggcggtt
     7921 gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat
     7981 ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt
     8041 acgattcctc cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa
     8101 tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg
     8161 tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct
     8221 gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag
     8281 aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct
     8341 acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt
     8401 ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga
     8461 actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa
     8521 gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta
     8581 cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc
     8641 aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt cactttttca
     8701 ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta ccataatcct
     8761 tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat
     8821 aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc
     8881 aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc
     8941 aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct
     9001 gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga
     9061 taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt
     9121 tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca
     9181 ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc
     9241 tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac acctcttcac
     9301 tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag atttttacaa
     9361 acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg aacaattttt
     9421 caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa ataaaatgac
     9481 tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt cacaatttat
     9541 tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca
     9601 tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt
     9661 cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag
     9721 aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat
     9781 ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat
     9841 aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac
     9901 actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat
     9961 tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa
    10021 ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta
    10081 gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg
    10141 gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag
    10201 ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt
    10261 ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc
    10321 ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa
    10381 cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat
    10441 aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat
    10501 tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact
    10561 cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga
    10621 aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa
    10681 ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa
    10741 gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct
    10801 aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat
    10861 ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg
    10921 gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat
    10981 ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca
    11041 ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct
    11101 atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt
    11161 atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc
    11221 acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa atgatggttc
    11281 ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt
    11341 cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct
    11401 ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg acaaataaat
    11461 atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga
    11521 tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg
    11581 tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata
    11641 agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg
    11701 cttgataact gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata
    11761 acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt
    11821 gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc
    11881 gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg
    11941 aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag
    12001 ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt
    12061 tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta
    12121 gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct
    12181 ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta
    12241 cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct
    12301 ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa
    12361 agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta
    12421 ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac
    12481 agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga
    12541 catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg
    12601 ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg
    12661 atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca
    12721 ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg
    12781 ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc
    12841 gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg
    12901 caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg
    12961 gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc
    13021 ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc
    13081 cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac
    13141 gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa
    13201 tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc
    13261 ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg
    13321 actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta
    13381 ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt
    13441 tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag
    13501 aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca
    13561 acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta
    13621 ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac
    13681 caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag
    13741 atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg
    13801 aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac
    13861 agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag
    13921 tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa
    13981 ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg
    14041 atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg
    14101 agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg
    14161 ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag
    14221 atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa
    14281 aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga
    14341 tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg
    14401 aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt
    14461 tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
    14521 atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt
    14581 ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta
    14641 tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc
    14701 aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac
    14761 acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt
    14821 ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc
    14881 agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa
    14941 acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat
    15001 catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga
    15061 agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt
    15121 gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag
    15181 ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc
    15241 agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg
    15301 tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg taccagataa
    15361 tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag
    15421 cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt
    15481 cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga
    15541 tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt
    15601 agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt
    15661 cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt
    15721 agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa
    15781 atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca
    15841 agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat
    15901 gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca
    15961 ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa
    16021 aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat
    16081 gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat
    16141 acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt
    16201 cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc
    16261 cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat
    16321 tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag
    16381 caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta
    16441 tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc
    16501 cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca
    16561 agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc
    16621 ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag
    16681 tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac
    16741 cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca
    16801 gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt
    16861 tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg
    16921 tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa
    16981 aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt
    17041 cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa
    17101 gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc
    17161 caataattgg tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag
    17221 aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca
    17281 tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac
    17341 ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat
    17401 aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa
    17461 agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg
    17521 cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta
    17581 aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt
    17641 ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa
    17701 caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg
    17761 gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga
    17821 tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa
    17881 tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc
    17941 ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag
    18001 caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc
    18061 cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa
    18121 tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca
    18181 taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta
    18241 taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact
    18301 gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa atctgtaaaa
    18361 gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga
    18421 tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat
    18481 tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca
    18541 atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct
    18601 tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat
    18661 tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt
    18721 gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa
    18781 gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc
    18841 atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg
    18901 catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt
    18961 cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg
    19021 ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg
    19081 catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt
    19141 tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa
    19201 acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg
    19261 ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa
    19321 taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc
    19381 ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat
    19441 agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag
    19501 attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat
    19561 tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc
    19621 aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga
    19681 tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca tattctcttc
    19741 ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt agatacatgg
    19801 ctatatcata catgattttc ggatagattt cccatcccct ggaattattc attttcttcc
    19861 caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt
    19921 gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg
    19981 tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt
    20041 gacacaactc caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc
    20101 gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag
    20161 catagaccat ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt
    20221 aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt
    20281 ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga
    20341 ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt
    20401 gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca
    20461 aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga
    20521 tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact atacagttct
    20581 cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg
    20641 atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc
    20701 catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct
    20761 gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa
    20821 taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt
    20881 tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa
    20941 acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc attgacgatc
    21001 ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca
    21061 aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga
    21121 ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca
    21181 agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc
    21241 agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata
    21301 gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc
    21361 gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc
    21421 caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct
    21481 tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct
    21541 ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca
    21601 gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc
    21661 tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat
    21721 aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt
    21781 tcattcacta attcataaac agttggatta acagataaat aagacatcac tgcgatgata
    21841 ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca
    21901 gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat
    21961 tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt
    22021 gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg
    22081 ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca
    22141 ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga
    22201 cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact
    22261 cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc
    22321 agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta
    22381 ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct
    22441 gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt
    22501 tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc
    22561 cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc
    22621 cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct
    22681 tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa
    22741 ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg
    22801 caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc
    22861 tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg
    22921 acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc
    22981 taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct
    23041 ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt
    23101 ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca
    23161 taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa
    23221 aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt
    23281 ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc
    23341 tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact
    23401 gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg
    23461 caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga
    23521 ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg
    23581 ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg
    23641 gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta
    23701 gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc
    23761 tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat
    23821 ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg
    23881 tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga
    23941 gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt
    24001 cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat
    24061 cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat
    24121 tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat
    24181 tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt
    24241 tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa
    24301 tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta
    24361 gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc
    24421 catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta
    24481 taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta
    24541 ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc
    24601 tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga
    24661 tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa
    24721 tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct
    24781 tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg
    24841 cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata
    24901 caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca
    24961 tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag
    25021 tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt
    25081 tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat
    25141 acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg
    25201 aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt
    25261 agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct acagattttt
    25321 cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata
    25381 aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca
    25441 gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct tactttaata
    25501 tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt
    25561 acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt
    25621 acttggctct tctcattagt tactctatat atgatagaac taccagttgt tttataagga
    25681 aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc
    25741 aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc
    25801 tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa
    25861 ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat
    25921 agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa
    25981 gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta
    26041 ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca
    26101 taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa
    26161 gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc
    26221 agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt
    26281 ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc
    26341 attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct
    26401 ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat taatgttgat
    26461 agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga cggatacttt
    26521 tcttcttttc cagagatgat ttgataagca taaaataata cagggataac aagtgttatt
    26581 gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata agcagagaaa
    26641 atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa
    26701 tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca
    26761 atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa
    26821 aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa
    26881 atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata
    26941 ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc
    27001 aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata
    27061 tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca
    27121 ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat
    27181 ttctatctta ttttctccag catttacatc cacattcact aagccatgtt ttttataaga
    27241 gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac
    27301 tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt
    27361 atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct
    27421 ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata
    27481 acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc
    27541 gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat
    27601 taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga
    27661 agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt
    27721 tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc
    27781 aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa
    27841 cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat
    27901 gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat
    27961 ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt
    28021 caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt
    28081 gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt
    28141 taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc
    28201 tctattgaat atcaatccat aagcataata taaaacagct gggataatcg taagagcgat
    28261 tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata
    28321 taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa
    28381 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
    28441 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
    28501 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
    28561 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
    28621 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
    28681 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
    28741 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
    28801 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
    28861 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
    28921 tctattcatg tctaagggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
    28981 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
    29041 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
    29101 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
    29161 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
    29221 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
    29281 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
    29341 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
    29401 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
    29461 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
    29521 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
    29581 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
    29641 gacacatatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
    29701 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
    29761 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
    29821 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
    29881 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
    29941 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
    30001 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
    30061 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
    30121 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
    30181 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
    30241 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
    30301 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
    30361 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
    30421 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
    30481 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
    30541 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
    30601 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
    30661 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
    30721 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
    30781 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
    30841 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
    30901 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
    30961 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
    31021 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
    31081 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
    31141 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
    31201 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
    31261 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
    31321 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
    31381 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
    31441 agctccttgc ttctctaaga cagaagctat acaagaaaac tgtggaaata tatatttcag
    31501 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
    31561 ttttactgtt tgagatgcaa gatactcaac ctggcaatgg gcacataatt aaagaaaaaa
    31621 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
    31681 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
    31741 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
    31801 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
    31861 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
    31921 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtactaac aaatgcagca
    31981 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
    32041 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
    32101 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
    32161 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
    32221 cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa acctctattt
    32281 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
    32341 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
    32401 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
    32461 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
    32521 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
    32581 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
    32641 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
    32701 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
    32761 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
    32821 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
    32881 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
    32941 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg
    33001 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
    33061 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
    33121 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
    33181 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
    33241 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
    33301 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
    33361 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
    33421 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
    33481 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
    33541 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
    33601 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
    33661 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
    33721 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
    33781 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
    33841 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
    33901 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
    33961 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
    34021 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
    34081 ctaatagcca cactagtttg caatatcttt ttttacgtac aagctggcag cttgaatatt
    34141 cgtgattatt ggcttatcct aacacctatc agtcagaatt actttactta tgctgtttct
    34201 tgtactttgc ttttttgtag tattccttat acagttggat ttttaaataa attatcgaaa
    34261 acaacaatta aatatctact attattttta acaattggat tagttggctg ctctactcta
    34321 tttaataaag acccgtttgc attacagaat ggacagagta ttttatggat tttttattta
    34381 tttttaatgg gttatggttt gaaagaatgg aactggaaag aaaaggtccg ctataagctt
    34441 cctcaagtat ttatcagctt gattgtttta ttcggtttga ttattttgat gacacaaatt
    34501 tcactcatca tcagaggaaa tacagataca gcgctgaggt tttctaaacc atattctctc
    34561 ttcgcaatgt attatacttt ctcttcgttt ttatttttag aaatgctatc tgataaatta
    34621 ggtataaaag gaagaggaaa ttttcttgct gtatttttga ttgtcactca agttatatgt
    34681 aatttccctt tagtttctta tcgcgtatct acatttatga aacgtgaatt tccaaactca
    34741 gggaaggcat ggctgataaa tatcgggcag ttttttattt gatatttatt agcagtgtcc
    34801 ctcctagtta tctttttatt ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa
    34861 tacttcgctt ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag aattcattgg
    34921 ctttttgttc ataaaagtta ttttttagtt gcagcatttt tttactgctt taccttcgtg
    34981 cagattttcg ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa
    35041 atatttactc agcgacaagc acccatgatc ctgacaatca tcattattat gggatttttc
    35101 ttcttactat tgttgatttc caatcgtttt tggtacgcct tgactgctac cttgatcatt
    35161 aacttactgc tcactatttc gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt
    35221 ccttcagatt taaaaatgct gaccggattg tcagaactat tatcgatggt tagcccagtc
    35281 ttattaatcg tgggcggatt gatccttctc ctattattga ttactagtat tattgtacaa
    35341 cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc
    35401 gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct
    35461 ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta
    35521 aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa
    35581 cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca
    35641 gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta
    35701 agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt
    35761 ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat
    35821 ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg
    35881 tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat
    35941 attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc
    36001 agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat
    36061 aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac
    36121 aaagaaacct tgaaagcttt acaaagcaat acggaaacgt cacaattcat ccaattatcc
    36181 actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa
    36241 ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac
    36301 tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt
    36361 acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa
    36421 tatggattag aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa
    36481 caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt
    36541 gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat
    36601 ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag
    36661 gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa
    36721 gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt
    36781 gcaacatggg cgacacaaaa aataaaagac taacaaataa actgagcaag gggttgtgat
    36841 aaaagacatg tcacaacccc ttgtttatct cattaatttg cttgtccata aacgatttga
    36901 ttaaagccat ttttcaattc attattcgct gtctcgttga agtaaagata attatcggga
    36961 ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga
    37021 tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga
    37081 tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata
    37141 gccatcttcc actccccgtt caaaatagct tgagggactt taagaccata taagtccaca
    37201 ctcatttgat tcgtataact tttgtattgt tgtcccattg tttggcccca tttggtcata
    37261 aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcaac ttgttcgctg
    37321 ctttctgaag aggaagatga agcctctaca gaagaacttg aagtagatgt ttgagttgtt
    37381 tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttctgt acttgctgca
    37441 gttgacttgc tctctgtgct agttgttgta gtagttgcaa ctgttgcttt cgctgtttct
    37501 tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa aaaagctaga
    37561 ccaattgtta ctaaaatcct ttttttcaat taaaaaactc ctttcgatta ctcccacttc
    37621 tttattctat caaataagca atgtgatttt attgattttt cgtattttct catcaatctt
    37681 tcaaatcttc taaaaactga gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg
    37741 cttcttttat tttaatcttc gtgattttcc aatttacctc acgctttata tttgtatttg
    37801 aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt
    37861 tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa gttcaacaag
    37921 caattgttgc aagccatatt tttcttcttt ttcagctaat ttgattgccc gtgtgataaa
    37981 gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg ctggtaaata
    38041 tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta aagtagacgg
    38101 aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc tcgcttcttg
    38161 atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa
    38221 ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gctaatacat gccccaccat
    38281 ggcgccagca gttggtttat gatggtcgat atctgcttgt tttgtttctt gaacaaattt
    38341 ttcttccact atattcattg atttacgcct cttttacttt tgctgattgg acttcataac
    38401 ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga tcaaagtttg
    38461 ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc tgttgagaaa
    38521 ccgcatgttc gattttttgc atacagcttg gacaagctag tgtatctaat tgcataataa
    38581 tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag attacagctt
    38641 ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt tcgtgtccaa
    38701 aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg tgaacgaaca
    38761 tcccactagc catgtaaata aacccggcaa ataagccgtt gagtaaaaat aagacgacac
    38821 caatggcaat cactacattt tcacgggtat ttaaaacagt tttcttagcc aagcggtagg
    38881 catgaaccaa cgattcaaaa ttagattgca tcaagacaac gtcactcgtt tcgatagcaa
    38941 catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag ggagaatcat
    39001 tgattccatc tccaataaaa gcaacgcgca atccttgttc ttggtatttt ttcacaaatt
    39061 gaaccttttc gtctggtaat aattcagcat ggacttcatc aatgcctaac atatcagcta
    39121 cataatcagc agtcatctga ttatcacctg tcagcatcac taactgtttt gctcctgctt
    39181 gtttcaactt agctaattgt tcagcgactt ctggtcgaat cacatctgac acacctatga
    39241 tttgagtgat ttttttgtcg attgctacaa taatgaccga gctgcctgtt tgttgtaatt
    39301 gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca atcaatttgg
    39361 tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt cccttgattg
    39421 tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga gcataatgga
    39481 tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact aaagctaagc
    39541 tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt cctttggtca
    39601 atgtaccagt tttatcaaag acaaaagtat cgattttgga aaaagtatcc attgcttcac
    39661 cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca ttcgaaacag
    39721 gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatt gctaaacgaa
    39781 aatcacgact aatcagacca acaaccaacg caataagtaa aacggttgga gtataatact
    39841 gagaaaatcg atcaatgaat ttttccgcat gggatttggt atcttgtgct tcttctacta
    39901 attcgataat tttggcaaag gtcgtatcat ctccgacctt agttgcttca atcgtcacgt
    39961 agccatctgc taaaatcgtt ccagagaaga cttcatctcc actgattttg acgacttgtc
    40021 gtgattcacc agtcactgcg gcttcatccg catacccttt cccgtcaatg actcgtccgt
    40081 ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc acgtcatcga
    40141 tttccacttc ttcagtcgtt ccatcttctt ggacaacact agcagtagat ggggccatct
    40201 cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt tttttgttcc agatagctac
    40261 caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc ataaaggcac
    40321 caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttc actgcctgat
    40381 acgcatggat cataataggt atgaccgcaa taatagaagc tatggctaga gcaatgttat
    40441 agaacaagac aaaaccagta aattttccga taaaaccaaa aataatcaac acagctgata
    40501 aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc tttctccctc
    40561 ttttcctttc attcttacga gactaagtat agatacttat ccatcataaa aaattgacca
    40621 ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga atacactaca aaaaatagtt
    40681 agcaagaaga ctcactaaaa gtgatcttgc caactattgt cgtttctatc gaaattttca
    40741 ttgaatgatt ttcctgtaca gcatacatcg gagatctatt tctttatcta agatttttat
    40801 cttgctaatt ttttccttta gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca
    40861 gccactatct gtaaaaagga atatttcttt tcctttctct cttttttcaa attctgttag
    40921 caacttggta ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa aaatgatctc
    40981 tccatctggc gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta
    41041 agctccttta ctttctttag caaatatatt ttgtaaaaaa tcgaatatct taacatatat
    41101 ttgctgtcca aaagaacgat gtttcttttt ttctcctttt atctccgcca gcaaaacacc
    41161 tgccaactcg tcctctgcat acaaagcaag aatttgagtt gctcgattca actccatata
    41221 caaaaaatat cttccgtaaa gattcagcag aagttcattt tccatatatc tctcaaagtt
    41281 cattccttca acagcaaaat agatagcttt tttgtaatct ttcttttcta agggtttaaa
    41341 agttagtttc atcatttcgc ctcctcaact tcaatgattg tgttatactt acattataat
    41401 agacacagtg tctactatct ataatcaatc atctataagt ttgtcgatta agcgacagct
    41461 ttgctttgat atgtcggata aggagatagg taaatgaaaa agacagatag acgcgtcaaa
    41521 aaaacagaaa aagctttggc tgaaacacta tccacattat tagtaaataa aaagattcaa
    41581 gcaatcacta tacgcgaact aacagaaacg gcagatgtac atcgttcgac tttttataca
    41641 cattataagg atatctatga tctatatgat caattagaaa gcaacttttt tcgggatcta
    41701 aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac tcgcttgatt
    41761 gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca tggtgatatt
    41821 tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat atggctatat
    41881 gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta ccacgtccaa
    41941 ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc caaaaaagaa
    42001 ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc ttgaataaaa
    42061 acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg aatcttattt
    42121 agccaaattg tttactgaag attttttgag taaaatttct ataattccta cttattgaaa
    42181 agtagtttgt agtgctgctt ctccctctct aagtgatata tcttgttgta ccaatcaaat
    42241 tcacctcatc attaataaat tacttttagg ctaatttatt acaacttata ggttgaagct
    42301 gtaaccaatt tatagtcata ttatattttt atcatccagt ttattgaatt cttctcgttt
    42361 acctcaattc attttcaagg ggaatcagct catcatctaa cattttacgc catgctcgct
    42421 tcatttcttg tgggtattta aatggtttcg cttctggatg tttggcttta tatccttcgt
    42481 aatccttact gttgataatt gctttcatat taggaattgg atcttcacct ggcatatatt
    42541 gcagattaga agcatgtaat tttttctcag aaacaaacca ctcattggaa aagacatgaa
    42601 tcaaatcatc acttacttgt gtaaaatagc gacgtttaat gacaaagata tcttcattgc
    42661 tatctagtgt accttcattt ctttgtgctt aaattcaaag ataattttct caataaagat
    42721 ttttgattcc agtaagattc ctttactcct tttacaacat agcgttgtcc tttgttttga
    42781 aacatcttct tcccctcttt gccattaaaa atagcccaat tctctataaa gaagattaag
    42841 cttcattatt ataatataaa gtttaaattt ggtttattac ttactttttc ttattctcta
    42901 gtaagctaat attgaaaaaa atgtaatcag atcattattt ttatatgttg taacttcctg
    42961 ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa agctaacttc
    43021 atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt tccttcaatc
    43081 gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga ctgttcccca
    43141 ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg tatagactat
    43201 tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc ttacttcatg
    43261 attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc
    43321 gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt gatctctctg
    43381 agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca acagtaataa
    43441 aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct tcatgaaaac
    43501 gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc aaggaaattg
    43561 aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg tggataagag
    43621 ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa
    43681 gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg ttgtcaggtc
    43741 attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt gtaaggtcgt
    43801 tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat accaaaagta
    43861 tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca accggatgct
    43921 ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc caacggctat
    43981 tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac tgactgctaa
    44041 tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca taactatcgg
    44101 tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat caatcgtacg
    44161 gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc tttttaaaaa
    44221 ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc aaacatataa
    44281 gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg gaaaaaaggt
    44341 caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg taaaattgat
    44401 tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg caatatcccc
    44461 cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc cagcaataat
    44521 tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat tatatactcc
    44581 tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta tttattttta
    44641 ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg aacaccaaga
    44701 attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg tggctcttat
    44761 atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat aaaaaaaaca
    44821 gccatatccg ctttcttgga tatgactatt tactactact ttactatctt tcattcttct
    44881 tgtctcactt atgataaggc gctgcgtatc atgagtgaat gatattagga aaacttcata
    44941 aaataaatct tttatacatt tccacttcta gatggtcatt catgttagaa gaatcaaact
    45001 atttttttac tgtcgtaata atattaaaac actagaacaa atca
//
LOCUS       DANJJW010000010        43648 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000010 DANJJW010000000
VERSION     DANJJW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(97..441)
                     /locus_tag="PWS82_000557"
     CDS             complement(97..441)
                     /locus_tag="PWS82_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0905710.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(434..868)
                     /locus_tag="PWS82_000558"
     CDS             complement(434..868)
                     /locus_tag="PWS82_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL0905711.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1006..1743
                     /locus_tag="PWS82_000559"
     CDS             1006..1743
                     /locus_tag="PWS82_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905712.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1736..2947
                     /locus_tag="PWS82_000560"
     CDS             1736..2947
                     /locus_tag="PWS82_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905713.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3151..3933
                     /locus_tag="PWS82_000561"
     CDS             3151..3933
                     /locus_tag="PWS82_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL0905714.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4003..4665
                     /gene="trmB"
                     /locus_tag="PWS82_000562"
     CDS             4003..4665
                     /gene="trmB"
                     /locus_tag="PWS82_000562"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL0905715.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4763..6517)
                     /gene="ade"
                     /locus_tag="PWS82_000563"
     CDS             complement(4763..6517)
                     /gene="ade"
                     /locus_tag="PWS82_000563"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL0905716.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6649..7578
                     /gene="tadA"
                     /locus_tag="PWS82_000564"
     CDS             6649..7578
                     /gene="tadA"
                     /locus_tag="PWS82_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL0905717.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7535..8584
                     /locus_tag="PWS82_000565"
     CDS             7535..8584
                     /locus_tag="PWS82_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL0905718.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8581..8859
                     /locus_tag="PWS82_000566"
     CDS             8581..8859
                     /locus_tag="PWS82_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL0905719.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8834..9304
                     /locus_tag="PWS82_000567"
     CDS             8834..9304
                     /locus_tag="PWS82_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL0905720.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9321..9623
                     /locus_tag="PWS82_000568"
     CDS             9321..9623
                     /locus_tag="PWS82_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905721.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9640..10011
                     /locus_tag="PWS82_000569"
     CDS             9640..10011
                     /locus_tag="PWS82_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL0905722.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9983..10342
                     /locus_tag="PWS82_000570"
     CDS             9983..10342
                     /locus_tag="PWS82_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905723.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10514..11521
                     /locus_tag="PWS82_000571"
     CDS             10514..11521
                     /locus_tag="PWS82_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0905724.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11546..12730
                     /locus_tag="PWS82_000572"
     CDS             11546..12730
                     /locus_tag="PWS82_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL0905725.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13167..14750)
                     /locus_tag="PWS82_000573"
     CDS             complement(13167..14750)
                     /locus_tag="PWS82_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0905726.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15328..15471)
                     /locus_tag="PWS82_000574"
     CDS             complement(15328..15471)
                     /locus_tag="PWS82_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL0905727.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15693..16172)
                     /locus_tag="PWS82_000575"
     CDS             complement(15693..16172)
                     /locus_tag="PWS82_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0905728.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16245..16529)
                     /locus_tag="PWS82_000576"
     CDS             complement(16245..16529)
                     /locus_tag="PWS82_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905729.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16607..16801)
                     /gene="ssrS"
                     /locus_tag="PWS82_000577"
     ncRNA           complement(16607..16801)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWS82_000577"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16873..18159)
                     /locus_tag="PWS82_000578"
     CDS             complement(16873..18159)
                     /locus_tag="PWS82_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL0905730.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18297..18968
                     /locus_tag="PWS82_000579"
     CDS             18297..18968
                     /locus_tag="PWS82_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL0905731.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18934..19422
                     /locus_tag="PWS82_000580"
     CDS             18934..19422
                     /locus_tag="PWS82_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL0905732.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19435..20382
                     /gene="prmA"
                     /locus_tag="PWS82_000581"
     CDS             19435..20382
                     /gene="prmA"
                     /locus_tag="PWS82_000581"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL0905733.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20383..21147
                     /locus_tag="PWS82_000582"
     CDS             20383..21147
                     /locus_tag="PWS82_000582"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL0905734.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21380..23593
                     /locus_tag="PWS82_000583"
     CDS             21380..23593
                     /locus_tag="PWS82_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL0905735.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23604..24050
                     /locus_tag="PWS82_000584"
     CDS             23604..24050
                     /locus_tag="PWS82_000584"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL0905736.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24221..25480
                     /locus_tag="PWS82_000585"
     CDS             24221..25480
                     /locus_tag="PWS82_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL0905737.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25569..26516)
                     /locus_tag="PWS82_000586"
     CDS             complement(25569..26516)
                     /locus_tag="PWS82_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0905738.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26910..28202
                     /locus_tag="PWS82_000587"
     CDS             26910..28202
                     /locus_tag="PWS82_000587"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL0905739.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28217..29986
                     /gene="aspS"
                     /locus_tag="PWS82_000588"
     CDS             28217..29986
                     /gene="aspS"
                     /locus_tag="PWS82_000588"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL0905740.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30095..30970
                     /locus_tag="PWS82_000589"
     CDS             30095..30970
                     /locus_tag="PWS82_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0905741.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31236..33119
                     /locus_tag="PWS82_000590"
     CDS             31236..33119
                     /locus_tag="PWS82_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0905742.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33194..33478)
                     /locus_tag="PWS82_000591"
     CDS             complement(33194..33478)
                     /locus_tag="PWS82_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0905743.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33468..34202)
                     /locus_tag="PWS82_000592"
     CDS             complement(33468..34202)
                     /locus_tag="PWS82_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL0905744.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34331..34954
                     /locus_tag="PWS82_000593"
     CDS             34331..34954
                     /locus_tag="PWS82_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL0905745.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35146..36267
                     /locus_tag="PWS82_000594"
     CDS             35146..36267
                     /locus_tag="PWS82_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL0905746.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36274..39402
                     /locus_tag="PWS82_000595"
     CDS             36274..39402
                     /locus_tag="PWS82_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL0905747.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39421..42615
                     /locus_tag="PWS82_000596"
     CDS             39421..42615
                     /locus_tag="PWS82_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL0905748.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42761..43468)
                     /locus_tag="PWS82_000597"
     CDS             complement(42761..43468)
                     /locus_tag="PWS82_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0905749.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14084 a         7756 c         9216 g        12592 t
ORIGIN      
        1 tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa acttttctca
       61 cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag gatactctgg
      121 cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg gttcggcttg
      181 gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa tcacagattc
      241 agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt tatttaaatc
      301 atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg tttcattccc
      361 gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa ataacagtcc
      421 tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct tgaatagctt
      481 ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa aaatcatcct
      541 ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga taggctagtt
      601 cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt tccaatgctt
      661 gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca tattcaaaaa
      721 tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct tgagagatgt
      781 ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt tcttgattga
      841 tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag aataaatact
      901 tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa taccgtcatt
      961 ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag tttaattatc
     1021 gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag tttcgatgta
     1081 cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag tacgaccatc
     1141 aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa tcatctatcc
     1201 ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga gactccttct
     1261 ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc ctacgatata
     1321 cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg gttatctaat
     1381 cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt gatgatctta
     1441 tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt aggcttggat
     1501 ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga aggagccggt
     1561 attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg ttttgtggtc
     1621 ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa agaatttcag
     1681 ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca ggcacatgag
     1741 tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat atatgcgtta
     1801 tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg tgggattata
     1861 ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc tcataatctc
     1921 tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag aggcagcaga
     1981 tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac gagcttttcg
     2041 ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg ccatgccatt
     2101 agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga tcatattatg
     2161 gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac tagatcaaca
     2221 agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt tgtttctatc
     2281 attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa gtctatatta
     2341 ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg tgcatgtaga
     2401 agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg ttcctgaaat
     2461 tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata ttaaaagaga
     2521 atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt ctgattttag
     2581 tggtctttat ttccgattag taattatagg tgggttattg attgccggag tagtcgactt
     2641 ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc aactgttacc
     2701 tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc ataagaatca
     2761 gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt cggttatttt
     2821 tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc tagcggctta
     2881 tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga agaagatgtc
     2941 agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc gttgacagta
     3001 agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg caattaaatg
     3061 aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa taagtactgc
     3121 aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga ccaagagtgg
     3181 cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg tgatgaggac
     3241 aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag ggaaggaatt
     3301 acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac agcacaagag
     3361 tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga cgtgattgat
     3421 gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg tatcggtggt
     3481 cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc ggaagaactc
     3541 cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat cccgtcaata
     3601 gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg gttagtatgt
     3661 aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc tgcggtggat
     3721 attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg gctgatcagt
     3781 tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc ggccatgaat
     3841 ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat gaacgaagaa
     3901 attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct gcggagattt
     3961 ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt ccgaaatcgt
     4021 ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga tccaacattg
     4081 tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat tgagattggt
     4141 atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat caattatatt
     4201 ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat cgaacaaccg
     4261 cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta tttcgctgac
     4321 agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa acgtcatgaa
     4381 aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct tcgtccaaat
     4441 ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct ggcaagtttt
     4501 tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag tcaatttgaa
     4561 ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg tatttatcga
     4621 gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta actttctgct
     4681 ttcataaaaa cattaatatc cataaataaa tctcagaaaa tgaacattac agatgtaatg
     4741 tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt ccacaaggtg
     4801 gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc aattcgggta
     4861 tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta gctgctccta
     4921 gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa aaaggttcct
     4981 gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctgt ttgttgacaa
     5041 cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata tcctcgtcgt
     5101 ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct atcgctcctt
     5161 gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga tggtgacgct
     5221 cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt tcgccttgtc
     5281 ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt tcgatcacat
     5341 gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt tgagcgttta
     5401 gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg tcttggagtt
     5461 gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc agcttggtaa
     5521 gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct agtgttttca
     5581 aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat ggatcgattc
     5641 caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact aaatcattga
     5701 tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg atcgccggta
     5761 gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac ataccaagat
     5821 ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga atacctgctg
     5881 caggataaac atttaactct tccatgccga taccagctgc atgcccgtca atttttttct
     5941 tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt gctactgctt
     6001 gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa ggggctaatt
     6061 tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca caagaaggta
     6121 gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac tcaagacctg
     6181 tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta actccatgaa
     6241 gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata tgcacatggc
     6301 catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt tttccttcgt
     6361 attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc atgatttctt
     6421 tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc ttttttttac
     6481 ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc aagataaatt
     6541 tttataagta taacataaat tcggcttttg ccaatatttt tttgtaaatt cattttattt
     6601 gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat ggaaataaaa
     6661 cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga cgtatatata
     6721 ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg aacattctgg
     6781 aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta tttgggacag
     6841 atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc tttagaaaaa
     6901 caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga gagtttagtg
     6961 attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc tcgagatttt
     7021 gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg gacagtcgga
     7081 tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca agtgattact
     7141 atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat caatccgaaa
     7201 atagcacaga cttatgatac acttttgaaa ctcgcattac ggcatcgtcc agacttgcta
     7261 atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc agcactaaca
     7321 gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt ggtcagaatc
     7381 catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt catttatcaa
     7441 gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca atttatggag
     7501 gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga aaatatttgg
     7561 cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg cacaaaccat
     7621 gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat tactcacgat
     7681 ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg aagggcaagc
     7741 gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac agatcgagtt
     7801 agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac agtttcggct
     7861 tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc tattgctcac
     7921 ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc ttattggttc
     7981 aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc attggtatgt
     8041 tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc gtctgatgaa
     8101 gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg gaccgatata
     8161 ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc atgaaggttt
     8221 cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc tgattcagca
     8281 tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg agcagttcca
     8341 gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag aggcaaaggg
     8401 aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc ttttcaaacg
     8461 tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag cggctttgat
     8521 tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg aggttttgcc
     8581 atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat gctaatcgtc
     8641 ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg ttataggaat
     8701 catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca aaaagagctc
     8761 tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa tgaaggctat
     8821 atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt tagcatcagg
     8881 atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc tttcttttcc
     8941 agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt tcggccaatt
     9001 tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc aaacaggctt
     9061 ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag ataaaataaa
     9121 ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt ctattacttt
     9181 tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt gggaagaaaa
     9241 agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa aaagaattga
     9301 ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct tctgtctgtg
     9361 tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa agtggggagg
     9421 aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg tatcacaaaa
     9481 gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat aataatggag
     9541 taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa aaaacaagta
     9601 attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag cagtctattg
     9661 ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt gcagcgtaaa
     9721 gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa gagcagtcac
     9781 aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca gtatatcatc
     9841 gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca acctatgtta
     9901 acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt ttatgtacac
     9961 tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta gaaaatgtta
    10021 taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt ttttattgat
    10081 ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca aaaattatta
    10141 tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg ccgaaagttc
    10201 ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac aacagtccct
    10261 attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag taaaaaatga
    10321 tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt ctgttataac
    10381 actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga aaacgattga
    10441 aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa tatatctaga
    10501 aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa acctggaagc
    10561 tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg tcgaaaatgc
    10621 agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga caaaagagac
    10681 aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg agccagaaga
    10741 atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac atttgcaggc
    10801 gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta ttgaacaact
    10861 atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa tgggcaactt
    10921 gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag gaattggtgt
    10981 ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga cacaagcaaa
    11041 tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg tcgattttgc
    11101 aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag agtttttaac
    11161 aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat caatgaaata
    11221 tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag aaacggatca
    11281 aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa tgattcaatt
    11341 gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct tacagaaaaa
    11401 aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt cattaaaaga
    11461 accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat ctgctctgta
    11521 atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg ctgggagttc
    11581 aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga aagggatcgt
    11641 cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata atcaaaaatt
    11701 cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt tagatcagtt
    11761 gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc accgtgttgt
    11821 tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa tggaccgcat
    11881 tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg ggatcaaagc
    11941 gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat ctttccactc
    12001 aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg aagatttcaa
    12061 agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac gtgcggcaaa
    12121 attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag gaaatggtgt
    12181 atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat tcacaccatt
    12241 agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc tcccatattt
    12301 aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca agaaatctgg
    12361 tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga actatgaaaa
    12421 agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat acatcggcgg
    12481 ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta tcggcgagaa
    12541 tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt gtgaaatcga
    12601 tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag aatcaaaagt
    12661 aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg tggagcgtct
    12721 gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa gagggaattc
    12781 ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc aatttccctt
    12841 cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac
    12901 tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat tcatctttat
    12961 aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt gggctgtcga
    13021 ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat tgatcgaata
    13081 ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat
    13141 caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg
    13201 atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca
    13261 tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat ttcttctcga
    13321 gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat tttttctaaa
    13381 aagagtttca gcaacgccaa acggattttt tcaccaccgg ataaatcttt gatccgttta
    13441 cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac ttcttcgttt
    13501 ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc
    13561 tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc gtaaggcttt
    13621 tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc gccgatgatg
    13681 gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg
    13741 tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc accattagac
    13801 aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag tttcgccagg
    13861 cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc atgtgcccac
    13921 tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg cagttcgtaa
    13981 ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct gtagtttccg
    14041 ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga
    14101 aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa atagttttcc
    14161 aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa aagtaaatcg
    14221 gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc gctaagttct
    14281 gttaaccgtg cttcctgaca gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg
    14341 tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc gtaacgggcc
    14401 agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg actcatttgc
    14461 tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac ggtcgcttcg
    14521 gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg actgataaca
    14581 ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt gccagttccg
    14641 ttgatatcga ctaaggcaat tttttcacca gcttcaattt gacaggataa atcctcaaat
    14701 aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat gatctctctc
    14761 cttgagacca ctcaaaaagg ccatggttat ccctggcctg tgttatttaa actcagatta
    14821 tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact gttttggaca
    14881 aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg atatggtatc
    14941 gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt tccctccttc
    15001 gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt
    15061 tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg actgagggta
    15121 atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc gaaatcatat
    15181 ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt aataccgttg
    15241 cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag tatcgttgtg
    15301 gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga atgtagcact
    15361 tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa aatcggtatc
    15421 cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca cattgacgaa
    15481 ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact aatcgccctg
    15541 gtgttcactt tttttatttt acttgaactc attgactgtt ttccctttat tttaaacatt
    15601 ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa
    15661 aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc tttagctggc
    15721 aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt tctaataaca
    15781 tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt ggctcctaac
    15841 ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg ttttggtgag
    15901 ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt gctttcataa
    15961 tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc atcaaaggat
    16021 gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc tccgttattt
    16081 cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga accatctacg
    16141 gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag tcaatcgtat
    16201 tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg ctttggttca
    16261 gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac gatcagtaaa
    16321 aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat tacgcctaaa
    16381 acagcatatg cagcaccgta aaattgtagg aatccttgat ttttatcatt tttgttgatc
    16441 aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa taatactaaa
    16501 gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag caacaaatag
    16561 aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa tctccgttaa
    16621 tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt cccatggtcg
    16681 tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga cgggtcccaa
    16741 gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat cacagatttc
    16801 ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc agaaacagaa
    16861 ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac ggtaatactg
    16921 ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact gagcattctt
    16981 cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat gaggatactg
    17041 ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc tcaaatgatc
    17101 aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta atgccatgta
    17161 ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta gtgggatacg
    17221 tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac gagccgcagc
    17281 agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc ggcaaatact
    17341 tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat ccacatcact
    17401 tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc catttttgtc
    17461 atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa gatgaatcct
    17521 tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca atgcactccg
    17581 caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta actggattgc
    17641 ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca actggatgtc
    17701 ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat
    17761 ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac tttctaaatg
    17821 aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca ataagaggat
    17881 cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt ttgaatctgt
    17941 agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac ttgcgatact
    18001 cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc gagcatcgac
    18061 catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga cttcatcgat
    18121 cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct ccttcttgtt
    18181 tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag ccttctgttg
    18241 ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg agaaaaatga
    18301 cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa gtggcacggg
    18361 atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct ggatatattg
    18421 tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat ggtttaagaa
    18481 gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt tacacgatgc
    18541 atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa ggagtaatga
    18601 tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa
    18661 ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca attcctcaag
    18721 aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag aaaaaaaaga
    18781 caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt gtatggacag
    18841 aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa agaaaaaacc
    18901 aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct attaacatat
    18961 ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga ttgggataca
    19021 aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc acaatttgag
    19081 attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga agacggcgta
    19141 cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc agtaatccct
    19201 tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga atatctaaat
    19261 gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga cgatgacgaa
    19321 gctgtattcg agcttcactg ggataatcaa caactagcag aattagtaga agtaaaacag
    19381 caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac agatatgaaa
    19441 tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc taatattttg
    19501 atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga acattttcag
    19561 tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa agaaggcgca
    19621 ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa
    19681 gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa agtaacagtt
    19741 agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca tccggtgcgt
    19801 attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga agatcctcta
    19861 gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca tcccacgact
    19921 tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg
    19981 ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa agatgtctat
    20041 gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa
    20101 atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga gattgaaagt
    20161 gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc agatgcttgg
    20221 cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa
    20281 atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa
    20341 gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg gtatttttta
    20401 aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta tcaccacatt
    20461 gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa tgatcaaata
    20521 gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa agaaattaaa
    20581 aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg ctatcctaaa
    20641 ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca agcttttctt
    20701 ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa
    20761 caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca gatcgtgcca
    20821 tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga ttatgacgtg
    20881 gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt
    20941 cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga aggtggatta
    21001 tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg attaggacca
    21061 agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag ctatcaatgg
    21121 gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta tcgacagaaa
    21181 gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac aatggatacg
    21241 aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc
    21301 acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt accgaaaaaa
    21361 gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg gtgtcataaa
    21421 gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag catgcgaata
    21481 cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca ttattcatcc
    21541 tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag tggctacagg
    21601 cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga gagaagaatt
    21661 tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa
    21721 atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga
    21781 tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta cgttgaagca
    21841 cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct atgcgcctct
    21901 tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag cacttcgtta
    21961 tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga gagaagaacg
    22021 tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag agctggggat
    22081 ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta aaatgaaaga
    22141 tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga tcgtagattc
    22201 aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac caatgcccgg
    22261 cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt tgcatactac
    22321 cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg aaatgcatga
    22381 aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt
    22441 agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg aacttcaaga
    22501 cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct ttagtgataa
    22561 agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat ccggaccatt
    22621 ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg caaaagtaaa
    22681 tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg agatcatgac
    22741 ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc
    22801 aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg
    22861 ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag atattttgac
    22921 gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag atgatctcta
    22981 tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc tgactgaaaa
    23041 agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca
    23101 gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg
    23161 ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat
    23221 cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt gtccgaatgt
    23281 tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg aagatacatc
    23341 gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc gttcaggctt
    23401 attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc
    23461 acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc agaatttatc
    23521 ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca gtgtacgccg
    23581 gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt aaagctgctg
    23641 tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt ttattaggca
    23701 ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc
    23761 gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg ggaggacaaa
    23821 ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat cgaccaagct
    23881 ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc aatgaaggaa
    23941 tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg tcggtttctc
    24001 tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa agtaatctgg
    24061 ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa ctctaactta
    24121 agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc
    24181 ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag taccagtagt
    24241 agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga
    24301 gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat
    24361 cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa
    24421 aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt
    24481 ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag
    24541 aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca
    24601 tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg
    24661 ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca
    24721 aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgagaa
    24781 tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt
    24841 ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca
    24901 gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc
    24961 tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca
    25021 gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta
    25081 ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta
    25141 tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg
    25201 aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat
    25261 ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca
    25321 tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt
    25381 aaataagttt gagttctctg tacctgaaca agattggacg attccattca atcagtttcc
    25441 gacttatccg aatagccgag cgctacttta caagaattag attagcataa acttatcgaa
    25501 aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa attgataagg
    25561 tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa
    25621 tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata
    25681 actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct
    25741 tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt
    25801 tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca
    25861 ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg
    25921 attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg
    25981 tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac
    26041 gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta
    26101 gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg
    26161 taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa
    26221 ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt
    26281 gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt
    26341 ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact
    26401 tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct
    26461 cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct
    26521 gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt
    26581 gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt
    26641 aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc
    26701 gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga
    26761 aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg
    26821 tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat
    26881 ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg
    26941 atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat
    27001 tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat
    27061 cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca
    27121 aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg
    27181 ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac
    27241 caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag
    27301 tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg
    27361 atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata
    27421 aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag
    27481 agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta
    27541 aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg
    27601 agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt
    27661 atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa
    27721 tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg
    27781 acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta
    27841 tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt
    27901 tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc
    27961 aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag
    28021 cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg
    28081 agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc
    28141 cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt
    28201 aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca
    28261 attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt
    28321 aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat
    28381 caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac
    28441 tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt
    28501 cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat
    28561 tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg
    28621 tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca
    28681 ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc
    28741 agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt
    28801 gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta
    28861 tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac
    28921 gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg
    28981 attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg
    29041 tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga
    29101 tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat
    29161 ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta
    29221 ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct
    29281 tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga
    29341 agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg
    29401 cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa
    29461 agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca
    29521 atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc
    29581 aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta
    29641 tgatatcgtc ttgaacggtt atgaattagg cggaggttct atccgtattc atactcgtga
    29701 gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt
    29761 tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg
    29821 attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt
    29881 tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt
    29941 acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta
    30001 aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat
    30061 aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc
    30121 ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt
    30181 cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc
    30241 tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac
    30301 tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt
    30361 tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga
    30421 aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact
    30481 aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat
    30541 cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt
    30601 gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc
    30661 tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt
    30721 aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag
    30781 agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt
    30841 gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga
    30901 tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga
    30961 tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt
    31021 tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga
    31081 aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa
    31141 aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac
    31201 gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa
    31261 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
    31321 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
    31381 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
    31441 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
    31501 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
    31561 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
    31621 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
    31681 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
    31741 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
    31801 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
    31861 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
    31921 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
    31981 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
    32041 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
    32101 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
    32161 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
    32221 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
    32281 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
    32341 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
    32401 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
    32461 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
    32521 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
    32581 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
    32641 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
    32701 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
    32761 atttgttgaa agacactgtt ctgattggac taggaacgaa tggctcattc tcggaagctc
    32821 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
    32881 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
    32941 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
    33001 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
    33061 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
    33121 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
    33181 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
    33241 gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc
    33301 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
    33361 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
    33421 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
    33481 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
    33541 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
    33601 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
    33661 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
    33721 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
    33781 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
    33841 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
    33901 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
    33961 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
    34021 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
    34081 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
    34141 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
    34201 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
    34261 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
    34321 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
    34381 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
    34441 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
    34501 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
    34561 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
    34621 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
    34681 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
    34741 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
    34801 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
    34861 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
    34921 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
    34981 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
    35041 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
    35101 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
    35161 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
    35221 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
    35281 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
    35341 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
    35401 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
    35461 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
    35521 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
    35581 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
    35641 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
    35701 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
    35761 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
    35821 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
    35881 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
    35941 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
    36001 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
    36061 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
    36121 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
    36181 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
    36241 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
    36301 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
    36361 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
    36421 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
    36481 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
    36541 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
    36601 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
    36661 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
    36721 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
    36781 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
    36841 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
    36901 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
    36961 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
    37021 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
    37081 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
    37141 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
    37201 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
    37261 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
    37321 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
    37381 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
    37441 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
    37501 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
    37561 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
    37621 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
    37681 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
    37741 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
    37801 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
    37861 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
    37921 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
    37981 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
    38041 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
    38101 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
    38161 aagatggcca atgaaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
    38221 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
    38281 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
    38341 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
    38401 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
    38461 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
    38521 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
    38581 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
    38641 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
    38701 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
    38761 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
    38821 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
    38881 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
    38941 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
    39001 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
    39061 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
    39121 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
    39181 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
    39241 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
    39301 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
    39361 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
    39421 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
    39481 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
    39541 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
    39601 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
    39661 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
    39721 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
    39781 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
    39841 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
    39901 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
    39961 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
    40021 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
    40081 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
    40141 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
    40201 aacgatcaag gaatacaatt ttctgatcaa aacaaacctt tatcattcaa agtggaaaaa
    40261 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
    40321 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
    40381 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
    40441 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
    40501 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
    40561 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggtga agtcatctat
    40621 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
    40681 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
    40741 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
    40801 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
    40861 gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
    40921 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
    40981 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
    41041 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
    41101 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
    41161 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
    41221 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    41281 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    41341 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    41401 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    41461 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    41521 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    41581 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    41641 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    41701 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    41761 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    41821 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    41881 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    41941 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    42001 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    42061 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    42121 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    42181 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    42241 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    42301 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    42361 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    42421 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    42481 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    42541 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    42601 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    42661 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    42721 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    42781 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    42841 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    42901 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    42961 tctcagccag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    43021 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    43081 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    43141 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    43201 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    43261 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    43321 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    43381 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    43441 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    43501 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    43561 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    43621 tcactaaaaa tcgtgaaaca cgtcaata
//
LOCUS       DANJJW010000011        40254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000011 DANJJW010000000
VERSION     DANJJW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..755)
                     /locus_tag="PWS82_000598"
     CDS             complement(<1..755)
                     /locus_tag="PWS82_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0905750.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIE"
     gene            complement(766..1434)
                     /locus_tag="PWS82_000599"
     CDS             complement(766..1434)
                     /locus_tag="PWS82_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0905751.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1564..2148)
                     /locus_tag="PWS82_000600"
     CDS             complement(1564..2148)
                     /locus_tag="PWS82_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0905752.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2479..2712)
                     /locus_tag="PWS82_000601"
     CDS             complement(2479..2712)
                     /locus_tag="PWS82_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL0905753.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2727..3677)
                     /locus_tag="PWS82_000602"
     CDS             complement(2727..3677)
                     /locus_tag="PWS82_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0905754.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3674..4516)
                     /locus_tag="PWS82_000603"
     CDS             complement(3674..4516)
                     /locus_tag="PWS82_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0905755.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4518..5237)
                     /locus_tag="PWS82_000604"
     CDS             complement(4518..5237)
                     /locus_tag="PWS82_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0905756.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5929..6087
                     /locus_tag="PWS82_000605"
     CDS             5929..6087
                     /locus_tag="PWS82_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905757.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6159..7385
                     /locus_tag="PWS82_000606"
     CDS             6159..7385
                     /locus_tag="PWS82_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0905758.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7473..7865)
                     /gene="rpsI"
                     /locus_tag="PWS82_000607"
     CDS             complement(7473..7865)
                     /gene="rpsI"
                     /locus_tag="PWS82_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0905759.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7881..8324)
                     /gene="rplM"
                     /locus_tag="PWS82_000608"
     CDS             complement(7881..8324)
                     /gene="rplM"
                     /locus_tag="PWS82_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL0905760.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8843..9310)
                     /locus_tag="PWS82_000609"
     CDS             complement(8843..9310)
                     /locus_tag="PWS82_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL0905761.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9430..10968)
                     /locus_tag="PWS82_000610"
     CDS             complement(9430..10968)
                     /locus_tag="PWS82_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL0905762.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11048..11734
                     /locus_tag="PWS82_000611"
     CDS             11048..11734
                     /locus_tag="PWS82_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL0905763.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11935..12462)
                     /gene="msrA"
                     /locus_tag="PWS82_000612"
     CDS             complement(11935..12462)
                     /gene="msrA"
                     /locus_tag="PWS82_000612"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0905764.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12546..13223)
                     /gene="pgmB"
                     /locus_tag="PWS82_000613"
     CDS             complement(12546..13223)
                     /gene="pgmB"
                     /locus_tag="PWS82_000613"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0905765.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13220..14350)
                     /locus_tag="PWS82_000614"
     CDS             complement(13220..14350)
                     /locus_tag="PWS82_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0905766.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14350..16533)
                     /locus_tag="PWS82_000615"
     CDS             complement(14350..16533)
                     /locus_tag="PWS82_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0905767.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16530..17555)
                     /locus_tag="PWS82_000616"
     CDS             complement(16530..17555)
                     /locus_tag="PWS82_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0905768.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17600..18427)
                     /locus_tag="PWS82_000617"
     CDS             complement(17600..18427)
                     /locus_tag="PWS82_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0905769.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18424..19491)
                     /locus_tag="PWS82_000618"
     CDS             complement(18424..19491)
                     /locus_tag="PWS82_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL0905770.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19517..20353)
                     /locus_tag="PWS82_000619"
     CDS             complement(19517..20353)
                     /locus_tag="PWS82_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0905771.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20350..21219)
                     /locus_tag="PWS82_000620"
     CDS             complement(20350..21219)
                     /locus_tag="PWS82_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0905772.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21289..22572)
                     /locus_tag="PWS82_000621"
     CDS             complement(21289..22572)
                     /locus_tag="PWS82_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL0905773.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22586..24271)
                     /locus_tag="PWS82_000622"
     CDS             complement(22586..24271)
                     /locus_tag="PWS82_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL0905774.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24546..25571
                     /locus_tag="PWS82_000623"
     CDS             24546..25571
                     /locus_tag="PWS82_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0905775.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26166..29840)
                     /gene="rpoC"
                     /locus_tag="PWS82_000624"
     CDS             complement(26166..29840)
                     /gene="rpoC"
                     /locus_tag="PWS82_000624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL0905776.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(29898..33515)
                     /gene="rpoB"
                     /locus_tag="PWS82_000625"
     CDS             complement(29898..33515)
                     /gene="rpoB"
                     /locus_tag="PWS82_000625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0905777.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34243..35211
                     /locus_tag="PWS82_000626"
     CDS             34243..35211
                     /locus_tag="PWS82_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0905778.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35229..36134
                     /locus_tag="PWS82_000627"
     CDS             35229..36134
                     /locus_tag="PWS82_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905779.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36131..36937
                     /locus_tag="PWS82_000628"
     CDS             36131..36937
                     /locus_tag="PWS82_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905780.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37073..38062
                     /locus_tag="PWS82_000629"
     CDS             37073..38062
                     /locus_tag="PWS82_000629"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL0905781.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38379..38903)
                     /locus_tag="PWS82_000630"
     CDS             complement(38379..38903)
                     /locus_tag="PWS82_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL0905782.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39190..>40254
                     /gene="scm"
                     /locus_tag="PWS82_000631"
     CDS             39190..>40254
                     /gene="scm"
                     /locus_tag="PWS82_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0905783.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        11842 a         8320 c         7182 g        12910 t
ORIGIN      
        1 ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta
       61 ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat
      121 agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta
      181 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
      241 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
      301 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
      361 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
      421 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
      481 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
      541 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
      601 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
      661 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
      721 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
      781 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
      841 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
      901 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
      961 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
     1021 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
     1081 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
     1141 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
     1201 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
     1261 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
     1321 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
     1381 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
     1441 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
     1501 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
     1561 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
     1621 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
     1681 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
     1741 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
     1801 ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg
     1861 tttattctca aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact
     1921 tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat
     1981 attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt
     2041 aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt
     2101 agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg
     2161 agttataata taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt
     2221 attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt
     2281 agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt
     2341 aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat
     2401 tatatccagc tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct
     2461 ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc
     2521 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat
     2581 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
     2641 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt
     2701 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
     2761 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
     2821 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
     2881 tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
     2941 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
     3001 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
     3061 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
     3121 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
     3181 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
     3241 tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
     3301 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac
     3361 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa
     3421 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
     3481 atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa
     3541 ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt
     3601 attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
     3661 ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga
     3721 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
     3781 tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag
     3841 aaataaatat catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa
     3901 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
     3961 ttaatagaac catcaataga taatgataaa aactgacatt catccctatt gcttttgcca
     4021 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca
     4081 ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
     4141 caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
     4201 gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
     4261 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa
     4321 aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat
     4381 cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc
     4441 ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa
     4501 taaaattttg aagcatatta ttccccctct ccaataaata tagtatctcc ataggctttt
     4561 tttaaatttt cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc
     4621 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
     4681 actgtttttc cctgatgttt caattttttt aacgtatcca taattattcg ttcactaact
     4741 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
     4801 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
     4861 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
     4921 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
     4981 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
     5041 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
     5101 gctttaagca atgtggattt tccagcacca tttggaccaa taatacctgt taaagcacct
     5161 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
     5221 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
     5281 tatacaaaaa ttatatttta gtcaacccta aattttatag tttagatttt tttctgttgc
     5341 tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata
     5401 tataaaaaag gagcgttctc aaatgagaac atgctcctaa aactacttta ttggcgtatg
     5461 gaaaaattat acttgaagtg gatagcgggt tgaataagtt gataagcatc aaaacattta
     5521 taaaaattat tcaagactat gtctgttcca tatacaaata tgaaaatcag taaactccat
     5581 ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata gtgaatagga
     5641 aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga tccctgctat
     5701 tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta gagatttctt
     5761 tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac ttccgtaagg
     5821 ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca attcaaaaat
     5881 ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct gaataaaaaa
     5941 caaacctgtg catttctagg aatcgataat aatactctag ataagtggat tcgtatatat
     6001 aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga tattaaagac
     6061 tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac taaaattagt
     6121 gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg
     6181 ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat
     6241 cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata
     6301 tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag
     6361 gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc
     6421 aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat
     6481 ttcggttgca tattttacct atgtttggtg aatacgcaat gaattatcta aatatcaata
     6541 ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca
     6601 taaaaggata tgttcgttcc atgttcgata ttgcagaaat actaaattat atcgaattta
     6661 atcgaactac taaaattatt caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa
     6721 aaagaataca agaaggtaag caagcactat catcaaaaga acttactaat tggatagaag
     6781 cagtaaatga tgattttaat aatcatttac ttacttttca tgattacaca ctttttatga
     6841 ttaccctgta tcttggtgat agaaaaagtg agacttacgc tttgcaatgg aaatatattg
     6901 attttgaaaa acaaacggtc cgtcttaaac atactttaga taaatatcaa agaaaaaagt
     6961 ttacaaaagg tcgaaaagat accgtcattc aagtaccaga agttgtaatg acattgctaa
     7021 gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt aaaaattaag caaacgttag
     7081 atcagtatct ctttacttat actaaaccat ctggagaagt aaattgtcct gttcatgccg
     7141 attacctaaa ctatcgaatc aattcaatta aaagaaggca tcctgactta gttcatttat
     7201 ctccacacaa actccgtcat acttatgcaa ctatagctag acaaggtgga gcagatatga
     7261 atcaaatttc aaatgcttta acacactcag atatttctac aacaaagata tatgtgaaca
     7321 caccagatat agttgataag gctgtttttg aagcttttca gagaggattg aataagtgtg
     7381 attgatatgt gttcttaaat gtgttcttaa aaacaaaaga agcactttcc aaataattga
     7441 aaagtgccag aaacgttgat atataaagga tattaacgtt ttgagaattg tgaagcttta
     7501 cgtgctttct taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag
     7561 ccagcacgtt taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata
     7621 ccatgacgga tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg
     7681 tcatatgcgc cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca
     7741 gcatgtggaa tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt
     7801 actagacgta cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt
     7861 gccaatgaaa ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt
     7921 gctgcatgtg gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct
     7981 aaagtgtttt taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta
     8041 gcacgcaatt cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa
     8101 taaattttgt cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg
     8161 acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca
     8221 gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat
     8281 ttacgttcta cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat
     8341 ttaagttctg atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat
     8401 accatttaac atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa
     8461 aatttatatg aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa
     8521 aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa
     8581 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct
     8641 gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta
     8701 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     8761 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt
     8821 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
     8881 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
     8941 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
     9001 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
     9061 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
     9121 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
     9181 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
     9241 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
     9301 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
     9361 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
     9421 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
     9481 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
     9541 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
     9601 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
     9661 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
     9721 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
     9781 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     9841 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     9901 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     9961 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
    10021 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
    10081 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
    10141 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
    10201 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
    10261 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
    10321 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
    10381 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
    10441 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
    10501 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
    10561 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
    10621 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
    10681 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
    10741 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
    10801 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
    10861 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
    10921 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
    10981 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
    11041 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
    11101 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
    11161 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
    11221 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
    11281 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
    11341 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
    11401 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
    11461 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
    11521 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
    11581 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
    11641 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
    11701 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
    11761 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
    11821 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
    11881 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
    11941 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
    12001 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
    12061 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
    12121 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
    12181 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
    12241 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
    12301 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
    12361 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
    12421 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
    12481 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
    12541 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
    12601 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
    12661 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
    12721 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
    12781 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
    12841 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
    12901 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
    12961 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
    13021 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
    13081 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
    13141 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
    13201 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
    13261 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
    13321 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
    13381 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
    13441 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
    13501 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
    13561 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
    13621 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
    13681 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
    13741 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
    13801 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
    13861 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
    13921 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
    13981 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
    14041 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
    14101 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
    14161 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
    14221 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
    14281 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
    14341 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
    14401 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
    14461 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
    14521 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
    14581 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
    14641 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
    14701 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
    14761 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
    14821 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
    14881 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
    14941 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
    15001 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
    15061 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
    15121 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
    15181 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
    15241 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
    15301 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
    15361 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
    15421 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
    15481 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
    15541 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
    15601 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
    15661 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
    15721 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
    15781 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
    15841 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
    15901 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
    15961 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
    16021 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
    16081 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
    16141 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
    16201 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
    16261 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
    16321 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
    16381 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
    16441 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
    16501 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
    16561 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
    16621 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
    16681 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
    16741 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
    16801 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
    16861 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
    16921 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
    16981 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
    17041 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
    17101 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
    17161 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
    17221 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
    17281 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
    17341 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
    17401 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
    17461 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
    17521 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
    17581 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
    17641 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
    17701 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
    17761 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
    17821 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
    17881 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
    17941 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
    18001 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
    18061 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
    18121 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
    18181 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
    18241 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
    18301 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
    18361 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
    18421 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
    18481 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
    18541 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
    18601 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
    18661 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
    18721 ggcaacataa gaaatgatcc cgccatagcc gatccctatt aaagaagctt gtaatgcggc
    18781 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    18841 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt gtttttctgc
    18901 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    18961 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    19021 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    19081 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    19141 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    19201 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    19261 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    19321 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    19381 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    19441 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    19501 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    19561 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    19621 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    19681 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    19741 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    19801 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    19861 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    19921 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    19981 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    20041 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    20101 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    20161 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    20221 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    20281 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    20341 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    20401 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    20461 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    20521 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    20581 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    20641 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    20701 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    20761 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    20821 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    20881 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    20941 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    21001 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    21061 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    21121 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    21181 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    21241 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    21301 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    21361 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    21421 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    21481 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    21541 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    21601 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    21661 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    21721 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    21781 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    21841 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    21901 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    21961 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    22021 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    22081 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    22141 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    22201 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    22261 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    22321 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    22381 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    22441 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    22501 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    22561 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    22621 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    22681 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    22741 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    22801 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    22861 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    22921 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    22981 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    23041 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    23101 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    23161 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    23221 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    23281 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    23341 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    23401 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    23461 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    23521 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    23581 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    23641 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    23701 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    23761 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    23821 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    23881 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    23941 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    24001 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    24061 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    24121 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    24181 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    24241 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    24301 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    24361 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    24421 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    24481 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    24541 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    24601 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    24661 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    24721 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    24781 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    24841 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    24901 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    24961 aattggatca atataatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    25021 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    25081 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    25141 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    25201 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    25261 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    25321 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    25381 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    25441 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    25501 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    25561 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    25621 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    25681 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    25741 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    25801 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    25861 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    25921 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    25981 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    26041 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    26101 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    26161 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    26221 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    26281 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    26341 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    26401 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    26461 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    26521 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    26581 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    26641 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    26701 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    26761 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    26821 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    26881 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    26941 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    27001 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    27061 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    27121 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    27181 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    27241 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    27301 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    27361 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    27421 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    27481 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    27541 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    27601 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    27661 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    27721 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    27781 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    27841 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    27901 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    27961 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    28021 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    28081 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    28141 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    28201 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    28261 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    28321 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    28381 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    28441 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    28501 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    28561 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    28621 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    28681 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    28741 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    28801 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    28861 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    28921 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    28981 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    29041 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    29101 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    29161 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    29221 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    29281 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    29341 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    29401 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    29461 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    29521 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    29581 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    29641 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc acgttcaggt
    29701 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    29761 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    29821 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    29881 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    29941 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    30001 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    30061 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    30121 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    30181 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    30241 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    30301 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    30361 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    30421 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    30481 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    30541 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    30601 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    30661 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    30721 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    30781 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    30841 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    30901 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    30961 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    31021 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    31081 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    31141 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    31201 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    31261 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    31321 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    31381 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    31441 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    31501 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    31561 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    31621 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    31681 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    31741 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    31801 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    31861 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    31921 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    31981 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    32041 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    32101 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    32161 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    32221 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    32281 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    32341 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    32401 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    32461 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    32521 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    32581 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    32641 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    32701 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    32761 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    32821 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    32881 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    32941 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    33001 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    33061 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    33121 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    33181 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    33241 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    33301 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    33361 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    33421 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    33481 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    33541 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    33601 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    33661 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    33721 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    33781 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    33841 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    33901 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    33961 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    34021 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    34081 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    34141 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    34201 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    34261 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    34321 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    34381 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    34441 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    34501 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    34561 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    34621 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    34681 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    34741 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    34801 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    34861 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    34921 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    34981 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    35041 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    35101 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    35161 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    35221 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    35281 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    35341 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    35401 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    35461 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    35521 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    35581 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    35641 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    35701 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    35761 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    35821 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    35881 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    35941 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    36001 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    36061 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    36121 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    36181 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    36241 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    36301 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    36361 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    36421 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    36481 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    36541 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    36601 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    36661 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    36721 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    36781 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    36841 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    36901 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    36961 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    37021 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    37081 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    37141 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    37201 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    37261 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    37321 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    37381 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    37441 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    37501 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    37561 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    37621 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    37681 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    37741 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    37801 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    37861 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    37921 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    37981 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    38041 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    38101 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    38161 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    38221 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    38281 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    38341 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    38401 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    38461 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    38521 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    38581 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    38641 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    38701 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    38761 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    38821 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    38881 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    38941 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    39001 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    39061 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    39121 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    39181 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    39241 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    39301 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    39361 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    39421 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    39481 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    39541 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    39601 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    39661 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    39721 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    39781 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    39841 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    39901 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    39961 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    40021 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    40081 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    40141 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    40201 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DANJJW010000012        38903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000012 DANJJW010000000
VERSION     DANJJW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(59..187)
                     /locus_tag="PWS82_000632"
     CDS             complement(59..187)
                     /locus_tag="PWS82_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0905784.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWS82_000633"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWS82_000633"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0905785.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="PWS82_000634"
     CDS             complement(1674..2729)
                     /locus_tag="PWS82_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0905786.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWS82_000635"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWS82_000635"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL0905787.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWS82_000636"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWS82_000636"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL0905788.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWS82_000637"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWS82_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL0905789.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWS82_000638"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWS82_000638"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL0905790.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWS82_000639"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWS82_000639"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL0905791.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="PWS82_000640"
     CDS             10491..12050
                     /locus_tag="PWS82_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL0905792.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="PWS82_000641"
     CDS             complement(12161..14092)
                     /locus_tag="PWS82_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0905793.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWS82_000642"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWS82_000642"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0905794.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="PWS82_000643"
     CDS             complement(15021..15773)
                     /locus_tag="PWS82_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0905795.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="PWS82_000644"
     CDS             complement(15932..16141)
                     /locus_tag="PWS82_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905796.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="PWS82_000645"
     CDS             complement(16152..17480)
                     /locus_tag="PWS82_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905797.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="PWS82_000646"
     CDS             17616..17771
                     /locus_tag="PWS82_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905798.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="PWS82_000647"
     CDS             complement(17845..18432)
                     /locus_tag="PWS82_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL0905799.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWS82_000648"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWS82_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL0905800.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="PWS82_000649"
     CDS             complement(20012..21301)
                     /locus_tag="PWS82_000649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL0905801.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="PWS82_000650"
     CDS             complement(21455..22387)
                     /locus_tag="PWS82_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905802.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="PWS82_000651"
     CDS             complement(22544..23203)
                     /locus_tag="PWS82_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0905803.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWS82_000652"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWS82_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL0905804.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="PWS82_000653"
     CDS             complement(24244..24534)
                     /locus_tag="PWS82_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905805.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="PWS82_000654"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="PWS82_000654"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL0905806.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="PWS82_000655"
     CDS             complement(26820..28175)
                     /locus_tag="PWS82_000655"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL0905807.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="PWS82_000656"
     CDS             complement(28199..29368)
                     /locus_tag="PWS82_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905808.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="PWS82_000657"
     CDS             complement(29365..30252)
                     /locus_tag="PWS82_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL0905809.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWS82_000658"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWS82_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL0905810.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="PWS82_000659"
     CDS             34332..34853
                     /locus_tag="PWS82_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0905811.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35552..36109)
                     /locus_tag="PWS82_000660"
     CDS             complement(35552..36109)
                     /locus_tag="PWS82_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905812.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36288..36899)
                     /locus_tag="PWS82_000661"
     CDS             complement(36288..36899)
                     /locus_tag="PWS82_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905813.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37012..37269)
                     /locus_tag="PWS82_000662"
     CDS             complement(37012..37269)
                     /locus_tag="PWS82_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905814.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37866..38144)
                     /locus_tag="PWS82_000663"
     CDS             complement(37866..38144)
                     /locus_tag="PWS82_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905815.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38178..38429)
                     /locus_tag="PWS82_000664"
     CDS             complement(38178..38429)
                     /locus_tag="PWS82_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905816.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10628 a         8397 c         6824 g        13054 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
    34981 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
    35041 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
    35101 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
    35161 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
    35221 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
    35281 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
    35341 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
    35401 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
    35461 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
    35521 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
    35581 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
    35641 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
    35701 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
    35761 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
    35821 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
    35881 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
    35941 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
    36001 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
    36061 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat
    36121 cacctctaca ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg
    36181 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
    36241 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
    36301 ttgttaacaa tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa
    36361 acttcttgcg cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata
    36421 aagtctacct ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat
    36481 gacattgatt gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt
    36541 cctagatcgt ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt
    36601 cgttctcttt tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta
    36661 tcttcaatta tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca
    36721 taggtgattt ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag
    36781 cttggcaaca tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata
    36841 atttgtacaa ttctcattga tccattgtat tccattttat tccccctatt actattcata
    36901 tccgcctatc tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt
    36961 actgagttac ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac
    37021 atgattaatt ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc
    37081 gacaacataa gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa
    37141 atttactgtc gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc
    37201 ttcagcacca ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac
    37261 agccctcacc gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca
    37321 aaggatacaa tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa
    37381 taggcattaa ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat
    37441 ctcatacatt attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt
    37501 ttgtaattgg tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc
    37561 gtctatttgt gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac
    37621 tattttgctc caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta
    37681 attgatatgc tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct
    37741 tcttgggttt tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga
    37801 aatgccgttt ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac
    37861 tttgattaat ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa
    37921 tgttcgaact ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat
    37981 gttatcaaag ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt
    38041 ttctgcaggt aatctctcct aatggcagct atttttacct catcagtgct gataaaccat
    38101 tgatcatcca catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa
    38161 aagtttaagg ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg
    38221 agaattgaga acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg
    38281 tctctccatc cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa
    38341 aggaataatc cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat
    38401 gaccatttat ttataggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg
    38461 aatgaattca aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat
    38521 actttaaaaa agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt
    38581 agcaaataac ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag
    38641 ccaggataga cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag
    38701 ccttggaata cagtagctct taattggtaa tactttttcc attataaata acctcttttc
    38761 attttatatt tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc
    38821 ctcgctgtgc taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg
    38881 ccagaataaa ccaattattt gta
//
LOCUS       DANJJW010000013        37911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000013 DANJJW010000000
VERSION     DANJJW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(70..142)
                     /locus_tag="PWS82_000665"
     tRNA            complement(70..142)
                     /locus_tag="PWS82_000665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Thr,seq:ggt)
     gene            356..429
                     /locus_tag="PWS82_000666"
     tRNA            356..429
                     /locus_tag="PWS82_000666"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Asn,seq:gtt)
     gene            complement(567..731)
                     /locus_tag="PWS82_000667"
     CDS             complement(567..731)
                     /locus_tag="PWS82_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905817.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            1026..2354
                     /locus_tag="PWS82_000668"
     CDS             1026..2354
                     /locus_tag="PWS82_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL0905818.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2556..3389
                     /locus_tag="PWS82_000669"
     CDS             2556..3389
                     /locus_tag="PWS82_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL0905819.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3405..4142
                     /locus_tag="PWS82_000670"
     CDS             3405..4142
                     /locus_tag="PWS82_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL0905820.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(4297..5265)
                     /locus_tag="PWS82_000671"
     CDS             complement(4297..5265)
                     /locus_tag="PWS82_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL0905821.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5497..6330
                     /locus_tag="PWS82_000672"
     CDS             5497..6330
                     /locus_tag="PWS82_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL0905822.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            6364..7203
                     /locus_tag="PWS82_000673"
     CDS             6364..7203
                     /locus_tag="PWS82_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0905823.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            7229..7789
                     /locus_tag="PWS82_000674"
     CDS             7229..7789
                     /locus_tag="PWS82_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0905824.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7988..9709
                     /locus_tag="PWS82_000675"
     CDS             7988..9709
                     /locus_tag="PWS82_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL0905825.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9874..11016
                     /locus_tag="PWS82_000676"
     CDS             9874..11016
                     /locus_tag="PWS82_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0905826.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            11032..12243
                     /gene="thiI"
                     /locus_tag="PWS82_000677"
     CDS             11032..12243
                     /gene="thiI"
                     /locus_tag="PWS82_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL0905827.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12816..13463
                     /locus_tag="PWS82_000678"
     CDS             12816..13463
                     /locus_tag="PWS82_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0905828.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13592..13808
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13856..16501
                     /locus_tag="PWS82_000679"
     CDS             13856..16501
                     /locus_tag="PWS82_000679"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL0905829.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16811..18124
                     /locus_tag="PWS82_000680"
     CDS             16811..18124
                     /locus_tag="PWS82_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL0905830.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            18114..18767
                     /locus_tag="PWS82_000681"
     CDS             18114..18767
                     /locus_tag="PWS82_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0905831.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18822..19373
                     /gene="radC"
                     /locus_tag="PWS82_000682"
                     /pseudo
     CDS             18822..19373
                     /gene="radC"
                     /locus_tag="PWS82_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19504..20589
                     /locus_tag="PWS82_000683"
     CDS             19504..20589
                     /locus_tag="PWS82_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0905832.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20586..22478
                     /locus_tag="PWS82_000684"
     CDS             20586..22478
                     /locus_tag="PWS82_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0905833.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22485..22862
                     /locus_tag="PWS82_000685"
     CDS             22485..22862
                     /locus_tag="PWS82_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0905834.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            23016..23798
                     /locus_tag="PWS82_000686"
     CDS             23016..23798
                     /locus_tag="PWS82_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL0905835.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23924..24655)
                     /gene="erm(A)"
                     /locus_tag="PWS82_000687"
     CDS             complement(23924..24655)
                     /gene="erm(A)"
                     /locus_tag="PWS82_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL0905836.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24713..24772)
                     /locus_tag="PWS82_000688"
     CDS             complement(24713..24772)
                     /locus_tag="PWS82_000688"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL0905837.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            25164..25826
                     /locus_tag="PWS82_000689"
     CDS             25164..25826
                     /locus_tag="PWS82_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0905838.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            26061..26204
                     /locus_tag="PWS82_000690"
                     /pseudo
     CDS             26061..26204
                     /locus_tag="PWS82_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            26354..26554
                     /locus_tag="PWS82_000691"
     CDS             26354..26554
                     /locus_tag="PWS82_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0905839.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26808..28487)
                     /locus_tag="PWS82_000692"
     CDS             complement(26808..28487)
                     /locus_tag="PWS82_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0905840.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28489..28701)
                     /locus_tag="PWS82_000693"
     CDS             complement(28489..28701)
                     /locus_tag="PWS82_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0905841.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            29094..30824
                     /gene="efrA"
                     /locus_tag="PWS82_000694"
     CDS             29094..30824
                     /gene="efrA"
                     /locus_tag="PWS82_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL0905842.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30821..32581
                     /gene="efrB"
                     /locus_tag="PWS82_000695"
     CDS             30821..32581
                     /gene="efrB"
                     /locus_tag="PWS82_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL0905843.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32676..32873
                     /locus_tag="PWS82_000696"
     CDS             32676..32873
                     /locus_tag="PWS82_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905844.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            33026..33511
                     /locus_tag="PWS82_000697"
     CDS             33026..33511
                     /locus_tag="PWS82_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL0905845.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33615..34208)
                     /gene="clpP"
                     /locus_tag="PWS82_000698"
     CDS             complement(33615..34208)
                     /gene="clpP"
                     /locus_tag="PWS82_000698"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL0905846.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            34388..34915
                     /locus_tag="PWS82_000699"
     CDS             34388..34915
                     /locus_tag="PWS82_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905847.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            35006..35743
                     /locus_tag="PWS82_000700"
     CDS             35006..35743
                     /locus_tag="PWS82_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0905848.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35747..36664
                     /gene="miaA"
                     /locus_tag="PWS82_000701"
     CDS             35747..36664
                     /gene="miaA"
                     /locus_tag="PWS82_000701"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL0905849.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36666..37895
                     /gene="hflX"
                     /locus_tag="PWS82_000702"
     CDS             36666..37895
                     /gene="hflX"
                     /locus_tag="PWS82_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL0905850.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12734 a         6240 c         7920 g        11017 t
ORIGIN      
        1 ttcttacgtc ataaaataaa aaaacagcgc agacacattg ttaaaccaac atgttgcact
       61 gtttaaggaa gccacttgcc gggcttgaac cggcgacctc ttccttacca tggaagcgct
      121 ctaccagctg agctaaagcg gcattatcat taatacaaat aataggataa cgaatattaa
      181 tgtatttgtc aacagaattt tttcgaaaaa atgtgtttct ctaataggaa aacagatttt
      241 agaaaaaata aaaataacaa aaaatgcgaa cggtttcttt gataatataa taaaaaaatt
      301 caaaaaaagt attgcgttat ttagaagaaa ttgatattat attcatgtac ttgattccgg
      361 catagctcag ttggtagtag cgcatgactg ttaatcatga tgtcgtaggt tcgagtccta
      421 ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt
      481 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
      541 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      601 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      661 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      721 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      781 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      841 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      901 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      961 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
     1021 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
     1081 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
     1141 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
     1201 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
     1261 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
     1321 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
     1381 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
     1441 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1501 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1561 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1621 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1681 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1741 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1801 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1861 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1921 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1981 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     2041 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     2101 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     2161 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     2221 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     2281 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     2341 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     2401 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     2461 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2521 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2581 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2641 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2701 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2761 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2821 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2881 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2941 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     3001 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     3061 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     3121 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     3181 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     3241 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     3301 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     3361 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     3421 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3481 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3541 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3601 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3661 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3721 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3781 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3841 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3901 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3961 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     4021 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     4081 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     4141 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     4201 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     4261 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     4321 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     4381 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     4441 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4501 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4561 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4621 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4681 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4741 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4801 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4861 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4921 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4981 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     5041 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     5101 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     5161 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     5221 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     5281 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     5341 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     5401 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     5461 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5521 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5581 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5641 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5701 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5761 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5821 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5881 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5941 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     6001 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     6061 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     6121 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     6181 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     6241 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     6301 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     6361 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     6421 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6481 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6541 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6601 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6661 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6721 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6781 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6841 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6901 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6961 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     7021 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     7081 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     7141 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     7201 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     7261 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     7321 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     7381 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     7441 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7501 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7561 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7621 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7681 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7741 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7801 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7861 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7921 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7981 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     8041 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     8101 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     8161 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     8221 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     8281 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     8341 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     8401 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     8461 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8521 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8581 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8641 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8701 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8761 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8821 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8881 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8941 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     9001 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     9061 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     9121 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     9181 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     9241 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     9301 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     9361 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     9421 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9481 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9541 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9601 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9661 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9721 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9781 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9841 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9901 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9961 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
    10021 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
    10081 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
    10141 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
    10201 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
    10261 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
    10321 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
    10381 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
    10441 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10501 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10561 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10621 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10681 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10741 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10801 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10861 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10921 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10981 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    11041 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    11101 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    11161 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    11221 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    11281 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    11341 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    11401 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    11461 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11521 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11581 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11641 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11701 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11761 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11821 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11881 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11941 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    12001 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    12061 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    12121 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    12181 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    12241 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    12301 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    12361 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    12421 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12481 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12541 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12601 ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    12661 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
    12721 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
    12781 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
    12841 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
    12901 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
    12961 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
    13021 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
    13081 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
    13141 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
    13201 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
    13261 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
    13321 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
    13381 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
    13441 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
    13501 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
    13561 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
    13621 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
    13681 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
    13741 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
    13801 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
    13861 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
    13921 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
    13981 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
    14041 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
    14101 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
    14161 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
    14221 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
    14281 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
    14341 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
    14401 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
    14461 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
    14521 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
    14581 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
    14641 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
    14701 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
    14761 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
    14821 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
    14881 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
    14941 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
    15001 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
    15061 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
    15121 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
    15181 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
    15241 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
    15301 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
    15361 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
    15421 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
    15481 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
    15541 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
    15601 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
    15661 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
    15721 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
    15781 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
    15841 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
    15901 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
    15961 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
    16021 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
    16081 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
    16141 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
    16201 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
    16261 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
    16321 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
    16381 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
    16441 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
    16501 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
    16561 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
    16621 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
    16681 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
    16741 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
    16801 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
    16861 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
    16921 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
    16981 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
    17041 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
    17101 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
    17161 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
    17221 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
    17281 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
    17341 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
    17401 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
    17461 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
    17521 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
    17581 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
    17641 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
    17701 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
    17761 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
    17821 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
    17881 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
    17941 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
    18001 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
    18061 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
    18121 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
    18181 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
    18241 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
    18301 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
    18361 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
    18421 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
    18481 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
    18541 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
    18601 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
    18661 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
    18721 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
    18781 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
    18841 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
    18901 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
    18961 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
    19021 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
    19081 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
    19141 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
    19201 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
    19261 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
    19321 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt ttaaccatag
    19381 tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga tattttactt
    19441 taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa gaggtgggaa
    19501 catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta tttattacta
    19561 gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt agataatact
    19621 ggtaagtctc ctaataccat taaagcatac tgctatcatt taaagttgct gtacgagttc
    19681 atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc agacttcgta
    19741 ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa aaaagccata
    19801 agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct tgattattta
    19861 agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg aagaaatttc
    19921 aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt aaagttaagg
    19981 gttaaaaaga aacagataag aacattgaga tcagaggaag ttaagcaaat tattgatgct
    20041 tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg tttaagaatc
    20101 ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca aatccattta
    20161 acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga aagaacaata
    20221 catgtgagta aagaacttat gtcactttat acagattact tgatatatga gtatagtgag
    20281 gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg gaaaccacta
    20341 aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg aatagaattt
    20401 acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg atgggatgtt
    20461 gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa tacctatgtt
    20521 catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag aaaggagcat
    20581 aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt agcaaaaaaa
    20641 tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg gatataagaa
    20701 aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg aatcgttggg
    20761 ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat ttttattttt
    20821 accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa ggaacagtaa
    20881 ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca attactgaag
    20941 ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg ggtgttagaa
    21001 ttaccactac taattataag attactgcta atcaagaaaa aacacctgta aaagctaatt
    21061 cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt tattttgatg
    21121 gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca gatgataagg
    21181 ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat acatatttta
    21241 aacaacttgt aaaaagatat tgtaagttga gattgaacat ggatagcttt tcctatgtaa
    21301 gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa tttaaacaag
    21361 ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt gaactaaaca
    21421 tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa ggactattta
    21481 gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt tattctgagg
    21541 actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc ctagagcaat
    21601 tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg attgttcaag
    21661 aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta ttagaggaca
    21721 aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag catatagttc
    21781 caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt tcagaggaat
    21841 ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa caagaaacat
    21901 ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa tcaggtgaga
    21961 tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg attaacaacg
    22021 ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa atgacaagca
    22081 gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt caggaaaaac
    22141 tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc gatgatgtag
    22201 agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt tattgtagat
    22261 tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc actcacttct
    22321 gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca gaagagttat
    22381 tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc cgtgttaaag
    22441 agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat aaacaagtta
    22501 gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca agggaaaaag
    22561 tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt aattcaatag
    22621 caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt aggcaaagaa
    22681 tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc aagaaaagtt
    22741 ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa ttagaaaaag
    22801 aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat aataaagaat
    22861 aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg gtcagcttta
    22921 ttgaacagtc atttaggtat atgtccaatc tagggtaagt aaattgagta tcaatataaa
    22981 ctttatatga acataatcag ttacgaggtg aaatcatgag caatttgatt aacggaaaaa
    23041 taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga agttcaatag
    23101 ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac agcgatgtag
    23161 atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa ctaacagaaa
    23221 gactaatgac tatatcagga aagattggaa atacggattc tgttagacca cttgaagtta
    23281 cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga gaatttatat
    23341 acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca agctatgatc
    23401 ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta tttggtcctg
    23461 attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca attaaggatt
    23521 ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt ttaaccctag
    23581 ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc gctgcagaat
    23641 gggctatacc tcttttacct aaagagcatg taactttact ggatatagct agaaaaggct
    23701 atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa gcactcgtta
    23761 agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt gcaataacag
    23821 gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt gggaagaata
    23881 aagcgtcctc ttgtgaaatt agagaacgct ttattacttt aatttagtga aacaatttgt
    23941 aactattgaa aatagaaaga aattgttcct tcgatagttt attaatatta gtgacatttg
    24001 catgcttcaa agcctgtcgg aattggtttt tagtgaaaag aacacgatat tcacggttta
    24061 cccacttata aacaaaagat cgatactttt tgtagtcctt ctttgaaatc aatggttgat
    24121 gtcgttcaag aacaatcaat acagagtcta cacttggctt aggatgaaaa tatagtggtg
    24181 gtactttttt gagcattttt atatccatct ccaccattaa tagtaaaccc aaagctcgtt
    24241 gcagattttg caatcttttc gcaaatccct tctcaacgat aagatagcta tatttagcct
    24301 gactttcaaa ggtaattctt ttgacaatat ccgtactgat gttataagga atattaccat
    24361 atatcttata gtttatatgt tttgggaagg aaaattttag aatatccgtt tgaatcactt
    24421 ttatattctc agaggggttt accgcttctt tagtcacttg acataagcct ccatcaattt
    24481 ctatagcagt aactgatcga ctcattttga ctagctcttt ggtaaaatgt ccttttcctg
    24541 atccgatttc tattacgttg tcttgtttac tgatattcgt gtgattcaat atttctttta
    24601 catgcttttt agaagtaata aaattttgcg tgtctttagg gtttttctgg ttcattataa
    24661 ccttctcctt actggttata atgaactgac attaagcgtt cattataacc aattaatttt
    24721 gatttggttg ataacgaact ttattaataa caaatataga aaaagtaccc atacttttat
    24781 ctcctttcca tttttttcat tatgaatgag ataaagtaat ttctactact gcgatactgg
    24841 tgcacataat tgaaacctcc ttatgtaact gaatatgatt gtatcatctt ttcattttag
    24901 aataaacctt tatatgtaat tttgcacttg acacttcaga tggacgtttt cttcaaaata
    24961 atatgttttg aaacttgata agagaagcca ctgtctttac taaaaagtaa aggctgcgat
    25021 aaaggacagt gactttaata aagttgcact gttgtattgt ctttggataa aattgtctaa
    25081 agggaagttt tatccccaac gaaaaaacat ctaaagtggt aagtaatgaa agaatgtcga
    25141 taaagtagga atgggggatc tttttgaata caatagaatc acagtttgat aaggttgcag
    25201 aagattacga tttcgtgaat gagcttttga atgattattc attctttgtg tctaatatgt
    25261 ctccaaagaa aggcagagca ttagatatcg gatgtggctc gggtttgtta gtggagaaat
    25321 tagcaagtta ttatgatgaa gtggtaggga ttgatatttc taatcaaatg ctcgatcttg
    25381 ccaaatctaa acgtcaacta acaaatacgg tctatctgaa tatgaatgca gaacaactta
    25441 attttaatga gaagtttgat tttattgtaa gccgaacaac ctttcatcat ttggatgata
    25501 tagccagcgt aatacagcaa atgaaggaac tgttgaatga ggaaggaaga atagttattc
    25561 ttgataatgt atctgaagtc gagacaccgc ctacctatgt ttataaatta ggggcaatcc
    25621 aagagttttt accgcattgt ttcaaatttg gaataaaaaa tgcgattaga atttataatc
    25681 acaacacatc taaatcatgg cttgagcatt tagcttcaga taagtaccta tctgaacaaa
    25741 attattacga tttatatgaa aagttgttgc ctggatgcca attccataaa atgggttggg
    25801 caatgggggt tgtctggaca aaataactta aagctgtggc tttgaaaagt tgaatttgtg
    25861 attgttttca gagctatcgg gtgctttagt ataaataaca atgaataaaa aatcagcctg
    25921 tatcttatgt tactattaag taatttaaga tacaggtttt actatttttg aaataagtac
    25981 cttccacttc aaatacatgt attcaaagtg aaagtattga tttaaatgat tcttttgtaa
    26041 tttactcttg ataatatgaa attccatcgc agcaggatat gaattttacc aaaaggatac
    26101 aaaagtgtgg agaaatgatg gggatcactg tactggacca cttgatcatc ggacgcaaac
    26161 gatattttag tttgagagaa gaaggaatga tggaagagaa gtaagttgct ttcttgcaaa
    26221 aaaaatgaag caagtgtaca attatattgt aattttgttt gggaatagaa gttgaaacat
    26281 ctgttgcacc tctatcgtac acgagcaatg ttacaattta aattagtgct tttggcacga
    26341 ggaggatatt attatggaaa acggaacagt aaaatggttc aacgctgaaa aaggatttgg
    26401 atttatctca cgtgaagatg gaagcgacgt attcgttcac ttctcagcta tccaaggtga
    26461 cggattcaaa acattagaag aaggtcaagc agtgactttc gacgttgaag aatcagaccg
    26521 cggcccacaa gctgcaaacg ttgtaaaagc ttaattttat ctgtaaatca aattgatggt
    26581 agacatcaat ttttacgaga gaacaaagcg agtaacaaaa atacctgcca caagagacaa
    26641 tttctcttgt ggcaggtatt tttttgtatg aaaaagtaaa taaatttaag aggctatgaa
    26701 agagccacct tgtatcaagg aataagaaca aacaaaaatg gcttctcatg tttttcattt
    26761 gttttctctt atattcagga caaggtgctg atttcataac ctcttattta ttcttcatct
    26821 ggtgtcatta ccattggcaa aatcattgga tgtctttctg tacgttcgaa taagaaaggc
    26881 tgaagcgcgc cagtcatcgc ttctttcaat ttatattctg aacagtcagg tccattcaaa
    26941 cattctctta aagcgtggaa taagattcgc tgagcttcat tgatcatttc tcctgattct
    27001 cgcatgtaga caaatccacg agagagaata tctggaccag ccaagatttc ttttttcttg
    27061 atatccactg tcgcgacagc taatacaaga ccttcttctg aaagcaggtg acggtcacgt
    27121 aaaacgacat taccaatatc accgatcccg ctaccatcga tatacacatc tccagccaca
    27181 aaatgcccag caggacgcgc agaatcgcta gttaacgcta aaacatcgcc attttccatg
    27241 atgaagcagt tttctgcagg aacacctaca tcttgtgcta gagaagcatg tactttcaac
    27301 atgcggaatt ctccgtggac aggaataaaa tattttggct tcatcaaacg aagcattaat
    27361 ttttgttctt cttgtccacc gtgaccggaa gtatggatgt tattgatttt tccgtgaatc
    27421 acttctgcac cagcttcaga cagcaaattg atcaaatgat tgacacttgt tgtattacca
    27481 ggaataggtg aacttgaaaa gatcactgta tcgtctggct ggatttggat ttgacggtga
    27541 gtaccattcg cgatgcggct caacgcggcc atcgcttctc cttgagaacc tgtacatagg
    27601 atcatcgttt cattggcagg caagcgattg atttcgtgtg catctacaaa tgtgccgtct
    27661 ggaactttga tatatcctaa acgttgaccg ttgacgatgg cattttccat gcttcgtccg
    27721 aaaacagcga ttttacgacc agttgccaca gcggcatctg ctgcttgctg caaacggaag
    27781 atgttagaag caaagctggc aaaaatgata cgtccttcga tttttgaaaa gatcttcaaa
    27841 atagaatgcc caatcgtttt ttctgattta gtaaatgttg gaatctcagc atttgtacta
    27901 tcagataaaa ggcacaagac tccttcttcc ccgatacgag ccattttatg caagtttgct
    27961 ggttcgccaa caggagtgaa atcgaatttg aagtctccag tcgctacgat atttccagaa
    28021 ggtgttttca ccacgatccc taatgcatct gggatactat gggtcgttct gaaaaagctg
    28081 acagaagtct ttctaaaacg gatgatcgta tcttcattga tctcatggag ctctgcatct
    28141 cgaagcaagc catgctcatc taatttgttt gtgatcaacg ctaatgcgag aggaccagca
    28201 tagataggaa tatttgcttg tttcagcaag tagggtattc cgccgatgtg gtcttcgtgc
    28261 ccgtgagtaa tcaccaaggc ttttactttg tgtaagtttt gtatgatgta gctgtagtcg
    28321 ggaatgacat agtcaattcc aagtaaatcg tcttccggga atttgatccc ggcatcaatc
    28381 aaaatgattt catcttggaa ttgtacccca tacgtatttt tcccgatttc acctaatcca
    28441 ccaatagcaa agacgcctgt ctcgttattt ttgatggaaa gtttcatatt aaaactccac
    28501 taatgagaaa tcagcatttt ctttttcgta ctctagatga cttccttcta gaagttgaac
    28561 gaattcaata ttgtatgggg tattttcttc cacacgttgg cgagcgtcta catcgctttt
    28621 tgcctcaatg taaagagatt ttgtttcttc ccgtttaggg ttgcgtactt ttgtttcttg
    28681 ataataaact ttatagatca ttgttttgtc tcctttatct gcgtaaagca gtgatttagc
    28741 tagttgattt ttttagacaa cacaaaacga ggggacaagc cacctggttt ttcatatgct
    28801 ttgactagaa tactggattc gagtcaaaga gacaagcctc gtttttgctt atctgttcgg
    28861 atattttttc aaccgtacct tctgtataaa aaatatacaa caattaattg ttattctagc
    28921 ataaaacagc aaataagtat agaaatcaac tagattgaaa gaaaaaatca atgtttatca
    28981 tcccgctata atcataatga cttttttatg agagaagtgt tctattaata ggctcgttgt
    29041 gctatcattt ttagtaatga aaatgtaaca aaagattaaa aggagatgag aaagtgaaac
    29101 taatgtggca ttataccatg cgttataaga aattcttgtt tttcaatttc atatgcgtat
    29161 ttggttttat tttgattgaa ttaggacttc ctacgatttt agcacgtatg atcgatgtag
    29221 gaatccgcaa tgatgatttt gattatgtga agcaacaagg gatatttatg atcgttatta
    29281 ctgtaatcgg tattattttg aatatcttgt taggttattt tggttcgagg attacgacta
    29341 atatcgtagc agatattcga gatgatttgt tcgaaaagat ccaaagttat tcgcatcagg
    29401 aatacgaaaa tctaggcgta tcttcattga tcactcgtac aacaaacgat gcttatcaga
    29461 tcatgttgtt cctgcaaaat attttacgta tcggttttat ggcaccaatg atgttcattg
    29521 tcagtttgta catggtcatg cgtacaagtg tgacactcag tttgtatgta gtaggtgcat
    29581 tacctttact attattggca gtcgtagcga ttgctaaggt gtctgaaccg ttatctaaaa
    29641 agcaacaaaa aaacttagat aaaatcaaca gtatcttgcg ggaaaatctt tcaggattgc
    29701 gtgtgattcg tgcattcgtc aatgaaaagt ttgaagaaaa acgttttgct gtagtcaatg
    29761 agaattatgc ttctagttca aaaagcttat tccgtttgat ggctgttgca caaccgggtt
    29821 tttatttctt atttaatatc gtaatggtat tgatcatctg gagcggggca gtgcaaatcg
    29881 atgcaggcga gcttcgtgta ggagatttga ttgcgtttat cgagtatata ttccatgcct
    29941 tgttctcatt catgttattt gctagtgtgt tcatgatgta tccaagagca gctgtttcag
    30001 ctggacgtat ccaagaagcc ctagatgcca aaccttcgat tgtagaagat ccaaatggta
    30061 tatctgatac aaaaactaaa gggtatatcg aatttcaaaa tgtaacattt gcttatccag
    30121 gccattcgca agagccagtg atacgaaatg tcagttttac agcctctcca ggagagaccg
    30181 ttgcttttat tggaagtacc ggtagcggaa aatctacgtt gatccaattg atccctcgct
    30241 tttatgatgt gagtgaagga aagatcttga tcgatggagt agacgtgaga gattatcggc
    30301 taagcaagtt gcgagataag attgggttta ttccgcaaaa agcgctgcta tttactggaa
    30361 cgattgcaga taatttgcga tacgggaaag aagatgctac gcaagaagaa atggaacgtg
    30421 cagctgagat tgctcaagct tcagaattca tttctcgaaa accagatgga tttaatgaac
    30481 atttatcaga aggtggttcc aatttttcag gtggacaaaa acaacgattg gcaatagcca
    30541 gagccatcat acgccgtccg gaaatctata tttttgatga cagcttttct gcattagatt
    30601 atcaaacgga tgcgaagtta cgtgctcgtc tgaaaaaaga aacgacagaa tctactgtat
    30661 tgatcgtagc acaacgtgtg ggaacgatca tgcatgcaga taagatcatc gtattaaatg
    30721 aaggagaagt cgtcggtatg gggacgcatc gggagttgtt ggaaaaatgc ccgatttatt
    30781 atgatattgc cgcttctcaa ttatcaaagg aggaattagc atgaaaaaaa ccttttcttc
    30841 cttaaaacgt ttagctcgat atatcagacc gtatcgtgag acttttcttt tagtgatcct
    30901 atttacagtt ctgacagtgg ctttcaacgt ggctttgcca tatgttacag gacttcctac
    30961 tacagaaatc agccgtaata ttgccaatgg cgaatcaatc aatttttctt atatattcaa
    31021 atgtctcatt tggatcacag ttgttggaat agggtattgt atttcgcaat tactttctgg
    31081 tgttttgatg acaaatgtcg ttcaaagtgc catgcatgat ttgagaagag acatagaaga
    31141 aaaaatcaat cgtcttccgg tttcatattt cgataaaaac cagcaaggaa atattttgtc
    31201 tcgtgtgacg aatgatgtag atgcagtcag cggtgcttta caacaagctt ttattggtat
    31261 cgtaaatgcg atattgggaa ttacgttggc tgcagcaatg atgttttata tccagccgat
    31321 gatggcgctg atctctatga tcatgattcc aacatcgatc tggatctcaa aacgagtgat
    31381 caatgcttcg cagaaatatt ttcaaagcat gcagaattct cttggtgagc taaacggcta
    31441 cgtccaagaa aatatgacag gttttagtgt attgaaagta tatggacgcg aaaaagaaac
    31501 attggaagga ttcaaaaaag ttaatcacag cttgaaatac tttgggtttc gtgcagcatt
    31561 catttctggc ttgatgatgc ctctagttca attaactgcc tatgctactt atattggtat
    31621 ggctgttcta ggaagctttt atgttattac tggggcgatc gttgtcggtc aactacaagc
    31681 attcatccaa tatatttggc aaatcagtca gccaatgggg aacgtgacac aattgtcttc
    31741 agttttgcaa agtgcatcag cggcaacgaa acgtgtattc gagattttag atgagccaga
    31801 agaaaaagaa aacaaagtag atgcttcact gccagaaacg attttaggag atgtgacatt
    31861 cgatcatgtc gattttgcat atgatccgaa gaaaccatta atccaagatt tgaattttga
    31921 agtgaaagct ggtcaaaccg tagctgtggt cggtccgacc ggtgccggca agacgacatt
    31981 gatcaatcta ttgatgcgtt tttatgacgt agacaaagga gcaataagga ttgatggaat
    32041 cgacaccaaa tcaatgagtc gcagtgatgt gcgttcgcta tttggtatgg tcttgcaaga
    32101 tgcatggctt tatcaaggaa cgattgcaga taatatccgc ttcggaaagc ttgatgcgac
    32161 agattatgaa gtagttgatg ctgcgaaaac agcaaacgta gatcatttta tccgaacgat
    32221 gccagatgga tatgatatgg ccatcaattc tgagggagac aatgtgtctt taggacaaaa
    32281 acagttgctg acgattgccc gagcagtggt ctccgatcct aaaatcttga ttttagatga
    32341 agcaacaagt tcagtagaca cccgtttaga agcgttgatt caaaaagcaa tggatcgcgt
    32401 gatggaggga cggacaagtt tcgtcatcgc tcaccgttta tcgacaatcc gtgatgcaga
    32461 tctgattcta gtgatggatc aaggtcagat cattgaaaaa ggaacgcatg agagtttatt
    32521 ggcacaaggt ggattttatg aaaaactgta taacagccaa tttgctgagg aagcagaata
    32581 agtcatataa gaataaaaca gtttaaaact tattttttgt tctctattct tgataatggt
    32641 aaagtaaagt tatcaagaaa agtgaggaca cggagatgga acagttttta aatgaaaaaa
    32701 ttactgaagc caaaagagaa gttgatcgtt taacgcacgt ctgtcagacg aagccttttg
    32761 attcagctga tttttttgaa aatgagtttc aaatcgaacg cttggcagca caaatagaag
    32821 tgtacgaatt aattttaaaa aaagtaatag aagaaaatat agatgtacaa taaaaacagg
    32881 taaaacgtca gttaccagat actgtaaata gacatctggc ctgagcaggt tttacctgtt
    32941 ttttttctta tgaaataaat atatgctatg atacctttat tcaataggat tagtttctac
    33001 aaaaaatgaa aggagaaaaa taaatatgga acggtttata gcatcaaatg ccacagtgat
    33061 tggtgatgtg acattgtcag aagatgtgac gatctggtat caagctgttt tacgcgggga
    33121 cagtaattgg ataaaaatag gacaaagaac gaatatccaa gatggtacaa tcatccatgt
    33181 cgatcatgat gcacctgtag atattgcgga gaatgtgacg gtaggacatc agtgcatgct
    33241 acatggctgt acaatcgaaa aaggggcgct gatcgggatg gggaccacga tattgaatca
    33301 tgcggttatt ggtgagaata gtttgattgg tgctggatca ttagtaacag aaggaaaagt
    33361 tattcctcct aatgtcttgg cttttggtcg cccagctaaa gtgatccgtc ctttgacaaa
    33421 agaagaaata caaaaaaaca aagaaaacat ccagcattat gtcgagatag gtcggatgca
    33481 tgcgcaagga aaattcaagg agcaaagata ggcagtagca tccataaaat aaaaaaggat
    33541 ggtcagtttg agttgaccat ccttttttat ttggtttaga taagagagct taaaaaagct
    33601 tcctttgaaa cagattaatt taatgaagaa ctgttttcca tgacttcatc gatcaatcca
    33661 tatgcttttg cttgttcagc tgtcatatag ttgtcacgat ctgtatcttt ttcaatcact
    33721 tcaagcggtt gtccagtgcg ttcagctaaa attttgttta agcgttcgcg tgtttgcaaa
    33781 atatggcgtg ccgcaatttc gatttcagtt gcttgccctt gagcgccgcc aagaggttga
    33841 tggatcatga tctcagcatt tggtaaagca aatcgtttac cttttgtacc agctgtcagc
    33901 aagaagcttc ccattgaagc agccataccc ataacaattg tttggacatc tgctttgaca
    33961 aagttcattg tgtcgtaaat agccatacct gctgtaactg aaccaccagg agaattgata
    34021 taaagataga tatctttttc agaatcttga gcgtctaaga aaagcaattg ggcgatgatc
    34081 gaattagcaa gatcatctgt tacttggccg cttaacatga tgatacggtc tttcaataaa
    34141 cgtgagtaga tatcgtaagc tctttcacct cgtgaagatt gttcaatgac tgtaggaatt
    34201 aaattcataa tagagttcct ccttgatttc gtttagaaat agtgtaccat aatttagttt
    34261 ctaataccta aaatgtagta tacatttttg gtcaaaaaag gtcaaataaa aaacttaggc
    34321 aaattagtcg cataaacccg ctttatggta tgataagcag agaaacaaaa caaggaggaa
    34381 caacagattg atagttggta ttactggcct tatcggagtg ctgacaggtg ctgtagttgt
    34441 ctatatcggc atgatactgc aaatgcgtca cttggaaaat aaagaacagc gtagacgtga
    34501 attggaattg aaagtcaaag aaatcgagac gctgaatcaa ttaaataaaa agatcaacga
    34561 gatcttgcaa aaacggacaa cgctgatgga agaatatgtc agctttgatg catttgatga
    34621 ttgttatatc acgattgacg attttgcgta tctccaatca ttttcagcgc aaaatagttt
    34681 ttatctgcca aattatattt tagacgagct atttaagaat atctcccatc gaaaagtgat
    34741 tttatctcca gatgaaacaa tgaagattgg tggatatgct tataaaggcg gacgcgtgat
    34801 tttagagaat tttacggata gtctgactga aatgatcaat gaaaaaaaag ctcagttgaa
    34861 gactatgacg cgccagccga ttggtttctt ctcgaaagat gatgtttgga gctgatattt
    34921 tgaagcattg ccgttgcaca tgagagcgtg gtaatgcttt ctttttttct taatgataca
    34981 atagaactga acggagtgaa gaaagatgtt acaaatctat gcccatcgcg gaagcagcgg
    35041 cacccaccca gaaaatacgc ttcctgcatt tgcagaagct gtacgagtag gtgcagatgg
    35101 tatcgaacta gatgtccatc taagcaaaga tggttatttg atcgttatgc acgacgaaga
    35161 agtggaccgg acgactaacg gtaaagggct gattcgagaa aaaacattgg aagaactcaa
    35221 aaaattgaat gcagggggca aattcagcaa agaatttcca gcagctaaag ttcctgaatt
    35281 gagagaagtg ttaggactgc tcctccaaaa aaattaccga gggatgctaa tgattgaaat
    35341 taaaactgat cagtatgaat atcccggtat cgaagaaaaa ttagccgaaa cattaaaaag
    35401 tagagattgg ccttttcaac atgcatattg cagctttcat ttggattctt tggaaaaaat
    35461 cgcattactg gaaccggaga tacagctgga cttattgatg agtacttctg ataaaaagat
    35521 ccaactagca gcagaaaccc cttatataga aggagtacat ccaaaaaata cttggttgtt
    35581 cgaacatcca gtccaggcag ctgattttcc aaaagcaatt cgtgcatgga cgattaacga
    35641 tgaagaagaa atggccaaat gtcaagaact caaaatagat ggaataataa ctgattttcc
    35701 ggagaaagct cttcggtgga aacaatcaac gaaaagtagg taaaatatgg aaaaagtatt
    35761 agtgatcgtc ggtccgacag cagtcggtaa aacagcgcta agtgtggaac tcgcacaact
    35821 atttggagga gagatcatca gtggtgattc attgcaaatc tataaaaatc tggatatcgg
    35881 tacagcaaaa atcaaagaag aagagatgaa taatgttccg catcatttga ttgatgtgat
    35941 cgaacctact caacgttact cagccgctga ttttcagcat tcaggtcgac aattgattac
    36001 cgagattgcg aaccgagggc acctacctat catagctggt ggtacgggtc tctatatcca
    36061 atcccttcta tatgattatc agttaggagc gacagaagaa gcgaatcctg agattagaaa
    36121 aaagtatgaa acgttagctg aaacaattgg ttcccaagca ttatggaatc agttgaaaga
    36181 aaaagacccg gcagctgctg agaaaatcca ctggaacaat caacgcaaag tgataagagc
    36241 tctggaagtt ttagagacga caggacatag tatcacggca cctaaagaag agccaaagca
    36301 attgtatgat tacctcatga ttggattaga tacgaagcgt agtattcttt atgaacgaat
    36361 caatcaaaga gtcgatttga tgctagcaga aggtttagta gcagaagcgc gtaaggtgta
    36421 tgagcttgga gaagtccagg ccagtcaagg tattggatat aaagaatttt tcccttattt
    36481 tgaagggaaa gaaacattgg aagaagcaac agaacaagtc aaactacatt cgagaagata
    36541 cgcgaaacgt cagttgacgt ggtttcgtaa ccgtttggat gcacagtggt ttgatcttat
    36601 aagtgagcca aatcaaaaaa gtcagattga gcaaacaatc aaaaaatggc tggaggcgga
    36661 ataaaatgga agaaaaagtg atcatcgtag gggtagaaac cgaagcaaac cagcgttact
    36721 tttcagagtc aatggaagag ctagttcagc tgacgaatac cgcatcagga gaagtcgtat
    36781 tcacaattac acaaaagaga ccacaagtgg atcggcaaac gattatcggt aaagggaaat
    36841 tacaagaatt agttcaacaa gcggatgcgc atgaagcgga tttgatcatc tttaatcatg
    36901 agctaactcc gagacagagc caactgatta ccgatgctgt tggtcttccg gtaattgatc
    36961 gcgtgcagct tatcttagat atttttgcga tgcgtgctcg ttcgaaagaa gggaagctgc
    37021 aggttgaact tgctcagtta gaatacctgc tgcctcgttt agtagggcaa gggaaaacat
    37081 tatctcgtct tggcggtggg atcggaacaa gagggccagg tgaaacaaag ttagaaacag
    37141 accgccgaca tatacggaat aaaatttttg ctgttaagaa agaattgaaa gaagtagaag
    37201 ctcatcgaga gcgcaatcgc caaaaaagaa aaaatagcga gatttttcag attggactta
    37261 ttggctacac gaatgccggg aagtcaacta tcttgaatct attgacacaa gcggatactt
    37321 attcgaaaga tcaattgttt gcaacgttag atccattgac taagagatgg cgttttgctg
    37381 aaggatttga gattactgta acggataccg tcgggttcat ccaagacctg cctacgcaac
    37441 tgatcgatgc tttccattct acattagaag aaagtcaaaa tatggatctt ttgctacatg
    37501 tagtcgatgc tagttctcca gatcgtattt tacaggagca gactgtctta aaattgatgg
    37561 atgaactgaa tatgaaagaa atgccgatac tgactgttta caataaggca gaccagatcg
    37621 acccagcgat gttcacgcct acgctattcc caaatgtatt gatttctact cagacgaaag
    37681 aaggtaagat ggcgttgatc gaagcaatca aacgtcagct gatggaacta atgacccctt
    37741 atacactttc tgtgccaagc aacgaaggac agaaattaag cgagttgaga agacaaacaa
    37801 tggttttgga cgaagcgtac atggaagaaa caaacgaata tgaagttaaa gggtttgcaa
    37861 aaaccaattc aaaatggtta agaaaaaacc aataaaattt tattttttta a
//
LOCUS       DANJJW010000014        35398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000014 DANJJW010000000
VERSION     DANJJW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            8..649
                     /locus_tag="PWS82_000703"
     CDS             8..649
                     /locus_tag="PWS82_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905851.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="PWS82_000704"
     CDS             708..1148
                     /locus_tag="PWS82_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905852.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="PWS82_000705"
     CDS             1160..1828
                     /locus_tag="PWS82_000705"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0905853.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="PWS82_000706"
     CDS             1892..2515
                     /locus_tag="PWS82_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL0905854.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="PWS82_000707"
     CDS             2571..2957
                     /locus_tag="PWS82_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL0905855.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="PWS82_000708"
     CDS             3170..4561
                     /locus_tag="PWS82_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL0905856.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="PWS82_000709"
     CDS             5172..5540
                     /locus_tag="PWS82_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL0905857.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="PWS82_000710"
     CDS             5720..6661
                     /locus_tag="PWS82_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL0905858.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="PWS82_000711"
     CDS             6730..7461
                     /locus_tag="PWS82_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL0905859.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="PWS82_000712"
     CDS             7605..9785
                     /locus_tag="PWS82_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL0905860.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="PWS82_000713"
     CDS             9805..10287
                     /locus_tag="PWS82_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL0905861.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="PWS82_000714"
     CDS             10303..11064
                     /locus_tag="PWS82_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0905862.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="PWS82_000715"
     CDS             11195..12529
                     /locus_tag="PWS82_000715"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL0905863.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="PWS82_000716"
     CDS             12836..13321
                     /locus_tag="PWS82_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL0905864.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="PWS82_000717"
     CDS             13314..13997
                     /locus_tag="PWS82_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL0905865.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="PWS82_000718"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="PWS82_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL0905866.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="PWS82_000719"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="PWS82_000719"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL0905867.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="PWS82_000720"
     CDS             17613..18242
                     /locus_tag="PWS82_000720"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL0905868.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="PWS82_000721"
     CDS             18294..18626
                     /locus_tag="PWS82_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0905869.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="PWS82_000722"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="PWS82_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL0905870.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="PWS82_000723"
     CDS             19452..20825
                     /locus_tag="PWS82_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0905871.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="PWS82_000724"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="PWS82_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL0905872.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="PWS82_000725"
     CDS             21880..22320
                     /locus_tag="PWS82_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0905873.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="PWS82_000726"
     CDS             22464..23165
                     /locus_tag="PWS82_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL0905874.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="PWS82_000727"
     CDS             23162..24283
                     /locus_tag="PWS82_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL0905875.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="PWS82_000728"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="PWS82_000728"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL0905876.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="PWS82_000729"
     CDS             25662..26570
                     /locus_tag="PWS82_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL0905877.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="PWS82_000730"
     CDS             26605..26877
                     /locus_tag="PWS82_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL0905878.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="PWS82_000731"
     CDS             26888..27934
                     /locus_tag="PWS82_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL0905879.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="PWS82_000732"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="PWS82_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL0905880.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="PWS82_000733"
     CDS             complement(30945..31631)
                     /locus_tag="PWS82_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905881.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="PWS82_000734"
     CDS             complement(31609..32103)
                     /locus_tag="PWS82_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0905882.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="PWS82_000735"
     CDS             complement(32123..33343)
                     /locus_tag="PWS82_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905883.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="PWS82_000736"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="PWS82_000736"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL0905884.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DANJJW010000015        35171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000015 DANJJW010000000
VERSION     DANJJW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            164..1309
                     /locus_tag="PWS82_000737"
     CDS             164..1309
                     /locus_tag="PWS82_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0905885.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="PWS82_000738"
     CDS             1446..1790
                     /locus_tag="PWS82_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL0905886.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="PWS82_000739"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="PWS82_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL0905887.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="PWS82_000740"
     CDS             complement(3485..4084)
                     /locus_tag="PWS82_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL0905888.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="PWS82_000741"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="PWS82_000741"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL0905889.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="PWS82_000742"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="PWS82_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL0905890.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="PWS82_000743"
     CDS             7290..8408
                     /locus_tag="PWS82_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL0905891.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="PWS82_000744"
     CDS             complement(8468..9760)
                     /locus_tag="PWS82_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0905892.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="PWS82_000745"
     CDS             9892..10470
                     /locus_tag="PWS82_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0905893.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="PWS82_000746"
     CDS             10599..10976
                     /locus_tag="PWS82_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL0905894.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="PWS82_000747"
     CDS             10909..12981
                     /locus_tag="PWS82_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0905895.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="PWS82_000748"
     CDS             complement(13192..14139)
                     /locus_tag="PWS82_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0905896.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="PWS82_000749"
     CDS             14385..14849
                     /locus_tag="PWS82_000749"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL0905897.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="PWS82_000750"
     CDS             complement(14911..15954)
                     /locus_tag="PWS82_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL0905898.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="PWS82_000751"
     CDS             complement(16331..17296)
                     /locus_tag="PWS82_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL0905899.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="PWS82_000752"
     CDS             17530..18375
                     /locus_tag="PWS82_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0905900.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="PWS82_000753"
     CDS             18566..19753
                     /locus_tag="PWS82_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0905901.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="PWS82_000754"
     CDS             19759..20115
                     /locus_tag="PWS82_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL0905902.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="PWS82_000755"
     CDS             20117..20455
                     /locus_tag="PWS82_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL0905903.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="PWS82_000756"
     CDS             20850..21884
                     /locus_tag="PWS82_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0905904.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="PWS82_000757"
     CDS             21877..22563
                     /locus_tag="PWS82_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0905905.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="PWS82_000758"
     CDS             22587..23435
                     /locus_tag="PWS82_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0905906.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="PWS82_000759"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="PWS82_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL0905907.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="PWS82_000760"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="PWS82_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL0905908.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="PWS82_000761"
     CDS             25701..26936
                     /locus_tag="PWS82_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0905909.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="PWS82_000762"
     CDS             26923..27393
                     /locus_tag="PWS82_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL0905910.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="PWS82_000763"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="PWS82_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL0905911.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="PWS82_000764"
     CDS             complement(28884..30026)
                     /locus_tag="PWS82_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0905912.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="PWS82_000765"
     CDS             complement(30065..30799)
                     /locus_tag="PWS82_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905913.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="PWS82_000766"
     CDS             31031..31306
                     /locus_tag="PWS82_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905914.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="PWS82_000767"
     CDS             31404..33401
                     /locus_tag="PWS82_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905915.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="PWS82_000768"
     CDS             complement(33477..33812)
                     /locus_tag="PWS82_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905916.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="PWS82_000769"
     CDS             33939..34154
                     /locus_tag="PWS82_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905917.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="PWS82_000770"
     CDS             34191..34553
                     /locus_tag="PWS82_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL0905918.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="PWS82_000771"
     CDS             34599..34925
                     /locus_tag="PWS82_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905919.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11591 a         6224 c         7364 g         9992 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg aacaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta
    35041 caatgattgt cgggaatttt tatgttattc actgttataa caatataaaa gaaatgaaaa
    35101 agaaaaaatt attatggata tgtttggtat ttttattgat aattggtgga attgaaatac
    35161 ttactacatg t
//
LOCUS       DANJJW010000016        35137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000016 DANJJW010000000
VERSION     DANJJW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..174)
                     /locus_tag="PWS82_000772"
     CDS             complement(<1..174)
                     /locus_tag="PWS82_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0905920.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGL"
     gene            491..2122
                     /locus_tag="PWS82_000773"
     CDS             491..2122
                     /locus_tag="PWS82_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0905921.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2125..3204
                     /gene="uxuA"
                     /locus_tag="PWS82_000774"
     CDS             2125..3204
                     /gene="uxuA"
                     /locus_tag="PWS82_000774"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0905922.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            3278..4705
                     /gene="uxaC"
                     /locus_tag="PWS82_000775"
     CDS             3278..4705
                     /gene="uxaC"
                     /locus_tag="PWS82_000775"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0905923.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4789..5436
                     /locus_tag="PWS82_000776"
     CDS             4789..5436
                     /locus_tag="PWS82_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL0905924.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5565..6941
                     /locus_tag="PWS82_000777"
     CDS             5565..6941
                     /locus_tag="PWS82_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0905925.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6952..7746
                     /locus_tag="PWS82_000778"
     CDS             6952..7746
                     /locus_tag="PWS82_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0905926.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            7759..9525
                     /locus_tag="PWS82_000779"
     CDS             7759..9525
                     /locus_tag="PWS82_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL0905927.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            9553..11361
                     /gene="uidA"
                     /locus_tag="PWS82_000780"
     CDS             9553..11361
                     /gene="uidA"
                     /locus_tag="PWS82_000780"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL0905928.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            11533..11934
                     /locus_tag="PWS82_000781"
     CDS             11533..11934
                     /locus_tag="PWS82_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0905929.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            11928..12173
                     /locus_tag="PWS82_000782"
     CDS             11928..12173
                     /locus_tag="PWS82_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905930.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(12497..12718)
                     /locus_tag="PWS82_000783"
     CDS             complement(12497..12718)
                     /locus_tag="PWS82_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905931.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(12880..12952)
                     /locus_tag="PWS82_000784"
     tRNA            complement(12880..12952)
                     /locus_tag="PWS82_000784"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12917..12919),aa:Lys,seq:ctt)
     gene            13028..13258
                     /locus_tag="PWS82_000785"
     CDS             13028..13258
                     /locus_tag="PWS82_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905932.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            13302..14471
                     /locus_tag="PWS82_000786"
     CDS             13302..14471
                     /locus_tag="PWS82_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0905933.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            15045..15614
                     /locus_tag="PWS82_000787"
     CDS             15045..15614
                     /locus_tag="PWS82_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL0905934.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            15601..16461
                     /locus_tag="PWS82_000788"
     CDS             15601..16461
                     /locus_tag="PWS82_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0905935.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            16651..17355
                     /locus_tag="PWS82_000789"
     CDS             16651..17355
                     /locus_tag="PWS82_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0905936.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            17360..19195
                     /gene="walK"
                     /locus_tag="PWS82_000790"
     CDS             17360..19195
                     /gene="walK"
                     /locus_tag="PWS82_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL0905937.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            19192..20508
                     /locus_tag="PWS82_000791"
     CDS             19192..20508
                     /locus_tag="PWS82_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905938.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            20509..21378
                     /locus_tag="PWS82_000792"
     CDS             20509..21378
                     /locus_tag="PWS82_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL0905939.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            21433..22242
                     /locus_tag="PWS82_000793"
     CDS             21433..22242
                     /locus_tag="PWS82_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0905940.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(22345..22974)
                     /locus_tag="PWS82_000794"
     CDS             complement(22345..22974)
                     /locus_tag="PWS82_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL0905941.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            23144..24436
                     /locus_tag="PWS82_000795"
     CDS             23144..24436
                     /locus_tag="PWS82_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0905942.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(24512..25174)
                     /locus_tag="PWS82_000796"
     CDS             complement(24512..25174)
                     /locus_tag="PWS82_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0905943.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            25399..25683
                     /gene="groES"
                     /locus_tag="PWS82_000797"
     CDS             25399..25683
                     /gene="groES"
                     /locus_tag="PWS82_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL0905944.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            25734..27359
                     /gene="groL"
                     /locus_tag="PWS82_000798"
     CDS             25734..27359
                     /gene="groL"
                     /locus_tag="PWS82_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL0905945.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(27448..28596)
                     /locus_tag="PWS82_000799"
     CDS             complement(27448..28596)
                     /locus_tag="PWS82_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0905946.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(28698..29282)
                     /locus_tag="PWS82_000800"
     CDS             complement(28698..29282)
                     /locus_tag="PWS82_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0905947.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(29409..29840)
                     /locus_tag="PWS82_000801"
     CDS             complement(29409..29840)
                     /locus_tag="PWS82_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0905948.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(29858..30178)
                     /locus_tag="PWS82_000802"
     CDS             complement(29858..30178)
                     /locus_tag="PWS82_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0905949.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            30478..30618
                     /locus_tag="PWS82_000803"
     CDS             30478..30618
                     /locus_tag="PWS82_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905950.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            30916..31173
                     /locus_tag="PWS82_000804"
     CDS             30916..31173
                     /locus_tag="PWS82_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDL0905951.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(31144..31452)
                     /locus_tag="PWS82_000805"
     CDS             complement(31144..31452)
                     /locus_tag="PWS82_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905952.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            31530..32276
                     /locus_tag="PWS82_000806"
     CDS             31530..32276
                     /locus_tag="PWS82_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL0905953.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            32291..32494
                     /locus_tag="PWS82_000807"
     CDS             32291..32494
                     /locus_tag="PWS82_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0905954.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            32599..32898
                     /locus_tag="PWS82_000808"
     CDS             32599..32898
                     /locus_tag="PWS82_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905955.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            32898..33080
                     /locus_tag="PWS82_000809"
     CDS             32898..33080
                     /locus_tag="PWS82_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905956.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(33086..33562)
                     /locus_tag="PWS82_000810"
     CDS             complement(33086..33562)
                     /locus_tag="PWS82_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905957.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            33615..34340
                     /locus_tag="PWS82_000811"
     CDS             33615..34340
                     /locus_tag="PWS82_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0905958.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            34514..34855
                     /locus_tag="PWS82_000812"
     CDS             34514..34855
                     /locus_tag="PWS82_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905959.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            34848..>35137
                     /locus_tag="PWS82_000813"
     CDS             34848..>35137
                     /locus_tag="PWS82_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0905960.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        12169 a         5374 c         6989 g        10605 t
ORIGIN      
        1 aagtccttga tgacggagtt gaatcaatgc ttcacgtatt ggagtacgac cgatagaaag
       61 cgtttcttcc aatttttttt ctgatacttt ttttccaggt tctaagtcag cgtagataat
      121 ttgctgacga atagattgat aagcttgatt ttggagacta gttttaattg tcatatttaa
      181 tccctctcaa aatttcattt ttgaatttga tattttagtt ctatgctcgt tatataatat
      241 caaatttcaa agtaacatac tagctattaa aaaaacaaaa aatactcaaa taagatcaaa
      301 gagaaattaa gctttaattt aagttagttc taaaaaatat atattggtgt gcttttacta
      361 aaaaaagaca caacaattct gacatggtag aaaaaagaat atttttagtt ttttcttatt
      421 gacagcgatt acattcgatt ttatactaca attgtaacgg aaactaacat atcagtatgg
      481 aggggtaaat atggttttga ttaaagactg gcaagattct atcgaaggat tgaacaaaat
      541 gcaagttcaa ctaccgtatt ttaatgtcga agcggtcaaa caagaaggtc agaaacatcc
      601 agtgtggctt cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca
      661 atgcttagca gatcaaaatg aattagaatc cggagtagtt gtgtgtgaga catttgatga
      721 agaagtagta gaaaaagctt atcatgacta caacaatgat attttggaga ttgttatggg
      781 tgaagatggt gaactcaaaa aaagactttt agcagtaaca gctgatagtt tatattgcaa
      841 tccaaagaat ataacggact atcaaagagt ggttaaatat tttgaagatc ctacattaca
      901 gtttacaacg tttactatta ctgaaaaagg atacagttta aaagattcta aaggacaatt
      961 tttttcggta gtggaagcag atataaaatc tggacctacc aatcctcaac atacgatgag
     1021 tattgtgaca tccttgttgt atagcaggtt taatgctgga gagttaccga ttgcgatggt
     1081 ttctactgat aatttctcac agaatggtca acgttttcaa gatagcattt ttatgattgc
     1141 agaagcttgg caaaaaaaag gatacgtttc tgaaaaattt attgcttatt taaaggataa
     1201 agaaaaagtg acttttcctt ggagtatgat tgatagaatt acgccaaatc cttcggaaag
     1261 tgtcaaaatg gatttattga aggcgggttt cgaagactta gatataattc atactgcgaa
     1321 aaataccaat atcgctgctt ttaccaatac tgaaaaagtc cattatctag tcattgaaga
     1381 tagttttcca aatggtcgac ctaaactgga agcggctggt gtgattatga cagatagaga
     1441 aacagttgat aaagcagata tcatgaaggt tacgacatgt ttaaatccgc tccatacggc
     1501 attagcaata acaggttgtt tgttggggta taaatcaatt caggcagaaa tgcaagatga
     1561 tgatttggtt ggcttaatta aagggatagg ttatgtagaa ggcttaccag tagttgaaag
     1621 tccaggcgtt attgatccaa aacaatttat tgatgaagta atcaataaac gtttgcctaa
     1681 tccctatatt ccagatacac cgcaacgaat tgcaacagat acttctcaaa aagtagcgat
     1741 tcgctatggt gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac
     1801 atttattcct atcgctattg ctgcttggtt acgttattta ttggcagtcg atgatcaagg
     1861 aaaaaatttt gtgccaagtc ctgatccttt attggtagag ttgcaggaaa aattctcagt
     1921 attgaaatta ggtgaacaag atcctgcaaa gattcattgt gcggtacagg aaatcttaga
     1981 aaataaggaa atatttggtt tagatttata tcaagttgag ataggaaaga aaattgaaaa
     2041 aatcttttgc gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat
     2101 aacagaagtt ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa
     2161 gaaaaaatct cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca
     2221 ttaatggata taccagccgg agaggtctgg ccgagagaac gaattaaagc tttaaaagat
     2281 gaagtcgaag ccaatggcct taccttgaaa gttattgaga gtgttaatgt tcatgatgat
     2341 atcaaaattg gtttgccaac acgagatcaa tacatcgaga attataaaca gacgattcgc
     2401 aatttggcag aatttgggat tgaggttatc tgttataatt ttatgccagt ctttgattgg
     2461 gtaaaatcag atttggatta tcatttagat gatgggtctt ctacgttagc ctatatttca
     2521 aaaaatattc ctactgatcc agaggaaatt attagaaaag tagaaaattc tactaataat
     2581 tttgaattgc cgggttggga acctcatcgg ttatcacaaa tcaagcaatt attagaagcc
     2641 tataaagaag ttgatgaagc gaaattgcga gagaactttg catattttct caaagcaatt
     2701 atcccgacat gtgaagaagt tgggattaaa atggcagttc atccagatga tccaccatat
     2761 tctattttcg gattacctcg tattgttaaa aatcgtgacg atttagaatg gttgtgtaat
     2821 gtagtggatt ctccttctaa tggtattacg ttatgtactg gaagtattgc agaacgacca
     2881 gataatgatg tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca
     2941 catgttcgta atatcaaata tttaagtgat aatggactcg atttttatga agcgccgcat
     3001 aaatcagaat atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga
     3061 tttaatggct atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct
     3121 ggttatgggt tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg
     3181 cttgaaaagg gggcgagtaa ataattatat aatggagtct atttagaaga aaaattttct
     3241 cttctaaaaa ctaagggaag gaaaggagtg cttaaatatg tttttagata agaacttttt
     3301 attagagaat gaaatagcta aaagactttt tcataaattt gcagaacacc aaccaatcat
     3361 cgattatcat tgtcatttag acccagcatg tttgctagaa aataagcaat acaagaattt
     3421 gactcaggta tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg
     3481 agctaatggg atagaagaag agttgatcag tggtgcaggt aatgattatg aaaaattttt
     3541 agcttttgta aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt
     3601 ggaattacaa cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat
     3661 ttggcgacgt gccaacgaaa aaatccaaac ggattcctat cgcccaaaag aattgctgaa
     3721 gatgatggat gtgaaagcca tttgtacaac ggatgatcct acagatgact tgcgatatca
     3781 tactgagctt tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa
     3841 ggtctggagt atcaaatcat caggattttc gaactatatt ttaaaactta gtcaagcagc
     3901 gggtatacca atcaaaaact ttaatgggtt gaagcaggca ttgagccaac gagtggatta
     3961 ttttcatcaa attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat
     4021 agcagatgaa aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt
     4081 ccatttatca gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta
     4141 tacaagatat ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat
     4201 attaaaggaa caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt
     4261 gacacaaaat gttgtagcat tgttcgacac tgctgaaaag agtgggtgct taccaaaaac
     4321 aattttatat tcattaaatc caaccgattg gcttccacta gcaacagcaa tgcaaagctt
     4381 ccaagggggt atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc
     4441 tgaaggaatg cgtcaacagt tgactacgat ggcgcaacaa agtttgttgt cgaattttac
     4501 aggtatgttg actgattctc gaagttttct aagctatcct cgccatgagt atttccgtcg
     4561 agtattatgt caattactaa gcgagtgggt taaagcaggt cgaattccag atgatgaaat
     4621 gtatcttagt cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt
     4681 ttttgaaatc gaaagtagaa aataatacaa aaaataaaag cttaaattta tttattgtaa
     4741 gttagtacaa ctagctagag tatgtatcca taaaagaagg agatagatat gaaaaaagta
     4801 gaaattttaa gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg aaaaaataaa
     4861 gatgaggcca ttaaagcaat aaatgcaatt actgcgggtg gaattaaagg gattgaacta
     4921 acttttacag ttccgaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat
     4981 caaaaagatg taataattgg agccggtaca gttttagatg cgtttactgc tcgtattgca
     5041 attatggctg gagcagaata tatcgtaagt ccatcttttg atcgacaaac agcagagatt
     5101 tgtaatttat atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag
     5161 gcacttaccg ctggagtaga aatcattaaa ctatttccag gttctgcttt tggtccatct
     5221 gttgtaaaaa cgtatcaagc tccattacca tatgtaaata taatgcctac tggcggagta
     5281 agtctagata acttagaaga gtggtttgaa gcaggcgttg ttgcagttgg tgtaggtggg
     5341 aatttgttgg ctcctgttga aaaaggtgat tttgaagaag taactcgaat agctagtaaa
     5401 tatatagata agttttatga gataaagaat tattaggaga aaaaataaat aatactataa
     5461 aatgtaaaaa ataaatacta actctttata cttcagagta tagtacgtaa gttggcagtt
     5521 caacagaatg aaaacgctta acaataggaa ggagcgcaaa ttatatggaa agaaaatttg
     5581 gtctaagaga caaaatagga tatatgtttg gagattttgg gaatgatttc actttcattt
     5641 ttgctagttc ttttttaatg attttttata caaaagtatt ggggataagc ggagctaccg
     5701 taggaacatt atttttagta gcaagattta ttgatgcagt tacagacata acaatggggc
     5761 gtattgtgga tggatcaaaa ccatctaaaa ctggcaaatt taaaccgtgg attttacgga
     5821 tgagtggtcc agtcgcttta gcaagttttt taatgtatca aacatcaatg gcatcagctt
     5881 ctatgagtat taaagttata tatatgtatg taacatatat actatgggga agtatttttt
     5941 atacatctat taatattcct tacggttcaa tggcatcagc tatcacttct gttgcagaag
     6001 aaaggacagc gttatcaaca tttcgtagta taggagctac cttagcaggt atgttcattg
     6061 gcgtgttaac accaattttt atttatacaa gagatagttc tggagctcaa attgtacgtg
     6121 gtggatcaac ttttacaatt gtggcaggag tattttcagt gtgtgcagtt atttgttacg
     6181 taatatgtta taaattaact gtagaacggg ttaaaagtga tgaatcgcag ttaaaagttt
     6241 cattgttgaa atctttaaaa ggattagcta aaaatagagc gttgctttct ataatagctg
     6301 cagctatttt tttgttgctt gctcagcttt taacaatgag tatgaacaat tatgtatttc
     6361 cagattatta taataatgca cagggaatag ttttaatgaa ctttatcaat cctattttga
     6421 tgttggtact tgtttcacct gtaagtttaa ttttcagtaa aaaatacggt aaaaaagaat
     6481 tggcgtcagt agggatgttc ttctctgcta ttgtatattt acttttattt ttgataaagc
     6541 ctagtaatat gtatgtattc ttagttctta cttctgttgg atatatgggg ttaggtattt
     6601 ttaatacagt catttgggcg aatattactg atgtaatcga tgatcaagaa gtgaaatctg
     6661 gccaaagaga agatggaact atttatgcag tatattcttt tgcccgaaaa attggacaag
     6721 ctttagctgg cggtgtcgga ggttggactc tttctataat agggtatgat tcgttagcaa
     6781 aagtacaaag tggagaagtt ttacaaaagc tgtataactc ttcaacgttg attccagcag
     6841 tttgtttctt tgttgtagga ttgattttat ttttcgtata tccattatct aaagaaaaag
     6901 tattagaaaa ttataatttg ctaaatcaaa aacataacta ggagaaaaaa catgaacagt
     6961 tggatatttt gtattgattc agatggttgt gtcatggata caatgactta taaacacaat
     7021 ctctttttcg ggccaattgc tgcagagatt tttgatgttc atcataggga aaaattccta
     7081 caggattgga ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt
     7141 ttagttaaaa cattagaaaa aaatgatgta ccatcgatta atcgattaaa gcactgggta
     7201 tctgtaacaa aagaattgtc taatccatct ttgaagaggg aaatagaata ctctcagtca
     7261 gaggatttat taaaagcttt aaaatggtcg gaagaagtca atcgacagat tagtcaagct
     7321 aaaggattag ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat
     7381 gggaagataa ttattgtaag ctcagctaat aaggaagcta ttcaagaaga atgggaaaga
     7441 catgaattat tatccttagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagac
     7501 attattcgaa gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat
     7561 tctcctggtg atctagaagc tgcaaataag aataaagttt tcttttatcc tattttggtt
     7621 aataaagaaa aagaatcatg ggaaaactta agaactgata ttttatttaa atgtttatcg
     7681 ggagagtata tagatattga acaaaaatat atagatactt tttggaaaaa tcttactaat
     7741 aagtagagga gaaagaaaat gactataaaa gtagacttac gaaaaaaacc atatcattta
     7801 gatgaagatg ctatttcttg gattgaagag acgattgatg ctatgacttt agaagaaaaa
     7861 ataggtcaat tatttgttaa tatgggatct agtcgtgata aaaattactt aagtagtatg
     7921 gtcaatcagt accatattgg agctgtacga tataatccag gaactgctga tgaggtatat
     7981 gatcaaaact atatcctaca aacagaaagc aaaattcctt tattaattgc tgctaataca
     8041 gaagcaggtg gtaacggagc ttgtacagat ggtaccgaag ttggtgtaga agtaaaaatt
     8101 ggtgcaacaa atgatgcgaa atatgcttat gaattaggga gagtttctgg aattgaagca
     8161 tcggctattg gatgtaactg gagttttgct cctattgtag atattaatag aaattggaga
     8221 aatcctatca tttctagtcg atcttttgga caaagccctg ataaagtttt agaacttagt
     8281 ttagcttata tgaaaggtat tcaagagagt ggaattttgc ctgcagctaa acatttccct
     8341 ggtgacggta ttgacgaacg agatcaacat ctatctttta gtattaatag tttaagtaca
     8401 gaagaatggg ataattcttt tggaaaagta tactcaggct taatagaagc tggattacct
     8461 tcaattatgg caggtcatat ccatatgcca gcttattcaa aacattttaa tcttgatctg
     8521 aaagatgaag atttacttcc tgcaacttta tcaaaagaat taatcactga cttacttaga
     8581 ggaaaactcg ggtttaatgg attagtagtt actgatgcta gtcatatgtt aggaatgact
     8641 ggggcaatga aacggtcaga attattgccc acttctattg cggctggatg tgatctattc
     8701 ctatttttta atgatccaga tgaagacttt aattatatga tggaaggtta ttacaaagga
     8761 attatttcag aagagcgttt acgtgatgca ttaagaagaa ttttaggctt aaaggctaaa
     8821 ctaggtttgc ataaaaaaga aaagaataaa attttgttac cgaaacaaga agctatggaa
     8881 aaaattggtt taattgaaaa taaatcaatt tttaaagagg tagctgatca agcaattaca
     8941 ctagttaaag ataaacaaga tatttggcct attgtaccag aaaaatataa aaggatttta
     9001 ctggtagatg tacatggggt aaaaggtggg tttggtgaag tagttgctgg aaatagacct
     9061 caggccattg atttattgaa acagattttg tcagcaaaag gatttgaagt tgaaaaatac
     9121 atttcacctg aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa aaatatagct
     9181 ggaatatatg ctgctaagag accaatcaaa gatttaactg aaaaatatga cttgattatt
     9241 aatatagcta atgttaaccc aggtacagtt caaagaattg tttggccagc aagtaaaggt
     9301 actccggata ttccatttta tattcatgaa attccaacaa tttttgtatc tgtacagtgt
     9361 ccgtttcatt tagctgatgt gcctcaagta aaaacttata tcaatactta tgatggaaaa
     9421 caagaaactt tagaaatatt agtggacaaa ctaatgggcc tttctgaatt taagggaata
     9481 agtccaatag atgcttattg tgggttaaag gatacattta tttaatattt aaattatata
     9541 ggagtgattt acatgttata tcccattatg acagagacga ggcaagtaat tgatttaagc
     9601 ggtctttgga gatttaagtt agatacaaat gatgacttac aagaagaact tgctttagaa
     9661 ccaatggttg aaaaagagag ttatccaatt tcagttcctt cttcttataa tgatttattt
     9721 gaaacagaga acattagaaa tcatgttgga tgggtgtggt atgaaaaaga agtgattctt
     9781 cctaaaaaat tattagaaga aagattagtt cttcgttttg gatctgcaac acatgaagca
     9841 aaagtatttt tgaatggaaa attgattaat caacacaaag gagggtttac gccatttgaa
     9901 tcagaaatta atcagtttat aaaagatgga agtaacagaa ttacagtggc tgtaaacaat
     9961 gttattgacg atagcacttt acctgtaggg attttgaaag aaacgataca ggaagatgga
    10021 gacataaaaa aagaaaattt ggttaatttt gactttttca attatgcagg cattaataga
    10081 cccgttaaat tgtatacaac tccgaaagac tatataaagg atgtcactat taatacaaaa
    10141 ataattgatt cagatgcaaa tgtagaattt aatatcgata cagaatggac cggagaaatt
    10201 ctaatagcaa tctatgacga agaagctcaa cttatagcag aagctaaggg gaaagaagga
    10261 gtgattcaac ttaagaaccc aacactttgg gagcctctaa atgcttactt atatagtatg
    10321 aaagttagtt taattaacga aaataaagtg attgatacgt acacagaaga atttggcatt
    10381 cgtagtattc aggtttcgaa tgggaagttt ttgattaata ataaaccttt ttactttaag
    10441 ggatttggga agcatgagga cacgtatatt aatggtcgtg gtttgaatga agcagtaaat
    10501 atcaaagatt ttaatttaat gaaatggatc ggtgctaatt catttaggac ttcacattat
    10561 ccttattcag aagaaattat gcgcttagca gatcgagaag gtattgtagt tattgatgaa
    10621 gtacctgcag taggattaca tttgaatttc atggctactt tattagatga caacgtagaa
    10681 aaacatgata cttggaaaga aatacggaca gaaaatgctc acaaaatggc gattaaggaa
    10741 ttgatagaac gcgacaagaa ccatccgagt gtcgtaatgt ggtctattgc aaatgaacca
    10801 gattcggatt ctgaaggcgc taaagaatat tttgaacctt tagttgaatt gactaaagaa
    10861 cttgaccctc agaagagacc tgtaacaatc gttacttact taaagtcaac accagatatt
    10921 tgtaaagtag gagatattgt cgatgtttta tgtttaaatc gctattatgg ttggtatgtt
    10981 tcaggtggag acctaaaaca tgcaaaatat ctgttgaaaa aagagctaga tggatggatg
    11041 gaaagatgtc ccgatacgcc aataatgttt acagaatatg gagcagatac agtagctgga
    11101 cttcatgata cagtaccaac aatgtttaca gaagagtatc aagtgggtta ttacgaagca
    11161 aatcacgaag taatagataa atgtgacaat tttgttggtg aacaagtgtg gaattttgca
    11221 gattttgaaa caagtcaagg gattttaaga gtgcaaggaa ataagaaagg aatctttact
    11281 agagatagaa agcctaaaat gattgctcac tatctaaaga aacgatggtc atcaatacca
    11341 aatttcagtt ataaaaaata atttaccttt tataaggaat ataaaaaagg agtggctgct
    11401 taattgccaa ctcctttttt ttataaaaag attttctgtt taaagaagga tatttttttc
    11461 tagttttttc gaaaaaaagg cgctttctga ttgttgtatt agtggaaagg ggaaaaaagg
    11521 agcatattta tcatgcaaca aaattacaat gagaaatcta tacaacatca atttgatgcc
    11581 tactgtaaaa aagtattacg aaacgaaact aagtctattc gaaaacgaaa cattaacata
    11641 acagataatg aggaaccatt gacgaattca aatgaaggta agtactccca aactcatttt
    11701 gatgaacaag atgtctattt cttatttgga atggaaatat taatttcaga tcaaaagtta
    11761 tcggcagcca ttgatcattt atcggaaata agaagaaagg ttatcctact ttattatttt
    11821 gcaggtttta atgatactga aatcggtaaa atcctcaata tgagtacaag tggtatctgg
    11881 tatcaacgaa agaaggcagt tgaacaatta aaaatggggt atgacttatg gtagacaacc
    11941 ccaaaaaacg gcagtatcca ttagttcctc tatccattat ccaatctgct ctagaagaag
    12001 atccaatttc aatcaagatg atactcaaac attatcatag ttatatagct aagttatgtt
    12061 tgacaaatgg atttaatgag gcggggcaat ttattaccta tgtagatgag tatatgctaa
    12121 gacagctaga aattaaattg atagaagcga tcttaaaatt taaaattaac taactaaaaa
    12181 gtcccctttc cacttattct gtttaaaaga atcttctaat agttaaagga ttagtagatt
    12241 cttttaaaca gaaaacctat tgatcattga aatttaaata cattctatca gtacgttaac
    12301 acttcaagga gcatatttta gttgcgccat gatttcttac tgtaaagaag gagcgataaa
    12361 cattctaaaa taatttttat agtcaataaa aagcgatgat ttgaagttgg gataatgata
    12421 cttccgtaag gctcgccgta aacttggaga gaggtgaaat tcctatgtgc ttttgacaaa
    12481 agcagctgat taagaattat ttccatatgt tgatttcaac attttttcta ttatttttaa
    12541 gaccaaattc agtgaggcaa atagcatcat gtaaatttag tttttctatt gaacgttctc
    12601 cactttttag tttttttata gtactcaaat ttaatcctgt atgtttagag atctcatttg
    12661 cagtatggtt ttgtaaagtt tctcggatta ttgttatatt agcaatttca ttacgcataa
    12721 ttatgttcct ttcttatcaa aaatatcaat gactacagtg ggcttttagt gaaaaaaagg
    12781 ggtactacag tctcttttga aaacaaaaaa atacagtata tacgttttct aaatgctaag
    12841 caccaacgtt tacactgtat ttaagggttt ggcacccaat gggctgtgag gggctcgaac
    12901 ccgcgacccg atgattaaga gtcatctgct ctaccaactg agctaacagc ccaaattaat
    12961 actttgttat tctaacacct aaaataaaaa agtgcaatac ttattatcag aattgagagt
    13021 gaattttatg gattttcaag attatgaaaa aatatttgaa agttatccat cgatattgaa
    13081 aattactgat ttaactaaaa tgttaaatat tggaaaaaat aaagcttata atttgctgaa
    13141 agatagatca atagaaaata taaaaattgg agcaaggtac aaaataccta aaaaagctgt
    13201 cattgagtat atattgaaaa tacaagatga aggtaaaaat catgctataa taaaatagtc
    13261 aaaatttttg ccgatatttc agtttaagag gagagattga attgaaatta agagcaaaca
    13321 taattgaaaa aagaggaaca ttacaagtag ttgtatctta taaagactct aacggaaaaa
    13381 ttagacaaaa gtggaaagat acaaaattaa aaaagaaagg aaataaaaaa cgagcagaaa
    13441 aaattagaga tgaattttta ctagaattgg aaagtcaatt agctatggaa tgtgaatgtg
    13501 ctaatccaaa tttattgttt tatgactatt tgcttaatta tttggaagta tcaaaaaaac
    13561 aagtagcatt taacacatat gttgggtata agcattacgt ccataatcga atttacaagt
    13621 ttttttatcc taaaaaaatt aaactaaata ggttaaagcc ttatcattta caagagtttt
    13681 atcaatatat gttaaatgat aactgtacag caaatacggt tattcattat cacgcattta
    13741 tacgcaaagc tcttcaagag gctgttatta cagaattgat aagtacaaat gtagcagata
    13801 aagtaagaaa gccaaaaaag aaacaatttg tttctcaagt ttataatcaa caagagatct
    13861 tgaaattact tgacattttg tcagaagaaa aactctattt ggtagttctt ttaacagcat
    13921 tttatggatt gcgaagaagt gaagttttag gattaaaatg gtctgctatt gattttttaa
    13981 acaaaaaaat catgataaac catgtaatca tagaaaatcc agagaatatg tcacagttaa
    14041 taaagaaaga tcaaacaaag aactcttcca gctatcgtac attacctttg gttgaaacga
    14101 ttgaacgagc attagtaaat caagctaaat ggcaacaaga taatcgtatt cttttaggag
    14161 atgattatat tttagaagat aatgagtacg tatttactat ggaagatgga cggttgatga
    14221 agcctcaata tgtaacgcat cgtttgagta agttgattaa gaaaaatgga ttgaaaaaaa
    14281 ttcgctttca tgacttgcgt cattctaatg cttcaataat gttagatagt ggccagaata
    14341 tgaaatccat tcaagaatgg ctgggacatg catcgtattc gacaactgct aatttataca
    14401 cgcatttaac cgcaggagta aaagatcgtg cagcagaagc attagaaaac gcatttgggt
    14461 ttaaagaata aaaaaacaaa aaacaatatc tttggggtat cgcttcttgt ttttagaatt
    14521 tttttgttgg caatttgttg gcagtatgtt ggcaatgaaa aaattcacaa actaaaaacc
    14581 tatttataaa gctttataaa acttttttca caaatagagc atttggttaa gagtcagctg
    14641 ctctaccaac tgagctaacg gcccgtacaa aaagtactta ataatagtag cactaagaat
    14701 tttaaaatgc aagactacat ttaaagaaaa tcagaaaaat ctttacttcc ttataggaaa
    14761 tcattcatag attattcaca tttgctcgct attatataag cataccaaca aacaaagttc
    14821 ttgcaagaga acaaacccta acactttttt cattttttac tcctttcccg ccgtctcccc
    14881 ggcgggtctc ctgttcttaa cgataaaaga caagaaggct tagccttctt gttttttgtt
    14941 ttcatggaat cccgctaatc aaatcaccta gttaaagcaa ctagattgaa tgtttatcat
    15001 aagtctgata tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa aaaatgcgtc
    15061 agcaattaat ggagtggggg aaggaactag aaacattccg tctgcctcac tgggaagaac
    15121 tccctgactt agaactttat atggaccaag tcagaacgtt agttgatcgt tatctttcac
    15181 ctgtgataca aggagaaaag catccattgc ttacctcttc tatggtaaac aattacgtta
    15241 aactaggttt gataccagca ccagttaaaa aaagatataa caaagaacac gtagcatttt
    15301 tacttgcaat cactacgttg aaacaagtat tgactattcc tgaaatcaaa gaagggatat
    15361 tgttccaagg aaaaacagta ggaattcgtg aagcctataa tctattttgc gatgaacaag
    15421 aagcagctgt gtggatggtc agccaattag cacaaggtaa atctcatccg caaaaattcg
    15481 accaggcaac ccctgtagaa tatattgctg taagagcggc aactttatcg tttgcaatga
    15541 aacttcttgc ggaaaaaacg attgttctag aaaccgaata tttaaaagag gagaaaacaa
    15601 atgaaaaaca ataaaatcgc aattttagta gattcaggta cggatgttcc agaagaattg
    15661 ataaaaaaat acgagatgta tatgatccca ttgaaaatta tttataaaga tcaggtatat
    15721 acagataaaa ttgacattac ccctgaagag atctatcaac gtttgccaga ggaaatccct
    15781 ggtacatctt tgccggatgg tgagtcgatc acagaaatct ttgagcaaat caaaaaagac
    15841 ggttatgaaa aggtacttgc ggtaacaatt tccagtggat tgagcgggac atataatgtg
    15901 gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat atgtactaga tacgaaaaat
    15961 attggtattg gtgcaggttt ttctgcgatc caagcagcaa aatggctgga agaaggagta
    16021 gaatgggatc aactgatcag taatctgaat gagttagtcg aaagaacaaa agttttcttt
    16081 aatgttgcga cattggaata tcttcaaaaa ggtggacgga tcggtttagt tgcttctatt
    16141 gtaggaaccg ctttaaagct taacccaatc atctcatgta atgaagaagg gatttattac
    16201 acagtaggaa aagctagggg acgtaaaaaa agtttggata aaacggttgc ttacgtcaaa
    16261 gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga tgcgaaagaa
    16321 gaagcaattg aaatggaagc gagacttaaa agagagttcc cacaagcaaa ccaaatctac
    16381 tttggccaga tttctccggc attagtagtc cacactggtc cgggattatt gggtgtcggt
    16441 gtacaagttt tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa tcagtgtttt
    16501 ccttttttat tgaagttaga acaaaaaata ataaagtatt ttgcactttc attttaattt
    16561 tctcttttgt tttataggca aagatgaaac aaaagcagaa atacggtata atgagaaggt
    16621 atgacaataa tatataagag gtggctcttt atgaaaaaag ttttagttgt cgatgatgag
    16681 aagccgattt cggatatcgt caaattcaat ttagccaaag aaggctatga cgtttatact
    16741 gcatatgatg gggaagaagc cttggaaaaa gttgctgagg tagaacctga tttgatttta
    16801 cttgatttga tgctacctaa aatggacggc ttagaagttg ctcgtgaggt acgcaaaact
    16861 tatgatatgc cgatcattat ggtaacagcc aaagattctg aaattgataa agtcctaggc
    16921 ttagagttag gcgccgatga ttacgtgacc aagccatttt ccaaccgaga attagttgca
    16981 cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga aggaaccaga agaggcagca
    17041 cctgcggaat tgacgattgg tgatttgacg atccatccag aagcttacat ggtgacgaaa
    17101 cgaggagaaa caatcgagtt aacacatcgt gaattcgaac tactctttta cttggcgaag
    17161 caccttggac aagtgatgac aagagaacat ttattacaaa cggtttgggg ttatgattat
    17221 tttggagatg tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa gatcgaagat
    17281 aatccaagcc atccaaatta tttagtgaca cgtcgtggtg tcggttacta tttgcgtaac
    17341 ccagaacagg agtaattgga tgaaaagaaa agtccgcttt tttcgatcag tcaattttaa
    17401 gatcgctatt acctttattc tgattttatt gatctcgatc gaaattattg gtgcctactt
    17461 tatacgtggt ttagaacgtt ccacgatcaa tacatttatt aaagatatga atcaaacggt
    17521 ggaatcatta gcaacaacaa tcagcccgga attgaatcga aaagataatg cagatgatga
    17581 agaggtaaat gcgaatatca aaagatttat tgaaaacagt gcgactagcg atatcatcga
    17641 aatacgggtc gttgacgaaa aagggatcat ccgaggaaca accgatgtca atgaacaaag
    17701 cgctgtggga aagaaaaatg attatgttga tatcaatgat ttcacgacta aacgttatgt
    17761 tgctttagat aacgacgata aacgggtgaa tatcaatgtt caaccgatcc tgtccccgac
    17821 cggcgatgcc gttatcgggg ctctttacgt caaaagtaat atcgaacaaa aatattcgga
    17881 aatcaataac acagcagtca ttttctttac tgcctcacta attgcggcat ttatttcaat
    17941 gatcgtatct gttttagttg ctcgttcgat tacacagccg atcggtgaaa tgagagaaca
    18001 agccattcga atcgctagag gagactacag tcggaaagtc aaagtgcatg gacaagatga
    18061 acttggacag ctggctgata ccttcaatca gttagctgaa cgtattgaag aagctcaaga
    18121 tacaatggaa gctgaacgca atcgtttgga tagcgtgcta tcccatatga cagacggcgt
    18181 cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat gatatggctg tttccttgct
    18241 agatatcaaa aacgaggaag caatcggaca atcgattttg acacttttag atatcgaaga
    18301 agaatatacg ttgcggaaat tattagaaga tccaaacgaa atggtacttg accgttcaac
    18361 cagtacattg gaaagtgatc agatgatctt gcgagtagac ttttctatga tccgtcgcga
    18421 atcaggtttt atcagcggat tagtagctgt cttgcatgat gtgacagaac aagaaaaaac
    18481 agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta caccgttgac
    18541 aagtatgcgt agttatatcg aagcattgag cgaaggtgcg tggaaagacg aggaagtagc
    18601 accaaatttt ttgaaggtaa cgctagatga aacagaccga atgattcgaa tgatcaatga
    18661 cctcttaaat ctatctcgca tggatacagg taatacccag cttcaattag aatatgtcaa
    18721 tttcaacgag ttagtgaatt ttgttcttga ccggtttgat atgatggtgg gaaatcagga
    18781 aaaaaattat aagatccgtc gtgaatttac gcaaagagat ctttgggtcg aagtagatac
    18841 agataaaatc atccaagtag tcgataatat catgaataac gcgatcaagt attcaccaga
    18901 tgggggaacg attacttgtc gcttattaga aacgcataat aacgtgattt taagtatcac
    18961 cgatcaagga ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc ggttctatcg
    19021 agtagataaa gcacgtgcaa gagcacaagg tgggacagga cttggattag ctatttctag
    19081 agaagtgatt aaggcgcatc gaggcgctat ttgggcagag agcaaggaag gaaaagggtc
    19141 gactttttat atctcgctgc catatgaacc ttatgaggag gaatggtggg aatgagaatc
    19201 tctgaaaaag tcgttcgtat aggactcatt ttgatggttg cgttaagcat atacttctct
    19261 tatgcaatct ggctgagccc agcaggaaaa agttcaatca acgtagatga gacaaacact
    19321 caaatgatcg agtctcaaag caatcgaaaa acttccgaag tctttcttcc gcttcatgtt
    19381 acatggcacc attcgggaat gatccaagaa accaacagtg aaaatctcgt gtaccgattg
    19441 caattgatca tcgataaagc aagatttgga aagctttctg aagtagtttc aggagacaaa
    19501 tcacagtttg aacaggtgaa aaatctgaac tctggttttg agttggccta taatgcgcct
    19561 tttttgctaa gagaatacaa ggaagcattt gatttagatt tagatgtgtc gaacttggca
    19621 aaagaaagcg aaaatatgta ttttactaga atacaattcg atcaaggaaa taaaaaaatc
    19681 cgatttttga attattcgaa ccatctcgta tatgaagcaa atatttcgtt gaattggaca
    19741 gatatcaata aagaattaac agcaaaagac acaaaatgga cagaaatgac aacagaacca
    19801 tcgacgatcg acacgcaata tgctacgaaa cattcgatca aactgaagaa gtatagttat
    19861 atcctgtcac agcaacccta tacacttttt agaaatgctt ttttccagaa accagatagc
    19921 gtgaaaaata atgatgaaag caatgaattg attttttacg gtgggaatga aacaatgtct
    19981 atccattcgg aaacgcaagt agttgatttt cgtggagagc tgccagagaa aaaagaaaat
    20041 gctagtgtat tttctgaaag tttcccctat attagcaaat taggcagttc tttagggaac
    20101 ctcagatatt ttgaccgcag tgacaatacg atcgattatc ggatattcgt ggaaggcttc
    20161 ccggtttttg ggacagacag cagaggacag attggtgtgg aagttggcgg tgaaacgaat
    20221 ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc gataccttca
    20281 gacgaagaag tagaacttgc aagtacgaat gaaatcatcc agcagttaaa agctaaggga
    20341 gcagatagca acaagattgg ttcagtgatc gttggctata catggcaaaa tgtcaaagaa
    20401 acgaatcgtg tcgttgatct cttacctgaa tggtatgtga aatataaaga taactggtat
    20461 tcagctaacg accttctgga aaaattacct ggattggagg cgaattaatt ggatttcaaa
    20521 aaaatcgaat ggatattttt cgtcgctttt ttagggttga atgtgttttt attcagcagt
    20581 tatcatgacg cggtatacca agaaaacaat gtcattcgct ctaatcagac tgaatcaatc
    20641 caacaacgtc tggctagcga cgatatcact tacacgggga agatttcgtc tgagaacaag
    20701 caaggctatt atcttagcgc ggagcagacc aatcttagca cttcattagc gaattatcgc
    20761 aaggctggta aaagcggctt gctggcaagt ggtgtgtttt tagacggtga tgttttgacg
    20821 aaatctttat ccagttcaga tgctgaaaag aacgtgatcg atccagataa aattagtagt
    20881 tccttggaag attttttatc taacaaagac aatattttat tcggtaatga ttatgtgtac
    20941 acgaaaaact tttctcattt agaagaagaa ccactagaaa tccaagcaag ccaagagtat
    21001 gaaggcatcc cattcaatga tgatacagca aaaatcgttc tttctttaga aaaaaacgat
    21061 aatgattata gcatcacgaa atacatgcag acgcatttat ctgatatcga gcagctgcgc
    21121 gaaaagacgg aactgtatac ggaagaggaa gcaatcaata cattgtacat caataacaaa
    21181 ataccaagag gatcaaaaat tttatggcgt cagctagctt attcctgtat tctgaaagtc
    21241 cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag cgataaagtg
    21301 gtccaaattg agagcgtcaa cgctttcagc aatacgatcg tgacaaacaa tactgtacca
    21361 aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag taagaaggga
    21421 agtaggagtc aaatggctga agaaaaacca gcttttaaaa tcagtatcct agcaagtgga
    21481 agttcgggta attcacttta tatcgaaagt gaaaaaaaac ggcttttggt cgatgcagga
    21541 cttagcggta aaaaaatcac ttccttgatg gctcaaattg atcggacacc ggaccaatta
    21601 gatggaatac ttgtgacgca tgaacatcga gatcatatac atggcgtggg tgtcttggct
    21661 cgtaagtatc atttggatgt ttttgccaat cagcagactt gggaagcgat ggaaccactg
    21721 attggaaatg taccagtgga acaaaaacat ctatttgaga tggggaaagt cagaacattc
    21781 ggcgatatcg atattgagag tttcggcgtc tcccatgacg cagcggctcc gcaattttat
    21841 cgtttccata aagaaaatcg gtcattcgtc atgttgacgg acaccggata ttgcagtgat
    21901 tatatgcgag gtgtcattga aaatgccgat ggttatttga tggaaagtaa tcatgatctt
    21961 gagatgttac gcatgggacc ttatccgtgg aatttaaaac agcgtatttt aggagataga
    22021 ggacatcttt ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg taatcggacc
    22081 aaacgaatct atcttggcca tcttagcaag gaaaacaata tgaaagaact tgctcattta
    22141 acaatggaaa atgttttgaa agaaaaagat tttggcgttg gacatgattt tgaaatctat
    22201 gatacagatc cagatgaagc aactgagcta ttttcgattt aaacagttca atcaataaaa
    22261 atataaaaag cagaagataa aatcaatcag ggttggtttt atcttctatt tttatttttt
    22321 tagcatatta aaaaaataac cgtattatcg actgactaca ctgatgtgct gcttttcatg
    22381 tttctcatcc agtatctcat ctactaatgc aatcgcgtaa tctgccatgc tgatcgtact
    22441 gtcgctagca gcgtttcttt gtaaataatc atcacttaac aaataagtac ctgtacgttt
    22501 gccatgtgga tcaaataaag aagctggact gatataagtc caatgtagat ttttagattg
    22561 tttcaatttt aacaatcctt cgtacatatt gaaagctgtt ggatagaagg gtgcattttt
    22621 aggtacttgg tcaatcatac gagtcgtctt ttctgtatct aagaataacg aagaggcacc
    22681 cccaacgaca attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat gggacaaaga
    22741 agtttgatgc atttcttctt ttcctttggg agcgttgaaa gcatcaacga caacatcaaa
    22801 agatgcaaga tcagtagttg tcaactcaaa taaatccttt tgtataaacg gaacatctga
    22861 tggcaatttt tctggatgac gcacgattgc cgtcacgtct aaatctctgg acaatgcttc
    22921 gtctaaaata aatgaacctg catggccagt agctccaatg agtgcaattt tcataaaaaa
    22981 cctccttttt tattcctgtc cattgtaagc agttgctgaa tattcgtcaa agaatccgat
    23041 ttttgaaaag ctcagtgcgc attcctttca tggttcgtga tcattgggta tagttaaatt
    23101 aagaaaactg attgataaaa atagaagttg aaggagataa gaaatgaaag agtggttaga
    23161 tcgtgccttt ttaccaatga taacatctgt tgtgaatctt agacccttaa ctgctttgcg
    23221 taatggaatg acttacacga tcccaataat tattgttggc tcgctgtttt tcttatttgc
    23281 tcattttcct gtggattaca tcgctcagtg gatagacaaa acaggatttt ctatgtattt
    23341 tacacaagta taccaggcta catttgatgt gatagccttt tggatcgttt ttgccattgc
    23401 atacgcttat gcaaaagaag aagacatcga tcgcttacca gtcggcataa cagccttgtc
    23461 tgcttatcta ctgcttattc agccactaaa tactcagata gatactgtct ggacaggtgc
    23521 tgaagggata ctaggagcag ttacaactgg gctcatcatc ggatggggct atgcacgttt
    23581 agtaagactt gcgaaagggt ggaagcaaag agagaagata cctgaaaatg tttggcagtc
    23641 ttttacttca ttgattccaa tcattgttat agtcactttt acgcttatcg gttcagcggt
    23701 ttttcaacat ttgttcagaa tgactgttgt tcaaggagta tggacatatg tccagcttcc
    23761 cattcaatcg ttcatagaca cgttgtttgg tgtgctttta ttaggttttt ttgttccgtt
    23821 tttatggtta tttggtcttc atggatctgc gatggtcaat gggttggtta gtcccgtctt
    23881 gcaagcgaat tcattagcaa atgcagaaat cttagcctca ggacaagagt tgacggtggc
    23941 aaatggcgga catatcgtta ctcagcaatt tctcgatcag ttcatgacgg ttacgggtgc
    24001 tggattgacg ttaggggctg tattttttat gacggttttt gcgaaatcga agaaatacag
    24061 agaattagga aatcttgctc ttttaccagc ctttttcaac atcaatgaat caattatttt
    24121 ttccacaccg atcgtgatga acccgatgat ggctgttcct tttattttcg caccggtcgt
    24181 ttcaggattg attacctatt ccgctttata ttttggattc gttcctttat ttacagcagt
    24241 ccaagtccct tggacaacac cgccgatcct ctcgggtttc ttagtaggcg gagtgcctgc
    24301 agcgatttta caaggtatcg tgttgtcaat tagttttttc atttatttcc catttttgaa
    24361 aaaaatagat tctgcaaact gcaaaaagga aagacagaca aaaaatgatg gagcagcaga
    24421 aaagatgaac gactaaaaaa caaaaatact cctgtcgctt tagattggga aaatcaccaa
    24481 tctaggatga caggagtatt tatagtcatg tttatactag ccctttttgg acagctaatt
    24541 gaacaagaac aactaatgtg ttcattccta cgtgtgtgat catcgatgtc cagatccgac
    24601 cagttgcttt gtactctagg ctgaagaaga aacctaggaa aaagtagatt agcagatgac
    24661 catcattatg agcaaacgca aacagcaaag agctgatcac tgctccgacc caaaaatttg
    24721 aatatcgact gatgatcccc agtatactgc ggcggaatac gaactcttcc atgatgggtc
    24781 cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg atgatatttt
    24841 gtgtattctc ggaaggggtt gctttgccaa acaatacaga ttctaacatg acagcgatgc
    24901 tttgcagaat caatgcgaca aagattccaa ccaatccata aataacaatc tttggagcag
    24961 aagctttttt taccgctgat tcgatcgcta agggttcacg ttgcttaaaa taaaaaatag
    25021 ctaggacaac ggcgcctagt atatacatga ctgcagttgc tgtgatcaat tcagacgtag
    25081 attttacaag tcctatcgaa gctaggatca agggtgagat aaaaattaga ccatagcaaa
    25141 aaatgctgag gaagctatat ttttttaaag acatgtaatt cctcctttta agggataaca
    25201 agcatgcaag gcaagcttat atcgattgat ttcaattata tcataatttc aataagcagc
    25261 tgtcagaata aaatttcttt tctttgattt aaaagcttgc aaaaaagagg gagaatgagt
    25321 attattaaaa atgtaggtta gcactcgaag gttaagagtg ctaacagatt aaaaaaagag
    25381 ttatcggagg gatttatcgt gttaaaacca ttaggtgatc gcgtcattat cgaagtcgca
    25441 aaagaagaag aaaaaactgt cggaggaatc gttttagctt ctgctgctaa agagaaaccc
    25501 caaactggga cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg tgaaaaagtt
    25561 cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg tacagaagta
    25621 aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc gattgttgag
    25681 taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca atcatggcaa
    25741 aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta gataaattag
    25801 cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta gaaaaatctt
    25861 acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc gaattagaag
    25921 atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa acaaatgata
    25981 ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt cgcgaaggtt
    26041 taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc gaattggcaa
    26101 caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca aaagaagcca
    26161 ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg attgctgatg
    26221 caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa gggatcgaaa
    26281 cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca caatatatgg
    26341 taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg atcacagaca
    26401 aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg caacaatcac
    26461 gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact cttgtattga
    26521 acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc ggcgatcgtc
    26581 gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt actgacgatc
    26641 taggtttaga attgaaagat gcaacaatcg aaaacttagg aaatgcttct aaagttgttg
    26701 tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca atcgaagctc
    26761 gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac cgcgaaaaat
    26821 tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt ggtgcagcta
    26881 ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat gctacacgtg
    26941 ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac gttatcagca
    27001 aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc gttgttcgtg
    27061 ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga tcagtgattg
    27121 ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact ggagaatggg
    27181 taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct gccttgcaaa
    27241 atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca gataaaccag
    27301 aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggt atgatgtagt
    27361 aattaagaaa gaaccctatt ttaacagggt tctttcttct ttttttgttt tattttaagc
    27421 aaaaggggca aagaaggggc aaaaatatca aaggctattt agtttatcaa ttacttgttg
    27481 tttggctctt tttgttacat ggttataaat agacagagtt gtgtttgcat cagaatgccc
    27541 tacacgctcc ataatggctt taagaggtac gcctaattca gataatagtg aaacgtggct
    27601 atgtctgaat atatgtgagg atagactttt ttctaattcc aactcttcct ctactttatg
    27661 gagtatagcg ttaaatgagt gcagcgcaag cggagtgcca cttgtagata taaatatata
    27721 ttgatcggga tctgtgggtc tacctgcaag aatattgtca gctatcacac tttcaatcaa
    27781 ttcttttgca cgattgggta attgcacttc acgttgcgaa taagtatttt ttggagttgt
    27841 ttttatagca ttatccattt tcacagatgt gtaatctaag gtcccattaa tggaaatttt
    27901 cccatcctca tagtccttca tttgcaaagc tagcaattcc ccatatctca aaccagttaa
    27961 atatagaaac tcagctatta tgccgtgtag ttttctgcga ggattggagt atagctgttt
    28021 cagtatttga tcaatttctt ctttctccag atatttttta ttcatagaaa gccttctttt
    28081 ttcttcttcc acttttttag gatggatttt aactgctagc gcagggtttc tttgaatgta
    28141 ttttctatcg attgcatagt ttagcataac agatagagtt gtttttgttt gttttgtgta
    28201 gttcagtgag aggtcaccaa acgtatacat atcttcaatt atcttattaa tgagtgtctc
    28261 atcaatgttt ctaacgatcg tatcatcgct tatgtgctta gaaacatgtt tcatcatcat
    28321 tggaaccttc aaatagctag tacgtttaac atgctgctta taatattcat accattcttt
    28381 atacagctca ccaaaagtga tatctgattt attgtaatct tcgagtgctt ctttaatttt
    28441 tttatctaga attttctgag cttttttcca cgcttgtggt gaattacttg taagtgttgt
    28501 agattttttt cgtgtttttt ctgtataagg atctacatat ctttcaataa acttgaattg
    28561 gccatctttg gtctgttcaa cccacacttt taacatctcc tatcatttgc tataataggc
    28621 ataacaaata gacctatata ggtttgtttt ctaaaagcac gctcttactc tggacggtgg
    28681 ggcgtgtttt ttatttatta tacagttgct ttaaaaatag ctgctttttc aaatgatact
    28741 aaaggagaga agtggatttc tatttctccc ttttggttta acgtgaaatg agcagttaca
    28801 tccatttgtt ttcctggagc aacagatccc attgtatttt cgttggcata tgtctctgat
    28861 tttttgtcat ctggtccata tacttccaca tctgtaccta cagggatatc tgaatcgcca
    28921 tcatttttta cggtataagt aatttttact acttgtgcag gttggttttc ttcaaattga
    28981 tttctttcat cagttagttc tacactattt agtgtatatt cagcatcacc aacttttaca
    29041 gtgtcaccaa tcttatagaa ggtatcgctt ttttcttctt tagaagatga agcagaggtt
    29101 gaagatttag ttactttttc gccaccatta tcacttgctt tattactatc tgatccacca
    29161 tttaaagcag aaccaataat tataattaaa attactgcta atacccaaaa ccatactttt
    29221 ttgtaaaaag gtttactaac tttatacatt tttccgtctt gacccataac tttttttgcc
    29281 atttaaatat tcctcacttc ttgttataat atatttgcga tctcagaaat gaggtatgag
    29341 tccgtgtgcc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    29401 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    29461 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    29521 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    29581 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    29641 aaaactgaat attcaaaatg agaagcggtg aaatgcgcaa gttcatgaga taaatgaaag
    29701 gcttctgcag tttcgccata tagattttca ttcaaaaaaa tgattctagg cttcggataa
    29761 tagaagccag gctctttcat ttccatgtaa actactttca aattgtattc gctcaacatt
    29821 tctttcaatt tcaaatacat acaacccatc actccaacta ttcattttcc tctaaagctt
    29881 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    29941 aaacagtatc gttttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    30001 gaaggtagtc tacagagact ccaaagtaat cagctaaccg agctaactca atagaattag
    30061 gagtttgtct tttccaactt cctaaatatc cattcgaata cccgaaattc atttcgagct
    30121 gtctaatgga taatcctttt tcttttgcta actcctttat tatttcgtaa gtattcattg
    30181 ataaatcaac ctttctgaat gcttacaaaa aaagtttaga taaataatgt aaaaatagtt
    30241 gacttatata cattattaat ctatactatg ttttgtaaac aagtttaaca actaaaaaga
    30301 caacaaaaaa tactattgat taataaatgc caaccgccaa gaaagctttt taaatcaaag
    30361 tttatatgtc ttatttaact atgcatttag tatagattat taatctaata aagtcaacgc
    30421 agttttttaa aaagttgtta aatttgttta cgaatataaa agaaaggaga gaagaatatg
    30481 gaaaaaacag tctcggcaaa aatcgaagat ttgaaattag acatactgaa acaagcaaaa
    30541 gtggcgatgg aacacgcggt agataaagaa gactctgcca tggttgcagc catagcagag
    30601 attttagctc acgtttagtc atcgttttta cggtcttctg gaagctgacc atagataaca
    30661 gcataatgaa tatctaaata agcttgaaca atttcttttg gcgaaattga ttcgccttta
    30721 gtagatactt gtgattcgtg atttagccat gcaacaacta aatccgcagc gatttcaaac
    30781 ggcgttacac tttgtgtcag ttgccataaa gaatttcaca atatgtatgg tcgtggtaat
    30841 aataccaaag agcaatttga ggaatttatg gaaattaaaa aatacaaaaa atcatttatt
    30901 aaaggaggct aaacaatgaa caaggaaatg agccaaacaa tcaaggtaca aaaaatgata
    30961 gatgatctaa cacatggaat tgatagccaa gccgataaaa tcatcaagga attgcaagga
    31021 caaaaagtta aggatgcaaa aatgcttctc aagactatca actttgaaat gaatccaaca
    31081 aaaagaaagc tcgccgatgt gttggaagaa aagttggctt ccgctatcaa cgagcaagaa
    31141 ttactatttg aaaccgatac ttttaacagc tgatgtttta tagatgacag tttcatccga
    31201 atctatatca tcgccgagag aaaagaatgt tgcttttgac aaaaagccga taagacctac
    31261 aagcggtctt gatgttgcaa gagtagatac cccaatatca ccttgcaagg tattcacgta
    31321 aatttgagaa gaatagaaca ttccatcttt ttcttcgata tttgacgcat catcgtatcc
    31381 aacgaccaaa gtatcggcaa atattttgat tttttctcca tcaagcatag taattacaaa
    31441 ttctggtttc ataaattttc acctccttaa caattatttc agcctgtcac actgataagg
    31501 aaattatacc aaagaaagga atgagaaata tgaatacacc acaaattttt aatttcgagc
    31561 aaaacgaagt tcgaactatt ttagtaaatg atgaaccata ttttgtagga aaagatgtag
    31621 cagacgttct aggatattcg aatccgcaaa aagctattcg agatcatgtg gacttggagg
    31681 ataagacgca gaacgattcg ttcaccgtca atggaacagc agttgttttg attaacgagt
    31741 caggccttta cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt
    31801 gggtaacgag tgaagtcctt ccaacaatta gaaaaacagg tagctattca aacgtacctc
    31861 aaagttttgc acaagcattg cgtttagcag cggatttaga agaaaagaac caattactcg
    31921 aacaacaaat tgccgagtac gaaccaaaga ttagctactt agatacgatt ctttcatcga
    31981 cagatacggt agctacttct caaattgcag ctgattacgg aatgtcggca attgctctaa
    32041 acaaattgct aaacgagtta ggtgttcagc ataaagttag cggacaatgg atactttacc
    32101 gaaaacatat gaaccaagga tacacaaaat cgcacacaag tgagataccg aaagccgatg
    32161 gcggcactaa agttgtaatg aataccaaat ggacacagaa agggcgagtg tttatttaca
    32221 acttattaat cgcagagggc tattaccctc aaatggattt agaggaaatt ggttagaaag
    32281 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    32341 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    32401 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    32461 tatagattcg attatcacta tgtacgaatt gtagagggag gtaaagtcgt tggaagataa
    32521 aatcattctt acaaaatcag agcttcaaga aatgttaaac atcgagtatg gaacgataat
    32581 ttttaggagg gataataagt gaaaaaacca acactttcag agctgataga agctgctgag
    32641 aaggcagtaa aaccagacga ctggtaccga caaagtttaa tcttggagaa gttccacggc
    32701 atgtcaaaaa ctactttagt tgaatactgc aaggaaatgg aaacaattcc tgaatttgca
    32761 gaaggaatac ttcgtccagg tcactcaacc acatttattc attaccatac ttttatttgg
    32821 tttttaaagt ggaaagacgc aaacaaatat cgtgtaaaaa gtttatcgcc ttcagatgtt
    32881 ttgaaggagg caagttaatg ggcaaattca acagagcact agtattcagc gcaccgctaa
    32941 tcatctacgc tttaggcctc tggggaagca gacaagcgtt gatagggaca atcgtttaca
    33001 tggtttggat ttttatgggg cttgatgaag ctgagcacag agcgaaaaaa gccagtcggg
    33061 agggactgac taaaatatga tttttttatt tttgttcctt tgggctgcta ttgcgtactt
    33121 catccagctc cttgttgtca aagttgctct tatttctttt aggaagaaca aaagaaaata
    33181 gcgctataaa tgaaagataa gtagccgcta cattaaactg ttcttttgaa aaagttacag
    33241 tcatcggaat gataattgat attgaggaag taatcttaat taaaaatata gcaatatttt
    33301 caacatcgcg ttggttttta tttaacattg tccaacatgt tttagcaaca ataaaaataa
    33361 ttacacatga tattagcaca cagactgaaa aaagttttag aaatgcgatg tagtcagtgt
    33421 tattgaactc cttcaaaata ctatttttat tcaatgtaat aaaaggaatt tttttaattg
    33481 aagacaggaa agataaggta ataaacagaa ttatatatat ggttaaagtc gcagtaagaa
    33541 tgtaattgag ctttttcctc atagaatcac ctcactttct aacgaaaatt ataccaaaaa
    33601 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtaactacga
    33661 gcttacaagt ggcagaaagt ttcaaaaaga aacataagca tgtactagaa gcaattgaat
    33721 caataaaaag atcggtcgaa aattcggcca atgttgaaga tgggtccaat tttggacaga
    33781 tgtttgtgga agggaacgag ccagactcat acggaagaag tcggagagtt tatttcatga
    33841 atagagacgg attttccttg ctagctatgg gattcactgg aagtaaagca ataaatttca
    33901 aactaaaatt tattgaagct ttcaacgaaa tggaagatgt tattcggaag aatactgttc
    33961 ctcaaacaat tgaagacatg atgatctatc aattagaaga aatgaaagat gttaaaaaag
    34021 atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc
    34081 aaaaaggaaa tatgaaagtt atggaagttc tagggggaaa agaaagccga gcttatgaag
    34141 aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc
    34201 caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt
    34261 ggttaccaga aactgcgatc cgcatggaaa tcgatcaact gaacagacaa caaagacttt
    34321 tcggtgatga aaatgaatag agctgaagcg ctaagaatag ggacggtaat tgctaatcgc
    34381 tggtggagac acaataaacc aaacatccta agccaacaac atatcgaaaa gcaaaaagca
    34441 tggcaacaaa taaaaaagtg actcagccgg caagcataga gtcacaaaac atatctaagg
    34501 agaattttag catatgaata aagaactttc cactttagat caatatttga ctgattctga
    34561 atggggcaag tcgaatatca aggaaacaaa taatcgaaaa atcagacgaa atcttttgac
    34621 gaacgaagaa ctatcatgtg atcaagatga cttgggaaat tttgtgagta tttgggatca
    34681 tgtttacctt atccatctat caaaacattc aaataaacct gaatacatct atgtcatcga
    34741 agatggcttg attgatgcgc tagaagagta caacagagat aacttgattg atatctctta
    34801 ttacggatca ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca
    34861 aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa
    34921 aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc
    34981 cacaggcaac ggtaactatt aatgagtata aacactacag ggttgtttct ggaactcatc
    35041 aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaacaggg acttatgtat
    35101 ctgtctcagt aacggttaaa ggacatacgg aaacaga
//
LOCUS       DANJJW010000017        34661 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000017 DANJJW010000000
VERSION     DANJJW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="PWS82_000814"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="PWS82_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL0905961.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="PWS82_000815"
     CDS             complement(2021..2923)
                     /locus_tag="PWS82_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0905962.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="PWS82_000816"
     CDS             complement(2920..3612)
                     /locus_tag="PWS82_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL0905963.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="PWS82_000817"
     CDS             complement(3655..4296)
                     /locus_tag="PWS82_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0905964.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4603)
                     /locus_tag="PWS82_000818"
     CDS             complement(4334..4603)
                     /locus_tag="PWS82_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905965.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4576..5265)
                     /locus_tag="PWS82_000819"
     CDS             complement(4576..5265)
                     /locus_tag="PWS82_000819"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL0905966.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="PWS82_000820"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="PWS82_000820"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL0905967.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="PWS82_000821"
     CDS             complement(5985..6917)
                     /locus_tag="PWS82_000821"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL0905968.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="PWS82_000822"
     CDS             complement(6914..7690)
                     /locus_tag="PWS82_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL0905969.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="PWS82_000823"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="PWS82_000823"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL0905970.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /gene="carA"
                     /locus_tag="PWS82_000824"
     CDS             complement(10934..12010)
                     /gene="carA"
                     /locus_tag="PWS82_000824"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL0905971.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="PWS82_000825"
     CDS             complement(12015..13295)
                     /locus_tag="PWS82_000825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL0905972.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="PWS82_000826"
     CDS             complement(13314..14240)
                     /locus_tag="PWS82_000826"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL0905973.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="PWS82_000827"
     CDS             complement(14253..15536)
                     /locus_tag="PWS82_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL0905974.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="PWS82_000828"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="PWS82_000828"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL0905975.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="PWS82_000829"
     CDS             complement(16324..17235)
                     /locus_tag="PWS82_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0905976.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /locus_tag="PWS82_000830"
     CDS             complement(17232..17696)
                     /locus_tag="PWS82_000830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL0905977.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="PWS82_000831"
     CDS             complement(17701..18399)
                     /locus_tag="PWS82_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0905978.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="PWS82_000832"
     CDS             complement(18386..18772)
                     /locus_tag="PWS82_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905979.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="PWS82_000833"
     CDS             complement(18919..20589)
                     /locus_tag="PWS82_000833"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL0905980.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="PWS82_000834"
     CDS             20772..21005
                     /locus_tag="PWS82_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL0905981.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="PWS82_000835"
     CDS             complement(21104..21535)
                     /locus_tag="PWS82_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL0905982.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="PWS82_000836"
     CDS             21614..22027
                     /locus_tag="PWS82_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL0905983.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="PWS82_000837"
     CDS             complement(22065..23213)
                     /locus_tag="PWS82_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0905984.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="PWS82_000838"
     CDS             complement(23335..23931)
                     /locus_tag="PWS82_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0905985.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="PWS82_000839"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="PWS82_000839"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL0905986.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="PWS82_000840"
     CDS             27161..27592
                     /locus_tag="PWS82_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0905987.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="PWS82_000841"
     CDS             complement(27637..28986)
                     /locus_tag="PWS82_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0905988.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="PWS82_000842"
     CDS             complement(29056..30219)
                     /locus_tag="PWS82_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0905989.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="PWS82_000843"
     CDS             30348..30671
                     /locus_tag="PWS82_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905990.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="PWS82_000844"
     CDS             complement(30854..32203)
                     /locus_tag="PWS82_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0905991.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="PWS82_000845"
     CDS             complement(32272..33225)
                     /locus_tag="PWS82_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL0905992.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="PWS82_000846"
     CDS             complement(33327..34517)
                     /locus_tag="PWS82_000846"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL0905993.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9847 a         7592 c         6088 g        11134 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttcaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa tggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta t
//
LOCUS       DANJJW010000018        34430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000018 DANJJW010000000
VERSION     DANJJW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..673
                     /locus_tag="PWS82_000847"
     CDS             <1..673
                     /locus_tag="PWS82_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL0905994.1"
                     /translation="VSKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLD
                     FFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDA
                     ESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQP
                     TELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYIS
                     GYLDK"
     gene            740..1234
                     /locus_tag="PWS82_000848"
     CDS             740..1234
                     /locus_tag="PWS82_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0905995.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1290..1928
                     /locus_tag="PWS82_000849"
     CDS             1290..1928
                     /locus_tag="PWS82_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905996.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2000..2395
                     /locus_tag="PWS82_000850"
     CDS             2000..2395
                     /locus_tag="PWS82_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0905997.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2379..4832
                     /locus_tag="PWS82_000851"
     CDS             2379..4832
                     /locus_tag="PWS82_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0905998.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4829..6856
                     /locus_tag="PWS82_000852"
     CDS             4829..6856
                     /locus_tag="PWS82_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0905999.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6853..7875
                     /locus_tag="PWS82_000853"
     CDS             6853..7875
                     /locus_tag="PWS82_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0906000.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7892..8821
                     /locus_tag="PWS82_000854"
     CDS             7892..8821
                     /locus_tag="PWS82_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0906001.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9066..9182
                     /locus_tag="PWS82_000855"
     CDS             9066..9182
                     /locus_tag="PWS82_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL0906002.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9198..10001
                     /locus_tag="PWS82_000856"
     CDS             9198..10001
                     /locus_tag="PWS82_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0906003.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10236..10472
                     /locus_tag="PWS82_000857"
     CDS             10236..10472
                     /locus_tag="PWS82_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906004.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10479..12431
                     /locus_tag="PWS82_000858"
     CDS             10479..12431
                     /locus_tag="PWS82_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0906005.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12503..13000)
                     /gene="dfrG"
                     /locus_tag="PWS82_000859"
     CDS             complement(12503..13000)
                     /gene="dfrG"
                     /locus_tag="PWS82_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0906006.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            13362..14402
                     /locus_tag="PWS82_000860"
     CDS             13362..14402
                     /locus_tag="PWS82_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0906007.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            14500..14688
                     /locus_tag="PWS82_000861"
     CDS             14500..14688
                     /locus_tag="PWS82_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL0906008.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14746..15099)
                     /locus_tag="PWS82_000862"
     CDS             complement(14746..15099)
                     /locus_tag="PWS82_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906009.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15630..16052
                     /locus_tag="PWS82_000863"
     CDS             15630..16052
                     /locus_tag="PWS82_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0906010.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            16049..16279
                     /locus_tag="PWS82_000864"
     CDS             16049..16279
                     /locus_tag="PWS82_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906011.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16777..16977
                     /locus_tag="PWS82_000865"
     CDS             16777..16977
                     /locus_tag="PWS82_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0906012.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            17004..18197
                     /locus_tag="PWS82_000866"
     CDS             17004..18197
                     /locus_tag="PWS82_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0906013.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(18265..19830)
                     /gene="guaA"
                     /locus_tag="PWS82_000867"
     CDS             complement(18265..19830)
                     /gene="guaA"
                     /locus_tag="PWS82_000867"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0906014.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(19889..20812)
                     /locus_tag="PWS82_000868"
     CDS             complement(19889..20812)
                     /locus_tag="PWS82_000868"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL0906015.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            21008..21613
                     /locus_tag="PWS82_000869"
     CDS             21008..21613
                     /locus_tag="PWS82_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0906016.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            21921..22937
                     /locus_tag="PWS82_000870"
     CDS             21921..22937
                     /locus_tag="PWS82_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0906017.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            23078..24379
                     /locus_tag="PWS82_000871"
     CDS             23078..24379
                     /locus_tag="PWS82_000871"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL0906018.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24425..25087
                     /gene="deoC"
                     /locus_tag="PWS82_000872"
     CDS             24425..25087
                     /gene="deoC"
                     /locus_tag="PWS82_000872"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL0906019.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            25108..25506
                     /locus_tag="PWS82_000873"
     CDS             25108..25506
                     /locus_tag="PWS82_000873"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL0906020.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25572..26654
                     /locus_tag="PWS82_000874"
     CDS             25572..26654
                     /locus_tag="PWS82_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0906021.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            26866..28428
                     /locus_tag="PWS82_000875"
     CDS             26866..28428
                     /locus_tag="PWS82_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906022.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28421..29554
                     /locus_tag="PWS82_000876"
     CDS             28421..29554
                     /locus_tag="PWS82_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906023.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29554..30510
                     /locus_tag="PWS82_000877"
     CDS             29554..30510
                     /locus_tag="PWS82_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906024.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30609..31775
                     /gene="deoB"
                     /locus_tag="PWS82_000878"
     CDS             30609..31775
                     /gene="deoB"
                     /locus_tag="PWS82_000878"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL0906025.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            31799..32617
                     /locus_tag="PWS82_000879"
     CDS             31799..32617
                     /locus_tag="PWS82_000879"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0906026.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32634..33341
                     /gene="deoD"
                     /locus_tag="PWS82_000880"
     CDS             32634..33341
                     /gene="deoD"
                     /locus_tag="PWS82_000880"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0906027.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(33639..34325)
                     /gene="gpmA"
                     /locus_tag="PWS82_000881"
     CDS             complement(33639..34325)
                     /gene="gpmA"
                     /locus_tag="PWS82_000881"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0906028.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT        11301 a         5988 c         7079 g        10062 t
ORIGIN      
        1 tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt ttacttatta
       61 taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa gagttccttt
      121 tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag caagctccat
      181 atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac ttccagagaa
      241 ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac aagctattga
      301 agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca gagttcacac
      361 tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag ttcctagaga
      421 cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag aaattcaacc
      481 tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa tgattagctc
      541 tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata ttattggaaa
      601 gagagaagca aaactaataa taggtatttc aagtacagtt acagattata ttagcggata
      661 ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt ttataaaaag
      721 gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg
      781 tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg cagaaagaat
      841 cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc cttttgatgt
      901 ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga
      961 aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta gtagcttgga
     1021 agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt cgatggaaga
     1081 tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt ccaatttaca
     1141 aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta attacctagt
     1201 tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt tataattaaa
     1261 ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat cttaaactat
     1321 taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt cgttacaaac
     1381 agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg
     1441 ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta gctgtctatg
     1501 ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt ggtatcgaat
     1561 acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct cgatttattc
     1621 cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt ttctcattaa
     1681 gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga agaaatcctt
     1741 taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa acgagattgt
     1801 atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc agatattcaa
     1861 tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca aaaaataaaa
     1921 ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt ttctattttt
     1981 acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt ggtcagtaga
     2041 aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct ttacgcaaat
     2101 gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac caccactttc
     2161 tctgattgac ggagcactac tcaaatactt agggattccc attgttctca ctgggttcat
     2221 gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta tcattaccta
     2281 tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa aagaaacaat
     2341 gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg attaaatata
     2401 tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat gaattaattc
     2461 cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat aatttctgtc
     2521 aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca accgaatcaa
     2581 gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta aaagagatag
     2641 cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt gacaatcaag
     2701 tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa
     2761 aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca
     2821 aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt tctaagatgg
     2881 aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg gagaaagatg
     2941 attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt gatgactacg
     3001 actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc
     3061 tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt gaacaggaag
     3121 ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa ttggatttcc
     3181 ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt gataccagta
     3241 tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat aaaaagaaag
     3301 aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg
     3361 tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc aaagaatcta
     3421 tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag ctgaaacaac
     3481 gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta cgaccttttg
     3541 gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat atcaatgact
     3601 atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct acacaaatga
     3661 ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga aatatttatc
     3721 tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca ttagcgtccg
     3781 cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg gtctattatt
     3841 cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga
     3901 aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg agtgagaacg
     3961 acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat tcagaaagcc
     4021 ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc gagaagtttc
     4081 ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg
     4141 tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct gaccatatcg
     4201 aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt gtcacccaat
     4261 ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc ttacccgatt
     4321 ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca atgctgattg
     4381 tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt aaaattgttg
     4441 accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta tctatgcgat
     4501 tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa aacgcggacg
     4561 atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt cgttccactg
     4621 acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa gatgaaaaca
     4681 atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac atctatggtc
     4741 gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc tttgatacca
     4801 gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat ttcttcacta
     4861 tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca cgctgggcaa
     4921 tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg aatactccaa
     4981 gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg gctggcttcc
     5041 atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg ccattactaa
     5101 ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg tgaaagaagc
     5161 ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat
     5221 tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt tcctccttct
     5281 ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc gagaaacgac
     5341 gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg cttcctttat
     5401 tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc
     5461 cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag ggaaagacag
     5521 tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat ggctattgct
     5581 tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc
     5641 taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg aagaaattga
     5701 agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag gaacagtctt
     5761 ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa caggaatcat
     5821 gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca gtttcctatt
     5881 aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt tatttaatgt
     5941 gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta tttccactat
     6001 gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac aaattgtgac
     6061 ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt tacaaagcaa
     6121 cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca
     6181 aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa
     6241 gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata ctcgtcaaaa
     6301 tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc aacaaggcgg
     6361 tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa atcgtctagt
     6421 caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg tcaaatatgc
     6481 ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac aatctatggc
     6541 agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa aacgaaagaa
     6601 cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa aagaaaaccc
     6661 atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc
     6721 tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa
     6781 acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa cggataagcg
     6841 aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt ctctctcttc
     6901 ttttttctct tattattcct agtcattttc tttgcagatg atacggacag tggcgaaaac
     6961 aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt gttggcacac
     7021 cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt ttcttatatc
     7081 ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca aagttccgaa
     7141 tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca aggggttaaa
     7201 tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga ttctgttatc
     7261 caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg gaaaaaatat
     7321 acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa agcagactat
     7381 cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg aaatcagttt
     7441 tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt tgatgatgaa
     7501 accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta tgtctttgga
     7561 ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt gtatgacaaa
     7621 gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca agaaatctct
     7681 atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa tgcaggcaca
     7741 tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc aggcgaccca
     7801 atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg ggctagacgt
     7861 gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc gaaaagaaaa
     7921 gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa
     7981 agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg gtagtgtcgg
     8041 atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg aaacagaaat
     8101 cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat cctttgcgaa
     8161 agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg aaaaattgac
     8221 ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta aggatattcc
     8281 tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga atgagaacac
     8341 ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca aggaaactat
     8401 atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg ttattatcaa
     8461 aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac agctagaaag
     8521 tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag aaacgttctt
     8581 ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta atcatgtgtt
     8641 acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct ttaccagaaa
     8701 agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa caaaggcaac
     8761 ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga ttgtaaaata
     8821 acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt gataaaaaat
     8881 tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta tttaaaagga
     8941 gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag cgggaacaaa
     9001 taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt
     9061 atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct atggttatgc
     9121 ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt
     9181 gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag
     9241 gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa
     9301 gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta
     9361 caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca
     9421 cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg
     9481 caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg
     9541 cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa
     9601 ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca
     9661 aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat
     9721 gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat
     9781 tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc
     9841 ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta
     9901 ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca
     9961 aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag ttttggagta
    10021 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
    10081 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
    10141 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
    10201 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
    10261 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
    10321 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
    10381 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
    10441 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
    10501 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
    10561 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
    10621 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
    10681 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
    10741 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
    10801 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
    10861 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
    10921 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
    10981 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
    11041 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
    11101 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
    11161 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
    11221 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
    11281 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
    11341 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
    11401 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
    11461 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
    11521 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
    11581 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
    11641 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
    11701 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
    11761 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
    11821 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
    11881 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
    11941 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
    12001 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
    12061 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
    12121 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
    12181 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
    12241 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
    12301 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
    12361 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
    12421 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
    12481 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
    12541 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
    12601 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
    12661 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
    12721 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
    12781 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
    12841 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
    12901 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
    12961 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
    13021 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
    13081 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
    13141 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
    13201 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
    13261 aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
    13321 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
    13381 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
    13441 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
    13501 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
    13561 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
    13621 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    13681 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    13741 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    13801 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    13861 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
    13921 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
    13981 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
    14041 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
    14101 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
    14161 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
    14221 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
    14281 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
    14341 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
    14401 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
    14461 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
    14521 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
    14581 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
    14641 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
    14701 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
    14761 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
    14821 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
    14881 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
    14941 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
    15001 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaacc
    15061 aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc tctatacatt
    15121 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
    15181 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
    15241 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
    15301 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
    15361 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
    15421 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
    15481 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
    15541 caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
    15601 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
    15661 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
    15721 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
    15781 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
    15841 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
    15901 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
    15961 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
    16021 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
    16081 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
    16141 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
    16201 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
    16261 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
    16321 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
    16381 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
    16441 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
    16501 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
    16561 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
    16621 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
    16681 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
    16741 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
    16801 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
    16861 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
    16921 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
    16981 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
    17041 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
    17101 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
    17161 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
    17221 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
    17281 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
    17341 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
    17401 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
    17461 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
    17521 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
    17581 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
    17641 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
    17701 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
    17761 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
    17821 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
    17881 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
    17941 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
    18001 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
    18061 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
    18121 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
    18181 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
    18241 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
    18301 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
    18361 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
    18421 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
    18481 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
    18541 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
    18601 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
    18661 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
    18721 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
    18781 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
    18841 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
    18901 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
    18961 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
    19021 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
    19081 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
    19141 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
    19201 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
    19261 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
    19321 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
    19381 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
    19441 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
    19501 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
    19561 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
    19621 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
    19681 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
    19741 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
    19801 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
    19861 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
    19921 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
    19981 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
    20041 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
    20101 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
    20161 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
    20221 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
    20281 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
    20341 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
    20401 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
    20461 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
    20521 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
    20581 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
    20641 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
    20701 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
    20761 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
    20821 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
    20881 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
    20941 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
    21001 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
    21061 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
    21121 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
    21181 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
    21241 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
    21301 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
    21361 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
    21421 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
    21481 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
    21541 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
    21601 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
    21661 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
    21721 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
    21781 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
    21841 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
    21901 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
    21961 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
    22021 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
    22081 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
    22141 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
    22201 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
    22261 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
    22321 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
    22381 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
    22441 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
    22501 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
    22561 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
    22621 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
    22681 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
    22741 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
    22801 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
    22861 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
    22921 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
    22981 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
    23041 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
    23101 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
    23161 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
    23221 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
    23281 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
    23341 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
    23401 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
    23461 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
    23521 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
    23581 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
    23641 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
    23701 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
    23761 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
    23821 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
    23881 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
    23941 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
    24001 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
    24061 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
    24121 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
    24181 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
    24241 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
    24301 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
    24361 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
    24421 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
    24481 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
    24541 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
    24601 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
    24661 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
    24721 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
    24781 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
    24841 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
    24901 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
    24961 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
    25021 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
    25081 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
    25141 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    25201 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    25261 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    25321 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    25381 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    25441 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    25501 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    25561 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    25621 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    25681 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    25741 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    25801 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    25861 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    25921 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    25981 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    26041 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    26101 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    26161 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    26221 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    26281 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    26341 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    26401 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    26461 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    26521 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    26581 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    26641 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    26701 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    26761 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    26821 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    26881 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    26941 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    27001 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    27061 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    27121 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    27181 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    27241 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    27301 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    27361 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    27421 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    27481 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    27541 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    27601 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    27661 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    27721 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    27781 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    27841 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    27901 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    27961 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    28021 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    28081 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    28141 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    28201 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    28261 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    28321 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    28381 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    28441 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    28501 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    28561 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    28621 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    28681 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    28741 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    28801 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    28861 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    28921 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    28981 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    29041 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    29101 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    29161 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    29221 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    29281 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    29341 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    29401 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    29461 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    29521 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    29581 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    29641 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    29701 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    29761 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    29821 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    29881 gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg
    29941 cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt
    30001 ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg
    30061 gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa
    30121 gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct
    30181 ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca
    30241 gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg
    30301 aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc
    30361 attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca
    30421 tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca
    30481 aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg
    30541 ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag
    30601 gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag
    30661 caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag
    30721 aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg
    30781 aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg
    30841 tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat
    30901 ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg
    30961 gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg
    31021 aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga
    31081 tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc
    31141 gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg
    31201 gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg
    31261 gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga
    31321 tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg
    31381 atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca
    31441 atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg
    31501 cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact
    31561 tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc
    31621 gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc
    31681 aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg
    31741 aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat
    31801 gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa
    31861 agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga
    31921 agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg
    31981 gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg
    32041 gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat
    32101 ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc
    32161 atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc
    32221 tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta
    32281 tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa
    32341 agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat
    32401 gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc
    32461 agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat
    32521 gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt
    32581 taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg
    32641 tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt
    32701 tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac
    32761 gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag
    32821 gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa
    32881 agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt
    32941 tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct
    33001 ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag
    33061 aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg
    33121 atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag
    33181 cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta
    33241 gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg
    33301 aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg
    33361 ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc
    33421 tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg
    33481 ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat
    33541 attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa aaagggactg
    33601 tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta ttttttagca
    33661 actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat ttcaagatcc
    33721 atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa tgagttacca
    33781 tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc atcttgccag
    33841 aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat atcgcgtttg
    33901 tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc ttccattaat
    33961 ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc gtatttttca
    34021 gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt taagcgccat
    34081 gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt acatgttttg
    34141 attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc ttctttgatt
    34201 ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac atcagcccaa
    34261 ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa aactaatttt
    34321 ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca gttctgtttt
    34381 attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
//
LOCUS       DANJJW010000019        33791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000019 DANJJW010000000
VERSION     DANJJW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..636)
                     /locus_tag="PWS82_000882"
     CDS             complement(<1..636)
                     /locus_tag="PWS82_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0906029.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKK"
     gene            complement(629..1294)
                     /gene="pgmB"
                     /locus_tag="PWS82_000883"
     CDS             complement(629..1294)
                     /gene="pgmB"
                     /locus_tag="PWS82_000883"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0906030.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(1287..3581)
                     /locus_tag="PWS82_000884"
     CDS             complement(1287..3581)
                     /locus_tag="PWS82_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0906031.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            3841..6006
                     /locus_tag="PWS82_000885"
     CDS             3841..6006
                     /locus_tag="PWS82_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0906032.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            6310..7143
                     /locus_tag="PWS82_000886"
     CDS             6310..7143
                     /locus_tag="PWS82_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL0906033.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(7245..8945)
                     /locus_tag="PWS82_000887"
     CDS             complement(7245..8945)
                     /locus_tag="PWS82_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906034.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            9452..10612
                     /locus_tag="PWS82_000888"
     CDS             9452..10612
                     /locus_tag="PWS82_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0906035.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            10628..11071
                     /locus_tag="PWS82_000889"
     CDS             10628..11071
                     /locus_tag="PWS82_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906036.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11554..12084)
                     /locus_tag="PWS82_000890"
     CDS             complement(11554..12084)
                     /locus_tag="PWS82_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL0906037.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            12189..12740
                     /locus_tag="PWS82_000891"
     CDS             12189..12740
                     /locus_tag="PWS82_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0906038.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(12893..13366)
                     /locus_tag="PWS82_000892"
     CDS             complement(12893..13366)
                     /locus_tag="PWS82_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0906039.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            13504..14976
                     /locus_tag="PWS82_000893"
     CDS             13504..14976
                     /locus_tag="PWS82_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0906040.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            14973..15683
                     /locus_tag="PWS82_000894"
     CDS             14973..15683
                     /locus_tag="PWS82_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL0906041.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            16035..16319
                     /locus_tag="PWS82_000895"
     CDS             16035..16319
                     /locus_tag="PWS82_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL0906042.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(16600..17019)
                     /locus_tag="PWS82_000896"
     CDS             complement(16600..17019)
                     /locus_tag="PWS82_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL0906043.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(17021..17605)
                     /locus_tag="PWS82_000897"
     CDS             complement(17021..17605)
                     /locus_tag="PWS82_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL0906044.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(17652..19127)
                     /locus_tag="PWS82_000898"
     CDS             complement(17652..19127)
                     /locus_tag="PWS82_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL0906045.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            19308..19619
                     /locus_tag="PWS82_000899"
     CDS             19308..19619
                     /locus_tag="PWS82_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906046.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(19734..22070)
                     /locus_tag="PWS82_000900"
     CDS             complement(19734..22070)
                     /locus_tag="PWS82_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0906047.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            22212..22880
                     /locus_tag="PWS82_000901"
     CDS             22212..22880
                     /locus_tag="PWS82_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906048.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            22883..23926
                     /locus_tag="PWS82_000902"
     CDS             22883..23926
                     /locus_tag="PWS82_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0906049.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(24163..25044)
                     /locus_tag="PWS82_000903"
     CDS             complement(24163..25044)
                     /locus_tag="PWS82_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0906050.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            25203..25931
                     /locus_tag="PWS82_000904"
     CDS             25203..25931
                     /locus_tag="PWS82_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL0906051.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            26087..26404
                     /locus_tag="PWS82_000905"
     CDS             26087..26404
                     /locus_tag="PWS82_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0906052.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            26397..27005
                     /locus_tag="PWS82_000906"
     CDS             26397..27005
                     /locus_tag="PWS82_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL0906053.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            27060..27851
                     /locus_tag="PWS82_000907"
     CDS             27060..27851
                     /locus_tag="PWS82_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL0906054.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            27989..28501
                     /locus_tag="PWS82_000908"
     CDS             27989..28501
                     /locus_tag="PWS82_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL0906055.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            28527..29399
                     /locus_tag="PWS82_000909"
     CDS             28527..29399
                     /locus_tag="PWS82_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0906056.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            29575..29937
                     /locus_tag="PWS82_000910"
     CDS             29575..29937
                     /locus_tag="PWS82_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0906057.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            29946..30698
                     /locus_tag="PWS82_000911"
     CDS             29946..30698
                     /locus_tag="PWS82_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906058.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            30712..32514
                     /locus_tag="PWS82_000912"
     CDS             30712..32514
                     /locus_tag="PWS82_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906059.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            32791..>33791
                     /locus_tag="PWS82_000913"
     CDS             32791..>33791
                     /locus_tag="PWS82_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0906060.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     "
BASE COUNT        10591 a         5964 c         6951 g        10285 t
ORIGIN      
        1 tttttttgtc caatttccag taggcagttt ttcttgatct aattcaagca caccatctaa
       61 aaagaccgtt aactgatgtt catagatgtc tcttgttcca tttcctgaaa ggttgaagta
      121 ggcatggttg gtcggattgc agatggtttc aacaaggcta ctagcagagg ttgtcatctc
      181 taattcgttt tttgttaaac ggtaagtgat cgtagcagtg attggtcctg gataggtaga
      241 aaattcatct tttaagtaaa agaccacacc tattgatgat ggagttttga aaacttctac
      301 ttcccaaaat tggaaagacc agcctttcgt gccaccgtgg atatggtgtg atccgcagtt
      361 tttctccaat ctatgctcac cccaagaagc atcccggatc cttccggcta caggtccgac
      421 agttgctcca aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt aagacaataa
      481 aacattttct gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc gggcaccgta
      541 attgagaaga acgacttcta agtagtcgtt cttcaatgtg attttcttta tttcagtctc
      601 tggaactggt acgacggaga tctctggatt atccatggtc atgacacttt tctttcaagt
      661 aatcgatcgt aagaaaagtc gtatcaggga caagcgggat ctcctctcca aggtcttcag
      721 cacgtcccac gccaattggt tgagcgccac ttgagaggat ggcttggata ccggcttgcg
      781 cgtcttcgat cccgatacat tcttctgctt ttagtccgac agcttttgct gcaaggagga
      841 agatatctgg cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata gcatcaaagt
      901 aaggtgtaag acccattttt tcaagtagga atggcccgtt tttacttgca gaagcaagag
      961 caatcttgat tttttcttct tttaaagaat ccagtaaagg cacgaccccg gggaagacat
     1021 cttttggttc gatcgtctgg atcatctcta aataataatc gttttttctt ttcgctaatt
     1081 ctgcaaattc ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa agcaaagaga
     1141 gtgagtcttc tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg atcgtaatgc
     1201 cgatttcctg accaagcttt cgccaagcac gataatggaa ttcagcagta tcggtaatga
     1261 caccgtctaa atcaaacaat acccctttaa acatttgctt cctcctttct tagcgttatt
     1321 tctagacgat ccgctaaaga ataagactgt tcatagacgt aaatttctag aggaaaacct
     1381 tctaataact cgaaagtaac ttgttcatct gtgactgaga tgaacaataa acgtccgcga
     1441 taattgatat ggaaagaata agctttccaa gtttttggca gaaatggtgc gaatctcaac
     1501 tgatcatggg cagttttcat ttgagcaaat ccttggacaa tcgacaacca gctaccagtc
     1561 atggaagtga tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa atccaagcga
     1621 gctgttcgct ggtacatttc tacagctttg ctttccattc ctaattctgc tgctaaaatc
     1681 gaatggatac ttggcgaaag ggaagactca tggacagtca ttggttcata gaaatcaaag
     1741 tttttttgct tttcttcttt tgtaaaagta tcattgaaga aataaatccc ctgtagtaca
     1801 tctgcctgct tgatgaagca agatctaagg attttgtccc aagaccaatg ctgattcaga
     1861 ggaagatcgc ttgtgggaat atcggataca ggcatgagat ctttatctaa gaatgtgtca
     1921 tgctggacaa agattcctaa ttcttcgtca actgggaagt acatgttctc tgcgatatct
     1981 ttccatttgg ctagttcttc ttcgctgata gtgatagcag cttctttttg ataagttaaa
     2041 taattttctc gcgtgtaacg caagacccaa gctgcgatcg tatttgtata ccaattgtta
     2101 ttgatattgt tctcatattc gtttgggccg gtcacaccgt ggatcatgta tttatttgct
     2161 cgttttgaaa aatggacacg gtctgcccag aagcgtgcga tttctgctaa aacttccagt
     2221 ccttcatttt tcaaatattc cgtatcgccg gtgtaattcg tataattgta gattgcatag
     2281 gcaatcgcgc catttcggtg aatctcctcg aatgtgattt cccactcgtt atggcattcg
     2341 acaccagtga aggtgaccat aggataaagc gcgcctttta atccttgttg ctgagcgttg
     2401 tggattgctt gcggcagttg attatgccga tatttcagca gattcttagt tacttcaggt
     2461 ttggctaaag caagataaag agggacagca tacgcttctg tatcccagta agtggctccg
     2521 ccgtattttt ctcctgtgaa tcctttgggg ccgatattca atcgttcatc ttcgccgtag
     2581 taagtagaga atagttggaa caaattgaat cgaatacctt gttgtgcttc ttcgtctcct
     2641 tcgatgacca catcggcaag cttccatcgt tctgcccaag ccgctgtttg ctcttctttt
     2701 gcttggatat aattgtttgt ctggttcgtt aattctgaga cagctttttc tgcttgctgc
     2761 gcttctggaa tatctctact agtaagaacg ataatttctt tttccactga ccatgtctca
     2821 ccttgagata attctgcaga caagcaacca tgtaatgcta aagtatcttc gtgatagtca
     2881 gggatgactg attttccatt aacgaaatgg cgcattgctg cagtaacagt aaattgttca
     2941 atcccaaagt tgtttggaat agttttagtc gtcaaatagc cgatttcttc tatagttcca
     3001 cgatcaatct cattccaaaa atgttcatca tagttgctgt cttcgttttg aacatttcca
     3061 tccaattttg atacgagttt gactgttccg ctaccttcaa gcatttctat agttaaatag
     3121 atataagctg attctttttt gacaatgctc agaaaacgtt cgaaggaaat gcgtacttta
     3181 ttagtagaag tagtgattgt gaatgtacga gataatacac catttttcat atctaattcc
     3241 cagtaaaagt cttctggttc caaagaagcc aagtcgatca gctgatcgtt gatcgaaata
     3301 tctatcgcga taaagttcaa ggcgttgatc acttttccaa agtattctgg ataaccattt
     3361 ttccaccagc cgacacgtgt cttatcggga taccagacac ccgccaaata agtaccttga
     3421 tggtggtccc ctgagtaagt ttcttcaaag ttcccgcgca tccccatata accgtttcca
     3481 atacttgtta aagattcttg cagtcgaaga ttttttttat ctaattgtgt cgtgctgatt
     3541 ttccatggat caatagcaaa tagtcgtttg atctgtttca tttttctcct cctaatgagc
     3601 tgttttatac gttcataata aacgcaaccg attgcacaag tcaatagaca aaagaaaata
     3661 tttcatagaa aaaagcttaa aaaaataaat ttttaggaga aaattataga agggaacaca
     3721 cgtattatag gttttataga atggtataga cagaaaaata aaaaataaag catttacaaa
     3781 aaagaaaacg cacgctataa tacgtttaac gaaaccgatt gcgtaaagga gctaaacgga
     3841 atgaaaaaga tatttagttt tgagttttgg caaaaatttg gaaaggcttt aatggttgtg
     3901 gtagcagtta tgccggcagc tgggttgatg atcagtatag ggaaatcaat tcccttgatc
     3961 aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat tggttgggca
     4021 attattggta atcttcatat tttatttgct ttagcaatag gaggaagttg ggcaaaagaa
     4081 agagcaggcg gagcatttgc agctgggatc tcatttattt taatcaatcg gatcactggg
     4141 gctatctttg gtgtcacaag tgacatgctt gcgaatgaag atgcatttac ccacactctg
     4201 ttcggtacga agatcatggt taaaggattt tttacaagtg tattggaagc accagctttg
     4261 aatatgggag tattcgtagg gatcatcgct ggatttgtcg gggcaatggc ttataataag
     4321 tactataatt atagaaaatt gccggatgct ttgtcatttt tcaatggaaa gcgctttgtt
     4381 ccgtttgttg ttatcttatg gtcaacaatc gtggcattga ttcttgctgt ggtgtggcca
     4441 aacgtacagg ctggaatcaa taattttggt ttatggattg cagaatcaca agaaagcgca
     4501 ccgattctag caccgttttt atttggaaca ttagaacgtt tactattgcc atttggattg
     4561 catcatatgc tgacgattcc tatcaactat acacagcttg gcggatcgta tgagatttta
     4621 tcaggcgcac aggcggggac acaagtattt gggcaagatc cgttatggtt ggcttgggcc
     4681 actgatttag tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt tgtgttgcat
     4741 aactggacgc cggctcgttt caaagtagga caaatgattg gttcttcagg tatcttgatg
     4801 ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag atataaatca
     4861 atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc attggagttc
     4921 atgtttatgt ttgctgctgt gccattatat gtcatttatt cagtgatcca aggggctgca
     4981 tttgcgatgg cagatatctt gccattgcgg gtccattcat ttgggaatat cgaattgctg
     5041 actcggacac cgttagctat caaagcgggt ctaggtggag atcttatcaa cttcgtgttg
     5101 tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa atttcttgat caaaaaattc
     5161 aactttgcta caccgggacg taacggaaat tacgacaatg acagtgaaga aaccgttgta
     5221 tctaacagcg gaacaggaac agcagatcag caagtggtcc agatcattca tttgctggga
     5281 ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt aagtgtaaat
     5341 gatcgagaaa aagtagggac tgaagaagcc tggaaacgag caggggcgat gggattgatc
     5401 gtcaaagaca atggagtcca agcagtttat ggaccaaaag cagatgtctt gaaatcagat
     5461 atcgaagatc tgctgcaatc aggagcagag attccagagc cagaaatggt ttctacagga
     5521 aatggacaag caggtagtaa gcaatactta ggtatagaac aagaactagt ttccgcagct
     5581 tctggagaag tgattccttt atctgaagta aaagatccgg tattctcaca aaaaatgatg
     5641 ggtgacggct ttgctgttat tccaacatca agagaagttg ttgcgccgat cgctggaaaa
     5701 gtgacaagta ttttccctag caaacatgca attgggctgg aaacgaaaga tggtatcgaa
     5761 gttttgatcc atatgggaat cgatacagtc caaatgaatc agccagcttt tgagattgct
     5821 gtcaaagaag gccaagaagt aggaacagga acgaaattag ctgagatgga tcttgatgtg
     5881 atccaaaacg aagaaaaaga tccaacgatc atgatcgtat ttacaaacga taaagtagaa
     5941 gaagtagtga tcaagcaatt aggtacagtg acagctggta aagtgatcgg ctccattaaa
     6001 atgtaaggag aggttgtgaa aaagctgtcc tgcatcaagt cataagaaca gccaaacaaa
     6061 aatagctcct cctatttttc gtttggttgg gcttattacc gagaagcagc agtttgaata
     6121 ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa acagccaaac
     6181 aaaaaatagc tcctggtcca ttgagcttat tactgaggag ttagcaggag aacactattt
     6241 gctggagagg ttgtgacaaa aattttgtca caaccctttt gtttcgaaat gaagacaagg
     6301 agaaaaccaa tgaaattcat gacactcaac acgcatagct ggcttgagcc agaaccagag
     6361 aaaaaattgc aagaattagc agataagatc ctgttagaag attatgaagt gattgcttta
     6421 caagaaatca accaattact ggaatctgaa gaagtagaac ctgcgaagct aatgaagttc
     6481 tgtcaggtaa agaatcaagt acctatccgt aaagataatt ttgcctaccg tttagttcag
     6541 cttttaaagg aacacggaaa agagtatttt tggagttggg aaatgagcca catcggttat
     6601 gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag tcaggtcctt
     6661 accgtctccc aatctcaaaa gaaagaagat tatcagacac gcaagatctt gattgggaaa
     6721 acaaagatag atgatcagga catttttgtc tcgagctgcc acttttcttg gtggacagat
     6781 aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa actacttttt ggaaactcgc
     6841 gtaccacttt ttttcctagg tgatttcaat aatccagtag actctcaagg ttattatact
     6901 gtaagagaaa gttgccttct tcttcaagat tcgtatgttg tcgcgaatga aaaaggaaaa
     6961 gcagctactg tggaaaagaa aatagatggt tgggaacaaa atacagaaaa attgcgtata
     7021 gattttattt tcgtccctga aggaatgcag gttaaaaaat atcaacggat ttttgatggg
     7081 atcgatagtc caatcatcag tgatcactac ggggtggaaa tcgaattaga tgtgaacgaa
     7141 taaaaaatat aacatataac ataaaaaaac tgattaaaat aaaactctcc aaacgattat
     7201 accgtttgga gagttttttg atcggatgta tttatccctt ttgcctaagc acgtaaaagc
     7261 ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa agtaagagaa
     7321 agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt gattcctttt
     7381 ttctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa tgtttttcca
     7441 ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt tttgtcccca
     7501 gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa agttgtttcg
     7561 ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc cttatagatt
     7621 ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat atctttgctg
     7681 aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg actgacaaat
     7741 gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg tgctgaaaaa
     7801 ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca ctggttcagt
     7861 gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg aaatggaaac
     7921 tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg aatcaagcta
     7981 gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa taaggtgttc
     8041 ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg aaaggcctta
     8101 gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat ttctctgtta
     8161 acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc ccaaatattt
     8221 gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc ttttcctttt
     8281 tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc taacatgatc
     8341 gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt gataacaggg
     8401 aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata aaaacaagtc
     8461 ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt actcatgaat
     8521 aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag taaaatcagc
     8581 atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata atgaaaccag
     8641 ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc gtaaacggca
     8701 ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg gaaatcccca
     8761 tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc atcagcaccc
     8821 aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat gaaaaaaatt
     8881 gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt agataaattc
     8941 atcatatatc ctatgcgaga ggtaggcaag aaattagaaa attcatgaaa atatttatga
     9001 attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca taagttttgt
     9061 tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt agctttttca
     9121 tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc actagcctag
     9181 aaaatatgag aagctgtttt ctgactagcg ctcttattac tcaaagcata acgcagtttt
     9241 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccca
     9301 acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc aggacagctt
     9361 tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat aataaaagaa
     9421 agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa aacagtctcg
     9481 tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac tgatcgcttt
     9541 atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta tcgaacaagt
     9601 acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg agaaaaaaca
     9661 acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa agaacagaaa
     9721 tgaaagtaca acatccagcc aattagagga atggctgatc ggaagtatca atatacctaa
     9781 gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg aaaatggagc
     9841 aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt cagtgatctc
     9901 tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt tagaaaacgg
     9961 agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta aaaatataca
    10021 agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag acttagttac
    10081 cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga caggacatcg
    10141 gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg aaagaaccat
    10201 gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt tagtgtttat
    10261 cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt catttattat
    10321 tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac ataagaaaaa
    10381 aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg ggaaagtcaa
    10441 gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc ctaaagttgc
    10501 tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg aggggcgaaa
    10561 acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat aagtaaggag
    10621 agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat ctgttctatc
    10681 gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt attgggagaa
    10741 tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag cagaaataga
    10801 tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa tctttgtgaa
    10861 aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa aagaaaccaa
    10921 taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt tctatctttc
    10981 acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg aggaacttgt
    11041 gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata atgctcccag
    11101 cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac tgtgtgaata
    11161 tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg tattagctca
    11221 aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact caggtcaaaa
    11281 cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg gtaataagcc
    11341 cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta ctcaaagcat
    11401 aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct cggtattagc
    11461 tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat actcaggtca
    11521 gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat aattttcatc
    11581 gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt cgctaataac
    11641 atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt caggaacaag
    11701 taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga tatgttcgtt
    11761 ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa agatcattcc
    11821 gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg ttggctctgt
    11881 ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt ggtcttttac
    11941 ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt catttttggc
    12001 ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc caataaaatc
    12061 ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa acatgtaata
    12121 aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt gtcaaggagg
    12181 gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta ttaagtaaga
    12241 agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga gaattttggc
    12301 aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa ggaatcattc
    12361 agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa ttaacagaag
    12421 aagggaagga acaattggca gagtggattc atacaccaac caatgaacta ccggttaatc
    12481 gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg gcattagtag
    12541 aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta ttagagcgaa
    12601 aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac tacctgattt
    12661 tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat gtattagaag
    12721 aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc tttctatatg
    12781 aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt tttcaagtat
    12841 ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc gtttagagtt
    12901 cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg taagttaatt
    12961 cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg aagttaccga
    13021 tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct tttgaaaaaa
    13081 ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca gcttttgaca
    13141 aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact gtaggtaaac
    13201 gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc ttttgttccc
    13261 aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg atgccaacaa
    13321 ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt gtcactcctt
    13381 ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt aggttgacaa
    13441 atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga aagaaggaga
    13501 aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg gacactttta
    13561 gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt attgacgaca
    13621 gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca atggttaacg
    13681 actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct gatcaataaa
    13741 ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg aacgattact
    13801 tgctttacag cacctaattt tagtacatta ctaatcggtc gcttgatcca agcttgcggc
    13861 gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc gccagaaaaa
    13921 agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc tttaggccct
    13981 acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg tatggtactg
    14041 ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt tattccattg
    14101 tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt cggtagttta
    14161 ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt aattggtttc
    14221 ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca tttggaacat
    14281 ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc tgttttagct
    14341 ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta tattcaaaat
    14401 attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc attgatcatg
    14461 ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa acgtttagcc
    14521 atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt gacaaaaacg
    14581 actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat ctccatggtc
    14641 atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat cagtcatggg
    14701 acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc tattttgatc
    14761 agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt ggataatacg
    14821 ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca tgccgcgttt
    14881 atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt gaagaaaaaa
    14941 gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg attgtcagct
    15001 tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc tttttatctt
    15061 tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat ttgtccaatc
    15121 actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc agtgctgatt
    15181 cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa aaagtgtatc
    15241 tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat aacgaaacaa
    15301 ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact tattgggaat
    15361 acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac gaatatgaca
    15421 gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg gaccaagcag
    15481 ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa gcaaaagctt
    15541 atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc aaagcagagc
    15601 aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc gcaaaattag
    15661 tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggaggttgt gaaaaagctg
    15721 ctctgcatca agcagtaaga acaatcagat aaaaatagca ctccatattt ttcatctgat
    15781 tgggcttagt gccgtagatg caagcttttg aacaccgttt aaggaagagg ttgtgagcct
    15841 gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgac
    15901 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgaa aaaaggaact
    15961 gcgacaagac ttttgtcaca gttccttctt ttttttgcta tgctatacta gatgtaaaaa
    16021 ataggagaaa taaaatgagg gaaatttatt cttttgaatc aaaaatctat gcttccgaag
    16081 tcgacaaggg cggggcatat attatttttc cgtatgatat aagaaaagaa ttcggtgctg
    16141 gaagagttaa agtccaagca acttttgaca atatgccgta tgaaggcagt atcgtgaata
    16201 tgggcgtgaa aaatccagat ggttcagtct gctacatcct tggactacga aaagatattc
    16261 gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt tacagtgaag caaaaataaa
    16321 agaggttgtg acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa aagccagttt
    16381 ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa aatttttctt
    16441 actactcgaa acgaagcggc tttatcacaa cctcttgata aacggcgttc aatcagctgt
    16501 ccctcggtat tagctcaaaa acggcaaaat atgagaagct atttttccat ttttttctct
    16561 catactcagg tcataacgct gatttcacaa cctcttcttt cataatgttt tttcaccatc
    16621 tttataataa actggttctt tgatatggaa atctattttt tccaaccaat ctaaaatctt
    16681 gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg agatatggtc
    16741 aacatggata ccggaagtga tcgtacaact tcccggaacg gcagttttga tgatcacagc
    16801 tattttttcg gctaagacat cgtctttatg gaaccgacca tcgtggctag ggaaacgtac
    16861 agtttgcatc tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg gtgtatctcc
    16921 gcctgttaaa gtgatcaata agtctttacc gattacttta acagcagctt ggatcgtgaa
    16981 acctagtttg gttacttcaa ctatcgtttc tttttccata ttaatcaccc tgccaacgtt
    17041 ttgaatgctc atttacaatt ccaaattgat ccaaaagttt cattgtatgg tgattaacaa
    17101 gatcttggat cgtttgaggc tgattataaa aagcaggaat tggtggtatg atttgcacac
    17161 ctagtcgtgc taatttagtc aagttttcca agtgaatggc atttaatggc gtttcacgag
    17221 gaacaatgac taactttcgt tgctccttca ttgttacatc agcagcacgt gcaatcaggt
    17281 tgtcgcctaa accaacagag atacctgcga ctgttttcat acttgccggt acgatgatca
    17341 ttccatcggt caaaaatgat ccgctggcga cagcagcacc taaatctttg tttgaataat
    17401 ggtaatcaaa caggctttcg aattcagtta gtgataagtc ggattccagc tttaagttct
    17461 gttttgccca tggactgatg attccgtgtg tttctatcat cggatattct tttagtttct
    17521 tcactaggtt gatggcataa atggtgcctg aagcgccact tacaccaatc acgatttttt
    17581 tcttccgttg tgccaattgt tccattttcc gcactccttt ttatgatgtc aaaacagttg
    17641 cagatcattc attatttcaa atatttttcc caatcttcta cttcttggaa ttttgcacgt
    17701 acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa agatagtttt tgcactcatc
    17761 ccacggaaac gaatagagcc aggattatat tctggtaatt ctgatgggtc caatggatgg
    17821 ttcctcattc ctgggatcac gatgatatct ttatctcctt ggaaacgagt gttgaaagtc
    17881 caaactacat cgttcatgtc gaaaatatca acatcttcat cgactaaaat gactgtcttc
    17941 aattctttga aggctgataa agcaagaata gctgcttgac gctgaatacc ttcatccgct
    18001 tcgttttctt tacggatttg catgatggtc atcaatttac caccacctgc aggaggattg
    18061 tagacattta ctacttttcc tggaatagct ttatcgacca aggcaagaat acttgcttct
    18121 gtaggaatac cggccataga tacgtgttct tcactgggac caatcgttgt ttgcatgatt
    18181 ggattgtctt ttcggtgagt aactgctttg actttcacta cttgtacagc tggattagca
    18241 tccccagtat agccaggaaa ttctggcatt gcttttcctg agttcgtgtt gatatcttct
    18301 tgcattgttt catttggcat gatttctgct tcaatgatga attcagcacg agcaataccc
    18361 acttcatcga ctgctatacc atcaacgatt tgaataggtt cgttacgcaa agctcctgca
    18421 caccatagtt cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt gccaatggca
    18481 attgctggat ctaaaccaat attgatagtg ataggcattg gtttattcaa ggcttcgaat
    18541 tgtttttgga agtgaccaat gtggcgtccg ccaggcatga tgtacatacc gatcgtatct
    18601 ttatcttcta gaaccatacg atggatcgtt acatcgctca ttgttttttc tggatctgat
    18661 cctaagacta cacccattgt gatgtatgga ccagcatctt caggagtatt tgtaggtgca
    18721 ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta gatgaacgac ttcttgtgct
    18781 ggagcgtctg ctttgtctac tttcacaggt ttgattggat tttctacagc gtctttcaag
    18841 aaacgaccaa gtgttcgata atcatggtgc aaaatttcac ctacacgttt acgactagcc
    18901 attgttccga tgttcacacg tgtgccgggg aaacctttga tattgttgaa agtcatagct
    18961 ggtccttctt gagtcggacg ttttactgta ccgccagcac cgatataacg gtaaacacca
    19021 gataattctg cttcaggatc gatttcaata tctgtttcgt gatattgacc tggaagtgtt
    19081 tttagttctt cgatgacttt tcttaaatca tacggttgat ctgtcatgag agattcctcc
    19141 ttagttgtct ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat tcaacttgtt
    19201 cggtaaagta ttctacaatg gaatggatgc gtttttttga taatagaata aactatgata
    19261 tacttatcac taaaagaaag cgcatactta gtgttggagg aaggaaagtg aaaaaaatga
    19321 aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc gaatattttg aaagttacat
    19381 ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac agaagagata accgatgcct
    19441 tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt tctcgtaata
    19501 tgtggattgg gactgaagta acgattgaag cagatggaac agtattcata aatggaaaag
    19561 acaggtatac gccagaagag ttgtggtaca aaggaaagaa aagtatacca gaattataaa
    19621 aaataattaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt tcttgctgtt
    19681 ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca accttattct
    19741 gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag atgaccgcca
    19801 agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg atttagttga
    19861 gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa cgtagctagc
    19921 catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt ttctgcgtca
    19981 aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc gatttctttt
    20041 gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact tgttactaaa
    20101 gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc gccagtcagt
    20161 tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga tttgccttta
    20221 ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt cggataaatc
    20281 atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg tttggcattt
    20341 tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc tttgacgatt
    20401 tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata agcgattcct
    20461 ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga gatcttcaat
    20521 tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa attcccagtt
    20581 ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc aacgatatca
    20641 tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa tgcgttgata
    20701 ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc tggataactt
    20761 ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc aagctgtgta
    20821 gggaaacttg acactcccac acctgtcatt gctgacatcg tactagataa agaaagagat
    20881 tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt tacatcacgt
    20941 aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt aggatcgatg
    21001 tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc tttatcttct
    21061 tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct tgcacccaat
    21121 ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg aaatttcgcc
    21181 atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag ggacagaacg
    21241 atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt acgtcctttt
    21301 tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat ctttgttctg
    21361 cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc tgttaagatc
    21421 ttaccatcag aaaattcaat gatccgatct gcgtattgat gggccagttc aggattatgt
    21481 gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat gatctggata
    21541 cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc tgggtcattg
    21601 gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg atttggcttt
    21661 ttatgcatat gatctgtcaa gccgactcgg tgcaatgcat cttctgcccg ttttcgtttt
    21721 tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat aatgcctaga
    21781 tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg gtaggcatcc
    21841 cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc accggaatca
    21901 taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc actaggacct
    21961 aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa tgctttggtg
    22021 gtggtttcgc cgactttata atattttttt atatctttta attgaagcat aatcaccctt
    22081 ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat cgtagtatac
    22141 gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt tcaaaaaaca
    22201 gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta cagttgcttt
    22261 accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat ggagaggatg
    22321 cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc atgatgccga
    22381 aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac acacctgttc
    22441 taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa ttaggagcag
    22501 atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg gaagcattgc
    22561 ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca aaattagagc
    22621 tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa aaagaatttc
    22681 tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa cagttgatgg
    22741 atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta catatcaaac
    22801 gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta cggggactag
    22861 gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat ggatctattt
    22921 tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg gttttatttt
    22981 cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa cgttataccc
    23041 gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga tttttgttgg
    23101 gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg tagcaacggg
    23161 agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc aactgtataa
    23221 agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga atgactttgt
    23281 gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat atgttcaatt
    23341 gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc ggattatcga
    23401 aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca aactggaaaa
    23461 ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca gagaagtaat
    23521 cgtttttttg cagcctaaat gggagaaaga acagctagaa ttggatatcg aacttgctgc
    23581 agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata tcatggataa
    23641 cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg agacagccac
    23701 agagattgga ctggaagtga ccgattcagg tgtaggaatg aatgaagaga ccagagatcg
    23761 aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga atgggctagg
    23821 actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt atagcagtat
    23881 cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa aagaataaaa
    23941 cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg tgattaggga
    24001 aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac tacgagaaat
    24061 gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag tcatgcgtcg
    24121 ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt cttcttgatt
    24181 taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac gagtaaatgc
    24241 aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt ttaatagatt
    24301 cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca tattgatgat
    24361 caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt cgcgacacag
    24421 atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt tctccttttt
    24481 cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag gtagaactgc
    24541 tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg cctggaaatt
    24601 tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta attcgacact
    24661 ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact cggtgatttt
    24721 ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg aaagattgtt
    24781 gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta cgacattttt
    24841 tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat gggcgcgttc
    24901 gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa tatttccttg
    24961 ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta aatcaagaaa
    25021 aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa agaatacttt
    25081 aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct tgcagcgaaa
    25141 gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag aggagttttt
    25201 tgatgccaac tggagttttg atcaatgtag gttccgtttt gcttggcgga ctaattggcg
    25261 gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg gtttttggtg
    25321 tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg ccggctgtta
    25381 tttttgcttt agtgatagga acagcgattg gtctgattgt tcatttaggt aatgggataa
    25441 ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt gaaaagcttg
    25501 gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta ttttgtgcaa
    25561 gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt acgattttga
    25621 tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat ttagggtatg
    25681 tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc ttattggcaa
    25741 aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct tgcggcggat
    25801 tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca gtagctgaca
    25861 tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat tggattatgc
    25921 cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag gatcaaatct
    25981 tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata tgttgtataa
    26041 tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc acatccagat
    26101 tccaactgtt cttcttgatg gtactgtgct agcagcatta agcaaggaag atatgtatgg
    26161 gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta cactatatcc
    26221 ggttttgaga agattgaaaa aagaaggata tcttgagacc tatgatgaac cttttgaagg
    26281 cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag aagaaattat
    26341 tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga gcacaaatga
    26401 atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac gatcgttttg
    26461 atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta gatcagatca
    26521 ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt tacttttcag
    26581 aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt cgcctgattt
    26641 ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca ctggggttta
    26701 tattggttat tgggggacta ttggttgctt tactagcgct catttttagt atctatgttt
    26761 tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc agtgtgtttg
    26821 gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc acaggaagcg
    26881 cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat gtcatgatga
    26941 actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat tcagcgggcg
    27001 tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt attggtctga
    27061 tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa aagagtctga
    27121 cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa atcaaggatg
    27181 caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc ggattggatt
    27241 ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag aaaaatggcg
    27301 tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta gaagacacaa
    27361 gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg acaatcgaag
    27421 ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat actaagacac
    27481 atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg aaagatgaat
    27541 taggctcact tcgattagat gaggccaatg tggataactt gatcgtcaca ggtgaaaaca
    27601 gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc ctaaacggtg
    27661 gcaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg tctggtgaat
    27721 cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa ggacagaaaa
    27781 agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa gaggacaatg
    27841 aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa gatccttcca
    27901 atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga aagaaaatat
    27961 ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa gagaaaagtt
    28021 aaaaacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa ccttctatga
    28081 tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc gccaattaga
    28141 gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc tggattatta
    28201 ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg aacgatttat
    28261 ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag aggtcttggt
    28321 agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt atttaccgca
    28381 ggcagccgga gggacacagg caaggggttg gtacatcact gaagacaaag agttagaact
    28441 agaaggtgta atggcgcgta ttcgaaaaaa aattaaaaaa gtaacattag atatttattg
    28501 aaggataaag gagcgcagga agtagcatga gagaaaaaag agcattttat atcaatggtt
    28561 atgcagggat tgtcggattg gtcgtattag ctttagttgg attgtttttc ttctatttag
    28621 gtatgtggca agcgaaagtt tgggcacttt ttttaagtat ttttctttgg ttgatcacgt
    28681 tattgttgtt aagttcggca actgtagtca gtcctaatca agcaaaagta attttgtttt
    28741 ttggacaata tttaggtacg attcgagaaa atggcttttt ccttacgata cctttggcac
    28801 aaaaaatgac tgtttcgttg aaagttcgga atttcaatag ttctgtttta aaagtcaatg
    28861 atttggatgg aaaccccatc gaaatctcag cagtagtagt ctttaaagtc gttgatacag
    28921 caaaggcatt attcgatgta gcgtattatc aagattttgt agaaatccaa agtgagacag
    28981 ccattcggca tatcgctagc caatacccgt atgatacatt caatgagaat gatttgacgc
    29041 ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa agagctgcaa gagcgtttag
    29101 cagtagcagg tgtcgaagta atcgaaacac gcttgaacca tttagcctat gcaacagaaa
    29161 tcgctagtgc catgctccaa cgtcaacaag caaaagccat tttgtctgct cgacaaacca
    29221 ttgttgaagg agctgtcaca atcactcaaa tggccttaga gcaaatcgaa gatggacaag
    29281 aaatcaattt caccgatgat cggaaagtcc aattgatcaa taatttactt gtctcaatca
    29341 ttacagacaa aggaacgcaa cctgttatca atacaggcga catgaaagac cgtaagtgac
    29401 atactttttg taagatagga agaaggagtt taggacggat tattgtcttg aactcttttt
    29461 ttagttgttt taaagaaaat gacaaaattg aaagaagaat gatagttgat tcgaaggcag
    29521 aattgttcgc ttttttctat aatcagtata taaagtaaga gagaagggga aaaagtgaaa
    29581 aaacttctga tcggtctact tgttttcatc gttgtggtac ttgcaggggt gaaaatcgca
    29641 gattacgtag ttatgggtgg agaagaatac tatgtacaaa ttacgacaga tggtacaaaa
    29701 acagtttcaa aatcagactc tggacaagaa acagtcaact atagatatat cctgcctggc
    29761 tatacgaaag agggtaagga aaaagaactg gacttcaagg gacaattgac tcgaccgctt
    29821 cgtaaaggag catatttaaa aatcacctgg aacaaaaata aaggtgtcac ttcgtatgaa
    29881 gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa aactaattaa agggtaggga
    29941 aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa agtatacgga aaaaacaatg
    30001 agaaaaagac gcaagcatta agcggcatca gcttcgatgt agaaaaaggc gaatttattg
    30061 ggatcatggg cgcttctggt tctggtaagt cgacactttt gaatatttta tccacattag
    30121 ataaaccaac agatgggcat atccgaatca atcaaaaaga tgtgaccaca ttaaaaggaa
    30181 atcaactagc agattttcgt gcgaatgaga ttggctttat tttccaagat ttcaatttat
    30241 tagaaagctt gacagcacaa gaaaatatcg ctgttcctct ttctttacaa ggcgtcagac
    30301 caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc tctcatattt
    30361 tggaaagcta tccttctgag atttccggtg gacaaaaaca acgggtcgcc gccgcacgtg
    30421 cattgatcac acaaccaacg attctattag gggatgagcc tacaggtgcg ctggattcca
    30481 aaagtgcccg ggatctttta gatacgatgg acgaattgaa tacaaaagat catgtatcga
    30541 ttcttcttgt gacgcatgat ccgttttctg caagttattg ccaacgtatt ttattcatca
    30601 aggatggcgg tatccatcaa gaagtgaaac gtggagatca aagccgtgat tccttctatc
    30661 gagaaatact atctattctt ggaaacttgg aacagtaggg ggagattgac tatgctttgg
    30721 aaattatcac ttactgggat caagagccgt ttaagagact acttggtgtt attctcagga
    30781 ttagttatgg catctgctat tttttacatg tttgaatcaa tggcaagcaa cgaagctttt
    30841 ttgaaaagta atacgatcgt tgcggctaca gttatcattt tccgattcgg ttctgtttta
    30901 ctagggatca tcacattcgt ttatatttta tatgcaaatt cctttttgat gacgatgcgg
    30961 caaaaagact atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat cgcacagatg
    31021 attttttctg aaacattctt agtagggatc gcagctactt tagtgggttc agctgtcggt
    31081 gtaggattga caagtatcgt ccaccgatta ttagtggatc agttagatct caatatttcg
    31141 cattttacac cttttagtat tcacgggttg ttgatcactg ttttgttttt tgctgttttg
    31201 tttttattag ctgctgttgt caatgctttt tcgattgtca aaaaaccgat tttagcattg
    31261 attcgtgcgg atcagacacc aacaagaatc aagcagcata aattcttgtt tttccttgaa
    31321 gtagtactgg gaattgcttt tctaggctgc ggttactata tgatgaaaca cgtggggacc
    31381 tatcaagtct ttggtatagg ggtagcctta gttacgatca tattaggaac ttatttcatt
    31441 ttccattctg tgatcatttt ctttttatca ttactaaaga aatcagaaca gatttcattg
    31501 aaaaaattga acaatttcac attgtcacag ttaaactttc gtattcgtga atatacacaa
    31561 atgctctcaa tggtcgctat gctgtttgcc ttggcactgg gcgctttgac ggtaggatta
    31621 ggttttaaaa atgaaattgc agacttgaca gaaaacaatt ctggatatga tctagtgatg
    31681 aataatcctc gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac actttctgct
    31741 tcttatactc aaaaagaaga tgcgggaacg atttactata atgcaagtga atttgcggct
    31801 caaccattga tcatgataaa acatgaagat ggaacaccgc cgaagatcac gaaagaaaaa
    31861 gttcctgcta aaaaaatgaa tgaactgact gtacaagagg aacttcgaag ctatgaattg
    31921 ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat cagaatttga tcaactgaac
    31981 ttgccacaat ccactttaca gctagttcag gtaaaagact tccaagcaaa cctgaaacca
    32041 atcgaaacac ttgttgctca aaataaaatg aacaatccat ctttgcaaaa agtagagtac
    32101 agcaaccaga aatatgaaat gtatgaagtg tataatggtc tattctctgg ttttgaattt
    32161 atgggattct tcctaggttt agctttcttg accatgcttg ctagctgttt gatgttcaag
    32221 atcttgtcag gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa gataggaaca
    32281 cgacgcgggt tgcttaaaca atcgatccgt agagaagtag gcgtgttatt cttagcacca
    32341 ggaatcttag gtgtagtcca tgtcttattc ggattacaaa tgtttggtct attcatgcaa
    32401 aatccataca aagatatttg ggttccgttt acgatcttct tcgtattgta tttcatctac
    32461 tatgtactga cgacatggtt atatactgga atcgtattga aaaagagatt gtgaaaaagc
    32521 tgtcctgcat caagtaataa gaacagccaa gcaaaaatag ctcctcatat ttttcgtttg
    32581 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa aaagaggttg
    32641 tgacaatatt tttgtcacaa cctttttttc acggtctttt attagaatat agtaaatttt
    32701 ttctcgttac tcacggaata agctgttaag aaaacggttt ccatgtatac tggaatcagt
    32761 gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa ttttggcgaa
    32821 aactatcact tgattacgat cacgaatcaa aatgggacat ctttgtctgt cagtgatctt
    32881 ggggcacggg ttgttcattt tcaaacaata atcgataatg aaaaacgaga gttgatctta
    32941 ggttttgatg aagcaaaaga atatctagaa aaagaccctt atatcggtgc ttcaatcgga
    33001 cgtatggctg gacggattac gaatggcaca ttcaaattag atgatgaaac gttcgaagta
    33061 aaaacagacc cggacactgg tcattgttta catggtggtg cacctagctt tgaagccaag
    33121 cagtggcatt atacagtcat caatggtgaa aacgaagcat cggtcatttt ctatcagact
    33181 agcccagata tgggaaacgg atttcctgga actttagatg tagaagtacg gtatacgctg
    33241 acgaacgatg atacatggcg agtgacggtc caagggatca gtgaccgcaa aacagtattc
    33301 aatcctacaa atcatgtcta tttcaattta accggtgatc cagcacagtc aatcgacgaa
    33361 catatacttt ggttgaatag ccgattattt gctacgctca ataaagatac aacgcctaca
    33421 ggagaagtag tttcagtaag tggcacagca tttgacttcc aacgtcctaa aaaattaggt
    33481 gaggtctttg ccagtgattt tcgccaatca atgatagtgg gcggcatcga tcatccattt
    33541 ttgttgaaaa aacaatctgg tttactaaat gctgcggaat tgacaagccc agatgaaaaa
    33601 gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga tttttacagc aaattttgga
    33661 gaagatggac cggaaatgcg cgggaataaa ttagtaaatc atggcggtat cacatttgag
    33721 actcaagaac tgccaggagc ggagcgactt gaaagttttg gagatgtcac tctggaacct
    33781 aaccaagtac g
//
LOCUS       DANJJW010000020        32173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000020 DANJJW010000000
VERSION     DANJJW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(111..491)
                     /gene="yajC"
                     /locus_tag="PWS82_000914"
     CDS             complement(111..491)
                     /gene="yajC"
                     /locus_tag="PWS82_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL0906061.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(588..1733)
                     /gene="tgt"
                     /locus_tag="PWS82_000915"
     CDS             complement(588..1733)
                     /gene="tgt"
                     /locus_tag="PWS82_000915"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL0906062.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1951..2649
                     /locus_tag="PWS82_000916"
     CDS             1951..2649
                     /locus_tag="PWS82_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0906063.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2817..5456)
                     /locus_tag="PWS82_000917"
     CDS             complement(2817..5456)
                     /locus_tag="PWS82_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0906064.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6075..7106)
                     /gene="queA"
                     /locus_tag="PWS82_000918"
     CDS             complement(6075..7106)
                     /gene="queA"
                     /locus_tag="PWS82_000918"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL0906065.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7299..8474
                     /locus_tag="PWS82_000919"
     CDS             7299..8474
                     /locus_tag="PWS82_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0906066.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8478..9119
                     /locus_tag="PWS82_000920"
     CDS             8478..9119
                     /locus_tag="PWS82_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906067.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9116..10033
                     /locus_tag="PWS82_000921"
     CDS             9116..10033
                     /locus_tag="PWS82_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0906068.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10030..10701
                     /locus_tag="PWS82_000922"
     CDS             10030..10701
                     /locus_tag="PWS82_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906069.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11242..11730)
                     /locus_tag="PWS82_000923"
     CDS             complement(11242..11730)
                     /locus_tag="PWS82_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL0906070.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11931..12197)
                     /locus_tag="PWS82_000924"
     CDS             complement(11931..12197)
                     /locus_tag="PWS82_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0906071.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12197..12460)
                     /locus_tag="PWS82_000925"
     CDS             complement(12197..12460)
                     /locus_tag="PWS82_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0906072.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12936..13121
                     /locus_tag="PWS82_000926"
     CDS             12936..13121
                     /locus_tag="PWS82_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0906073.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13263..15086)
                     /locus_tag="PWS82_000927"
     CDS             complement(13263..15086)
                     /locus_tag="PWS82_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0906074.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15684..18389)
                     /locus_tag="PWS82_000928"
     CDS             complement(15684..18389)
                     /locus_tag="PWS82_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL0906075.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18695..19642)
                     /locus_tag="PWS82_000929"
     CDS             complement(18695..19642)
                     /locus_tag="PWS82_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL0906076.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19832..20101
                     /gene="rpsN"
                     /locus_tag="PWS82_000930"
     CDS             19832..20101
                     /gene="rpsN"
                     /locus_tag="PWS82_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0906077.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20277..20639)
                     /locus_tag="PWS82_000931"
     CDS             complement(20277..20639)
                     /locus_tag="PWS82_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0906078.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20650..21771)
                     /locus_tag="PWS82_000932"
     CDS             complement(20650..21771)
                     /locus_tag="PWS82_000932"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL0906079.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21787..22137)
                     /locus_tag="PWS82_000933"
     CDS             complement(21787..22137)
                     /locus_tag="PWS82_000933"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL0906080.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22267..23778)
                     /locus_tag="PWS82_000934"
     CDS             complement(22267..23778)
                     /locus_tag="PWS82_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0906081.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24100..25452)
                     /locus_tag="PWS82_000935"
     CDS             complement(24100..25452)
                     /locus_tag="PWS82_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL0906082.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25605..26681)
                     /locus_tag="PWS82_000936"
     CDS             complement(25605..26681)
                     /locus_tag="PWS82_000936"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL0906083.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26877..28622
                     /locus_tag="PWS82_000937"
     CDS             26877..28622
                     /locus_tag="PWS82_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL0906084.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28902..29339)
                     /locus_tag="PWS82_000938"
     CDS             complement(28902..29339)
                     /locus_tag="PWS82_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL0906085.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29323..30696)
                     /locus_tag="PWS82_000939"
     CDS             complement(29323..30696)
                     /locus_tag="PWS82_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906086.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31018..31506)
                     /locus_tag="PWS82_000940"
     CDS             complement(31018..31506)
                     /locus_tag="PWS82_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906087.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31528..32130)
                     /locus_tag="PWS82_000941"
     CDS             complement(31528..32130)
                     /locus_tag="PWS82_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0906088.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9196 a         6878 c         5940 g        10159 t
ORIGIN      
        1 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
       61 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      121 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      181 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      241 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      301 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      361 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      421 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      481 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      541 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      601 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      661 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      721 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      781 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      841 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
      901 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
      961 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1021 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1081 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1141 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1201 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1261 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1321 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1381 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1441 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1501 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1561 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1621 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1681 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1741 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1801 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1861 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     1921 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     1981 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2041 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2101 atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga
     2161 ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg
     2221 acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt
     2281 cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc
     2341 tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat
     2401 tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta
     2461 ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg
     2521 ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat
     2581 ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa
     2641 gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg
     2701 agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt
     2761 cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat
     2821 tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt
     2881 acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat
     2941 agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc
     3001 atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt
     3061 acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg
     3121 acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa
     3181 ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct
     3241 cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct
     3301 ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt
     3361 tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc
     3421 tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa
     3481 cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct
     3541 gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct
     3601 ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt
     3661 tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga
     3721 gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt
     3781 gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca
     3841 tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct
     3901 ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt
     3961 tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca
     4021 atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg
     4081 cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt
     4141 tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt
     4201 actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt
     4261 acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc
     4321 gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa
     4381 tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt
     4441 tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact
     4501 gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt
     4561 gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa
     4621 gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc
     4681 gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa
     4741 ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc
     4801 atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat
     4861 accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt
     4921 tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg
     4981 atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact
     5041 accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt
     5101 ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc
     5161 aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt
     5221 tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta
     5281 aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat
     5341 ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct
     5401 ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta
     5461 ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt
     5521 tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg
     5581 cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc
     5641 atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt
     5701 acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga
     5761 tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg
     5821 taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa
     5881 acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag
     5941 ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag
     6001 agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc
     6061 ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc
     6121 tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact
     6181 aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg
     6241 aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca
     6301 ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt
     6361 ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag
     6421 aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct
     6481 aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt
     6541 aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca
     6601 tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc
     6661 atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc
     6721 cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag
     6781 attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct
     6841 tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat
     6901 aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct
     6961 tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt
     7021 aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca
     7081 aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat
     7141 tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt
     7201 tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata
     7261 gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt
     7321 caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg
     7381 gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga
     7441 tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag
     7501 aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga
     7561 tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc
     7621 aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga
     7681 tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc
     7741 gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc
     7801 caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa
     7861 cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca
     7921 tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg
     7981 caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg
     8041 cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat
     8101 tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata
     8161 attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag
     8221 tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc
     8281 ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg
     8341 aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg
     8401 tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat
     8461 ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa
     8521 aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg
     8581 taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa
     8641 ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg
     8701 gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg ccgattttac
     8761 gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca
     8821 tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga
     8881 tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt
     8941 atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag
     9001 atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc
     9061 taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa
     9121 acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt
     9181 ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc
     9241 agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa
     9301 taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg
     9361 agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct
     9421 tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag
     9481 atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg
     9541 aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga
     9601 tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg
     9661 cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt
     9721 ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg
     9781 gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga
     9841 tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt
     9901 aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga
     9961 taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt
    10021 tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc
    10081 caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat
    10141 ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt
    10201 aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc
    10261 atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta
    10321 tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac
    10381 aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg
    10441 gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt
    10501 gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt
    10561 atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact
    10621 gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca
    10681 gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt
    10741 ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc
    10801 ctccttatgg gtaaagagtt gcttttctta gctatttatc cattccgtat actccaactt
    10861 catactgcca acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag
    10921 gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa
    10981 atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt
    11041 catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac
    11101 tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct
    11161 catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt
    11221 cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg
    11281 atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga
    11341 aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt
    11401 catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa
    11461 tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt
    11521 atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg
    11581 attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga catttccaat
    11641 tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga
    11701 tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg
    11761 tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa
    11821 ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct
    11881 ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa
    11941 tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact
    12001 cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca
    12061 aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt
    12121 ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg
    12181 actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac
    12241 tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg
    12301 tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat
    12361 cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat
    12421 ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg
    12481 atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc
    12541 agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt
    12601 ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac
    12661 aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa
    12721 tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac
    12781 gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc
    12841 tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg
    12901 cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa
    12961 aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg
    13021 aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa
    13081 attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat
    13141 ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg agaataagac
    13201 aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg
    13261 gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat
    13321 cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt
    13381 acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg
    13441 atatctacat aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa
    13501 aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca
    13561 tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc
    13621 aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca
    13681 taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga
    13741 tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg
    13801 tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta
    13861 atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc
    13921 cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt
    13981 tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc
    14041 ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct
    14101 tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct
    14161 aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct
    14221 ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg
    14281 ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca
    14341 gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc
    14401 ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc
    14461 ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta
    14521 tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat
    14581 aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt
    14641 gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca
    14701 gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg
    14761 gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca
    14821 tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata
    14881 acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg
    14941 acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc
    15001 aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct
    15061 actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa
    15121 gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa
    15181 gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa
    15241 acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa
    15301 taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt
    15361 ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag
    15421 ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac
    15481 caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca
    15541 gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa
    15601 aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa
    15661 cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg
    15721 aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca
    15781 acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct
    15841 gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg
    15901 aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact
    15961 aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg
    16021 atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg
    16081 acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc
    16141 attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg
    16201 tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc
    16261 cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa
    16321 agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct
    16381 agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg
    16441 tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat caagtccgca
    16501 gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg
    16561 gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat
    16621 ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg
    16681 acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt
    16741 gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc
    16801 gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca
    16861 gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct
    16921 gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc
    16981 aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg
    17041 ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat
    17101 ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca
    17161 ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt
    17221 tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct
    17281 gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt
    17341 ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc
    17401 tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag
    17461 ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg
    17521 tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca
    17581 aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt
    17641 tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt
    17701 tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc
    17761 gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg
    17821 ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt
    17881 ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc
    17941 ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca
    18001 ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa
    18061 tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct
    18121 aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct
    18181 tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca
    18241 tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc
    18301 gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat
    18361 aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa
    18421 ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt
    18481 tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga
    18541 ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta
    18601 tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg
    18661 tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt
    18721 tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag
    18781 cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc
    18841 cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt
    18901 cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg
    18961 gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa
    19021 ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg
    19081 aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga
    19141 ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta
    19201 tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa
    19261 ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta
    19321 cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta
    19381 ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc
    19441 catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac
    19501 gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga acatgtccag
    19561 aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac
    19621 cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg
    19681 cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt
    19741 tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac
    19801 gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc
    19861 aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca
    19921 aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa
    19981 tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat
    20041 aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta
    20101 atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa
    20161 aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt
    20221 ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat
    20281 ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct
    20341 gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt
    20401 ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc
    20461 ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc
    20521 gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct
    20581 tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata
    20641 ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc
    20701 atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt
    20761 tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag
    20821 ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa
    20881 accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc
    20941 tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt
    21001 gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag
    21061 tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat
    21121 tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc
    21181 gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc
    21241 tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc
    21301 tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat
    21361 cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac
    21421 agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac
    21481 agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc
    21541 agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc
    21601 tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac
    21661 tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat
    21721 agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc
    21781 ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact
    21841 gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt
    21901 taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa
    21961 agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga
    22021 ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc
    22081 gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg
    22141 cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc
    22201 acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt
    22261 ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt
    22321 accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg
    22381 ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt
    22441 gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc
    22501 agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc
    22561 ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt
    22621 ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg
    22681 acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc
    22741 atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat
    22801 acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc
    22861 tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct
    22921 cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc
    22981 tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg
    23041 tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa
    23101 tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt
    23161 cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc
    23221 tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat
    23281 tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa
    23341 tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg
    23401 tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc
    23461 tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc
    23521 ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg
    23581 atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt
    23641 ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat
    23701 aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc
    23761 tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt
    23821 ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc
    23881 acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag
    23941 gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca
    24001 acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat
    24061 tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg
    24121 ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc
    24181 ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac
    24241 aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata
    24301 aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt
    24361 taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt
    24421 tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac
    24481 atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat
    24541 agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat
    24601 cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt
    24661 ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac
    24721 atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt
    24781 cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat
    24841 ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc
    24901 gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt
    24961 ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat
    25021 cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt
    25081 ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat
    25141 tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt
    25201 caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct
    25261 ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg
    25321 cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac
    25381 attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat
    25441 tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg
    25501 gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa
    25561 tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca
    25621 aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa
    25681 tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat
    25741 cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg
    25801 caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct
    25861 ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata
    25921 tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc
    25981 cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca
    26041 acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg
    26101 ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga
    26161 taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc
    26221 ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc
    26281 atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc
    26341 aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga
    26401 aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct
    26461 tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt
    26521 tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact
    26581 tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca
    26641 tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt
    26701 ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact
    26761 tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct
    26821 agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg
    26881 cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg
    26941 tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac
    27001 taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt
    27061 ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa
    27121 cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt
    27181 tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga
    27241 acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg
    27301 atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag
    27361 ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct
    27421 taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc
    27481 gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt
    27541 ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg
    27601 gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga
    27661 aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc
    27721 atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa
    27781 aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat
    27841 ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa
    27901 aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg
    27961 tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga
    28021 caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat
    28081 tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc
    28141 ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt
    28201 ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag
    28261 aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga
    28321 tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt
    28381 tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg
    28441 ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac
    28501 ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat
    28561 tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt
    28621 agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat
    28681 tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac
    28741 cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg
    28801 aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata
    28861 aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt
    28921 aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt
    28981 atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc
    29041 gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt
    29101 gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt
    29161 ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat
    29221 tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct
    29281 cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt
    29341 ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac
    29401 attgatccat tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt
    29461 cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag
    29521 cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac
    29581 gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct
    29641 gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat
    29701 caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt
    29761 taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg
    29821 caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct
    29881 aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct
    29941 tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag
    30001 caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct
    30061 caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag
    30121 atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta
    30181 ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg
    30241 caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc
    30301 atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac
    30361 aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa
    30421 tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa
    30481 cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa
    30541 ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa
    30601 aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt
    30661 aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt
    30721 ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata
    30781 tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac
    30841 ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc
    30901 ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg
    30961 taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta
    31021 tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat
    31081 aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc
    31141 ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca
    31201 gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact
    31261 taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct
    31321 ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg
    31381 ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg
    31441 ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt
    31501 ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa
    31561 gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat
    31621 aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc
    31681 aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag
    31741 caattcatca aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc
    31801 atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata
    31861 taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg
    31921 caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt
    31981 gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata
    32041 agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca
    32101 tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt
    32161 aaaactgcct cct
//
LOCUS       DANJJW010000021        30821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000021 DANJJW010000000
VERSION     DANJJW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..828
                     /locus_tag="PWS82_000942"
     CDS             <1..828
                     /locus_tag="PWS82_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0906089.1"
                     /translation="FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGW
                     TPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSI
                     HEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKK
                     LNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGL
                     LRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            977..1315
                     /locus_tag="PWS82_000943"
     CDS             977..1315
                     /locus_tag="PWS82_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906090.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1520..2095)
                     /locus_tag="PWS82_000944"
     CDS             complement(1520..2095)
                     /locus_tag="PWS82_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL0906091.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2827..3408)
                     /locus_tag="PWS82_000945"
     CDS             complement(2827..3408)
                     /locus_tag="PWS82_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0906092.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3591..4217)
                     /locus_tag="PWS82_000946"
     CDS             complement(3591..4217)
                     /locus_tag="PWS82_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0906093.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4239..5570)
                     /locus_tag="PWS82_000947"
     CDS             complement(4239..5570)
                     /locus_tag="PWS82_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL0906094.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5725..6126)
                     /locus_tag="PWS82_000948"
     CDS             complement(5725..6126)
                     /locus_tag="PWS82_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0906095.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6212..6418)
                     /locus_tag="PWS82_000949"
     CDS             complement(6212..6418)
                     /locus_tag="PWS82_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906096.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6513..8015)
                     /locus_tag="PWS82_000950"
     CDS             complement(6513..8015)
                     /locus_tag="PWS82_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL0906097.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8033..8488)
                     /gene="argR"
                     /locus_tag="PWS82_000951"
     CDS             complement(8033..8488)
                     /gene="argR"
                     /locus_tag="PWS82_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0906098.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8586..9203)
                     /locus_tag="PWS82_000952"
     CDS             complement(8586..9203)
                     /locus_tag="PWS82_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL0906099.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9424..10797
                     /locus_tag="PWS82_000953"
     CDS             9424..10797
                     /locus_tag="PWS82_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906100.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10872..11984
                     /locus_tag="PWS82_000954"
     CDS             10872..11984
                     /locus_tag="PWS82_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL0906101.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12056..13693)
                     /locus_tag="PWS82_000955"
     CDS             complement(12056..13693)
                     /locus_tag="PWS82_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL0906102.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13861..14469)
                     /locus_tag="PWS82_000956"
     CDS             complement(13861..14469)
                     /locus_tag="PWS82_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0906103.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14853..16004)
                     /gene="queG"
                     /locus_tag="PWS82_000957"
     CDS             complement(14853..16004)
                     /gene="queG"
                     /locus_tag="PWS82_000957"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL0906104.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16023..16775)
                     /locus_tag="PWS82_000958"
     CDS             complement(16023..16775)
                     /locus_tag="PWS82_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906105.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16916..17866)
                     /locus_tag="PWS82_000959"
     CDS             complement(16916..17866)
                     /locus_tag="PWS82_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0906106.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17867..18706)
                     /locus_tag="PWS82_000960"
     CDS             complement(17867..18706)
                     /locus_tag="PWS82_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0906107.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18703..19449)
                     /locus_tag="PWS82_000961"
     CDS             complement(18703..19449)
                     /locus_tag="PWS82_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0906108.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19687..21057
                     /locus_tag="PWS82_000962"
     CDS             19687..21057
                     /locus_tag="PWS82_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0906109.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21112..22008)
                     /locus_tag="PWS82_000963"
     CDS             complement(21112..22008)
                     /locus_tag="PWS82_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0906110.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22122..22292)
                     /locus_tag="PWS82_000964"
     CDS             complement(22122..22292)
                     /locus_tag="PWS82_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906111.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22289..22966)
                     /locus_tag="PWS82_000965"
     CDS             complement(22289..22966)
                     /locus_tag="PWS82_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0906112.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23057..23962)
                     /locus_tag="PWS82_000966"
     CDS             complement(23057..23962)
                     /locus_tag="PWS82_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0906113.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24137..25591
                     /locus_tag="PWS82_000967"
     CDS             24137..25591
                     /locus_tag="PWS82_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906114.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            25607..26638
                     /locus_tag="PWS82_000968"
     CDS             25607..26638
                     /locus_tag="PWS82_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0906115.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26645..27832
                     /locus_tag="PWS82_000969"
     CDS             26645..27832
                     /locus_tag="PWS82_000969"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL0906116.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27988..29370
                     /locus_tag="PWS82_000970"
     CDS             27988..29370
                     /locus_tag="PWS82_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0906117.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29342..29728
                     /locus_tag="PWS82_000971"
     CDS             29342..29728
                     /locus_tag="PWS82_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906118.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29928..30596)
                     /locus_tag="PWS82_000972"
     CDS             complement(29928..30596)
                     /locus_tag="PWS82_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL0906119.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8690 a         6279 c         5644 g        10208 t
ORIGIN      
        1 ttcaagcaag gaaaatcagc cttagaatat tatcaacagt ataagaaaaa caaatcaaac
       61 tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca aagaaaggtt
      121 gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat tagttgttct
      181 gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga cttaccgatg
      241 aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac tttccgccgc
      301 tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg tcaccttgaa
      361 ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt tgaacgatta
      421 tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat tgaaaaaaaa
      481 ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac ttttgattgt
      541 ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc catttatttc
      601 gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg attgttacgt
      661 agagatggtt tattgaaatc tatggatttc aattcagtag atgaattttt tattcaatct
      721 gtcgcatcta aacgaaataa tattcctaga aaatcactgg attatcgaac acctttggaa
      781 gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca attaagaaac
      841 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
      901 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      961 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
     1021 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
     1081 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
     1141 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
     1201 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
     1261 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
     1321 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
     1381 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
     1441 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
     1501 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
     1561 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
     1621 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
     1681 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
     1741 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
     1801 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1861 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1921 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1981 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     2041 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     2101 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     2161 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     2221 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     2281 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     2341 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     2401 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     2461 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     2521 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     2581 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     2641 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     2701 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     2761 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     2821 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2881 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2941 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     3001 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     3061 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     3121 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     3181 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     3241 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     3301 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     3361 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     3421 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     3481 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     3541 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     3601 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     3661 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     3721 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     3781 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3841 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3901 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3961 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     4021 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     4081 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     4141 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     4201 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     4261 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     4321 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     4381 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     4441 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     4501 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     4561 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     4621 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     4681 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     4741 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     4801 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4861 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4921 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4981 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     5041 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     5101 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     5161 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     5221 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     5281 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     5341 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     5401 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     5461 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     5521 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     5581 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     5641 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     5701 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     5761 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     5821 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5881 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5941 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     6001 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     6061 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     6121 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     6181 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     6241 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     6301 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     6361 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     6421 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     6481 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     6541 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     6601 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     6661 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     6721 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     6781 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6841 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6901 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6961 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     7021 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     7081 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     7141 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     7201 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     7261 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     7321 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     7381 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     7441 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     7501 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     7561 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     7621 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     7681 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     7741 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     7801 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7861 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7921 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7981 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     8041 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     8101 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     8161 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     8221 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     8281 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     8341 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     8401 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     8461 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     8521 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     8581 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     8641 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     8701 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     8761 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     8821 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8881 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8941 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     9001 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     9061 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     9121 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     9181 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     9241 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     9301 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     9361 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     9421 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     9481 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     9541 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     9601 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     9661 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     9721 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     9781 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9841 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9901 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9961 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
    10021 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
    10081 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
    10141 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
    10201 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
    10261 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
    10321 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
    10381 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
    10441 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
    10501 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
    10561 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
    10621 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
    10681 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
    10741 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
    10801 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10861 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10921 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10981 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    11041 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    11101 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    11161 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    11221 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    11281 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    11341 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    11401 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    11461 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    11521 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    11581 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    11641 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    11701 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    11761 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    11821 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11881 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11941 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    12001 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    12061 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    12121 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    12181 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    12241 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    12301 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    12361 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    12421 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    12481 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    12541 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    12601 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    12661 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    12721 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    12781 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12841 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12901 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12961 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    13021 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    13081 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    13141 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    13201 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    13261 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    13321 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    13381 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    13441 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    13501 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    13561 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    13621 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    13681 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    13741 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    13801 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13861 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13921 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13981 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    14041 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    14101 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    14161 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    14221 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    14281 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    14341 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    14401 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    14461 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    14521 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    14581 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    14641 ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat
    14701 tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca
    14761 aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg
    14821 gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg caataatttc
    14881 tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct
    14941 aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct
    15001 gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt
    15061 cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc
    15121 catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt
    15181 ggctttaatt ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa
    15241 tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt
    15301 cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa
    15361 catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg
    15421 cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta
    15481 gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt
    15541 cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta
    15601 tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg
    15661 atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa
    15721 gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta
    15781 ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa
    15841 agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct
    15901 tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt
    15961 cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc
    16021 tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc
    16081 tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa
    16141 taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact
    16201 ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga
    16261 tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc
    16321 gggattttcc caggcctagc aatttctact agacgattat aaaacatatt cattaatttg
    16381 ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat tactccttct
    16441 gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta
    16501 actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta
    16561 ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct
    16621 ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat
    16681 tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct
    16741 ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta
    16801 actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt
    16861 tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc
    16921 tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat
    16981 agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga
    17041 ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca
    17101 cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag
    17161 gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc
    17221 gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag
    17281 aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg
    17341 catttttttt atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt
    17401 cctccacata ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat
    17461 gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt
    17521 ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa
    17581 gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta
    17641 gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat
    17701 ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact
    17761 tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca
    17821 agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt
    17881 tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac
    17941 atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga
    18001 caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg
    18061 ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta
    18121 ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca
    18181 gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt
    18241 ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca
    18301 aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac
    18361 tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa
    18421 tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta
    18481 gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta
    18541 cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa
    18601 taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt
    18661 gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc
    18721 ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac
    18781 ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata
    18841 ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa
    18901 ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc
    18961 atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag
    19021 ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt
    19081 caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga
    19141 tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc
    19201 acttcgctgt tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa
    19261 ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt
    19321 tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat
    19381 agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt
    19441 acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta
    19501 actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg
    19561 cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat
    19621 gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt
    19681 ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc
    19741 gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg
    19801 acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg
    19861 cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg
    19921 agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat
    19981 attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct
    20041 gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt
    20101 atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg
    20161 atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt
    20221 caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa
    20281 ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga
    20341 atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact
    20401 atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg
    20461 tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt
    20521 acatttgctg caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat
    20581 tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct
    20641 aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca
    20701 ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca
    20761 gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta
    20821 gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat
    20881 ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc
    20941 gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt
    21001 ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa
    21061 aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa
    21121 ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa
    21181 gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa
    21241 aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt
    21301 tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga
    21361 atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg
    21421 caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat
    21481 caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt
    21541 tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga
    21601 taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc
    21661 aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg
    21721 caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc
    21781 acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc
    21841 ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa
    21901 cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct
    21961 tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac
    22021 caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg
    22081 tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc
    22141 caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc
    22201 agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca
    22261 tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg
    22321 taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt
    22381 gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt
    22441 caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta
    22501 gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa
    22561 gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg
    22621 tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga
    22681 ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa
    22741 tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat
    22801 gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt
    22861 ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta
    22921 aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt
    22981 tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc
    23041 attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt
    23101 tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg
    23161 tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc
    23221 ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc
    23281 gattgatccg taccccgatc aaatattcct tgatactggt acccatatga tttttgaata
    23341 ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt
    23401 tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct
    23461 tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg
    23521 ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta
    23581 aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga
    23641 aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca
    23701 tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca
    23761 gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca
    23821 tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg
    23881 ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt
    23941 ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa
    24001 aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag
    24061 tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag
    24121 gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc
    24181 ttttggacac gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag
    24241 ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag
    24301 cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat
    24361 cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt
    24421 tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac
    24481 gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt
    24541 taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga
    24601 aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt
    24661 cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc
    24721 ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat
    24781 cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg
    24841 gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc
    24901 ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa
    24961 atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg
    25021 tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta
    25081 ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag
    25141 tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt
    25201 atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg
    25261 taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc
    25321 taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc
    25381 tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc
    25441 tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca
    25501 tgcacaaatc gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga
    25561 acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga
    25621 aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact
    25681 ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt
    25741 tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat
    25801 cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca
    25861 attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt
    25921 aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac
    25981 tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac
    26041 tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt
    26101 attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga
    26161 tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc
    26221 tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt
    26281 agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc
    26341 atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa
    26401 aagaatccaa gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt
    26461 cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt
    26521 tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa
    26581 agctggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact
    26641 catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa
    26701 aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa
    26761 tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg
    26821 agacgataat aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt
    26881 tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat
    26941 ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga
    27001 aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg
    27061 tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg
    27121 taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat
    27181 cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat
    27241 ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga
    27301 attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt
    27361 gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac
    27421 tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa
    27481 tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca
    27541 actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt
    27601 agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg
    27661 agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa
    27721 aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc
    27781 acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa
    27841 tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg
    27901 agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt
    27961 tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat
    28021 gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta
    28081 gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg
    28141 tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa
    28201 aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt
    28261 gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat
    28321 tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc
    28381 ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct
    28441 aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc
    28501 gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg
    28561 tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact
    28621 gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct
    28681 gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt
    28741 actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca
    28801 ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa
    28861 gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct
    28921 gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca
    28981 aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat
    29041 ctagcagatg ctttattgac cattttccac cagcattacc actaccagga tattcggcgt
    29101 gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa
    29161 gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa
    29221 aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc
    29281 gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa
    29341 gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg
    29401 attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga
    29461 agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag
    29521 tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga
    29581 tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga
    29641 ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa
    29701 ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa
    29761 ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt
    29821 cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt
    29881 agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt
    29941 cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac
    30001 cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata
    30061 agccatgctt cagtcaattg attggactga agcactttat gctgaatttg cccttcatct
    30121 acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca
    30181 gcattctcta ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag
    30241 attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg
    30301 ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt
    30361 tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca
    30421 tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc
    30481 gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc
    30541 actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt
    30601 tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat
    30661 atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc
    30721 aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat
    30781 atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DANJJW010000022        28142 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000022 DANJJW010000000
VERSION     DANJJW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(205..1104)
                     /locus_tag="PWS82_000973"
     CDS             complement(205..1104)
                     /locus_tag="PWS82_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL0906120.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="PWS82_000974"
     CDS             complement(1114..1470)
                     /locus_tag="PWS82_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0906121.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="PWS82_000975"
     CDS             1825..2121
                     /locus_tag="PWS82_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL0906122.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="PWS82_000976"
     CDS             complement(2765..3718)
                     /locus_tag="PWS82_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0906123.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="PWS82_000977"
     CDS             complement(3741..4496)
                     /locus_tag="PWS82_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0906124.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="PWS82_000978"
     CDS             complement(4493..5452)
                     /locus_tag="PWS82_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0906125.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="PWS82_000979"
     CDS             complement(5445..6395)
                     /locus_tag="PWS82_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0906126.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="PWS82_000980"
     CDS             6545..6997
                     /locus_tag="PWS82_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL0906127.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="PWS82_000981"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="PWS82_000981"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL0906128.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="PWS82_000982"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="PWS82_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL0906129.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="PWS82_000983"
     CDS             complement(10549..11358)
                     /locus_tag="PWS82_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0906130.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="PWS82_000984"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="PWS82_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL0906131.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="PWS82_000985"
     CDS             complement(14968..15654)
                     /locus_tag="PWS82_000985"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL0906132.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="PWS82_000986"
     CDS             complement(16002..17783)
                     /locus_tag="PWS82_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0906133.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="PWS82_000987"
     CDS             complement(17809..18723)
                     /locus_tag="PWS82_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906134.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="PWS82_000988"
     CDS             complement(18750..19712)
                     /locus_tag="PWS82_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906135.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="PWS82_000989"
     CDS             complement(19712..20656)
                     /locus_tag="PWS82_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906136.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="PWS82_000990"
     CDS             complement(20657..21661)
                     /locus_tag="PWS82_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906137.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="PWS82_000991"
     CDS             complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="PWS82_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0906138.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="PWS82_000992"
     CDS             complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="PWS82_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL0906139.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23343..25379)
                     /gene="recG"
                     /locus_tag="PWS82_000993"
     CDS             complement(23343..25379)
                     /gene="recG"
                     /locus_tag="PWS82_000993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL0906140.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25496..27178)
                     /locus_tag="PWS82_000994"
     CDS             complement(25496..27178)
                     /locus_tag="PWS82_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL0906141.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27230..27592)
                     /locus_tag="PWS82_000995"
     CDS             complement(27230..27592)
                     /locus_tag="PWS82_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0906142.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27862..28050
                     /locus_tag="PWS82_000996"
     CDS             27862..28050
                     /locus_tag="PWS82_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL0906143.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7782 a         6085 c         4955 g         9320 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct gcatgcagct
    21901 ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata taatctactg
    21961 cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta ccgaattcat
    22021 cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa tcatctttga
    22081 tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa ataattttcg
    22141 ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat catacacttg
    22201 tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc accttttttt
    22261 caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc aaattgttcg
    22321 actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat cgtatagcga
    22381 acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa
    22441 acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa gccgtctttt
    22501 aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga ttttaataga
    22561 ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca
    22621 tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac gtttccaata
    22681 aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac ttcactacct
    22741 tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat acctcggact
    22801 ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg tttgttttct
    22861 gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac tactggcaag
    22921 gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa caaccctgct
    22981 gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc gtttttcact
    23041 gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt tactggctca
    23101 tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc atttgttaag
    23161 tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc
    23221 tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc cattgcatct
    23281 acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta tagcacagtc
    23341 tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc taaaggctga
    23401 tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc ttctctggct
    23461 acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg cagaccagat
    23521 tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc gcttagtaca
    23581 aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc atttttagga
    23641 ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc acgaagttgg
    23701 tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt cgcgttcggc
    23761 acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg gttgtctttg
    23821 aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa caagccaact
    23881 tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc attttttaca
    23941 tctaatgctt ctgattcttc aatcagcgga cagatgacat acatttgatg tcctcttgcc
    24001 agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac ccagcgtgtt
    24061 tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat ctcaccatat
    24121 gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac atctggtcgc
    24181 cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg ctcatccgta
    24241 ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg tgtgccgatc
    24301 acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc ttttgctttc
    24361 gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg ttgcaagctt
    24421 tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg gaaacctgct
    24481 gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc tacatctccc
    24541 tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc attcgttaca
    24601 cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt caatttttgg
    24661 acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt caatccttga
    24721 agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc ttgatggttt
    24781 tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc aagcagtcga
    24841 tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta ttcttgaaac
    24901 ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac gtgatatatc
    24961 ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa ggctttacgc
    25021 tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt ttcttttaga
    25081 taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat acgaaattgc
    25141 aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac gattcctttc
    25201 aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat atcttcgtag
    25261 cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct ggcaagacta
    25321 gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa agaacgcata
    25381 aatttttcct ccctccttta acaaaaaaag gacttggtca gcgatcatac ctgatcacta
    25441 accgaaggcc ttttatgaat gaatactgtg ttagaaagat attctgacac agaaattatt
    25501 cagcagaaaa tagataagga tagacaggct gatctccttg gtggatctcg atttccaaat
    25561 caggatgttt ttctgttagt ttctcagcaa attcatttgc ttgttcaact gttccttcat
    25621 cacctgccaa gatcgttacg atctctgtgt cctcattgat catttgttct agtgtactta
    25681 acgctgttgc tgtgatatct gggttagaga tcaggatttt tccatcgatc atgcccaaaa
    25741 agtctccttc tttgatctct acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt
    25801 gaccgctcac aacgctagac aacatttctg tcatcgtttc tttgttttct tctaaagaag
    25861 cttgatcatt gaatgaaagc atagctgtca atccttggga aatcgttctt gaaggaacaa
    25921 ctgctactgg gatatctgct acttctgctg cttgatcagc tgccatgaag atgttcttat
    25981 tgtttggcaa gacaatcact ttatctgcgt ttacttcttg aatcgcttta acaatgtctt
    26041 ccgtacttgg attcattgtt tgtccaccac tgataacata attagcgcct aaactttcaa
    26101 acagctcttg gacgccttta ccagcagcga tcgctacgat accatacggg atacgtggta
    26161 tttctttttt cgcaaactcg ttcacttctt catcgtgttc caaaatcgtc tcatgctgca
    26221 agcgcatgtt gtcgactttg attttgacta atgaaccgaa tttttgtcca taattcaaga
    26281 cttctcctgg gttttctgta tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa
    26341 gtaaagaatc tcctatacca tctaaatagt tacggaattc ttcgtaatcg aatgtactat
    26401 cgactgtagg tccttctccg atttttacca tgatttctgt acagtaacca aaatggatat
    26461 cttctgtcgc catttgacct tggacactgc gatgatgttc tgcattgacc atatcatcca
    26521 tttctgctgg agaaggttca tatgtttcat ccgcttggaa ttctccattc aatgcactta
    26581 aaaagccctc ataaacaaac agcagtcctt gccctccact gtctacgaca ccgacttctt
    26641 ttaggactgg aagcaagtct ggagttttag ctaaagcacg ctttgctcct tttacaacag
    26701 ctgtcattac ttcaacgaca tcatcagtac tttttgcttt cttttctcca gctttcgctg
    26761 cttctctagc tactgtcaaa atcgttcctt ctactggttt cattactgct ttatacgcag
    26821 tttcaactcc atgagtgaag gctgcagcaa gatcagaagc atttaatgag acaacatctg
    26881 gaatttgctt agaaaatcca cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg
    26941 cacccatcag caatcctttt gaaagcattg ctgctaattc tcctactttt tctgaggttg
    27001 aatctactac tgccttagct ccactagtca tagatagatt catatttgtt cccgtatcgc
    27061 catctggcac agggaataca tttaatgaat tgacgtattc tgcatttact tgcagacgat
    27121 tcgcacctgc ctggaccatt tcctggaact ggcttgcact tatttctgtt acactcacct
    27181 agataatcct ccttcagatc gactgctgag ttaccagcca gtctcctgcc tagtctggca
    27241 gtacacgaac accttgtaca aagacattaa ctgaattggc agttacgcct aataatgcct
    27301 ctagattgta tttcactttt tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat
    27361 agctgataat cgtataaaca tctacagcaa taccattatc ttcttggcgg acaactactc
    27421 ctcttgagta attttcttta cgcaaaatct cagtaatatt atctttaatt tgatttttgc
    27481 tggccattcc tacaacaccg aacacatctg tcgcggcgcc accaacaact gttgcaatta
    27541 catcattggt aatttcaatg gttccagctg acgtttgaat ttttacagcc atgaatgagc
    27601 ctccttaacg ggtaaaaaac tcccattttt cttctatcat attttatcat agcgcaactt
    27661 gaaataaaag gcagttaccc aaagtactca aaaaccttgc aaaatttcag gtataacctt
    27721 gtttaattta caattattct tctcaattgt caattgtatc tatgaaaatg tacttgcaaa
    27781 ttcccattct atatgatatg ttagtctggt atgagctgaa ttattctatt gctccaaatc
    27841 aaggcaaagg aggaaattac catggcaaaa gtttgttact ttacaggtcg caagacaaaa
    27901 agtggaaaca accgttcaca cgctatgaac tcaacaaaac gtactgttaa acctaactta
    27961 caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct
    28021 ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata cttggaccgt tcagatagtg
    28081 aacggttttt tatttaataa aaatttttaa gggtgggact tttgattcct accctttttt
    28141 tg
//
LOCUS       DANJJW010000023        27958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000023 DANJJW010000000
VERSION     DANJJW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="PWS82_000997"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="PWS82_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL0906144.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="PWS82_000998"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="PWS82_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL0906145.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="PWS82_000999"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="PWS82_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL0906146.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="PWS82_001000"
     CDS             1642..2274
                     /locus_tag="PWS82_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0906147.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="PWS82_001001"
     CDS             complement(2276..3181)
                     /locus_tag="PWS82_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0906148.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="PWS82_001002"
     CDS             complement(3266..3925)
                     /locus_tag="PWS82_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0906149.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="PWS82_001003"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="PWS82_001003"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL0906150.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="PWS82_001004"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="PWS82_001004"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL0906151.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="PWS82_001005"
     CDS             6168..7253
                     /locus_tag="PWS82_001005"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL0906152.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="PWS82_001006"
     CDS             7250..8314
                     /locus_tag="PWS82_001006"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL0906153.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="PWS82_001007"
     CDS             complement(8563..9366)
                     /locus_tag="PWS82_001007"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL0906154.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="PWS82_001008"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="PWS82_001008"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL0906155.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="PWS82_001009"
     CDS             complement(10244..11251)
                     /locus_tag="PWS82_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0906156.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="PWS82_001010"
     CDS             complement(11258..11902)
                     /locus_tag="PWS82_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL0906157.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="PWS82_001011"
     CDS             complement(12002..12760)
                     /locus_tag="PWS82_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL0906158.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="PWS82_001012"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="PWS82_001012"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL0906159.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="PWS82_001013"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="PWS82_001013"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL0906160.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="PWS82_001014"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="PWS82_001014"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL0906161.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="PWS82_001015"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="PWS82_001015"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL0906162.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="PWS82_001016"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="PWS82_001016"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL0906163.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="PWS82_001017"
     CDS             18158..19027
                     /locus_tag="PWS82_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL0906164.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="PWS82_001018"
     CDS             complement(19153..20052)
                     /locus_tag="PWS82_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906165.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="PWS82_001019"
     CDS             complement(20217..21041)
                     /locus_tag="PWS82_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0906166.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="PWS82_001020"
     CDS             complement(21052..22347)
                     /locus_tag="PWS82_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL0906167.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="PWS82_001021"
     CDS             complement(22363..22842)
                     /locus_tag="PWS82_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL0906168.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="PWS82_001022"
     CDS             complement(22848..23876)
                     /locus_tag="PWS82_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL0906169.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="PWS82_001023"
     CDS             complement(23893..24999)
                     /locus_tag="PWS82_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0906170.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="PWS82_001024"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="PWS82_001024"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL0906171.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7884 a         6132 c         5041 g         8901 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttattt
//
LOCUS       DANJJW010000024        27909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000024 DANJJW010000000
VERSION     DANJJW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(414..1358)
                     /locus_tag="PWS82_001025"
     CDS             complement(414..1358)
                     /locus_tag="PWS82_001025"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0906172.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1654..2598)
                     /locus_tag="PWS82_001026"
     CDS             complement(1654..2598)
                     /locus_tag="PWS82_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906173.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2819..4033
                     /locus_tag="PWS82_001027"
     CDS             2819..4033
                     /locus_tag="PWS82_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906174.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4055..6070
                     /locus_tag="PWS82_001028"
     CDS             4055..6070
                     /locus_tag="PWS82_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL0906175.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6153..7526)
                     /locus_tag="PWS82_001029"
     CDS             complement(6153..7526)
                     /locus_tag="PWS82_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0906176.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7552..7836)
                     /locus_tag="PWS82_001030"
     CDS             complement(7552..7836)
                     /locus_tag="PWS82_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0906177.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7838..9853)
                     /locus_tag="PWS82_001031"
     CDS             complement(7838..9853)
                     /locus_tag="PWS82_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0906178.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10032..10337)
                     /locus_tag="PWS82_001032"
     CDS             complement(10032..10337)
                     /locus_tag="PWS82_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL0906179.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10426..11457)
                     /locus_tag="PWS82_001033"
     CDS             complement(10426..11457)
                     /locus_tag="PWS82_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0906180.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11664..12539
                     /locus_tag="PWS82_001034"
     CDS             11664..12539
                     /locus_tag="PWS82_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0906181.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12691..13533)
                     /locus_tag="PWS82_001035"
     CDS             complement(12691..13533)
                     /locus_tag="PWS82_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0906182.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13625..15502)
                     /locus_tag="PWS82_001036"
     CDS             complement(13625..15502)
                     /locus_tag="PWS82_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0906183.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15499..16920)
                     /locus_tag="PWS82_001037"
     CDS             complement(15499..16920)
                     /locus_tag="PWS82_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0906184.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16923..20312)
                     /locus_tag="PWS82_001038"
     CDS             complement(16923..20312)
                     /locus_tag="PWS82_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL0906185.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20569..21960
                     /locus_tag="PWS82_001039"
     CDS             20569..21960
                     /locus_tag="PWS82_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906186.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22033..22680)
                     /locus_tag="PWS82_001040"
     CDS             complement(22033..22680)
                     /locus_tag="PWS82_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0906187.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22688..23374)
                     /locus_tag="PWS82_001041"
     CDS             complement(22688..23374)
                     /locus_tag="PWS82_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906188.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23380..24453)
                     /locus_tag="PWS82_001042"
     CDS             complement(23380..24453)
                     /locus_tag="PWS82_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906189.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24675..25349
                     /locus_tag="PWS82_001043"
     CDS             24675..25349
                     /locus_tag="PWS82_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906190.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25354..26694
                     /locus_tag="PWS82_001044"
     CDS             25354..26694
                     /locus_tag="PWS82_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0906191.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26843..27829)
                     /locus_tag="PWS82_001045"
     CDS             complement(26843..27829)
                     /locus_tag="PWS82_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL0906192.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8136 a         5271 c         5057 g         9445 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      421 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      481 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      541 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      601 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      661 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      721 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      781 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      841 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      901 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      961 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1021 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1081 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1141 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1201 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1261 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1321 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1381 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1441 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1501 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1561 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1621 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1681 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1741 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1801 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1861 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1921 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1981 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2041 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2101 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2161 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2221 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2281 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2341 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2401 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2461 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2521 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2581 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2641 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2701 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2761 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2821 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2881 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2941 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     3001 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     3061 tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     3121 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3181 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3241 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3301 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3361 tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa
     3421 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3481 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3541 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3601 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3661 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3721 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3781 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3841 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3901 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3961 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     4021 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     4081 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     4141 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4201 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4261 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4321 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4381 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4441 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4501 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4561 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4621 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4681 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4741 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4801 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4861 atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4921 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4981 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     5041 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     5101 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     5161 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5221 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5281 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5341 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5401 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5461 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5521 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5581 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5641 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5701 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5761 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5821 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5881 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5941 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     6001 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     6061 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     6121 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6181 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6241 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6301 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6361 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6421 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6481 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6541 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6601 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6661 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6721 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6781 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6841 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6901 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6961 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     7021 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     7081 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     7141 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7201 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7261 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7321 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7381 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7441 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7501 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7561 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7621 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7681 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7741 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7801 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7861 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7921 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7981 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     8041 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     8101 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     8161 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8221 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8281 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8341 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8401 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8461 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8521 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8581 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8641 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8701 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8761 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8821 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8881 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8941 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     9001 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     9061 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     9121 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9181 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9241 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9301 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9361 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9421 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9481 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9541 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9601 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9661 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9721 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9781 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9841 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9901 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9961 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
    10021 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
    10081 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
    10141 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10201 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10261 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10321 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10381 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10441 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10501 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10561 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10621 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10681 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10741 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10801 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10861 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10921 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10981 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    11041 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    11101 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    11161 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11221 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11281 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11341 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11401 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11461 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11521 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11581 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11641 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11701 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11761 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11821 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11881 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11941 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    12001 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    12061 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    12121 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12181 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12241 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12301 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12361 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12421 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12481 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12541 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12601 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12661 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12721 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12781 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12841 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12901 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12961 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    13021 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    13081 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    13141 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13201 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13261 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13321 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13381 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13441 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13501 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13561 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13621 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13681 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13741 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13801 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13861 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13921 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13981 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    14041 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    14101 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    14161 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    14221 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    14281 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    14341 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    14401 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14461 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14521 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14581 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14641 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14701 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14761 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14821 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14881 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14941 cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    15001 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    15061 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    15121 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15181 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15241 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15301 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15361 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15421 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15481 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15541 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15601 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15661 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15721 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15781 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15841 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15901 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15961 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    16021 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    16081 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    16141 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16201 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16261 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16321 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16381 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16441 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16501 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16561 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16621 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16681 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16741 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16801 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16861 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16921 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16981 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    17041 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    17101 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    17161 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17221 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17281 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17341 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17401 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17461 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17521 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17581 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17641 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17701 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17761 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17821 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17881 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17941 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    18001 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    18061 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    18121 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18181 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    18241 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18301 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18361 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18421 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18481 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18541 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18601 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18661 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18721 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18781 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18841 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18901 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18961 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    19021 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    19081 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    19141 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19201 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19261 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19321 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19381 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19441 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19501 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19561 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19621 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19681 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19741 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19801 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19861 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19921 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19981 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    20041 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    20101 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    20161 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20221 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20281 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20341 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20401 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20461 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20521 gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat ggaaaaaatc
    20581 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20641 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20701 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20761 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20821 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20881 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20941 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    21001 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    21061 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    21121 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21181 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21241 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21301 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21361 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21421 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21481 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21541 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21601 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21661 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21721 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21781 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21841 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21901 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21961 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    22021 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    22081 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    22141 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22201 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22261 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22321 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22381 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22441 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22501 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22561 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22621 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22681 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22741 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22801 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22861 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22921 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22981 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    23041 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    23101 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    23161 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23221 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23281 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23341 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23401 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23461 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23521 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23581 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23641 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23701 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23761 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23821 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23881 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23941 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    24001 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    24061 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    24121 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24181 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24241 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24301 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24361 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24421 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24481 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24541 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24601 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24661 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24721 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24781 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24841 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24901 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24961 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    25021 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    25081 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    25141 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25201 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25261 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25321 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25381 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25441 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25501 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25561 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25621 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25681 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25741 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25801 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25861 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25921 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25981 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    26041 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    26101 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    26161 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26221 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26281 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26341 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26401 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26461 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26521 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26581 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26641 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26701 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26761 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26821 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26881 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26941 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    27001 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    27061 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    27121 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27181 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27241 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27301 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27361 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27421 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27481 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27541 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27601 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27661 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27721 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27781 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27841 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27901 ttgttttct
//
LOCUS       DANJJW010000025        27560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000025 DANJJW010000000
VERSION     DANJJW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(407..766)
                     /locus_tag="PWS82_001046"
     CDS             complement(407..766)
                     /locus_tag="PWS82_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL0906193.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="PWS82_001047"
     CDS             complement(796..1137)
                     /locus_tag="PWS82_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906194.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="PWS82_001048"
     CDS             complement(1134..1808)
                     /locus_tag="PWS82_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0906195.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWS82_001049"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWS82_001049"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL0906196.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="PWS82_001050"
     CDS             complement(4322..5512)
                     /locus_tag="PWS82_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0906197.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="PWS82_001051"
     CDS             complement(5533..6060)
                     /locus_tag="PWS82_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL0906198.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="PWS82_001052"
     CDS             complement(6107..8896)
                     /locus_tag="PWS82_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0906199.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="PWS82_001053"
     CDS             complement(9043..9243)
                     /locus_tag="PWS82_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0906200.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="PWS82_001054"
     CDS             complement(9696..10016)
                     /locus_tag="PWS82_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906201.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="PWS82_001055"
     CDS             complement(10519..11637)
                     /locus_tag="PWS82_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906202.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="PWS82_001056"
     CDS             12108..13415
                     /locus_tag="PWS82_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL0906203.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="PWS82_001057"
     CDS             13536..14735
                     /locus_tag="PWS82_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0906204.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="PWS82_001058"
     CDS             complement(14762..16030)
                     /locus_tag="PWS82_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL0906205.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="PWS82_001059"
     CDS             complement(16200..16841)
                     /locus_tag="PWS82_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL0906206.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="PWS82_001060"
     CDS             complement(17250..17753)
                     /locus_tag="PWS82_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0906207.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="PWS82_001061"
     CDS             complement(17876..18640)
                     /locus_tag="PWS82_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL0906208.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="PWS82_001062"
     CDS             18748..19023
                     /locus_tag="PWS82_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL0906209.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="PWS82_001063"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="PWS82_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0906210.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="PWS82_001064"
     CDS             complement(20579..20980)
                     /locus_tag="PWS82_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0906211.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="PWS82_001065"
     CDS             complement(20994..21803)
                     /locus_tag="PWS82_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0906212.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="PWS82_001066"
     CDS             complement(21790..22680)
                     /locus_tag="PWS82_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0906213.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="PWS82_001067"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="PWS82_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="PWS82_001068"
     CDS             complement(23348..24289)
                     /locus_tag="PWS82_001068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL0906214.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWS82_001069"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWS82_001069"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0906215.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="PWS82_001070"
     CDS             complement(25274..26470)
                     /locus_tag="PWS82_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0906216.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="PWS82_001071"
     CDS             complement(26488..27216)
                     /locus_tag="PWS82_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0906217.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         8004 a         5890 c         4780 g         8886 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtagac
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac
//
LOCUS       DANJJW010000026        26465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000026 DANJJW010000000
VERSION     DANJJW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            126..1679
                     /locus_tag="PWS82_001072"
     CDS             126..1679
                     /locus_tag="PWS82_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0906218.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2060..3844)
                     /locus_tag="PWS82_001073"
     CDS             complement(2060..3844)
                     /locus_tag="PWS82_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906219.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3859..5580)
                     /locus_tag="PWS82_001074"
     CDS             complement(3859..5580)
                     /locus_tag="PWS82_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906220.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5680..6441
                     /locus_tag="PWS82_001075"
     CDS             5680..6441
                     /locus_tag="PWS82_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0906221.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            7408..7676
                     /locus_tag="PWS82_001076"
                     /pseudo
     CDS             7408..7676
                     /locus_tag="PWS82_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            8348..11749
                     /locus_tag="PWS82_001077"
     CDS             8348..11749
                     /locus_tag="PWS82_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0906222.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(12173..12343)
                     /locus_tag="PWS82_001078"
     CDS             complement(12173..12343)
                     /locus_tag="PWS82_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0906223.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12537..12716)
                     /locus_tag="PWS82_001079"
     CDS             complement(12537..12716)
                     /locus_tag="PWS82_001079"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906224.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            13103..14215
                     /locus_tag="PWS82_001080"
     CDS             13103..14215
                     /locus_tag="PWS82_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0906225.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(14293..14586)
                     /locus_tag="PWS82_001081"
     CDS             complement(14293..14586)
                     /locus_tag="PWS82_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL0906226.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            14754..15068
                     /locus_tag="PWS82_001082"
     CDS             14754..15068
                     /locus_tag="PWS82_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL0906227.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            15089..17068
                     /locus_tag="PWS82_001083"
     CDS             15089..17068
                     /locus_tag="PWS82_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0906228.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            17081..17407
                     /locus_tag="PWS82_001084"
     CDS             17081..17407
                     /locus_tag="PWS82_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL0906229.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            17423..17941
                     /locus_tag="PWS82_001085"
     CDS             17423..17941
                     /locus_tag="PWS82_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906230.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            17941..19293
                     /gene="celB"
                     /locus_tag="PWS82_001086"
     CDS             17941..19293
                     /gene="celB"
                     /locus_tag="PWS82_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0906231.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(19377..20882)
                     /locus_tag="PWS82_001087"
     CDS             complement(19377..20882)
                     /locus_tag="PWS82_001087"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL0906232.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(20974..21564)
                     /locus_tag="PWS82_001088"
     CDS             complement(20974..21564)
                     /locus_tag="PWS82_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0906233.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            21743..23389
                     /locus_tag="PWS82_001089"
     CDS             21743..23389
                     /locus_tag="PWS82_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0906234.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            23438..24157
                     /locus_tag="PWS82_001090"
     CDS             23438..24157
                     /locus_tag="PWS82_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0906235.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            24636..25583
                     /locus_tag="PWS82_001091"
     CDS             24636..25583
                     /locus_tag="PWS82_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906236.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            25595..>26465
                     /locus_tag="PWS82_001092"
     CDS             25595..>26465
                     /locus_tag="PWS82_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906237.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPF"
BASE COUNT         8356 a         4534 c         5399 g         8176 t
ORIGIN      
        1 tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa attttttatt
       61 agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga gaaaaggaga
      121 gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa aaaattatcg
      181 agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta gtatttttct
      241 ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt gcagtagtcc
      301 gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg ggagttggcg
      361 gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt taccatcaat
      421 tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta cataataaac
      481 caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt caagcttttc
      541 ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta cctatctact
      601 taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta ctagaaaacg
      661 aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact aagatgagta
      721 cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat gggagataca
      781 ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg aactcaacta
      841 acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag caaaaggtaa
      901 atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac tctctctata
      961 tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa tggttgcgtt
     1021 ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg gacaacacat
     1081 atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat gtacgtctgc
     1141 tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa tcagttcagc
     1201 cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc tgtacatcag
     1261 ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc ggaatcactt
     1321 ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc ttcgttacaa
     1381 cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac ggaggacgct
     1441 cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg atagatcttt
     1501 ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca aaattttatt
     1561 tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc cgttcaactg
     1621 ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa ggagattaaa
     1681 gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat tcaaaaaaca
     1741 gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga gcaacaagaa
     1801 gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc cgataggttg
     1861 tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt cactacttac
     1921 cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt gtgtttctag
     1981 tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac atgatcgata
     2041 gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga ctggcataaa
     2101 gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg ccattcttca
     2161 ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc acaaaacttg
     2221 tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt cgtgtatcta
     2281 cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg actcgagcaa
     2341 ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa atgatcgtat
     2401 catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca gccgcttcga
     2461 taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct accgttcctt
     2521 caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct ccacgtgaaa
     2581 gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca taaaaacgca
     2641 tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc gctaccgttt
     2701 gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat gtgtaaccaa
     2761 actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt gcatctggaa
     2821 ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt gctgccattg
     2881 cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa atctgattga
     2941 tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga acaagcatag
     3001 caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg gccggataga
     3061 tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta tttacttcgt
     3121 gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga ttaccagcta
     3181 agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt tcagaaatct
     3241 gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc agcaataaga
     3301 cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca gctattgtta
     3361 cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt tgattgagac
     3421 cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac gacatcggta
     3481 atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt tcgcttaacg
     3541 ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa cagccgatca
     3601 gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc tgtccaatac
     3661 ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc aagggcatgg
     3721 ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa tatttatgtt
     3781 caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt tttttctttt
     3841 ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat aagtttggca
     3901 attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat ctaaaacaat
     3961 gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga gagttttctc
     4021 cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt ctaaagcgga
     4081 gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag caatacatat
     4141 ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc tgtccaatcc
     4201 gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt tagacaactc
     4261 ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc cgctgaataa
     4321 gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag tcatttgagt
     4381 aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc tcaagagaag
     4441 tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg tttttccttt
     4501 aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat atttaaatgt
     4561 gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta ctgttttgat
     4621 cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc gtaatgaggc
     4681 aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag caagtgtcag
     4741 catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt ctgctacaag
     4801 atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa aagcaattgg
     4861 agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt cagcaaagct
     4921 cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca tattaatccc
     4981 agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga tactcaaaga
     5041 taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa caacagagat
     5101 caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg tgaaaacgca
     5161 aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct ggacattatc
     5221 gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga tatccttcat
     5281 tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa acctagcgga
     5341 agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa acgctacaag
     5401 cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta tacctgtgtc
     5461 gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta aacagatcat
     5521 cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta agatagacat
     5581 attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa cttgaaggtc
     5641 aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat gtatacgatt
     5701 ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg ggatgaacaa
     5761 ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga tatcgatgat
     5821 atttttatgc tttatgatat cgttttttat agaaaattag atataccgat caaacagatg
     5881 aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga aacagaaaga
     5941 agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag ggagcaaaaa
     6001 aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga agagatccct
     6061 tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa atttctacct
     6121 aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac gatgtatgga
     6181 ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg ggagaaaaaa
     6241 gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc ggaacgtaat
     6301 aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg agaagtgatc
     6361 ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata ttatcatgca
     6421 tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat gatggttgtt
     6481 ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg attattagtc
     6541 gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg gaatggttgc
     6601 aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa aaataattgt
     6661 attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa attcatattg
     6721 ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata aaagcattac
     6781 acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa aataaataga
     6841 tggattgaat aaacatattt tttaatcaaa tagtccctac tgtgactttc ctcagctatc
     6901 ggtgatacta ccgatagcat ttttttgctt ataaaataat tataagtatt tagagatttt
     6961 tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta ttgttatttt tatatattct
     7021 ttttaagaat gattgttttt ttatagaaat tttaaaaaat aaaacacgtt attgatggat
     7081 agttagtttg ttatttggta atattataga agttgactgg tagtgattta tttttcgtcg
     7141 ttttataaaa gtgttatttt ttggaaaaat tcacaaatat gtaacgaatg tttttctgga
     7201 atttgctgtg ttaaaaagca gtatgactaa tataaatact attgtcattt atgcaatttt
     7261 ttatggatag taattttaga ggggaatcaa tgaaggaaga atagaaggga aaaatagttg
     7321 caaatacgcc agttagcaaa aatagcaata catcttcaag ctttaaaatt cgagagaatc
     7381 atcttggtag tggaatcaac tacaacaaaa ggattaaacc aattaataga tagaattacc
     7441 aatggaaaaa tagaaaaggt agttattcta tacaaaagtt gtttaattcg cttcggttat
     7501 gaacttagtg aaaccctttg caatatatgg tacagaatat agaaattatt gacattacag
     7561 aaaatgatga agatttaata cagattgtca ctatttgtag atacctttta caaggaaacc
     7621 gagccaataa agctaagaag atgattaagg agttgatgga agacgataca agttaagaaa
     7681 gtgaaactac gcccaaataa agagcaaaaa gagtaattgg acgtggaaaa tgcaagaaat
     7741 gaaccatcga ttcggtggac agtttattag taatgacggt ttgagaacat ccatgacgag
     7801 aatgaaggaa catcctaaat gcgtttagtt gaaccaagtg ccaaataata tggccaaata
     7861 agcgatcagt tatcgatgaa tcccaagtat tctaaactat atattcattt tgatgctaag
     7921 cattgatata tttttttggg attgatgaag gttaaccaat acagggattc actgatgaaa
     7981 ttctagagtc tgaccttggt atcaacgatt tagtgattgt ttctaatgga aaagtataaa
     8041 agtatataag tacattaaca aaataactat tagggttacg ctccagcttg tgcaaggcac
     8101 tggcaggagg aatcgtgaaa tcataattat ggttaagcca cacggacaac taaaagtcat
     8161 acaaagctat cgaatcaatc aacagaagac aatcggcaat cattagcttt gtttacagct
     8221 tacattgcaa cgcttgagtt gaaccgaaaa tacgagggag gtaacgcagc cattttgagt
     8281 tggggaatct ttttagaaaa ttatcgggta ccttgttgtt tagtcaaaat tataaggggg
     8341 agtaatagtg aggaacaaat ttcataaact gttaagtatg tttctagtta tgcttattgt
     8401 gatcaatcaa tttgggaaca taacggttct agctgagtct ttagcagaac agccagagcg
     8461 ttcgctgacg atcgaagttg atcaagagga ggctgctttg ggtgagcagt tccagttgcg
     8521 agtcagagga acaccagacc tgctaaatgc attggtcatt gataagagtg aggatttgat
     8581 tgaaggggaa gtgaagcaac tcaatgatta cgaacgtctg attacttatt cttcccagca
     8641 ttcaggtaaa tacgaattga ccgcaagaag cggtgatatt caatcaaata cggtctcaat
     8701 agaaatcagt gtaccagaag aaagcacaag tgagtctttt aatgtaccag aagaaagcac
     8761 aagtgaatct tttaatatac cagaagaaag cttaagtgaa tcttttaatg aagccagatc
     8821 atctgagcca tctataacgc aggaatctca gattgaaaga caagaggagc tcttgaatta
     8881 tgacatcggc atctctgctg agcgccccac tttgccgagc aggcatacac aaacgttaaa
     8941 gggatacatt caaggaggcg ctggcgatat tcttgaagaa tatggggaga tggtcgtacg
     9001 agttcctaga gcatcgattg gcttaattga gttcattaga gtggaggtcg ctgatgattc
     9061 acctttaaag ccactggcat tttaccaaga aggcacagac gttgtttttc catttacctt
     9121 tgattatgaa ggattaaatg aagccgctca gtcgtataca ttcagtatca cgtttgaagt
     9181 tgctttcttt caatcaggcg gtactgagcc aaataatgac tttcaagtga gtcttactta
     9241 tgctgagcga gcagcaaaca cggaattttc aattacgcct attccttcat caagacccat
     9301 tttttcgaaa ttatactatg gctctagaga aatcgacagc caaggaaatc ttgggaatct
     9361 gctgattgac gggcaagacg tgaccaacag taataaattt attttgcagg taaattatga
     9421 aggatcacga aaagacggtg tagtggttac cgatgtacta ccgcaaggaa caactttggc
     9481 accgaatccg actacatgga gttccactgg aaatagagaa cctatttcag gtggggttcg
     9541 gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga actcattatg tttccgagga
     9601 atttgtggac aaagtatctt ttaataagga aacgaataca cttgttgtgg actttggtaa
     9661 tctagcagag ggtcaatcgt attttattga atatgctctt gaagtgaccg atgtcgactt
     9721 gttcaatgaa ggaagacaag aggatgggtt ttctcggaat cgagcgagta tgacctacaa
     9781 taatcaggta atgagccgag actaccgctt gagaatccct gaaagtaatc ttccggcaat
     9841 ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc aatgaaaatg aactaacgta
     9901 tactctgcat gtctcgatgt tttctggaac aagaaatacg cttgttattg aagatgtttt
     9961 ggaagaaggc atggaatttg tgtcatttct atcagatatt ccctctttca ttgatgccga
    10021 agtcaataat ggaatagttc gtttcagtgc gaataatacg attggtggtc cgacaagttt
    10081 taccctctct tttaaggtca atgtagaggc gtatgcggtc ggcgatcaaa ttttaaactt
    10141 tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt actgtaagaa caaggaaaat
    10201 cgatgggcgt attaaagtaa ttaaaactga tgagaatggc caaaggctgg caggagcaaa
    10261 atttgaaatt ttatcaagcg attcacaagt gatacaagaa ggtataacgg cagaaaacgg
    10321 agaattttta agtgaaccgc ttcctgtagg tagctatcaa gtccgagaaa cagaagcacc
    10381 ggaagggtat cttttagatg cgacggtaca tgatgttttt ataaatgaat actccataga
    10441 agaagtgacg ttaaccattg aaaacatcct tgatacgggt ggtgttgaac taattaaaca
    10501 tgctgaaggc agtggtgaag tccttcaagg agctgttttt gagttacaga accgagaagg
    10561 agaaacgcta caaaccggtt taactacagg agaagatgga aaactggcaa ttgacggttt
    10621 agcaccagga gcatatcagc tggtagaaac tcaagcgcca atagggtatg aattagatgc
    10681 gacaccaatc gagtttgaga tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga
    10741 aaaccgactt acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaaat
    10801 cctgcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggtct
    10861 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
    10921 ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg agtttgagat
    10981 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta caccaggcgg
    11041 tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag ctgtttttga
    11101 gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag cagatggaaa
    11161 actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc aagcgccaac
    11221 ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc agacagcggt
    11281 ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga caaagatcga
    11341 tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
    11401 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
    11461 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
    11521 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
    11581 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
    11641 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
    11701 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
    11761 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
    11821 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
    11881 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
    11941 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
    12001 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
    12061 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
    12121 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
    12181 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
    12241 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
    12301 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
    12361 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
    12421 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
    12481 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
    12541 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
    12601 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
    12661 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
    12721 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
    12781 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
    12841 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
    12901 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
    12961 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
    13021 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
    13081 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
    13141 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
    13201 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
    13261 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
    13321 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
    13381 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
    13441 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
    13501 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
    13561 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
    13621 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
    13681 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
    13741 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
    13801 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
    13861 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
    13921 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
    13981 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
    14041 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
    14101 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
    14161 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
    14221 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
    14281 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
    14341 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
    14401 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
    14461 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
    14521 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
    14581 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
    14641 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
    14701 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
    14761 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
    14821 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
    14881 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
    14941 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
    15001 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
    15061 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
    15121 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
    15181 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
    15241 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
    15301 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
    15361 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
    15421 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
    15481 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
    15541 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
    15601 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
    15661 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
    15721 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
    15781 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
    15841 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
    15901 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
    15961 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
    16021 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
    16081 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
    16141 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
    16201 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
    16261 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
    16321 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
    16381 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
    16441 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
    16501 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
    16561 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
    16621 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
    16681 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
    16741 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
    16801 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
    16861 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
    16921 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
    16981 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
    17041 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
    17101 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
    17161 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
    17221 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
    17281 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
    17341 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
    17401 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
    17461 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
    17521 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
    17581 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
    17641 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
    17701 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
    17761 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
    17821 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
    17881 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
    17941 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
    18001 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
    18061 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
    18121 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
    18181 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
    18241 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
    18301 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
    18361 acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt aggaacttca
    18421 ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc
    18481 aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc tcgttctttc
    18541 actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa tggattatta
    18601 gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt tgttacgcag
    18661 ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc attgtggatc
    18721 gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt attagctaat
    18781 atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa caattcttat
    18841 gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc attcctcgcg
    18901 aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt attcaatatc
    18961 aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc ggttccgttt
    19021 ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct ggaaatcgtc
    19081 aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc atttgttggt
    19141 agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt
    19201 atctggttcc catttatcaa attctatgat aataaattat taaatgaaga aagagaaaag
    19261 gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa
    19321 aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa ataccattat
    19381 tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc tacaccattg
    19441 attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg cggagcgctt
    19501 tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac gatcaaatct
    19561 ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc aaatgctggg
    19621 tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc aaaatcatgt
    19681 cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg gccatctgga
    19741 tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg tgcatagtcc
    19801 acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg tacacgagct
    19861 tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc ggcaatgatc
    19921 gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata agcagtatcg
    19981 cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc tttatctgaa
    20041 cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg aatctggaat
    20101 agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa aacaactgtt
    20161 tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg
    20221 atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa agtcaagccg
    20281 tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg agtcatacca
    20341 ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt tgtcaaatgg
    20401 gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa tgctgttttt
    20461 ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt tttacctttt
    20521 gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa agccatagca
    20581 agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga attttcgtat
    20641 ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa atattcttct
    20701 ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct tgaatcataa
    20761 cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct tgttgaaaca
    20821 aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa agagaagctc
    20881 attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact ctttttaata
    20941 tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg acacagggga
    21001 caagcggatg agtattttca taaacaatga actgactgta atagatagga gaacgaatag
    21061 tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat tggtaagtaa
    21121 aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat
    21181 accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact
    21241 tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa caatctctaa
    21301 aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt ttcctaatag
    21361 gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg agcgttggat
    21421 agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag ctatatcgtg
    21481 agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa atataacggg
    21541 tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat aaaaggctgt
    21601 atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa
    21661 aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata gtaatgtttt
    21721 gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag atcaattaac
    21781 accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata tcatttcaag
    21841 attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa atgctaaggc
    21901 tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac tgatctctac
    21961 agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat tgaatgaata
    22021 tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt taggcctgtt
    22081 gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg gtggcggaga
    22141 agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc cttgtatgag
    22201 tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat
    22261 tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca tcatgaaagt
    22321 catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat ttatcggtat
    22381 gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt atacatcggc
    22441 gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt tgttagatac
    22501 cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct tcaaattagt
    22561 cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca atgcacagtt
    22621 actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg tagttattgc
    22681 attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg ttacgttgaa
    22741 agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt cattttttat
    22801 gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat tattttatac
    22861 accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac tcttcctagg
    22921 actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg cggcaattaa
    22981 ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca ttcgtttatt
    23041 tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt gtaccttgat
    23101 cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc gcaggacatt
    23161 gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa agatgatttt
    23221 tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc ttgtagtagc
    23281 aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg cagaaagact
    23341 actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag tgaatcagaa
    23401 gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata agtttttagc
    23461 agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag gaacgatcga
    23521 agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg aagatgcggt
    23581 tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt tgcataaacc
    23641 tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg atttattgga
    23701 agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag acacagaggg
    23761 cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac ctaaaaaaca
    23821 tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag atatccgttt
    23881 atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa aactccaaat
    23941 tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc atgaaggaaa
    24001 gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag
    24061 aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat atcgacccat
    24121 gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc taaaagaata
    24181 agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt tcggatggct
    24241 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttgttcgga
    24301 ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga ctcagagtta
    24361 gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca caacctctga
    24421 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    24481 actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca caacctctcc
    24541 tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg aaagcgctat
    24601 aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc
    24661 atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta ccgggtatgc
    24721 tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt
    24781 ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg gtgggttggg
    24841 aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa aatacaattt
    24901 tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt gcaatcatta
    24961 tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc ttactacctt
    25021 attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt cctgacaaag
    25081 gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg tataatgaac
    25141 cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt ggttataaca
    25201 gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa gctgctatgg
    25261 ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa cttattccac
    25321 taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat tttggcctgt
    25381 tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt ttggatacgt
    25441 atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct gcaggacttt
    25501 accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg
    25561 atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt
    25621 ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga ttgcactctt
    25681 tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta ccgatgagtc
    25741 catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat ttggttatac
    25801 gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca ctgtacttgt
    25861 tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt atgcgatttc
    25921 acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta cgatgttgtt
    25981 ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt taaaagatac
    26041 gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg tgatgcgcac
    26101 attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg atggagctag
    26161 cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta ttgcgacaat
    26221 cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac tatacatcca
    26281 agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta atattcaata
    26341 tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct cagttccagg
    26401 agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga tcagctatcc
    26461 atttt
//
LOCUS       DANJJW010000027        25032 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000027 DANJJW010000000
VERSION     DANJJW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWS82_001093"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWS82_001093"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL0906238.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWS82_001094"
     CDS             complement(1172..1867)
                     /locus_tag="PWS82_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL0906239.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWS82_001095"
     CDS             complement(1880..2596)
                     /locus_tag="PWS82_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL0906240.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWS82_001096"
     CDS             2926..3534
                     /locus_tag="PWS82_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL0906241.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="PWS82_001097"
     CDS             3859..4182
                     /locus_tag="PWS82_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL0906242.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="PWS82_001098"
     CDS             4364..5734
                     /locus_tag="PWS82_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL0906243.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="PWS82_001099"
     CDS             complement(5864..7165)
                     /locus_tag="PWS82_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0906244.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="PWS82_001100"
     CDS             complement(7519..9477)
                     /locus_tag="PWS82_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL0906245.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="PWS82_001101"
     CDS             complement(9654..9920)
                     /locus_tag="PWS82_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0906246.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWS82_001102"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWS82_001102"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL0906247.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWS82_001103"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWS82_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL0906248.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="PWS82_001104"
     CDS             13264..13827
                     /locus_tag="PWS82_001104"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0906249.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="PWS82_001105"
     CDS             complement(14080..14601)
                     /locus_tag="PWS82_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL0906250.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="PWS82_001106"
     CDS             complement(14604..15446)
                     /locus_tag="PWS82_001106"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL0906251.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="PWS82_001107"
     CDS             complement(15439..16164)
                     /locus_tag="PWS82_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL0906252.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="PWS82_001108"
     CDS             16340..16636
                     /locus_tag="PWS82_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906253.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="PWS82_001109"
     CDS             17204..18796
                     /locus_tag="PWS82_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0906254.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="PWS82_001110"
     CDS             18809..19549
                     /locus_tag="PWS82_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0906255.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="PWS82_001111"
     CDS             19717..21066
                     /locus_tag="PWS82_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0906256.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="PWS82_001112"
     CDS             complement(21194..21784)
                     /locus_tag="PWS82_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0906257.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="PWS82_001113"
     CDS             22020..23099
                     /locus_tag="PWS82_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0906258.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23320..24672
                     /gene="brnQ"
                     /locus_tag="PWS82_001114"
     CDS             23320..24672
                     /gene="brnQ"
                     /locus_tag="PWS82_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0906259.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7150 a         5130 c         4881 g         7871 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    23281 aaatttacga cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt
    23341 aaagactatc tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt
    23401 atctttcccg ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt
    23461 ttcttggtta ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa
    23521 agctcaggtg tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc
    23581 atcttgcttt acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct
    23641 ttcgagattg gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt
    23701 ttcagtattg tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta
    23761 gattatgttg gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc
    23821 cttgcttttt tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa
    23881 gagcatgctt ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct
    23941 ttagcatttg ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct
    24001 gaaatcgcta aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt
    24061 tatactttgc tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat
    24121 ggcgggatca cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta
    24181 ttagcattta tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt
    24241 gcagaaacat tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct
    24301 agtatccttc cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg
    24361 cctgttttga tgttcatcta tccattagca atgacactga ttttactagt gttagttggt
    24421 cctttgttta agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct
    24481 tcgatttttg atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa
    24541 aatcttttat atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc
    24601 gtaccagcag ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa
    24661 gtagcagatt gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa
    24721 ttttgaaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactgact
    24781 tttgaacacc gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat
    24841 attttgtgaa aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg
    24901 cttttgaaca ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg
    24961 gcaaagtagc tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat
    25021 caaacaacat tt
//
LOCUS       DANJJW010000028        24424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000028 DANJJW010000000
VERSION     DANJJW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..839)
                     /locus_tag="PWS82_001115"
     CDS             complement(<1..839)
                     /locus_tag="PWS82_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0906260.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1063..3003)
                     /gene="thrS"
                     /locus_tag="PWS82_001116"
     CDS             complement(1063..3003)
                     /gene="thrS"
                     /locus_tag="PWS82_001116"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL0906261.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3340..3519)
                     /locus_tag="PWS82_001117"
     CDS             complement(3340..3519)
                     /locus_tag="PWS82_001117"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL0906262.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3566..5389)
                     /gene="cadA"
                     /locus_tag="PWS82_001118"
     CDS             complement(3566..5389)
                     /gene="cadA"
                     /locus_tag="PWS82_001118"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0906263.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5667..7946
                     /locus_tag="PWS82_001119"
     CDS             5667..7946
                     /locus_tag="PWS82_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0906264.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8039..8686)
                     /locus_tag="PWS82_001120"
     CDS             complement(8039..8686)
                     /locus_tag="PWS82_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906265.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8865..9584)
                     /locus_tag="PWS82_001121"
     CDS             complement(8865..9584)
                     /locus_tag="PWS82_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906266.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9715..10866)
                     /locus_tag="PWS82_001122"
     CDS             complement(9715..10866)
                     /locus_tag="PWS82_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL0906267.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11083..11823)
                     /locus_tag="PWS82_001123"
     CDS             complement(11083..11823)
                     /locus_tag="PWS82_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0906268.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11827..12150)
                     /gene="rsfS"
                     /locus_tag="PWS82_001124"
     CDS             complement(11827..12150)
                     /gene="rsfS"
                     /locus_tag="PWS82_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL0906269.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12207..12800)
                     /gene="yqeK"
                     /locus_tag="PWS82_001125"
     CDS             complement(12207..12800)
                     /gene="yqeK"
                     /locus_tag="PWS82_001125"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL0906270.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12784..13434)
                     /locus_tag="PWS82_001126"
     CDS             complement(12784..13434)
                     /locus_tag="PWS82_001126"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL0906271.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13444..13755)
                     /gene="yhbY"
                     /locus_tag="PWS82_001127"
     CDS             complement(13444..13755)
                     /gene="yhbY"
                     /locus_tag="PWS82_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL0906272.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13771..14880)
                     /gene="yqeH"
                     /locus_tag="PWS82_001128"
     CDS             complement(13771..14880)
                     /gene="yqeH"
                     /locus_tag="PWS82_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL0906273.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14877..15404)
                     /locus_tag="PWS82_001129"
     CDS             complement(14877..15404)
                     /locus_tag="PWS82_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL0906274.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15584..16369)
                     /locus_tag="PWS82_001130"
     CDS             complement(15584..16369)
                     /locus_tag="PWS82_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL0906275.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16362..17231)
                     /locus_tag="PWS82_001131"
     CDS             complement(16362..17231)
                     /locus_tag="PWS82_001131"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL0906276.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17232..18611)
                     /locus_tag="PWS82_001132"
     CDS             complement(17232..18611)
                     /locus_tag="PWS82_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL0906277.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18619..19038)
                     /gene="fabZ"
                     /locus_tag="PWS82_001133"
     CDS             complement(18619..19038)
                     /gene="fabZ"
                     /locus_tag="PWS82_001133"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0906278.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19063..19539)
                     /gene="accB"
                     /locus_tag="PWS82_001134"
     CDS             complement(19063..19539)
                     /gene="accB"
                     /locus_tag="PWS82_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL0906279.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19542..20777)
                     /gene="fabF"
                     /locus_tag="PWS82_001135"
     CDS             complement(19542..20777)
                     /gene="fabF"
                     /locus_tag="PWS82_001135"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL0906280.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20796..21533)
                     /gene="fabG"
                     /locus_tag="PWS82_001136"
     CDS             complement(20796..21533)
                     /gene="fabG"
                     /locus_tag="PWS82_001136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL0906281.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21545..22462)
                     /gene="fabD"
                     /locus_tag="PWS82_001137"
     CDS             complement(21545..22462)
                     /gene="fabD"
                     /locus_tag="PWS82_001137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0906282.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22569..22793)
                     /locus_tag="PWS82_001138"
     CDS             complement(22569..22793)
                     /locus_tag="PWS82_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0906283.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22846..23808)
                     /locus_tag="PWS82_001139"
     CDS             complement(22846..23808)
                     /locus_tag="PWS82_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL0906284.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23818..24258)
                     /locus_tag="PWS82_001140"
     CDS             complement(23818..24258)
                     /locus_tag="PWS82_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0906285.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6682 a         5438 c         4248 g         8056 t
ORIGIN      
        1 tccataaaat attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg
       61 tttttagcca tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa
      121 ttagttgccg cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg
      181 gctatcttgc taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg
      241 cttttcgcca gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc
      301 caaagcactt tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt
      361 tcaaaaatga ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa
      421 gaagaggcgc caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact
      481 ttttggatcg tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg
      541 ttaccaagtt tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct
      601 tcatcgattc cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga
      661 aaaccgattt ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct
      721 aattcattag ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac
      781 tttttttctg ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata
      841 agttcctcca cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac
      901 ggtcgtatac tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc
      961 aatcccacag attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg
     1021 gattgctttc tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta
     1081 atttttgact tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc
     1141 tttacttcca taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac
     1201 taattggtaa gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt
     1261 acgatcatca acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc
     1321 ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca
     1381 tgttggaaaa gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga
     1441 aacaattccg cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata
     1501 tgtcaaatca aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc
     1561 tgtaccaaga gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc
     1621 ttctgcttca aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc
     1681 tttttcccac attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct
     1741 caagcggaaa cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag
     1801 cgtacgttta aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa
     1861 cgtcatttct cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat
     1921 cattcctaat tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta
     1981 gaccatcatg tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat
     2041 atccattggc gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa
     2101 ctcaacatct cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc
     2161 tacgatataa cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa
     2221 accagaacct acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg
     2281 gtctctttct tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa
     2341 aaaggctgtc ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc
     2401 acctgccact gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc
     2461 acgacacaaa tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa
     2521 ttcactaatc agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt
     2581 tgaaagaaca cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc
     2641 gattgccggc aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa
     2701 ccctgaatcg atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc
     2761 ttgggccatc aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg
     2821 tgtgatgatc tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc
     2881 gttcacttta ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc
     2941 caatgttgtt gttccagact caaattcttt gactgcacca tctggaaaag taatttttat
     3001 cataatagtt tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa
     3061 aaatcctagc atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg
     3121 ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag
     3181 ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct
     3241 ctcttatcga agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt
     3301 aaccactaat aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt
     3361 tacaccgtat gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc
     3421 ctgcgtgttt ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg
     3481 actgcgacct tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga
     3541 tcgttctttt ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa
     3601 aaatggctgg atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt
     3661 cttgcaaaac agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa
     3721 aaatacaaat aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg
     3781 aaatcactac tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac
     3841 tttcactagc agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa
     3901 gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga
     3961 tactttctaa aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt
     4021 ggactttttc tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat
     4081 ttttcacatc aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat
     4141 ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg
     4201 acgtttctat ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat
     4261 tgactactgc ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt
     4321 ggctgttttt cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg
     4381 cactagctgc atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct
     4441 ctacttccaa tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac
     4501 gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc
     4561 tcattcctgc aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta
     4621 ccagtacctc agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca
     4681 aataagcaat caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag
     4741 gcttttcttt tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac
     4801 gtttttcagc gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat
     4861 ctccaggttt cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag
     4921 attctccttt ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact
     4981 ggtcatcaat atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat
     5041 gggcaattct tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg
     5101 cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt
     5161 attctttgac ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc
     5221 ttcctgaacg taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag
     5281 taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag
     5341 ctatcccacc aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc
     5401 ctcttttatg tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag
     5461 catagtcctt gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta
     5521 tcagcgtttc caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt
     5581 cgattcatca gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta
     5641 tttacggcgg aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg
     5701 atgaaacgat cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat
     5761 tgactcagga aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt
     5821 ccaatgaagc tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt
     5881 tgaaatacca tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag
     5941 atagtcctta ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc
     6001 taggtatcgc ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac
     6061 caattgccaa tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag
     6121 atagaataga tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt
     6181 tatctatggt agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg
     6241 aatcatcaag caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa
     6301 tcattcggga cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga
     6361 catccgcttt gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg
     6421 ctgaaaacgt tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat
     6481 tgccttcatt aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc
     6541 tgctgccaga gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg
     6601 aaaaagatcc gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct
     6661 atatccaagg catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta
     6721 cgatcctatc aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt
     6781 tacatcatag actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa
     6841 aagacgaaac acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat
     6901 acgcaacaga tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac
     6961 tggccaacca aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc
     7021 aatttgtcaa tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc
     7081 taacacgaca taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac
     7141 acttgcgtca aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc
     7201 gaacatatcc agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg
     7261 atttcgcacc atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt
     7321 tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag
     7381 accttttcgt ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa
     7441 aagaaatcac tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag
     7501 caagaaaagg agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt
     7561 ggttagcaga taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca
     7621 tagctaaaac acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac
     7681 aacaagtcca gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc
     7741 cagccttttt agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag
     7801 aacatttttc ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc
     7861 atgaattgac cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt
     7921 catcttatga gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag
     7981 ttgtcttttt atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt
     8041 attctatata taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca
     8101 ttttcagttc ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa
     8161 tcccgccatt gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt
     8221 ggaaattgct gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt
     8281 atgcactatc gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa
     8341 ttttttcagg acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat
     8401 actcttcttc aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat
     8461 aattttgtct ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca
     8521 gataccctgt accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt
     8581 tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac
     8641 cgcttaagat acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc
     8701 atcttccaca tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt
     8761 gaattagaaa agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct
     8821 tgcttttcga aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc
     8881 acattatgga acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca
     8941 aatgtttctt gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca
     9001 gcgattgaaa aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc
     9061 gctacttcac cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg
     9121 tctttttcca ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt
     9181 gctgtattgt caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc
     9241 ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca
     9301 atcaccattg agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct
     9361 cctgcacctt ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt
     9421 ttagcgcgat caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat
     9481 tttgctgccg cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct
     9541 ttagcagttt tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt
     9601 ctgatttgat agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat
     9661 tctgtgaagc tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac
     9721 tcggattggg atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg
     9781 atcacttcta actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat
     9841 agaaatctct tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg
     9901 aagataattt ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata
     9961 acaagataat cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa
    10021 atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt
    10081 catctgatag atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata
    10141 cggccaataa gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg
    10201 cggcacaagt ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact
    10261 ggctatcctc tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg
    10321 atgtaattcc atagggtgga catacttggc attttccttt gcataactta atcccaagat
    10381 atgatttggc gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt
    10441 cattttttgc ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct
    10501 atcttgattc tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc
    10561 tgttccaaaa cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc
    10621 aaaatagtca gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc
    10681 attttgtaaa gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg
    10741 caaaaaattc ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat
    10801 ttccacatga tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct
    10861 tttcatgcgc atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa
    10921 ggcaaaaatc ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct
    10981 gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt
    11041 gaagatttat tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac
    11101 aaagaaccag cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc
    11161 gactttcgaa aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg
    11221 ctcttgatgt agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa
    11281 aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa
    11341 agcaaaatta tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata
    11401 ggtggagtgt acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc
    11461 aaacacttgc tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt
    11521 aattgcctga tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat
    11581 cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc
    11641 taaccctgtc actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca
    11701 tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca
    11761 ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc
    11821 catatgttaa tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc
    11881 cagattatag aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc
    11941 gatcaatacc cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc
    12001 ttcttcttta tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga
    12061 acagatcatg aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc
    12121 ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat
    12181 gatgatttcc tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg
    12241 attgtttttg gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt
    12301 tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca
    12361 ggaaaatctc gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc
    12421 atcttagcag ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt
    12481 cctaattccc gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa
    12541 ttcaataact ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt
    12601 cgttcttttg cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg
    12661 tattttttgg caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt
    12721 tcgctcattc tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca
    12781 ttactatagt ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg
    12841 aacaaggtag cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat
    12901 gtcgatctca ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg
    12961 tcgacgtata cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg
    13021 caaatattct accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg
    13081 cgtcaattcc ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt
    13141 ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt
    13201 tgcatcaatc gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac
    13261 gcggtcaaga cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac
    13321 gggattgaat gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat
    13381 ttcttcagga aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac
    13441 tccttatatt gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt
    13501 gtataataca ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa
    13561 tacttgagct gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag
    13621 ttctcttttt tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt
    13681 tccgatttga aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt
    13741 tcctcttaaa ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc
    13801 tacacccttt ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag
    13861 accggcaaaa acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg
    13921 aaatgcctcg acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata
    13981 aaattcgtct gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc
    14041 aataaatgaa gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag
    14101 tgtttgccct tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat
    14161 ccgcaaatct tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt
    14221 atcaatcaaa aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg
    14281 aaactgcgaa gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat
    14341 aagcgtcgat tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta
    14401 ttcttcgatt ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa
    14461 tacatctact ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt
    14521 agtttttttc aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt
    14581 gtcaccgaca aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac
    14641 cacattgacg atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc
    14701 atccgtcaat gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca
    14761 atagacttcc ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc
    14821 accgggtttg tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat
    14881 tcgattccac ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt
    14941 ttttcccaaa aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca
    15001 aggatgctac gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct
    15061 accattacta tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt
    15121 gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg
    15181 acacgttttg aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct
    15241 aaaatccaag taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg
    15301 tctaaatcgg tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga
    15361 tagatggctt ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg
    15421 caaaaaatga aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa
    15481 aaaaaactac ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca
    15541 aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc
    15601 tcttgtatcg tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt
    15661 caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga
    15721 acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt
    15781 tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc
    15841 cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac
    15901 ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct
    15961 ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg
    16021 ctcctacacc gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact
    16081 tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa
    16141 aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg
    16201 gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat
    16261 caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc
    16321 gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat
    16381 cctttctgtg tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga
    16441 acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga
    16501 agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg
    16561 ggttctgcta gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc
    16621 gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca
    16681 gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa
    16741 gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc
    16801 ttttctccga caatcgttcc catacttccc atgataaaat taggatccat cactccaata
    16861 atgaagggaa tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt
    16921 ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt
    16981 acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc
    17041 catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct
    17101 ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat
    17161 ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta
    17221 aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct
    17281 tcttgtaaaa atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc
    17341 atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc
    17401 atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa
    17461 tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc
    17521 ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca
    17581 ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga
    17641 cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct
    17701 gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa
    17761 aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct
    17821 ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca
    17881 gaaggagatt cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct
    17941 aaatgaatga catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga
    18001 taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga
    18061 gctgaagaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg
    18121 ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat
    18181 gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct
    18241 tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga
    18301 ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa
    18361 aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc
    18421 tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct
    18481 aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct
    18541 agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact
    18601 ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt
    18661 accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc
    18721 aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa
    18781 tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc
    18841 tgcttgagcc attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg
    18901 tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc
    18961 aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt
    19021 tatttcttgt atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct
    19081 aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac
    19141 tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac
    19201 ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg
    19261 cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc
    19321 tttttctttc attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc
    19381 cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt
    19441 attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc
    19501 aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc
    19561 atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa
    19621 cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct
    19681 acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg
    19741 atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta
    19801 tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct
    19861 ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct
    19921 tccatcgcaa gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata
    19981 tggtaagcgt cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga
    20041 gcttgtgcat gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa
    20101 ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt
    20161 gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca
    20221 gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc
    20281 gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa
    20341 gcaatatttc cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt
    20401 tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc
    20461 ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc
    20521 atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg
    20581 ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta
    20641 atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt
    20701 aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta
    20761 attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca
    20821 tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt
    20881 gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg
    20941 actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta
    21001 catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca
    21061 gctttactag ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag
    21121 agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata
    21181 ttgaaggttc caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc
    21241 atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc
    21301 ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa
    21361 actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct
    21421 ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg
    21481 ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc
    21541 ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg
    21601 gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga
    21661 tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt
    21721 gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg
    21781 tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca
    21841 gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga
    21901 tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt
    21961 cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact
    22021 tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca
    22081 ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg
    22141 caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac
    22201 tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga taatacgcga
    22261 tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg
    22321 taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct
    22381 ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac
    22441 taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct
    22501 tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat
    22561 tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt
    22621 ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat
    22681 aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca
    22741 gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata
    22801 tattcctccg atattgtttg ctttttattt ttataattta acagtctaca actccatgag
    22861 aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc
    22921 taaggtgatc tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga
    22981 tgtattccca tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg
    23041 ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt
    23101 gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc
    23161 tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt
    23221 attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc
    23281 tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa
    23341 caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat
    23401 caccagtccc ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac
    23461 aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc
    23521 ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat
    23581 aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat
    23641 gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga
    23701 cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt
    23761 ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca
    23821 cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc
    23881 ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac
    23941 ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc
    24001 actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt
    24061 cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc
    24121 gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag
    24181 ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt
    24241 ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga
    24301 tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta
    24361 tatgagcttt ttttaagatt ttactcattt aaaacaactg ataaatcagc atttataatt
    24421 tctt
//
LOCUS       DANJJW010000029        24069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000029 DANJJW010000000
VERSION     DANJJW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            133..3660
                     /locus_tag="PWS82_001141"
     CDS             133..3660
                     /locus_tag="PWS82_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL0906286.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWS82_001142"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWS82_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL0906287.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWS82_001143"
     CDS             7448..7789
                     /locus_tag="PWS82_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0906288.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWS82_001144"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWS82_001144"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL0906289.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWS82_001145"
     CDS             9146..11566
                     /locus_tag="PWS82_001145"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL0906290.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWS82_001146"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWS82_001146"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL0906291.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PWS82_001147"
     CDS             13000..13737
                     /locus_tag="PWS82_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0906292.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PWS82_001148"
     CDS             13751..14566
                     /locus_tag="PWS82_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0906293.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PWS82_001149"
     CDS             14579..15226
                     /locus_tag="PWS82_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0906294.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PWS82_001150"
     CDS             15242..15898
                     /locus_tag="PWS82_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0906295.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PWS82_001151"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PWS82_001151"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL0906296.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PWS82_001152"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PWS82_001152"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL0906297.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PWS82_001153"
     CDS             18240..18758
                     /locus_tag="PWS82_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0906298.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PWS82_001154"
     CDS             18865..19365
                     /locus_tag="PWS82_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0906299.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="PWS82_001155"
     CDS             complement(19645..20565)
                     /locus_tag="PWS82_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL0906300.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="PWS82_001156"
     CDS             20730..21743
                     /locus_tag="PWS82_001156"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL0906301.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWS82_001157"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWS82_001157"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL0906302.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="PWS82_001158"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="PWS82_001158"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0906303.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..>24069)
                     /locus_tag="PWS82_001159"
     CDS             complement(23383..>24069)
                     /locus_tag="PWS82_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0906304.1"
                     /translation="NTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENE
                     DLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGYYNGLTFHRVIKDFMIQGGDPKG
                     DGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLK
                     DDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDK
                     INILQEAKQK"
BASE COUNT         7960 a         4200 c         5096 g         6813 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgtt
//
LOCUS       DANJJW010000030        23831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000030 DANJJW010000000
VERSION     DANJJW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(55..789)
                     /locus_tag="PWS82_001160"
     CDS             complement(55..789)
                     /locus_tag="PWS82_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0906305.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="PWS82_001161"
     CDS             1116..1526
                     /locus_tag="PWS82_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL0906306.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="PWS82_001162"
     CDS             1519..2220
                     /locus_tag="PWS82_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL0906307.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="PWS82_001163"
     CDS             2220..3806
                     /locus_tag="PWS82_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906308.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="PWS82_001164"
     CDS             3825..4046
                     /locus_tag="PWS82_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL0906309.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="PWS82_001165"
     CDS             4043..4804
                     /locus_tag="PWS82_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL0906310.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="PWS82_001166"
     CDS             5184..5693
                     /locus_tag="PWS82_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0906311.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="PWS82_001167"
     CDS             complement(5768..6307)
                     /locus_tag="PWS82_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL0906312.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="PWS82_001168"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="PWS82_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL0906313.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="PWS82_001169"
     CDS             7320..8171
                     /locus_tag="PWS82_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL0906314.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="PWS82_001170"
     CDS             8199..8834
                     /locus_tag="PWS82_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL0906315.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="PWS82_001171"
     CDS             8854..9627
                     /locus_tag="PWS82_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0906316.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="PWS82_001172"
     CDS             10035..10832
                     /locus_tag="PWS82_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL0906317.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="PWS82_001173"
     CDS             10810..11223
                     /locus_tag="PWS82_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0906318.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="PWS82_001174"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="PWS82_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0906319.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="PWS82_001175"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="PWS82_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL0906320.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="PWS82_001176"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="PWS82_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL0906321.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="PWS82_001177"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="PWS82_001177"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL0906322.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="PWS82_001178"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="PWS82_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL0906323.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="PWS82_001179"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="PWS82_001179"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL0906324.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="PWS82_001180"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="PWS82_001180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL0906325.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="PWS82_001181"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="PWS82_001181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL0906326.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="PWS82_001182"
     CDS             21907..22485
                     /locus_tag="PWS82_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0906327.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="PWS82_001183"
     CDS             22537..23019
                     /locus_tag="PWS82_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL0906328.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="PWS82_001184"
     CDS             23136..23510
                     /locus_tag="PWS82_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906329.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DANJJW010000031        23064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000031 DANJJW010000000
VERSION     DANJJW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(110..565)
                     /locus_tag="PWS82_001185"
     CDS             complement(110..565)
                     /locus_tag="PWS82_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906330.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="PWS82_001186"
     CDS             complement(719..1927)
                     /locus_tag="PWS82_001186"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL0906331.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="PWS82_001187"
     CDS             complement(2039..2371)
                     /locus_tag="PWS82_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0906332.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="PWS82_001188"
     CDS             2519..3394
                     /locus_tag="PWS82_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0906333.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="PWS82_001189"
     CDS             complement(3476..4057)
                     /locus_tag="PWS82_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL0906334.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="PWS82_001190"
     CDS             complement(4063..5322)
                     /locus_tag="PWS82_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0906335.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="PWS82_001191"
     CDS             complement(5324..6370)
                     /locus_tag="PWS82_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906336.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="PWS82_001192"
     CDS             complement(6605..6880)
                     /locus_tag="PWS82_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL0906337.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWS82_001193"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWS82_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL0906338.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWS82_001194"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWS82_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL0906339.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="PWS82_001195"
     CDS             complement(9979..10659)
                     /locus_tag="PWS82_001195"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL0906340.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="PWS82_001196"
     CDS             complement(10708..11343)
                     /locus_tag="PWS82_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0906341.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="PWS82_001197"
     CDS             complement(11409..12812)
                     /locus_tag="PWS82_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL0906342.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="PWS82_001198"
     CDS             complement(12799..13830)
                     /locus_tag="PWS82_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906343.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="PWS82_001199"
     CDS             13861..14082
                     /locus_tag="PWS82_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL0906344.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="PWS82_001200"
     CDS             complement(14247..14984)
                     /locus_tag="PWS82_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL0906345.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="PWS82_001201"
     CDS             complement(15137..16477)
                     /locus_tag="PWS82_001201"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL0906346.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="PWS82_001202"
     CDS             complement(16714..18237)
                     /locus_tag="PWS82_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL0906347.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="PWS82_001203"
     CDS             complement(18615..19226)
                     /locus_tag="PWS82_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0906348.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="PWS82_001204"
     CDS             complement(19556..20272)
                     /locus_tag="PWS82_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0906349.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWS82_001205"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWS82_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL0906350.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="PWS82_001206"
     CDS             complement(20831..21628)
                     /locus_tag="PWS82_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL0906351.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="PWS82_001207"
     CDS             complement(21598..21969)
                     /locus_tag="PWS82_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0906352.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWS82_001208"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWS82_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL0906353.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DANJJW010000032        22911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000032 DANJJW010000000
VERSION     DANJJW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            115..927
                     /locus_tag="PWS82_001209"
     CDS             115..927
                     /locus_tag="PWS82_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL0906354.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            924..1724
                     /locus_tag="PWS82_001210"
     CDS             924..1724
                     /locus_tag="PWS82_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL0906355.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1885..3153
                     /gene="rseP"
                     /locus_tag="PWS82_001211"
     CDS             1885..3153
                     /gene="rseP"
                     /locus_tag="PWS82_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL0906356.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3221..4930
                     /locus_tag="PWS82_001212"
     CDS             3221..4930
                     /locus_tag="PWS82_001212"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL0906357.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5142..9500
                     /locus_tag="PWS82_001213"
     CDS             5142..9500
                     /locus_tag="PWS82_001213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL0906358.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9645..10118
                     /gene="rimP"
                     /locus_tag="PWS82_001214"
     CDS             9645..10118
                     /gene="rimP"
                     /locus_tag="PWS82_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL0906359.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10141..11316
                     /gene="nusA"
                     /locus_tag="PWS82_001215"
     CDS             10141..11316
                     /gene="nusA"
                     /locus_tag="PWS82_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL0906360.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11338..11631
                     /locus_tag="PWS82_001216"
     CDS             11338..11631
                     /locus_tag="PWS82_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL0906361.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11628..11939
                     /locus_tag="PWS82_001217"
     CDS             11628..11939
                     /locus_tag="PWS82_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL0906362.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11952..14258
                     /gene="infB"
                     /locus_tag="PWS82_001218"
     CDS             11952..14258
                     /gene="infB"
                     /locus_tag="PWS82_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL0906363.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14285..14632
                     /gene="rbfA"
                     /locus_tag="PWS82_001219"
     CDS             14285..14632
                     /gene="rbfA"
                     /locus_tag="PWS82_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL0906364.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14789..14962)
                     /locus_tag="PWS82_001220"
     CDS             complement(14789..14962)
                     /locus_tag="PWS82_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906365.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15410..17772
                     /locus_tag="PWS82_001221"
                     /pseudo
     CDS             15410..17772
                     /locus_tag="PWS82_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18140..19063
                     /gene="truB"
                     /locus_tag="PWS82_001222"
     CDS             18140..19063
                     /gene="truB"
                     /locus_tag="PWS82_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL0906366.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19067..20008
                     /gene="ribF"
                     /locus_tag="PWS82_001223"
     CDS             19067..20008
                     /gene="ribF"
                     /locus_tag="PWS82_001223"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL0906367.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20360..21145
                     /locus_tag="PWS82_001224"
     CDS             20360..21145
                     /locus_tag="PWS82_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0906368.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21158..22009
                     /locus_tag="PWS82_001225"
     CDS             21158..22009
                     /locus_tag="PWS82_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL0906369.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22133..22585)
                     /locus_tag="PWS82_001226"
     CDS             complement(22133..22585)
                     /locus_tag="PWS82_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906370.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7790 a         3986 c         4988 g         6147 t
ORIGIN      
        1 agaaaaaatt attcagtttt taaataaaaa aacaatgatt tcataggaaa aacagttaga
       61 tattgctata atacttaaga tagtgggaat cttttaagta taggaggaaa aagaatgtta
      121 cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa gcgaatttca atttaatacc
      181 gatggaccaa ttcctcagca cattgccatc atcatggacg gcaatggtcg ttgggcgcaa
      241 aaccgtcgtt tgccacgagt tgctggccat aaagaaggca tggaaacagt aaagaaagtc
      301 acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc tttatgcctt ttcgactgaa
      361 aactggaagc gaccaaaaga tgaagtaagt tttctcatgc aactacctgt cgacttcttt
      421 gatacgttcg taccagaatt gattaaagaa aatgtcaaag ttcatgtcat gggttatgaa
      481 aacgtattgc cagaacatac gcaagatgct gtaagacgtg caattgagca aacaaaaaac
      541 aatactggaa tggtattgaa ttttgctttg aattatggaa gcagagcaga gatcgtgact
      601 gctgtccaag aaattgccga agaagtagca aaaggagaaa tacacgcaga agaaatcgat
      661 gatgaattga ttgctaagca tttaatgaca ggtttcttgc ctaaagagtt acaagatcct
      721 gagctgatga tccgtacaag tggagaagag cgaattagta attttctgtt atggcagatc
      781 gcatatagtg agctgtattt tacaaaagcg ctttggcctg attttgatgg tgcgcatctt
      841 gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat tcggcggtgt aaaaaaaaca
      901 gaagatgaga aaggggatca gtcatgaaac aacgtgtggt cacagcagcc gtggcgctag
      961 tcttgtttat tccgattata tggatcggag gcatagcggt tgagcttaca gcagcaatct
     1021 tagcagtaat aggagtttac gagctgtttc ggatgaaagg attgacactc ttaagttttg
     1081 aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt gccaaaagaa agatggtttt
     1141 tctttcttcc agagaatgta agtacattta tgttatttta tcttatagtt atgattcttt
     1201 taggaacatc agttatttct aaaaatacgt atacgattga tgaagcaggt tttcctgtgc
     1261 ttgtcagctt gtatgtggga ataggtttcc agaattttgt caatgcacga acagaaagct
     1321 tggctgtttt gatatttggt ctatttattg tttgggcaac tgatatcgga gcatatatgg
     1381 ttggtcgacg ttatggtcag cataagttaa gtcctgatat ttcgcccaac aaaacgattg
     1441 aaggtgcgct cggtggtatt ggtagtgctg ttgtagtcgc atttctttat ttattgctat
     1501 acccagcaaa agaactattt aatcatggta tgtttgtcat gcttctgatg acgattattt
     1561 tttcagcagt cggacaattt ggtgatttgg tcgaatctgc aatcaaacgt cattatgatg
     1621 tgaaagactc gggaaatatc ttaccaggcc atggtggaat cttagaccgt ttcgacagtt
     1681 tgctgtttgt atttcctatc atgcatttat ttggcattat ttagtaagta tacctgcgtt
     1741 tctttgtgaa tttcataaaa gacgcaggtt tttatttttc attatattga tactattggt
     1801 gtctttggta tactatggta aaattatagt gttttatcgt tgttttataa aaaacacaaa
     1861 aaacaagtaa aggagaaaaa agctatgaaa acgattctga catttatcat cgtttttggt
     1921 atattagtga ttgttcatga gtttggtcat ttcttctttg cgaaacgatc aggaatcctc
     1981 gttcgcgaat ttgctatcgg tatgggtccg aaaatttatg ggcatcaagc aaaagacggg
     2041 acaacatata cgcttcgcct attacctatt ggcggttatg ttcgaatggc aggcaatggg
     2101 gatgatgaaa cagaaatggc accaggaatg cctctttctc ttttactaaa ttctgacggc
     2161 atcgtagaga agataaattt aagtaagaaa atccaattga caaatgctat cccgatggaa
     2221 ttgagccgtt atgatttaga agatgagctg actatcactg gttatgtgaa tggagacgaa
     2281 acagaagtag taacctaccc tgttgatcac gatgcgacga ttattgaaaa cgacggaaca
     2341 gaaatcagaa ttgcgccaaa agacgtacag ttccaatcgg ctaaattgtg gcagcgtatg
     2401 ctgaccaact ttgctggacc catgaataac tttattttgg caatcctgtt attcatcatt
     2461 ctggcattta tgcaaggtgg tgttcaagtg acgaacacta atcgcgtagg agagatcatg
     2521 cctaatggag ctgcggcaga agcaggctta aaagaaaatg atgaggtagt cagtgtggac
     2581 ggaaaagaaa tccattcatg gaatgatctg acaacagtca ttacgaaaaa tccggataaa
     2641 acattagact ttaagatcga acgagaaggt caggtgcaat ctgtcgacgt gacaccgaaa
     2701 tctgtagagt caaatggcga aaaagttggc caacttggta taaaagcccc aatgaataca
     2761 ggttttatgg ataagatcat tggagggacg cgtcaagctt tcagcggttc attggaaatt
     2821 ttcaaagcct taggatcact atttacaggt ttcagtttag ataaattagg cggacctgtc
     2881 atgatgtacc aattgtcttc agaagcagca aatcaaggtg taacaactgt tattagtttg
     2941 atggcgcttc tttcaatgaa tctcggaatc gtcaatctgc ttccgattcc tgccttagat
     3001 ggcgggaaat tagtcttgaa tatttttgaa gggattcgcg ggaaaccatt aagtcaagaa
     3061 aaagaaggaa tattgacatt agctggcttt ggatttttaa tgttgttgat ggtgctagtt
     3121 acatggaacg atattcaacg attctttttc tagaaaaagt tgacaaatag ggtataataa
     3181 caaacgataa ctattttgag gaaataaagg agtatataga atgagacaat caaaaatgtt
     3241 gattccaaca ttacgcgaag ttccaaatga tgcagaagta ttaagccatc aaatcttgct
     3301 aagagctgga tatatccgcc aagtatcagc gggtatttat tcatatctgc cattagcaaa
     3361 tcgtgtattg gaaaaactga aacgcattat gcgggaagaa tttgaaaaaa tcggagcggt
     3421 agaaatgctg atgcctgcca tcttgccagc agaactttgg gaagaatccg ggcgttacga
     3481 aacatatgga ccaaacttgt atcgtttcaa agaccgcaat gatcgaaaaa tgatccttgg
     3541 accaactcac gaagaaactt ttactgattt gatgcgtaat gaaatcaatt catataagaa
     3601 attaccttta aatctttatc aaatccaacc taaatatcgt gacgaaaaac gacctcgctt
     3661 cggtttgctt cgcggaagag agtttatcat gaaagatgcg tattctttcc atgcaacgga
     3721 agaaagtttg gacgagacat ataaagatta cgaaaaagcc tacaccgaag tattcaagcg
     3781 ttgtggctta gaatttcgct cgattatcgg agacggtggc gcaatgggcg ggaaagactc
     3841 aaaagaattc atggcgatct cagaaatcgg ggaggatacg atctgttatt cgactgaaag
     3901 tgattatgct gcaaacttgg agatggctac aagctactat gtatcaaaaa aatcgcatga
     3961 aacacagctt gaattggaga aagtagctac acctaacgta aaatcaattg acgaagtagc
     4021 agatttcttt tctgttgaac ctcaaaaaat catcaagtct gtattgttca ttgcggatga
     4081 acaacctgtc cttgttttgg tacgcggaga ccatgaagtc aatgacgtaa aactgaaaaa
     4141 ctttttatct gctgactttt tagaagaagc aacagaagaa gaagcacaaa aatatctagg
     4201 tgctgagttt ggttctgtcg gaccagtcgg tgtttcagat gacgtaaaag tatatgcaga
     4261 ccgttatgtc caagatttag cgaatgccat tactggagcg aacgaaacag gattccacta
     4321 taaaaatgtg aatccagaac gcgatttcac tgtactttct tatgaagatc ttcgttttgt
     4381 acaagaagga gatccttcac cagacaacaa tggtgttctt gcctttactc gaggaatcga
     4441 aatcggacat atcttcaaaa tcggcactcg ttacagtgaa tctatgggag caactgtact
     4501 agacgaaaat ggacgagaaa aatttgtgat tatgggctgt tatggtatcg gtgtaagtcg
     4561 tttattatca gcaatcgtag aacaacattc agatgaacaa ggaatcaact ggccaaaagg
     4621 aattgcgcca tttgatttgc atatcgtcca aatgaatcta aaagacgaat accaaacaaa
     4681 cctgacgaat gaactagaag aagcgatgac aaaagctggc tatgaagtac tggttgatga
     4741 cagaaatgaa cgcgcaggag tgaaattcgc ggattctgat ttgattggct gtccaatcag
     4801 aatcactgtc ggtaaaaaag cagtggatgg aatcgtagaa gtgaaaatca agaaaactgg
     4861 cgaaatggtt gaagtacgta aagaagaatt ggtgaatact ttaccaatcc tcttgaacca
     4921 aggcgaataa taagcgttaa aacacagaaa ataaaaatag ctgaatcagc tgtttttgac
     4981 aatctgatga ttcgctttta aaattggaac tgcgatttga atcgcagttc caatttttta
     5041 atgtaaaact cagaaaaaac gagtatacta aaaggtactg aaaaagaaga ggataagatg
     5101 cgatcaattg cattgtttct catcagaaag ggcgtatatc tttgtcagaa aaacgagatt
     5161 tatttgaaaa actgttggaa caaatccaac tagaggaagc agaaaaaaat catccactgc
     5221 ttactgctgg tgaaatggat agtgtagtgg tccatcgtaa atcgcgttta tgggaattta
     5281 cgctgcattt ttccaaaatt ttgcctatta tgctatatcg gtcattgact cagcatctga
     5341 ctatcgcttt taaagatatt gcacaagtca aactgaatat cttagcagat gaccagacat
     5401 tcgatgaaca gctcttgcaa gattactggg cccaagcttt ggaaaatcag caatgcgata
     5461 caccactagc acagcaagta ttgaaaacgc aggtacctgt gataaaagat cgtaaagtca
     5521 tcttgccaat cgacagtaca ggagctattt cttatttgaa gcaacaatac ttgccattag
     5581 tagaagagtt gttcgtcagt tacggattcc caaaatttcg tatcgaacca gaagtggatg
     5641 agcagcaagc ggaacgagta ttgaaacttt ttgaagaacg caaacaagaa caggcagaag
     5701 cctttatgaa gcaagcagct gaaagtctag ttgtccacga acaaaagaaa aaggaaagaa
     5761 aagagcaatc gccagctctt gaaggaccca tccacatcca gctaggacga aatattccag
     5821 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5881 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5941 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     6001 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     6061 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6121 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6181 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggga
     6241 aatggggaca ccgagctatc gccattacag accacggtgg tgcgcaagcc tttccggaag
     6301 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6361 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6421 atgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6481 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6541 caggacatcc tttgtcacga acaacggtag atctgactgg tatcacggat ggaatggttc
     6601 gaggatcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6661 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6721 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6781 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaaatttggt gtcagcttgg
     6841 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6901 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6961 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     7021 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     7081 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7141 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7201 gaaaccgagc aaaattctac gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7261 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7321 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7381 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7441 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7501 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7561 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7621 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7681 cgttacctga gatcattcaa aaacgattaa aaaaagagtt gaattctatt aatggtaatg
     7741 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7801 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7861 ctgaagttaa tccgttagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7921 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7981 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     8041 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     8101 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8161 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8221 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8281 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8341 cgatccaata tccagcagac gatcaaaatt cggaatggaa aacgactcat tttgattttc
     8401 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8461 ttcggatgct tcaagattta tcagggatcg atccacaaac gatccctacg gatgacccgg
     8521 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8581 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgctag
     8641 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8701 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8761 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8821 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8881 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8941 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     9001 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     9061 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9121 aacaagcaat gaaagaaatc acggacaaag gattagatgc ttccgtaaaa gaaaagaatc
     9181 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9241 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9301 catttagagc tgtcccaagt ttgggaacca acgtggcaaa acaaatcgtt gaagcaagaa
     9361 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9421 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9481 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9541 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9601 ttttaatgga attacgcact taatttttta aggaggcgaa acttttgagt agcgtcgttg
     9661 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
     9721 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
     9781 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
     9841 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
     9901 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
     9961 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
    10021 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
    10081 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
    10141 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10201 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10261 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10321 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10381 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10441 gattttgggc ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10501 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10561 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10621 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10681 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10741 ccagacctat tgcgccgctt atttgagcaa gaagtaccag aagtctatga tggaatcgtt
    10801 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10861 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10921 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10981 gtattcattg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    11041 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    11101 cgcggacaaa acgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11161 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11221 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11281 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11341 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11401 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11461 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11521 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11581 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11641 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11701 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11761 ccgatgcaag cgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11821 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11881 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11941 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    12001 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    12061 cgattactag caatgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12121 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12181 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12241 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12301 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12361 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12421 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12481 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12541 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12601 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12661 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12721 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagtc gtaacgatta
    12781 tgggacacgt agaccatggg aaaacaactt tattggatac attacgccat tcacgtgtaa
    12841 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12901 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12961 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    13021 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    13081 taaataaaat tgataaacca ggtgcaaacc ctcaacatgt tatgcaagaa ttaagtgagt
    13141 atgagttaat tcctgaagca tggggtggag aaacgatctt tgttgagatc tcagcgaaat
    13201 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13261 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13321 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcatgtt ggtgacccaa
    13381 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13441 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13501 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13561 gcgcgaaacg tgcattatta gaaaaccgtg ctgccacaag ccgtgtgact ttggataact
    13621 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13681 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13741 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13801 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13861 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13921 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13981 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    14041 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    14101 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14161 tgggttatga atgtggtgca atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14221 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14281 cattatggct aactaccgtg accgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14341 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14401 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14461 agataagaaa acagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14521 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14581 tgtctatggc aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaac
    14641 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14701 acctaagagt tccatgactt tagtcttgcg gcgtgtcagc taaccaaaaa agagaaaaag
    14761 aaaagtaaaa aattcgactt tcttttttct agtagtatat aaatttttgc atatagataa
    14821 agatcaagat accgataacg gaagctagat tgtaatctat tttagtgaca gtcaggtata
    14881 gccaagtaac tagaagatca gtggaacctg caccataaaa gtttgagttt gtcggtccgt
    14941 tgacagttaa aagaaaaatc acgttgaagt tattgatatt tccgattacg ccgcttcgat
    15001 ttggtcacga tccaaatttt ggataatccc tgtagaaatt agcatagtag ctgggatacc
    15061 aattcacata ttgacaaaga taacagagac ttttacataa aacggatcaa tcaggaaagg
    15121 aattgctcga tccagcccca gttttccagc aatgtgttaa ctggcccagc gtcattcagc
    15181 aaattatgca tgatgaggag cgaaatgaac tgcggaacag ccattgtgat aacgacaagt
    15241 tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat ttcgtggttt tttatacctg
    15301 ttcatttggc tagatataaa atccaaacaa atgaacagaa gtgcttatta ggtttcaatt
    15361 gggagtatct gcgctagaat aagagtaata atgaaaagga gtgttatgta tggattattc
    15421 tagtaaagaa tactttgata aaatgacggc ttggtggcgt gctgctaatt atctttctgt
    15481 tgggcaactt tatcttaaag ataacccctt actgagacgt acactgaaac ccgaagatgt
    15541 caaaaaacat ccaatcggtc attggggaac gattcctggc caaaatttta tttatgtcca
    15601 tttgaaccga gtcatcaata aatatgattt gaacatgttt tatattgaag ggccaggaca
    15661 cggcggacaa gtcatggtat cgaatgctta tttagatgga agttacacag agatctatcc
    15721 agaagtaacg gaagatgaga caggaatgca aaaattattc aaacgatttt catttcctgg
    15781 aggcattgct tctcatgcag caccagaaac accaggttct atccatgaag gcggagagct
    15841 tggatactct ctttcacatg cagtcggagc tgtattagac aatcctgaag taatctctgc
    15901 tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta gctggaagct ggttctcaaa
    15961 tgtctttatc aatccagtga tagatggagc tgttttaccg atccttcatt taaatggtgc
    16021 aaaaatagcc aacccaacga tcttggcacg taaatcagat ggagaattag caaattactt
    16081 taacggactt ggctgggaac ctttctttat tgaaggaaat gattcagaaa aattaaatcc
    16141 tgttatggct gaaaaaatgg atcaagcaat cgagaaaatt aaatccatcc aaaaagaagc
    16201 tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg ccagtattga tcgtacgcac
    16261 accaaaagga tggaccggtc caaaagaatg ggacggggaa ccaattgaag gaaccttccg
    16321 agcacaccaa gtaccgattc ctgtagatca agaacatatg gatcatgcag atgctttact
    16381 ccgttggctg aaatcctatg aaccagaaaa attatttgat gcacaaggac gtattttaga
    16441 agaaatcaga gaaatcgcgc ctactggtga ccaaagaatg gcaaaaaatc ctatcaccaa
    16501 tggtggaatc gatccaagac cgcttattat gccagattgg aaaaaatata ctttgcaatt
    16561 tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa cttggtaaat tcgttcgcga
    16621 gatcattgag aaaaatccag aaaatttccg tatatttgga ccagatgaaa caaaatcaaa
    16681 ccgattgaat caagttttca aaacgacaaa tcgccaatgg atggaaaaaa tcgaaccaga
    16741 aaatgatgaa tggctttcgc catctggtcg cgtcatcgac agtcaattat cggaacacca
    16801 agatgaaggg ttcttagaag ggtacgttct gactggacgg catggttttt tgcaagttac
    16861 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatgcggaaa
    16921 tcacatgacc tttcttggcg aaatgattat ccttcgctaa atctcattgc aagctcaaca
    16981 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    17041 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    17101 gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa
    17161 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    17221 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    17281 gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc
    17341 ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt
    17401 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    17461 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    17521 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    17581 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    17641 aaaggaagtc aagcagaaga ttttgcgaaa aaaatggatc agaaattaca agaacatcaa
    17701 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    17761 cttgaccaat aaaatgataa aatcgatccc gcaaaataga attttttaca ttgattaaga
    17821 aactgaaaaa ataaataaaa aaaacggaaa aaagactgga agaatggcca gtcttttttt
    17881 tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa atccaatcaa
    17941 atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaagcat aacatgattt
    18001 ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa aaccgttcat
    18061 tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag ttaaatcaaa
    18121 aatgaaatgg agaaaagaca tggacgggtt attaccttta tggaaagaac gcggaatgac
    18181 aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa tcggacatgg
    18241 gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa aagccacaaa
    18301 agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa ctctaggttt
    18361 cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag tagatatgtc
    18421 tttatctgaa aaaaaaatag atgaagtgat ggcttcattt attggtgaga tcacccagat
    18481 accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat atgcccgcaa
    18541 taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta tccggacaag
    18601 tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag tagaatgcgg
    18661 gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg gttaccctgc
    18721 gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaagg atgctctgac
    18781 attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt tgctgccaat
    18841 agaaaaagga gtggaacggt tcaaacgagt agatattact gatgaagtat ggcagaaagt
    18901 aaaaaacggt atgagacttg attatactgt atttgcgctc ccagaaatgc cgtcagaaga
    18961 aattgctttg ttttataaag gaaaagttgt cagtatttat cagccaaacc cgaaagaaaa
    19021 aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga atatcatcaa
    19081 gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt tagtgctggg
    19141 atttttcgac ggcgtgcata tcggacacca gaaagtcata aagacaggta tggaaattgc
    19201 acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat ctattgtctt
    19261 taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa aagaagaaaa
    19321 aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt cttttgctca
    19381 tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa aatatgctgt
    19441 ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc attttcctga
    19501 atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac aaggtgaaaa
    19561 aatcagctct acccgtattc gtggattact agacagcgga caagtaaaag aagcagcaaa
    19621 actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg cacgaggcag
    19681 actactagga ttccctacag caaatgtcaa agtaaagagc acggttcatt tgcctaaaga
    19741 aggtgtttat gtgtcggaaa taaaaattgg ggaaacatgg tatccagcca tgggttcaat
    19801 tggacataat gatacattcg gtgaaggacg ggagctgact gtagagttgt atattttgga
    19861 ctttaatcaa gatatctatg gtgaacatgt cgcggtacgc tggaatcaat ttttaagaga
    19921 tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaggagg atgaacaagc
    19981 aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt gtttatgtat
    20041 aactagcaat tacaataaaa tgggtgataa ataagcccag gaaaggggct gtgacaaaag
    20101 tcttgtcaca gccttttttt ccaaataaat ggtttttcag aagtagcttc ttcggaaata
    20161 agccgaaatt tcttgaagct gaccacttct gtcacaacct ctttctttat agaaataaaa
    20221 ggacactagc aattcatgaa aacgattgct agagcgtatc atttgcttta gactatatat
    20281 cttggtgtac actcacttta aggcggaaat aaaagttatc cgtaataaca ggtttaggta
    20341 tgaaaaagga gtgggtacta tgtacaagga attagaaaac aaagtcgcag tagtaactgg
    20401 tggctcaaag gggattggaa cagcgattgc tcaacgattc gggaaagaaa aaatgaatgt
    20461 agttgtcaac tataactcgg ataaagaagg agcagaaaaa gcagtcaaag caatcgaaga
    20521 ggcaggcgga aaagcagtct ctgtacaagc agatgtcagt tctgaagaag gaatacagcg
    20581 tttacttgat acggctttag aaaattttgg gacactggac gtttgggtta ataatgctgg
    20641 gatggaaaat cagcatccaa cccatgaact tagccttgaa gattgggaaa gagtgttaaa
    20701 agtcaatttg actggcgttt ttatcggaac gaaagcggct ttaaattact ttacagagaa
    20761 taataaaaaa ggaaatatca tcaatatgtc ttctgttcat gaacagatcc catggccaac
    20821 gtttgctcat tacgctgctt caaaaggcgg ggtaaaatta tttaccgaaa cggtagcaat
    20881 ggagtatgca ccgaagggaa tcagaatcaa tagtataggc cctggtgcta tccgtacacc
    20941 tattaatgcg gcaaaatttg atgatcctga acaaaaagaa ctcttggaaa gtatgatccc
    21001 actaggacgc gtaggtgaac caaaagaaat agcggcgaca gctgcatggt tggcttcaga
    21061 cgaatccagt tatgttacag gtattacact atttgcagat ggtgggatga ctttatatcc
    21121 ttctttccaa ggtggaaaag gataagggtg aataaaaatg ggtttattga ttgcattgat
    21181 cccggcagtt ggctggggga tccagccact gattttgaaa aagattggtg gcagaccaac
    21241 aaatgagata ttaggaactg gtattggagc ggtgcttgtc ggcttactcg ttcaattatt
    21301 cgcttctcca gctggtattt cagtgactac atttttgatt agtttacttt ctggtgcatt
    21361 ttgggttatt ggacaaacag gacagtatac atctttcaac ttgattggtg tttctaaaac
    21421 gatgccaatt tcgactgcat tacagttagt cggaacctcg ttgattggtg tcttcgcttt
    21481 tggagaatgg tctggaagta tggggaaaat cattggagca gcagcaatca ttcttcttgt
    21541 atttggttct gctttaacgg caatcagtga tgatcctgga aaaaaaggcg gtatcacaaa
    21601 aggaattagt ctattagcat caacaagtat tggttattgg gtatacagtg cattgcctaa
    21661 actagttgat gcaagcggac tttccatttt ctttccgcaa atgatcggtg tctttttagg
    21721 ggcagtgatt tatgtgattt tccgtaaacc aaccgcattt tctgaagaca agagttggaa
    21781 agcaacgatc gttggagcca tctttagtgt gtcagcattg gcttatattt tttcagcaaa
    21841 tgaaaatggg gttgctactg catatatcat tacacagcta aacgtagtta tctctacatt
    21901 aggcggtatg tacatcctgc atgaaaataa gacaccaaaa gaaaaaaaat taacgattat
    21961 cggattattg ctgatcgtag taggaagcat ggtcacggtc tttatttgag gataaagatg
    22021 gtaaaatttc atttattaaa aaaatatttg tttgaaccaa acagtaagta cttataaaaa
    22081 aatagcgcta gaatttccaa catctctgga atttctagcg ctttttaatt gtttaagata
    22141 caagggaaat atctgtctgg atcaataatt tttcattgat ccattgacta aagtttacga
    22201 cataattgaa gttaccaccg attagtatct ccgcatgatt gtctttataa agatcgtatt
    22261 gtaaaagacg gtctttttta tttgcgtaaa catcgttatg gtactggact aattgttctc
    22321 ggaattgatt gattgctgta aaatcacgat attgaaggtt gaaacgcatc gtgattggtg
    22381 catttagcat gaatcgctgc atttcttttg ctgcttcctc tgcttgatct ccgtatagga
    22441 actcaaaaag attacgttca gaaaatttcc attgcccgcc aacctttgtt ccttttagtt
    22501 ttccttcatg aagataattt ctgatagaac gtgtagtaac acctaattgt tcggctaatt
    22561 catttactga gtaaaaatct ttcaatgttc accctcctat gtgaatggtt ctactgactt
    22621 agttgatatt tgcttaatca cgaaatgtag aaagaacgat tttaattaag gtcattgtaa
    22681 aaatcaagtc agaaaattct aacttttatt tctctaaaga aattcgttct ctcctaaagt
    22741 gatattatat ttaaatatac ctgaaaataa tgggcagtat tatgaaagat cactgatatt
    22801 ttctcgtatt ttttccttat tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta
    22861 ctatgtagaa aattcaaagc cattcaatca gtgatttttt tcagcacaag a
//
LOCUS       DANJJW010000033        22854 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000033 DANJJW010000000
VERSION     DANJJW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            62..286
                     /gene="nrdH"
                     /locus_tag="PWS82_001227"
     CDS             62..286
                     /gene="nrdH"
                     /locus_tag="PWS82_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL0906371.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            445..2604
                     /gene="nrdE"
                     /locus_tag="PWS82_001228"
     CDS             445..2604
                     /gene="nrdE"
                     /locus_tag="PWS82_001228"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL0906372.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2654..3619
                     /gene="nrdF"
                     /locus_tag="PWS82_001229"
     CDS             2654..3619
                     /gene="nrdF"
                     /locus_tag="PWS82_001229"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL0906373.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3708..4847)
                     /locus_tag="PWS82_001230"
     CDS             complement(3708..4847)
                     /locus_tag="PWS82_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0906374.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5156..5869)
                     /locus_tag="PWS82_001231"
     CDS             complement(5156..5869)
                     /locus_tag="PWS82_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL0906375.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6123..6824)
                     /gene="nagB"
                     /locus_tag="PWS82_001232"
     CDS             complement(6123..6824)
                     /gene="nagB"
                     /locus_tag="PWS82_001232"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL0906376.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7058..8590
                     /locus_tag="PWS82_001233"
     CDS             7058..8590
                     /locus_tag="PWS82_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0906377.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8781..9983)
                     /locus_tag="PWS82_001234"
     CDS             complement(8781..9983)
                     /locus_tag="PWS82_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906378.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10065..10754)
                     /locus_tag="PWS82_001235"
     CDS             complement(10065..10754)
                     /locus_tag="PWS82_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0906379.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10860..11228)
                     /gene="rplL"
                     /locus_tag="PWS82_001236"
     CDS             complement(10860..11228)
                     /gene="rplL"
                     /locus_tag="PWS82_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL0906380.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11281..11784)
                     /locus_tag="PWS82_001237"
     CDS             complement(11281..11784)
                     /locus_tag="PWS82_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL0906381.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11988..12115)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12194..12883)
                     /gene="rplA"
                     /locus_tag="PWS82_001238"
     CDS             complement(12194..12883)
                     /gene="rplA"
                     /locus_tag="PWS82_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL0906382.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12987..13409)
                     /gene="rplK"
                     /locus_tag="PWS82_001239"
     CDS             complement(12987..13409)
                     /gene="rplK"
                     /locus_tag="PWS82_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL0906383.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13752..14420
                     /gene="sdaAB"
                     /locus_tag="PWS82_001240"
     CDS             13752..14420
                     /gene="sdaAB"
                     /locus_tag="PWS82_001240"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL0906384.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14433..15305
                     /gene="sdaAA"
                     /locus_tag="PWS82_001241"
     CDS             14433..15305
                     /gene="sdaAA"
                     /locus_tag="PWS82_001241"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL0906385.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15396..16415)
                     /locus_tag="PWS82_001242"
     CDS             complement(15396..16415)
                     /locus_tag="PWS82_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0906386.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16588..17493)
                     /locus_tag="PWS82_001243"
     CDS             complement(16588..17493)
                     /locus_tag="PWS82_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0906387.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17552..18097)
                     /gene="nusG"
                     /locus_tag="PWS82_001244"
     CDS             complement(17552..18097)
                     /gene="nusG"
                     /locus_tag="PWS82_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL0906388.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18198..18368)
                     /gene="secE"
                     /locus_tag="PWS82_001245"
     CDS             complement(18198..18368)
                     /gene="secE"
                     /locus_tag="PWS82_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL0906389.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18392..18544)
                     /gene="rpmG"
                     /locus_tag="PWS82_001246"
     CDS             complement(18392..18544)
                     /gene="rpmG"
                     /locus_tag="PWS82_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0906390.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18706..19350
                     /locus_tag="PWS82_001247"
     CDS             18706..19350
                     /locus_tag="PWS82_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0906391.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19406..19909)
                     /locus_tag="PWS82_001248"
     CDS             complement(19406..19909)
                     /locus_tag="PWS82_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0906392.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19922..19968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20506..21471
                     /locus_tag="PWS82_001249"
     CDS             20506..21471
                     /locus_tag="PWS82_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0906393.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21570..22742)
                     /locus_tag="PWS82_001250"
     CDS             complement(21570..22742)
                     /locus_tag="PWS82_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906394.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22729..>22854)
                     /locus_tag="PWS82_001251"
     CDS             complement(22729..>22854)
                     /locus_tag="PWS82_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906395.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7144 a         4587 c         4109 g         7014 t
ORIGIN      
        1 tactaagagt tttatactat ctattataga taataagaat gtgttagagg aggcactcac
       61 catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt
      121 tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt
      181 agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac
      241 agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa
      301 tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt
      361 ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat
      421 acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc
      481 aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa
      541 gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttctatct
      601 attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa
      661 ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct
      721 ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac
      781 ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg
      841 aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag
      901 ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt
      961 ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg
     1021 caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc
     1081 gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc
     1141 gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat
     1201 ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat
     1261 gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa
     1321 ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat
     1381 ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca
     1441 aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa
     1501 caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc
     1561 gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct
     1621 gtgattaacg gcaaacagga atatgaagta ttaggaacgg atatctcatg taacttaggc
     1681 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
     1741 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
     1801 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
     1861 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
     1921 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
     1981 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
     2041 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
     2101 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
     2161 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
     2221 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
     2281 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
     2341 atggacatgc gtaaagtaat cgatgtttat gcaatggcac aacaacacgt cgatcaaggc
     2401 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
     2461 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
     2521 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
     2581 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
     2641 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
     2701 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
     2761 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
     2821 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
     2881 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
     2941 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
     3001 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
     3061 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
     3121 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
     3181 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
     3241 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
     3301 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
     3361 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
     3421 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
     3481 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
     3541 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
     3601 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
     3661 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
     3721 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
     3781 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
     3841 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
     3901 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
     3961 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
     4021 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
     4081 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
     4141 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
     4201 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
     4261 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
     4321 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
     4381 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
     4441 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
     4501 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
     4561 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
     4621 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
     4681 taatagataa aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat tttcaatatt
     4741 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
     4801 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
     4861 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
     4921 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
     4981 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
     5041 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
     5101 atttggctgg gcttattact tgatgtagga catctatttt acaacctcag atcatttatt
     5161 gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca
     5221 catcggcata agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt
     5281 agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa
     5341 aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt
     5401 tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa
     5461 aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg
     5521 cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc
     5581 ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac
     5641 gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct
     5701 caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac
     5761 ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga
     5821 tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct
     5881 ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta
     5941 cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa acggtgttca
     6001 atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta tttttccttt
     6061 gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc tactattgaa
     6121 aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc gtgattttgc
     6181 aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt gattgcatca
     6241 gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat tgaaccgatt
     6301 cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg tttgtttgca
     6361 ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga agcaccaggt
     6421 tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc gattggatgt
     6481 gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc ttttccatct
     6541 ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat gaaatgacgg
     6601 tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac agaagtcatt
     6661 tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg tgtgcttcct
     6721 gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat ttcaaatgct
     6781 ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat tgctccttta
     6841 tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat tagtatagac
     6901 caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag gtttcataag
     6961 atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca ctgtggacgc
     7021 tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga cattaaacaa
     7081 cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc
     7141 tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat tgattaaaat
     7201 catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa atattcacaa
     7261 aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag aatttctaaa
     7321 aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt accaaacata
     7381 tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa cggaattaga
     7441 ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg ggttggggat
     7501 cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac gttcaaaggt
     7561 gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg aaccaaccaa
     7621 tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt ttgacggtgc
     7681 gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc atatcgcaga
     7741 catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat tgaaacaaaa
     7801 ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac aaatcgaaaa
     7861 gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg caaaaagccg
     7921 acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt
     7981 ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa ctgatttagt
     8041 catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct ttggtgaaaa
     8101 tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa cactgatcgg
     8161 tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa tcaattactt
     8221 ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt taagcagtca
     8281 atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag ggttagttaa
     8341 tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta aggtgaaact
     8401 tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga cgaaccatat
     8461 cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag gaacagtaat
     8521 catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca ttgagattga
     8581 accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa agttttgtca
     8641 caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt agctcaaaaa
     8701 acggcaaaat atgaggagct atttttattt ggctattctt attacttgat gcagaacagc
     8761 ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc tttaaaaaca
     8821 actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt tacacctaac
     8881 cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc tccaataaag
     8941 tacataaaca taaatacagt attatttcgg ttgcttgctg cttcacctag cccttgtact
     9001 cgtgtttgat tggcgacttg accaaattga ttcccgacgt ctaataaaat aatagctaaa
     9061 agcaaaatgg ccaaatggct tccgccaacc aataaagcaa taaagcttat tgtttgcata
     9121 acaagtccca tatacacgat ttttctttca gaataagaat ctgccagtcg tcccacgata
     9181 ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata tccctgcttc gaatgttccc
     9241 caattgtagg cgggactgct gatataaaaa atcaacgtag accaaaagat cgaaaatgtt
     9301 ccaaacatga aaaatccact gactgccgct tctcttaata gacgttgaga agcaactaaa
     9361 tgcggcaaac ttttaagtga atcaaggtat gaaacagctg tactactttg ctggacagaa
     9421 cgcggcattt tccagtaaag cagaagtgta agaattccga ctgcacataa agcaaataaa
     9481 tagatcatcc gccaagaaaa aagactggca acaataccag ataccgtccg agaaagcaaa
     9541 atccccacga gtaaaccgct aagcaatgta cccatgacct tccctcttgc ttttggtccc
     9601 gctagtaccg caccgtaagg aatgatgatt tgcggcacga tcgaaagtaa tcctattaaa
     9661 aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa aagcggccaa cagagagacg
     9721 gcagataacg ctgccattcg aatgatcaat tttggacgat ccaccacgtc ccctaaagga
     9781 actaaaaaca gtaaccctaa cgcatagcct aattgggtca acatcgtcaa tatcccgact
     9841 gcacttgtgc taacagataa actagctgct acttttgttc caatcggctg gatgtaatac
     9901 atattggcaa caacaacgcc acaagtgatt gataatagta aaataagcga agttgttaaa
     9961 ggtttttttt cattactctt cattgatttc ccccatttcc tatcttagtt tacaacttat
    10021 ggaaaagatt ggaaatagaa aaatacaact attcgccaca cttattattt gtgcttttgt
    10081 tttccttgtt ttttcttgcg atctttataa atgaagacaa ctaaccctac tagcacagta
    10141 acacttgcaa ccaacgtgac tgcagttttg ttatatccgt gaaacagagc ttctccacca
    10201 cacgcataca acacagacga agggatgact ccaatcacga tcgacaatat gatttgctgt
    10261 agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag aagatggaat aaccggtatc
    10321 atatagccta acattacacc tatttttgga tgtttcattt gtcggattgc ttgcacccaa
    10381 tgattcgttt cggttttctt atctaaaaat tttaagcgat gcataaggaa aattgcagtg
    10441 atgttaccaa gagcattacc caaaatgttg atgatcgcac ctgtcagtgg tccaaagctc
    10501 aaaccagcaa tgactccaac tactgaagta ggaatacctg gaacagcaca catcaccgct
    10561 gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg aacgaatatg tgcgagtagc
    10621 tctgccttac tcgctctcgg atttaaaaat aattggatgt ctgtctgata ttctaaatac
    10681 aaccgatagc ccactaccag tatcagaatg attcccaacg agatcaatat caatcggatt
    10741 attttctttt tcatatttcc tcctgaaaaa aatctatatt cattaaataa tagtttaaca
    10801 taaaaacaaa aaagcaccga aatccaacat aggatctcag tgcttttaaa tagtaaaaat
    10861 tatttaacag ttacagatgc gccaacttct tcaagtttag ctttcaattc ttcagcttct
    10921 tctttagata cgccttcttt aactggtgca ggagcgccat caactacagc tttagcttct
    10981 ttcaagccta aaccagttgc ttcacgaact gctttgataa ctttaacttt ttggtctcca
    11041 gctgcagtta attctacagt gaattctgtt tgttcttcag cagcagcagc gccaccagca
    11101 gcagctacag ctacaggagc agcagcagat acgccaaatt cttcttcaat agctttaact
    11161 aggtcgttaa gttctaggat agttgcttct tttaactcag caacgatgtt ttcaatgttc
    11221 aatgccatta taatttcctc cgtttaattg ttctttttta tttaatgctt tatgcggcaa
    11281 ttaagctgca tctccatctt ctttgtctgc cactgctttg acagcgtaag ccacgttgcg
    11341 gactggcgct tgtagtacag atagcaacat agatagaagt ccttcgcgat ttggcaattt
    11401 tgccaatgct gtgatttctt ccaatgaaga gactttacct tcgatgatac cgcctttgat
    11461 ttctaatgct tttgcttctt tagcaaattc gtccatgatt ttcgctggtg cgactacatc
    11521 ttcgttactg aatgcaacag ctgtaggtcc agtgaatact tcgtccatgc cttcaaggcc
    11581 agctttttta gctgcacgtg aaagaataga atttttgata actttcattt ctacgttagc
    11641 atcacgaagt tgtttacgta aacgtgttac ttcatcaact gttaatccgc ggtagtcaac
    11701 gactactact gatgcagctg ctgtaaattt ttcgcttact tcatcaacga ttgctgcttt
    11761 ttttgcgata attgcttcac tcatttttga gtttcacctc ctgaattttg atggaagagt
    11821 tttttgcact gaaaaggcaa gtcactttta tagttacttt gctcaaccag tattattcat
    11881 cacctattca cttcgttcat agtgattcta ctcgctttgc tcgaaaggaa actcctttcg
    11941 ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt tcatagtgat tcacaataaa
    12001 aaactctatg ccaccgcaga catagaggga ctattgaata tattcaataa acgtcctcgg
    12061 taggaaatta aggcttgatg ccacctactg tcttcggtac aagttaatta ttaacctcaa
    12121 ctagcttagc atgctaaact agttgagtca agaattattt ttcagttgga tcaatcaaca
    12181 ttctaatatt taattagaaa gaagcttggt caacatggat accaggtcca aatgttgttg
    12241 tgatcgttac atttttcatg tattgacctt tagctgctga tggtttagct tttagcaata
    12301 catcatggat tgctgcaaag ttttcaacta atttttcatc gtcaaatgaa acttttccga
    12361 ttggtacatg gatatttcca gctttgtcca cacggtaagt tactttacca gctttcactt
    12421 cgtttactgc ttttgttacg tccattgtaa ctgtaccagt tttagggttt ggcattaagc
    12481 cttttggtcc taatactcgt cctagacgac ctacagtagc catcatatca ggtgttgcaa
    12541 caactacatc aaagtcaaac catccgtttt ggattttttg aaccatgtcg tcgtcaccta
    12601 cgaaatctgc accagctgct tcagcttctt ttgctttgtc tcctttagca aaaactaaaa
    12661 cagtttgtgt tttaccagtt ccgtttggta atacaacagc accacggatt tgttggtctg
    12721 cttttttagg gtctacgttc aaacggtatg caacttcaac tgttgcatca aattttgcaa
    12781 tgtttgtttc ttttgctaaa gctactgctt cggcaacagg ataaacttta cttgaatcaa
    12841 ctttttttaa tgcatcttgc atttttttgc tctttttagc cattttggtt cctccttgat
    12901 tgtggttata acggttgtac ctcccacgtg ttctatatct ttcaatatag agccaactga
    12961 gaagctgctg tcttagggtg tatagattat tctacagtga tccccatgct tcgtgcagtt
    13021 ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt ttaggtcttc catttttaat
    13081 tcagcgattt ctcttacttg atcgctagaa actttcgcaa ctttattttt gtttggttcg
    13141 cctgaacctt tttcgatttt agctgctttt ttcaatagaa cagcagctgg cggtgttttt
    13201 gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa caaccggaat gatcaaacct
    13261 gcttgatcag ctgtacgagc gttgaattct tttgtgaatc ccatgatatt gatacccgct
    13321 tgacctagtg caggacctac tggtggagct ggagttgctt tacctgcagg aatttgcaat
    13381 ttaacgattt tttctacttt ctttgccacg agacatacct ccttaagtcc gtgatgtggt
    13441 taattggaga tgcatatttc tcctcccacg ttttatctga tctgaaaaaa tccagacaaa
    13501 taatgtgcaa aacaatgcac actggtacag tatagcaaat ttatttgttt tttcaagatt
    13561 tttttctgct atttttacat tttgcttgaa taaacgctaa atttattcta aaaaaacaac
    13621 aatttttcgt tctcatttct tcttttatgt tacacttgcg ctagcatggt tcaacagatg
    13681 aaagcaaaca ataacacggt tttttagacc cttgtacaaa gattcctaca taacaaaatg
    13741 aggtggagaa aatgaaagat ttaaggtata gaagtgtctt tgatattatt ggacctgtga
    13801 tgattggtcc aagtagctct cacactgccg gagctgctag aatcgggaaa atcatacgta
    13861 gtatatttgg cgaacagcct gattctgtag atatttatct atatgaatca tttgcaaaaa
    13921 cctatcgggg gcacggaaca gatattgcat tagttggcgg cctgttaggg atggaacccg
    13981 atgatgaacg tttagctgat tctttgaaat tggcatatga agccggtatg gaagtcttgt
    14041 ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt aaagatgctt ctaaaaaaag
    14101 gaacacgaaa gctttcagtc actggcattt cgatcggcgg tggcaatatc cagatttctg
    14161 aattgaatgg attcaaaatt tctttaagca tgggtactcc taccttgatc gttgtccacc
    14221 aagatgttcc aggtatgatt gctaaagtga ctagtatact ttctgagacc aatacgaata
    14281 tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa tgccattatg ataattgaag
    14341 tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa acagctgcca aatatcgaca
    14401 gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt atcgattgaa gaattagtag
    14461 atcaagcaaa agcttatcca agtgtagcag aattgatgat tgcagttgaa atggaaatga
    14521 gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa aaacctctca gtaatggaac
    14581 aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac aggaattacc ggtggtgatg
    14641 caaaaaaatt agatgattat ttaaatgagg cagacttttt aagtggagaa acaatcttaa
    14701 aagctgtacg caatgctgta gctgttaatg aagtcaatgc aaaaatgggg ttgatttgtg
    14761 ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt gctgatggct gtccgagatc
    14821 ggcttgattt gacacatgaa cagcaattgg aattcttatt taccgctggc gctttcgggt
    14881 tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg gggatgtcaa gcagaagttg
    14941 gttctgcaag tgcaatggca gcagcagctt tggtttgtgc aaaaaatggg actcctcatc
    15001 aagcagcaca agcagtagcc attacattga aaaatatgat gggactgatt tgcgatcccg
    15061 ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc tttaggctct tctcaagctt
    15121 ttatctctgc tgatatggca ttagcgggta tcgaaagtgt cattccgccg gatgaagtcg
    15181 ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat tttcaaagaa acagcagaag
    15241 gtggcttagc tgttacgcca actgctaaac gacttgcgga agagatttta aataagaggg
    15301 cgtgataaag ctccttaatt tcgagtatta agagattagt tatcttcata acaaaaaacg
    15361 aagagaggac taattgccct tctcttcgtt ttttattaat ccatcaattt cgtatttttt
    15421 acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga tcgacaattc tttgttcagg
    15481 tgttcaaaaa cagatgttac tccttctgcc ccacctagat tcaacccata aatcactggt
    15541 cggccaatcg caactaagtc agcaccactt gccagagctt tgaagacatg ctctcctcga
    15601 cggacaccag agtcgaaaat cactggtaca cgtttgttga ctacttcggc aatttttggc
    15661 aatacttcaa aggacgcagg acctccatct agttgacgtc caccatgatt ggatacccag
    15721 ataccatctg cccctgcaga gatagcgatt actgcatctt ctggcgattg tattcctttt
    15781 acgataactg gaagatgggt gatttctttg attgttttga tgtcatctgg tgtcaagcct
    15841 tgttttgcag ctgcataaat ttctgcgatt ccttttcctt cgccattacc acttgcactt
    15901 tgttcgctat acgcagctaa gttaggcatt ggtaatggaa attggaattg attgatgacg
    15961 tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta aaatgatagc tttagcccct
    16021 gcttctactg ctttgttcaa aatgaactca ttgaatctgt cgtctttact catatataat
    16081 tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt ctttgattgt cgtatttgca
    16141 taagtgctga tggaaaagat tgatccagca gcagctactc ctttagctgt atcagcttcc
    16201 cctttttcat gtgccaatcc ttgggccgct gatggcgctt ggataatcgg tgtatctaat
    16261 tcaattccga aaacagatgt atgcaaatcg gctgagtcga ttccgcgtaa tatccgtggc
    16321 atgattttct ttgtattaaa agaagtcgta ttttctttca tcgtccactc atcttcagaa
    16381 ccaccgcgaa tatagccaaa agctcctgct tccatgcgct cttttacacg accttctagt
    16441 taggcaatgt ttacaatatc gattggatgt tcttcattac ttgtatgata agtcatagta
    16501 gatccttctt tcatcttctt gattacatat aaatcatact aaacttacaa aaagtaagca
    16561 aacatttact ctcagatact tttatcgcta ttctttccaa ataaatcgac ttacaagctg
    16621 atctacttct tggttttcat gaagcatact atgagaggca ttttcacctg tcactacttt
    16681 ttcctctatt tcatttccgc gttgcttcaa caacgcatag accgctaatg cactgtttaa
    16741 cggtactgtt ccatcactca agtctgtttc atctagttga ccagcaatca agaaaaatcg
    16801 agtggttata ggaatattac cgatcatttg ctggtaatct tgatagcggt tagaaacgac
    16861 agcaggcccc tttgctaact catctgtcag agactgcgca ctatcatcga caaaatcatt
    16921 aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc aatgacgtat cctgtccata
    16981 cgttcctaaa tagcgtagtg cactaactcc tcccatggaa tgccccacta tgttgacttt
    17041 atctatgccg tagttttctt tcagatacaa aagacaagtt ttgatccatt ctgcttgatt
    17101 ccactcgtta tttttattat cttcaaacaa tacttggacc atcggattgt ctgcttttcc
    17161 agacaaatct cctgttgctt gtatttgtcc atctgttcct actgttaaaa ccaattcttt
    17221 tactgcttgc tgattttttt ccaaacgccg caacatccct ttaaaggaat tttctgtccc
    17281 gctgtagccg tggaaaaaaa gtgttggaat agtggttttt tctatcactt tactggattc
    17341 tgccgttttt tctttatcag aaacttcagt agtatttatc ttcgtcgtct ggcaaccact
    17401 taacgcaaac aaactgataa ggactaaagc tgctgcagtt gcacgtttgc ataaattttt
    17461 gattggttct gtcatacacc tcttcctttc catatagaaa aaagaaagac ttgaaaggcc
    17521 aatcatatga tcgacatttc aagtctcttt attaatcgat tttatctact tgatcgaaat
    17581 caagttctgt actggtttca cgtccaaaca tatcgatgtt gactttcaat ttttcttttt
    17641 ctccgtcaac ttctgttact tgtccttcta atccagcaaa tgcgccttcg atgattttca
    17701 cgacttcgcc aacttctact tctagatcat tttgtcgcag gctcataccg atagaacgca
    17761 agatatgatt gacttcttct ggaagtaatg gtgctggttt gcttccggct ccgtgtgaac
    17821 caacgaatcc tgtcacacca ggtgtgttgc ggacaacata ccatgaagcg tctgtcatga
    17881 tcatttcaac aagtacgtat cctgggaatg ttttatgaac gatttctttt tctttcccat
    17941 ttttgacttc agtttctttt tcttcaggaa caacgacacg gaaaataaag tcggccattt
    18001 tcatactttg cgcacgagat tcgatatttg cttttacttt gttttcatat cctgaatatg
    18061 tatgcaagac ataccagttt ttttcaaaag attccattga aggctcctca tttcattgta
    18121 aaataaaaaa acctcagtac acccgaagtt tttctttcgc ttcattatag cactaaaagt
    18181 acgttgtggc tagcgagtta ttttaaaatc cagccaaata agctttggat ggctgtatcc
    18241 attacgtaaa acatcaatcc gaaaataata gaagtttcga tgactactaa cgtatcttta
    18301 cggagttgtt ttctgcttgg ccaagttact tgtttgattt catcttttac gctttttaga
    18361 aatttcatga gacaacctcc cttgctactg tttatttcgt ttctttatgt aatgtatatt
    18421 gattacaata tttacaaaac ttattgattt ccaaacgttc gccgcttttt ccttcactga
    18481 ctgattttgt gtagttgcga gacccacaaa cggaacaagc gagtgctgct tttttctttg
    18541 ccatttactt ctgccccctt gctgagaatc agacttatac ttcataacag tatcacgtaa
    18601 ctttttactt gtcaatttgt gaaataaact atctattgtt ttggataaaa ttatgatatg
    18661 attaatttga aattaagcta aaatgagaca attggaggta tagcgatgct attaaaaaca
    18721 cttgtatata agaaaaaaga tctgacaacc gttacagaga ctagtacatt agaagaagca
    18781 ttaaaaattt tagaaacttc cggataccgt tgtgtgccga tcttagattc ttcaggtaat
    18841 attttccgtg gaaacattta caaaatgcat atctatcgtc ataaagcaaa tggcggtgat
    18901 atgacgcttc ctgtcactca tcttttgaaa aacgctacaa agttcattta cctggatact
    18961 tctttcttca aagtattctt tacgatcaaa gagcttcctt atattgctgt ccttgacagt
    19021 gaaaatcatt tttatggtat tttgacacat agtacgttgc ttaatatgct ctctcaatct
    19081 tggagcgtag aacaaggaag ctatgtactg acaattgctt ccacaggaaa gcaaggagat
    19141 ttggctgcta ttgcaaaaat catttcaaaa cacagcagca ttgccagctg tattactctt
    19201 gatatcgaaa aagacgaata cattcgacgt acattgatca cgctaccggc taacactgaa
    19261 gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa agaattataa agtggtagaa
    19321 attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg aaaaaagaaa acagctaatc
    19381 tgctttcttt tttcgctttt tataattaga cacgcttttc aaaatcaagc gcgcgatcta
    19441 aataatacat caatggtgct gaaacagcca tgacgataaa ctcacttaat gcaagcgtgc
    19501 cataggttgt ccaaaatgct tggccacctg ttggaactaa catataagca atcagtccca
    19561 tgctgatggt aaagaagatc gtgttcaata ctaaacgcat ctttacattt ttcactttgt
    19621 tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg gccgccacca aaaaccacat
    19681 ccatgatacc aaaaccaaag aaggcattat agacgatgac cccgcctaaa actccccaaa
    19741 gcaatttacg gttaaagaca actaaatgat tcaaactttc tgaaatacga aactggatcg
    19801 gccctgagga tacaggcgca actaaaatag ttaacgctag ataaagagcc atgatgatgc
    19861 catttacaac gattacggat aaccgttgtc tgcttttcgt tttttgcatt ttctctcctc
    19921 ttttctccta gtttttttaa cgcgggatgg ttgatgaacc gcggttatgt gaaacaaaca
    19981 ataattataa cagacttttc atttatttca atccgtctta tctgctttta aaaaagcaaa
    20041 agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata tggtataaag taagtgtaag
    20101 gggtcataag aattcccagt aaagcagacg aagtaggatc tgcaagaaca gaattgttgt
    20161 gaaacttaaa taaataaaaa aggatgtggg aatttgagca acctcttact agagaacggt
    20221 ctagcaagca gctgaagatc taagtggaca tgccgaacct tggaacttct ccttacgtaa
    20281 gtaatggatc atgcgcttgc taggcttttt tgtgtttttc ggtcttcgga cctttttcat
    20341 tttcctagac ttctgctata ataaccaaga attaattttg taacagattt ttttgaaaaa
    20401 acaagtttct ttcattcaaa aaatagaaaa agcttgctaa ttattggtta accagcaaac
    20461 aggagtatta aaatatcttg ttttattgaa tagcaggagg aaatcatgaa attccattat
    20521 catatattga tcaatccatc ggcaggcagc ggaaatggct ataaagtagc agaaagaatt
    20581 ttaccggtat taaaaaataa gcatattgac tatacacttc attatagtga atacaaagga
    20641 cacgatgctg agattgctga aactttggca aaagaaacat tgattccttg gatagaagaa
    20701 gaacagaaaa caatcgatac tttccccctt ttgatcatcg taggaggtga cggcacctta
    20761 catcaggtac ttgatacttt ctatcagatg gaagtagaat tcccagttgc ttatattcca
    20821 gcaggttctg gagatgattt tgctcgaggg gcaggccttc caaaaaatcc caaaaaagga
    20881 cttcagctga ttttagcagc tcaatcacca gaaaaagtcc acataatggc ttatgaagaa
    20941 aaaataagtg aaaaaaaagg aatagctgtt aataatttcg gtataggatt agatgcagct
    21001 atcgtccatg cgacaaatca ttcttccaca aaaaagcgat tgaacaaata caacttagga
    21061 tcattttcgt atttattctc tattctccgt gctttattca ctcaaaaagg atttccaatt
    21121 ctggtagatg caggaggaaa acaattaagt ttttcaaacg catttttatg tacagcaacg
    21181 aagcatcctt tttttggcgg aggaatcgcg attgttccaa ctgctgatgc gtctaaacca
    21241 gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta aaatcttgtg gctcattctt
    21301 ttacttatac agaaaaaaca attgaaatcg aaatatttcc atcactacac aacaaacaga
    21361 ttacgtatcg tttcaacaac accgcaacat ggtcaagaag atggagaaga aatggaaaaa
    21421 cgaccttttg atatcacaat aaccacaact aaacagttgt tttggtgtta ataaaagagg
    21481 ttttgtcaga agctacatct ggaacaccgt ttattcggaa aaaaggactg tgacacgact
    21541 tttgtcacag tccttttttt actatgcagt taattctttg cccaattggg ttgccatcga
    21601 tagtttcttt ctttacccaa aataaattgg atcattgaaa acaccgcagc aaaagcaact
    21661 aagacacaac cattgaacca aaaagattct gcaaatgttt gattgtaact gagtgtttca
    21721 ctgatcaagt tattattttg aacaaaccaa tatgttggtg tgaatgccgc tattttgcta
    21781 accatttctg gcaagtattc tgctggtaca aacaccccac cgataaagca actgcctgtg
    21841 ataaaaatat ctttgatccc gccgctggtt tctgagtttt ttaccaatga agtgatacaa
    21901 gccgaaaagc taatgatcgg caataaaaac aagaaagtat ttaaaatatg ataccatacc
    21961 gttatatccc acgaggtgtg gatcactaac agaaccatta gaagtaagaa gccactgaac
    22021 aacagcagcg agtaactaat caaactaaag gttactttgc gattcaagtt tcgtttggta
    22081 atgggcgcac tctggtttcg tttactgatc tctttgcgat taaatgctag attgatgacg
    22141 ctgaaaccgc taaaaatgga tagaaacaac ccataagaca agaaattaaa ggttgtgccc
    22201 gtcaacagct ttttcatccg ctgactatat cccgcactta gctcaacttc tccagtaacc
    22261 gagagtgcca tttgtgtctg ttccatagct gcatcgattg ttttatcttc ttcttgaaac
    22321 agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa caactgtttt agaaaaggca
    22381 tctgggcgac tctgtatctt gatcgtcggc atctttccta cttcgacttg ttcggtaaag
    22441 tctgcaggca gatgtaagat aatgtttaca cgattaaaat aaagagcatc atcgatttct
    22501 ttttgtgagg ttccatccaa tgtaacgact gtttccttgt cattcaaata tttacgaagg
    22561 ccatcgacta caggcgactc ttcttcacta atcaaggcga ttttcgcatc ttgcagagag
    22621 gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg caacaacagt gataattaca
    22681 cctatcagta aattcgattt atttgcttta agcaatgtcc aaatcgtttt ataaagcgtc
    22741 ataacgcacc cctctttcca tccaagcatt tcctaacgca aaaaggaaaa taaatccgat
    22801 caaccataga agattggtat aaaaactagc caaatcagtg tagaaataaa gttt
//
LOCUS       DANJJW010000034        22635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000034 DANJJW010000000
VERSION     DANJJW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(7..441)
                     /locus_tag="PWS82_001252"
     CDS             complement(7..441)
                     /locus_tag="PWS82_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906396.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(551..2410)
                     /locus_tag="PWS82_001253"
     CDS             complement(551..2410)
                     /locus_tag="PWS82_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0906397.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2528..3688)
                     /locus_tag="PWS82_001254"
     CDS             complement(2528..3688)
                     /locus_tag="PWS82_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL0906398.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(3995..5419)
                     /gene="araA"
                     /locus_tag="PWS82_001255"
     CDS             complement(3995..5419)
                     /gene="araA"
                     /locus_tag="PWS82_001255"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL0906399.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5435..6133)
                     /locus_tag="PWS82_001256"
     CDS             complement(5435..6133)
                     /locus_tag="PWS82_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0906400.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6090..7751)
                     /locus_tag="PWS82_001257"
     CDS             complement(6090..7751)
                     /locus_tag="PWS82_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL0906401.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7969..9369
                     /locus_tag="PWS82_001258"
     CDS             7969..9369
                     /locus_tag="PWS82_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL0906402.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9621..10700)
                     /locus_tag="PWS82_001259"
     CDS             complement(9621..10700)
                     /locus_tag="PWS82_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0906403.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10786..11745)
                     /locus_tag="PWS82_001260"
     CDS             complement(10786..11745)
                     /locus_tag="PWS82_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0906404.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11757..13268)
                     /locus_tag="PWS82_001261"
     CDS             complement(11757..13268)
                     /locus_tag="PWS82_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0906405.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(13290..13853)
                     /locus_tag="PWS82_001262"
     CDS             complement(13290..13853)
                     /locus_tag="PWS82_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL0906406.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(13859..14722)
                     /locus_tag="PWS82_001263"
     CDS             complement(13859..14722)
                     /locus_tag="PWS82_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906407.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14736..15632)
                     /locus_tag="PWS82_001264"
     CDS             complement(14736..15632)
                     /locus_tag="PWS82_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906408.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15807..17096)
                     /locus_tag="PWS82_001265"
     CDS             complement(15807..17096)
                     /locus_tag="PWS82_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0906409.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(17068..18585)
                     /locus_tag="PWS82_001266"
     CDS             complement(17068..18585)
                     /locus_tag="PWS82_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0906410.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18783..19673
                     /locus_tag="PWS82_001267"
     CDS             18783..19673
                     /locus_tag="PWS82_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL0906411.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19746..20570)
                     /gene="nadE"
                     /locus_tag="PWS82_001268"
     CDS             complement(19746..20570)
                     /gene="nadE"
                     /locus_tag="PWS82_001268"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL0906412.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20582..22054)
                     /locus_tag="PWS82_001269"
     CDS             complement(20582..22054)
                     /locus_tag="PWS82_001269"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL0906413.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         6519 a         4807 c         3832 g         7477 t
ORIGIN      
        1 tttcttttat tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc
       61 tgtcgcttgg tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc
      121 aagatcgata ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc
      181 aaaattttta tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcttaag
      241 cattgcccga tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga
      301 tttgtccagt tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg
      361 atgcattcct tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata
      421 atagccaata ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat
      481 tctacttgtt tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag
      541 ctcgccattc ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga
      601 caatgtcata ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt
      661 gcgcttcttt ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt
      721 cctgttctaa tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca
      781 ttagattcgc tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta
      841 attctccttt actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt
      901 ttgcttggat cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag
      961 tccttctaga aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg
     1021 aaatatcttg cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag
     1081 gatagtctag attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat
     1141 gaatgaactg aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact
     1201 catcagaaat atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga
     1261 tgaactggtc aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt
     1321 atggatggat cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa
     1381 gctgatccat ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg
     1441 ccatagaaaa accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt
     1501 gagaaagcat attgccacta tatgtttgat cagctacttc ataataatct gccaatggct
     1561 caccagatac ttctacgccg tttaatcgac taggatctga gaaactttca ctcatcacga
     1621 acacgatgtt cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt
     1681 atttttcagt gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt
     1741 ctaccttcag attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct
     1801 ggctgtaagg tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat
     1861 tgttgaaatt actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga
     1921 tcaattggaa tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta
     1981 tcacggttcc tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca
     2041 gcaatccgat ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac
     2101 tcattttcat ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac
     2161 ctaaagggac agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac
     2221 aggctaagaa gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg
     2281 atagatcatt ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac
     2341 tgatgattgc tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat
     2401 atcttttcaa atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt
     2461 tagttgtacg aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc
     2521 tctttcttta tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt
     2581 tttctagctg ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca
     2641 atatgtagac atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct
     2701 cattcagcag agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga
     2761 atcgaaacgg cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg
     2821 taccgaacaa aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag
     2881 cttcctgagt cgatccccct agatgaggca acataagtat acggtcattt tctaagaact
     2941 cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa
     3001 cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca
     3061 ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga
     3121 ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca
     3181 actgggcatc gcggatagaa taacccaaga catccatccc taagctatag caacttagcg
     3241 ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc
     3301 gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg
     3361 tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa
     3421 caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt
     3481 cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca
     3541 aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg
     3601 gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa
     3661 tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa
     3721 atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag
     3781 agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt
     3841 ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat
     3901 tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt
     3961 tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact
     4021 caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat
     4081 gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt
     4141 tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta
     4201 cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt
     4261 ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag
     4321 gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg
     4381 attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg
     4441 tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc
     4501 cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt
     4561 gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta
     4621 agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct
     4681 tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat
     4741 cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat
     4801 caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt
     4861 ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg
     4921 caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga
     4981 cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag
     5041 cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat
     5101 attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt
     5161 ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga
     5221 cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg
     5281 gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta
     5341 cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt
     5401 tttctccaat attcaacata attagccact ccttttattt gttttgtccg taatacgcat
     5461 tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa
     5521 atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt
     5581 tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta
     5641 cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc
     5701 ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta
     5761 ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt
     5821 gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg
     5881 aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga
     5941 tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc
     6001 cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg
     6061 tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca
     6121 gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca
     6181 atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct
     6241 gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct
     6301 aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca
     6361 ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc
     6421 tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca
     6481 ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg
     6541 atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt
     6601 ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg
     6661 caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt
     6721 atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc
     6781 gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg
     6841 tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa
     6901 gtaccagccg aaatgttccc tgtccgtttg gctacactgt tcgtagcgac cataccggtt
     6961 cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg
     7021 atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta
     7081 ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata
     7141 tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca
     7201 tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt
     7261 gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg atacaaatga
     7321 gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct
     7381 tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca
     7441 aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc
     7501 tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc
     7561 caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa
     7621 ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat
     7681 ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt
     7741 acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt
     7801 agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagcg gttatttaca
     7861 agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac
     7921 aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa
     7981 aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga
     8041 tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca
     8101 ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc
     8161 ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct
     8221 gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat
     8281 tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt
     8341 cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat
     8401 caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaagtg
     8461 ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt
     8521 ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc
     8581 ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa
     8641 ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact
     8701 ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag
     8761 caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact
     8821 gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct
     8881 ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata
     8941 ggcggtagcg tgatgggact ttcattcatc ctaccagctg ttttaggaac tgtactggat
     9001 gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct
     9061 tgcacatggg ctccactgac atgggtaatc ataggcgaaa tattcccact agctgtccgc
     9121 gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg
     9181 ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc
     9241 atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct
     9301 ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat
     9361 cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta
     9421 gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga
     9481 cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag
     9541 ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag
     9601 aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt
     9661 acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac
     9721 cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc
     9781 cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga
     9841 tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg
     9901 atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct
     9961 cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca
    10021 cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg
    10081 aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc
    10141 acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc
    10201 atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta
    10261 cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac
    10321 tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct
    10381 cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg
    10441 gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca
    10501 tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg
    10561 attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag
    10621 ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca
    10681 atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa
    10741 cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg
    10801 aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc
    10861 gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata
    10921 gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa
    10981 ttgcttattt ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt
    11041 taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt
    11101 tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc
    11161 atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt
    11221 cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat
    11281 ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc
    11341 gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt
    11401 cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg
    11461 tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga tataatggac
    11521 ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag
    11581 gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta
    11641 gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc
    11701 tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac
    11761 attttcttta ttctaattac attccaagag tatttttcta tccctacatt taattcgtca
    11821 tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta
    11881 tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg
    11941 aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc
    12001 agctgattgc cgttatctac gatcacactg tccacataag gaacattttt tatcttttca
    12061 caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat
    12121 aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt
    12181 attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt
    12241 cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac
    12301 caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca
    12361 ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa
    12421 tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat
    12481 gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta
    12541 tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata
    12601 cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca
    12661 attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct accatattca
    12721 taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat
    12781 ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct
    12841 tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa
    12901 ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt
    12961 ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct
    13021 ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga
    13081 atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca
    13141 tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca
    13201 acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt
    13261 actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata
    13321 gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg
    13381 agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat
    13441 actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc
    13501 acatatatcc aggataaaag aaacatggaa taaataagta aaactacata tccactaatt
    13561 gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt
    13621 aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa
    13681 atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta
    13741 gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg
    13801 taatcagaaa ccacaatcaa ctgtccataa aatttagtct caactatttt catcttcctc
    13861 actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa
    13921 ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa
    13981 tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg
    14041 tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg
    14101 ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta
    14161 atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca
    14221 tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa
    14281 gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca
    14341 tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa
    14401 atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa
    14461 tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat
    14521 ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt
    14581 taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca
    14641 tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg
    14701 taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt
    14761 gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac
    14821 agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga
    14881 acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac
    14941 acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga
    15001 ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca
    15061 tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa
    15121 ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat
    15181 tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca
    15241 agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat
    15301 gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa
    15361 ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa
    15421 ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt
    15481 gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg
    15541 acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca
    15601 ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata
    15661 aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt
    15721 ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa
    15781 cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct
    15841 gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg
    15901 atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag
    15961 tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct
    16021 actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt
    16081 gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc
    16141 ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt
    16201 ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc
    16261 ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa
    16321 atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta
    16381 aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca
    16441 tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat
    16501 acatttttac tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta
    16561 ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac
    16621 atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata
    16681 gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt
    16741 tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca
    16801 ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg
    16861 atatccattt caattttgat gtttttatca tttgttttat taaattcatt aacgatttct
    16921 tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta
    16981 tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct
    17041 accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga
    17101 taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc
    17161 ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt
    17221 tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc
    17281 agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag
    17341 ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt
    17401 cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc
    17461 ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat
    17521 tggtgcaata acgtttacca attgagcaag acaagcaatc tttactctat ctgcatgttt
    17581 caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc
    17641 tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata
    17701 ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt
    17761 gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc
    17821 aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc
    17881 tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg
    17941 ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt
    18001 agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg
    18061 accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt
    18121 cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt
    18181 acgagctgca tctactccac gagtacccag attgacagcc atattgattt cggcccccac
    18241 atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca
    18301 agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa
    18361 tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa
    18421 gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat
    18481 cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac
    18541 tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta
    18601 atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt
    18661 acttattttt tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt
    18721 aatataactt ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat
    18781 atatgcaact acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa
    18841 caaggatcaa gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa
    18901 aagaacttgg agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa
    18961 acatcatcaa aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa
    19021 tcgatattag ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac
    19081 ctattggtca ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg
    19141 attttatcgg gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc
    19201 gcattctctg gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag
    19261 aggatacatt ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa
    19321 atgacaattg gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga
    19381 cgagccctgg agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata
    19441 atcttaatca atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga
    19501 atggagaacc cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc
    19561 atttgcgaat cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta
    19621 aaggattcgg taattacgat caggatatca aattaaaagt ttactacagc tgatattttt
    19681 cttttctatt ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt
    19741 tattgttatt tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc
    19801 ttgttccacc atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga
    19861 taatcatcaa tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc
    19921 ccatcttcaa gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct
    19981 tgaagcaatt gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca
    20041 ccatcaccaa atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat
    20101 acggctcctg ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg
    20161 atatttcctt tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt
    20221 gcatcaacag ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga
    20281 gcagcttttg catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat
    20341 tgatagtttt catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg
    20401 cctgctaaag tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga
    20461 tattttttca aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca
    20521 attgttggtt tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct
    20581 cctatgcttc tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt
    20641 tatggttcca gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt
    20701 tatactcttc ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg
    20761 caggtaattc gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg
    20821 taaagtctcg aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt
    20881 cttcttctcg agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa
    20941 aatttcgagt gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc
    21001 ttgataactt gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga
    21061 atactgcacc aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata
    21121 catcaatttt agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg
    21181 aagcatagat ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag
    21241 atatataggc catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta
    21301 attctttcgc cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga
    21361 ctaagaacac gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc
    21421 cataagattg gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc
    21481 cagcacgtac attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga
    21541 ctgctgcatc cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga
    21601 tgacagattt gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca
    21661 acagtgctgt ttctactaat tggcagtgcg ctaaaggccc ttccacttgg atcaatggtt
    21721 catttgcaaa aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat
    21781 tttgtaaata ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat
    21841 cggaatcatt gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa
    21901 ccgcgtatcc gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat
    21961 cagctcgacc taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca
    22021 gcgtcaaact gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt
    22081 tcatttccta cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct
    22141 gaaaaagcac atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta
    22201 tcctttctta ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc
    22261 gagacacgaa gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc
    22321 tactacaatc gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac
    22381 aacataatca atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta
    22441 tccccaaaga aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg
    22501 acaagatata ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga
    22561 tacaaagtaa gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag
    22621 tctcgccttg ttttt
//
LOCUS       DANJJW010000035        22613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000035 DANJJW010000000
VERSION     DANJJW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(65..217)
                     /locus_tag="PWS82_001270"
     CDS             complement(65..217)
                     /locus_tag="PWS82_001270"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0906414.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(408..1286)
                     /locus_tag="PWS82_001271"
     CDS             complement(408..1286)
                     /locus_tag="PWS82_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0906415.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1325..2443)
                     /locus_tag="PWS82_001272"
     CDS             complement(1325..2443)
                     /locus_tag="PWS82_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL0906416.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2440..4539)
                     /locus_tag="PWS82_001273"
     CDS             complement(2440..4539)
                     /locus_tag="PWS82_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL0906417.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4590..5411)
                     /locus_tag="PWS82_001274"
     CDS             complement(4590..5411)
                     /locus_tag="PWS82_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0906418.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5408..6217)
                     /locus_tag="PWS82_001275"
     CDS             complement(5408..6217)
                     /locus_tag="PWS82_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0906419.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6238..6732)
                     /locus_tag="PWS82_001276"
     CDS             complement(6238..6732)
                     /locus_tag="PWS82_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0906420.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6744..7232)
                     /locus_tag="PWS82_001277"
     CDS             complement(6744..7232)
                     /locus_tag="PWS82_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0906421.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7309..10005)
                     /locus_tag="PWS82_001278"
     CDS             complement(7309..10005)
                     /locus_tag="PWS82_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0906422.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10433..11506)
                     /locus_tag="PWS82_001279"
     CDS             complement(10433..11506)
                     /locus_tag="PWS82_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0906423.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11503..12330)
                     /locus_tag="PWS82_001280"
     CDS             complement(11503..12330)
                     /locus_tag="PWS82_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906424.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12331..13137)
                     /locus_tag="PWS82_001281"
     CDS             complement(12331..13137)
                     /locus_tag="PWS82_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906425.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13149..14234)
                     /locus_tag="PWS82_001282"
     CDS             complement(13149..14234)
                     /locus_tag="PWS82_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906426.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14251..14793)
                     /locus_tag="PWS82_001283"
     CDS             complement(14251..14793)
                     /locus_tag="PWS82_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0906427.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15051..16040
                     /locus_tag="PWS82_001284"
     CDS             15051..16040
                     /locus_tag="PWS82_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0906428.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16037..16774
                     /locus_tag="PWS82_001285"
     CDS             16037..16774
                     /locus_tag="PWS82_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL0906429.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16771..17322
                     /gene="coaC"
                     /locus_tag="PWS82_001286"
     CDS             16771..17322
                     /gene="coaC"
                     /locus_tag="PWS82_001286"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL0906430.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17338..17970
                     /locus_tag="PWS82_001287"
     CDS             17338..17970
                     /locus_tag="PWS82_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0906431.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18055..18702
                     /locus_tag="PWS82_001288"
     CDS             18055..18702
                     /locus_tag="PWS82_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0906432.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18707..19690
                     /locus_tag="PWS82_001289"
     CDS             18707..19690
                     /locus_tag="PWS82_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL0906433.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20093..21607)
                     /locus_tag="PWS82_001290"
     CDS             complement(20093..21607)
                     /locus_tag="PWS82_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL0906434.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21745..22551
                     /locus_tag="PWS82_001291"
     CDS             21745..22551
                     /locus_tag="PWS82_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906435.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6831 a         4851 c         3916 g         7015 t
ORIGIN      
        1 ctttttataa tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa
       61 tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa
      121 tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat
      181 tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag
      241 aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac
      301 ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt
      361 actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta
      421 tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat
      481 tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca
      541 acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa
      601 ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag
      661 gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt
      721 ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt
      781 cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg
      841 aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg
      901 aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc
      961 gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca
     1021 aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat
     1081 ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta
     1141 aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa
     1201 ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct
     1261 ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac
     1321 ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt
     1381 ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat
     1441 catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt
     1501 gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg
     1561 cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat
     1621 accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat
     1681 gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact
     1741 cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc
     1801 aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact
     1861 tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc
     1921 tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat
     1981 ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat
     2041 aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct
     2101 ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc
     2161 catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat
     2221 cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag
     2281 tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc
     2341 ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac
     2401 tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat
     2461 gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg
     2521 cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc
     2581 ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa
     2641 tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc
     2701 cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc
     2761 taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc
     2821 taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc
     2881 aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt
     2941 tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc
     3001 taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa
     3061 attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg
     3121 tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa
     3181 ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca
     3241 ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc
     3301 aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc
     3361 aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc
     3421 taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa
     3481 ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt
     3541 actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg
     3601 atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga
     3661 tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct
     3721 gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg
     3781 acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa
     3841 atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc
     3901 ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat
     3961 cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg
     4021 atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta
     4081 aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac
     4141 aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc
     4201 gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt
     4261 taaggataac gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc
     4321 agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt
     4381 tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg
     4441 atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa
     4501 tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa
     4561 aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt
     4621 cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg
     4681 aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct
     4741 tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc
     4801 atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga
     4861 atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct
     4921 aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata
     4981 attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca
     5041 cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt
     5101 ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc
     5161 gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta
     5221 tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc
     5281 atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata
     5341 gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga aattccatca
     5401 gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat
     5461 cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa
     5521 ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa
     5581 caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca
     5641 tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct
     5701 ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg
     5761 gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc
     5821 ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa
     5881 aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa
     5941 taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca
     6001 aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag atggcttcta
     6061 gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag
     6121 caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaattccaa
     6181 taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta
     6241 aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac
     6301 ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat
     6361 cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc
     6421 cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac
     6481 attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat
     6541 cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt
     6601 catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt
     6661 tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac
     6721 tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc
     6781 tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc
     6841 tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag attaaagcca
     6901 gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca
     6961 cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca
     7021 aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc
     7081 tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg
     7141 tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata
     7201 taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa
     7261 aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat
     7321 atacggtcct aataattcat agagcagcga aagctcataa gcattgactt taaaattata
     7381 tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg
     7441 tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat
     7501 catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata
     7561 gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag
     7621 aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa
     7681 gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc
     7741 atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc
     7801 cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa
     7861 ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg
     7921 acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat
     7981 gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc
     8041 atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc
     8101 aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg
     8161 tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac
     8221 ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg
     8281 catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct
     8341 gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac
     8401 ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag
     8461 cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc
     8521 taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct
     8581 gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga
     8641 tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc
     8701 cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt
     8761 gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg
     8821 tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa
     8881 gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt
     8941 taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt
     9001 cagcttacca atattcccac taaagggatg agataacatt aaagacaagg cttctcttga
     9061 aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc
     9121 taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg
     9181 ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg
     9241 aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat
     9301 aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag
     9361 atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc
     9421 ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc
     9481 acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc
     9541 aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact
     9601 gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact
     9661 tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac
     9721 cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt
     9781 atcttccact aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt
     9841 cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa
     9901 aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata
     9961 ataaactatc ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac
    10021 acaaatagtc gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt
    10081 tttatctaat atgattttta tgttttttca tagctacaaa tattgaactg gattcatata
    10141 ttagttttat taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt
    10201 atccttctgt aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa
    10261 tggttttgtc cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa
    10321 aaagcggcgt ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga
    10381 tttttagctc tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc
    10441 tatacatctt gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat
    10501 ctttgtagac ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg
    10561 aaatgtcttt tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact
    10621 ctgcattttg tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt
    10681 ctttattttt cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg
    10741 gtgggatcac ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa
    10801 atgtcactgc cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga
    10861 ttgctttgac atttggtgtt aaatgattca atttatttgc tgcttctgtc aactgtgcca
    10921 tattcttact attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg
    10981 caccgtcaat cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct
    11041 taatttgatt tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat
    11101 atggaatcga atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat
    11161 tatttaatcc tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt
    11221 tttggatcat atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt
    11281 ttgtgaacat cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt
    11341 tttcaaattt cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta
    11401 cctttgctcc actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg
    11461 caataatgat gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac
    11521 cccctcgcgt actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa
    11581 gtagtatcca atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat
    11641 ttctaaactg attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc
    11701 atttcctgtg acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa
    11761 atatccagct atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa
    11821 attattagca ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt
    11881 tggcaagacc atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact
    11941 ctgaaaacct aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat
    12001 aacatcagga gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg
    12061 ttttttcgtg taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc
    12121 agataagaaa gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa
    12181 aacagcttca taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt
    12241 gaaggaataa aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa
    12301 ataaagatac gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc
    12361 tgtcaggagc atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat
    12421 cccccagttt tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg
    12481 atttccgcca atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg
    12541 gacaccactt ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa
    12601 gctattcgcg cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc
    12661 attgaaaatt ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact
    12721 gaagtctgtg aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac
    12781 gctgccgtgc acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca
    12841 tgtaggcaaa ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata
    12901 agcagtcggg taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga
    12961 attgatcgtc atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt
    13021 gaattgccca ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac
    13081 gaataaaccg agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct
    13141 tgtcccccct attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg
    13201 aaacgcatga catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta
    13261 gcttttttcg ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca
    13321 taatggacac cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt
    13381 gtcgtgatcg tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc
    13441 ccgccatcga cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt
    13501 acaccgtcca cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca
    13561 tcataaatat ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc
    13621 tcatcactca ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct
    13681 aatcgttgtt gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc
    13741 gccgataacg gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc
    13801 gctacacgct gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga
    13861 agctggacca tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc
    13921 ttgatcttca atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa
    13981 tcttggaaga ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct
    14041 tcaaaataga tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt
    14101 gttttaccac agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa
    14161 cttacttttt ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg
    14221 attggtttcc ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa
    14281 tacttttgtt cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata
    14341 gatcgactgt cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc
    14401 ttctaataca aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc
    14461 accttcaaaa gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca
    14521 ttccaattca tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac
    14581 cacttgcaga ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc
    14641 aagaatagaa aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat
    14701 ataccctttt gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat
    14761 gcgcaaattt cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa
    14821 tagtaaactt cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa
    14881 ccctttgtaa aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt
    14941 ttgattcttt atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga
    15001 gtaggcttta tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt
    15061 ttcgatcttt tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat
    15121 ttactgagtt ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata
    15181 aagatgcatt ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag
    15241 ggattgattt atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc
    15301 ttgtacttgc tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc
    15361 aaaaagtacc aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga
    15421 tgatccttgg tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag
    15481 gtatgacctc cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag
    15541 cgatagccct gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag
    15601 agtctgtcac atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc
    15661 tgccagagac aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag
    15721 gtggcgagca gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat
    15781 gtggcaatgc tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca
    15841 tccagttgat tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc
    15901 aacagatggc ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc
    15961 aattgcctga aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa
    16021 atgtaggtga ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga
    16081 tcaagtacgt gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta
    16141 tttagctgcc aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc
    16201 gaaaagacac tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca
    16261 attagaagaa ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag
    16321 tgattttact cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc
    16381 ttcaacgcaa gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt
    16441 ttcaaagatc tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat
    16501 catttcttat ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt
    16561 agatgtacca aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac
    16621 tgattttatt ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct
    16681 cttatctaaa gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat
    16741 tactgagaaa attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga
    16801 agtatttctg cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag
    16861 gtagatgtga ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct
    16921 ctgtcgaagc gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat
    16981 catatcgaac tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt
    17041 atcggcaagc tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt
    17101 ccagtgggaa caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg
    17161 atcactcagc gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga
    17221 gaatctcttt tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc
    17281 atcgaacagg tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg
    17341 aaaaatacaa aaaactttac attaactgcg atgtttttag ctattttgat tttattagct
    17401 gttacgcctt taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct
    17461 gtaatcattg cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt
    17521 ggactgatca gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct
    17581 ccgtttatcc cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt
    17641 attcctcgca tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta
    17701 atgaaacaaa aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc
    17761 aatacgattt tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc
    17821 atcggaacaa atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt
    17881 atacctgaag gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga
    17941 tacttcaagc acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt
    18001 ctcttttggt gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa
    18061 atttttgttg taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca
    18121 agttcccatg aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa
    18181 gagaatgttt cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca
    18241 ttcaaaggca tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa
    18301 gtagacttgg atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc
    18361 tatctgatgg tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca
    18421 gattattata ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt
    18481 gtcattcttc aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact
    18541 aagcctaatg agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc
    18601 gctctagtgg aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa
    18661 gagttgaata cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc
    18721 aatcaagcat cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc
    18781 aaaacaaggc gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat
    18841 gaaaagagaa tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg
    18901 agaggtagtt gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg
    18961 attagtcaat ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct
    19021 tctccctgat acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc
    19081 ttatcaaaca ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc
    19141 aggagcaagt ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa
    19201 aatcatcgtt actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga
    19261 tgaattgatc aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg
    19321 tcggggagtc gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc
    19381 ttctatagcc gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc
    19441 tgagatagac cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac
    19501 acctagaagt gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc
    19561 ttttctagaa aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt
    19621 caaacaagcg catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat
    19681 aactgaataa aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga
    19741 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg
    19801 aaggaatttc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
    19861 ttacttttgt cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt
    19921 aataagtcca accaagttaa aaacataaga agctgttttt acttggctgt tcttattact
    19981 tgatgcaggg cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc
    20041 ggtattagct caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat
    20101 tttccgaagc aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg
    20161 caactgaatt tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag
    20221 catataaccc cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt
    20281 tcacaccgcc catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag
    20341 acgataagtc atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa
    20401 catccgtatt ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt
    20461 ctgctaattc ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa
    20521 tagctgtggc acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca
    20581 atgctgtaat tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc
    20641 cctcttgatc gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct
    20701 catcctgctg cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta
    20761 atttttcaat catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat
    20821 cagctaaatc gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg
    20881 taactacgat tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca
    20941 cttcattgac cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag
    21001 ggatttcccg ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg
    21061 agccatcaga cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt
    21121 ccagtttgat accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt
    21181 atcgaagcat ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg
    21241 catcattcga gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac
    21301 cgcttgatga tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat
    21361 tcaacccagc atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa
    21421 tcacgacatc ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc
    21481 ctgatgatac ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat
    21541 ctgcgccgca tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt
    21601 ttttcatttg tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc
    21661 aactctgttt ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa
    21721 ggatatacat gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca
    21781 aaaactgcag gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt
    21841 atgctagtgg atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca
    21901 ttaattttat ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat
    21961 atgctagaaa tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt
    22021 gatttttatg gctatttttc aatgtgggat acttttctac atgcattcag cggattttta
    22081 gctgggggtg tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac
    22141 ttgactcctt tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg
    22201 tgggagtttg tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg
    22261 atcatccagc aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt
    22321 ccagatatca ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca
    22381 atcatccaaa atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt
    22441 aattttattg gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac
    22501 caaaaattta cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta
    22561 aaaaacaaga gaaggggcag tgacaatact tttgtcactg ccccttcttt ttt
//
LOCUS       DANJJW010000036        22241 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000036 DANJJW010000000
VERSION     DANJJW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(38..1189)
                     /locus_tag="PWS82_001292"
     CDS             complement(38..1189)
                     /locus_tag="PWS82_001292"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL0906436.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="PWS82_001293"
     CDS             complement(1202..1639)
                     /locus_tag="PWS82_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0906437.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="PWS82_001294"
     CDS             complement(1654..3420)
                     /locus_tag="PWS82_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL0906438.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="PWS82_001295"
     CDS             3684..4361
                     /locus_tag="PWS82_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906439.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="PWS82_001296"
     CDS             4364..5491
                     /locus_tag="PWS82_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906440.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="PWS82_001297"
     CDS             complement(5691..6131)
                     /locus_tag="PWS82_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0906441.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="PWS82_001298"
     CDS             complement(6142..8175)
                     /locus_tag="PWS82_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0906442.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="PWS82_001299"
     CDS             complement(8177..9880)
                     /locus_tag="PWS82_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0906443.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWS82_001300"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWS82_001300"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL0906444.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="PWS82_001301"
     CDS             complement(10963..11988)
                     /locus_tag="PWS82_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0906445.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="PWS82_001302"
     CDS             complement(12197..13066)
                     /locus_tag="PWS82_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL0906446.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="PWS82_001303"
     CDS             complement(13525..15417)
                     /locus_tag="PWS82_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906447.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="PWS82_001304"
     CDS             complement(15414..17141)
                     /locus_tag="PWS82_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906448.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="PWS82_001305"
     CDS             complement(17157..17807)
                     /locus_tag="PWS82_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0906449.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="PWS82_001306"
     CDS             18011..18307
                     /locus_tag="PWS82_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0906450.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="PWS82_001307"
     CDS             18479..19099
                     /locus_tag="PWS82_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL0906451.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="PWS82_001308"
     CDS             complement(19191..20357)
                     /locus_tag="PWS82_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0906452.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="PWS82_001309"
     CDS             complement(20369..20722)
                     /locus_tag="PWS82_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0906453.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="PWS82_001310"
     CDS             20850..21872
                     /locus_tag="PWS82_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL0906454.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="PWS82_001311"
     CDS             21891..22052
                     /locus_tag="PWS82_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906455.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
BASE COUNT         6447 a         4573 c         3945 g         7276 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc a
//
LOCUS       DANJJW010000037        21989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000037 DANJJW010000000
VERSION     DANJJW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            218..1114
                     /locus_tag="PWS82_001312"
     CDS             218..1114
                     /locus_tag="PWS82_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL0906456.1"
                     /translation="MASLVIIVTGVLDDKYELTPKQKSIGILIASLIIYFVADIRIDS
                     FTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAAGISLIGLITIGIIGYFF
                     LHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALFLGFMMAVLSLQGLKNVT
                     FVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLHHRLISLGFTHKGAVLTI
                     YAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIGLINEKHQFILKTLRFLG
                     NKEYRTQVKQKYLKKWNRHIDE"
     gene            1107..1907
                     /locus_tag="PWS82_001313"
     CDS             1107..1907
                     /locus_tag="PWS82_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0906457.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1907..2734
                     /locus_tag="PWS82_001314"
     CDS             1907..2734
                     /locus_tag="PWS82_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0906458.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2712..3446
                     /locus_tag="PWS82_001315"
     CDS             2712..3446
                     /locus_tag="PWS82_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0906459.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3546..4412
                     /gene="rfbA"
                     /locus_tag="PWS82_001316"
     CDS             3546..4412
                     /gene="rfbA"
                     /locus_tag="PWS82_001316"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL0906460.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4426..4998
                     /gene="rfbC"
                     /locus_tag="PWS82_001317"
     CDS             4426..4998
                     /gene="rfbC"
                     /locus_tag="PWS82_001317"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL0906461.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5020..6048
                     /gene="rfbB"
                     /locus_tag="PWS82_001318"
     CDS             5020..6048
                     /gene="rfbB"
                     /locus_tag="PWS82_001318"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL0906462.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6146..6997
                     /gene="rfbD"
                     /locus_tag="PWS82_001319"
     CDS             6146..6997
                     /gene="rfbD"
                     /locus_tag="PWS82_001319"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL0906463.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7032..9065
                     /locus_tag="PWS82_001320"
     CDS             7032..9065
                     /locus_tag="PWS82_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906464.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9109..10389
                     /locus_tag="PWS82_001321"
     CDS             9109..10389
                     /locus_tag="PWS82_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL0906465.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10599..11405
                     /locus_tag="PWS82_001322"
     CDS             10599..11405
                     /locus_tag="PWS82_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906466.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11417..12637
                     /locus_tag="PWS82_001323"
     CDS             11417..12637
                     /locus_tag="PWS82_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906467.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12627..14213
                     /locus_tag="PWS82_001324"
     CDS             12627..14213
                     /locus_tag="PWS82_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0906468.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14252..16390
                     /locus_tag="PWS82_001325"
     CDS             14252..16390
                     /locus_tag="PWS82_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0906469.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16758..17741)
                     /locus_tag="PWS82_001326"
     CDS             complement(16758..17741)
                     /locus_tag="PWS82_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL0906470.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18119..19510
                     /locus_tag="PWS82_001327"
     CDS             18119..19510
                     /locus_tag="PWS82_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0906471.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19526..20566
                     /locus_tag="PWS82_001328"
     CDS             19526..20566
                     /locus_tag="PWS82_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0906472.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20585..21670
                     /locus_tag="PWS82_001329"
     CDS             20585..21670
                     /locus_tag="PWS82_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL0906473.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21703..>21989
                     /locus_tag="PWS82_001330"
     CDS             21703..>21989
                     /locus_tag="PWS82_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0906474.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGA"
BASE COUNT         7126 a         3577 c         4501 g         6785 t
ORIGIN      
        1 aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc
       61 agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc
      121 ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat tcttattccg
      181 taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt
      241 cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt
      301 gattgcttct cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc
      361 ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt
      421 cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc
      481 attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt
      541 gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa
      601 tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat
      661 ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct
      721 gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa
      781 ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg
      841 ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac
      901 agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt
      961 gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat
     1021 tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata
     1081 tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta
     1141 gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg
     1201 taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca
     1261 aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa
     1321 attccggatt tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca
     1381 ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat
     1441 tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa
     1501 ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt
     1561 tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc
     1621 ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg
     1681 ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg
     1741 acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag
     1801 ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga
     1861 cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat
     1921 attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt
     1981 aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag
     2041 gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc
     2101 tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct
     2161 cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt
     2221 aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt
     2281 ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg
     2341 cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct
     2401 aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt
     2461 ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga
     2521 tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc
     2581 agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt
     2641 attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac
     2701 tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta
     2761 cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct cagctttcag
     2821 cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa
     2881 ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga
     2941 tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc
     3001 aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc
     3061 cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc
     3121 atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa
     3181 gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga
     3241 tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg
     3301 ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg
     3361 aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga
     3421 tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac
     3481 aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga
     3541 tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa
     3601 aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt
     3661 ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac
     3721 cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag
     3781 tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag
     3841 atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa
     3901 tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg
     3961 atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag
     4021 aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg
     4081 aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg
     4141 atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat
     4201 ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa
     4261 caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg
     4321 gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg
     4381 gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat
     4441 acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc
     4501 ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt
     4561 caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa
     4621 ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta
     4681 gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa
     4741 cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca
     4801 ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc agatgggggc
     4861 atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt
     4921 atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt
     4981 gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc
     5041 ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa
     5101 gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga
     5161 cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac
     5221 cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat
     5281 tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta
     5341 gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc
     5401 gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact
     5461 gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg
     5521 ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac
     5581 aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta
     5641 agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg
     5701 aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat
     5761 ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg
     5821 atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt
     5881 tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac
     5941 ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa
     6001 aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat
     6061 ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa
     6121 taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg
     6181 tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa
     6241 agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga
     6301 tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga
     6361 attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt
     6421 gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt
     6481 agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt
     6541 aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt
     6601 tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga
     6661 caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga
     6721 atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga
     6781 aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt
     6841 tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat
     6901 gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc
     6961 gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt
     7021 tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga
     7081 atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg
     7141 ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg
     7201 ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc
     7261 gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag
     7321 aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt
     7381 ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt
     7441 tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct
     7501 tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg
     7561 gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg
     7621 atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg
     7681 ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta
     7741 ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg
     7801 ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga
     7861 ttggaggggc agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag
     7921 acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa
     7981 agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata
     8041 tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc
     8101 tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta
     8161 ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc
     8221 tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt
     8281 ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc
     8341 ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta
     8401 cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg
     8461 gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg
     8521 tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga
     8581 tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag
     8641 tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca
     8701 atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat
     8761 acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa
     8821 agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa
     8881 aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg
     8941 aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag
     9001 aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac
     9061 agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt
     9121 caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg
     9181 acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat
     9241 aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat
     9301 aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt
     9361 tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt
     9421 attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat
     9481 ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa
     9541 gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat
     9601 acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt
     9661 tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta
     9721 ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg
     9781 gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt
     9841 acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg
     9901 tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa
     9961 caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac
    10021 cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg gattgctgct
    10081 ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag
    10141 atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg
    10201 acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat
    10261 gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact
    10321 tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc
    10381 tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt
    10441 ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat
    10501 agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact
    10561 tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat
    10621 ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca
    10681 aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta
    10741 ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa
    10801 aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg
    10861 catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat
    10921 tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct
    10981 tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt
    11041 tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga
    11101 cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt
    11161 tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa
    11221 gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa
    11281 gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag
    11341 taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc
    11401 tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca caaaatctta
    11461 taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc
    11521 ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat
    11581 gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg
    11641 agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga
    11701 actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat
    11761 ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc
    11821 tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg
    11881 tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt
    11941 ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa
    12001 ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga
    12061 tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac
    12121 gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa
    12181 ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga
    12241 taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac
    12301 ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt
    12361 cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa
    12421 aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata
    12481 ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa
    12541 gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca
    12601 taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac
    12661 aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg
    12721 gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca
    12781 ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt
    12841 atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg
    12901 tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca
    12961 gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt
    13021 ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg
    13081 aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat
    13141 tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa
    13201 cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag
    13261 tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc
    13321 cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa
    13381 caatccaaca aataccattc aataatttca caggggaaaa tgagacacta agtttccccg
    13441 tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat
    13501 tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa
    13561 cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata
    13621 tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa
    13681 caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg
    13741 agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag
    13801 accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga
    13861 caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag
    13921 aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca
    13981 tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc
    14041 aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc
    14101 tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat
    14161 tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga
    14221 ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat
    14281 cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga
    14341 tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc
    14401 aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag
    14461 cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac
    14521 agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg
    14581 cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag
    14641 gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat
    14701 cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga
    14761 agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga
    14821 aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga
    14881 actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta
    14941 tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct
    15001 ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga
    15061 tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga
    15121 actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc
    15181 gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata tctctcactt
    15241 aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg
    15301 ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta
    15361 ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg
    15421 cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag
    15481 aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta
    15541 tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga
    15601 tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat
    15661 tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca
    15721 tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat
    15781 taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac
    15841 agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat
    15901 cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt
    15961 attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg
    16021 ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat
    16081 ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt
    16141 caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca
    16201 tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa
    16261 acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc
    16321 gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag
    16381 accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt
    16441 ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg
    16501 ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt
    16561 tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt
    16621 taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc
    16681 gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata
    16741 ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc
    16801 actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta
    16861 gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa
    16921 tctaataccc accataaata gccatatctc atattttgta agtcttctaa agcactggtt
    16981 ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc
    17041 cctttattta ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca
    17101 tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta
    17161 tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt
    17221 atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc
    17281 tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt
    17341 ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag
    17401 acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg
    17461 attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct
    17521 aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa
    17581 gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc
    17641 cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt
    17701 tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta cctcactttc
    17761 ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac
    17821 aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg
    17881 ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca
    17941 ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt
    18001 gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt
    18061 tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga gtgtatatat
    18121 gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat
    18181 tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt
    18241 acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt
    18301 gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt
    18361 cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg
    18421 ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt
    18481 aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt
    18541 cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa
    18601 aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa
    18661 tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat
    18721 caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga
    18781 aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc
    18841 atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt
    18901 ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa
    18961 agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga
    19021 attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt
    19081 tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg
    19141 ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc
    19201 acgtcctgaa cgttctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt
    19261 acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc
    19321 tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct
    19381 tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg
    19441 acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat
    19501 ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt
    19561 atacaatgca gaagcaacta ttggtcgaac gattgatatt ctaaaagaat tggattatga
    19621 agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca
    19681 agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg
    19741 attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc
    19801 agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc
    19861 aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt
    19921 ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa
    19981 agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg
    20041 tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg
    20101 aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga
    20161 atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt
    20221 cgaaacattt aaagaattct attcattagc aaatactgta acagacgata ttaataaaga
    20281 cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac
    20341 ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt
    20401 gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt
    20461 gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga
    20521 agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa
    20581 tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt
    20641 catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta
    20701 tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag gaggccgtat
    20761 ttttgttgta cctaatccta agaaaaatat ttttaaatat ttaatagaag tgaataagtt
    20821 actaaaaaaa gaatcttttt ctatagttca caatcaggtt tattttggcg ggggaattaa
    20881 tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc atgcaactga
    20941 agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt
    21001 gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa gtctgtttca
    21061 aaaccatccc tttgagatag ttcataatgg tattgattta gagctttata gtaaaaattc
    21121 agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt ttgttgtggg
    21181 aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga tttttttaga
    21241 aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt cacttagaaa
    21301 taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat ttttaggaga
    21361 aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc cttctcttta
    21421 tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat tagtcttagc
    21481 agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat cattagatga
    21541 aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata aaataacaca
    21601 agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta tatatagtag
    21661 agatcattaa aaatgttcaa gatattttag gagtgattat ttttgaaaat atgttatttg
    21721 atattggcat acaatgatat tggcaactta aagagattgg tcaatcgatt gaacgaaaat
    21781 gcagttttta ttattcatat agataaaaaa actgatatag aaccatttat tgaagcattt
    21841 aaatcatatg ataatgttga atttttatgt aataaaaacc gtgttaaaat ttattggggt
    21901 ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga gagcattaca aaatgagaaa
    21961 tatttaaaat atgtattatt atctggtgc
//
LOCUS       DANJJW010000038        21988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000038 DANJJW010000000
VERSION     DANJJW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..395
                     /locus_tag="PWS82_001331"
     CDS             <1..395
                     /locus_tag="PWS82_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906475.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="PWS82_001332"
     CDS             398..1147
                     /locus_tag="PWS82_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906476.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="PWS82_001333"
     CDS             1159..1428
                     /locus_tag="PWS82_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906477.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="PWS82_001334"
     CDS             1433..1597
                     /locus_tag="PWS82_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906478.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="PWS82_001335"
     CDS             1590..1892
                     /locus_tag="PWS82_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906479.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="PWS82_001336"
     CDS             1889..2050
                     /locus_tag="PWS82_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0906480.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="PWS82_001337"
     CDS             2047..2352
                     /locus_tag="PWS82_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906481.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="PWS82_001338"
     CDS             2352..2708
                     /locus_tag="PWS82_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL0906482.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="PWS82_001339"
     CDS             2695..2913
                     /locus_tag="PWS82_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906483.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="PWS82_001340"
     CDS             2910..3329
                     /locus_tag="PWS82_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL0906484.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="PWS82_001341"
     CDS             3326..3883
                     /locus_tag="PWS82_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL0906485.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="PWS82_001342"
     CDS             3880..4176
                     /locus_tag="PWS82_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906486.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="PWS82_001343"
     CDS             4253..4666
                     /locus_tag="PWS82_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL0906487.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="PWS82_001344"
     CDS             5012..5386
                     /locus_tag="PWS82_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906488.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="PWS82_001345"
     CDS             5423..5590
                     /locus_tag="PWS82_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906489.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="PWS82_001346"
     CDS             5616..5960
                     /locus_tag="PWS82_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0906490.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="PWS82_001347"
     CDS             5965..6246
                     /locus_tag="PWS82_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906491.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="PWS82_001348"
     CDS             6349..6663
                     /locus_tag="PWS82_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL0906492.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="PWS82_001349"
     CDS             6641..8335
                     /locus_tag="PWS82_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0906493.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="PWS82_001350"
     CDS             8355..9533
                     /locus_tag="PWS82_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0906494.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="PWS82_001351"
     CDS             9496..10182
                     /locus_tag="PWS82_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0906495.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="PWS82_001352"
     CDS             10182..11342
                     /locus_tag="PWS82_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0906496.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="PWS82_001353"
     CDS             11352..12227
                     /locus_tag="PWS82_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906497.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="PWS82_001354"
     CDS             12224..12535
                     /locus_tag="PWS82_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906498.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="PWS82_001355"
     CDS             12525..12878
                     /locus_tag="PWS82_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0906499.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="PWS82_001356"
     CDS             12868..13269
                     /locus_tag="PWS82_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906500.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="PWS82_001357"
     CDS             13262..13666
                     /locus_tag="PWS82_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906501.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="PWS82_001358"
     CDS             13678..14286
                     /locus_tag="PWS82_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0906502.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="PWS82_001359"
     CDS             14306..14668
                     /locus_tag="PWS82_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906503.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="PWS82_001360"
     CDS             14671..14853
                     /locus_tag="PWS82_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906504.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="PWS82_001361"
     CDS             14870..18289
                     /locus_tag="PWS82_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0906505.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="PWS82_001362"
     CDS             18340..19077
                     /locus_tag="PWS82_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0906506.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="PWS82_001363"
     CDS             19087..21378
                     /locus_tag="PWS82_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0906507.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAQRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="PWS82_001364"
     CDS             21402..>21988
                     /locus_tag="PWS82_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906508.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7934 a         3244 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacaa
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DANJJW010000039        21732 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000039 DANJJW010000000
VERSION     DANJJW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(152..550)
                     /locus_tag="PWS82_001365"
     CDS             complement(152..550)
                     /locus_tag="PWS82_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL0906509.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="PWS82_001366"
     CDS             complement(547..1320)
                     /locus_tag="PWS82_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0906510.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="PWS82_001367"
     CDS             complement(1317..2000)
                     /locus_tag="PWS82_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0906511.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="PWS82_001368"
     CDS             complement(2167..3054)
                     /locus_tag="PWS82_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906512.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="PWS82_001369"
     CDS             complement(3051..3920)
                     /locus_tag="PWS82_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906513.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="PWS82_001370"
     CDS             complement(4057..5343)
                     /locus_tag="PWS82_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0906514.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="PWS82_001371"
     CDS             5616..6194
                     /locus_tag="PWS82_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0906515.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="PWS82_001372"
     CDS             6194..7123
                     /locus_tag="PWS82_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0906516.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="PWS82_001373"
     CDS             7120..8856
                     /locus_tag="PWS82_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0906517.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWS82_001374"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWS82_001374"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL0906518.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="PWS82_001375"
     CDS             complement(9752..10261)
                     /locus_tag="PWS82_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL0906519.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWS82_001376"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWS82_001376"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL0906520.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="PWS82_001377"
     CDS             complement(11562..12653)
                     /locus_tag="PWS82_001377"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL0906521.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWS82_001378"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWS82_001378"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL0906522.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWS82_001379"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWS82_001379"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL0906523.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWS82_001380"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWS82_001380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL0906524.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="PWS82_001381"
     CDS             complement(16616..17590)
                     /locus_tag="PWS82_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL0906525.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="PWS82_001382"
     CDS             complement(17571..19454)
                     /locus_tag="PWS82_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL0906526.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="PWS82_001383"
     CDS             complement(19472..21697)
                     /locus_tag="PWS82_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL0906527.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6198 a         4790 c         3859 g         6885 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac tt
//
LOCUS       DANJJW010000040        21618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000040 DANJJW010000000
VERSION     DANJJW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            68..751
                     /locus_tag="PWS82_001384"
     CDS             68..751
                     /locus_tag="PWS82_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL0906528.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="PWS82_001385"
     CDS             753..1589
                     /locus_tag="PWS82_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0906529.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="PWS82_001386"
     CDS             1600..3354
                     /locus_tag="PWS82_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906530.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="PWS82_001387"
     CDS             complement(3630..3971)
                     /locus_tag="PWS82_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0906531.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="PWS82_001388"
     CDS             complement(4039..6210)
                     /locus_tag="PWS82_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0906532.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="PWS82_001389"
     CDS             6379..7278
                     /locus_tag="PWS82_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0906533.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="PWS82_001390"
     CDS             complement(7349..8206)
                     /locus_tag="PWS82_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0906534.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="PWS82_001391"
     CDS             complement(8191..10893)
                     /locus_tag="PWS82_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL0906535.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="PWS82_001392"
     CDS             complement(10906..12195)
                     /locus_tag="PWS82_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL0906536.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="PWS82_001393"
     CDS             12345..13391
                     /locus_tag="PWS82_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906537.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="PWS82_001394"
     CDS             13393..15546
                     /locus_tag="PWS82_001394"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL0906538.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="PWS82_001395"
     CDS             complement(16019..17470)
                     /locus_tag="PWS82_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906539.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="PWS82_001396"
     CDS             complement(17467..19191)
                     /locus_tag="PWS82_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0906540.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..19798)
                     /locus_tag="PWS82_001397"
     CDS             complement(19184..19798)
                     /locus_tag="PWS82_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0906541.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(20143..21600)
                     /locus_tag="PWS82_001398"
     CDS             complement(20143..21600)
                     /locus_tag="PWS82_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0906542.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT         6116 a         4456 c         3855 g         7191 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccctca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    19501 gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta ccagataaaa
    19561 ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc cagcctcttt
    19621 ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg actgttttcc
    19681 atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag aaatacaagg
    19741 ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc cctaacatat
    19801 cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt tgggcaatca
    19861 gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata ttttgtcgtt
    19921 ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt ttgtgaaata
    19981 gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac atttttacat
    20041 atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga acaagaggtt
    20101 gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt gtcggaactc
    20161 atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt ccaaagcgtc
    20221 tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc catctaaata
    20281 ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag tgttgaagct
    20341 gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt tggcaatcat
    20401 gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat tccaagcaga
    20461 catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg aaccatcgac
    20521 tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt ctggatcact
    20581 gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat ttgatacgac
    20641 aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg cttttgagac
    20701 aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg gtccttgtgt
    20761 ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc tcgttgcagc
    20821 gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca gtccgatata
    20881 cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat caatagcaaa
    20941 tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc cttgtccaat
    21001 ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt tttctgcatc
    21061 agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag ctttgttaaa
    21121 agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata attttccatt
    21181 gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt cagcgtactt
    21241 aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa catagttgtt
    21301 cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt tcttttcgta
    21361 atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt cgattcgttt
    21421 gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt gatacatctg
    21481 caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc cgcatgctgc
    21541 taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc ataacttcat
    21601 ttttcttcct cctaattt
//
LOCUS       DANJJW010000041        21515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000041 DANJJW010000000
VERSION     DANJJW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            195..896
                     /locus_tag="PWS82_001399"
     CDS             195..896
                     /locus_tag="PWS82_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0906543.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1505..2521)
                     /gene="tsaD"
                     /locus_tag="PWS82_001400"
     CDS             complement(1505..2521)
                     /gene="tsaD"
                     /locus_tag="PWS82_001400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL0906544.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2518..2982)
                     /gene="rimI"
                     /locus_tag="PWS82_001401"
     CDS             complement(2518..2982)
                     /gene="rimI"
                     /locus_tag="PWS82_001401"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0906545.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2989..3531)
                     /gene="rimI"
                     /locus_tag="PWS82_001402"
     CDS             complement(2989..3531)
                     /gene="rimI"
                     /locus_tag="PWS82_001402"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0906546.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3515..4237)
                     /gene="tsaB"
                     /locus_tag="PWS82_001403"
     CDS             complement(3515..4237)
                     /gene="tsaB"
                     /locus_tag="PWS82_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL0906547.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4428..5408)
                     /locus_tag="PWS82_001404"
     CDS             complement(4428..5408)
                     /locus_tag="PWS82_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906548.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5432..6916)
                     /gene="galT"
                     /locus_tag="PWS82_001405"
     CDS             complement(5432..6916)
                     /gene="galT"
                     /locus_tag="PWS82_001405"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL0906549.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6928..7917)
                     /gene="galE"
                     /locus_tag="PWS82_001406"
     CDS             complement(6928..7917)
                     /gene="galE"
                     /locus_tag="PWS82_001406"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL0906550.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8165..8332
                     /locus_tag="PWS82_001407"
     CDS             8165..8332
                     /locus_tag="PWS82_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL0906551.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8394..10205)
                     /locus_tag="PWS82_001408"
     CDS             complement(8394..10205)
                     /locus_tag="PWS82_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL0906552.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10202..10567)
                     /locus_tag="PWS82_001409"
     CDS             complement(10202..10567)
                     /locus_tag="PWS82_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL0906553.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10730..11125)
                     /locus_tag="PWS82_001410"
     CDS             complement(10730..11125)
                     /locus_tag="PWS82_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0906554.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11143..12105)
                     /locus_tag="PWS82_001411"
     CDS             complement(11143..12105)
                     /locus_tag="PWS82_001411"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL0906555.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12105..12317)
                     /locus_tag="PWS82_001412"
     CDS             complement(12105..12317)
                     /locus_tag="PWS82_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL0906556.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12338..13036)
                     /locus_tag="PWS82_001413"
     CDS             complement(12338..13036)
                     /locus_tag="PWS82_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL0906557.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13056..13598)
                     /locus_tag="PWS82_001414"
     CDS             complement(13056..13598)
                     /locus_tag="PWS82_001414"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL0906558.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13730..14734)
                     /locus_tag="PWS82_001415"
     CDS             complement(13730..14734)
                     /locus_tag="PWS82_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0906559.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14731..15720)
                     /locus_tag="PWS82_001416"
     CDS             complement(14731..15720)
                     /locus_tag="PWS82_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0906560.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15717..16523)
                     /locus_tag="PWS82_001417"
     CDS             complement(15717..16523)
                     /locus_tag="PWS82_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906561.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16689..17645
                     /locus_tag="PWS82_001418"
     CDS             16689..17645
                     /locus_tag="PWS82_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0906562.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17722..18240)
                     /locus_tag="PWS82_001419"
     CDS             complement(17722..18240)
                     /locus_tag="PWS82_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0906563.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18328..18477)
                     /gene="rpmG"
                     /locus_tag="PWS82_001420"
     CDS             complement(18328..18477)
                     /gene="rpmG"
                     /locus_tag="PWS82_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0906564.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18705..19151
                     /locus_tag="PWS82_001421"
     CDS             18705..19151
                     /locus_tag="PWS82_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL0906565.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19346..21241)
                     /gene="asnB"
                     /locus_tag="PWS82_001422"
     CDS             complement(19346..21241)
                     /gene="asnB"
                     /locus_tag="PWS82_001422"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL0906566.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6476 a         4562 c         3703 g         6774 t
ORIGIN      
        1 ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca ataccattgt
       61 atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt tctaatgttg
      121 gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag
      181 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
      241 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      301 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      361 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      421 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      481 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      541 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      601 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      661 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      721 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      781 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg ttagattatg
      841 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      901 aacaaaaata agcagtgacc ctgataaata tcagggtcac tgcttatttt cctccctaaa
      961 acttctgaaa gatccggcag ttttagatca ctggacaaga ctcaaagatt ttctatgtgt
     1021 gtttcagtta tgtaatattc actgtttgca aaaaatactg ttagattaac tagatgaagc
     1081 gtcttgtcct catccgatct agattattcg atattcctcg acaataaacg attactcagt
     1141 gagggcaatc agaggaacgg ataatgataa ataagctgta aaaaatagca aaataacaat
     1201 aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa cgtgtcacag ccctatttcg
     1261 attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca taaacatgag
     1321 gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct tacgaactct
     1381 tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag gtcaaaaaca
     1441 tgagaagccg tttttcctgg gctgttctta ttacttgatg cagggcagct ttttcacaac
     1501 ctctttattc agataaaacc aagcttggat cagcattcaa ctgatagcta gcaaaatttc
     1561 ttttttgcat ttccacatgt gctgctgctc caatcattgc agcattatct ccacaaagtc
     1621 gaagaggggg aatcactagt tctacctcag ggagtttagc tgccaaagca tcctgcaatc
     1681 cttcacgcaa cccttgattg gccgctacac ctccagctat aatcagttgc ttgactggat
     1741 aattttgaca agctcgaagt gttttagtaa tcaatacatc gatcacactt gcttgaaagc
     1801 tagctgctaa atcatttttg tctagatctt ctccacgctg ttgtgcatta tgcaccaaat
     1861 tgataaatgc actttttaat ccgctaaaac tgaaatcata attatcttca tggatcatcg
     1921 ctcgcggaaa atggtaatta tcttttccct gatgagctag ctgatcgatt tcttttccgc
     1981 ttgggtaaga aagacctaat actcttccaa ctttgtcata agcttctcct gctgcgtcat
     2041 ctcttgtctc cccaatgatt tcatatgatc catcttcttg catatagact aattctgtat
     2101 gtcctccgct aacaagaagt gccatcaatg gaaactggaa tggtttaacc agacgggctg
     2161 catagatatg ccctgccata tggtttacag gaataagcgg caattggtgt gcccaagcaa
     2221 aagccttagc tgcagaaata ccaatcagca acgagccgac aagtcctggt ccataagtga
     2281 cagcgactgc cgataaatct tctgccgaca caccagcttc taccagtgca tcttccaagc
     2341 ataaggtgat ttgttctacg tgatggcggc tagctacttc aggtactacg ccaccaaaac
     2401 gtttatgact gtttatttga gaagcaacga tgttagataa gatctctgtt ccatttctaa
     2461 cgaccgctac actcgtttca tcacaacttg attcaatcgc cagaatcaat tcttcattca
     2521 tttcatcgtc tccgttttta actttctgct cataatgatc gcatcttccg tcggattgcg
     2581 ataatatgat ttccgtttac ctaatatacg aaacttttct gattcataaa gttttcttgc
     2641 tgcttcatta gatagacgta cttctaaaaa aacatgcagc gaatctttta tttgttccgt
     2701 aatcagtaat tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc
     2761 aatatttgtg atctcgactt catctattac tttcgagtat ccaataaagc cacagatctg
     2821 gttatcttca gctaaaagca gataatcaga gtgaggctgt tggatgtctg ccaaaaactg
     2881 cattttcgtc caaggtgaac catgtggata agcattttcg ctaatcatcc atacgcattc
     2941 tgccaagtac gcttcatcaa aatctttttt tctgcaaatc aactaatctc actcattttc
     3001 taaaagtttt atcatatcca acgcatcttc ttttgtttca gtgtaatatc tttttttgat
     3061 cgttgttgct tgaaacccca atttccgata aagtctttgt gcatcttgat tactcatacg
     3121 cacttctaat gacatcgtct cacagcgatt cattatagca aagttctcga tttcgtcaat
     3181 aagcagcgaa ccgattcgtt tacgctgata tgccggtcga acggcaatat tcgtgatatg
     3241 gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc attcttccat tatcaaagat
     3301 acctagatat agatgaggaa gcggtgactt caattctgat ataaaggcac tttttgtcca
     3361 tggcaattcg ccattataga cttctcgttc gacatccagc agctctttga tatcttctgt
     3421 agtcaattct cttagtacgt aacgacgatc gatttgttcg actgtttttt ctgtataaat
     3481 cattttatct tgaaaaaagc ttctggcata cgctctaaat tttttcaaca taactttcgt
     3541 ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc tactcgtttt aaatagcgag
     3601 gaagaaaagc ttgtttctct gcaactggtt caactaaagc acctaattga gccacagtta
     3661 caccacttgg aatatcccat gctggaactg tattaatcat cgcactaggc aatatttcct
     3721 ttatttcaga gaagaatttg tgacaatcac tcccgacgaa ataaatagct tcattcagct
     3781 tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt acagtttcta
     3841 atctattttc tttccagcgg tatgccccag cgtatacatt ttttcttcga gcatcaaaca
     3901 tgggcacaat aatattatcc acaccgatgc aattagccgc gatggttttc agactagata
     3961 ctcctactag ctctttatct aatgtataag caatcgtttt tgctgtcgtt acacctatac
     4021 gaagccctgt ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg tcttttggac
     4081 ttatttgaag atctgcaaac aattggtcga ttgcaggcat cagtgttaca ctgtggttgc
     4141 gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca
     4201 aagtttggtt tgcagtatct attcctaatg taatcatata tttgatcctt tctgctatga
     4261 cattctcgcc ttccattgta gcatactttt ttattctgag gatgaaaata ccaaaggcaa
     4321 aactagtaca ataatacatg attatctgct ggcgggcaaa ttttgcccct tcctgaaaca
     4381 caaaaaagct gcggtatcac tccattttcc cacagctttt ccatctttta tttcgttgat
     4441 tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc tttggaatta
     4501 cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata aacttttaca
     4561 gtagaaagcg agggcgttac atatttcgct acactgacat cattgaagcc catcacagaa
     4621 atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc aatcgctaat
     4681 gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa atattgcttc
     4741 actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc ttcgtcgtag
     4801 cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag ccgctgatgg
     4861 tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt atgggagaca
     4921 aagtgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga gtcgattcct
     4981 tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt catttctaat
     5041 aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgccggaag atcactaatg
     5101 gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt ttcgattcct
     5161 aggcgaatag ataaataata aagatcttca agctcttccg tatcgtcgta ccactggatc
     5221 aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata atttaagcgt
     5281 tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc aggatcatag
     5341 ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat gtctttaatt
     5401 gttgccattt gtctctcctc cttttttacg tttataaaat cgcagtaaat cgttcaaacg
     5461 ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa
     5521 cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt
     5581 ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt tcttctccta
     5641 ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa acagcaagcc
     5701 ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct ttatgtggat
     5761 gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca aggtccaact
     5821 caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg
     5881 ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccatttt tccaatactg
     5941 agttagctgc ttcaataagc aagtttttct ctgttgattg caaacggatc acagacatcg
     6001 gccatttcac gactcctgcg atgacttgcg gataatcttc tagtttgata agtttttcta
     6061 tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca tgagaaagaa
     6121 tcgatcctcc aacaattggt aagtcagcat tcgaaccaac aaagtaatga ggcaagactt
     6181 cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt
     6241 ctgataaaat gatcgaatgt tcattgtaat aagcataagg ggaatattgg aatccccagc
     6301 tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga tagttcaagc
     6361 gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct acttttaccg
     6421 cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa ttgattgtga
     6481 tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg atggctcttg
     6541 ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct tcctctgggc
     6601 ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt gtgagaaaat
     6661 ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga acaccatttt
     6721 ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta actggtctta
     6781 cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc tgtaaatata
     6841 agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg
     6901 tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat ggctgacatg
     6961 ccaatcccat gctgttttga taatcgcttt gatatctgtg tattcaggtt tccagcccag
     7021 aatctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag ctcttcttgg
     7081 tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta gcatttcctt
     7141 gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc cttctttcaa
     7201 gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat aatctctgat
     7261 acatgttcca tctggtgtat cataatcgtc tccataaaca gccaaggctt ttctttgacc
     7321 aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat cttctccgat
     7381 agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat agcgcatgcc
     7441 atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt caccatacgg
     7501 atttttagga ttagctggtg tatcttctgt gattggagac tctttcggct ctccataagt
     7561 tgctgcagta gaagagaata cgattttatt cacgttgaac tcgtgcatga cttcaagtaa
     7621 gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga cagattctcc
     7681 tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt caaatacgga
     7741 acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc
     7801 ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct tttgaactag
     7861 ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa ttgtcataaa
     7921 aaacactcct ttgattagta aaattgtgtt tgttgattct atataattta aaaaacgaag
     7981 tttactttta tggtgaaaac aaacaaagta aaatttcgta aattcatatg gtaaatttat
     8041 aataaaacat ttagtaaaca ttttcaaggg ttttttgcaa aggtttacaa acaaacgtaa
     8101 aactagcctt tttttctctt ttgttccata atagatataa caaataataa atgaggtgga
     8161 ttcaatgaaa aaatttgtat ctggaatatt agtaggaagt ctggcaacag ccgctgccgt
     8221 tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc
     8281 aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt
     8341 taaaaatacg catgaagact ttctaaaaaa cagataagtc atcatgcgta tttttatttg
     8401 tcagtcaata taagttgtgc tattttatct gcctgtctca gattccatgg atctgtataa
     8461 ggtctagcta ctgctgcagt tagccaatcc acaccttcaa caggtattcc atagcaataa
     8521 aagtcagact ggatttcccc tgaagcatca atcacgcgat tcgtacctcg ctcaacatct
     8581 atagcttctg aatgataact tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg
     8641 ttttccttca aattttttat gacaggatta cttgaacgag aaaagcttgt ttgtggcaat
     8701 cgagcttcaa tcaaatagcg actgctgatt tctttatctt gtagagaaat aacatagcgg
     8761 ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc
     8821 agtgctgcta attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga
     8881 tgagtaaacc atccccatag tatttctttg tattccttag cagaaaaaac gtgccattca
     8941 atagcctgcc ggattggatc acgccagtct tttaatgcat caaatgttga agcaaatgct
     9001 cctgtgcaat tgcctttttc cgcttctttt atctgccctt caataaatat tcggctagct
     9061 tctgaaaagg gctcattctg ataggcaaga ggagtttcta aatacgacca atcccatcta
     9121 taagggatta gtgctggatg ctttccgaga cactcctctg aagagaggga gagaaaatca
     9181 tgctcaaaat ttttctttgg aatcggcaag tgatattctt cgatgatttt cttgtaataa
     9241 aacaactcaa cgtctttttt cagatagtca aaaaatgttt cgccagtcaa aaatcccgac
     9301 tgatgccatt gctccaggtt ttcttttgtt agtaaccgtg gccatgccat tgctccgcct
     9361 tgcttttgat ttcttcctcg agggtaataa gggagccccc ttcttgatcc actgtataca
     9421 atcggttcct tgcctgatgg atgatagacc agttttccct tttcgttctc gaagcgccct
     9481 cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag acctcttaaa
     9541 aagacatttt ctttaggcgg tatcttcgaa agatctacat ctgcaggatt agaaggaagc
     9601 tgatacagta aaacattttt ctttgcgtag tctacccaca ctctttcttc ttctgtctca
     9661 tcattttccc agtgaccgct tgctaaaaca agctgatcga tagaatagct ttgctgatct
     9721 gtctgtaaaa gataaccatc ttttcttttg cttacttttt ttactaaaga ataaacaaac
     9781 tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa accagcgttg atagagtccg
     9841 tataaacatc gagtagattg ctgatctttt tccagacgat tgatttcttc tataattagt
     9901 tgcttatttt tggttaccgt ctgttcgata taatctgtag caggtccttt actccattga
     9961 aacaagttgg gtccctgtgt taccaatcct ccactggaca atgtttcatc tgtaaaaagc
    10021 gtcacttgtt gagaaacaga attcattaat aactccggtg gctgattcaa ccgccaaact
    10081 cgacctccag gaccattggg atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta
    10141 ttatggagaa tcctttctgc tacacttaat cctctaggtc cggcaccaat gatccctatt
    10201 ttcatttgca tgcctccatt tcgataaatt ttttcaataa aacagcatgg ttctgatgtt
    10261 cattttttgc accataaaca aaagtaatga ctggatgttc tttcataagt tgactgagtt
    10321 taaaaatagc ttgcttcttt tcttcttcga aaatcaattc ttgctggtat ttctttgcaa
    10381 atgaagcaaa tcgttctggt acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa
    10441 tttctttcaa ccacagatct aactgcgctt tttcttttga aattcctcgt ggccagaatc
    10501 tatccacgag tattctatag ccatcctctt tttcagcatc aaggtaaaca cgttttaatt
    10561 tgatcatagt tgtttccttc tttctacctt ttcgcttagc ttatatatgt agataatagc
    10621 acaaaataaa aaaaatgaac ggcacaacta tccattgcgc cgttcatgtg atcttggtca
    10681 aaatctaatg attgattcgg tttttaaagc tgtcatacaa tcatctgttt tatacttttt
    10741 tacttttgat ttttccagac catccgtcat atccaccctt aagtaaataa atctcttgat
    10801 aacctgcttt acgtaattta tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa
    10861 tatagacagg ctggtctttt cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga
    10921 tgtttcttgc acctaaaata tgcccagcat caaacacatc tttttcgcga acatcgatta
    10981 cttgtgcttt tctcattcct tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt
    11041 tggccattac tcgtagatag acttcccaaa ggatgattgc aagcacaatc aatagcaaaa
    11101 tgatattaat tacccataaa accatgtaat gcctccgttt ttttaagcaa atttcaaggc
    11161 taatgaggca gcaccaatga caccagcttc attaccaagc tctgctaatt tgatttttgt
    11221 tgattctctt acttgcggga aagtaaattc tttaaaatat ttctctacac ggctacgtaa
    11281 aaattctcct gctgccgata ctcctccacc taacacgata cttgacggat ttaatgtatt
    11341 ccccaagttt ccgcaagcta aacctaagta gaagcatacg cgatcaacaa ccatcaatgc
    11401 aaacggatcg tcttcttgag ccatttcaaa gatatcttta cttgttactt cttctccgtt
    11461 gtctagcatt gctttcagtt ttgaatcccc tgcgtattct tctgccaaga aacgacctag
    11521 gcgtaccaca cctgtagcgc tagatacagt ttctaaacat ccgcgtttac cgcaagtaca
    11581 ttcgaaccct tcaggatcca cagtgatatg tccgatttct cctccagctc cgctcacgcc
    11641 gtgaatcaaa tttccgccag caacgatacc tccgccaaca cctgttccta gagtaacgaa
    11701 tacaacgtct ggatcgtttt ctccagcacc tttccatcgt tcacctaaag cagcaacatt
    11761 cgcgtcatta tcgatagcga atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa
    11821 ttgaacttct gtccagttta agttatacgc accaataacc gttccagctt cgctgtccac
    11881 gcttcctggg gtacccatgc cgattccgat aaagtcttcg gctttcatat tatataaatt
    11941 caaacgatga ttgattgatt caatgatttc aggaacgata tgcttaccat catccaaaat
    12001 attggtatcg atgctccatt tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt
    12061 tgctgttgtt ccacctaaat caatcccaat aatcttcttg tccattacaa atttcctttc
    12121 tttcggcttt cttcaattcg gtgctctctt tttaatatgc gccacgctgt catgtatgta
    12181 tcatgatcaa tcaaacggct atcatacaga ttacgcaatt cgatagtcat taattcaatg
    12241 tcatagatgc gtgcacccac atagataaag atgccaaatt ttttgaacag ctgctgtaca
    12301 tcatataagc tctccatctt taagcacttc cttcatatta tacaagtaaa ccatattttt
    12361 ttaaacccag taaaatacat attacaaaaa gaaatacaaa aactagtgta gaaagaatcc
    12421 tttggtgtat cgaatagctt gtctgttgtt tcggtagacc caaaacattc cctaacacta
    12481 atccgccaat tagcccaccg acatgtcccc aaacatctac agaggtatcc agaagaccaa
    12541 aaacaaatgt caacacaata aataaaagaa aatgtctgac tagctgctgg atcgctgggt
    12601 tatgtttaaa atgaaaccct aagataaaca acgcaccgaa caaaccgaat atcgaagtac
    12661 ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta
    12721 tcccgctgaa catataaatc aagaaaaaac gccaatggcc atagattgcc tcgatttgct
    12781 gccccataaa atacagtatc agtgagttca ttacgaaatg catcagtcca aaatggatga
    12841 agattggtgt cactaaacgc caccattcat tgaagtgaac aagataagga ccatacattc
    12901 cggcataata agggatctca agtcgcggaa ataattccat catgaggaag accgctgtct
    12961 gtatccctaa aaaagcatag gtccataacg gtcgtcttaa ccatgcacgc aattttattt
    13021 gctgttgata attcatgaaa acctcccatt tcattttagt caataaacaa ttgttggatc
    13081 ttctgatcaa agctctccgg ttgccattgt tcattcaatt gttcacgaaa aacaaggcta
    13141 catgtttttt tatgaaaatt atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt
    13201 cgataaccgg ctctattgta gataatacct ggtacaatga ggagatcgat catctccggc
    13261 aaatagagct tctctgattg tggctcaaga acaccaaaac tcgaacgtac aagaacggac
    13321 tgttcatcca tttcataaaa cgccatctga cgtttcggca aagttttagg tacaactatt
    13381 ttcttttgtt ctttttctgc tcgttggata accggctgtg tatccagttc aaattcattc
    13441 gataaagtcg tccctatcac ttttgctgac ttccagtact cgctggcgaa tagctgttta
    13501 tagattcgct gttctttttc ctttttcttg ccacttttgg ccagattttt cagcttttcg
    13561 attcctttcc ggcgtaatct tactttcaat tggtccaaaa actcaccctt ccttctctca
    13621 taaaatggta taggaaaaaa agaaaaatag caatcgaact ttcaaataaa tgcataaata
    13681 gaataaaaag aagacctaga aatgattggc cttccttttt taatgaattt caactgttca
    13741 gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata tggcgcacca aaaagggcaa
    13801 tcaaaattcc tgtaggtact tccccacttg gtagaatcac tctagcaacc atatctgcac
    13861 taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa ggtccagtta ttttctttac
    13921 gtacgactag cttcgcagca tgaggagcta gtagtcctac aaaagataaa ctacctgtca
    13981 ccgctacact accgcttgcc aaagcaacag ctaacaacaa aaggattttt tgacttttag
    14041 ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct
    14101 gtgcataaag tcctcctagc ggaattaaga taaccgccca tggaagcaat gcccaaacat
    14161 aaggccagct cgcaccccag attgatcccg ctaaccatgc attcacaaat ccataatttt
    14221 ccttagataa ttggatcgtg catagcaatg ttaatgcaga aaggccggca ttaacggcaa
    14281 taccggacaa taagattcga ttcataccca aaaagccatt tcgatcatag gccgcaagat
    14341 agacacagca agcagctaaa acaccaccta gaaaggctac aactagcaat agaaacggca
    14401 gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa aaatcctgct ccggcattaa
    14461 tgcccaatat actagaatct gccaattcat ttcttgttac tccttgcaag atataaccag
    14521 aaacgccaag acaagctcct gctaataaac tgatcagtaa tcgaggaact cgaaattctc
    14581 taacaatcag cgccaaagaa gatgattctt tccctatgaa cgtggcaaaa agctgatcca
    14641 ggtaaataaa aggcgtacca atcattaaac tacctataga caggatgaca agtacaaaaa
    14701 gcaagaaata gaacgtccaa tatatttttt tcaccgttgt tccctcctta cttgtatcag
    14761 taaaaacgga acgccaatca atgcaatgac taatccaaaa ggtgtttcaa aagggggatt
    14821 gatcatcctc gctgtaaaat ccgccaacat aaccaatcca ccgccaacta aaaaagagac
    14881 aggtagaaca taacgatagt ccgttccaac cattccgcga acaactggcg gaaccaacag
    14941 cccaataaat gaaacaggtc cgactaacga aacggcaatg gctgctaaca ctgcaactag
    15001 acagaaaaca atcaaccgcg ttcgtttagg atgacttcca agagctaatg catgtgtttc
    15061 actcaaatga atcaaagata ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat
    15121 atataaagga aacaatactg ctaattgttt ccatgtgata ttggcagctc cgcctacaaa
    15181 ccaaaaagca agatcctgct gcagattaaa gaattgggta agtgcttggc tgatagcagt
    15241 aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt aaaggattca tgcccatttt
    15301 cgaaaactgc aaaaaaccaa atacgagcaa agttacagca aaagaacctg ccaatgacat
    15361 tagaaacaca gaaaaatcag aagctttagg ccaaaaagca aaactaaggg ccattgccaa
    15421 cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct aaaggatttc ttgtcacccc
    15481 ttgcatcaat gcaccgctca atgcaaaaca actccctaca agaaatgctc ccgccacacg
    15541 aggcaaacga atatttcgta tgatttggtg actttgtttt gatctatcaa aatcaaacaa
    15601 cgcctgccaa aaatctgctt tttccacagt cgctgcgcca tttgaaagag caacaaaaaa
    15661 aataatcaaa agaacaaaac agaccaaaag caggagtcta tttttcttca tagtcctcat
    15721 aattgatcct cctgctcttt tggcaacaaa tcataggtta gaaaaactgg tttatttgtt
    15781 cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt ttttcataca ctcttttgtg
    15841 atcacttctt caggtgtacc ttctgtgatc aacttaccgt ttttcattgc taataaataa
    15901 tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc
    15961 atttgattga tttcagtcaa taactgtagg atctctaatt ggtgtgccgg atctaaaaat
    16021 gttgtcggtt catccaagat caagatatct gtttcctgag caagcgccat agcgatccag
    16081 acacgctggg tttgtccacc agacagcgtt gcgatatccc gcatacgaaa tccagctaga
    16141 cctgtagcat ccaacgccca ctcaattgct tcatgatctt gcgtgtccat gccactgaac
    16201 agtttttgat aagggtatcg tccgtaagat actacttctt ctactgtcat acctgtaggg
    16261 tgttcactag tttgtgctaa taatgcaatt ttttttgcaa gttcttttga actgtatgta
    16321 gagatatcag agttattgac aatgatcttt cctgatttca caggtagtat acgggtaaaa
    16381 cttttcaata aagtagattt tccacttcca tttggcccaa tcaaactagt gattttccct
    16441 tcaggaatcg taacagacat gttttcaatg atttttttgt tttcgtagcc ggctgcaatg
    16501 tcagctgctc ttaatctcgt catttcaccc atcctttcgc tgaaaatcat tctcaattat
    16561 gacgctgttt caacatttcg tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt
    16621 gacagcgttc gaaatatcag gaataataac caatgagaaa gattctcaat tatgttagga
    16681 ggaaagaaat gaaattaaaa acaaaagtat tcacattatc tagcatattg ctgctggctg
    16741 gagtactgag cgcttgctcc ccctcttcag ataaagaaac agctacaact gccaattcga
    16801 cgacttcatc caatagtact catacagtca cagatacact tggacataaa gtagagattc
    16861 caaacgcgcc caaacggatc atcggcagtt acttagaaga ctatctgatt gcactagggg
    16921 aaaaacctat cgcccagtgg actgttggca gtggttccat tcaagattat ctgcaagatg
    16981 atttaaagga tgtcccaacg atttcttatg accttcctta tgagaaagtg ttaagctttg
    17041 agcctgattt gctgttgatt tcttcttccg ctactgtcga aggcgggaaa tacgaacaat
    17101 atagcaaaat cgcgccaact tatgttgtaa aaaacggaac agatgtcaca tgggaagaac
    17161 aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga ggcagaaaaa atcatttccg
    17221 attatcaaaa atccatcaag aaaactcgag aagaactagc agataaaatc gagaacaaga
    17281 cagctgcagt gttgtgggtg accaacaact cagcctttat ggtcagcaaa aatcggtcaa
    17341 gtggaagaat cgtatacgac gatctggaat ttggtgttcc tgatctagtg gaagaggtct
    17401 ccaaaaaagc cacttctgac tggtctgctg tttcctctga aaagctagct gaattagatg
    17461 ctgactacat catccttgtg aacagtgaca aagatgctgc catgttcaat gaaccaagct
    17521 ggaagaatct aaaagcagtg acagacaatc atgtactgca attcggacct gaatctagtt
    17581 ggctatataa cggacctatt gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa
    17641 attgatttta actaaaaaaa gcatcttcac gattagtcgt gagatgcttt ttttcttatt
    17701 aacaaactag acgcatagat tttattcttc ttttgaagca accctatttt ttactctttc
    17761 ccatttttga aaacgatggg catatagatt ttcttgatcc gttatcccct catactcctc
    17821 tataagggcg aagtctgaaa aatcaatatt cccaatatac gtatcgcctt caaacgtgct
    17881 atcgatcaat gttcgccaga tccgaccagt gtaaggaaga agttcttgaa aaatccgact
    17941 accacccgaa acatagatat cttctgagat cgtttgacta tacgacaaaa gttcatcgat
    18001 cgaatgcatt acttccgcat tttctttcgt gcgaaaattt ggttgcgttg tcaacacgat
    18061 gatatggcga taaggaagtg aaagatcccc cattccttca taggtctttc ttcccatcac
    18121 taaaattcga tccattgtat gttcgcgaaa gaaacgcata tcatttggca atcgccatgg
    18181 taacaaacca tttttgccga tcagaccatt tttatcttgt gcccacatgg aaataaacat
    18241 attatccgct ccttatgatt ttagatgttt tgagataaaa aaagtctcaa ttctttgacc
    18301 agcaaggaat tgagaacttt aaaaacatta ttttgtttca cggtgcaaag taactttgcg
    18361 ttcgcgtggg caatattttt gtttttccaa acgatcagga ttgttacgtt tgtttttgct
    18421 tgttagatag ttacgttctt tacaagaagt acactctaaa gtgatgttta cgcgcatgtt
    18481 tttccctccc atttttacaa gaatcctcta agatgactct cagacttgat tatcttacca
    18541 tgattttttc tagattgcta gtgtttttca cgattttgtt aagtaaaact acttgacgat
    18601 aaatacaacg gttttacttc tcaaacaaaa tctttttctt tttgtataaa tacccttaca
    18661 cttgtttata tagattatgt tttttaataa agggggatac taatatgcca tggaacatga
    18721 aggactttcc tgcatcaatg aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca
    18781 tcgcaaatgc attattagat gaagggtacc ctgataaccg tgcgatccca attgccattg
    18841 atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag
    18901 aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc tcgtgctgga aaactgcttg
    18961 attcagatgt gatcgtagaa tatgaggaag aacaatggat cgtgaaatct aaaggggcaa
    19021 aaaaggcaag caaccatttt gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag
    19081 cacaaaacaa agaatcaact ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa
    19141 tcgattattg aacatatgta aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt
    19201 tcgaaacaat ggtgttcctc tgccaactcc gaaccataag tcataatatc tataaaacgg
    19261 gaagagctgt tttttgaaat tgtttcttat cactcagtgt taaacggtag gagaaaacct
    19321 cttttttatt gatatttgat tttcatcaaa cagtcacata gtcgattgct tctgcttgcg
    19381 gaatcgtttc atctacaaag tagactttat accatgtcaa gaaactgaag attgtccaga
    19441 tttttctttg ctcttcttta cgtccttcgt gatggtcatc tagcaacttc atgatttttt
    19501 cttgatcaaa aaattctttt gcaaaatctg cttcgaataa tgctttgact tgttcgtagc
    19561 cgtgttcttc tttcaaccaa gctttgattg gcacagggaa tcctaatttc acacgattcg
    19621 accattcttc cggaagatgt ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt
    19681 gattcaataa aaacttactt ggtatttgtt gagccaattt catgacttcg atatccaata
    19741 aaggtactcg gacttccaaa gaacttgcca tagaaagttt atctgccttt aacaagatat
    19801 cttttggcat ccattgatga agatcgacat attgcatctt attgacttca tctttgattc
    19861 cttctgtttt ctcgtaatga acagtcatga tttcatccac agatggtgct gtttgatatt
    19921 ttggtgttaa cacttctgat gcttctgatt cttcgaacac tcgagcatga ccgataaaat
    19981 attcttttgc aggagcaagt gattcatata aatggatccg tccatggaag tttttcattt
    20041 ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg taattttttc aatccttgag
    20101 caatcacacg gatgaatttt gaattcgtat ggaatccata cgtttggtat cccgcaaaca
    20161 gttcatccgc cccttctcca gattggacta cccgcacact ttgtgaagca agatttgcta
    20221 aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc atctaagtga tactggatca
    20281 aagggaatgc tttgaacgac atgtctccgt cgatcactgc tgctgtatta tctaagtcca
    20341 atagttcggt tagttttctg gcttcaatcg cttcattata ggttttatca tcaaacccga
    20401 ttgagaaaga atgatctggt ttcaatacag aggttacata actggaatca accccgctgg
    20461 ataagaaaga accaacttct acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg
    20521 tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg
    20581 catcccaata ttgctgaatg tctatttttc catctttata cgtataataa tggccttcag
    20641 gcaaacgata gacatctttg aagaatgttt cgccgtttaa tggagaatat tggaaagtca
    20701 tataaggttt caacgcttct ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac
    20761 ttttgatttc tgaaccaaac ataaatgttc cgttcatttc tgcataataa tatggtttga
    20821 tcccaaagtg gtctcttgca ccgaataact cattcttttc attatcccaa atagcaaaag
    20881 caaacattcc gcggatcttt tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa
    20941 ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca
    21001 aaggctggta attatagatt tcgccattaa aaataatgat ctttgtccga tcttcattgt
    21061 aaataggctg agtaccgccc tctaagtcaa taatacttaa tcttctgaat cctaaagcaa
    21121 catgtttatc gatatattct cctgaactat taggtccacg gtgaacgatt cgatccatca
    21181 ttgtattaat aattgttttt ttgttgtcct tgtcgttaac aaaacctaca ataccgcaca
    21241 tttaactttc acatcctaat ataaaattac tgtttatttc attcatatgc atcttatcat
    21301 agaataaata gcggacgaat attttctctc ttacaatttc gtcacgtgaa aacaaaagat
    21361 gaaagtttct gtttttacag aaaataaagt cccaatatct aatttaccat gttttctttt
    21421 ttgaggctac cttattctct tcgtgttaaa aaaatattag tggatgctat ttccaaaaaa
    21481 accagcaaaa aaagacacga cctaaagatc gtgtc
//
LOCUS       DANJJW010000042        21353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000042 DANJJW010000000
VERSION     DANJJW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..152
                     /locus_tag="PWS82_001423"
     CDS             <1..152
                     /locus_tag="PWS82_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906567.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="PWS82_001424"
     CDS             complement(230..763)
                     /locus_tag="PWS82_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL0906568.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWS82_001425"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWS82_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL0906569.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWS82_001426"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWS82_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL0906570.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="PWS82_001427"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="PWS82_001427"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0906571.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="PWS82_001428"
     CDS             complement(4488..5066)
                     /locus_tag="PWS82_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL0906572.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="PWS82_001429"
     CDS             5388..5858
                     /locus_tag="PWS82_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL0906573.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="PWS82_001430"
     CDS             complement(5966..7192)
                     /locus_tag="PWS82_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0906574.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="PWS82_001431"
     CDS             complement(7946..8146)
                     /locus_tag="PWS82_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0906575.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="PWS82_001432"
     CDS             complement(8280..8888)
                     /locus_tag="PWS82_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL0906576.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="PWS82_001433"
     CDS             complement(8973..9443)
                     /locus_tag="PWS82_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL0906577.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="PWS82_001434"
     CDS             complement(9491..10405)
                     /locus_tag="PWS82_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0906578.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="PWS82_001435"
     CDS             10551..11354
                     /locus_tag="PWS82_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL0906579.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="PWS82_001436"
     CDS             11532..12479
                     /locus_tag="PWS82_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL0906580.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="PWS82_001437"
     CDS             complement(12547..12840)
                     /locus_tag="PWS82_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL0906581.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="PWS82_001438"
     CDS             complement(12868..13533)
                     /locus_tag="PWS82_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL0906582.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="PWS82_001439"
     CDS             complement(13673..14305)
                     /locus_tag="PWS82_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906583.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="PWS82_001440"
     CDS             complement(14312..15379)
                     /locus_tag="PWS82_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0906584.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="PWS82_001441"
     CDS             complement(15376..16107)
                     /locus_tag="PWS82_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL0906585.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWS82_001442"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWS82_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL0906586.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWS82_001443"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWS82_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL0906587.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="PWS82_001444"
     CDS             18270..19439
                     /locus_tag="PWS82_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0906588.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21340)
                     /locus_tag="PWS82_001445"
     CDS             complement(19706..21340)
                     /locus_tag="PWS82_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906589.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6587 a         4179 c         3366 g         7221 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat att
//
LOCUS       DANJJW010000043        21047 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000043 DANJJW010000000
VERSION     DANJJW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            158..466
                     /gene="rpsJ"
                     /locus_tag="PWS82_001446"
     CDS             158..466
                     /gene="rpsJ"
                     /locus_tag="PWS82_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL0906590.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            497..1126
                     /gene="rplC"
                     /locus_tag="PWS82_001447"
     CDS             497..1126
                     /gene="rplC"
                     /locus_tag="PWS82_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL0906591.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1154..1777
                     /gene="rplD"
                     /locus_tag="PWS82_001448"
     CDS             1154..1777
                     /gene="rplD"
                     /locus_tag="PWS82_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL0906592.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1777..2067
                     /gene="rplW"
                     /locus_tag="PWS82_001449"
     CDS             1777..2067
                     /gene="rplW"
                     /locus_tag="PWS82_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL0906593.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2106..2939
                     /gene="rplB"
                     /locus_tag="PWS82_001450"
     CDS             2106..2939
                     /gene="rplB"
                     /locus_tag="PWS82_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL0906594.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            2982..3260
                     /gene="rpsS"
                     /locus_tag="PWS82_001451"
     CDS             2982..3260
                     /gene="rpsS"
                     /locus_tag="PWS82_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL0906595.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3283..3630
                     /gene="rplV"
                     /locus_tag="PWS82_001452"
     CDS             3283..3630
                     /gene="rplV"
                     /locus_tag="PWS82_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL0906596.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3644..4300
                     /gene="rpsC"
                     /locus_tag="PWS82_001453"
     CDS             3644..4300
                     /gene="rpsC"
                     /locus_tag="PWS82_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL0906597.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4303..4737
                     /gene="rplP"
                     /locus_tag="PWS82_001454"
     CDS             4303..4737
                     /gene="rplP"
                     /locus_tag="PWS82_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL0906598.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4727..4915
                     /gene="rpmC"
                     /locus_tag="PWS82_001455"
     CDS             4727..4915
                     /gene="rpmC"
                     /locus_tag="PWS82_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL0906599.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4940..5206
                     /gene="rpsQ"
                     /locus_tag="PWS82_001456"
     CDS             4940..5206
                     /gene="rpsQ"
                     /locus_tag="PWS82_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL0906600.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5264..5632
                     /gene="rplN"
                     /locus_tag="PWS82_001457"
     CDS             5264..5632
                     /gene="rplN"
                     /locus_tag="PWS82_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL0906601.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5669..5977
                     /gene="rplX"
                     /locus_tag="PWS82_001458"
     CDS             5669..5977
                     /gene="rplX"
                     /locus_tag="PWS82_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL0906602.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6005..6544
                     /gene="rplE"
                     /locus_tag="PWS82_001459"
     CDS             6005..6544
                     /gene="rplE"
                     /locus_tag="PWS82_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL0906603.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6563..6748
                     /locus_tag="PWS82_001460"
     CDS             6563..6748
                     /locus_tag="PWS82_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL0906604.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6784..7182
                     /gene="rpsH"
                     /locus_tag="PWS82_001461"
     CDS             6784..7182
                     /gene="rpsH"
                     /locus_tag="PWS82_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL0906605.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7214..7750
                     /gene="rplF"
                     /locus_tag="PWS82_001462"
     CDS             7214..7750
                     /gene="rplF"
                     /locus_tag="PWS82_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL0906606.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7911..8267
                     /gene="rplR"
                     /locus_tag="PWS82_001463"
     CDS             7911..8267
                     /gene="rplR"
                     /locus_tag="PWS82_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL0906607.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8288..8788
                     /gene="rpsE"
                     /locus_tag="PWS82_001464"
     CDS             8288..8788
                     /gene="rpsE"
                     /locus_tag="PWS82_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL0906608.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8803..8982
                     /gene="rpmD"
                     /locus_tag="PWS82_001465"
     CDS             8803..8982
                     /gene="rpmD"
                     /locus_tag="PWS82_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL0906609.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9026..9466
                     /gene="rplO"
                     /locus_tag="PWS82_001466"
     CDS             9026..9466
                     /gene="rplO"
                     /locus_tag="PWS82_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL0906610.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9466..10761
                     /gene="secY"
                     /locus_tag="PWS82_001467"
     CDS             9466..10761
                     /gene="secY"
                     /locus_tag="PWS82_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL0906611.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10823..11470
                     /locus_tag="PWS82_001468"
     CDS             10823..11470
                     /locus_tag="PWS82_001468"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL0906612.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11661..11879
                     /gene="infA"
                     /locus_tag="PWS82_001469"
     CDS             11661..11879
                     /gene="infA"
                     /locus_tag="PWS82_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL0906613.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11912..12028
                     /gene="rpmJ"
                     /locus_tag="PWS82_001470"
     CDS             11912..12028
                     /gene="rpmJ"
                     /locus_tag="PWS82_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL0906614.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12046..12411
                     /gene="rpsM"
                     /locus_tag="PWS82_001471"
     CDS             12046..12411
                     /gene="rpsM"
                     /locus_tag="PWS82_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL0906615.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12439..12828
                     /gene="rpsK"
                     /locus_tag="PWS82_001472"
     CDS             12439..12828
                     /gene="rpsK"
                     /locus_tag="PWS82_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL0906616.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12908..13846
                     /locus_tag="PWS82_001473"
     CDS             12908..13846
                     /locus_tag="PWS82_001473"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL0906617.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13874..14254
                     /gene="rplQ"
                     /locus_tag="PWS82_001474"
     CDS             13874..14254
                     /gene="rplQ"
                     /locus_tag="PWS82_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL0906618.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14526..15365
                     /locus_tag="PWS82_001475"
     CDS             14526..15365
                     /locus_tag="PWS82_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0906619.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15341..16210
                     /locus_tag="PWS82_001476"
     CDS             15341..16210
                     /locus_tag="PWS82_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0906620.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16200..16997
                     /locus_tag="PWS82_001477"
     CDS             16200..16997
                     /locus_tag="PWS82_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0906621.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17200..17946
                     /gene="truA"
                     /locus_tag="PWS82_001478"
     CDS             17200..17946
                     /gene="truA"
                     /locus_tag="PWS82_001478"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0906622.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18279..20948)
                     /locus_tag="PWS82_001479"
     CDS             complement(18279..20948)
                     /locus_tag="PWS82_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL0906623.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         6985 a         3855 c         4541 g         5666 t
ORIGIN      
        1 gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga
       61 ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct
      121 atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat
      181 ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac
      241 tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct
      301 ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg
      361 tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat
      421 gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa
      481 taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat
      541 cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt
      601 actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga
      661 tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc
      721 tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa
      781 agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa
      841 aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca
      901 cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt
      961 taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga
     1021 agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc
     1081 taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag
     1141 gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga
     1201 aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc
     1261 aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc
     1321 tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg
     1381 atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc
     1441 ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga
     1501 aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac
     1561 aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct
     1621 agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt
     1681 aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca
     1741 aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa
     1801 cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa
     1861 gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt
     1921 aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat
     1981 gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa
     2041 atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga
     2101 aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt
     2161 ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa
     2221 accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca
     2281 aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc
     2341 aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg
     2401 gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag
     2461 gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta
     2521 ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg
     2581 gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg
     2641 gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac
     2701 aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa
     2761 ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag
     2821 ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa
     2881 ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat
     2941 tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga
     3001 aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa
     3061 agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat
     3121 ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg
     3181 taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg
     3241 ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca
     3301 tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt
     3361 gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac
     3421 aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac
     3481 tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca
     3541 atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt
     3601 catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca
     3661 tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa
     3721 agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact
     3781 tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc
     3841 aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct
     3901 aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa
     3961 aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg
     4021 tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca
     4081 agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg
     4141 atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga
     4201 agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat
     4261 tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt
     4321 gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa
     4381 gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa
     4441 atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt
     4501 aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt
     4561 aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc
     4621 gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca
     4681 atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga
     4741 aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt
     4801 gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga
     4861 agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg
     4921 gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg
     4981 cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca
     5041 ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa
     5101 cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa
     5161 acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt
     5221 cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag
     5281 tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg
     5341 tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc
     5401 aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa
     5461 atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat
     5521 tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact
     5581 acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga
     5641 cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt
     5701 tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga
     5761 taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc
     5821 tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt
     5881 tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg
     5941 tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta
     6001 ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa
     6061 atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg
     6121 tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt
     6181 gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt
     6241 acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt
     6301 cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa
     6361 aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc
     6421 agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac
     6481 agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa
     6541 ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc
     6601 tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt
     6661 ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat
     6721 tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga
     6781 tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg
     6841 gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc
     6901 ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg
     6961 atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt
     7021 atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac
     7081 ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt
     7141 gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag
     7201 gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac
     7261 agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac
     7321 tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa
     7381 cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt
     7441 tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc
     7501 acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac
     7561 accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc
     7621 gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc
     7681 ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac
     7741 tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc
     7801 tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac
     7861 gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa
     7921 aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg
     7981 gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa
     8041 ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt
     8101 caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag
     8161 ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg
     8221 tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat
     8281 accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat
     8341 taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt
     8401 tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga
     8461 agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg
     8521 ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc
     8581 tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc
     8641 tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg
     8701 cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg
     8761 caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt
     8821 actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta
     8881 ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg
     8941 atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact
     9001 agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc
     9061 acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg
     9121 acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg
     9181 gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa
     9241 agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac
     9301 tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt
     9361 agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc
     9421 tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta
     9481 aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg
     9541 tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa
     9601 aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa
     9661 aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag
     9721 cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt
     9781 cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct
     9841 gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac
     9901 gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg
     9961 ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca
    10021 ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat
    10081 attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc
    10141 ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa
    10201 tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca
    10261 gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta
    10321 caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt
    10381 aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc
    10441 ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga
    10501 agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg
    10561 atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt
    10621 gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc
    10681 gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa
    10741 tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac
    10801 acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa
    10861 aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga
    10921 tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat
    10981 ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc
    11041 tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc
    11101 aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat
    11161 ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg
    11221 tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg
    11281 cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct
    11341 ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct
    11401 aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat
    11461 tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc
    11521 aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt
    11581 tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca
    11641 agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg
    11701 tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg
    11761 ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa
    11821 cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat
    11881 tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat
    11941 gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa
    12001 tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca
    12061 ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt
    12121 ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt
    12181 cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt
    12241 gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct
    12301 taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat
    12361 gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt
    12421 gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag
    12481 aatatagaag caggtattgc acatatccat tctacattca ataacactat catcatgatc
    12541 actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga
    12601 agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca
    12661 atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa
    12721 gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact
    12781 ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc
    12841 attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa
    12901 gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta
    12961 tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct
    13021 acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg
    13081 tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt
    13141 gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga
    13201 tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat
    13261 tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt
    13321 aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc
    13381 aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt
    13441 tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac
    13501 agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca
    13561 tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa
    13621 agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt
    13681 tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa
    13741 atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa
    13801 agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag
    13861 gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat
    13921 gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg
    13981 tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt
    14041 gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga
    14101 aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg
    14161 ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga
    14221 tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata
    14281 gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct
    14341 gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc
    14401 ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa
    14461 taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag
    14521 taatcatgga gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc
    14581 ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta
    14641 ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc
    14701 cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga
    14761 tccgacagat ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag
    14821 tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag
    14881 tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg
    14941 ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac
    15001 ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag
    15061 tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc
    15121 atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg
    15181 tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc
    15241 tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc
    15301 ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca
    15361 agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa
    15421 tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc
    15481 aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg
    15541 agaacgagat attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt
    15601 tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat
    15661 tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga
    15721 aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc
    15781 aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact
    15841 tgtattggat gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat
    15901 gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga
    15961 tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag
    16021 cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt
    16081 acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt
    16141 gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca
    16201 tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg
    16261 atccccgagc taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata
    16321 attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag
    16381 tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg
    16441 tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc
    16501 atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca
    16561 tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg
    16621 agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc
    16681 tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg
    16741 ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag
    16801 tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga
    16861 ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc
    16921 attggcataa tagagacacg atggttatca ttgcttttgt attattaact gtgctactga
    16981 tctttttaag aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt
    17041 tgcaagaaag gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg
    17101 tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat
    17161 tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc
    17221 attgcgtacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt
    17281 caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt
    17341 ggttcgggaa ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat
    17401 ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat
    17461 attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa
    17521 gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat
    17581 tatgcaagtt attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat
    17641 ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa
    17701 atacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt
    17761 acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg
    17821 aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat
    17881 cgtaatgcag caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat
    17941 gaataagagg ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat
    18001 tacttctcaa aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc
    18061 gtttaagaaa gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa
    18121 aaacagcttc tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat
    18181 catctttttg ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc
    18241 ccttggtgcg gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg
    18301 tgtgtgtttc gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg
    18361 cggtcaaggt accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca
    18421 cctatcgtaa atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact
    18481 tttatcatca agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta
    18541 gctttccatc ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt
    18601 acagtcccag aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga
    18661 ttcattgcgg cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt
    18721 atttctttcg tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag
    18781 ccaagatcat agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca
    18841 gtgatcgtcg cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa
    18901 gcttgtgtat ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct
    18961 gtttctgagt aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc
    19021 aaagtaggtg tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag
    19081 tttgtctgac tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt
    19141 gcagtaaatg ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga
    19201 ccagcttggc ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg
    19261 attcctgtaa acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc
    19321 cctcttgaac ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa
    19381 gaaaacaaac tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc
    19441 agattttcca agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc
    19501 aagtcaggat aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg
    19561 ccatggatca tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat
    19621 atactcagat gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa
    19681 atccctaaga aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct
    19741 tcaggtttga tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac
    19801 aacagcccac catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact
    19861 aagaaagaga acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc
    19921 cagaacaaat tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg
    19981 tacaaaaatt tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact
    20041 aatggtagca gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca
    20101 ccaatcatat ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg
    20161 atctgactca gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc
    20221 attgaagcgc cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat
    20281 tctgaccagt ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata
    20341 aaacccatat aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc
    20401 cgtttaagat ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca
    20461 tccagccaca tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg
    20521 ctaagtgcaa catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc
    20581 aatccggcac agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg
    20641 tttggaaaaa agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat
    20701 aaataattca atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga
    20761 acatttcgga agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata
    20821 cttctgctgc ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac
    20881 ggaatcagga aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt
    20941 tttttcatat ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt
    21001 gtttttctct gcttaaaaag agtctagttt tcacaaaaaa ataaaat
//
LOCUS       DANJJW010000044        20826 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000044 DANJJW010000000
VERSION     DANJJW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            22..1101
                     /locus_tag="PWS82_001480"
     CDS             22..1101
                     /locus_tag="PWS82_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0906624.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1298..1999)
                     /locus_tag="PWS82_001481"
                     /pseudo
     CDS             complement(1298..1999)
                     /locus_tag="PWS82_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2013..2937)
                     /locus_tag="PWS82_001482"
                     /pseudo
     CDS             complement(2013..2937)
                     /locus_tag="PWS82_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3054..4133
                     /locus_tag="PWS82_001483"
     CDS             3054..4133
                     /locus_tag="PWS82_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0906625.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4165..4758
                     /locus_tag="PWS82_001484"
     CDS             4165..4758
                     /locus_tag="PWS82_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906626.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4868..5095)
                     /locus_tag="PWS82_001485"
     CDS             complement(4868..5095)
                     /locus_tag="PWS82_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0906627.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5198..7786)
                     /locus_tag="PWS82_001486"
     CDS             complement(5198..7786)
                     /locus_tag="PWS82_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL0906628.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7806..8465)
                     /locus_tag="PWS82_001487"
     CDS             complement(7806..8465)
                     /locus_tag="PWS82_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0906629.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="PWS82_001488"
     CDS             complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="PWS82_001488"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL0906630.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9238..10104)
                     /locus_tag="PWS82_001489"
     CDS             complement(9238..10104)
                     /locus_tag="PWS82_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0906631.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10213..10854
                     /locus_tag="PWS82_001490"
     CDS             10213..10854
                     /locus_tag="PWS82_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL0906632.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="PWS82_001491"
     CDS             complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="PWS82_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL0906633.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12295..13194)
                     /locus_tag="PWS82_001492"
     CDS             complement(12295..13194)
                     /locus_tag="PWS82_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0906634.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13196..13993)
                     /locus_tag="PWS82_001493"
     CDS             complement(13196..13993)
                     /locus_tag="PWS82_001493"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL0906635.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13971..14675)
                     /locus_tag="PWS82_001494"
     CDS             complement(13971..14675)
                     /locus_tag="PWS82_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL0906636.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14806..15381
                     /locus_tag="PWS82_001495"
     CDS             14806..15381
                     /locus_tag="PWS82_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL0906637.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15499..16176
                     /locus_tag="PWS82_001496"
     CDS             15499..16176
                     /locus_tag="PWS82_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0906638.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="PWS82_001497"
     CDS             complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="PWS82_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL0906639.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18239..19237)
                     /locus_tag="PWS82_001498"
     CDS             complement(18239..19237)
                     /locus_tag="PWS82_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL0906640.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19463..20116)
                     /locus_tag="PWS82_001499"
     CDS             complement(19463..20116)
                     /locus_tag="PWS82_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL0906641.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            20550..>20826
                     /locus_tag="PWS82_001500"
     CDS             20550..>20826
                     /locus_tag="PWS82_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL0906642.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         6042 a         4513 c         3713 g         6558 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgtagt ggctaactta aacttgaaat ttacggacgt aaagcttgct gccaaattag
     1321 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
     1381 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
     1441 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
     1501 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
     1561 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
     1621 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
     1681 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
     1741 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
     1801 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga
     1861 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
     1921 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
     1981 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
     2041 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
     2101 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
     2161 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt
     2221 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
     2281 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
     2341 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
     2401 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
     2461 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
     2521 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
     2581 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
     2641 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
     2701 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
     2761 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
     2821 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
     2881 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
     2941 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
     3001 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
     3061 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
     3121 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
     3181 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
     3241 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
     3301 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
     3361 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
     3421 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
     3481 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
     3541 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
     3601 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
     3661 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
     3721 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
     3781 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
     3841 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
     3901 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
     3961 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
     4021 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
     4081 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
     4141 agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
     4201 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
     4261 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
     4321 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
     4381 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
     4441 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
     4501 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
     4561 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
     4621 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
     4681 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
     4741 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
     4801 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
     4861 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
     4921 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
     4981 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
     5041 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
     5101 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
     5161 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
     5221 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
     5281 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
     5341 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
     5401 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
     5461 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
     5521 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
     5581 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
     5641 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
     5701 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
     5761 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
     5821 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
     5881 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
     5941 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
     6001 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
     6061 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
     6121 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
     6181 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
     6241 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
     6301 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
     6361 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
     6421 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
     6481 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
     6541 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
     6601 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
     6661 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
     6721 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
     6781 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
     6841 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
     6901 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
     6961 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
     7021 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
     7081 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
     7141 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
     7201 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
     7261 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
     7321 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
     7381 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
     7441 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
     7501 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
     7561 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
     7621 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
     7681 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
     7741 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
     7801 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
     7861 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
     7921 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
     7981 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg
     8041 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
     8101 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
     8161 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
     8221 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
     8281 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
     8341 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
     8401 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
     8461 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
     8521 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
     8581 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
     8641 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
     8701 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
     8761 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
     8821 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
     8881 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
     8941 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
     9001 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
     9061 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
     9121 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
     9181 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
     9241 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
     9301 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
     9361 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
     9421 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
     9481 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
     9541 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
     9601 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
     9661 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
     9721 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
     9781 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
     9841 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
     9901 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
     9961 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    10021 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    10081 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    10141 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    10201 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    10261 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    10321 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    10381 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    10441 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    10501 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    10561 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    10621 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    10681 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    10741 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    10801 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    10861 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    10921 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    10981 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    11041 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    11101 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    11161 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    11221 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    11281 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    11341 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacataccc aaaaacaaca
    11401 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    11461 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    11521 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    11581 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    11641 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    11701 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    11761 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    11821 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    11881 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    11941 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    12001 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    12061 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    12121 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    12181 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    12241 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    12301 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    12361 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    12421 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    12481 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    12541 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    12601 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    12661 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    12721 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc
    12781 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    12841 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    12901 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    12961 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    13021 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    13081 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    13141 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    13201 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    13261 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    13321 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    13381 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    13441 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    13501 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    13561 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    13621 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    13681 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    13741 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    13801 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    13861 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    13921 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    13981 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    14041 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    14101 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    14161 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    14221 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    14281 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    14341 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    14401 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    14461 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    14521 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    14581 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    14641 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    14701 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    14761 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    14821 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    14881 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    14941 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    15001 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    15061 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    15121 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    15181 cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    15241 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    15301 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    15361 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    15421 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    15481 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    15541 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    15601 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    15661 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    15721 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    15781 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    15841 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    15901 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    15961 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    16021 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    16081 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    16141 tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa
    16201 gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa
    16261 acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc
    16321 aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc
    16381 gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag
    16441 tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta
    16501 attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac
    16561 tgctggacca taatattttg cattcaattt accataacta tcacttaggt attcactagt
    16621 caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt
    16681 ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac
    16741 acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga
    16801 agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt
    16861 gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt
    16921 atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc
    16981 gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac
    17041 gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc
    17101 ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg
    17161 cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa
    17221 atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct
    17281 caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt
    17341 tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa
    17401 tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac
    17461 gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc
    17521 tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc
    17581 aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat
    17641 ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc
    17701 tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag
    17761 actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt
    17821 aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat
    17881 cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt
    17941 taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt
    18001 ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc
    18061 catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac
    18121 atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt
    18181 agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact
    18241 attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt
    18301 catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt
    18361 tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa
    18421 cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca
    18481 aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt
    18541 gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca
    18601 tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag
    18661 tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca
    18721 aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat
    18781 agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa
    18841 gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct
    18901 ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc
    18961 tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc
    19021 aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg
    19081 aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa
    19141 tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa
    19201 aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta
    19261 cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta
    19321 ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg
    19381 cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac
    19441 agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac
    19501 gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag
    19561 caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc
    19621 agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact
    19681 ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg
    19741 cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa
    19801 taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt
    19861 gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg
    19921 gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt
    19981 ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg
    20041 aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa
    20101 tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt
    20161 gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa
    20221 accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg
    20281 aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat
    20341 cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg
    20401 gaaaaactat ctttgtaata gtcttttggc tctatacttt ttgatgttgg tgaacttcac
    20461 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
    20521 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
    20581 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
    20641 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
    20701 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
    20761 tactgaatga tgtgtctgag tatcgcactt atctggagct gaagaaacaa cgatttaaat
    20821 gtaagt
//
LOCUS       DANJJW010000045        20486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000045 DANJJW010000000
VERSION     DANJJW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="PWS82_001501"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="PWS82_001501"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL0906643.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="PWS82_001502"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="PWS82_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL0906644.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="PWS82_001503"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="PWS82_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL0906645.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="PWS82_001504"
     CDS             2857..3732
                     /locus_tag="PWS82_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL0906646.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWS82_001505"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWS82_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL0906647.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="PWS82_001506"
     CDS             4629..5063
                     /locus_tag="PWS82_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL0906648.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="PWS82_001507"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="PWS82_001507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL0906649.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="PWS82_001508"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="PWS82_001508"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL0906650.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="PWS82_001509"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="PWS82_001509"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL0906651.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="PWS82_001510"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="PWS82_001510"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL0906652.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="PWS82_001511"
     CDS             13334..14260
                     /locus_tag="PWS82_001511"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL0906653.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="PWS82_001512"
     CDS             14442..15335
                     /locus_tag="PWS82_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL0906654.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="PWS82_001513"
     CDS             15469..15594
                     /locus_tag="PWS82_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906655.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="PWS82_001514"
     CDS             complement(15728..16360)
                     /locus_tag="PWS82_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906656.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="PWS82_001515"
     CDS             complement(16508..17050)
                     /locus_tag="PWS82_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL0906657.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="PWS82_001516"
     CDS             17185..17574
                     /locus_tag="PWS82_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906658.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="PWS82_001517"
     CDS             17578..18342
                     /locus_tag="PWS82_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0906659.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="PWS82_001518"
     CDS             18471..19418
                     /locus_tag="PWS82_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906660.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="PWS82_001519"
     CDS             19548..20288
                     /locus_tag="PWS82_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906661.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5496 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagttt
//
LOCUS       DANJJW010000046        20435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000046 DANJJW010000000
VERSION     DANJJW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..486)
                     /locus_tag="PWS82_001520"
     CDS             complement(<1..486)
                     /locus_tag="PWS82_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0906662.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SI"
     gene            complement(673..1323)
                     /locus_tag="PWS82_001521"
     CDS             complement(673..1323)
                     /locus_tag="PWS82_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906663.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1866..2801)
                     /gene="whiA"
                     /locus_tag="PWS82_001522"
     CDS             complement(1866..2801)
                     /gene="whiA"
                     /locus_tag="PWS82_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL0906664.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2835..3833)
                     /locus_tag="PWS82_001523"
     CDS             complement(2835..3833)
                     /locus_tag="PWS82_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL0906665.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3830..4714)
                     /gene="rapZ"
                     /locus_tag="PWS82_001524"
     CDS             complement(3830..4714)
                     /gene="rapZ"
                     /locus_tag="PWS82_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL0906666.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4849..5580)
                     /locus_tag="PWS82_001525"
     CDS             complement(4849..5580)
                     /locus_tag="PWS82_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL0906667.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5584..6849)
                     /locus_tag="PWS82_001526"
     CDS             complement(5584..6849)
                     /locus_tag="PWS82_001526"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL0906668.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPQKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7112..9928)
                     /gene="uvrA"
                     /locus_tag="PWS82_001527"
     CDS             complement(7112..9928)
                     /gene="uvrA"
                     /locus_tag="PWS82_001527"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL0906669.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9938..11932)
                     /gene="uvrB"
                     /locus_tag="PWS82_001528"
     CDS             complement(9938..11932)
                     /gene="uvrB"
                     /locus_tag="PWS82_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL0906670.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12191..13693
                     /locus_tag="PWS82_001529"
     CDS             12191..13693
                     /locus_tag="PWS82_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL0906671.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13695..14432
                     /locus_tag="PWS82_001530"
     CDS             13695..14432
                     /locus_tag="PWS82_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0906672.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14477..14650)
                     /locus_tag="PWS82_001531"
     CDS             complement(14477..14650)
                     /locus_tag="PWS82_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906673.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14626..15291
                     /locus_tag="PWS82_001532"
     CDS             14626..15291
                     /locus_tag="PWS82_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906674.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15373..16539)
                     /gene="dnaJ"
                     /locus_tag="PWS82_001533"
     CDS             complement(15373..16539)
                     /gene="dnaJ"
                     /locus_tag="PWS82_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL0906675.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16690..18519)
                     /gene="dnaK"
                     /locus_tag="PWS82_001534"
     CDS             complement(16690..18519)
                     /gene="dnaK"
                     /locus_tag="PWS82_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL0906676.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18570..19133)
                     /gene="grpE"
                     /locus_tag="PWS82_001535"
     CDS             complement(18570..19133)
                     /gene="grpE"
                     /locus_tag="PWS82_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL0906677.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19227..20270)
                     /gene="hrcA"
                     /locus_tag="PWS82_001536"
     CDS             complement(19227..20270)
                     /gene="hrcA"
                     /locus_tag="PWS82_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL0906678.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         5790 a         4446 c         3502 g         6697 t
ORIGIN      
        1 tatagattca ggaaacagta taattttagt actagataaa ttgtttacac cgattagtgt
       61 taaaatatct ttattcttct tgctaacata accctttatg cacaaaagaa cagctatact
      121 taaagtaaca aataaataac gtattccaat agaaaaatca ctataaatac tctctagcat
      181 aggaccgaca attgaagaag tcactactgc aaatgctgga cctatccaag caacattaga
      241 atgttctttc tctcttttcc tttgtaaatc tttgttgagt attaatctat ttatttcttc
      301 tgcaggcaag ttaattttca taaccttatg ggggattatc caattaaaaa tcggaaaaag
      361 gtagcctccc caaaaagtat agtgatctgt gtcaatcaga taataacctg attttgattc
      421 cagaataaca tatctcaaag ccgaacgaaa atttttcatg aatatccata atctcactgt
      481 ttccacacct aattcctcca attccaatta aatggattta tagcatcacc aattatgtta
      541 actgcctgac caacctcatt gataaaattg gcaccactat cccatgcttc aacaatgcta
      601 tatatctatc aaacaaaaaa tggaaaacta aatactcgcg cacataaatt ttacattctg
      661 ttttttataa gactaataat aatcattgaa aaaatttctt atcggtggcg catattccca
      721 attgattaat gtgatcccat attctgcaag tattttacga gcttcttcta agtcatagtg
      781 agaaaaatgc ataaaacctg caatacgatc acggactgtg agctgtggct tgcttccacc
      841 atcattcata aaatatccag cagagccact acttaaagtg attgttacat cttcactttc
      901 tattattaag aattcataat gaaatgcttt caactctcca ctattattta aaagttttat
      961 actccaactt cgtctaggtt gagttgtcac attccctcta aaacttccta taaattctgc
     1021 aatcaccgta tcttcaatca aaattctttc ctcatctatt tcatattcaa gactaaacgg
     1081 aaattctcca tatgtaatct ctggcaatgg tggtgctgta gttctccaaa taaatacaat
     1141 aactgtgaat agaaaaaatc caacgatcga tataaatgtt atgagacctg ctcgaagtaa
     1201 aacccgatat aataaaaatg ctgtatttcg ctttgctata tctagaaaaa taaaaagtac
     1261 tgcaatcata atgatagtaa aagttgtaaa aaagcacatt ttttgccctc cctttataaa
     1321 catcgattct caaattctaa accaaatctt gatagttcaa gttcaggtat aacgtatacc
     1381 aaccatacat cccaattact atttatgaac ttatgattat cactgaataa atcactccat
     1441 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
     1501 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaaaaaca
     1561 gttctcaagg ttgatcgagc agttccttcc agtttttaat taatctccag ttatagataa
     1621 atacttgatc ctatctatat caggacttct atattaagca aaagtttatg tataaaacta
     1681 aaatcttcat tcagttgtct ccccgctttt tatacgattg tattaaaaaa caatagaagc
     1741 ctttcaacat tatgttactc cagtaaagtt ggaattatag tttgacggaa gaaaagcaag
     1801 tatagctttt aaaaaaaact gatacattcg catctcctgc ttcatgtatc agttttttct
     1861 ttggattaaa ctgcttcttc acgcagtttt tccgcaaatt catttatttt gcgaattcgg
     1921 tgattaattc ccgattttga tattgcacct gagggaatca tttcccctaa ttctttcaag
     1981 ctgacctctg gatgttgaag ccgaagttct gcgatttcct gaagtttttc aggaagggca
     2041 tgtagaccga cctttgcttc gatcaattcg atgttttcga tttgtttaga agccgcatca
     2101 atcgtcttgt tcagatttgc tgtctcacaa ttgactagac gattgaccga atttctcata
     2161 tcccgtacga ttcgaacatc ttcaaatcgg agcattgagt tggttgcacc aatcaatgtt
     2221 aagaaatcag caattttttc tgctcctttt aaatacgaaa tatagccatt gcgccgttca
     2281 agcgttcgag catgtagacc gtaataattt aacatatcgc aaatgtcttg gttatgctgt
     2341 tcataaatag aaaatatttc caaatgataa cgactcgttt ccggattatt gactgaacct
     2401 gaggccataa aagcaccacg taagtaagaa cgcatttttt gcgaatttcc cataatctcg
     2461 tccgaaacgt gtccattgaa catcatacca tccatgatat caagatccat caacacatct
     2521 tttgtgcctt gcttcaaccg gacgatatag acattgttct tttttaattt cattttttta
     2581 cgaacgagta gctcacttcg tacctgataa tgatctttca aaagagaata gatacgtctg
     2641 gcaattgcag cattttctgt ttggacgttc aaaataaact gctggttcac aatacctaaa
     2701 gagccgttca ttcgcagcaa agcagctaat tccgctttcg catgttctcg atgaacttcc
     2761 aaaccagtta gttctttctt cacatctgca gcaaatgaca tcctattctc ctcctttctc
     2821 caacaataaa ttgcttaata ttttgaaccg aaaactaaac ggaacaattc ttctactact
     2881 ttgtctccat cgtggaagac accaccatct ttcaattcta aaaagtcggt ggaaatcact
     2941 cgacatcctt catttctcaa tccgttgaag tcgtgtgcga cttgtactag atactcatca
     3001 tagatctctg ggtccatata atcttcaggc actttttccg tattgaccaa tactgtatcg
     3061 ataaaaggct gattcaaatg cttgtgtaaa acacgtatat gatcggcatc tgtgaaatgt
     3121 tccgtttccc ctttttgggt catgatatta cagatataga cggtttcagc agttgtcttg
     3181 agcattgctt cgccgatttc tggtatcact aaatttggaa ggatactggt gaataaactt
     3241 ccgggtccca aaacgaccat gtcggcgtcc ataattgctg aaacgacttt tcgagccgct
     3301 ttggcttcac ttttatcatt tgtgttgcag acatacacac ggtcaatggt tttacggttg
     3361 atcgcgattt tagattctcc tactgcagtc gttccgtctt taaaaacagc atgcaaggtc
     3421 aaaggagttt ctgaagaagg ataaacatgt ccatctacat gcatcatttt tgctaataat
     3481 tgaatcgcct cataagtgct tccccgcatc tcggaaactg cggcaatgat cagattaccg
     3541 atcgtatgat tggctaaaaa ctgatcttcc tttttgaaac ggtattggaa aatatcttca
     3601 tagaattgtg gcatatccga taacgccact aagacatttc tcaagtcacc aggaggagcc
     3661 atagcgatcg attctctgat tgcaccgctg cttccaccat catctgcaac agtcactact
     3721 gctgtgatat ctgctccttg atttcgtaaa cttttcagga taactggcag accagtgcca
     3781 ccgccgacca ctacgatctt tggttttctt atccggtagg ttttcatttt cacgaccggt
     3841 tcaccgtctc tttcctttta tccttgtccc gatgtgtcac attgacttta tagtttttcc
     3901 ctaaagcatt tgccaaacgt tcagtcaatg ccacagaacg gtgttgaccg cctgtacagc
     3961 cgattgcaac tgtcaagctg ctttttcctt ctttaatgta tccaggcaat acagtttcca
     4021 gaagatccaa aaatttatgg tagaatgatt gcgtttcatc aaaggacatc acataatcat
     4081 agactggctg atcttttccg gtcaatggtc ttagttccgg gatataatga gggttgggca
     4141 aaaaacgcac atccatcaca atatccgaat cgatcggcaa cccgtattta aaaccaaatg
     4201 atacaaattc gatccgaaat ccttggtctc cctttgattt gaatgactca ttgatttttt
     4261 cacgtaattg tcgtggtgtc aatgttgtcg tatcaatgat cacggaagct tgggctttca
     4321 agtcgtctag tatcgcccgt tctttacgta tcccttcagt gatcaaaccg tccatcgcca
     4381 ttggatgtgc tcgtcttgtt tctttgtagc gagccactag ttcttcgtct gaagcatcta
     4441 aaaataagat agaagtgtcg atgaagttcg tattttcgat ttcgatcagc atgttttgga
     4501 tttcttcaaa gaaggaacga gaacgcaaat ccactcctaa agccattttt gtgactttac
     4561 ctgattcttt gatcaattcc cagaattttg gaatcaatcg aggcggcata ttatcgatac
     4621 aaaaatagcc catatcttca aaactttgga tagctactgt tttccctgca ccactcatac
     4681 ctgttatgat gactaattgc aaattatctg tcatatttac gaacccctct cacattgtgc
     4741 ataataaaat tatatcatcc cgggcgtatc attaccagtt ttctttttct tttacaaaaa
     4801 gatttggacc aaatatcttt taggatattt gccccaaatc caattttttt acttttccga
     4861 tgctgtatcc aatgcctcgc ttctttcagc taatgcacgt tcgatcgttc gatccgctaa
     4921 aatagattga gcatcgatca ctgggtaatg attatcttca gcaaattcat gcatcaaaga
     4981 taattctgtg caacctagga tgactttttc acagtttagg cgctctaccg cttcctctaa
     5041 gatttcgtaa tagagctctt gattcaaata atccgattcc ttgatctcat gatagatcag
     5101 gtaattgatt ttttcttgaa gcgcagtatc aggaatcact gtttcgaaac caagattttt
     5161 gacttcgcgt tcatagattc cagctttcat acttccttct gttcctagaa tcgctactct
     5221 ccctgtcgta tgctgacgga caagttcatt tgctgcttct cttggcatat gcaagatagg
     5281 aatatccgtt gccgcttgta actcttcaaa aaagtagtgt gcggtgttac aagtcaacac
     5341 aatgaagttt ggtcgcaata aattttgttt ctcaatatca tccagcagaa acggcattgg
     5401 attttcttct gatcgatcta aaatataagc tgtacgatca ggaactgttg catgattgaa
     5461 taagacataa ttcaaatatt cttgatcttt tgtagctttc gttcggtggt tgatcaagcg
     5521 aacaaaactt tctgtcgcca tcgtgcccat tccgcctaaa atactgaaaa aattctccat
     5581 gaactagcct tctttcacat gaaaatactt tttgaatctt ggaatatagt tatggaacat
     5641 atatttcatc aacaaccagc gcttgattga tcggtctttt tcataaaata cagtagtacc
     5701 gtaacgattt tctttgatca tttgctcagc tagttttttg tcttcatttt cccgagcata
     5761 ttctaaaaag attttttggg gcacacccat ccataatctt gctttatctg attgattcgt
     5821 tccataggtc gtttcaacaa agggtttgtt gaacactcga tcttctgtga caaaacgagc
     5881 cagattcaag ccgttcaatg tcacaaagaa actgctacga ccttgtctca aattgatttc
     5941 aaataattta tattctccat ctctaggatc atacttcata tcgaaattgg caaagcctgt
     6001 atattcgatt ttttccaaaa atgccttaat ggtttgatag attttttcgt tataatctgg
     6061 catgatcaca acgtaattac caatcgatgc tggtgtagga tcttctaata gtggatgacc
     6121 aaggcacatc atccgaactt gatgatcttc atctacatac gcatttagca cacgcatatt
     6181 actatcatca cccgggataa aatcttgtac aatcatttca cttttataac cggcttcgta
     6241 tatccggcct aaaattaaat cgaactcttc tctagtctct aaaataaaag ctttttttct
     6301 tccttcaaat tggacagaaa gatactccac actgtttgca ggtttcaaag caactgggaa
     6361 ttcaaaaggc aattcctgtt ccaaatgtcc atttactaac atttcttctc tgacgatcaa
     6421 tgttttagga tacggaagat catattcttc acaaacttca taaaagctta ctttattgat
     6481 cagcctttca aataaagaat aatcaatata tggacagata aaatattctg acagctcttg
     6541 tttatgttgg gagatcaatt ctgcatatcc atcaccacaa gcaatcaaaa gatatttttt
     6601 actcttatca gaatatcttt cttttgccaa acgaagcatc gtctcgataa atacagggtc
     6661 tttatcaaat cctggaataa cttctacatt gacaatttta ctgtagcggg taggggctaa
     6721 ctgatccgaa gcatatgctt ggcagatttt gccgtatgct tcattaaaag aacgtgccat
     6781 accataaacg ttcatgtcgc ttcctaataa tatagggata aattcttcat tttcaatact
     6841 gttcatcatt atctctcact tactcataat ctcttgtttt tcttttccaa ctacacaagg
     6901 aatatcatac cataaagaaa aaagcaatca aaggaaagtc ctccgattgc tcaattcttt
     6961 tttgaacgga ttgaacttat aggaagtttt tatctatacc aatcagtgta gaaagaacta
     7021 ccatgtaatg attctagaac actgatatta ataaaacaag caaaaattca tttttttatg
     7081 tttttttaac tatctcagct atctatcctc tttataccat tatttttttc aaatagtgac
     7141 ctgtataact ttcttttacg ttaatgattt cttctggtgt tccagtcgca aggatcgttc
     7201 ctccaccttc tccaccttct ggtcctaaat caatgatata atctgctgtt ttgattacat
     7261 ctagattgtg ttcaatcact aaaactgtgt taccagcatc tactaatctt tgtaatacat
     7321 gcaacaagcg ggcgatgtca tcgctatgaa gtcctgtcgt tggttcatct agtatataga
     7381 aattctttcc attagagatt ttgtgcaatt cactggcaag tttcatccgt tgtgcctcac
     7441 caccggacaa tgtcgttgct ggttgcccca tcgtcacata acctaagcca acatcaacaa
     7501 tcgtttgcag tttgcgatga atctttggaa tgtgcttgaa gaattctaca gcatcctcta
     7561 ctgtcatttc caaaatatca gcaatgcttt ttcctttgta atgcacttct aatgtttcag
     7621 aattatatcg tttaccatga catacttcac aaggaacata gacatcaggc aaaaagtgca
     7681 tttcgatctt aataattcca tccccgcgac aagcttcaca acgaccgcct tttacgttga
     7741 aactaaaacg ccctttctta taaccccgca tttttgcctc gttcgtttga gcaaataacc
     7801 cacggatatc atcaaataca cttgtataag tcgctggatt acttctcggc gtccggccga
     7861 ttgggctttg atcgatatcg atgatctttt cgatactttc gtagccggaa attgtcttga
     7921 atttacctgg cttagcagaa ttcttattta atttttgcgc taacgatttc tttaagatac
     7981 tattgaccaa tgtactcttt ccagaaccag acacacctgt gactgctaca aattctccta
     8041 atggaaattc aacactaata tttttcagat tgttttctga agcaccagtg atcttgattg
     8101 ctttcccatt acctttacgg cgttcttttg gtacagggat tgattttttc cctgaaagat
     8161 atttccctgt caaagaattt tcatcattag ctacttcttc aggagtgcct gcagccacga
     8221 tttgtccgcc ttgttctccg gccccaggtc cgacgtcaat caaataatct gctgcccaca
     8281 tcgtatcttc atcatgttcg actacaacga gggtgttgcc aagatcacgc attttcttta
     8341 atgagccaat cagtcgatcg ttgtcgcgtt gatgcaatcc gatcgatggt tcatctaaaa
     8401 tatacaaaac tccagaaaga ttcgatccaa tttgtgtagc caatctgatt cgctgcgctt
     8461 ctcccccaga taatgttccg gaagcgcgac tcaaagttag atagtctagt cccacatttt
     8521 ccaagaatga aagtctgtca tttacttctt ttaaaatcgg tttggcaatt actttctctt
     8581 gttcagataa cgttaagttt gagaagaaat ctactgcttt ttgaatagac aactcatttg
     8641 tttgcccgat attcgttcca ttgatttgaa cagatagagc ttggggattc aatcgataac
     8701 ctttacatgt ctgacaagtt aattctgtca tataaagacg catttgctca cgcgtgaaat
     8761 cactgttcgt atcatgataa cgacgctcga tattcttcaa taccccttcg aaagtcgttt
     8821 ctacatcgcg aacgccacca aaatcatttt cataatggaa atggaaaaga tcatctgtcc
     8881 cattcaagat gatctcctga tgctcttccg gtaattcttc gaatggtgta tctaaatcga
     8941 taccgaaact ttcacaagcc tgcgctaaca tttgaggata atactgagag ctgattggat
     9001 tccatggaac gatagcacct tgactaagtg tcttcgttct atctggaatc actaaatctt
     9061 gatcgacttc taatttgaca cctaaaccat cacagtctgg acaagctcca aaaggcgcat
     9121 tgaatgagaa cagtcttggt tctagttcgc caacagtaaa tccgcaataa ggacaggcat
     9181 agtgttcgct aaacaacatt tcttcttcac cgatcacgtc aaccagagca tagccgtctg
     9241 ctaatcgcag ggccgcctct aaagaatcaa ataggcgaga acgaatccct tctttcacaa
     9301 tgatccggtc gatgacgatc gcgatatcat gttttttgtt tttctccagc tcaggcactt
     9361 cacttaaatc atagatctca ccatcgactc gaacgcggac gtatccctct ttttggattc
     9421 gttcgaatat ttttttatgt tgtccttttt tctttaccac gatcggtgcc aacacttgga
     9481 tctttgtgcg ctcaggcaaa gccattactt gatctgccat ttgatctact gattgactgg
     9541 taatctcgat atgatcgttg ggacaaatcg gatgtcctac acgtgcaaac aacaagcgta
     9601 aatagtcatt gatttccgtc acagtaccta ctgtcgaccg aggattttta cttgttgttt
     9661 tctgatcaat agaaatcgcc ggactcaatc cgtcgatgct atctacgtcc ggcttatcca
     9721 tttgtcctaa aaactgacga gcataagcag aaaggctctc tacgtatctc cgctgccctt
     9781 cagcgtacag cgtatcaaat gctagggaac tttttccaga accggacaaa cctgtaacga
     9841 ccacgaattt atctcgtgga atcgttacat ctatattttt caagttatgg gcacgtgcac
     9901 catgaatcgt aattttatcg ttagccatgt tgtcctccta ctttgaagct tttaattcta
     9961 aaatcatatc tcgcacattc gcggctgttt caaaatccaa agcttttgcc gcatctttca
    10021 tttctttttc taatttcatc aataacgtgt ctttttcttc cttcgtcatt tcttcgtacg
    10081 agacttgtac cgcttctttt gtatcatctt ccgattcttt cgtaatagaa atcaaatctc
    10141 ggatttcttt aatgatcgtt tttggaacga tcccatgttc ttcattgtac ttctgctgaa
    10201 tcgtacgacg tcttgacgtt tcatccatcg ctagacgcat agaatctgtt actttgtctg
    10261 cgtacatgat tactttacct tcttcattcc gagcagctcg tccaatcgtt tggactaagg
    10321 aacgttcact acgtaaaaag ccttctttat ccgcatccaa gatagcaaca agggaaactt
    10381 caggtacgtc taatccttca cgaagcaaat tgattccaac taacacatca aattcaccta
    10441 atcgaagatc tcgaatgatt tctgttcgtt ccagcgtttt gatatcacta tgcaaatatt
    10501 tgactttgat ccctaattct ttgaaataat ctgtgaggtc ttctgccatt tttttcgtca
    10561 atgtcgttac aaacacccgc tggtcttttt cgactcgttc atttatttcg cctactagat
    10621 catcgatttg tcccatgatt ggacgaactt cgatcactgg gtcaagcagc cctgtcggac
    10681 ggatgatttg ttggataacg gtatctgttt gttcatgttc atatggtcct ggcgtcgcag
    10741 agacataaat gatctgattg acatgttttt caaactcttc taaccgaagt ggccgattgt
    10801 ctaaagcaga tggcaaacgg aaaccgtaat ctactagcat ctgttttctc gctcggtcac
    10861 cattatacat tcctctgatt tgaggcatgg ttacgtgcga ttcgtccgcc acgatcaaaa
    10921 aatcttcagg aaagaaatcc aatagcgtat aaggcggttc accttcttta cgtccgtcca
    10981 tatgccgaga ataattttca attcccgacg tgtagcccat ttctaacatc atttcaatat
    11041 cgtaattggt tcgctgttct aaacgttgag cttccagaag tttgttttca tttcgcaaga
    11101 cagttaaacg ctgttctaat tccgctttga tattcgctac ggcatgctcc atatgttcat
    11161 cattcgtcac aaagtgagtc gccgggaaga ttgctacgtg ttccgtttct cctaaaactt
    11221 cacctgtcag tgcattgact tctcggatac gttcgacctc atctccaaaa aactccacac
    11281 gtaaggcacg ttcatctctt gaagccggaa aaatctccac gacatcaccg cgcactcgaa
    11341 aacgaccgcg ctgaaaatct atatcatttc tttcgaattg gatactgact aagtcacgaa
    11401 tcaactgatt gcgatctagt tctgctccct gtcgaatcga aactacttgt ttttggtact
    11461 caaaaggcga gcctaatcca aaaatacatg aaacggatgc aatgacgatc acatcatttc
    11521 tttctaataa tgaactagta gccgaatgtc gtaatttgtc gatctcatca tttacacttg
    11581 aatctttttc gatatacgta tcgcttgaag gcacataagc ttcaggctga taataatcat
    11641 aataagaaac aaaatattca acagcattgt tagggaaaaa ttccttgaat tccccgtaca
    11701 gctgaccagc caatgttttg ttatgggcaa taatcagtgt tggtttattc actttctcaa
    11761 taagattgga aatcgtatat gttttccctg ttccagtcgc acctaataag atttgtgctt
    11821 ttttcccgcc gactacgcca tccactaatt ggttaatcgc ttcaggctga tcaccagccg
    11881 gttggtattt cgagacaagc tcaaagtgtc gggaggtatc tctctcaatc attcttcgtc
    11941 ctccttttca ttcattcctt ttcattttac gaccaataaa aagaaaagga aagcaaaggc
    12001 attccttact aaaaaattat accacaccga acatactttc gccacttttt tatcttatta
    12061 tacatgttat gttttataac ataaaatgtt agaaactcgc atttttttca ttttttcgag
    12121 cttacagtct tgtctgattc agcacttttc gcctataata ataaaatatt ggaaatttag
    12181 gaggttgatt atgaaaagaa agcatttatt tcactcattt gttttcttaa tgacaggact
    12241 tgtgacattt gcgtttggtc atgttgcaca agctgacaac actacaacgg cagagtcaag
    12301 cacaagcgag acaatgcaaa aaatagagcc caaaaaagac gtatacgtaa ttgccagtga
    12361 ctctgctttt gctccatttg aatttcaaaa cgcaaagggc gagtacgaag gcatcgatgt
    12421 aaaattgatg gaaaacatcg ccaaactgca aggatttaac atccgaatgg actaccgtgg
    12481 tttctctgct gctttgcaag ctttggaatc aggacaagca gacggaatga ttgccggaat
    12541 gactgtaaca gatgaacgaa agaaaagcta tgatttttct gattcttact ttgatagcgg
    12601 gatccaatta gctgtcaaaa aaggagatac ttcgatcaag tcttatgaag atttgaaagg
    12661 aaaaacagtc ggagcaaaag ttggtaccga aagtgctgaa tttttaagcg accatgaatc
    12721 tgaatatggc tattctatca aatattttga tgctgctgat caattgtacg acgggttaaa
    12781 agttggcgca atcgatgcaa tgatggatga ttatccggta atcggttatg cgatcaaaca
    12841 aggacaagaa ttggaaacac cgatcaaaaa agaatctggc ggaaaatatg catttgccgt
    12901 taaaaaagga caaaatcctg agcttcttaa tatgttcaat gaaggattga aagaattaaa
    12961 aagaacaggc gaatacgata agattattaa agaatatgta gcggatggtt cagaaacttc
    13021 cagtgagtct tccactgctg atgaatcaac atttcttgga cttatccaaa acaactatca
    13081 agcattgttg aaaggacttt ggagaacaat tctcctaact ttgatctcct ttgctttggc
    13141 tatagtaatc ggtgtgattt ttggattatt cagtgtagca ccaatacgcg ctcttcgcac
    13201 aattgctagt atttatgtcg atatcatccg aggaattcca atgatggttc ttgcgttctt
    13261 tattttcttt gggttgcctg gtatcgttgg atttacgatt cctgacttcc ttgctggtgt
    13321 tatcaccttg actcttaacg caagtgctta tatcgcagag attgttcgag gcgggatcaa
    13381 cgccgttcct gtcggacaaa tggaagcttc cagaagttta ggtctttctt ataatcggac
    13441 aatgcaaaaa atcattttgc cgcaagcgat acgcatcatg atcccatcat tcgtcaatca
    13501 gttcgttatc tcattaaaag acacaacgat catttccgca atcggtgtag tcgaattgct
    13561 gcaaactggt aagatcattg ttgcacgtac aacacaaagt acgtatgttt acttgatcat
    13621 tgcgatcatg tacttgattt tgatcacagc attgacaaaa ttagcaaaag ttttagaaaa
    13681 gaaggtgaag taagatggca gaaaaaatct tagttgagca tttagtcaaa aaatacggcg
    13741 ataacacagt attaaacgac atcaatgtat ccatcaacga aggagatgtc gtctgtatca
    13801 ttggcccttc aggttcagga aaaagtacat tcttgcgttg cttgaatcgt ttagaagaac
    13861 cttctgacgg tgaaattatt attgacggcg cccacttgat ggataaaagc acagatatca
    13921 acttggtgag acaacatatc ggaatggtat tccaacactt taacctattc cctcatcttt
    13981 ctattttgga aaacatcatt ttagcaccga tggacctgaa gaaagaatct agagaagaag
    14041 cagagaaaaa agcaatcaaa ttactgcaga cagttggtct agaagataaa aaagatatgt
    14101 atcctgaaat gctttctggt ggacaaaaac agcgtgtggc tattgctcgt gctctagcga
    14161 tgaaccctga catcatgctt tttgatgagc ctacttctgc actcgatcct gaaatggtcg
    14221 gtgatgtctt gaacgtcatg aaaaagttag cagaacaagg tatgacgatg gttatcgtta
    14281 cgcacgaaat ggggttcgct aaagaagtgg ccaatcgcgt catgttcatt gacgacggaa
    14341 actttttaga agatggaacg cctcaacaaa tctttgaaaa tccgcaaaat gaacggacaa
    14401 aagatttctt gaataaagta ttaaatattt aataaaaacc aaaaatgcac ggtcgccatg
    14461 aagggaccgt gtatttttat cttcttattt tctcacctaa tcgtatcagc cgcatcgctt
    14521 gttttcctgc acaaacaagc aatcgctcta cctgttccat cgcataagaa agtaccatcg
    14581 gttcattgat gatatcaagt acatagtcga taccaccctc acttaattga gcttgatttt
    14641 tcttctccat attatgtagt tgtgggacaa ctttctgaca atcagcaatt gtgtcaagaa
    14701 cggatcaatc ctttattgga gttgctcgaa agggtcttct cttattacgg acaggctttg
    14761 ctcactgtac atagacaaca aatcattatc ctagtcaatc ggatttccag agcctcattg
    14821 ctatcattgc ttgataaaat ccagacaaag tttaataaaa tgtatcagtt acagctaaac
    14881 tttggaattg ggtcgctttg ttatacagag caagagacac cgcaatcttt ccttcatgcc
    14941 aaacaagttt gcgaatggat agctttccat caatctgtga acgagatccg cttttttgaa
    15001 gatttagacc tcgggatcgt tttaccagca atcccgtcag atcaacgaac attatatgta
    15061 aaacgtattt tgaaaagcct gacagaagaa gaagtacatt tgttcaagaa gactttggca
    15121 tgcttcagta aaaataacgg aagcatcaaa aattgctcag aagaattatt tattcataaa
    15181 aacaccttgc aatatcgatt gaacaagttc cattctctca ctggttatac ccctagaaac
    15241 tatgacgatt atcatattct aaagctggct tttttgttgg tacaaacatg acaactacaa
    15301 aaaaaccagg cgaacatatg attgttcgtc tggtttttaa ttattaatga cttagcgttt
    15361 ttactagaaa aactacttcc gctttttccc gccaaaagca tctttaaact tatcgaagaa
    15421 accgtcttct tgctgttctt caactttgat gccgccagct tcagcgaatg cacgtaatgc
    15481 ttctctttgt tgttcattta gatttttcgg tgtgatcagt tttactttga catgttggtc
    15541 gcctgttgca ccaccacgta acttcggagc gccttttcca cgtaaacgga agttcgtacc
    15601 tgtctgtgtt ccagcaggga tcttcaattt cacatctcca tggacagttg gtacttggac
    15661 ttcatccccc aaagctgctt gtacaaaact taaaggcaat tcatagtaaa tttcagaacc
    15721 ttcacgatca aagatatcac tttcttctac acggaagacc acatacaaat caccaaatgg
    15781 tccgccgttt tctccagctt ctccttgacc agctaaacgc atttgttgtc cttcttctac
    15841 accagctggt acattcactt taactgaatg tgctttcttc tcatgtcctg agccatgaca
    15901 ggttggacaa ggttctttga tttctttccc tgtcccatga caaacatcac aagtttgacg
    15961 gctcatcact cgtccaagtg gtgtttggcg ttctacatta atcgtacctg caccatgaca
    16021 tttatgacaa gtagttggtt gtgtgcctgg ttttgcaccg ttcccaccac aagtatgaca
    16081 gatttcttcc cgattatact tgatttcttt ttctacacca aaaatcgctt cttcaaaaga
    16141 caagtcgacc gtatattgca aatcagcacc ttgacgaggc gcatttggat cgactgaccg
    16201 accgcctcct ccaaagaaag agtcgaagat atcttcaaaa ccaccgaagc cgccaccaga
    16261 gaagccgccg aatccaccaa atccgccacc agctccacca gcaccgtaat ttggatctgt
    16321 gcctgcatgc ccgtattggt catatgcagc gcgtttttgc gggtcactta aaatctcata
    16381 tgcttctgat acttctttga atttttcttc cgcatcaggt tctttattga tgtcaggatg
    16441 gtattttttc gaaagcttac gataagcttt ctttatttca tcttctgatg ctccttttga
    16501 gagccctaag acttcataat aatcacgttt tgttgccata ggctttcctc ctattggttc
    16561 tcatcatttt cacgtcgctt gttatcatat catattttgt ttgaaacaaa aaccgatact
    16621 tcaaactatt tttcaaaaga aaaagccaaa gtagcaactt tggccttctc tcgaaattca
    16681 gtgaactgat tatttatcgt cgccatcgac ttcttcaaaa tcagcatcaa cgacatcatc
    16741 tgctccgcct tgagctgctt ctgggttttc ttgagcttgt tgttgtgccg cttgttcgta
    16801 tagtttaaca gtcaagtttt gaacgatttc gttcaatgca tcgcgtttag ttttcatttc
    16861 ttcgatgttg tttgcttcaa cagcggcttt caattcatcg cgagctgttt ctgctttttt
    16921 cacttcttct tcgtccactt tgccttctaa ttctttcaat gttttgtcca cagagaatag
    16981 caatgcatct acatcattac gtaggtcaac ttcctcttta cgagctttat ctgcttctgc
    17041 atttgcttct gcatctttta ccatacgttc gatttcttcg tctgttagac ctgaagaaga
    17101 tttgatcgtg attttttgtt ctttttgtgt acctagatct ttcgcagata catttacaat
    17161 cccgttttta tcaatatcga atgttacttc gatttgtgga ataccacgag gagctgctgg
    17221 aatatctgtc aattggaatc ttcctagagt tttgttgtca gcagccattg ggcgttcacc
    17281 ttgtaataca tggatgtcta ctgctggttg attatctgcc gcagttgaga atacttgtga
    17341 tttacttgtt ggaatcgttg tattgcggtc aatcaattta gtgaacactc cacccattgt
    17401 ttcgatacct aatgacaatg gtgtaacgtc aagtaaaaca acatctttca catcaccagt
    17461 gatcacgcca ccttggatag cagcacccat agcaactact tcatcagggt taactgattt
    17521 gtttggttct tttcctgttt ctttacgtac tgcttctaca actgcaggga tacgagtaga
    17581 tccaccgact aaaataactt cgtcgatttc tgattgtgat aaacctgcat cttttagcgc
    17641 ttgacgtact ggtacttttg tacgttctac taagtcagct gttaattcat caaattttgc
    17701 acgtgtcaat gtcatttcca aatgcaatgg tccagattct cctgctgtga taaatggcaa
    17761 gctgatttgt gtgcttgata cgccagataa atcttttttc gctttttcag cagcgtcttt
    17821 caagcgttgt aaagccattt tgtcttgaga caagtcaatg ctgttttctt ttttgaattc
    17881 tgctaccatg tagtcgatga ttttgttatc aaagtcgtcc ccacctagat ggttgtcgcc
    17941 agctgttgac aatacatcaa agacaccgtc tcctagttca aggatagata cgtcaaaagt
    18001 accgccacca aggtcgaata ctaagatttt ttcgtcgcga tctgttttat ctaaaccata
    18061 ggcaagagca gctgcagttg gttcattgac gatacgttct acttctagac cagcgatctt
    18121 accagcatct tttgttgctt gacgttgtgc atcattaaag taagctggca cagtgataac
    18181 tgctttttcg actttttcac ctaaatagtc ttctgcgaat cctttgatat attgaaggat
    18241 cattgctgag atttcttgtg gtgtatatga tttaccttct acatctactt tgtaacctgc
    18301 ttcaccgata tggcgtttga ttgatgaaat cgtatttggg tttgtcacag cttgacgttt
    18361 tgcaacttcc cctacttgga tttctccatt tttgaatgat acaacagaag gtgttgtacg
    18421 gtttccttct gggttagcga tgatttttgc ttcgtttcct tccaatactg ctactgcaga
    18481 gttagtcgta cctaagtcaa taccgataat tttactcata attaacgtct ccttctttaa
    18541 ttgaaagtgt aagttttata tccgcacaat tattgtgcaa caatgaccat ggttggtctt
    18601 aacacgcgat catgtaattt gtagccttct tgtagtactt ctacaatcgt atcggctggt
    18661 gtatcttcag tcgctggaac agtttgtact gcttgatgaa gattaggatc aaaggcttct
    18721 cctttagcag ggattttttc aattccttct tcttccagtg cgttacgcaa actttccaag
    18781 accatttcca cgcctttttt caaacctgcc ccttgatcat ctgacacttc tgtggctaat
    18841 gcacgttcca ggttgtcaat tgatggaagt aattttttgg ctaaatcttg tgaacggtat
    18901 ttttgtaatt gctcacgctc attttttcct cgatttgcca tatttgcgat ttcagcacga
    18961 gcccgcaagt atttatcttc catttcttct aactcagctt ttaatttcgc tgtttcgaat
    19021 tcttccgctt cagcttctga aatacccgaa tcttctgcag cttcgacatc gatttccggt
    19081 tctggctgag catctgtcat ttctttatct agttcttctt gattttcttt cacgatacag
    19141 tcttcctttc attattcagt cagctgtttg attgctctta ctaagaaaga gagaacgatc
    19201 agctgcaaat agcttcgtta ttcacattac gagtttgaca agtcaagcga acggtagtaa
    19261 tcagcaagtt tagctgctag ttctcttcga taaacatcga ctagaccgaa catccgagaa
    19321 taaggcatac tagtaggccc taacaaggct attgtacctc ttccatgacc atctatctcg
    19381 taacttgctt gaatcatact catattctgc aataaatcat tgcctaattc agcaccaatc
    19441 ttgatatgaa tattgctatc catcggagtg atcatttgtg taagtccgtc tgagtctttc
    19501 ataaaagaaa acatcgattt gaactgatca agatcttgac ggggctcata atccaagaga
    19561 ttcatttgtc cactgacata aaccttatct tcaaaaacat gaccaagaac ggtgtcaaat
    19621 aactgagaca tcccttctgt cgtttggaaa tatttatgaa gaatcatcgg aatctccgta
    19681 cgaagtttat ggtacaccgt catcagagga tctccgacta gacggtcgtt tacaatctgt
    19741 accatttttt ctagttcctg acttcccata tattcgggaa gagtgaacac ttggctttct
    19801 acattcccca tatctgttac gacgatagca ataatctgtc gattattcaa gggtacgata
    19861 cgaaagccag tcagtcggcg gtcttttatt tcaggtccca atgaaaaagt cgtatagctt
    19921 gtcagctcag ataatatctc agcagattgc ttaataatct cattgatttc atgaaattct
    19981 ctgcctagag actgacgaat cagcagcaaa tcatcttctg ctacgcgagc aggttttaac
    20041 aaatgatcca cgtaataacg atagcctaaa gcagatggaa tccgcccaga agaagagtga
    20101 gtcttcaaca gcaatccttc atcttctaat gctttcatat cattgcgaat cgttgctgag
    20161 ctggccttga ctccctcaga catcaatgtt ttggaaccaa cgggcacgcc actactcgta
    20221 tagtgttgga tgatcagacg taaaatgtcg ttttgtcgct ttgttaacat cttcctcacc
    20281 tctcattagc actcatatat tctaagtgct aatccactta taaatatagc aagaagccct
    20341 tctcctgtca agagaaagac tccatttttt agcactctaa acacaagagt gctaattcta
    20401 aataattgat gtaaatccag ttatatcaac gtttc
//
LOCUS       DANJJW010000047        19997 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000047 DANJJW010000000
VERSION     DANJJW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..626)
                     /locus_tag="PWS82_001537"
     CDS             complement(<1..626)
                     /locus_tag="PWS82_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0906679.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="PWS82_001538"
     CDS             899..3370
                     /locus_tag="PWS82_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0906680.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="PWS82_001539"
     CDS             complement(3473..5209)
                     /locus_tag="PWS82_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL0906681.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="PWS82_001540"
     CDS             complement(5206..5910)
                     /locus_tag="PWS82_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906682.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="PWS82_001541"
     CDS             6041..6544
                     /locus_tag="PWS82_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL0906683.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="PWS82_001542"
     CDS             6504..6842
                     /locus_tag="PWS82_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL0906684.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="PWS82_001543"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="PWS82_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL0906685.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="PWS82_001544"
     CDS             8393..8971
                     /locus_tag="PWS82_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL0906686.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="PWS82_001545"
     CDS             8975..9496
                     /locus_tag="PWS82_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0906687.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="PWS82_001546"
     CDS             complement(9575..10129)
                     /locus_tag="PWS82_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0906688.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="PWS82_001547"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="PWS82_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL0906689.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="PWS82_001548"
     CDS             10669..10920
                     /locus_tag="PWS82_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL0906690.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="PWS82_001549"
     CDS             11045..11836
                     /locus_tag="PWS82_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906691.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="PWS82_001550"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="PWS82_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL0906692.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="PWS82_001551"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="PWS82_001551"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL0906693.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="PWS82_001552"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="PWS82_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL0906694.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="PWS82_001553"
     CDS             complement(13893..15029)
                     /locus_tag="PWS82_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0906695.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="PWS82_001554"
     CDS             complement(15145..16059)
                     /locus_tag="PWS82_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0906696.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="PWS82_001555"
     CDS             complement(16150..16578)
                     /locus_tag="PWS82_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0906697.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="PWS82_001556"
     CDS             complement(16583..16957)
                     /locus_tag="PWS82_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0906698.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17243..17428
                     /locus_tag="PWS82_001557"
     CDS             17243..17428
                     /locus_tag="PWS82_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0906699.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17440..17688
                     /locus_tag="PWS82_001558"
     CDS             17440..17688
                     /locus_tag="PWS82_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906700.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17719..17898
                     /locus_tag="PWS82_001559"
     CDS             17719..17898
                     /locus_tag="PWS82_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906701.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17941..18411)
                     /locus_tag="PWS82_001560"
     CDS             complement(17941..18411)
                     /locus_tag="PWS82_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906702.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18498..19196
                     /locus_tag="PWS82_001561"
     CDS             18498..19196
                     /locus_tag="PWS82_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0906703.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19374..19715
                     /locus_tag="PWS82_001562"
     CDS             19374..19715
                     /locus_tag="PWS82_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906704.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19708..>19997
                     /locus_tag="PWS82_001563"
     CDS             19708..>19997
                     /locus_tag="PWS82_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0906705.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6561 a         3509 c         3933 g         5994 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
    17161 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
    17221 tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc gagcggagat
    17281 gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt caggagctta
    17341 tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac
    17401 agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact aaaaaaacaa
    17461 gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat gtcggaacta
    17521 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
    17581 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
    17641 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
    17701 tttgagaggt gaaggttaat gggtaaattc aacagagcat tggtattcag cgcaccgcta
    17761 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
    17821 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
    17881 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
    17941 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
    18001 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
    18061 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
    18121 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
    18181 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
    18241 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
    18301 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
    18361 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
    18421 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
    18481 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
    18541 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
    18601 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
    18661 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
    18721 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
    18781 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
    18841 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
    18901 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
    18961 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
    19021 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
    19081 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
    19141 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
    19201 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
    19261 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
    19321 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
    19381 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
    19441 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
    19501 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
    19561 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
    19621 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
    19681 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
    19741 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
    19801 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
    19861 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
    19921 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
    19981 ggacacacgg aaaccga
//
LOCUS       DANJJW010000048        19985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000048 DANJJW010000000
VERSION     DANJJW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            118..672
                     /locus_tag="PWS82_001564"
     CDS             118..672
                     /locus_tag="PWS82_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0906706.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(845..1318)
                     /locus_tag="PWS82_001565"
     CDS             complement(845..1318)
                     /locus_tag="PWS82_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0906707.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1330..1866
                     /locus_tag="PWS82_001566"
     CDS             1330..1866
                     /locus_tag="PWS82_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0906708.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1906..2277
                     /locus_tag="PWS82_001567"
     CDS             1906..2277
                     /locus_tag="PWS82_001567"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0906709.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2561..3832
                     /locus_tag="PWS82_001568"
     CDS             2561..3832
                     /locus_tag="PWS82_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0906710.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4077..5102
                     /locus_tag="PWS82_001569"
     CDS             4077..5102
                     /locus_tag="PWS82_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906711.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5396..6505
                     /locus_tag="PWS82_001570"
     CDS             5396..6505
                     /locus_tag="PWS82_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL0906712.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6588..7484
                     /locus_tag="PWS82_001571"
                     /pseudo
     CDS             6588..7484
                     /locus_tag="PWS82_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7500..8324
                     /locus_tag="PWS82_001572"
     CDS             7500..8324
                     /locus_tag="PWS82_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906713.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8356..9666
                     /locus_tag="PWS82_001573"
     CDS             8356..9666
                     /locus_tag="PWS82_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL0906714.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9814..11073
                     /locus_tag="PWS82_001574"
     CDS             9814..11073
                     /locus_tag="PWS82_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0906715.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11083..11955
                     /locus_tag="PWS82_001575"
     CDS             11083..11955
                     /locus_tag="PWS82_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906716.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11967..12797
                     /locus_tag="PWS82_001576"
     CDS             11967..12797
                     /locus_tag="PWS82_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906717.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12837..15020
                     /locus_tag="PWS82_001577"
     CDS             12837..15020
                     /locus_tag="PWS82_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL0906718.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15081..16301
                     /locus_tag="PWS82_001578"
     CDS             15081..16301
                     /locus_tag="PWS82_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL0906719.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16298..17758
                     /gene="gtfA"
                     /locus_tag="PWS82_001579"
     CDS             16298..17758
                     /gene="gtfA"
                     /locus_tag="PWS82_001579"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL0906720.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17835..18941
                     /locus_tag="PWS82_001580"
                     /pseudo
     CDS             17835..18941
                     /locus_tag="PWS82_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19263..19472
                     /locus_tag="PWS82_001581"
     CDS             19263..19472
                     /locus_tag="PWS82_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0906721.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19465..19614
                     /locus_tag="PWS82_001582"
     CDS             19465..19614
                     /locus_tag="PWS82_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL0906722.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19768..>19985
                     /locus_tag="PWS82_001583"
     CDS             19768..>19985
                     /locus_tag="PWS82_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0906723.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEE"
BASE COUNT         6846 a         2997 c         3889 g         6253 t
ORIGIN      
        1 ataaactaaa tcccttgatt ttattgactt tcaaaagttt atatatataa gttaatgatt
       61 atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga aacaatagtg
      121 agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca gttttggcta
      181 agaagaacaa gtaatccgga aagagatagt tttttatttt tgtttggaat caataatggg
      241 ttacgtatgt cagatattat aggattaaaa gttggagata tacgaggcaa aagtaagcct
      301 attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa tttgagagaa
      361 gaaattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa
      421 caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat agcagaaaaa
      481 ttaggacgag atgatatagg aacacataca ttaagaaaaa cttttgggta tcactactac
      541 aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc gcaagcaatt
      601 acaaaaaggt acattggtat aactgaagat gaaataggag catcacttcg tggatttaaa
      661 ctaggtgtat aatttaaaaa tttttataaa cagtaaagac aaagattaaa agctattttt
      721 atctagaacg attttatttc ataaagttcg cggaataaaa gctggggtta ctggtacttg
      781 gtataggttc gtgttatata aactaagtaa ttaaataaca tttttaggtt ggaaaaggat
      841 gaaattaaaa aaagagaatc aatacggtct caaaaacgca aacgctgcgg aataagaaaa
      901 atactgataa ctcaacgttt agcagactat ctaataggga tagtctgctt ttttcgtttt
      961 tgaccatact tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat
     1021 ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc gatatgggtg tatatatcat
     1081 ccatagtggt tttaatatct gtatgtccaa gtcgaacata cacttctttg acagacgcac
     1141 cagattcaaa gagcagacta caatgggtat gacgaaaacc atgaagtgtg atatgtttta
     1201 agttattctc tttaatgatg attttcaaat tgtgatttac atagtctaaa taaaggggct
     1261 tattttcttc tgatgaaaac aaaagttgtt caccatcttt tataggtttg attctcattt
     1321 ttagatatta tgcccgcacg tattcatcac atttctattg tgaatcgctt tattcgaccg
     1381 acatttgact tttatcataa tatactcggt ttaaaattac taatgaaaac aattaatcaa
     1441 gacgatcata ccatgtatca cctgtttttt tcagataatc accaccgtgt gggaactgaa
     1501 ctgacttttt ttgaagtaca agaagggaac aaccagtttt ttggaaccaa tacgattgaa
     1561 cgaacgatac taaaagttcc ttccgaagca tcattgcatt tttgggaaat atattttgaa
     1621 acacaaggcg tttgccatta tggaatcgaa tcattttctg gacgacctat cttacgtttt
     1681 gaagggccag atgcaacacg attagcttta gttcccttac gcgaatttga agatattgat
     1741 gattatttcc cgtatgtcac agaccagatc cctacagagc atgccatttt aggaattgat
     1801 gccattcaac tacgcgtaca atattccgat gcgactgaac gagagttatt gtcggtaggc
     1861 tggtagcaca aagaatacgt cagtttcttt tcgggtcatc aacgagtgac aatcttagaa
     1921 aatcaagaca ctcagttcta tcaagaagtt cagattattc aagatcgtga gaatccagtt
     1981 gccattgaag ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat
     2041 ttacaacgca tcgacaaaca attacaagag cgcaatttta taaattcagg aataaaagat
     2101 cgtgaatttt ttgtatcact ttattatcgt gacccaaatc aattattgat tgaaattgct
     2161 acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa
     2221 attcctttat ttttaccgca gtacttaaat ttcattcgtg aacaagtgga gcaataatta
     2281 gctaatcaaa tccatgacga agagtaagag gttgactcat aagtcaaaaa ataaagaaaa
     2341 acgcatcaaa tccattctaa agaatgttga tttgatgcgg ttcctatttt tataaactat
     2401 cattttcgac taataaaact acttatgagt cagcctctct ttttttcttg atttttatac
     2461 ctctttctag gtaaactaaa catctgccaa aaataccgtg tctcattgaa acattctcaa
     2521 ctatttcaaa ataaagggga gtaccaaatg aacgagtata ttgaaattat ccacgcaaga
     2581 gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta tttttaccag
     2641 cgtatctggt tcaggaaaat catcgattgt ttttgatact attacccaag aagctggtcg
     2701 tcaactgaat gacacctatg atagttttac ccgtttattt ttacctaaat acagtcgtcc
     2761 agaggttgat gagattgata atctttctac ggcgattgtg gtcaatcaaa agaagattgg
     2821 tggcagtgcg cgctcaacgt tagggacgat tactaacatc aatgcgttat ttcgtgtgtt
     2881 gttttcatgt tttgcaacac ctagtttagg atatgcaaat gcttattcat ttaatgatcc
     2941 tagtggaatg tgttctgtct gtcagggaat cggtaagacg attgcatttt taataggatt
     3001 gctttatttt tcggctattg ataatgctga ttttgtattt tctgatccat ttaccgcaga
     3061 agttttgtct ggaattgagg aacaggcaaa gaatcatggt tattttgtat tggtgcataa
     3121 tgttaattca gcagaagatg ttaaaactat tcagagaaat tggcgttttg aggggttcat
     3181 tgttgttggt gtacgtgtaa aagaattttt ggaattaaat aacgtttttg atggtcctgt
     3241 tgtttatatt gatacgcatc tttcaaaaga gacattaaga gaacttgaac aacaaatgaa
     3301 tcgtttattt gtaaatacaa atgattacca agctagtaca ttggcagtgg aacatttgat
     3361 tcattatggt catgagaaaa tagcgtttct ttcttttgac tacattgtag gtgaaccaag
     3421 tgttattcaa gaacgacatc aagcttatat cgatacatta aagaaatatc atttaacctt
     3481 taaatcgcat tggatatata acaactctga atttgagcga atttatcaag aattagctga
     3541 atttactgca attgttgtta ctggagatta tttagcggct aaattagtaa aatttttaaa
     3601 aaataagaat aaacgagtga tggaacaatt atcgattatt ggatttgatg atattacttt
     3661 tgcagaatta atggatcctg cgttgacaac aatacgacta aatccaggta acaagggggc
     3721 gattgctttt caccaactga ttggactggc caaacaagaa gaaataaaaa atcaagtact
     3781 actattagag ggagaattga tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc
     3841 tgtgaaataa gtatagttaa caataaaata tccgaacgat agagagaatg aactgtgatc
     3901 tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa ttgttcggat
     3961 attttttctt gtttttaatt atgattacag tgcctatttt taacgaaatt tgcttatttt
     4021 caaaatgtat gattatatgt tagtaaacac tttcaaatat gaaaagagga gctattatgg
     4081 taggaattag ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc
     4141 taaatgagga tccgacactt tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg
     4201 ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat
     4261 taataacgac agtttctgaa gtagatgagt tggaagatcc ctattttcga acgattcgca
     4321 gagggattca agtcgaagca gaacaaagca aagtcaattt gaagaaaatt attcgtttgt
     4381 cagaaacacc gttggagcaa gacgatttgc taaaatgtca aggtgtgttg attattgggc
     4441 aggtgctatc ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg
     4501 atgaccctag tattgaagct gaagtagatg cagtttatac tgatttaaca aaagcaacga
     4561 atcagcattt agatcgattg tataaaaaag gacaccgaaa cattgcgttt atcggaggag
     4621 gacgtgttcg tttagatagc caaggaaata aacacgtgtc agatgatgat tatcgaaaaa
     4681 tagcctatga gacgtggatg aaacaaaaag gattagaaaa atatagtcaa atttatttag
     4741 aaggctggac cacacttgac ggaatgcagg cagcagattt acttttgaaa aaacatgcta
     4801 aagataagtt gccgactgct atcatggtag gaaatgatcc tattgctgtg ggggtttatc
     4861 gttcacttca aaaaaatggt ctacatattc cagaagatat ttcgattgtg agttttgata
     4921 atattgaagt tgctgaattt ttaacacctg cattaaatac ggtgaatgtg aatacagaag
     4981 aaattggaaa actagcagtt cgcatggtta atgaacgaat aaaagaagtt agaaatgttt
     5041 cgatcaaagt gattgtttct aataatatta ttgttagaga aagtgaaaaa aatatcgatt
     5101 aaaaaagtaa acatccaaaa aagggtgttt acttttttgt ttactaatat ttttagtaaa
     5161 aaatctcgac aaaacattaa gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt
     5221 caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag ggatagctgt
     5281 tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta aagacgggca
     5341 agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa gaaaaatgtt
     5401 agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa cgccttggga
     5461 tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag atgtagtaac
     5521 catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg gacttgatga
     5581 tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg ctgatttcta
     5641 tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg ggctatggta
     5701 caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat ggacatggga
     5761 aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct atggttttgc
     5821 atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg ggggagaagt
     5881 atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag aagctttagc
     5941 tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag aattacaaga
     6001 tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg aaggaaactg
     6061 gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt
     6121 acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg ccagtgcgag
     6181 tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg aggacgccaa
     6241 taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg aagacgagtg
     6301 ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg agtatggagt
     6361 ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc aaattattaa
     6421 ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg aaaaagaagt
     6481 caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg cgaaaggatt
     6541 ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa
     6601 tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt tttccaacgg
     6661 cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt ttaagttttc
     6721 aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac caaaaaatgt
     6781 ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca gtggtttcga
     6841 tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga aaagttaaag
     6901 gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca tccgcagtcg
     6961 caatgatttg gcgctggatg ttcaataatg attatggact gattaataac atgttggcta
     7021 aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt tattctatta
     7081 tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta gccggcttat
     7141 aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt ccaatccagc
     7201 agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta gttgtaacaa
     7261 cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact cctaatagta
     7321 ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca tttattttaa
     7381 atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata ttaattatta
     7441 ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta
     7501 tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta ggtggtattg
     7561 caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg gttgaatcta
     7621 cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat caacaagtac
     7681 ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt gttggacgag
     7741 tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc
     7801 cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc ggttccattt
     7861 ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca attccagcat
     7921 taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa tttttcttag
     7981 gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt tggacgatct
     8041 ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga atattcacag
     8101 cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca attgataaga
     8161 tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac ttcccacaat
     8221 taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc gttttccaaa
     8281 gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat taattagtga
     8341 ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac tatttttgca
     8401 aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca tattgcattg
     8461 tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa
     8521 aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga agcgttaaga
     8581 gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc aacagtgatt
     8641 gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac catggggatt
     8701 ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc taaagaaatg
     8761 gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc gatgttaacc
     8821 atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag cgttcaagta
     8881 tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt aaatgacttt
     8941 caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac aaaaaatggg
     9001 gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc acatccagat
     9061 tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc aagtgaacac
     9121 aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat tgacgaatta
     9181 gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc
     9241 atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg tgaatatgct
     9301 tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg aaatgtctta
     9361 aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc
     9421 gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt agctgcttta
     9481 aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac actagataaa
     9541 tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt gtctttaaca
     9601 gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt acctgaatat
     9661 aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt cttagttaaa
     9721 tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta acaaaaagaa
     9781 aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa aattatttta
     9841 gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa tcaggaagat
     9901 gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt
     9961 caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat
    10021 gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt accagatgtc
    10081 attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg ttatttagag
    10141 gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca acgttttgct
    10201 attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt ttactataac
    10261 aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa
    10321 atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc
    10381 tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg tgaagaggaa
    10441 gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag caaagaaatg
    10501 caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca aaacaactgg
    10561 caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc gttgattatg
    10621 ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt tgaagtagga
    10681 acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc aggagaccta
    10741 gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt tgtagaatat
    10801 atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt
    10861 gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga acttgctttt
    10921 actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga tttttatacc
    10981 ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc attaaatgct
    11041 ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat aaaagaaatt
    11101 ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca gatgattttt
    11161 ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac tggtctgact
    11221 tataattttg attttgtggg tttgaacaat tataagattc ttttttctga tccgaagttt
    11281 atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg acaaattggt
    11341 tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac attctttaga
    11401 gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt atttaaacaa
    11461 atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt tttacaaacc
    11521 agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt actttggcaa
    11581 ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc acaagaaatt
    11641 attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa tattgaaatc
    11701 acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg tggcttaaca
    11761 gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac aacatctttg
    11821 gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc gaatgctatt
    11881 gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa attatcgaaa
    11941 aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat atttctaaat
    12001 acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca acgctattcg
    12061 gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct aatccagcga
    12121 ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt tggaattcgg
    12181 caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg gctgcttatt
    12241 cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta tttttaattt
    12301 taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg atgagtaaaa
    12361 ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc gtaccacaga
    12421 cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg gatgaggctg
    12481 ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc ccaatgttaa
    12541 aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat gatttcatgc
    12601 taccattatt aattttaaat cgtgattcta atatgtggac actaccattg tttcaatata
    12661 attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat acagtaggaa
    12721 ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct ggaatgagca
    12781 atggagcagt gaaataacca ataaattatt ctcattataa aggagttcat aaacttatga
    12841 ttatatttga caatcacaca caaacatttc atttacaaaa taaaaaaatt agctatctat
    12901 taggaatcga agaaaataat tatttaacgc atctttattt tggaaaaaag attacgcact
    12961 attctggtgg gtatcgctac ccccgaacag atcgttcctt ctcacccaac aatgcaaatg
    13021 cgcaagatcg tttattatct ttagacacat taccattaga atttccagga tatggacatg
    13081 gtgattttag agaaccggcg cataatatta aattaggaaa cggtagtcgt gtaaatgatt
    13141 ttcgttatga tagctacgaa attcaaaaag gaaagcccag cttagcggga ttgccagcct
    13201 tatatacgga agatgatgat gaagctgaaa cgctgattat tcaattgaaa gacgcgatta
    13261 gtggattgac tttacagttg ttctacacaa tttatcaaaa tcacagtgta attattcgtt
    13321 ctacgcaatt agtaaataat agtgatgaaa cagtggagat taaccgctta gctagtcaat
    13381 caattgattt tccaaatcgt ccactcgagt taattcattt aaatggtacc tgggcaagag
    13441 aacgtcaaat gactagagaa aaaattcata cagggataaa agtattagat agtaaacgcg
    13501 gatcaagcag ccatcaacaa aatccgtttg ttgctttagt agatgccaaa acagatgaat
    13561 ttcaaggaga ggcatatgga tttaatttag tttatagcgg taatcacgaa acggtgattc
    13621 aaaaagatcc atttgaccaa acacgggtca ttatgggaat taattcattt aattttggtt
    13681 ggcaactaaa aactggcgac tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg
    13741 aaggtctgaa tacgttatca cagacgtatc atgaattgta taatcaccgt ttaattcgtg
    13801 gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca acgtattttg
    13861 attttaacga agaaaaaatc atgaatattg tcgatgatgc gaagaacttg ggcattgaaa
    13921 tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg
    13981 attgggttga aacagaaggc aaacttgctt gtggtttgga aaatttagcc aagcaagtgc
    14041 atgataacgg gatgaaattt gggatttggt ttgaaccgga aatgatttct gaagatagtg
    14101 aactatttcg agcacatcca gattgggcat tgggtattcc aggtcgtgac cgctcaatta
    14161 gtcgaaatca atatgttctt gatttttcaa gacaagatgt tcgtgataat atctatcaac
    14221 aaatgacggc gatttttgat cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa
    14281 atatgacaga agtctattct gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc
    14341 gttatatgtt agggttatat gaatttttag aaaagttaac aacgaattat ccaactattt
    14401 tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg tattacatgc
    14461 cacaaacttg gacgagcgac aataccgatg ccgtggctcg tctgaaaatt caatatggaa
    14521 ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc
    14581 aaacaggaag agaaacgagt ttaactattc gtggcaatgc tgcgatggct ggtgtttttg
    14641 gttatgagct agatctaacg caattaactt ctgaagaaaa agcagaaatt aaagaacaag
    14701 ttgcctttta taaaaagcat cgccaacttt tgcaatatgg aactttttat cgcttggaaa
    14761 gtccgtttga aggcaatgat actgcatgga tgtttgtctc aaaagaccaa acagaagcca
    14821 tggtctttta tttccgagta ttagcagaag ctgcgcatcc attagtcaca ttgaaattag
    14881 caggattagc agaagaagca acttatcaaa tggaagatgt aacatttgct ggcgatgaat
    14941 tgatgaatct cggtttttat attgactcag aactacatgg tgattatgcg acacaacgct
    15001 tttatttgaa acagatatag tagcttgaat ttacaatata agtgcaacac caaaagagac
    15061 tcattccaaa cgaagaaaat atgttttatt tacacgcatt tactaaaaag cagcctgatt
    15121 tgaattggga aaatgaagaa gtacgtgaag aaatctatgc aatgattaac tattggttag
    15181 ataaaggact aggcggattt agaatcgatg cgatattaaa catcaaaaaa aaactagagt
    15241 atggcatctt tgaatctgat ggagaagatg ggttagcctt cattgggccc tggattctta
    15301 atcagtcggg cattgaagtg tggttaaaag aaatgagaga gcggacattt aaaccacata
    15361 atagtatgac ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa tatattggtg
    15421 aagatggcta ttattcgatg gtttttgatt ttagttatac ggatattgat gtacctgaaa
    15481 ctggtgaatg gtttaaagat agccaatgga catggacgga tatgcgtacc aatattttta
    15541 cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt atatttagaa aaccacgatc
    15601 aaccacgttc aattaataaa tatattccag aagaatggat caatgattat agtaaaaaaa
    15661 tgttggcgac gctttttatg ttactaagag gtaccccttt tatttatcaa ggacaagaac
    15721 taggaatgac caacatagaa atggcatcat tagaagattt tgatgatgta gctacgcatt
    15781 ctcagtataa acgggcatta gaatatggat tggcaccaga gcaagcattg aaagctgtca
    15841 ataaacgaag tcgggataat tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca
    15901 gattctcaac ttctgataat gtatggttaa aagtaaatcc taattatcca gaactcaatg
    15961 cagaagctca gttaaccaat caacagtcgg tattcaatta ttatcatcaa ttaattaaat
    16021 taagaaaaaa tagtgtatac aaagacgtgc taatctacgg acaatttcta ccagtgaaag
    16081 ataaaaatga acatattttg ctttatgaac gccaattaga agataaaatc gtattagtta
    16141 tcttaaacgt tacagcaaca gaacaagaat attatgtaga tgaacagtac caacaagtat
    16201 taatcaataa ttatgaagat gttttattag atcaaagtcg aaaattgcgt ttacgaccgt
    16261 ttgaaagtat tgtattagct aattatggag gacaattatg aaaattaaaa acgaagcaat
    16321 gctcatcacc tatgcagata gtttaggaaa aaacatgggc gaattaaaac aagtcttaga
    16381 caaacattta caaggtgtta ttggtggtgt acatctgttg cctttttttc catcgacagg
    16441 tgatcgaggc tttgcgccga gtgattatac gacagttgat ccagctcttg gaacatggga
    16501 agatgtggaa gcattaggtg aagattatta tttgatgttt gattttatga ttaatcacat
    16561 ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac catgagcagt caccatacaa
    16621 agaaatgttt atccgtattc atgaattttt cccagaaaat cgaccaacac aagaagatat
    16681 tgatttaatc tacaagcgta aagacaaagc gccgtttcaa acggtagaat ttgcggatgg
    16741 tacgacagaa gaggtttgga atacctttgg ggaagaacaa attgatttag atgtgaccaa
    16801 agaagttgtg aaagaattta ttcgagaaac catcaaagat atggcgaacc atggctgttc
    16861 tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca atgatttctt
    16921 cgtggaacca gatatttggg aacttttaga cgaagtgcgt caagaagcag cgaaatatga
    16981 cgtcgaatta ttaccagaaa tccatgagca ttattccatt caaatgaaaa ttgccaatca
    17041 tgattattat atctatgact tcgccttgcc aatggtcact ctgtattcac tttatagtgg
    17101 aaaatcagaa cgtttagcca attggttgaa aatgagtccg atgaagcagt ttacaacatt
    17161 ggatacgcat gatggaattg gtgttgtgga cgctcgtgat ttattaacag atgaagaatt
    17221 ggattacacc tctgaggaat tgtataaagt cggggcgaat gtgaagaagg tctattcaag
    17281 tgccaattac aataatttag atatttatca aattaacagc acctattata gtgcattagg
    17341 cgataatgac cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc cagggattcc
    17401 acaaatttat tacgttgggt tattagcagg aaaaaatgat attgaactgt tggaagcaac
    17461 gaaagaaggg cgtaatatta atcgtcatta ctatgattta gccgaaattg aagaagaagt
    17521 gcaacggcca gtgattcaac aattatttgc acttttaaaa tttagaaatg aatctgcggc
    17581 gtttgatttg gatggaacga ttgaagtagc aacgccatca gaatcagaaa tagtgattac
    17641 acgtaaaaat aaaaacggta gtgatagtgc aacattaacg gctaacttgg atacgaaaga
    17701 atttatcatt gagtctaaag atataaaaat tttcactagt gttgcattaa aattttaacc
    17761 tattccatag aacaaaaaat cctttatact agcacttgga tgacttatcc aagaccaata
    17821 aaaaggactc atacatggat aagtataaaa caaatacagc atttaataaa tggttttctg
    17881 ccataaaatt aggaaaactt tctgtcaact cccaaaaatg tatttttgat tacaattcgt
    17941 atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt tctctacgca gttaatgaag
    18001 aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc caactagaaa
    18061 ccgatgtgtc atcgattagt catacccaat tgtctcgagc atttaatgca cttgaaccta
    18121 aagtgttaga agaaattttt caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa
    18181 ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc
    18241 ataagtgggc gactttccgt caaacaaaat caggtgtcaa gttacactta aactattgtt
    18301 atatggacga tactacgatg tatccaactg attttacctt aacaaatgcc catgaacatg
    18361 atctcaatca actaccagta ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg
    18421 ggtattagga ctttgaaaag atggatcaga tgcattggga tggctatttc ttcgtcactc
    18481 ggattaaaaa aaacaccaaa gttcacgtga tagatactct ggaaacatcg ccagaaacag
    18541 aaattctacg agacgagctg gttcgtttag gatctaaaac ctatctaacc gccaatttcc
    18601 gactagtcac tgttcaagat aagaacggac gtgtgtttca atttatcacc aatcgaatgg
    18661 acgtctcttc aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc gaattgtttt
    18721 tcaagcacat caaacaacac atgaccatta aaaccttctt ttctcaaagt gaaaaaggcg
    18781 tgcagaatca attgatttta acgatgattt ccgctctcct cacgtttctc atcaaattgg
    18841 agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat ttactgtttc
    18901 agccctttga atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga aatttggctc
    18961 gttacttttg gttttcagtt gaataacaga taaaaaattt tctggaaaaa gtcatcctct
    19021 gcgtcagcac agtttttttt gacttatttt cagaggaaat agttttcaga atattctaaa
    19081 atgttttatg caacactagt gatatttttt agaattagat ttctaatgtg cattttttga
    19141 gtattaagga gcaagtatac aaagatttat aaagctgatt aaatttgaat aaaaatatgc
    19201 aggaacgcta ctatttttag tagtgttctt ttttatattt gaataaaaaa tgtggtggtt
    19261 aaatggtggt tataacaaaa aatgaattaa tgaaattagg ttatgggaag tatcagtctg
    19321 agagaatcat acgacaggca aaagcaatta tggtttcaaa aagctatacc tattacgaaa
    19381 atagaagatt aggactagta cctgttgaat ctgtagaaga agttttaggt atgaagatag
    19441 aggtagagga ggattttcag aatcatggtt aattttaaaa aagataaaaa tgggaaatat
    19501 cattttactg ctagtttggg attcgacaaa cgaacaggga aaagagtaca aaaaaacgta
    19561 gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt ctaattatgg
    19621 aaaagaagca gaaacttctc aaagtactat gttgtttgag gagttttttt ataaattatt
    19681 cctaccttat tatcaaggga aagtagaaga aagaacatat aaaaatcggt tatctacaat
    19741 caaaaaaact tttcttattt ctttaaaatg gagttaagag aaatacgtcc aattgacatt
    19801 caaaagtggc aaaataattt attgagcaaa tataaaaatt cttatgtaag aaacatgtat
    19861 gggatttttc agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct
    19921 gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa
    19981 gaatt
//
LOCUS       DANJJW010000049        19977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000049 DANJJW010000000
VERSION     DANJJW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            70..1317
                     /locus_tag="PWS82_001584"
     CDS             70..1317
                     /locus_tag="PWS82_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0906724.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            1342..2526
                     /locus_tag="PWS82_001585"
     CDS             1342..2526
                     /locus_tag="PWS82_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0906725.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            2523..3320
                     /locus_tag="PWS82_001586"
     CDS             2523..3320
                     /locus_tag="PWS82_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL0906726.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(3762..4457)
                     /locus_tag="PWS82_001587"
     CDS             complement(3762..4457)
                     /locus_tag="PWS82_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0906727.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            4611..4889
                     /locus_tag="PWS82_001588"
     CDS             4611..4889
                     /locus_tag="PWS82_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906728.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            4908..6116
                     /locus_tag="PWS82_001589"
     CDS             4908..6116
                     /locus_tag="PWS82_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906729.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(6264..6629)
                     /locus_tag="PWS82_001590"
     CDS             complement(6264..6629)
                     /locus_tag="PWS82_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL0906730.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(6776..7549)
                     /locus_tag="PWS82_001591"
     CDS             complement(6776..7549)
                     /locus_tag="PWS82_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0906731.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(7723..8583)
                     /locus_tag="PWS82_001592"
     CDS             complement(7723..8583)
                     /locus_tag="PWS82_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL0906732.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(8583..10001)
                     /locus_tag="PWS82_001593"
     CDS             complement(8583..10001)
                     /locus_tag="PWS82_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0906733.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(10058..10507)
                     /locus_tag="PWS82_001594"
     CDS             complement(10058..10507)
                     /locus_tag="PWS82_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0906734.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(10952..11215)
                     /locus_tag="PWS82_001595"
     CDS             complement(10952..11215)
                     /locus_tag="PWS82_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL0906735.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(11314..12609)
                     /gene="rho"
                     /locus_tag="PWS82_001596"
     CDS             complement(11314..12609)
                     /gene="rho"
                     /locus_tag="PWS82_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL0906736.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(12606..13877)
                     /locus_tag="PWS82_001597"
     CDS             complement(12606..13877)
                     /locus_tag="PWS82_001597"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0906737.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(14042..14911)
                     /locus_tag="PWS82_001598"
     CDS             complement(14042..14911)
                     /locus_tag="PWS82_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL0906738.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(15203..16813)
                     /locus_tag="PWS82_001599"
     CDS             complement(15203..16813)
                     /locus_tag="PWS82_001599"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL0906739.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(18006..19346)
                     /gene="glnA"
                     /locus_tag="PWS82_001600"
     CDS             complement(18006..19346)
                     /gene="glnA"
                     /locus_tag="PWS82_001600"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL0906740.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(19374..19766)
                     /locus_tag="PWS82_001601"
     CDS             complement(19374..19766)
                     /locus_tag="PWS82_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0906741.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         5844 a         4168 c         3564 g         6401 t
ORIGIN      
        1 catcttccta tggtataata aataaacaat aattgtttgg cactagctga atcagaggag
       61 gaatttttta tgggtcttat caaagcagca acaagtatgg ttggcggcgg tctggcagat
      121 caatggctag aagtcatcga accagacaat atgagtgata caacggtcat gacaaaaggt
      181 gtcaaagtcc gcaaagatga caaacgtggc tccaatcgta aaggaacaga agatgtttta
      241 acagacggaa cagtcgtcca tgtctatcca aacatgatga tgcttttggt cgatggtgga
      301 aaaatcatcg attataccgc tgaggaaggt tattacacca ttaaaaatga cgcagctcct
      361 tccatgttca atggaacact gaaagaagca attgctgaaa cttttgaccg attcaaattt
      421 ggcggcgtca caccgcagaa acaacaagtt ttttatatca atctccaaga aataaaggga
      481 atcaaattcg gtacaagcgc gccgttgaac tactttgaca atttctataa cgcagaatta
      541 ttcttacggg cacatggcac ttattctatt catgtcgttg acccgatttt attttataca
      601 aacgcaatcc ctaaaaacaa aacacaagtg gaaatcaatg atatcaatga gcagtattta
      661 gctgaattcc taacagcgct acaatcagca atcaatcaga tgtctgcaga tggtcaacgg
      721 atttcttatg taccatctaa gagtttggaa ttaagcaaat acatggatac tgcattggat
      781 gattcttggc gtgaattacg tgggatggag attgtttctg tggctgtagc cagcatttct
      841 tacaccgatg attctgtgaa actgatcaat atgcgtaacg aaggggcgat gctaggtgat
      901 cctagcgtac gtgaaggata cgtgcaaggt tccattgcac gtggtatgga agcagccgga
      961 aaaaatgaag caggagcaat gaccggcttt atgggtgtag gtatgggaat gaatgcaaat
     1021 ggttcttatc tttctcaagc agctcaaaat aaccaagaac aaatcaaaca acaagcagaa
     1081 aaacaaaatc aacaaactgc acaaggaaat gcggatactt ggacatgtcc agtttgcggg
     1141 acagaaaatt ccggaaaatt ctgttccaac tgcggagctg ccaaacctgt agaaaacgtt
     1201 cagcctaagc tacaaatgcg atgcagtgaa tgcaatgaga tcgtggatct ttccaacggc
     1261 atccctaaat tctgtccaaa ctgtggtaaa ccgtttaaag gaatccccgt taattaattt
     1321 atttagaaag gtgtgcagct gatggatgtt cttacacata aatgtcctaa ttgcggcggt
     1381 ccgttgactt ttgatcccaa agaccaaaaa tttcattgcg aatactgcct gaatatctat
     1441 acagaagaag aagtcagcca atatgagcaa aagcaaaaag aagcgcgtat ggctggcgaa
     1501 gaaagcaaag aagaaccttc acctaccgaa gattttactt ttacagccca agaacagctt
     1561 gccgatatga atgaagccga gcgcaaagcg gttgacgagg caggcggttt ttcagataca
     1621 gcggacgaat ctgcagataa taccgcagcg atagatcttt ttctttgtcc caactgtggt
     1681 gcagagatcg tgactgatgc gacaactgct gcgacttatt gttactactg ccataatccc
     1741 gtcgtactat cgggacgttt aagcggagaa ttcttgccaa ataaagtact tccttttgct
     1801 gtcgagaaag aagaagcaat caacagattt ctcgcttgga ccaagaaaaa atggtttgtt
     1861 cccaaagctt ttttcaataa ggatcaaatc gataagttga caggtgtgta tttcccttat
     1921 tgggctacag atgctgaagt agatggtgcc atgcaggcga atggtacagt tatccgcata
     1981 tggcggatag gtgacatcga atacacagaa acgaagcaat ttgctgtttc aagagcaggc
     2041 aagctttctt ttaaagaact agtaaaaaat gccttatcaa aaaatacgca acaaaagatg
     2101 gtcgaaggcg tgcaaccttt cccgttagat aaagcaattg attttaaaag ccagtattta
     2161 gccgggttcc aagcagaaaa aagagatatc gaatatgagg caatcaaatc acagattcaa
     2221 tctgaattaa aagactattc agagaagctg ttaaaagata ccgcaaatgg atatacaaca
     2281 ttgaccggtg tgcgaacttc tgcttcaatc acaaacgaag taaataatta cgtattactg
     2341 ccggtatggc ttgttactta tcgaagcaat gactcaagta aaaaagtcta ctactacgca
     2401 atgaatggac aaacaggaaa ggtcagcggt gtcctgccga tcagccagaa aaaattggga
     2461 ctgaccgctc ttgggatttt cgctgggcta gcgatcctct ttctgatcgg aggttacttg
     2521 atatgatgaa aaagtttttt ttattctttc ttttgctagt aagtgttggc tggccggtgg
     2581 ctactcatgc agatacagct acggtaaatg atgaggctgg gttattcaca acagatcaaa
     2641 tccaatcttt ggaacaacag gcagaaaagc tgaatgaaaa aatcaaagga cgagtcttga
     2701 tcgtaacgac tacatctaat tcagaagaac caagagactt tgctgataat tacttaagag
     2761 acgcagtcgg aaatgatcaa aatggctcag tattgctatt agatatggga cagcgtgaaa
     2821 tctacatttc gacttctggt aatatgatcg attatttgac tgatagcaga atcgattcga
     2881 tcttagatga tgtttatgat cagatgacaa atgctgccta ttacaatgct gcaaaagctt
     2941 acctgtctaa atcagcaaca tatgtggaag acggagttcc aggaggacat tatcgtgtag
     3001 acgaagaaac tggcaaaatt acacgctata aggttttgac gacagtagaa atcatcattg
     3061 ctgttgtttt ggctcttatc cttagtcttg tcttttattt tgtgactgtt tcgcgttatc
     3121 aattgaaatc aggcatctac aaatatccat tccgagaaaa atccagtatc aaattaactg
     3181 aaaaaaccga tcgtctgaca aattcttttg tcacgacaag aagaatcccc aaaagcccac
     3241 ctcctggaag cggcggtggc ggaagtacta cccattctag cggtggcggg acttttggcg
     3301 gtggcggcag aagtttctaa aaattgctaa atgaatgttc gcaaaaaaag ggctgtgaca
     3361 aaaatcgtgt cacagcccct tttgttgcaa ataaacggcg ttcaaaagct tacatctacg
     3421 gtgataagtc cagccaagtt aaaaacataa gagactgttt ttacttggct cttcttatta
     3481 cttgatgcag aacagctttt tcacaacctc ttggtaaacg gtgttctcct accaactcct
     3541 cggtcacaag tcacaatatt catcaaacat gaaaagctgt tttttgaaat tgttccttgt
     3601 gactcagagt taaacggcag gctcacaacc tcttggtaaa cggtgttcaa tcagctgacc
     3661 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta
     3721 atactcaggt caaaacgctg atttcacaac ctctttgttc atcaaaagat aatattgtct
     3781 tctaacaagt ccatattcaa aataacgata tgatggtctt taacggtgat aataccatct
     3841 tttttcagtt gttttaaaat tctgtttaca ctgctagcag ttgagatgcc acaaaatttg
     3901 gcgatttctt cattcgtgat cgaaaaatcg atcagcatcc cttcttctgt ccgcacaccg
     3961 aataaatcat ataattcgta aagttgcgta caaacagctc ccagctttcc gtttaatagc
     4021 atttgctgca tttttttcat cgaataaagc agacgatagc ggtaatactc ttttacatag
     4081 tgttgtagct ttgggtcttg gttgatattt tgccaaaagc gcacacgatt gattcgataa
     4141 aactctgcat attctgattc aactcgaata ttatacggcg aatcgatgaa ttgtgaatat
     4201 tcgtctttta aaagagagac gatttcaaga tctttgatat agcgaaggtt aaattctctc
     4261 ccatcatttg aaataacact tgcttttacc gttccctctt tcaagatata cgtatgggaa
     4321 tcttccatcc cttcgaatgt caaatattta tgatatcctt tgcgtataat cggaaaatca
     4381 tttttatcca aatattcctt taagactcta ctatccaatg atgctccccc atccaaactt
     4441 tcatctaaaa agatcataca gtactttaaa actttttaaa gtccaaaggt attttactaa
     4501 tgatattaac atttgttaag tacctttttc catcacttat gcgatttttt tccttttttt
     4561 tattcgttat actgcagaag taaaattttt taagaataga gggaaaaaac atgaattact
     4621 ggatgaacac aatcattaat cgattagaaa cagcatatca aacaagattt gatatgaaag
     4681 cttctttagt ctttttaaat gatgcgtatc aaaactcgat cgaattgatc aaagcagtgg
     4741 atgagaatcc aacaaacgaa tgcgaagaat ttttaaatct gtttatgtct acaagagatt
     4801 tatttattcg ccaacttgtt gatcgctatc catccaatta tcacgatgta gaagttcaaa
     4861 tacaaaaatt aaaagcatat tcagcttaag gggaatgtat aacatacatg gaaaagaaat
     4921 tatttaataa atattttatt ggaatcacat tgatcaattt tattgtctac ctgatttatt
     4981 atctgttgat ggtcatcatg gcagtcattg cgcaagacac cttacacgcc agcttgagcc
     5041 aagcaggttt tgcttctggg atctatatca tcggaacatt gctggcacgc ttattcatgg
     5101 ggaaacagtt agaactattt ggtcggaagt ttaccttaag aggcggcgcg atcttctatc
     5161 tcctttcaac attagcatat ctttatacgc caactatcgg ggcattgtat ttagttcgat
     5221 tcttgaatgg attcggctac ggtacagttt ctacagctac aaatgctatc gttactgcat
     5281 atatccctgc atctaaaaaa ggggaaggca tcaattatta tggattaagt actagtttag
     5341 ctgccgctgt tgggccattt ttaggaatga ttttgctgaa tatgactgat tttcgtttca
     5401 ttatctggtt ttcaattgta ctggtcttct ttgttacgat tgcctgcctt gtctttcctg
     5461 taaaaaatat tccattatct gaagaacaaa aaatatctct tcgctcatgg aatatcaata
     5521 gttttattga aaagaaagca cttttcatta caggaattgc ctttttgatg ggtctttctt
     5581 attcaagtgt tttatcattt ttatcttctt atacaaaagt aatccactta gttgctgctg
     5641 ggtcattttt cttcgtcgta tatgctttgg tcatcacatt tacacgtcca ttgactggaa
     5701 ggattttcga tgtcaaagga gaacaatacg tgatgtatcc tagctattta ttcttaacag
     5761 caggattatt tttactgagt gtaacgacga atagtgtgac attgcttatc tctggagcat
     5821 tagtcggcct aggttatggt acgtttatgt caaatggaca ggctgtttgt ttaaaaatcg
     5881 tccgcgaaca tcgaatcagt attgctttat ctacctactt tattggttta gacctaggct
     5941 taggcgttgg tccttatttg atggggatgc taaaatctct tacttcattt agaggattgt
     6001 atgtgattgc cggagtcatt ccattgatat gtacagcact ctatcttgtt aatggccgaa
     6061 aacatagtca tgaagcagag ggcgaattga ttgaatcatt gaaaaatgaa atttgatctt
     6121 gatgaataga taaaaaaaac cgttcagatc tgaaaataat ctgagcggtt ttttgagtaa
     6181 aaatagttta tgattattct taattaaaca tctatttatt tttaaagctt tcatttatcg
     6241 ataagaatag tttaactgtt gatttattcc attttccgtt catctggtac gatgccttga
     6301 cttagcaacc acttgtggaa gaaccattct gccacaccga tcaatagagc aggaatcaat
     6361 actttcatcc aaatatcgac tgtatcattc ataaaatata gatagagcca caagataact
     6421 gcagctgaac cagcatctgc gatcgtcgct gcgatgttcc ctactttacg taagatcaac
     6481 agatccccta gcacatagat catgactaca ataattaccc caacaatcgt tgaatccata
     6541 aagctaacac cgtataaacc tgtcagcacg acccacagaa tcgcccagat catgattgcc
     6601 ttcactacca acgccttcac atgtttcatt ttctgcactc ctttctcctg gataaaaaag
     6661 taagattatc taacaagata accttttcat tattatacca tcggctgaga gaagcgagca
     6721 aagataaggt cctatctaaa aatgaggaag ccacttagaa cattcacgta ataatttatc
     6781 cgtacagaca ttcgatatgg tgttccttta ctttcgcata ctgttctttt gtcagttctt
     6841 tacttgtcac cagataatcg atttcatgaa aagcaatggc ttgatgcatg aatttatgtg
     6901 caattttttg ctgtgtaact aaaacggcaa gccggtctga ttgttttcta aaagcttttt
     6961 tgaccattgc ctctccttca tgagtttctg ttactccttg gctgaaatct agcccgcgac
     7021 aactcattat cgctaaatcc gcataatatg acaatgcatc gttataggct tttgaaccgt
     7081 tgattgttgc tcgtttcgga acaattgccc ctcccaatac agatactttt gccgttgtat
     7141 attcactcaa catagcagct gtgacgatgc cattcgtaat gatctgcaaa ccatcaaatc
     7201 ttgctaggtt tctgactaag tgtaaagagg ttgtactgga atccaagaat aagaccatat
     7261 catctcgaat caaagaagaa gctgtatctg caatcttacg tttttctgtt tcttcttcta
     7321 atttggtgat cttcatcagg taaggcacgt caatttttcg attgtcaata agcatggcgc
     7381 caccgcgaac tctacggatc aatccacttt tttccagaaa aacaaggtca cgtttaatcg
     7441 tggcttcgct ataatttact aactcgatca aatcggtgac tcttgcaaat tggttgtttt
     7501 ttagataatt cacaatctcc tgctgtctat tctccacttc attcaacata aaatcacccc
     7561 atttacttta tatagtaaca ggaaaaaaga ggttgtgaca gaagtgattg gcttcgagaa
     7621 ataagaagaa atttttcgaa aaaactactt ctgaacatcg tgtgtggtta agagggtatg
     7681 acaagactat tgtcataccc tcttgaccac acatttttat ttttagtata aactagcctt
     7741 gcctactgtg ttcaaaattt ccattttatg tttgaccact tcttgtacct ttttattagc
     7801 tgctggataa attgcattcg gctcgtacat cgatggattt tcactcaata tttctctcaa
     7861 agcattgaaa aagacgcttt taagatctga gctcaaattc actttggcaa ctccgtattt
     7921 tactgcttcg ctgacttctg cgtccggatt acctgaaccg ccatgtaaaa caaatggaag
     7981 atcgatttgt tggttcaatt ctttcagtaa gtcgatattt aattttggag ttctgttttt
     8041 cggatacaag ccatgcgctg ttccaattgc tacagcaagt gtatcgattc ctgtttcttt
     8101 cacaaaaatc ttcgcttgat caggatctgt ataaatgatt gtttcagcac ctccttcagc
     8161 agaaccatta ttcccaatcg ttccaagctc agcctccact gagacattta ccgcatgagc
     8221 taacgcaacc acttttttcg taatctgtac attgtcgtcg aatgatttca aagacgcatc
     8281 gatcatcacg gaagaatagc cgtttcgaat cgcccgtaag acatcttcta tcgtaccgcc
     8341 atgatccaag tggataacga tcggcagttt acttgtataa gcataggctc ttactgccgc
     8401 aacaaactca tcacttaaat aggcgatttc atcaggatga atttctaaga ttactggtgc
     8461 ttctaatctc tctgcttctt cgatgattgc tttcatcata tcatagctgc aaatattgaa
     8521 ggctggtact gcaaattgat tctctttagc cactttcaat aactctttca ttgtatataa
     8581 cattacgctt ccacctcatt tgttagattt tcatttttcg tccacttgtc ttgttttttc
     8641 tctttcactg tttttttcaa tagtccaagc ataaaggctg tagtgactgt acctgatgca
     8701 attgccaaga catacatagg taaattgttc ataacatttg gaattagcat cacccaaatc
     8761 cctccatgag gtgccattga agtaactcct actgccatgg aaatccctgc agcgactgct
     8821 gaacctgtca tcaaactagg tatcacgcgg atcgggtcag ataccgcaaa cggtatagca
     8881 ccttctgtaa tatacgaagc gcctaaaatc cagcatgatt gacctgaagt ccgttcttct
     8941 ttggtaaatt tatgtttgaa taataaagtg gctaaagcaa tacctagggg aggtgtcatt
     9001 cctgcagcca tacaagcagc aatcggtgca tttactccag ctgacatcaa accgatcgag
     9061 aaagtcgaag tcgatttatt gatcggccca cccatatcag ctgccatcat gatcccgata
     9121 agtaatccga acaatagccc ggatgtattt cccaagctat tcagccaatt tgtcaaagca
     9181 tccaacaccc atgtgatcgg ttgttcaatc acaaatacca tcaaaagccc aataattaag
     9241 atcgataaaa aggggacgac aatcagctga taaatagcag aaaattcttt ttttacgcta
     9301 attttatttg ccagaaaatc acagatatac cctgctagca cgccaccaat cattccgcca
     9361 agaaaacctg aaccacctgt agaggccagt aatccggcaa caattcctgg ggcaaaacct
     9421 gcttttccag caatcgatgt agcaatcccc gcgcctaacg caggaaccat catggcaaac
     9481 gctgtatctc ctccgatcct tgaaaatgca gctgctaaca cattatagtc tgctgagttg
     9541 ggatcagccg ctgtaatccc aaaggcaaaa ctcagtgcga tgatgattcc gcctgcgatc
     9601 acaaatggaa gcatgtaatt cacaccattc atgaaatgat tatagattgt cggctttttc
     9661 tcatttgcag ctggttcttt ttcattagac acaacgaata cgccaatatc tctcaatgct
     9721 tcgtctatta ctacttgcgg ttctttgatt gctcggcttg tagagacacg ctttactttt
     9781 ttcccggcaa atcggtccaa ttcgatttct ttatctactg caagaatgat tgcatctgcc
     9841 tctttgattt cttgttcaga taactgattt tctgttttta caccttgtgt ttcgaatttt
     9901 gcttgatggt ttagatttct tgctgttact tctaattttt ctgccgacat ataagtatga
     9961 gcaattccag tcgggcaagc agttacacca acgattttca tgattatttt cctccttttt
    10021 cttcactatc gatactcgga caaagactct actattttca ttcttgacta atctgtctga
    10081 tgatatctgc tggcgtagtg gcttctttta ttccctttct aaaatctgca ttcatcaatc
    10141 gtctggagag tgtgctgaga atctctagat gcatatctgc tgatttttca ggaactacga
    10201 tggcaaaaat atattcaatt ggcatttcat ctatcccgct atagtccgtt aatttggctg
    10261 tatgaaaaaa acacagtgct ggttttgtca catatttgct ttttgcatgg ggaatcccaa
    10321 atccatcttc tattcctgtg gagatctcct cctctcgttt gaatagtgcc ttaacgactt
    10381 tttgaacatt tcttacctta tttgtttttt tcaacacatc ccctattcgc tcaaataatg
    10441 atccttttga atctatcgat ggatcgaaaa caatcagttc ttctgcgatc aattgatcaa
    10501 acctcataaa aaacctctcc cccacaaaaa atgttaacgt tttcttggaa ctgaggtcag
    10561 tatagcatcg ctagctgtac cgttcaatat tatgaatgaa cagaggaaaa agacccatca
    10621 tccgcgtgta taagtcatta aaatgaacga tctagctcac aaacaatcgc aagtctataa
    10681 caaaacaaaa agccttcctt gaaaggaaag cttagcttct aattttaggt aaatggtttt
    10741 ttacttcgaa ataacccctc ttcatttcgt taatataagg tgtttttatc aagtggataa
    10801 ttaaaactat atactagttt ttcattgttt tgtgcattat gatcctacga ctttttcagc
    10861 atcaaacgaa gttttaaatt ggcaaaaaac ataaaaaaac agactatccc tatcagatag
    10921 tctgctaaac gttgagttat cagcgttttt attattccgc agcgtttgcg tctttgagac
    10981 cgtatttttt gttgaaacgg tccacacgtc cgtctgcttg tgtgaatttt tgacgtccag
    11041 tgtagaatgg atgtgagtca gaagttactt cgacacggat gactgggtat gtattaccgt
    11101 cttcccattc aactgtttct tgtgaagttt ttgttgaacc tgacaagaat ttgaaaccag
    11161 ttgttgaatc catgaatact actgggtggt aatctggatg gatgttttct ttcatgattt
    11221 tttcgctcct ttgccctgct ccatttgctg aaccagagtt gatttgtcga tttacaacat
    11281 taacatctta acatgtttca gtcaaggttg caattatctt ttcagatttc tggtcggatt
    11341 ttttttacca aaggagacat cttgaaattc tttgaaaaat tgctgattgt tttttgtttt
    11401 acgcaaaaag cggatcagtt gttccgtata ttctaatgaa tctccagtca tattttttcg
    11461 aagtttccaa atttcttcta attcttcact tggcatcaat aattcttctt ttcgggtacc
    11521 tgattttttg atatcgatgg ctgggaagat ccgacgttca gataattcac gtgaaagttg
    11581 taattctaga tttccagttc ctttgaattc ttcatagatc acgtcatcca tacggcttcc
    11641 tgtatcgact aaagcggtcg ctaaaatcgt caaactgcca ccttcttcga tgtttcttgc
    11701 tgccccgaaa aatttcttag gtttatacaa tgcggctggg tcaatcccgc cactcaatgt
    11761 acgtccgctt ggtggaacga ctaagttata ggcccgagca agtctagtaa tactatccat
    11821 caagataacg acatctcgtt tatcttcgac taaacgcatt gcgcgttcca atactaattc
    11881 agaaactcga gtatggtttt gcggctgcag atcaaatgta gaagatacca cgtcgccttt
    11941 cacgcttcgt tctagatccg ttacttcttc cggtcgttca tcaatcagta gaacgattaa
    12001 ttctacttcc ggatagtttt cggtgatacc atttgcgatt tcttttaata cagaagtttt
    12061 ccctgctttt ggcggtgcga cgatcaatcc acgctgacca aaaccgattg gagaaaagat
    12121 atcgatcatt cgggtagata gtctttgcgg tgttgtttcc agttgaagct gtctttgcgg
    12181 ataaagaggt gttaaggctg gaaaatgcgg acgttctttt gcttcttccg gatcttttcc
    12241 attaacgctt tccacgtgca tcaaaccata ataacgttcc gattctttag gtggacgggc
    12301 ttttcctgcc actttgtccc catttctcaa actgaatcgt ctgatttgtg aggcagaaat
    12361 atagatatct tctgcacttg gtccataatt gattggccgt aaaaaaccgt aaccatcttg
    12421 accaacgata tccaatattc cttccatata ataaaatcct tgtttctctg cttgtgcacg
    12481 aatcacagct agagataatt cctttttatt catcttactg taataaggaa ttttaaattc
    12541 tttggcataa ctataaactt cttttaacgt tttgttctct agttctgcca ttgtcaaata
    12601 atcgctcatt cagctcccac ttcctctatc atttcaattg atgcccctaa ggctgttagt
    12661 ttctcgataa tatgatcata accgcgtaag atataatcta cattgtaaat cgtcgttgtc
    12721 ccttctgcca atagtccagc aatcactaaa caagcacctg cccgaagatc agaagccacg
    12781 acttctgtgc cgtgaagttt atttggacca ttcacgatga tcatatttcc ttccacggta
    12841 atatcagcgc ccattcgtgc tagttccggc acatggttca cacgtttcgc atagatcgta
    12901 tcgatgattt ctgaagtgcc tgtagtcatc aatagtaacg cagtaagtgg ctgctgcaga
    12961 tctgtggcaa atcctggata aggataggtc atgatgtttg cgggttttaa ttctttcgag
    13021 gggtaaactt cgatttcatc ttcgctgatt ttcatatgga caccaatttc ctgcaatttt
    13081 gctataaagc tttccaaatg ttcaaagatc acatttttca ctttgatacc atttcctact
    13141 gcagctgcta atgctagata ggttcctgct tcgatgcggt caggaatcat gaaatgccga
    13201 cagccatgta atgtttcgac tccttcgata cggatcacat ctgtacccgc tcctcgaact
    13261 tttgccccca tattattcag taaagttgcc acatctatga tttccggttc tcgtgcagcg
    13321 ttttcaatta ctgtctgtcc ttttgctttt acagcagcta acatcacatt gatcgttgct
    13381 ccgactgaaa ccacgtccat gaaaatccga ttgccacgca atcctttatt ttctgtccgt
    13441 aaatacatgg caccatgttc atttgtcact tcagcaccta gagcttcaaa tcctttgacg
    13501 tgtaggtcaa tcggtctagg gcctaaataa cagccacctg gcaggcctac taccgcttcg
    13561 ccaaatttac caagcagtgt tcccataaaa taataagaag cacgcaaact attgattttt
    13621 ccgtgcggca tcggaatcga tacgacttct cgaggatcga tttctagtat gttgttggaa
    13681 aaatggacag ttgcgcccat gatctctaaa atttcaatta atgaatgaac atcttgtatg
    13741 tccggcacac cttccaagat cactggggaa tcagctaaga ttgcagctgg aatcaatgcg
    13801 actgcactat tttttgcacc gctgatcgtt acttcaccag ctaaaggtcg gtttcctttg
    13861 attacgattt ttttcatctg tttccacctt gtttattaat cttttttcat ggatatgctt
    13921 gtattttatc atagataaaa aaaagaaacg agcatggtct gccgttttaa gtaaaaaaga
    13981 atggagagcg acttcaaaag tcgttctcca ttcctatata tcgtatctgt tatgtggatg
    14041 cttacgcttt gttagctgaa ccgaaccagt cgatatgaga tttagcatct tcaacgattg
    14101 ctgctttacc tggagctaat aatttacgag ggtcgaatcc tttaccttct ttatctttac
    14161 cagcttcgat atatttacga gtagctgcag caaatgataa ttggaattca gtgttaacat
    14221 ttacttttga gatacccatt gagatagctt tttcgatttg atcttgagga atacctgatc
    14281 cgccatgtaa tactaaaggc atgtctggac ctacagcatc agcaattgct tgtaagtggt
    14341 caaaagctaa tcctttccag ttttctggat attgaccatg gatgttaccg ataccgcaag
    14401 ctaagtagtc gatacctgta gcaaccattt gtttgcattc ttcaatatca gctaattcgc
    14461 cgttaccgat gattccgtct tcttctccgc cgattgaacc aacttcacat tcaactgaga
    14521 taccttttgc atgtgctttt tcaacaacgt ctttagctaa tttcaagttt tcttcaaatg
    14581 gtagatgtga accatcgaac attactgaag tatagcctaa ttcgatacat tccaaagcag
    14641 cttcgtaatc accatggtct aagtggatag caactggaac agtgatgttc attgcttcaa
    14701 ctaaatcagt gattaaatct ttacagattt tgtaaccacc catgtattta gctgcaccca
    14761 ttgatgtttg gataagtact ggtgcttgtt ttgcttcagc cgcttcaagg attgcttgag
    14821 tccattccaa gttgtttgtg ttgaatccgc cgacagcgta accgccttta cgagcagctt
    14881 ttaaaaattc agctcctgat actactggca taataaaatt cctcctaagt tttgtaaaac
    14941 taaatttgtt aagacaattc ttaaccaacg accctatttt accaaacatt tcggtagttt
    15001 tctactaaaa tcaatgaaaa tgaaaatttt caagaagttt tttgcaaaaa ataaaatatc
    15061 caatagcgct ttaatcgaaa aaagtctttt gctttctatc ttttttccta caaataataa
    15121 caaatcagaa aaagagaaaa gtgacaagaa aattgcttat ccccttctct tgctactttt
    15181 ctctttctat caagttcaaa aattattttg tttcgtgatc tgctaatgat gctccgacaa
    15241 atgctttgat taatttttgc gggcgatttg gacgagaaat caattctggg tggaattggc
    15301 atcccacaaa gaattttttc tctggcagct ctacgatttc tactaaacgg ttatctggtg
    15361 agacacctga aaagactagc ccttgttctt caaacaattg acgatatttg ttattgaact
    15421 catagcggtg acggtgacgt tcttgcacaa catcggctcc atcatatgct tcagccgtct
    15481 tcgttccttt tttgatttta catgggtata atccaaggcg taaagttcca cccaaattct
    15541 cgatgttttc ctgatcagcc attaaatcaa taatattgtt tgctgtatct ggattcgttt
    15601 cagctgatga cgcatcttct aatccaacga cgttacgtgc aaattcgaca caagccatct
    15661 gcataccaag acaaatacct aaaaacggaa cgtcattttc gcgagcataa cgaatcgctt
    15721 cgattttccc ttctactccg cgatcaccaa atccaccagg aaccaagatt ccatctgcgt
    15781 ctttcaaacg ttcttctaca ttttctctcg ttaattcttg tgctttgatc caatcgatct
    15841 cgatgtctga attataggcg aaaccagaat gtttcaatgc ttcaactact gagatatatg
    15901 catctggtaa ctcgacatat ttaccgacaa gcgcaatccg tacttttttc ttcaagttca
    15961 agacacgttc ttctaattct ttccattctg tcatgtctgc ttctggtgta tctagtttta
    16021 aatggtcgca aacaatttga tccattcctt gtgcttgtaa aactaatggg atggagtaaa
    16081 gtgtatctac atctggcgac tcgataactg cttctggttc gacatcacaa aattgtgcca
    16141 atttgttttt cacgctttga gaaacaggtt gctctgtacg taccaccaaa atattcggtt
    16201 ggatacctaa accacgtagc tcttttacac tgtgctgcgt tggtttagtt ttcatttctc
    16261 cagcagcttt caagtaagga atcaatgttg tatggatata cataacatta tctgtaccga
    16321 catctgcttt catttgacga agtgcttcaa ggaatggcaa agattctata tcccctactg
    16381 ttccccctac ttctgtaatg atcacatctg aatcagtcat tttagctgct cgcatgattt
    16441 tttctttgat ctcatttgta atatgaggaa taacttgaac agttgctccc aagtattcgc
    16501 ccttacgttc tttacgcaaa acttcagaat agatttttcc tgtcgtcacg ttggagtatt
    16561 tgttcaaatt gatgtcaata aaacgttcgt agtggcccag atccaaatct gtttcagcac
    16621 catcatctgt tacgaatact tctccgtgct gatatgggct catcgtcccc ggatccacgt
    16681 tgatgtatgg atcaaatttt tggatagtta cttttaagcc gcggtttttc aataagcgtc
    16741 ctaaagaagc tgcaacaatc ccttttccga ttgatgaaac gacaccgccg gtcacaaaaa
    16801 tatattttgt cataagttca aaaactcctt tttaatttat gtgcaggaaa agtctcagac
    16861 aataaacaaa gctccctatt cttcaataag aatagggagc cgaaatttct gtaaacttct
    16921 gaccctctaa aagggtgccc aaggtgtata atacaagctg actaagggaa agtcaagtac
    16981 tcaaatgaat actatccgct tacaattttt tctttttcga tatcctttga gaaattattt
    17041 cttaagtcct ttatgagtgc aaaaaaaatt taacccacat aataagcttg atataaacat
    17101 agctaacgtt aaacctaagc aaatcagaaa atcgttactg aaaaatttta agtgaaataa
    17161 tagataaaaa aataaaacaa acattatgat agataatatc ccacctaaca aagaactact
    17221 ttttcctact tgcagcaaca gtttatatag cttatcgtag aaacaatctt tttctttcac
    17281 ccttatttac ctcctttacg taaaaaacgt agtccacagt tctcggattt ttggcatatc
    17341 ttttctatta aataatatca aaaacaaaac aattccacta attttttcaa agatgacctt
    17401 tttttcaaca aaagtgcctt gtagggattc attgaaggta acgaatcaca taagtaaaaa
    17461 ataaggagat gcgacaaaag tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc
    17521 aactcctcgg tcacaagtca caatatccat caaacatgag aagctgtttt tgaaattatt
    17581 tcttgtgatt cggagttaaa tggtaggctc acaacctctg gataaacggt attcaaaagc
    17641 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
    17701 tttcttaata ctcgaaacta aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa
    17761 gcttgcatct gcggtaataa gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg
    17821 gctattctta ttacttgttg cagggcagct ttttcacaac ctctccacta aacggtgttc
    17881 aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttccg
    17941 ttagtttctc taatactcag gtcaaaacgc tgatttcaca gcctctaatt cacggtattc
    18001 ctgttttaat aaagttctag atattgttct ctttcccact cggaaacagt ttgtctgaat
    18061 gccgcccatt cgagacgttt tgcttcaaca aaatttgaga agatatgttg tcctaatgcg
    18121 tcaatcatta cattatcctt acgcaattct ttgatagcat tatggatagt tgaaggtaag
    18181 tcctggatat gtgcagcttc tctttcttcc tcatccatta cataaatgtt acggtcaaca
    18241 gctgctggcg gtgtgatctg attacggata ccgtctagtc ctgcttgcaa caatactgcc
    18301 attgccaagt atgggtttgc agatggatca actgaccgca attccaaacg tgtggataaa
    18361 ccgcgagatt ctggtacacg tactaatggc gaacgattac ggccactcca tgcgacataa
    18421 acaggcgctt cataacctgg aacgagacgt ttataagaat tgactgttgg attgcaaacg
    18481 gctgtgtagg cacgagcatg tttcagcaaa ccacctaaga aatgataagc tgtttcactt
    18541 agttccaatt caccgtcttt atcatagaaa acatttcctt catcattgaa caaagacata
    18601 ttgcaatgca taccagaacc attgatccca aataacggtt tcggcatgaa agtcgcgtga
    18661 agtccatgct tgcgtgcaat cgttttgaca acgagcttga aggtttggat attatcacaa
    18721 gcatctacca catctgcata tttaaagtca atttcatgct gtcctggcgc tacttcatga
    18781 tgtgaagctt ccacttcaaa gcctagactt tctaattcca acacgatgtc acggcggcaa
    18841 ttttcaccta aatctgttgg tgcaaaatca aaataaccac ctttatcatt caaggtcgtt
    18901 gtgatcccac cgtcttcgtc taatttaaat aagaagaatt ctggttcagg tcctaaattg
    18961 aaagaggtaa aaccaaggtc gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga
    19021 tcgccggcaa atggtgtccc atccggatta tagatatcac aaattaaacg agcaactttt
    19081 ccatgagcac tttcccacgg gaagatcatc caagtggaga gatctggata caaatacata
    19141 tcgctttctt caattcgaac aaatccttca atcgaagaac cgtcaaacat cattttattt
    19201 tctaatactt tgtcgatctg gctcactgga acttctacat ttttgatcgt acctaagatg
    19261 tctgtgaaca tcaatcgtaa aaatcgtacg ttttcttcct caatgattcg tttgatttct
    19321 tcacctgtta tctgtttctt taccatggta tccctcttcc ttttttaatt tattcagtca
    19381 aagaattgtc tttgcaatcc ttgcgactga taatgacctg ttttgcttaa cccaccttgc
    19441 gccaaaatct catcacgaaa aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt
    19501 tttgcttctt gttttttcgc ctgtgccatt tcgtatacac gtttgatccc agccatattc
    19561 aatccctctg aaagatagtc tttaatctca agcaacatat caatatcatt cagcgaatac
    19621 atacgtcggt ttccttcgct tcgttccgga tgaactaaat cttgttcttc ataatagcga
    19681 atttgccgag cagacaaatc agtcagcttc ataactgtac caattggaaa tacggacata
    19741 gatcggcgca attccttttc tcccatcaga cgtccccttt cgttttataa gttggctaaa
    19801 gtgtaccaat taaaaaaaat attgtcaact gcatatgtta ggttttctaa catgagatct
    19861 ctatttttta taaaataaca aaaaaataag aacacaaaaa aggtatctag ttgattttcg
    19921 taccagatac cttttatttt ttattatact gtactatcct ttattagtgt ttttatt
//
LOCUS       DANJJW010000050        19639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000050 DANJJW010000000
VERSION     DANJJW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            13..660
                     /locus_tag="PWS82_001602"
     CDS             13..660
                     /locus_tag="PWS82_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0906742.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            744..1688
                     /locus_tag="PWS82_001603"
     CDS             744..1688
                     /locus_tag="PWS82_001603"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL0906743.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1823..3274
                     /locus_tag="PWS82_001604"
     CDS             1823..3274
                     /locus_tag="PWS82_001604"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL0906744.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3295..4131
                     /locus_tag="PWS82_001605"
     CDS             3295..4131
                     /locus_tag="PWS82_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0906745.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4131..4790
                     /locus_tag="PWS82_001606"
     CDS             4131..4790
                     /locus_tag="PWS82_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0906746.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4916..5605
                     /locus_tag="PWS82_001607"
     CDS             4916..5605
                     /locus_tag="PWS82_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906747.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5611..6567
                     /locus_tag="PWS82_001608"
     CDS             5611..6567
                     /locus_tag="PWS82_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL0906748.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6611..7123
                     /locus_tag="PWS82_001609"
     CDS             6611..7123
                     /locus_tag="PWS82_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906749.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7379..7927)
                     /locus_tag="PWS82_001610"
     CDS             complement(7379..7927)
                     /locus_tag="PWS82_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0906750.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8016..9197)
                     /locus_tag="PWS82_001611"
     CDS             complement(8016..9197)
                     /locus_tag="PWS82_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0906751.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9433..10941)
                     /locus_tag="PWS82_001612"
     CDS             complement(9433..10941)
                     /locus_tag="PWS82_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL0906752.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11100..11291
                     /locus_tag="PWS82_001613"
     CDS             11100..11291
                     /locus_tag="PWS82_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906753.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11494..12411
                     /locus_tag="PWS82_001614"
     CDS             11494..12411
                     /locus_tag="PWS82_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL0906754.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12404..13477
                     /locus_tag="PWS82_001615"
     CDS             12404..13477
                     /locus_tag="PWS82_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906755.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13625..14143
                     /locus_tag="PWS82_001616"
     CDS             13625..14143
                     /locus_tag="PWS82_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906756.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14124..14516
                     /locus_tag="PWS82_001617"
     CDS             14124..14516
                     /locus_tag="PWS82_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL0906757.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14592..15527
                     /locus_tag="PWS82_001618"
     CDS             14592..15527
                     /locus_tag="PWS82_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL0906758.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15660..16439
                     /locus_tag="PWS82_001619"
     CDS             15660..16439
                     /locus_tag="PWS82_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0906759.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16432..16749
                     /locus_tag="PWS82_001620"
     CDS             16432..16749
                     /locus_tag="PWS82_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0906760.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16993..18603
                     /locus_tag="PWS82_001621"
     CDS             16993..18603
                     /locus_tag="PWS82_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0906761.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18760..19608
                     /locus_tag="PWS82_001622"
     CDS             18760..19608
                     /locus_tag="PWS82_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0906762.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6343 a         3514 c         3955 g         5827 t
ORIGIN      
        1 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
       61 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
      121 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
      181 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
      241 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
      301 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
      361 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
      421 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
      481 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
      541 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
      601 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
      661 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
      721 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
      781 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
      841 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
      901 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
      961 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
     1021 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
     1081 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
     1141 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
     1201 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
     1261 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
     1321 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
     1381 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
     1441 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
     1501 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
     1561 gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
     1621 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
     1681 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
     1741 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
     1801 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
     1861 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
     1921 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
     1981 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
     2041 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
     2101 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
     2161 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
     2221 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
     2281 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
     2341 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
     2401 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
     2461 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
     2521 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
     2581 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
     2641 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
     2701 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
     2761 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
     2821 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
     2881 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
     2941 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
     3001 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
     3061 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
     3121 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
     3181 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
     3241 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
     3301 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
     3361 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
     3421 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
     3481 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
     3541 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
     3601 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
     3661 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
     3721 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
     3781 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
     3841 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
     3901 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
     3961 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
     4021 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
     4081 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
     4141 ccctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
     4201 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga
     4261 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
     4321 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc
     4381 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
     4441 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
     4501 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
     4561 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
     4621 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
     4681 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
     4741 aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa
     4801 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
     4861 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
     4921 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
     4981 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
     5041 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
     5101 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
     5161 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
     5221 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
     5281 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
     5341 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa
     5401 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
     5461 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
     5521 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
     5581 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
     5641 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
     5701 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
     5761 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
     5821 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
     5881 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
     5941 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
     6001 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
     6061 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
     6121 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
     6181 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
     6241 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
     6301 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
     6361 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
     6421 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
     6481 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
     6541 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
     6601 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
     6661 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
     6721 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
     6781 agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac
     6841 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
     6901 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
     6961 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
     7021 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
     7081 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
     7141 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
     7201 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
     7261 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
     7321 cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt
     7381 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
     7441 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
     7501 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
     7561 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
     7621 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
     7681 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
     7741 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
     7801 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
     7861 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
     7921 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
     7981 caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata
     8041 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
     8101 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
     8161 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
     8221 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
     8281 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
     8341 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
     8401 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
     8461 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
     8521 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
     8581 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
     8641 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
     8701 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
     8761 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
     8821 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
     8881 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
     8941 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
     9001 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
     9061 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
     9121 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
     9181 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
     9241 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
     9301 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
     9361 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
     9421 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
     9481 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
     9541 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
     9601 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
     9661 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
     9721 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
     9781 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
     9841 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
     9901 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
     9961 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    10021 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    10081 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    10141 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    10201 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    10261 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    10321 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    10381 caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    10441 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    10501 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    10561 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    10621 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    10681 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    10741 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    10801 aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt
    10861 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    10921 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    10981 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    11041 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    11101 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    11161 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    11221 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    11281 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    11341 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    11401 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    11461 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    11521 tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct
    11581 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    11641 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    11701 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    11761 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    11821 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    11881 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    11941 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    12001 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    12061 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    12121 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    12181 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    12241 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    12301 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    12361 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    12421 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    12481 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    12541 aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    12601 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    12661 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    12721 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    12781 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    12841 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    12901 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    12961 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    13021 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    13081 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    13141 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    13201 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    13261 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    13321 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    13381 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    13441 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    13501 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    13561 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    13621 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    13681 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    13741 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    13801 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    13861 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    13921 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    13981 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    14041 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    14101 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    14161 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    14221 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    14281 tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    14341 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    14401 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    14461 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    14521 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    14581 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    14641 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    14701 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    14761 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    14821 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    14881 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    14941 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    15001 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    15061 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    15121 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    15181 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    15241 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    15301 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    15361 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    15421 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    15481 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    15541 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    15601 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    15661 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    15721 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    15781 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    15841 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    15901 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    15961 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    16021 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    16081 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    16141 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    16201 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    16261 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    16321 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    16381 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    16441 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    16501 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    16561 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    16621 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    16681 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    16741 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    16801 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    16861 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    16921 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    16981 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    17041 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    17101 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    17161 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    17221 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    17281 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    17341 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    17401 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    17461 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    17521 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    17581 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    17641 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    17701 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    17761 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    17821 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    17881 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    17941 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    18001 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    18061 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    18121 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    18181 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    18241 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    18301 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    18361 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    18421 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    18481 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    18541 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    18601 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    18661 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    18721 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    18781 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    18841 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    18901 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    18961 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    19021 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    19081 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    19141 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    19201 atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta
    19261 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    19321 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    19381 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    19441 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    19501 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    19561 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    19621 aaaaaacata ccaaaaata
//
LOCUS       DANJJW010000051        19197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000051 DANJJW010000000
VERSION     DANJJW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            99..2540
                     /locus_tag="PWS82_001623"
     CDS             99..2540
                     /locus_tag="PWS82_001623"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL0906763.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2870..3574)
                     /locus_tag="PWS82_001624"
     CDS             complement(2870..3574)
                     /locus_tag="PWS82_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906764.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3761..4006)
                     /locus_tag="PWS82_001625"
     CDS             complement(3761..4006)
                     /locus_tag="PWS82_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906765.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4023..4874)
                     /locus_tag="PWS82_001626"
     CDS             complement(4023..4874)
                     /locus_tag="PWS82_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0906766.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5040..6044
                     /locus_tag="PWS82_001627"
     CDS             5040..6044
                     /locus_tag="PWS82_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL0906767.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6285..6641)
                     /locus_tag="PWS82_001628"
     CDS             complement(6285..6641)
                     /locus_tag="PWS82_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0906768.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7009..8196
                     /gene="tuf"
                     /locus_tag="PWS82_001629"
     CDS             7009..8196
                     /gene="tuf"
                     /locus_tag="PWS82_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0906769.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8384..10975)
                     /gene="mprF"
                     /locus_tag="PWS82_001630"
     CDS             complement(8384..10975)
                     /gene="mprF"
                     /locus_tag="PWS82_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0906770.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11001..11762)
                     /locus_tag="PWS82_001631"
     CDS             complement(11001..11762)
                     /locus_tag="PWS82_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0906771.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12040..12561
                     /locus_tag="PWS82_001632"
     CDS             12040..12561
                     /locus_tag="PWS82_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL0906772.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12620..13480
                     /locus_tag="PWS82_001633"
     CDS             12620..13480
                     /locus_tag="PWS82_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL0906773.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13665..15980
                     /locus_tag="PWS82_001634"
     CDS             13665..15980
                     /locus_tag="PWS82_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0906774.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16162..18003
                     /locus_tag="PWS82_001635"
     CDS             16162..18003
                     /locus_tag="PWS82_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0906775.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18027..19136
                     /gene="rpoD"
                     /locus_tag="PWS82_001636"
     CDS             18027..19136
                     /gene="rpoD"
                     /locus_tag="PWS82_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL0906776.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5551 t
ORIGIN      
        1 aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta tttgttttta
       61 cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc gttatgatag
      121 atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt actgcagttg
      181 aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa aaagataaaa
      241 tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat gttgccagac
      301 agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc aatgaagttt
      361 gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta ggtgaagcag
      421 aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg aaacagtatc
      481 agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac ggattgactg
      541 acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca caattttctg
      601 tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca gcacatgctg
      661 tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat gaagacgtgt
      721 tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc acgttacgta
      781 cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg aatgacggtg
      841 cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg ccttatttag
      901 caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga atcgctccaa
      961 tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca atcgatcttt
     1021 ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa ctgcagttag
     1081 atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca attggtgcaa
     1141 gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag aagagatacg
     1201 gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta gaagcgaata
     1261 tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac
     1321 ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg atttttcagg
     1381 aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg agtgaagtgg
     1441 aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa tggattccga
     1501 tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa
     1561 atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta tcaggaaaat
     1621 cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg attctttgtg
     1681 caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat atctctactc
     1741 gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg gatgtaaaag
     1801 atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac aagttacggg
     1861 agtggttccc tgaagaagag attctattca gtattttaag taattttgct acagaatcat
     1921 tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa gaaatcggtg
     1981 aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac ccttaccgtg
     2041 cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca gctacaggaa
     2101 atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat ggattatacc
     2161 aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta acggtgcctt
     2221 tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa gcatctttag
     2281 ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca gtagggttag
     2341 ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa ggccacatgg
     2401 gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa atcgagcaag
     2461 ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata caaatattgg
     2521 aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa gatttacagt
     2581 tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt attattagaa
     2641 ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat atcttcaggt
     2701 agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag gtcaagctga
     2761 tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt attatatgac
     2821 taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt tatcgatcag
     2881 taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg actttgtatt
     2941 tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa atagttgcta
     3001 atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca ttttgttctg
     3061 tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat
     3121 tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc gttttttctt
     3181 ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca gttggttcat
     3241 cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga ttgtcacgga
     3301 atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta ttgaagttgt
     3361 catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat ccgatcgtta
     3421 agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc
     3481 tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa ggacggaatt
     3541 gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc tcataccctt
     3601 tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac
     3661 gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg
     3721 gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc ggtatcaagg
     3781 caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt ttgaccatta
     3841 gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc cagtttcttg
     3901 caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct
     3961 gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa tcacctttcg
     4021 acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc taactgatcg
     4081 atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt ttctttgatt
     4141 ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga ccaacgcaaa
     4201 gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc aggcacatcg
     4261 aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg tttttttgca
     4321 tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt gaccatcggc
     4381 ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt gctcacaccg
     4441 attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt atctgcattt
     4501 gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa aaacagatct
     4561 aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac aagttcatag
     4621 ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg aattccactt
     4681 tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt atcgatatgg
     4741 cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc atcaggtgtt
     4801 tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg ataagtgctt
     4861 gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt gtagcgcatt
     4921 tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga
     4981 ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg aatgagaaaa
     5041 tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact ggttctccag
     5101 tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt cttcaaaaga
     5161 agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa gaaagcatga
     5221 tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc
     5281 agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat gtatttgttg
     5341 tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat tatcgtgagg
     5401 cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg attttgaatc
     5461 gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg aatttgactg
     5521 attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt
     5581 tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat gtaaaagtga
     5641 tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt gatatcatgg
     5701 ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg gatcatgaga
     5761 tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt aaagaagaag
     5821 tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg
     5881 agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg atcgtaccag
     5941 tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat ggtacgatat
     6001 ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga ctagaatatt
     6061 aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga tcggaaaaga
     6121 cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta
     6181 aacacgtata aacggcggaa gcagaagcaa ctccttctta tttcttggag ttaaacgctt
     6241 ctgtcccagc ctctttttat ctaataaaat tttaagacca tttactaatt atcgattttt
     6301 tctgccgcat ttttaggcac ttcagaagcc tttatttctt cccagcttaa tacaccttta
     6361 cgaggattga ctttgagttt aagatacgca ttcatcttta atggatgttc tctttcttcg
     6421 cgaagcgtca cctgtttttt ttcaccatct ttattataag ccgcttgctt gtaggtataa
     6481 gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca tttcgccatt cgtcgtgatt
     6541 tttgtataat aagcttcgcc gccgtaaaaa taatgataag ctgcaaaact tccgccgaga
     6601 aatatgccta aaacgacaag tacgccaatt aattttttca tgtacttcac ctctttcctt
     6661 attattaacg aaatagaaga tttcgtctat tgaaagttgt tggcaacagc tgaattttaa
     6721 acttttcctc attttgttct tattgcttca aaagaaagtt aaggactatc ttgaagtttt
     6781 tctcaaaaaa cacatttttt tatttataaa aggtactttt ttcgcgtata agacagacga
     6841 gtgctcttag ttgactttga ccaatattga cgtataataa gcttagttcg aatcttacaa
     6901 aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa agactataat aaaaaggtga
     6961 gagctgaaat ttttcagtga tctatattat ttaggaggtt tgtaaattat ggcaaaagaa
     7021 cattatgaca gaagtaaacc acacgttaac attggtacga ttggacacgt tgaccatggg
     7081 aaaactactt taactgctgc gatcactaca gtattaggca aaaaaggcct agcaaatcct
     7141 caagactatg ctagtattga tgcagctcca gaagaacgtg aacgtgggat tacgatcaat
     7201 accgcacacg tagaatatga aacagaaaaa cgccactacg cgcatattga tgcaccagga
     7261 cacgcggact atgtgaaaaa catgattaca ggggcagcac aaatggatgg tgcaatctta
     7321 gttgtttctg caactgacgg tccgatgcct caaacacgtg aacacatttt attgtcacgc
     7381 caagttggtg taaaatacct gattgttttc ttgaacaaag ttgatttagt cgatgatgaa
     7441 gaattgatcg atttggtaga aatggaagtt cgcgagttat tgagcgaata tggtttccca
     7501 ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag cattgcaagg cgatccagat
     7561 gctgaagctg ctattatgga attgatggat acagtagatg aatatatccc aacaccagaa
     7621 cgtgatacag ataaaccatt actattgcca gtggaagacg tcttctcaat tacaggtcga
     7681 ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc gtgtcggtga tgaggtagag
     7741 atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa caggtgtaga aatgttccgt
     7801 aaaacgttag attacgggga agctggggat aacgtaggcg tgttgttacg ggggatccaa
     7861 cgtgacgata tcgaacgtgg acaagtactt gctaaaccag gttccattac tccacataca
     7921 aaattcaaag cagaagtgta cgtgttgaca aaagaagaag gtggacgtca tactccattc
     7981 ttcaacaact atcgtccaca gttctacttc cgcacaactg atgttacagg aacaatcaca
     8041 ttgccagaag atacagaaat ggtcatgcct ggtgacaacg tgacgattga tgttgacttg
     8101 atccatccaa tcgctgttga aaacggaacg acattctcaa ttcgtgaagg cggacggaca
     8161 gtaggttcag gtatcgttac agagatcgaa gcataagaaa cgcgactcga atattttggt
     8221 aatgaaaaat gtttaaaata aaagagcggt ccaaacagga tttctgtttg gaccgctctt
     8281 tgttcttcaa ttgtataaga agagattgta gaagcatgaa ctttcgtttt acttcagtct
     8341 cttttattca tcatgagagt gttcatctat cggtatgcag tttttaatac tttcttcgta
     8401 tccatttttt caaaccactt attctacctt gtgtctgctc aactggcgca ttatcaataa
     8461 tcagcaaagc aatcataaca tatgcaatcc aactatctct agaatataga gtataacgtg
     8521 agacccagtt tgtagcgtac ttgcttttat attctcttaa tccatggaaa gaataaaaat
     8581 gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg taaaaaactc tttctggaag
     8641 ttccgacatt tgaaagaggg gccatcccta aattgaacca ttggatatgt tcttctttca
     8701 tataggcaaa caaatgcaag aacaagaaat ccatactgcc agaaggtgct tttttagaat
     8761 catatcgcat cagatcaatc gttcctactt tattgttcgt gtaagtaggc atgatcgttg
     8821 caaaagcgac aatttcttct tgggtatttt tgacaactgc aataggcgct ctttgtagat
     8881 aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt tcgtgaaccc aaccaattat
     8941 cagagatatt tttgaaagta ctcatttgct cagtagaaaa tggtggttgt aagacatcaa
     9001 atgtaaatcc ttctcgttcg atccgattca agacagcacg agtgcccttc atttttttcc
     9061 ctgaagttgt aaaactattc aggtctacat aagcttcttc ccccatctta atgaaatcgt
     9121 agccgaattc atgcaaaatc ataacaatct cttcgcttgt ttcgtagaaa actggtaaat
     9181 agcatagtcg atctgtttct tcgataaaag cttcgattgc ttctggaaaa tcttctttct
     9241 taccagaagg atcacccatt acaatacatt tattattgaa gcaagcgaat tggagaagaa
     9301 cagttggttc accgtctttt tcataggcaa acatccgctt atcttttaag aagatcaatt
     9361 gactatcgga gtttcctcca taagttgtaa ggatcttcag ggccttttct tcattaaagg
     9421 cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac aaatagaacg atcatgaaac
     9481 taacagcgat gatggccaac aatcccgaaa accaaatctt ttcagaagga aataagaaaa
     9541 aggaaataaa atggtgacgc cgatgaggga aatccggcag attgtatacc ccaataacga
     9601 tgtaaagcaa ggacagtgca ccaataatga ttccatcgat cgtacgccac tcccatgaat
     9661 agatcaattg ttcccgaaat aattcgtttt tagaagcaag aatgatcaga agcagaatag
     9721 acaagaaaac caccggtgtg aaactaaaat cacttaataa aacataaagt aaagctagaa
     9781 caatcaagaa gattgtaggc aaatatgctc gtttcacacg tgctgcgatc cctctgccca
     9841 taatgattaa aagaaatccc aatagtatac taggaaattg gataataaaa tgaaatttca
     9901 atgggttcaa actatgaagc cagcgaaact ctgtaaaagc ttgcgggata gtggcagaaa
     9961 gtactagcat gattccagaa aagtataata agacaaccac gattttatgc gcaatttctg
    10021 ttgctaattg tttagggata ccagaataac gctgatcaaa agtggaaccg atatttttga
    10081 agaaaaagac gattccaatc aaaaacggaa tgatgtagta gaacaggcga tagagcaaga
    10141 tccacacaac gactatttct cttggcacac caatagcgga caacccgata atcatcatta
    10201 catcgaaact cccaatttct cctggaatca tagacacgat tcctatcact gaagcagcaa
    10261 tgaataatgg aattgtttgc cacagatcaa tccggatttc cattaaataa ccaacagaaa
    10321 ggaaactaaa caggacaccg gtccattcca acaatgaaac tagcaatagc tgtccttgag
    10381 ttttaagtga ggtaccacca aggaatcctt tctttttgaa agtagtaaat aaaaagacga
    10441 caggaaaata tagaccgccg ccgatcaacc atatccaata ttgcttcaaa aaaggagcaa
    10501 ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta aatagaaagt ccagccatca
    10561 aaaacaagaa aactttcgat aatgcttgga tcacgtttct gctctctttc ttttgtccat
    10621 atagttcaga gcgaagcccc acactaacaa acccgccgaa accggcaata ttgttgatcg
    10681 tattgatcag ccagcttgtc tcaaacaggt aacgaggatt ctgcttttgt tcaagcatcc
    10741 gattcaaaat aatatcataa ccaaccatag gtaacacaga gataagacca atcaatagca
    10801 ttagtccgct tttccataca ggtatagtgg caaacgtttc tcctaattgc tggatagata
    10861 gagttttacc gatggacatc aattcaccga caataatgac aatgacagaa atcaaaaaga
    10921 ttgttttgaa cagtcctagg tggtttttca gccattgtat ggtatttttt aacaaataaa
    10981 tcaccttcta ttcctaatag ttatgtttca gaaagaaatt gaagaacttc tcgattgaag
    11041 cgttcaggat cttttctagc taattcatgt ccttgttctt tcacaagcac aaaggaagct
    11101 tttggtatag ttttagctat atacaaagaa tgttttagct tgattacatc tttttttccc
    11161 acgatgatca aagtggggga gttgattttt tttaaatcat tttctgtaag cccgatgtca
    11221 tgcagcaaaa gtttgataac taaaagattt ttgcgaagac ttgggcgaaa taaagagagt
    11281 aaccataccc atgcataatg aatattactg atgactctgg cagaaaaacg aacgccttta
    11341 acaagcgtgt ttccagaatt taaaatcaat cgatgaactt ttttagggaa gctgctggca
    11401 aataccagtg ctagatttgc tccatcacta aatccaagaa aatctgcctg atcgattttt
    11461 tccagcaaca tgatcgtatt cagatcctcg gccattaact gaaaattcaa catagatgct
    11521 tcgtttgtcg aacgaccatg tcctcggcta tctactagat acacggtata gtagcgttcg
    11581 agtacaggga cctgttctga aaaaaagcgg ccgctgccgt cattgccatg cagtaaaaag
    11641 agagggaagc cttgaccgct cttttcatag taaatttcgc tcccatctgg catttttgca
    11701 tatttctttt cttttttcac ataatcacct actgtgtaaa gtataacgat tatatcagtc
    11761 attgggcaat tgtttttact gaaaaacaac gatcattttc gtaagatgtc aaaaaataca
    11821 ggaaaattca ttttttctta aggcgtacac agaaatcaag gaagaaatag ccaagaagat
    11881 aaagaaatag taagataaaa aaagatgtca aaataactag gtgatgaaat gaagcgaaaa
    11941 cgattatcta ttatccgtat tcttttttac atgatcattc tgacggttat tggtgcggtg
    12001 ttgtttttat gaggaagaac ttatcgttcg gtaaaacgag tggaaagcta tcaaatagaa
    12061 ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg agaatttgat caaggcgata
    12121 atattgaccg aatcaaaagg aaaagggaca gatctcatgc agagctcgga aagtctgcat
    12181 ggagaagctg agatcattga taatccagct gacagtattg atcaaggtgt caaatatttg
    12241 gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt tatggacagc tgtccaagca
    12301 tataattttg ggaaagatta tattgcctat gtaaaaaatc atggcggaga aaacacagta
    12361 gaattagcag aagcgtattc taaagatgtg ctgagcccac ttttaggaaa tgaggaaggg
    12421 aaacaatacc gatattggcg gatccagtcc ttgctttaca atggcggcta tctctatcat
    12481 aatggaggta acatgtttta tcctgatatt gtcaaaatga atgaaaaaat aatagagacc
    12541 tatgaaaaag tctttaagta aaatagcagg aaatgttcaa tcatgaataa aattaaatga
    12601 aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg gaaaatcaag aagtggggta
    12661 cacaaccaaa gactggatgt tacggtttgt gaagggaatg tttatcggtt caggatttat
    12721 cttgccagga gtcagcggtg gtgcattagc agctgtcttt ggcatgtatg agcggctgat
    12781 ttctttttta gcccacctta caaaaaattt caaagaaaat gttttgtttt ttctgcctgt
    12841 tggtctaggc ggagtgacag gcatctttct actttctttt ttagtcagtt ttttattagg
    12901 tgcgtatgaa acaacgatat tatggttttt tgtcggatgt atcgctggaa ctgttcctgc
    12961 gttatggaaa gaagcaggga aaaaaggaag agatacttct gatgtgatca tcatggttgt
    13021 tacttttgtt ctggcttatt tgtttttatt atacggcgct caattatttg acgggcaagt
    13081 cgatcagaat tttggaacat ggctgattgc cggcggattg attggtttgg gtatgatcgt
    13141 cccaggattg agcccttcta attttttggt gtatatggga ttgtataaag cgatggctga
    13201 cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc ggaattggtg ggattctttg
    13261 tgttcttgga ttgtcaaaaa tcatggatta cattttctca aaggcatata gcaagctatt
    13321 ccatttcatt ttaggtgtag tttttgcttc aacgattatg atcattccta ccgattacgc
    13381 gggattggga gttgctgggg tactagcttg tcctgttctt tttatagccg gtacagcact
    13441 aggttggtgg atgagccgtt tggaggaaca atataagtaa agatagattg ataataaaaa
    13501 cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt ttgtaaaatc attcgtgtgt
    13561 ttttctataa attttataaa aacaagttgt ttttaatctt ggaacacgct agacttaaac
    13621 acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa gaaaatgaat ggacttacac
    13681 agcaagaggt agttcagaaa atggcttctg gccagcaaaa tgactaccag gaagatgcat
    13741 caaaaagtac aaaacaaata ttcagcgata atatattgac gttattcaat tttctgaact
    13801 ttggtattgg gatttgtctg ttatttgtag gtgcgtattc caacatggcg tatttagcaa
    13861 ttattgtcgt caatatcgtt attggaattt atcaagaaat acatgcaaga aatcttgtac
    13921 ggaaattatc gattgtttcc caatcagaag tactagtgat ccgggacggt aaagaacaat
    13981 cgatttctac taaagagctg gtattaggag atttggtaaa aatcggagca ggagaacaga
    14041 taccagccga tttacaggtt atatctggac gggcagaagc aaatgaagct cttttgacag
    14101 gcgaatctga tttgattgtc aaagaaactg gagctgaatt gctttctgga agctttttga
    14161 caagcggttc tgtcatggct gaagttatcc gagtaggtgc agataattat gctgtccgct
    14221 tgactagcga ggcaaagact cacaaagcta ttcgttctga acttgtagat tcgattcgca
    14281 aagtatccaa attcactagt ttcgtgatta ttcctttggg gatcatacta tttttggaag
    14341 cactgttcat ccgcgatgct tctatcaaaa tttctgttat tgcatctgca gcagctttgt
    14401 taggcatgct tccaaagggt ttggtattgc taataagtat tgcattaacg acaggggtaa
    14461 ccaaactggc aaaaaaacga atccttgttc aagacatgta ttcaattgaa acgctggccc
    14521 atgtcgatac attatgcttg gataaaacag ggaccattac tgaagggaaa atgagtatcc
    14581 agcgggtaga agcactacat ccgctgtacg agaaagccat cccagaaatt atggggacgt
    14641 atctggcaga aagcagtgat aacaacatca ctatgcgggc tatgagagat tattttgagc
    14701 tttctaatcg gtatgatgtg acaaacaaag tcgcattttc ttctgatcgc aaatggggcg
    14761 cgatggaatt tccagaactg ggagtcgtgt acgtaggagc gcctgaacgt ttggtggaag
    14821 aagatcggct tccggacagt ctaaaaacag cccaagcgaa tggctatcgc gtacttatgt
    14881 tggggatcgc tccaaatcag caattgaacg aagaaaaacc agcatttgtt gagccggttg
    14941 ctctgtttga aatcgatgat ccaatccgaa aaaacgctca agaaacacta gcttatcttc
    15001 atgaggaagg tgtggatctg aaagtgattt ctggagacaa tccttttact gtctccaata
    15061 tcgctagacg tgcaggatta ccgaattatg atgcgtatgt cgatctttct caaattactg
    15121 aagaaatgga agtgcgagaa gctgctcatc gctatacggt ttttggacga gtgagtccgc
    15181 agcaaaagaa attgctggta aatgaattga aagaaagcgg cagaacggtt gcaatgacag
    15241 gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga ttgcagcatt gctatggcag
    15301 aaggtgatgg agcaacacgt cagattgcga atctggtatt gttggactct gattttacta
    15361 cattgccaga agtcctgttt gaaggacgcc gagtggtcaa caatgtgacg aaagtctcag
    15421 gcatcttttt tatcaaaacg atttattcat ttattttatc gattatatgt gccgttacag
    15481 caattggatt tccgtttatc cctatccaaa tcacgctgat tgatttagcg attgaagggt
    15541 atccttcctt ctttttatct tttgaacaag ataaaagaaa aatcacttac cgttatttgc
    15601 ctacagcact tattaatgct ttaccaaatg ccttgcttgt agtagtgaat atcattgcgg
    15661 tgtatctact tggaaaaaca ttcgcatggg gaaatgctga aactactact ttgatgtatt
    15721 acttgctgat tgggatcagt tgcatggcag ttgtaaaagc ttgttatccc ttcaacccat
    15781 tgcgcatctt cctagcagta acgacgatcg tagggatata tgtagctgca atgctgttcc
    15841 atggaatttt ggaagtaaac ttagggttgg gagagagcgt aggatacttc tttgtattca
    15901 tgctcgtaaa tattgttctt cgagtattgt actggaaggc tgatattcaa aatcagttac
    15961 tgaaaagagt agcaaattga tgcttaatga gatagaaatg tgttatactt gaaaaagcaa
    16021 tgaaatatga tacaatcaaa catgtataat tgtgtccttt aaaatcgcac tgatttcagt
    16081 tagtgcgatt ttttaccggt tattctcctg aaatatcttc tgttaaaccc aaaagatatc
    16141 aatagagcag ggggtgaaaa aatggctcaa cggattcctc aagaggttat cgaaacagta
    16201 cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg tccagttgaa aaagtctggt
    16261 aaaaattact tggggctgtg tccttttcat gaagaacgta caccgtcttt ttctgttgca
    16321 gaagacaagc agatttttca ttgctttggt tgtggtaaag gcggaaacgt gtttactttt
    16381 cttcaagaac tagaaggtct aagttttcca gaagcagtgg taaaagtggc agacttcgaa
    16441 cagataccgt tagaagatca atggcgtcag cgtactatag ctgtccaaaa tcctgaatct
    16501 gctacgggga aactgattgc tgctcatgaa aaagcagcgg aaatctatca tcacatgcta
    16561 ttgcatacaa aagctggaca gccagcttta gaatatttgc aaagtcgtgg gttgtcttta
    16621 gaattaattg aagaattcaa cataggtttt gcacctaatg agcgttcttt tcttcagcaa
    16681 gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac gctctggatt gtttgtcacg
    16741 catgatgatg gccgtatgtc tgatcgtttt tatcaaagag tgatgtttcc acttcgtaat
    16801 tctcaaggcc agacgatcgg tttttcagga cgctggcttg cacctgaaaa agggcaggaa
    16861 aaagatcagc ccaaatactt gaatagtcca gaaacagaac tgttcaataa aagacaagtt
    16921 ttatttaatc ttgataaagc aagaagcgat atccggaaaa acggagaaat tttgttgttc
    16981 gaagggttta tggatgtgat tgctgcatgg caggctggta tcaaaactgg attagcttct
    17041 atggggacaa gtctaacagc tcaacagatc caacaaatgg aaaagctgac gaaagaattg
    17101 gtcttttgct atgacggaga caacgcaggt tttgaagcaa ccaatcgagg gattgaattg
    17161 cttcggcaaa acagtcgatt agcactttct gtcgtagtcg ttccagaaaa gcttgatcct
    17221 gatgaatatc tccgtaagta tggagaagca tctttccaag aattagtggc tcatgggcgc
    17281 gaaacagtct ttacttttaa atcacgttat ttaaaacaag ggaaaaattt agataatgaa
    17341 aaagacaaga tcgaatacct cgaagaatta ctagttgaac ttagccgagt tgtttcacca
    17401 atcgaacaag atatgtatct atctcaactg tcaacagagt ttcagatcgg cagagagacg
    17461 atacaaaaac aactgcgcga tcttcgaagg acaaatcagc aaaatcgtca agaacctcag
    17521 gaaagtcaac acactcaggt tacgagacaa gaaactgcac ggaaaaagag acctttgacg
    17581 caagtagaaa aggcagagca gattctatta tatcgtgcat ttaaagaagt ttctgtccgt
    17641 aatattttaa aagaacgaga atttcagttt attcatgatg catatgcaga agtttatttt
    17701 ttattcgatg catatgtctc acaacattat gaattcaacc ttgcagagtt tttagacttt
    17761 ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg aatatttacc ggtggcagaa
    17821 gaaagttcgc cacgagaaat ccaagacttg ctagatgtga tcagcaaatc tggcttagct
    17881 gatgaaataa cattaaaaaa acaaatgcag caagaagcca gacgaactgg aaacaaacag
    17941 cttgagcttg aattgacgat tgaaatcatc aatttaacga aacagttaaa acaagccaaa
    18001 tagctactca aggggggcct tcttttatgg caaatggaac agataaaaaa acgtatgaag
    18061 cagcagtagc agctttcatc aaagaaaaca aacccaaacg acaagttgta tatgatgatt
    18121 tatccaatca gcttgctaca ccattcactt tgaatgcaga tgaaatggac aaattgatcc
    18181 aaaagattga agacgcaggt atcagtgtgg tggatgaaaa tggtgatcca tcaattcaca
    18241 gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac agaagatctt tctgcgccaa
    18301 ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa agaaattggc cgtgtctccc
    18361 ttttaactgc ggaagaagaa gtagcgttag cattgaaaat cgaagaaggc gaccaagaag
    18421 cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt aagtatcgct aaacgttatg
    18481 taggacgagg catgcagttc cttgacttga tccaagaagg aaacatggga ctgatgaagg
    18541 cagttgaaaa atttgactac cgcaaaggat ttaagttttc aacctatgct acatggtgga
    18601 ttcgtcaagc catcactcga gcaatcgctg accaagcacg tacgatccgt attccagttc
    18661 atatggttga aacaattaat aaattgatac gtatccaacg tcaattgctg caagaccttg
    18721 gaagagaacc tacgccagaa gaaatcggtg cggaaatgga tcttccaaca gaaaaagtcc
    18781 gagaaatctt gaaaatcgcg caagaacctg tttcccttga aacgccaatc ggtgaagaag
    18841 atgactctca cttaggtgat ttcatagaag accaggaagc aacaagtcca gcagaacacg
    18901 cagcttatga gttactaaaa gaacaattgg aagatgtact agatacattg actgatcggg
    18961 aagagaatgt ccttcgctta cgttttggtt tagatgatgg ccggacgaga acattggaag
    19021 aagtagggaa agtatttggt gttactcggg aacgtattcg tcagatcgag gctaaagcat
    19081 taagaaaatt gcgccatcct tctcgttcaa aacaattaaa agatttctta gaataagcgt
    19141 ggactattga ccagaagaga aatcagtaaa actgtttggt ttattttaaa agaaaaa
//
LOCUS       DANJJW010000052        19139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000052 DANJJW010000000
VERSION     DANJJW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(154..1722)
                     /locus_tag="PWS82_001637"
     CDS             complement(154..1722)
                     /locus_tag="PWS82_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0906777.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1726..2007)
                     /locus_tag="PWS82_001638"
     CDS             complement(1726..2007)
                     /locus_tag="PWS82_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0906778.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(2004..2927)
                     /locus_tag="PWS82_001639"
     CDS             complement(2004..2927)
                     /locus_tag="PWS82_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL0906779.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2924..3802)
                     /locus_tag="PWS82_001640"
     CDS             complement(2924..3802)
                     /locus_tag="PWS82_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL0906780.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3789..5126)
                     /locus_tag="PWS82_001641"
     CDS             complement(3789..5126)
                     /locus_tag="PWS82_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0906781.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5129..6232)
                     /locus_tag="PWS82_001642"
     CDS             complement(5129..6232)
                     /locus_tag="PWS82_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0906782.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6306..7814)
                     /locus_tag="PWS82_001643"
     CDS             complement(6306..7814)
                     /locus_tag="PWS82_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0906783.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7780..8472)
                     /locus_tag="PWS82_001644"
     CDS             complement(7780..8472)
                     /locus_tag="PWS82_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0906784.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8494..9468)
                     /locus_tag="PWS82_001645"
     CDS             complement(8494..9468)
                     /locus_tag="PWS82_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906785.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9768..10640
                     /locus_tag="PWS82_001646"
     CDS             9768..10640
                     /locus_tag="PWS82_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906786.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11222..13420)
                     /locus_tag="PWS82_001647"
     CDS             complement(11222..13420)
                     /locus_tag="PWS82_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906787.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13652..14644
                     /locus_tag="PWS82_001648"
     CDS             13652..14644
                     /locus_tag="PWS82_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906788.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14704..16125
                     /locus_tag="PWS82_001649"
     CDS             14704..16125
                     /locus_tag="PWS82_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL0906789.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16118..17617
                     /locus_tag="PWS82_001650"
     CDS             16118..17617
                     /locus_tag="PWS82_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL0906790.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17656..19050
                     /gene="uxaC"
                     /locus_tag="PWS82_001651"
     CDS             17656..19050
                     /gene="uxaC"
                     /locus_tag="PWS82_001651"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0906791.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5769 a         3892 c         3373 g         6105 t
ORIGIN      
        1 attgcattat cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat
       61 tgataacttt ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat
      121 aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt
      181 gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc
      241 gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt
      301 cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt
      361 gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac
      421 tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata
      481 acaataaata taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt
      541 attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc
      601 taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc
      661 tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt
      721 cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc
      781 ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa
      841 ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc
      901 accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac
      961 taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat
     1021 aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac
     1081 tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa
     1141 atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg
     1201 accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt
     1261 cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag
     1321 atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg
     1381 acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc
     1441 acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc
     1501 ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt
     1561 tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc
     1621 aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag gatctggatt
     1681 gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct
     1741 cattcttact cgatataaac tgaatgcccc agctgctaaa aaagcaagtg aaataaacaa
     1801 cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt ctaaagacaa
     1861 agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg cagacatatt
     1921 acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac cgaccattcc
     1981 tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa atgtatccat
     2041 tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt gacagtctgt
     2101 tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc acctatctta
     2161 cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata gacgtttcta
     2221 gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt ttgatttttc
     2281 ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg gatatgacac
     2341 tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc aatccagcaa
     2401 tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca aaaaggagta
     2461 ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata accagatagt
     2521 cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc ctgtcctacc
     2581 ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac attcgatcct
     2641 tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag atactcctgt
     2701 tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt aaaattggat
     2761 aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac ctttggatgg
     2821 ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa atccgtcggg
     2881 gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa tgctccctta
     2941 tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct gttaaagaaa
     3001 aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc ccttttctca
     3061 gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag ctaagacgag
     3121 ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat agatttggca
     3181 catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtgtt tcgatgtcca
     3241 cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc acccaccagc
     3301 ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc
     3361 ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc cataacgttt
     3421 ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg cgttcttcaa
     3481 atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact tctgcaaaag
     3541 atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact gaaaaatctc
     3601 cgccgtgaga atgcaggtaa aggaccgtcg gaaacggtcc ttctttttct ggataagcat
     3661 agagggctgg tactgtttcc aattcattta agtacaattg atagtattca agcataaaac
     3721 cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta gatggagcac
     3781 ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc atccatattt
     3841 tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc taatagaaat
     3901 gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc cgcaaagcca
     3961 atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg aacaccggga
     4021 actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg tatgaattgt
     4081 tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt gatattcgaa
     4141 aaatggatcc ttctgaatgc tgatgtccgt tcatctatcg gataagcctt tttttcccat
     4201 acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat aaatggacac
     4261 atcacattat ccatgactat attggatact cgaatatctt caacgatccc gcctcgacct
     4321 cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt tgaaatggta
     4381 atattacgaa tactaccact catttcactt cctaatacga caccgccatg tccatggacc
     4441 atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt atcttcagtc
     4501 cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact aatacgcaca
     4561 tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg gttcaagatc
     4621 gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca acttggtgac
     4681 tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact catcaattta
     4741 ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca tttttttcca
     4801 tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg agcatagata
     4861 caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac aggataatct
     4921 tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc gacattgctt
     4981 tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt cccgcctccg
     5041 tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc atcaattgat
     5101 gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc aatgcttgca
     5161 agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg tagtaagcat
     5221 atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt ttattcaagt
     5281 tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga tgtgatttct
     5341 tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct ttagccattg
     5401 cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt tttgtttctg
     5461 ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat gcagccagtg
     5521 tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt aattcatagg
     5581 tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga tgggatagac
     5641 ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga atcagcaaat
     5701 ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa acatcttggt
     5761 aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa cccatatata
     5821 agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct ccttcggatg
     5881 tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta tatttttctt
     5941 cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg tcgatattgt
     6001 attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag tcgatattgt
     6061 cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat tggaaccctt
     6121 taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct tcatataagc
     6181 gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc attatttgct
     6241 ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg tcccaatcgt
     6301 ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct
     6361 ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag ctgtcccatg
     6421 atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac ttcattgttc
     6481 gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt ttctttgatc
     6541 aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt cacttcacta
     6601 ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc gataaattcg
     6661 atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt catcaattta
     6721 tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa ttgtgcttcg
     6781 tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc ttctgaccaa
     6841 acatgataac cgccgccttc accatctgag atcttgttta caggcactaa ttccatatca
     6901 tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc agtgtagatt
     6961 ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct
     7021 tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt catatactct
     7081 tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc atttggtgct
     7141 ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg cgcacgatcg
     7201 ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt aaattgttta
     7261 gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt atccaaccag
     7321 tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac gccgtacaag
     7381 gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa atttttatag
     7441 tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt gcggacagaa
     7501 gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc agaagccaat
     7561 gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt cagttccgta
     7621 tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa
     7681 atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact gttacttgaa
     7741 ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa ttttacgagc
     7801 gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt tttcttctgt
     7861 tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg ctgtccacgt
     7921 gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca ttgtttggat
     7981 gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat gaagatgtga
     8041 gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat cataatgagc
     8101 catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa tatacaaagc
     8161 gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa tcccgatggc
     8221 gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat atgtgaagta
     8281 cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca ctgcaaccat
     8341 cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta aaccgcccca
     8401 cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta ttttatctaa
     8461 tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt ctcctagctt
     8521 tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt taaacaatcc
     8581 cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa tataggtgtc
     8641 gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat gctcgaaccc
     8701 taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg gacgaattgc
     8761 cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc
     8821 atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag accagcccaa
     8881 ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg atgttagaaa
     8941 actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc cgtcaacatt
     9001 caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag ggatgtaaat
     9061 gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata gtaaagacaa
     9121 aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca acgtatttct
     9181 taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga aaccgaccca
     9241 aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa cgagtccccc
     9301 cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca tcacatataa
     9361 aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct tttcttcttt
     9421 tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa gcccctcctt
     9481 agttttgtga atgtttgctg cttatgactt catgatagat aactatctga attacgtata
     9541 taatcatttt tatcgtaagt aaaaacgctt acatagcaat gatttttgtg ctatacaaaa
     9601 cgataaagtg tattttttca caaataaact acgcatagca aaccttctat ttttttctat
     9661 ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat aaattgaaca
     9721 tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg aaaaagaaag
     9781 tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt ttgctagctt
     9841 tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct acacaagaag
     9901 tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg tatcgctaca
     9961 ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt acaggtgtcg
    10021 gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc agaaagtatt
    10081 tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc agcgggggga
    10141 tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac tgggccatga
    10201 tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt ttccaagcct
    10261 tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt ggtgtatttt
    10321 tcaaaattat gctgccatta gctttaccgt ctattgctac tatctcgctt ttttatgctg
    10381 ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca tctaaatggc
    10441 cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta
    10501 gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc gttattgcaa
    10561 cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa ggagcactcg
    10621 taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg
    10681 tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt tgtgaaattt
    10741 cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga
    10801 cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta acccacagga
    10861 gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat gccttattgt
    10921 cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca agacttttgt
    10981 cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac aaatactagg
    11041 gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc tcttgattaa
    11101 acggcgttta tcttgttgat tctgcggcaa taaaatcagc cagcagcaaa acatgaagtg
    11161 ctgttttttg ctgtcctctt ttattgcttg aatcagatca caagaaaaac agcctcttga
    11221 ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac
    11281 gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt
    11341 tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa
    11401 atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc
    11461 tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa
    11521 tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg
    11581 gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg
    11641 cttttacata aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc
    11701 ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt
    11761 tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc
    11821 cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt
    11881 tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta
    11941 cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt
    12001 cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt
    12061 gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc
    12121 gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg
    12181 gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt
    12241 gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac
    12301 tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata
    12361 aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg
    12421 cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac
    12481 tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc
    12541 tgtccgaaaa taagtaggaa aaaagaccaa tgagtcctac cagtgttaaa gacactgcaa
    12601 ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc
    12661 tgtaaatcca attattataa tctgatttca gtactatata cgttttatct ttttctctta
    12721 tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat
    12781 tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt
    12841 ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg
    12901 catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct
    12961 tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct
    13021 cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta
    13081 aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt
    13141 cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt
    13201 tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag
    13261 ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat
    13321 aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca
    13381 tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt
    13441 aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc
    13501 ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat
    13561 cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa
    13621 caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc
    13681 agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa
    13741 tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag
    13801 tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca
    13861 taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga
    13921 cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct
    13981 gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga
    14041 ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc
    14101 ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat
    14161 tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa
    14221 agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatctagc
    14281 ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt
    14341 atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc
    14401 attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt
    14461 cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt
    14521 gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat
    14581 cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt
    14641 gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt
    14701 tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt
    14761 cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat
    14821 aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga
    14881 aatatgatga accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc
    14941 gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat
    15001 gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat
    15061 acaacagagg caggaatcca attcgtggag actgataatt taacagatac tcccccacag
    15121 agcttcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc
    15181 tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt
    15241 ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa
    15301 aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca
    15361 ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc
    15421 atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct
    15481 ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg
    15541 cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta
    15601 gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa
    15661 ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa
    15721 gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt
    15781 tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag
    15841 gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa
    15901 gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat
    15961 ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa
    16021 ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc
    16081 cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac
    16141 aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac
    16201 acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa
    16261 gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg
    16321 tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa
    16381 tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt
    16441 tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga
    16501 tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca
    16561 gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc
    16621 ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc
    16681 agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac
    16741 agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt
    16801 ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac
    16861 agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa
    16921 atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc
    16981 tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg
    17041 cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga
    17101 cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc
    17161 atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac
    17221 gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa
    17281 accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc
    17341 ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata
    17401 tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat
    17461 ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt
    17521 tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg
    17581 agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag
    17641 aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa
    17701 aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat
    17761 ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc
    17821 gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga
    17881 aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga
    17941 aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg
    18001 ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct
    18061 gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat
    18121 gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag
    18181 gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca
    18241 tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca agaactgata
    18301 gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac
    18361 gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa
    18421 aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatct tacagaaaca
    18481 ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat
    18541 gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat
    18601 ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa
    18661 acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt
    18721 ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga
    18781 tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt
    18841 agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact
    18901 agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga
    18961 gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat
    19021 gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa
    19081 acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaa
//
LOCUS       DANJJW010000053        18488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000053 DANJJW010000000
VERSION     DANJJW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            64..179
                     /gene="rrf"
                     /locus_tag="PWS82_001652"
     rRNA            64..179
                     /gene="rrf"
                     /locus_tag="PWS82_001652"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            193..265
                     /locus_tag="PWS82_001653"
     tRNA            193..265
                     /locus_tag="PWS82_001653"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Val,seq:tac)
     gene            269..341
                     /locus_tag="PWS82_001654"
     tRNA            269..341
                     /locus_tag="PWS82_001654"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Lys,seq:ttt)
     gene            349..430
                     /locus_tag="PWS82_001655"
     tRNA            349..430
                     /locus_tag="PWS82_001655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Leu,seq:tag)
     gene            448..520
                     /locus_tag="PWS82_001656"
     tRNA            448..520
                     /locus_tag="PWS82_001656"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Thr,seq:tgt)
     gene            532..603
                     /locus_tag="PWS82_001657"
     tRNA            532..603
                     /locus_tag="PWS82_001657"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:gcc)
     gene            620..705
                     /locus_tag="PWS82_001658"
     tRNA            620..705
                     /locus_tag="PWS82_001658"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:654..656,aa:Leu,seq:taa)
     gene            716..789
                     /locus_tag="PWS82_001659"
     tRNA            716..789
                     /locus_tag="PWS82_001659"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Arg,seq:acg)
     gene            799..872
                     /locus_tag="PWS82_001660"
     tRNA            799..872
                     /locus_tag="PWS82_001660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Pro,seq:tgg)
     gene            892..964
                     /locus_tag="PWS82_001661"
     tRNA            892..964
                     /locus_tag="PWS82_001661"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Ala,seq:tgc)
     gene            992..1065
                     /locus_tag="PWS82_001662"
     tRNA            992..1065
                     /locus_tag="PWS82_001662"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1026..1028,aa:Met,seq:cat)
     gene            1079..1152
                     /locus_tag="PWS82_001663"
     tRNA            1079..1152
                     /locus_tag="PWS82_001663"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1113..1115,aa:Ile,seq:cat)
     gene            1176..1265
                     /locus_tag="PWS82_001664"
     tRNA            1176..1265
                     /locus_tag="PWS82_001664"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1212..1214,aa:Ser,seq:tga)
     gene            1278..1351
                     /locus_tag="PWS82_001665"
     tRNA            1278..1351
                     /locus_tag="PWS82_001665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1312..1314,aa:Met,seq:cat)
     gene            1370..1445
                     /locus_tag="PWS82_001666"
     tRNA            1370..1445
                     /locus_tag="PWS82_001666"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1403..1405,aa:Asp,seq:gtc)
     gene            1447..1517
                     /locus_tag="PWS82_001667"
     tRNA            1447..1517
                     /locus_tag="PWS82_001667"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1479..1481,aa:Gly,seq:tcc)
     gene            1531..1604
                     /locus_tag="PWS82_001668"
     tRNA            1531..1604
                     /locus_tag="PWS82_001668"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1565..1567,aa:Ile,seq:gat)
     gene            1629..1716
                     /locus_tag="PWS82_001669"
     tRNA            1629..1716
                     /locus_tag="PWS82_001669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1663..1665,aa:Ser,seq:gct)
     gene            1738..1809
                     /locus_tag="PWS82_001670"
     tRNA            1738..1809
                     /locus_tag="PWS82_001670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1771..1773,aa:Glu,seq:ttc)
     gene            1832..1921
                     /locus_tag="PWS82_001671"
     tRNA            1832..1921
                     /locus_tag="PWS82_001671"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            1934..2007
                     /locus_tag="PWS82_001672"
     tRNA            1934..2007
                     /locus_tag="PWS82_001672"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1968..1970,aa:Met,seq:cat)
     gene            2026..2098
                     /locus_tag="PWS82_001673"
     tRNA            2026..2098
                     /locus_tag="PWS82_001673"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2059..2061,aa:Asp,seq:gtc)
     gene            2102..2174
                     /locus_tag="PWS82_001674"
     tRNA            2102..2174
                     /locus_tag="PWS82_001674"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2135..2137,aa:Phe,seq:gaa)
     gene            2179..2249
                     /locus_tag="PWS82_001675"
     tRNA            2179..2249
                     /locus_tag="PWS82_001675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2211..2213,aa:Gly,seq:tcc)
     gene            2262..2335
                     /locus_tag="PWS82_001676"
     tRNA            2262..2335
                     /locus_tag="PWS82_001676"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2296..2298,aa:Ile,seq:gat)
     gene            2347..2418
                     /locus_tag="PWS82_001677"
     tRNA            2347..2418
                     /locus_tag="PWS82_001677"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2380..2382,aa:Glu,seq:ttc)
     regulatory      2607..2686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2684..3877
                     /locus_tag="PWS82_001678"
     CDS             2684..3877
                     /locus_tag="PWS82_001678"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL0906792.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4007..5479
                     /locus_tag="PWS82_001679"
     CDS             4007..5479
                     /locus_tag="PWS82_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0906793.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5562..6350
                     /locus_tag="PWS82_001680"
     CDS             5562..6350
                     /locus_tag="PWS82_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL0906794.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6417..7085)
                     /locus_tag="PWS82_001681"
     CDS             complement(6417..7085)
                     /locus_tag="PWS82_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL0906795.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7200..8912
                     /locus_tag="PWS82_001682"
     CDS             7200..8912
                     /locus_tag="PWS82_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906796.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8915..10684
                     /locus_tag="PWS82_001683"
     CDS             8915..10684
                     /locus_tag="PWS82_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906797.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10889..11455)
                     /locus_tag="PWS82_001684"
     CDS             complement(10889..11455)
                     /locus_tag="PWS82_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0906798.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11455..12411)
                     /locus_tag="PWS82_001685"
     CDS             complement(11455..12411)
                     /locus_tag="PWS82_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL0906799.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12411..13067)
                     /locus_tag="PWS82_001686"
     CDS             complement(12411..13067)
                     /locus_tag="PWS82_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0906800.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13437..15455
                     /locus_tag="PWS82_001687"
     CDS             13437..15455
                     /locus_tag="PWS82_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0906801.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15525..15731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15882..18296
                     /locus_tag="PWS82_001688"
     CDS             15882..18296
                     /locus_tag="PWS82_001688"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL0906802.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         5655 a         3449 c         4071 g         5313 t
ORIGIN      
        1 ttatgtaact tcaaatccag ttttgagtga acaaaattta ctcaataaaa gataacacca
       61 cagtgtggtg gcgatagcga gaaggataca cctgtaacca tgccgaacac agaagttaag
      121 cttcttagcg ccgattgtag tgaggggttg ccccttgtga gagtaggacg tcgccacgct
      181 taagttatcc atggaggttt agctcagctg ggagagcatc tgccttacaa gcagagggtc
      241 agcggttcga tcccgttaac ctccatatga gccgttagct cagttggtag agcatctgac
      301 ttttaatcag agggtcacag gttcgagccc tgtacggctc atatttttgc gggtgtggcg
      361 gaattggcag acgcactaga tttaggatct agcgccttac ggcgtggggg ttcaagtccc
      421 ttcacccgca taatttaagt tttacatgcc ggcttagctc agttggtaga gcatctgatt
      481 tgtaatcaga gggtcgaggg ttcaagtcct ttagccggca tctttaaaca cgcggaaata
      541 gctcagtggt agagcaccac cttgccaagg tgggggtcgc gggttcgaac cccgttttcc
      601 gctttgccaa agcaatcgcg ccggggtggc ggaactggca gacgcacagg acttaaaatc
      661 ctgcggtgag tgatcaccgt accggttcga ttccggtcct cggcatttca aatatgcgcc
      721 catagctcaa ctggatagag tgtttgacta cggatcaaaa ggttaggggt tcgactcctc
      781 ttgggcgcgt taattaaacg ggaagtagct cagcttggta gagcacttgg tttgggacca
      841 aggggtcgca ggttcgaatc ctgtcttccc gataattata aatttaaaca cggggcctta
      901 gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc
      961 tccatttcat ttaaataatg aatatctttt tcgcggtgta gctcagctgg ctagagcgtc
     1021 cggttcatac ccgggaggtc gggggttcga tcccctccgc cgcgattttt aagagcctgg
     1081 acctttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc
     1141 ctacaaggtc cattgtggcg ttgtaagttt catatggagg attacccaag tccggctgaa
     1201 gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc aagggttcga atcccttatc
     1261 ctcctttttc atattatcgc gggatggagc agtctggtag ctcgtcgggc tcataacccg
     1321 aaggtcgtag gttcaaatcc tgctcccgca attaaaaact aaaaacactg gttccgtggt
     1381 gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc ccgtcggaac
     1441 cgccatgcgg gtgtagttta gtggtaaaac cacagccttc caagctgttg tcgcgagttc
     1501 gattctcgtc acccgctttt agttttttat gggcctatag ctcagctggt tagagcgcac
     1561 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccattagta caaaatctta
     1621 ttggttttgg ggaagtactc aagtggctga agaggcgccc ctgctaaggg tgtaggtcgg
     1681 gaaaccggcg cgagggttca aatccctcct tctccgtttc aatattttgt tttacctggc
     1741 ccgttggtca agcggttaag acaccgccct ttcacggcgg taacacgggt tcgaatcccg
     1801 tacgggtcat taatttaata tagttttaca tggaggatta cccaagtccg gctgaaggga
     1861 acggtcttga aaaccgtcag gcgggtaaaa ccgtgcaagg gttcgaatcc cttatcctcc
     1921 tttttcatat tatcgcggga tggagcagtc tggtagctcg tcgggctcat aacccgaagg
     1981 tcgtaggttc aaatcctgct cccgcaatta aaaactaaaa acattggttc cgtggtgtag
     2041 gggttaacat gcctgcctgt cacgcaggag atcgcgggtt cgattcccgt cggaaccgtt
     2101 tggctcggta gctcagttgg tagagcaatg gattgaagct ccatgtgtcg gcagttcgat
     2161 tctgtcccgc gccattatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
     2221 tgtcgcgagt tcgattctcg tcacccgctt ttagttttta tgggcctata gctcagctgg
     2281 ttagagcgca cgcctgataa gcgtgaggtc gatggttcga gtccatttag gcccatttat
     2341 tttattggcc cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt
     2401 cgaatcccgt acgggtcatg gaagatactt tttgagtatc tttttctttt tatctgaaag
     2461 aatgatttta aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg
     2521 acagttctaa tgtaaaatat ttttatattt tttaagcaaa aaaatgaaaa aatacgagca
     2581 gaaaactgtt ttatctgcta tacttggttc gttgcaagtt cccgaaagga tccgcaggac
     2641 ggagatgcct tgtaaccgaa tttaactagg gggaatcatt ataatggtag aaagacactt
     2701 atttacatca gaatctgttt ctgaaggaca tccagataaa attgctgacc aaatcagtga
     2761 tgcaatcttg gatgcaattt taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc
     2821 tgtaacaact ggtcttgttt tagtatttgg agaaatatct acgacggcat atgtagatat
     2881 tcaaaaagtc gtacgggaaa caataaaaga aattggttat acacgtgcaa aattcggatt
     2941 tgatggagat acagcagctg tattggttgc gattgatgaa caatctcctg atattgctca
     3001 aggagttgat gaagcacttg agatccggga tgaagataaa aaagacgtat tagatgagat
     3061 tggtgctggt gaccaaggtt taatgtttgg gtttgctgta gatgaaacac cagaattgat
     3121 gcctttacca atcgctttga gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc
     3181 aaatgaatta acgtatttac gtccagatgc aaaatctcaa gtaacggttg aatatgatga
     3241 tcaaggacaa ccggaacgcg tagatacaat cgttatttca acacagcatg atgatgcagt
     3301 agataatgaa acaattcgtc atgatgtcat cgaaaaagta gtgaaggaag ttattccagc
     3361 tgaattatta gatgatcaaa cgaaatatta tatcaatccg actggccgat ttgtcattgg
     3421 tggtcctcaa ggggatgccg gattaacagg aagaaaaatc attgttgata cgtatggcgg
     3481 ttatgctcgt catggtggcg gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc
     3541 tgccagctat gctgcacgtt atattgctaa aaacattgtt gcagcagggc ttgctcgcaa
     3601 agcagaagta cagctagctt atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa
     3661 tactttcggt actggaacgg ttccggaaga agaactgatt gctgcagtga gagaaaactt
     3721 cgatcttcgg ccagcaggaa tcatcgagat gcttgatttg cgtcgtccga tttacaaaca
     3781 aacagctgct tacggtcatt ttggtcgtac agatgtagat ttgccttggg aacatacgga
     3841 caaagtagat gcgttgaaag caagtttagc aaaataagtt atattcagct ggaacgatcg
     3901 ccaagtggtg gttgttccag ctttttttat ggcagttaac ctcttgaaaa agatagtaat
     3961 tatttatcta tttttagatc ctgtcataat gaaagggagg aataagatga aaaaagaaac
     4021 aaatgttact ttggtcacag tgagtatttt cattgcaacg ttcatgacgg ctattgaagg
     4081 tacaatcgta acaaccgcta tgccaacgat tgtcggttca ttacatggaa tggaaatcat
     4141 gaactgggta ttttcgattt atttgctgac aaacgctatg cttactccga tttacggaaa
     4201 acttgcggat aaagtgggac ggaaaccaat ctttattttt gggacattat tgtttattat
     4261 cggttcgtta ttttgcggtt tatctcaaag catgattggg ttgattgctg ctcgtgcgat
     4321 tcaagggatg ggtgctgggg caatgatgcc agtagctttg acgatcatcg gcgatcttta
     4381 ttctattgaa aagcgagcaa aagttttagg tttgaatagt tcagcctggg ggattgccag
     4441 tgtttttgga ccgcttgcgg gaggattcat cgttgaaacg atcagctggc actggatctt
     4501 ttttatcaat gtgccgatcg gaattctatt aattattttg atttggctat atttaaatga
     4561 accaaaaatc atacatgagt ctaaaccaat ggatattttt ggtagtttta gtttgatggc
     4621 catgcttgtc tcattgctgt tagggttcca attattagga gacgaaggaa tgagtccgct
     4681 tgtttctgct ttgctgctag gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa
     4741 aagagctaaa gatcctgtca tcatgatgca tttattcaaa aatccactat ttgttgtggt
     4801 caacttggct gcagcattgg ctagtggttt tttgatggga atcgatgttt atatccctat
     4861 gtggatgcaa ggtgtattag ggctgaatgc agcaattggt gggctggtat tagcaccatt
     4921 atcactagtc tggatggtcg gttctttcct tgcaagcaag tggatgactc aaatgaaaac
     4981 tcattgggtc ttgagaattg gactactaat cgcagctatt gggggcgctt ggttgtatct
     5041 tatgcctttc agaagtaact atttatggtt ttttgctata tcaacggtca tcggtatagg
     5101 cttaggtgtg accatgacta cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca
     5161 gcttggtgta gcaacttctt ttaatacgtt agttcgaaca attggtcaaa cagtcatggt
     5221 ttctattttt ggtttattga taaatggggt aactaaacga gaacttgcag cagcaaattt
     5281 aacaaatgat gcagatatca tgaataaact ggtgaatcca caaacggcta aatatattga
     5341 tgcggatatt ttgttaccat taagaaaaat tctttatgat gggttacatt ccgtttatct
     5401 agttggcttg ttactcgtga tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag
     5461 tggtaaaata aaacagtagt ttcggttaag atatgtacgt agccgaatca ttcaaattta
     5521 aagttattca aaaataactt cttactaagg aaggcgaaaa tatggcattt attcaagcaa
     5581 atatttattc gaatgtgtta gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa
     5641 caaaaaaggt gattggcacc agttcagagc aagagacaac ggatatacct gttttatatt
     5701 tacttcatgg aatgggtggc aatcatagtg tatgggaaag acgtacgtcg attgaacgtt
     5761 atgtagcaaa ctacggatta gctgtcatca tgccatctac tgatcttggc tggtacacag
     5821 atacagttta tgacatgaag tattggacat tcattgcaga agagctacct gagatttgtc
     5881 atgaattatt cccgcaatta acaagaaaaa gagaaaaaac atatgccgct ggattatcga
     5941 tgggcggata tggcgcgtta aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa
     6001 tctctctttc aggcgctgtt tctgtaggaa atcgaatgga tgatcttttg atggttcgta
     6061 aagcatcttt ttgggaaggg atctttggac cattggatca aatcgaaggt tcaaaaaatg
     6121 atccgcaata cttattgaaa gaactagtaa aaagcggaaa accagttcca agattttata
     6181 tggcttgtgg ggaatctgat tttctttatc aggcaaatca ggaaatggta tctgctatgc
     6241 aggaacaagg cttggcggtc acctttgaac aaggacctgg cgatcataac tggatcttct
     6301 gggaccaatg gattcaacgt gcattggcgt ggctaatgga agaaaaataa aaaaagagtc
     6361 ttaaaaagat agctcctata gagatcacaa acatagtgat ctctatagga gcttttttac
     6421 tgctcgtttt ctcttctttt attttgttgg cataagcctt caatataggt ttgtgcactc
     6481 gttagaaaga gtgctttttc attcaattct gcatgggact gcatactttc tttaaatccc
     6541 tctaattgat tttcaactac ataattacgc ataaatagaa ctgtttcttt caaaaattca
     6601 tttccgtcca taattttttg tcgaagctgc tgttcatgct gctgatccat taactgtcca
     6661 atcaataaat tgtggagaga ggcaatggcc tgatgacttt gggtgactga aaatccttca
     6721 cgaacaagta attggatcat ttgctccaaa tgttctaatc gactcggaaa agcgggcaca
     6781 gtattgattt ctaattctgc cccacaaggg aaacgtgtat aaaggtcata gtaattttcc
     6841 atatactgca gcaactgttc attccaaggc aaagttggat ctggaagcgc taattcttct
     6901 tccattgttt cagccattat ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa
     6961 gcaggtgctt gaacatgaat tctgtttgcg atgtttcgca tggaaagctg cgaaagagct
     7021 tgtgtttcag ccaggatatc aaaggcggcc tgaatgatga ttgcttgaga aagctttatt
     7081 tccattttat caacctcgtg tattgcatta tctttttaaa catggtaaat tgttagtcgg
     7141 tctaatttta cagtgttaaa ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa
     7201 tgaaattaat cttacgacat gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt
     7261 ccgtagcagt catggttgca gcagcattat ggcaacctaa gttactacag caagtattgg
     7321 aagctattat tacagaagat aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc
     7381 tggctttgct aggattagcc gcaggtgtaa cgaacacgat tttctctgca aaagtagctc
     7441 aaggcgtaag tgcagatatc cgagaagaag catttcgaaa gatccagaca ttttcatttg
     7501 gaaatatcga acagttctct gctggaaatc ttgttgttcg tctaacaaat gatatcacgc
     7561 aaatccaaaa tttagtgatg atttctttgc aatcactttt tcgaattcca ttcttattta
     7621 ttggtgcctt cattttagct atgatcacga tgccgcaatt atggtggatc atcgtagcgc
     7681 ttatcgttac ggtatttctg attacggcac tttcatttac aaggatgggc aagcacttca
     7741 tgatcattca aaaactgatt gatcgtgtga atagcattgc aaaagaaaac ttactaggga
     7801 ttcgtgtagt gaaatctttt gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa
     7861 gtgaggactt aacaaaacac aacatcatag tcggtacatt attttctgtg atgatcccgt
     7921 ccttcatgct ggctgccaat ttggctgtgg ttggggcgat cttttttgtg agcgatcttg
     7981 ttaaagatga tcctacgtta attggtggaa ttgcttcatt catgaactac ttgatgcaga
     8041 ttatgatggc aatcattatt ggcggaatga tgatgatgat gacttcaaga gcagccgtat
     8101 cgctgaagcg gattgcggaa attttagatg ctgaaccaga tcttacttat ttagacgttc
     8161 cagaacaaga gttgacgggt tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg
     8221 aggatacaga tacgcttaaa gatatcagct tttctatcaa acctggagaa atggttggta
     8281 tcgtcggggc gactggtgcc gggaagtcaa cactagcaca attgatccct cgtctatttg
     8341 atccgacaga aggcaaagtt gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga
     8401 gtttaagaca aacggtttcc tttgttttgc aaaaagcaat tttattctca ggaacgattg
     8461 ctcaaaactt gcgccaaggg aaaaagaatg caacagaaaa agagatggaa catgcttctt
     8521 ctattgctca agcaaaagaa ttcatcgaaa agttatctga tcgttacgaa gcaccaatag
     8581 aagaacgtag cagtaacttt tctggtggtc aaaagcaacg tttatccatt actcgtggcg
     8641 ttatcggtaa tccaaagatc ttgatcctag atgacagtac gagtgcactg gatgctcgct
     8701 ctgaaaagct ggtccgagaa gcactcgatc gagatttgaa gggaacaacg acgattgtca
     8761 tcgcacaaaa aattgcttct gttgtcaaag cagaccgcat cttagtattg gatgaaggca
     8821 gattagtcgg cgaaggaacc catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa
     8881 tctttgaaac tcaaaaagga acggaggagt aagtatgact gatctaataa aagcaagtaa
     8941 atttttctat cactatttga agcggtacaa actctccttt ttctttatct ttatcacagt
     9001 gatcattgct acctatctac aagtaaaggc acctcaatac atcggggaag cttttcaaga
     9061 attagctaat tacgtaggag gcttgatgca aggcgtagac gataaaagca aatttgtttc
     9121 tgtaatatgg aaactcttgc tgttttatgt actagctagt gcagccaact ttatctatag
     9181 tattttgttc acacaagtag tagggaagtc aacgaaccga atgcgtatcg gtctattcaa
     9241 caaattggaa aaattgacga tccgtttctt tgattctcat caagacggtg aaattttaag
     9301 tcgttttacc agtgacttag ataatattca aaatagtttg aaccaagctt tgcttcaagt
     9361 catcacgaat gctgttttac ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga
     9421 gttagcttgg gctacaattg cttccactcc agtagccgtt ttgatagctg tcttgattat
     9481 ccgcaaagca cggaaatatg tggatcttca gcaagatgaa gtggggaaac taaatggtta
     9541 tatggatgaa aaaatcagcg gacaacgtgt catcatcaca aatggcttac aagaagaaac
     9601 gattgaagga ttcttagagc ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc
     9661 ttattctggt ctactatttc cgatgatgca aggaatgtca ctggtcaata cagcgatagt
     9721 tatctttttc ggcggctggt tagcattaaa tggtgatttg gaacgttcgg tggcattagg
     9781 cttagttgtg acattcgtcc agtattcaca acaatattac caaccactga tgcagatttc
     9841 atctggatac agcatgatcc aattagcgat tacaggggca agacgattga atgaaatatt
     9901 tgatgaaaaa gatgaagtca aaccagaaaa ctgtaaagca tttgatggtg ttgaaaaagg
     9961 tcttgcgttg cagcacgtag attttggtta ttctccagaa aaactagtct tgaaagatgt
    10021 ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt ggtccaacag gatcggggaa
    10081 aacgacgatc atgaatctgc taaaccgttt ttacgatgtc aatgatggtt cagtagtcat
    10141 cgatggaata gatatccgag attatgatct tgatgcatta cgtagacatg taggaatcgt
    10201 tttgcaagaa tctgtattgt tctccggaac gatcaaagac aatatcacat tcggcaaacc
    10261 ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa gcgaatattc atgacttcat
    10321 catgacgtta gaaaaaggtt atgatacaga gatatccgaa gaaaataact tattcagtac
    10381 aggtcaaaag cagttgatca gtattgctcg aacgatcatc acaaatccat ctttattgat
    10441 tttggatgaa gcaacaagta atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat
    10501 ggatgaagca atcaaaggaa gaaccagctt tgtgattgct caccggctga aaacaatctt
    10561 gaatgcagat agaatcgttg tcttgcgtga tggggaagtc attgaagaag gcagtcatca
    10621 cgagttatta gcagaaaatg gattttatgc ggaactttat cataatcaat ttgtatttga
    10681 ataaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca
    10741 gcttctcatg tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg
    10801 tgaataaaga ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa
    10861 catatcattt atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag
    10921 gcggctggtt cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca
    10981 gtttggaaca aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt
    11041 agacgacaag gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg
    11101 tgcgtgtagt ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat
    11161 tgaaaatccc tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc
    11221 cttgtagaaa caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag
    11281 catcgattgc ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt
    11341 ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg
    11401 gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac
    11461 gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc
    11521 atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc
    11581 ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg
    11641 gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata
    11701 gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat
    11761 atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg
    11821 taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc
    11881 atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt
    11941 tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc
    12001 aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg
    12061 taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt
    12121 aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc
    12181 gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga
    12241 accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca
    12301 gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc
    12361 gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc
    12421 ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg
    12481 cacagccaag aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc
    12541 agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat
    12601 gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga
    12661 atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc
    12721 gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca
    12781 attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc
    12841 cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata
    12901 aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga
    12961 tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg
    13021 acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt
    13081 tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa
    13141 ccaatgtatg attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg
    13201 aaagacattt tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa
    13261 atccttttaa acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat
    13321 tacggtaaat tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa
    13381 tattaagaga agttataatt attaataata gataaattga agttggagga tagaagatgg
    13441 aaaacattgc aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa
    13501 aacgaggggc agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag
    13561 tacagccggt tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata
    13621 cagcggcatt tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc
    13681 tatatgcatc agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt
    13741 tatctcaagc accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg
    13801 tctacatgga tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc
    13861 gacaatatcc ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt
    13921 catttggcaa cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag
    13981 atgcgacagc ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa
    14041 ataatatcat cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag
    14101 gcggtggagc gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa
    14161 gcaattctgg taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg
    14221 gtgattctgt ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat
    14281 ggaacaacat ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc
    14341 aggaaaatgg ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg
    14401 gtacatcaaa cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg
    14461 tttggaagat ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata
    14521 tcaagaacaa tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta
    14581 atgctggagc aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct
    14641 caagttcttc taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt
    14701 ggagcgtctc aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc
    14761 aaaataactt tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca
    14821 gcacgtcagg taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg
    14881 gcagcagttc gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg
    14941 caaataaaaa cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact
    15001 tcatttatcc aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag
    15061 gttcaactac ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat
    15121 catccactgg taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga
    15181 acaaacatca tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca
    15241 tctatccagg acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat
    15301 cagttactgg ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg
    15361 acagtcatgg aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta
    15421 tttatccagg tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat
    15481 tgaattccac aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag
    15541 gaagtagtaa aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg
    15601 cttttgaact cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac
    15661 ggccgttaca acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt
    15721 acgtcctata aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga
    15781 gaaaacaaag caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta
    15841 taatagaaac aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag
    15901 aaattgagaa aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg
    15961 atcctgaaaa gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc
    16021 tacatgtagg gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa
    16081 gtcaaggata caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac
    16141 aatatgcgct agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat
    16201 tccgacgcca aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa
    16261 ctgatccgga gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc
    16321 tagcttatga agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa
    16381 atgaagaagt gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa
    16441 tgcgccaatg gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat
    16501 tagttgattg gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag
    16561 gagcaaatat tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac
    16621 gaccagatac tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta
    16681 gagaaattac gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta
    16741 aaaaatcaga tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg
    16801 cttatgcaat caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat
    16861 tatcttcata cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg
    16921 aattcgctca aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa
    16981 aagctgctta cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca
    17041 aacaagaagc aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag
    17101 aagtcagcta tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa
    17161 tcccaatcat ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat
    17221 tacggttgcc taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa
    17281 atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa
    17341 cgaatacaat gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc
    17401 ataacaaaaa agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct
    17461 acattggtgg agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat
    17521 tcttatacga tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag
    17581 gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc
    17641 ctgatgatgt ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg
    17701 ggccattaga tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct
    17761 tggatcgtgt atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta
    17821 cattgaatga tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag
    17881 actatgaaaa tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag
    17941 cctataaagt ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac
    18001 cgattgctcc tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt
    18061 cttacgcacc atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg
    18121 ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag
    18181 atgagctaga aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa
    18241 cagtcagaaa agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg
    18301 ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc
    18361 attctgtctc agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg
    18421 attttcatgt tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc
    18481 tctgtaat
//
LOCUS       DANJJW010000054        18149 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000054 DANJJW010000000
VERSION     DANJJW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(29..727)
                     /locus_tag="PWS82_001689"
     CDS             complement(29..727)
                     /locus_tag="PWS82_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL0906803.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="PWS82_001690"
     CDS             complement(720..2072)
                     /locus_tag="PWS82_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL0906804.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="PWS82_001691"
     CDS             complement(2295..3695)
                     /locus_tag="PWS82_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL0906805.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="PWS82_001692"
     CDS             3856..4593
                     /locus_tag="PWS82_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL0906806.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="PWS82_001693"
     CDS             4718..5197
                     /locus_tag="PWS82_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL0906807.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="PWS82_001694"
     CDS             5194..5961
                     /locus_tag="PWS82_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906808.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="PWS82_001695"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="PWS82_001695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0906809.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="PWS82_001696"
     CDS             complement(6864..7466)
                     /locus_tag="PWS82_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0906810.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="PWS82_001697"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="PWS82_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="PWS82_001698"
     CDS             8147..8731
                     /locus_tag="PWS82_001698"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0906811.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="PWS82_001699"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="PWS82_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL0906812.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="PWS82_001700"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="PWS82_001700"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL0906813.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="PWS82_001701"
     CDS             10632..10811
                     /locus_tag="PWS82_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906814.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="PWS82_001702"
     CDS             10833..11858
                     /locus_tag="PWS82_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL0906815.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="PWS82_001703"
     CDS             12033..13277
                     /locus_tag="PWS82_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL0906816.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="PWS82_001704"
     CDS             13430..14377
                     /locus_tag="PWS82_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0906817.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="PWS82_001705"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="PWS82_001705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL0906818.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="PWS82_001706"
     CDS             complement(15279..15521)
                     /locus_tag="PWS82_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906819.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="PWS82_001707"
     tRNA            15642..15714
                     /locus_tag="PWS82_001707"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="PWS82_001708"
     CDS             complement(15833..16471)
                     /locus_tag="PWS82_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0906820.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="PWS82_001709"
     CDS             complement(16594..17226)
                     /locus_tag="PWS82_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0906821.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="PWS82_001710"
     CDS             17577..17876
                     /locus_tag="PWS82_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0906822.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6227 a         3163 c         3575 g         5184 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaataaa gaaacagatg tttctttata
    18001 ttgtatttta tcctttaatt aacacaaaaa cagctagtat aaactaataa tgttaattaa
    18061 aagtgtattt tatgaaacta tcgttattta ttaatgttgc atttttgtaa gttaaaaatt
    18121 ggaaatatag attgaaaaaa tgaaagcaa
//
LOCUS       DANJJW010000055        17447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000055 DANJJW010000000
VERSION     DANJJW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..966
                     /locus_tag="PWS82_001711"
     CDS             <1..966
                     /locus_tag="PWS82_001711"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906823.1"
                     /translation="VDDSVTSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQ
                     NELEVKQAKAENERAKKELLTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNE
                     KGFNQDYAFTSKDLSDSEIGSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVA
                     TSLHDQKVAILNEQYEKMQIYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTL
                     RTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLS
                     LSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYKLFNETKL"
     gene            1010..2182
                     /locus_tag="PWS82_001712"
     CDS             1010..2182
                     /locus_tag="PWS82_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0906824.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            2169..2873
                     /locus_tag="PWS82_001713"
     CDS             2169..2873
                     /locus_tag="PWS82_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0906825.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2878..4038
                     /locus_tag="PWS82_001714"
     CDS             2878..4038
                     /locus_tag="PWS82_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0906826.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            4114..4392
                     /locus_tag="PWS82_001715"
     CDS             4114..4392
                     /locus_tag="PWS82_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0906827.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4373..4744
                     /locus_tag="PWS82_001716"
     CDS             4373..4744
                     /locus_tag="PWS82_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL0906828.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4760..5164
                     /locus_tag="PWS82_001717"
     CDS             4760..5164
                     /locus_tag="PWS82_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906829.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            5161..5571
                     /locus_tag="PWS82_001718"
     CDS             5161..5571
                     /locus_tag="PWS82_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906830.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            5632..6219
                     /locus_tag="PWS82_001719"
     CDS             5632..6219
                     /locus_tag="PWS82_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0906831.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            6292..6759
                     /locus_tag="PWS82_001720"
     CDS             6292..6759
                     /locus_tag="PWS82_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDL0906832.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6805..7113
                     /locus_tag="PWS82_001721"
     CDS             6805..7113
                     /locus_tag="PWS82_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906833.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            7308..14042
                     /locus_tag="PWS82_001722"
     CDS             7308..14042
                     /locus_tag="PWS82_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0906834.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            14046..14918
                     /locus_tag="PWS82_001723"
     CDS             14046..14918
                     /locus_tag="PWS82_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL0906835.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14915..15964
                     /locus_tag="PWS82_001724"
     CDS             14915..15964
                     /locus_tag="PWS82_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906836.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            15968..16792
                     /locus_tag="PWS82_001725"
     CDS             15968..16792
                     /locus_tag="PWS82_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906837.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16810..>17447
                     /locus_tag="PWS82_001726"
     CDS             16810..>17447
                     /locus_tag="PWS82_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL0906838.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         6266 a         2651 c         3700 g         4830 t
ORIGIN      
        1 gtagatgata gcgttactag tttaagtagg ctagaattta taaaaatatg tgcatctgtg
       61 tttggtccta ttttgactgt aatggtattt gtgaatactt taaatatgca gaaaaaaaca
      121 gagcaaaagc aaaatgagct tgaagtaaag caagctaagg ctgagaacga gagagcaaaa
      181 aaagagctgt taacacagat agatcgtgag ttctattttt tattaaatat gtttattgaa
      241 atacaaaaag atcaaaatgc caaacaagct gttattgctt tacagaagtt ggcaattttt
      301 aacgaaaagg gttttaatca agactatgca tttacttcta aagatttatc agatagtgaa
      361 attgggagta caattgaaca atcaggaccg ctgaagggca cagttgaata tataaaattt
      421 gaaacatttg atgtaagcaa gtcgatgggg aattatcaaa caaaaacaaa agacgttgct
      481 acaagtttac acgatcagaa agtagctata ttgaatgaac aatatgaaaa gatgcaaata
      541 tatcttggac ggtacttcaa aatgtttcac cgaatagtta aaacattaaa tgagtattat
      601 gacgattata atgattttga tgtgaagagg tatacaaaat atatagggac attaagaaca
      661 caaatttctc cagctgaatt ccaagtgatt ttattcaatt ctctatatat aaaacgaggt
      721 tttggcttag gaattcaact tattggaagt ggtttttttg gagatgattt tgattttgaa
      781 actaatcaac attttgaaac aagtatcaat gaacagtggt ttctatcact atcaactgtt
      841 gacagtgata actctataaa gcgacaaaaa ttatctgaat atattaaaga attaggatcc
      901 gtagaatata aaaaaatagc taattttgaa agtttatata aactctttaa tgaaactaag
      961 ctctagaaat agggcttttt tatttttctt tgaaaggtgg tgaaaacatg tgagcttatt
     1021 tgatatcttt aagcagtcaa taagaaatga agagccttct gagtggatac cggattttgt
     1081 atatggagag gaagtatctg ctcgggcata cctcaaaatt atggcaaaaa atactgtttt
     1141 agattttgtt gcaagaacta tgtcaacgtt agaagttaag tttaaaaata aagatgggac
     1201 atcagactgg gattatattc taaatgttcg acctaattct gatatgtcag cagctacatt
     1261 ttggcaaaaa tttttttatc ggctcttaga tgacaacgaa gtgttagtaa tatttacaga
     1321 tgataatcaa ttgctgattg ctgatgattt tagtcgaacg gagtatgccg tgtatgatga
     1381 tgtttttaca aatgtgatgg taaaaaattt tgtctttgaa cgttcatgga acatgtctga
     1441 cgtcatttat atggaatata ataacgaaga attagaaagg tttacacgag gtttatttga
     1501 agattacgcc gcgttgttcg gacggattct agaagttgca atgcgtaaca accaaattcg
     1561 tgggtctgta tccattgatt caacgggaac ggcgaatgat gaaaaaggaa aagatggtaa
     1621 gactagatct caaagattac aagaatatat tgataggatt tggaactctt ttaaaactaa
     1681 ctcagttgca attgttccca aagtgaaagg ctttgagtat gaggaataca ccaataaaca
     1741 aggcgtatcg aatcaatcgc tagatgaact gaataagatg aagtcgtctt taattgatga
     1801 tgttgccaac gccataggag tacctacggc gcttatttat ggtgaaaaag ctgaactgga
     1861 ttcaaatatt aaagcttttc gtaaattgtg tattgctcca ctagttaaaa aacttcaaga
     1921 cgaattggtg gcaaaaataa ttagtcgtca agagtacaaa aacggggaac gtatagaagt
     1981 taaaaaagtt ctccaaaaag atctattaga gtatgctact caaattgata aaataacatc
     2041 tggtggtgcg ttttatattg acgaagttcg tgatgaagtt gattatgatg agttgccaaa
     2101 taatgaagga aaaattcgta ttcgtacgaa aaactatgaa gaaactgtga agggaggtga
     2161 gaacgacaat gccgaaagct aaaaaagtac catttcagtt tactaacgaa attcagaatg
     2221 gtaagcacat tctcaccttg agtggaaatg tacagaaaaa atattggcgt gatgatgatg
     2281 tcataaacgc taaagatatt cgtgactctc tagatactgt aaccgatgat attgtaatca
     2341 agttgaacag cccaggcggt gatgtgtttg aagggattga gatttacaat tatttaaaag
     2401 atcacccatc aaatattact gtagaagtta cagggcttgc cgcaagcgca gcaacgttta
     2461 ttgtcgctgg tgccaatgat gtgattatga atgttgggac ttctttgatg attcatgaag
     2521 cttctacatt tgcttggggg aataaacagg acatccaaaa aacgttaaac gcattggaaa
     2581 cgatcgatga ttctatctta gctatttatt cagataagac tggccaatct gttgataagt
     2641 tgcgtgaatg gatgaatgaa gaaaaatggt ttacagcaga tgaagctgtc aaatatggat
     2701 ttgccaattc tgtgaagcgt gaagaaccac aaaatgaacc tcaaaatatt gcagcgatga
     2761 ttcaagatgc tgttgccgtt gcaatagcga atttaaagga acctgttact aataaaatcg
     2821 aacaagaatc aaaacaaaaa tcattaatcg cacgattgcg aaaaggagaa taaagatatg
     2881 ttaaaaatta cagataaaac tgcagatgca aaaaaggcat ttaatgctat ttcttcgaaa
     2941 gaagatgcaa cacccgaaca aattaatgct gctctagaag aatatgtaac cgccattgca
     3001 gaagatgccg gcaaacaggt gcgtgctgaa tatgaagaat tgaaaaatgt gacggacaac
     3061 cgtgtattag aggctcgtgg aattcctaca ctaacagcag aagaaacaaa gttttataat
     3121 gaagccgtaa aatctggagg tttcgattct gacgtagttt ggccagaaac gattttggaa
     3181 cgtgtttttg aaaatcttca aaacgatcat cctattttag gcctcattaa ctttacacca
     3241 actgtaggtc gtgtcaaagt aattagagca cgtcgtaaag gtgttgctgt ttttggacca
     3301 ttgcataaag accttgaagg ccaactagat gctgaatttg gtgcaactga atttgttcaa
     3361 cttgcgttga ccgcattttt cttaatttct aatgatacct tagatttagg accacgatgg
     3421 attgaccgtt ttatcaatct ttctttaagt gaagcagtac gtgatgtttg ggctaaaaaa
     3481 attattacag gttctggtaa agatgagcca attggattgc ttaaagatct agatggtgcg
     3541 gtcacaagtg gtgtatatcc agataaagct agcgcaggga cattaacttt tgcgaaagag
     3601 aagattgttc ttgaacttgc aggggtgatg aaaaaattat ctaaatacac atacaaagta
     3661 gatggtgacg atgaaggtga agtgaaatac cgttctgttg ctggacgagt gtatctaatt
     3721 gttaatcctg ttgattactg ggatatcgta gctagagtta catttgcaaa tttaaacaat
     3781 gtgtatggga ctacgatgcc atttatcgct gttgatcata tcatcgaatc tgtagacgta
     3841 ccagaaagta aatgtattgc atacgttgat aaagagtatg aagctactca atcaagagct
     3901 gaaaaagtct atgtatataa agaaactttt gcaatgaaac gcgcaacttt gtatgctgtc
     3961 gacatgcttg gtaatggcta tccaacaaat aatgatgcag cacaagtata tgacttggac
     4021 tttacagcgg gggagtaatg tcccctgcaa ttggtgagat aacaccgacg actgatgggg
     4081 cagtcattaa tctttcgtaa atggggttag agtatgaaca ctgaagaatt tataaaagaa
     4141 tataaggccc gatttcgtat tttccatact tccgaagatg aggatatcgg gaaacagtta
     4201 gagagcgggt ttgccgatat aaaatctcta atcggcagtt ttgatccttc cacttatgag
     4261 aaaggaaaag aattagttta tgaacgtact cggtatctac ggaacgaatc actagagtac
     4321 ttttacgaca attttcaaac aatgattatg gatgcatcaa ttgatctggc aggtgctgga
     4381 tatgccaatt aaatcaaatt ataagaagcc taaaattgta gctggtgatc taaatacgcc
     4441 agttactttt tttgagtttc aacctaataa gggtccagaa cctggtgatg aagaaaaaaa
     4501 ggaactgtat ttttgtacag cactagttta caacccatca atgaaagatc gtgacattct
     4561 tggcacaaaa gaaggcgtta cgataaaaat acgggatccc catcaatctt atattcctac
     4621 taacaaacat aaagtaagga tattagatta tcgatatgga gataaagttt gggaaataat
     4681 cgatgtctca ccggatttgg aagaaaattc aattgtaaag atagtattag aattaattac
     4741 ttaaatttgg tgataaatga tgagtaacgt aaagtttgaa ggcgttcaag aaacaatcca
     4801 agctttagaa aaaaagtatg gagaaaagaa aactaagaca ttaacaaaaa aagcaatcaa
     4861 tgttggcgct gaaaaagtag aaaaacagtt gcaaacggac atgaccgttt ttaaagacaa
     4921 aggttatacg atcgctgagg ttgttcgcaa gaacgctacg tatagagatt acaaggcaga
     4981 agctgaaatc ggttggaatg gtccacatca acgatataga atcattcatt tgaatgaatg
     5041 gggatataca cgaaagggca aacaaattag accgagagga tttggtgtaa taacaaaatc
     5101 tcttaagaat tccgagctgt tgtactttga tgctgtagga gaggaggtta aaaagagttt
     5161 atgaaagata tgcttaatat catttacgag gctctttgtt tgaatgagta tattcattca
     5221 atgacctaca acgagaatac cgagcaatat cgtattaaat tttatgagca acctgaaacc
     5281 gccgataaaa caggtccaat gatcacaatt cgtcctgtag atacacctaa cgaagcgtac
     5341 cacggaagta acaaagagct ttctgtggag catatgatac agattgacgt tgaatcgaaa
     5401 tatagagcaa catgcaagca gattcaatat gagataaaaa aagagatgaa aaaactaggc
     5461 tttggccaaa tttccggtca agggttagat gaatacttta ctgaaacaaa ccgctttgta
     5521 gacgcgcgtc gttacgaagg aaatacaaaa atttacgata ccgaatatta gaaattaaga
     5581 cacgaaaata acgtgtcttt ttttattacc caaaattagg aggaaaattt tatgacttta
     5641 gtaggattca aaaaaatgac aatcggggtc tttgacagca cagggaagat tccagcagca
     5701 aatttatatg taattgaagg tgaacaaaac aaaggggcga ctgtatctgc tgaaatcagt
     5761 gggctatcaa aagaaccatc aaaagtttac ggatcgaaca tcgcttacta tgtctctcaa
     5821 aaaggaacag gcgatgtttc agctactttt ggattgttag atttaccaac agaagtaaat
     5881 gacaaaattc ttggttacaa aaccgataca aacaaaatca gcttcttggg agaagatact
     5941 gagccaccat attgtgcaat tttgatggaa tcggaggatt tgaacggcga tacagcaatg
     6001 cttgcgatgt tcaaaggaaa attcagtcgt gaatctatta atcttaacac tacgacaaat
     6061 gaagctttcg aaccagaagc agaagaatat gtattttcag cgattgccaa tgatgttgaa
     6121 ggtgatgcta aaggccaaac agtcgcaaaa tatgttggag acgaagaagc ttctattact
     6181 ggtttgaaag agatggtatt tcctgcagcg ggggagtaac tagccccgtt gttggagcaa
     6241 tcactcccac tattaatggg gcaacagttg aattaagtta ggaggataac tatgccagat
     6301 acatttaaaa tctataaaaa aaatggtact gaatttacaa aagtcgcaga gggtgaaagt
     6361 ccactttcta ttactggaat tgcagcaaat actcaagttg ctaaaggaga ttatcaagct
     6421 acgcgtgtgg tcggaggtca agaatcagta aaagtagata ttcctgcatt caccactctt
     6481 ccaatttcag ttactggtgt gacgttggat aaaacgacag ctgaagttga acaaggcgga
     6541 accttaaaat taactcctac tgtaacacca gcaaatgcaa cagacaaaac aggaagctgg
     6601 agcagttcga atacagctgt agctacggtt actggaggca acgttacagt aaaaacagac
     6661 gctgaagttg gcggaactac agaagtttca tttactacta cggatggcgg taaagtagca
     6721 aaatgtacta ttacagttat tgaaaaagca gcgggctagt cttcggacta gcttttttat
     6781 ttggaaaata atggaggaat ctaaatggca aaagtaagaa ttgaattgaa agaagaatca
     6841 ggaaagaaag tctatgaaaa catagacaca acagggaaag attatcgaaa agctttagaa
     6901 acaatcaaaa agctaaatga agacggagta atgatttgga atcgtttaga catttatttg
     6961 gattttgctg taggtatctt cagggatagc aaattgacag ctgaacaaat tcttgatggc
     7021 ttaccatctg agaaggtaat gagcacattg gacgacatcc taggagaagt aatggggatt
     7081 gaaagtaatc cagacccaga cgcaaaaaag taacacccga agaagcagaa gaaatgtact
     7141 taaacctatg cagagagtta gtgaaagcgg gatggtcact atctgacata gaagataatt
     7201 cctttgaaat gatgatgaaa attgcgtgca cttcgcctaa gaaagagaaa caaaaagaag
     7261 ttgatcttaa agacttcttg aaatccatgt agagaggagg taaacatatg gcaaatggaa
     7321 aacctttagg caatatgatt atcaaactag acttagacag ttcagctttt tcaaaaggtc
     7381 tagccggagc taaaaacgct gttaaccatc aaatgaaagc aatgaaatca cagatgcaag
     7441 taatgaactc ttcaggaaat tctctcggtg ctttacaagc aaaatacaat ggtttaggcg
     7501 gagttctaca agcaaatgaa aagcaagtag aattgctcac taaggcttat aaggatagtt
     7561 ttgactctaa cggaaacgct actgcttcga ctgctaaata cgctaatcaa ttgaatcaag
     7621 caacagctag atcagcaagt tacgaatctc agatgaaaac aactgttgga cagattgctc
     7681 ggatgaaagt ggaaacggaa gggtttacgg ggaaactaaa ggctaattct gatgtattga
     7741 ttaattctgg gaaaaaaatt gaagcctttg gtaaaggagt ttctgggata ggatctgccc
     7801 taactgttgg agtaacagct ccaatagtgg caggcgcaac tgctgttact agtgcagcta
     7861 tttcatggga aagtgctttt gcaggtgtaa aaaagacaaa tgatgaagtt gtagactcga
     7921 atggaaacgt agtttattcc tatagcgact tagagaaagg tctgcgtaat cttgccaccc
     7981 aattacctgg tagccatcaa gaaatagcaa atgtagccga agcagccggg caattaggta
     8041 taaagactga aaacgtagtt agcttcacta aaacgatgat agacatgggt gaatcgacta
     8101 atatgtcggc tgaaactgcg gcaacttctt tagcacgatt tgctaatatt acccaaatgt
     8161 ctcaaaaaga ctttgataaa ttaggatcag tcattgttga tctaggtaac aattttgcta
     8221 caaccgaatc agaaatcact gaaatgggat tgcggttagc gggagctggt aaacagattg
     8281 gaatgagcca aggcgaaatt ctaggatttg ctactgctct atcttctgtt ggtgtcgaag
     8341 ctgaagcggg tgggtctgcc ttttcgaagg tcatgatcca gatgcaacta gctgtagaaa
     8401 aaggatttgg cgcttttgat cagctgaaac agatggcaga agaacaaggt gttccgtggg
     8461 ttaatttagt aaatgctgtg agagacggtg gaaaatcgtt aaaagcagta tcagagcaaa
     8521 tgggattcac ttcatctgat ttaaagaaaa tgtataaaga agcggacaat tcgaaaagtt
     8581 cgcttgaaaa ttttgcgaat gtggcaggtg ttacaagtga acaattccag aagttgttca
     8641 agagtgatcc ttcagaagca atcatcaagt tcattcaagg gctgaaagat tcagaaaaac
     8701 atggtacttc agcaatcaaa gttctggatg acatggatat aaaagaagtt cgactacgcg
     8761 acagtttatt gagagctgca aatgctagtg acatattcaa aggagcaatt gaaaaaggaa
     8821 acggcgcgtg gaaacaaaat acagctttaa caaatgaagc taataaaaga tacgaaacaa
     8881 ctgaatcaaa gctaaagata ttgaaaaacg aagtgattga tacagcaatc gatcttggag
     8941 gtccatttgt agacgcgttg cgtgatggtc taaatgtatc gaaaccattg ataaaaaact
     9001 tagggaatat ggcgaaagct ttctcagcta tgagtgatga tcaacaaaga aacattatca
     9061 aatgggttgg attagcagca gcagctggtc cagcgctgaa gcttttagga actggtactt
     9121 cagtaatagg taaagttact tcaactactg gaagattgac gaaaagcctt gttgatttag
     9181 cagcgaaagc agcagaaaag aaggctatgg atgcctttgc atcgtcggta acaacagctg
     9241 gtgctgcagc tgcaaagaca gcaggagctg gaggtattgg cgggttagtc cctatgatgg
     9301 gaaaaacagc tagtgctgca agtactgctg cgggcgccag cggaataggc gcaatgacaa
     9361 cgtcactagg tttgttaggt cctgctctac ttggtattgt tggcgttggt ggcgcactgg
     9421 cagttggcta tggagcatgg aaattatttg gtgaagaagc ttggaattcc tcgcaaagag
     9481 taaagcaatg gggaacagat gtcggtgagg agatagatag tactcttgac ggagtacaga
     9541 agaatattga aggtgcaaat ggtcaattcg gtcttctcaa agagggtttt actgttgatg
     9601 atgcaaataa tatggcggct aatttcgcaa ccgctggaac atctttggaa acatctttaa
     9661 cgggtagaat aaaggcatta gacgatgcac taaaagggct acctcaatca gttcaggatg
     9721 ccatgaaaga agtagctgaa tcagaagaag aaaccttagg gcaatctctt tctaaaataa
     9781 aagacaacaa tgaagaaata aaaaaaatca gagaaagagc cgcaaatcag aacagagact
     9841 tgactgtttc agaactacaa cagatacagg atctaatgaa aggaacttct caagcgtatg
     9901 tggatacgct cgatgtttct gctaaagaca gaaaaaagat actatcagcc atgaatggtg
     9961 atgttgcaca agctacagaa gaagaagcga agacgtggtt acaatcttta gcgaaacaaa
    10021 aagctgcaac taccgaaaat tatagacaaa tgcttgaaga aaaaaaggcg gctttaaagg
    10081 aaaagggagt aagcaaagaa ttcattgatg cattagaaga tgaaatgaat gattacatca
    10141 aaactacaaa tcaaggattc gatagccaaa tagctgctat aacaaataaa tatccacaat
    10201 tgattaacga agtgagttta tcaaatggtc aattatttag caaaacggca gctgctatgg
    10261 acaatactgg tcagtataca aaagtttttc tcgaaagtaa tgaaaagatt ttagcccaag
    10321 cgaagaaaac atcggatgaa atagcagata atgccgaaaa aaatgcagaa caacttggta
    10381 tggtagctga tgaggctacg catgctggat caacttggaa tggcttagtt ttagacgaaa
    10441 aaacaggaaa agtaaaaact aatgtacaag aagtagtgaa tgaagcaacg aaggatttca
    10501 ctaaatggaa tgaattgaaa cctgttgttc atgatgcgaa actaacaacg aatgcaaaag
    10561 atgtcatttc catagcggca gtacaaaatg gctattggga tgatatggaa tggaaagaaa
    10621 aagaagcatt attagaagat gagtgttcta aaaatgtata caaggcttta gaaaatgctg
    10681 gtaaatggaa tgagttagat attccttcta aagaagcaat cctaacttca aatactcctg
    10741 aagttatggg tgaaactctc atgaatttgg gattatggga tacttacaaa cttcaagtga
    10801 aagatttaga tttgaatgcg tataaagtat acgatgctat cgatggatca gaagaaaaat
    10861 taaatagttg ggctcaatta gataacccca caaaagaatt gttattagat aactcagatt
    10921 tttctacaaa actttttaat tccgaagaaa tgtttaaacg ttggaatcag ttatcatcta
    10981 atgcaaagtt tctttcggct aataatacta aatttacaga ggttctttta tcatctgaca
    11041 gaatgtggaa tcaatgggta aaccttcctg ataatgagaa aagattactt gcagataacg
    11101 aagatttagc taccactgtt ttatcttcag aagaaatata cggaagatgg actcaattac
    11161 cagataatca aaaatatatg ttagctgaca atactgactt agcaaacaaa atattcgctt
    11221 cagaagaatc atggaatgca tggaaagcaa ttccagacac agttaaaaac ttgcttggta
    11281 ataatattga tttactggct aaagtgaaag acgggactgt aagtgtagaa gaatataaca
    11341 aagttatgcc acttcttaaa actctatatg gagataacgt taatttaaaa gaggcaatca
    11401 gtggggctaa aagctctata gagaattaca acaaaaatat ttttcctagt gataaaactg
    11461 cagtaggaca cgatgaggct tcacaagctg ctaaagatgc atttgaagcg ttcaacatct
    11521 tccaaacaaa aataccagat aaaattacta aaactgtatc agctgacttt ataggtccta
    11581 tgccgaaaaa tgcgaaagga acaaacttcc atcctggcgg agcagctatg gtaaacgatc
    11641 aaaaagggcc tacttatgaa gagcttataa ccttgccaag cggagaaact tttataccta
    11701 aaggacgtaa tgtcgtttta gacttaccaa gaggttcgaa agtactgaac gccactaaaa
    11761 ctaaacgtct agtgcctaaa tatgctgatg gtataggaaa cataacgacg gtttcatcta
    11821 cgctaaactt agatgcttta attttagcta ttaacgagct aacgacggta ttgagaactc
    11881 aactgatggc aggaacgact gatgctaaag atactaaact atcagatgaa acaaaagttg
    11941 ccgatccgat tattccggac agtctttctg aaaaatcaga tcagtatcta ggaattggag
    12001 ctcaatggtt aactaatcta atgaatggtt ggaactcagc ggttcctcaa tatatgaata
    12061 gtgaaatggt ttttattaca aactatctca atgcgctaaa acttcaaaac aatcctaatt
    12121 atacgcaagg agcaacatgg aataagaact tactgaatgg atggaatagt ttaactggga
    12181 cctttattgc tacaatcaat tcattttgta atcaagcaat ggtcacgctt agaaactaca
    12241 acactcctat gtacaacaat ggccgaacgt ggcagcaaaa taatcttaat ggttggaatt
    12301 cattgtatgg atcattcata gcccgtgtaa atcagcttgg taacgattcg attaacaacc
    12361 ttcgttctaa aagtggtggt ttttacaatg cagggacatt cttgttgcaa tctttaataa
    12421 acggaatgaa ttcgttaggt aattctcttt ctactacaat gaacaatgtc gctaatacaa
    12481 tggttggtgg tataggcaaa ggagttaatg gcgtcatatc gggagttaac tatgtgctta
    12541 aggaagtcga atcaagtaag aatatcggaa actggccaat tccacaatac gctaagggaa
    12601 ctgatggaca tcctggtgga cttgctatga ttaatgatca aaaaggccta gttcacgaag
    12661 aatatgttca gatgccagat ggacgtagtt ttattgccaa aggtagagat cttcttgtta
    12721 atttgcctaa aggtgctcag gtcttaaatg cttctttaac aaagaaatta aaggaacgtt
    12781 taaatattcc acactacgaa aaaggtattg gcaatttaga tattgttgat ttgttagatg
    12841 atgaaaaaag aatgttagaa ttcttaacta gcaaagttga tttttcaggc attaatgaac
    12901 catggcttga tatgactaaa tcaggaacgt cattaatgtc caaagctgca aatacaatgc
    12961 ttcaatcaaa attaagtgaa ttcttcactc atggcaattt tgatggtgca gtaaatgcca
    13021 atggtgttta tcaatattta gttgatgtgg cacagaaagt aatgggtaag ttccccggac
    13081 ttacagtaac ttctggatat cgagcaggag atgcctacta tcacggaaaa cggcaagcta
    13141 tcgacttggc ttatccaggt atttctggag atccgagata tacagcagca gcaaattacg
    13201 cttttgaaaa attcccttca aaaattgcgt atgtcattac gaatggacgt gtacgtgacc
    13261 gcatgggatt atctggaaca aggtcaagcg gacaatggac gaattggcca gatggcgatc
    13321 acttcgatca tattcattta aacggttcga tggggtctgg cgatatattc aatggaggag
    13381 ttggaggaag tggtgttgca cgctggcgtt cttatgtgtc taaagcattg aaaatgaatg
    13441 gattaccagc tacagcagcg tatataaatg cctggatgtc ccaaatccaa acggaatctg
    13501 gtggtaatga gaaagcaatc ggcggcaatg atgggctcgc cgaaggcaat gcaacaggat
    13561 tacttcaaac gaaacccgga acttttgcag ctaacgcttt tcctggtcac ggaaatatta
    13621 tgaacggatt tgataacatg ttagcagcaa tcaattatgc taagaaacgt tacggagttg
    13681 caggtatgtt gcaagttatc gggaaaggac atggatatgc caatggcggc ttgattacca
    13741 aagacggact atatcgagca ggagaaggaa ataaaccaga aatggttatt ccattgacaa
    13801 gaaaaacaag agctatcgaa cttatgggtc aagcattagc cttcctttct ggaagcaata
    13861 aaaaagaaac gaaaagtgag attaattctg tagataatag acatatccta caagcgatag
    13921 aaaaacagtc tcgtatgaca aatgatctgc tatcattatt actctcgttt tttaaaagta
    13981 acaatcgatc agataaagaa ctagctttag atattcagaa aattttaatc aggaggacat
    14041 agtatttgag gacagtaata ttaagaaata aattgaatga agaaattgat ttgtccacag
    14101 aagattattt tgctactgaa atgtcgaatc taggttttga agttacaaaa gagcatattg
    14161 gacaatgggg gagtttcaga gaaataggag agagtattga attgtctgaa tttcaatcat
    14221 cagttatgat ttctgttcac ggatttagag aacaacaatt atatggttca ttagttgaat
    14281 ttctggctaa tggacctttt gaactagaat tttcttttga ttcagaaaca atgatcagga
    14341 aatgtagttt gaaatcatta tctaaaacag aaattgattc ccaaagctca ttgctaacag
    14401 atactttgaa tctatatttt acttcaagct ggtattctat taagcgtgaa aaattaatac
    14461 agaattcaac ttctctcaat acacgtggaa aagtttactc atttgttcga ccatatgtct
    14521 acaccaaaaa tgtatgggaa aagaaaggtg tatttaagtt cagtaataat tctgtatatt
    14581 tgacaaatag tacagagcga atgtctccgt taaaaataag gattattgga gagtgctcaa
    14641 atccatattg ggaagttatc caaaactcta aagtagtagc ttcagatggc tattttatta
    14701 atatgaaaga cacacaaata ttagaagtgt ctagtttgct agaagaccaa acggcaattt
    14761 taaaagatgt aggtggagtt gaatcttctg tttatcaaca acaagatttt actaaatcaa
    14821 acttcgtcca agcgcctcta ggagaattca gcgttgtttt tcatgttgga catgcaaatg
    14881 tagagattga actttatgag gagcgtgatc tattttgatt ttagccatta ccctctttca
    14941 ccgagatatg aatttatata acgaacgttt aatttcgact gagttcgaat ttggaacgga
    15001 tgaaattgga gaagaagtaa gtaattttat tacagataaa tatgtccatg taaaatcagg
    15061 agattttcta ctagcaaaat ttattcctag cgggaagatt gcttattttg gagtgattag
    15121 ctcgcaagaa gacaaaaaaa taaattgcaa aggattactc agtttggccg atagcgagat
    15181 accgactgta aaaatatcag gtgctaatta tgaagagcat attcgacgac tgattgaata
    15241 ctacttattg aacgatccaa caaaacaact aaaaaatatt ttggaggtaa aaacagaaag
    15301 cgttacttct cattcttatc aagctgttga cacaaataag agacgattga atgcatatat
    15361 aataaatggg ttcaaaaaat ataatgttaa atggtatgtc aaagagatta aaggtaaaaa
    15421 aatatatact ggtattcgag cagttgatga ttcaatttat attaaagata attcatctga
    15481 gtttaatgac tgggatgttt ttgtgcaatc tcctggacca gggaacgaga acaaattatt
    15541 gatcgtagat aaagcaatga aagatataga aaatcccttg gtattgtcta cgtggtattt
    15601 agatgacgaa aataatctta cgcaagattc aacgaataag aacattgcat tgcctaccgt
    15661 taatttagta aatatatatg atcaaactca agaagataga ccttcttatg aagaggtagc
    15721 taaatcggag cttaaaggca acgcctattc acatgaaatt aaagttaatg ttgtgctaaa
    15781 tgctagaaat atcaatgtag aaaatatcga aacaggaatg ttagcgacta ttgtttacaa
    15841 agagcagact tacaaatctg ttctaactgc ctggcgaata tccagcagta aaaagaatat
    15901 agaattgaca tttggtaatg tccgaagtcg ttttatggat tattttgaag ataatggggg
    15961 ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag taagcgcaga
    16021 aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta ttgatggtta
    16081 cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg caggagcagc
    16141 gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag ttccagcaaa
    16201 ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac ctggatctat
    16261 tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag aatttataac
    16321 aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac cactatgctc
    16381 agttaattct actggatcaa ctgtttcaat ctcaaagata tcggatagtt acgagctgac
    16441 tctctctaaa ggagaaattt tgtggagggg gactgcgtta atgcatgata ctcaaactgt
    16501 ccaaccttca aaaaaaattt ggcagacagt ttcaggtttt ttgttagtat ggctcccata
    16561 cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata aggagcgtat
    16621 aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc acaaaaagtg
    16681 gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg cagcaagcaa
    16741 tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt agttaggagg
    16801 tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca gccttatcta
    16861 cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa tgtacaactt
    16921 cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt gaaatttgtg
    16981 ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga ttcgactaat
    17041 gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca attcaaacaa
    17101 gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa cttcatcata
    17161 gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat ttcttcattg
    17221 gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga taaacaggtc
    17281 caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa tgaatcagat
    17341 aagcaagtaa atgatcgtat tgataaggca aataatcgta ttgatcaaac caatcagcaa
    17401 atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcattga
//
LOCUS       DANJJW010000056        16492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000056 DANJJW010000000
VERSION     DANJJW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(4..1926)
                     /locus_tag="PWS82_001727"
     CDS             complement(4..1926)
                     /locus_tag="PWS82_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL0906839.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(2138..3034)
                     /locus_tag="PWS82_001728"
     CDS             complement(2138..3034)
                     /locus_tag="PWS82_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0906840.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(3027..3707)
                     /locus_tag="PWS82_001729"
     CDS             complement(3027..3707)
                     /locus_tag="PWS82_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906841.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3708..4490)
                     /locus_tag="PWS82_001730"
     CDS             complement(3708..4490)
                     /locus_tag="PWS82_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906842.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4483..5400)
                     /locus_tag="PWS82_001731"
     CDS             complement(4483..5400)
                     /locus_tag="PWS82_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0906843.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5564..5887)
                     /locus_tag="PWS82_001732"
     CDS             complement(5564..5887)
                     /locus_tag="PWS82_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906844.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            6675..9902
                     /gene="fss3"
                     /locus_tag="PWS82_001733"
     CDS             6675..9902
                     /gene="fss3"
                     /locus_tag="PWS82_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL0906845.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            10015..10329
                     /locus_tag="PWS82_001734"
     CDS             10015..10329
                     /locus_tag="PWS82_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0906846.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            10342..10716
                     /locus_tag="PWS82_001735"
     CDS             10342..10716
                     /locus_tag="PWS82_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0906847.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            10726..11061
                     /locus_tag="PWS82_001736"
     CDS             10726..11061
                     /locus_tag="PWS82_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL0906848.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            11144..12484
                     /locus_tag="PWS82_001737"
     CDS             11144..12484
                     /locus_tag="PWS82_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0906849.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            12562..13236
                     /locus_tag="PWS82_001738"
     CDS             12562..13236
                     /locus_tag="PWS82_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL0906850.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            13229..13393
                     /locus_tag="PWS82_001739"
     CDS             13229..13393
                     /locus_tag="PWS82_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906851.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            13469..14611
                     /gene="dcm"
                     /locus_tag="PWS82_001740"
                     /pseudo
     CDS             13469..14611
                     /gene="dcm"
                     /locus_tag="PWS82_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            14601..14891
                     /locus_tag="PWS82_001741"
     CDS             14601..14891
                     /locus_tag="PWS82_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906852.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            15150..16334
                     /locus_tag="PWS82_001742"
     CDS             15150..16334
                     /locus_tag="PWS82_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0906853.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            16331..16468
                     /locus_tag="PWS82_001743"
     CDS             16331..16468
                     /locus_tag="PWS82_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0906854.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         5237 a         2996 c         3298 g         4961 t
ORIGIN      
        1 catttataag ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa
       61 atttgctaat aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa
      121 ttcaggtttt actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc
      181 ccaatcaatt ccttctggat taacgtagac acagattact ggctttttta taaaatctac
      241 ttcgatatgc ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat
      301 tgctccctct agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat
      361 atttaaaacg ttcacttgat tatcctttat ttggtgggta aaaaagcaaa atcgtttatc
      421 taataaattt agaaaagcat cttctgtttg ttcatttaac tgtagatact gttttacttt
      481 tctgacatct gcccctaaca tcaaaggact aacaacaata acaggaaaat ttgtattttc
      541 aatcggaatc gtgcttataa caagttcatc agtgactttt ctatcttgta gttcattgat
      601 gcttagtaca tcttctatct caaccatatc tgcaaaaccg ttatttattc tttgctctaa
      661 taattttgat gttccatatc cgctactaca aactaatatc actttcgttt tgtcaggttt
      721 gtaacgatcc aaaagtgatt gaatatggag tgttacgtaa gcgatttcat cttcattaaa
      781 atgtatacag aatctctctt cgatttcctc taataaatct acacttatca taaatgacct
      841 tttaaaagaa tatttgattt tgtccgtata agggttatag atatttactc ccaatttgag
      901 tcttttaact gctgcattga gatgtaatac tagattattg atcaattctt catcaggata
      961 aaattcattt aatttaattc ttactaactc ttgtagctca acacccatat ctaaatagga
     1021 aagcttcgtt tgatctaagt cgtgattcat agcatttttt tcaatcgaag taatgtgaat
     1081 gtcaacataa gcttgttcat ctattggcac aataattgaa aatgcttttt caatcatttt
     1141 gattaaaata agtgtattct ttgagatttc ttcttcagaa ttattactac gtgataagaa
     1201 attgcttgtt tcaatccttt ctatcgcaat agcaaggtgg atagctaaag tttgaaattc
     1261 ataatctgta aatttcaatt gtgtcttatt catgtatcga ttaagcaatt cggctacttt
     1321 tttcaatatg tccttattta agaaattatt cgcttcacca atgaaattga tattcaagtc
     1381 taagagatct gttttactgt tagatgctgt aaagccaccc caataatagt tcacaacttc
     1441 tgacatcaac cttcttttac ttttttcatc agctttgatg cgcacgccat ttcgatctga
     1501 aactaaggac atcccatgct tgtttagtct ttttctaaca tctttcattg tgctttctac
     1561 cgtcgacctg ctcacgtata attcgtcagc aagattttga atctttagag aatcattagt
     1621 attcaataat ttagcaaaca cataagttag acggtcctcc ggagagtctg cgatattgat
     1681 tttatgactg gataataaat ttcgaagtgt ttcttttttt ccaataagcg aaacgccttt
     1741 tttaggttga accaacattt gtacatctgg cattttttgg attatttctt tcaaaattaa
     1801 aatatctttg ctaattgttt tcgtagatac atataattgt tctgataatt ctttgtaagt
     1861 tgtgtgctca ttttcaatca aaaagtatag tattttcatt tgccgttcgc taaattcgat
     1921 gcccattatc ttcacctcct gaatacagct taagtgtaca attattatct ctattttcat
     1981 acagaagaaa gttcttgatt aatgtagaag tttctaatat tcttgattat ttcaaatcaa
     2041 taaaaaagcc tgcaatattt atatcacagg cttttttctc tattaaatat atataagata
     2101 atacattttt ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg
     2161 agatggagaa gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca
     2221 atttttttga caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt
     2281 cggtataaaa acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat
     2341 tggccactgt taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat
     2401 ctgcataacg caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat
     2461 ctgctaatac agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag
     2521 aaaattgttc ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat
     2581 ggagatccat ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca
     2641 tactgagtaa tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg
     2701 tatcctctac ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga
     2761 gatcatgaga aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt
     2821 gtcctttttg ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat
     2881 tcttttctcg aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg
     2941 cgattttctt agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa
     3001 agataacaac gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta
     3061 tgaatataca cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc
     3121 actgttttct cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc
     3181 gaaaatactt ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc
     3241 aaatggacag ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc
     3301 ttgtaataga tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg
     3361 atgccgattc gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct
     3421 cctgctgcca acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc
     3481 ggaacattcg atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc
     3541 atgagatcta gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta
     3601 cctgaataag catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga
     3661 acagcttcat cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca
     3721 gatttttgga aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc
     3781 agaatcattt tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc
     3841 cacgtttccg taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg
     3901 ggaatgaagg ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt
     3961 ctagtgatga atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat
     4021 acaggaaata cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat
     4081 ccgaaagcaa gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt
     4141 gtgatcaaaa gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa
     4201 gataacgtat tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat
     4261 gctgcgaaag ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca
     4321 ccgttgatga ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac
     4381 cattcgttcc aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt
     4441 ttacggctct tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc
     4501 tccagttaat tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac
     4561 aggaactttt gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa
     4621 aagctggata acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac
     4681 cgttactgct tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc
     4741 caattcttct ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt
     4801 ggcgatctgt gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc
     4861 ttttaccaaa cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt
     4921 cgtcggttca tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa
     4981 acgctggcgc atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg
     5041 catcattttc agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat
     5101 acgtaggtta tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc
     5161 catacgtcgt cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc
     5221 aatcgagatc aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc
     5281 aatcaatcca tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc
     5341 agcaccgtat tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat
     5401 acttatcctt ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc
     5461 agaaatgtat aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa
     5521 tacgtgtctt tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt
     5581 tcttttcgac caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca
     5641 gatcaagctt gagtaatttt accagaccgg ttataaggat aactatagca agactccctc
     5701 ccagaaaaat tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac
     5761 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa
     5821 gtcgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
     5881 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
     5941 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
     6001 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
     6061 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
     6121 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
     6181 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
     6241 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
     6301 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
     6361 actgtgaagc tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt
     6421 ttgaaaaacc ttgatttata aaggttttcc cgtaaatata ttttattata tttgattaaa
     6481 tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta
     6541 gattaaattt ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta
     6601 aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga
     6661 aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga
     6721 gtttggctgt tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa
     6781 ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag
     6841 gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca
     6901 acggctttaa acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca
     6961 ctcctggcta tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg
     7021 ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct
     7081 ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa
     7141 atatcgcagc cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa
     7201 ccttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga
     7261 ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc
     7321 gtgtgaatgg taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc
     7381 tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac
     7441 agactttaat ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg
     7501 aaacggaggg ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag
     7561 gaacggtttt ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca
     7621 aagaaggcat tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa
     7681 aatcagtacc ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag
     7741 gcgaaaccat ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata
     7801 aaagcggagt tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata
     7861 cgtttgccat tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg
     7921 aaaacggtca tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt
     7981 atgtgaccga aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg
     8041 aattgaagta tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc
     8101 aagaagtcac tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac
     8161 aaggtaaggc agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag
     8221 ctgttaaatg gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg
     8281 aaacggtcac tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca
     8341 acgagtatta ttggcaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt
     8401 atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg
     8461 ttacggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag
     8521 ctgcgggcac taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag
     8581 ggaccaatga aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag
     8641 ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag
     8701 tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg
     8761 aaaacaaaga agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa
     8821 aacaaccaat taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg
     8881 ttagtttgaa ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc
     8941 ttgcgacttg gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg
     9001 ataaattgag ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca
     9061 ttgatgtgga tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga
     9121 ttgcagaaac aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc
     9181 ataaattaac cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg
     9241 aaaataagga agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata
     9301 accaagccca aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc
     9361 acctagaaaa cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg
     9421 tgtcgattac tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg
     9481 ctttattacc ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag
     9541 taaatgataa agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc
     9601 cagaaggaac caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg
     9661 gaaaacacaa tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa
     9721 ccatactaag tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat
     9781 ctagtcccac agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt
     9841 tcattggttt tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact
     9901 aaggtgatga gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa
     9961 aagtagtcgc gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa
    10021 ttgaaatttg tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa
    10081 ggagaaatta taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat
    10141 ttgtattcca gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt
    10201 gaaaaggatt ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa
    10261 ggctatgaga ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg
    10321 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta
    10381 acatttggga agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat
    10441 ggaacaattg gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg
    10501 cgaatgattc aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca
    10561 gaggtagagt tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga
    10621 actaccgtta attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa
    10681 ggaaatccta cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt
    10741 gaacatattt tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc
    10801 gttaaagcat taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga
    10861 gaaaatagcg acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct
    10921 ttctttatag ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa
    10981 gaaattggtg tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg
    11041 aaacaactcc cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg
    11101 aaatcctatt gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg
    11161 catacgcgca ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct
    11221 gttcatcggt tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa
    11281 gctatcactt tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg
    11341 aagtctcgct ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg
    11401 gcgaagcttg tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga
    11461 agtgaaacaa actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga
    11521 gactatcagt tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg
    11581 cgttcaaatt tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga
    11641 aagcggactg tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac
    11701 tttactgtat gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg
    11761 gactctgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat
    11821 cgctggtggc acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt
    11881 gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg
    11941 tacggtgatg cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt
    12001 ctatgaacgt atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg
    12061 agagaattat gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc
    12121 ttatatggag atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat
    12181 cgtgatgttg ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc
    12241 gaaatattta ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat
    12301 gagtgaactc ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca
    12361 catcaaaggt cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc
    12421 acttgtacca aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga
    12481 ttgagtgatc ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta
    12541 ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca
    12601 aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac
    12661 gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct
    12721 gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat
    12781 tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca
    12841 tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta
    12901 agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat
    12961 gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga
    13021 cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca
    13081 aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat
    13141 aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc actagggtat
    13201 agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt
    13261 aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa
    13321 aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt
    13381 aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct
    13441 tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg
    13501 tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga
    13561 taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca
    13621 tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg
    13681 cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac
    13741 tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt
    13801 attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat
    13861 cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc
    13921 ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac
    13981 cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt
    14041 ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt
    14101 agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat
    14161 ctctgtagca ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac
    14221 tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa
    14281 tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc
    14341 cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca
    14401 acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg
    14461 acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca
    14521 gttgtataag caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag
    14581 attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga
    14641 ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata
    14701 ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg
    14761 ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag
    14821 aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta
    14881 cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg
    14941 aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt
    15001 ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa
    15061 gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa
    15121 gttaacacct tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat
    15181 taaaagaccg acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac
    15241 ttagtcggcc gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca
    15301 tgaggaaaat ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt
    15361 ttgactatgt aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc
    15421 tccatttgaa atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac
    15481 agtatgtcat gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt
    15541 tagaattaaa ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta
    15601 gctggtacga ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact
    15661 tggcaatcaa tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga
    15721 aggaagaatg tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa
    15781 aggctgatga aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg
    15841 tttatttctg tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag
    15901 accaaactga gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg
    15961 cgattcaaga tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc
    16021 gttacttgcg atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg
    16081 aacaatgggg gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa
    16141 aaccgtatac cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga
    16201 aaatggctaa ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga
    16261 atgcacgctt atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa
    16321 atttgattat atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt
    16381 tggtggcagt atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga
    16441 tcgagcaatg gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DANJJW010000057        16162 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000057 DANJJW010000000
VERSION     DANJJW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            171..767
                     /locus_tag="PWS82_001744"
     CDS             171..767
                     /locus_tag="PWS82_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0906855.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(825..1307)
                     /locus_tag="PWS82_001745"
     CDS             complement(825..1307)
                     /locus_tag="PWS82_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906856.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1413..1610)
                     /locus_tag="PWS82_001746"
     CDS             complement(1413..1610)
                     /locus_tag="PWS82_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906857.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1905..2663
                     /locus_tag="PWS82_001747"
     CDS             1905..2663
                     /locus_tag="PWS82_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0906858.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2723..3151
                     /locus_tag="PWS82_001748"
     CDS             2723..3151
                     /locus_tag="PWS82_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906859.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3197..3269
                     /locus_tag="PWS82_001749"
     tRNA            3197..3269
                     /locus_tag="PWS82_001749"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3230..3232,aa:Gln,seq:ctg)
     gene            complement(3419..3790)
                     /locus_tag="PWS82_001750"
     CDS             complement(3419..3790)
                     /locus_tag="PWS82_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0906860.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4005..4190
                     /locus_tag="PWS82_001751"
     CDS             4005..4190
                     /locus_tag="PWS82_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906861.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4273..4461)
                     /locus_tag="PWS82_001752"
     CDS             complement(4273..4461)
                     /locus_tag="PWS82_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906862.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4485..4748)
                     /locus_tag="PWS82_001753"
     CDS             complement(4485..4748)
                     /locus_tag="PWS82_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906863.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4906..5163
                     /locus_tag="PWS82_001754"
     CDS             4906..5163
                     /locus_tag="PWS82_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL0906864.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5234..5425)
                     /locus_tag="PWS82_001755"
     CDS             complement(5234..5425)
                     /locus_tag="PWS82_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906865.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5566..5886)
                     /locus_tag="PWS82_001756"
     CDS             complement(5566..5886)
                     /locus_tag="PWS82_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906866.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5909..6343)
                     /locus_tag="PWS82_001757"
     CDS             complement(5909..6343)
                     /locus_tag="PWS82_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0906867.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6678..7316
                     /locus_tag="PWS82_001758"
     CDS             6678..7316
                     /locus_tag="PWS82_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0906868.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7640..7723)
                     /locus_tag="PWS82_001759"
     tRNA            complement(7640..7723)
                     /locus_tag="PWS82_001759"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7687..7689),aa:Leu,seq:cag)
     gene            8251..8751
                     /locus_tag="PWS82_001760"
     CDS             8251..8751
                     /locus_tag="PWS82_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0906869.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8941..9285)
                     /locus_tag="PWS82_001761"
     CDS             complement(8941..9285)
                     /locus_tag="PWS82_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL0906870.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9439..10359)
                     /locus_tag="PWS82_001762"
     CDS             complement(9439..10359)
                     /locus_tag="PWS82_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0906871.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10546..11037
                     /locus_tag="PWS82_001763"
     CDS             10546..11037
                     /locus_tag="PWS82_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL0906872.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(11061..13436)
                     /locus_tag="PWS82_001764"
     CDS             complement(11061..13436)
                     /locus_tag="PWS82_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL0906873.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13762..14388
                     /locus_tag="PWS82_001765"
     CDS             13762..14388
                     /locus_tag="PWS82_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0906874.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14411..15115
                     /locus_tag="PWS82_001766"
     CDS             14411..15115
                     /locus_tag="PWS82_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0906875.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15307..16140
                     /locus_tag="PWS82_001767"
     CDS             15307..16140
                     /locus_tag="PWS82_001767"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL0906876.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         4961 a         3143 c         2711 g         5347 t
ORIGIN      
        1 gcgataacta agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt
       61 cgcttatttt ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt
      121 cgtaatcaat taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt
      181 ttactgacac tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt
      241 ctaatgaaga attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca
      301 atgctcaatc aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa
      361 tcactgaaga agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca
      421 aattagctgg tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg
      481 taaaaggtct acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac
      541 aatcaaacgg aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg
      601 caaacttgca acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc
      661 cttcaaactg gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg
      721 aaaaagaata catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat
      781 aaaaaacaga ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc
      841 ataatatcaa tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct
      901 aatgatgcag aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt
      961 acgattttag aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca
     1021 ccttttttgt caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac
     1081 aaaaaactaa taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga
     1141 tgactgatga gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc
     1201 catcgatttc gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac
     1261 atcacaacaa cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc
     1321 gctttttctt ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga
     1381 cttgcagcac gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca
     1441 accatgcttg ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg
     1501 accacggcgc agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca
     1561 attggtttgc atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc
     1621 ctaaactgct ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat
     1681 tgatttttat ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt
     1741 tcacaattaa aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc
     1801 cttaaaaact aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata
     1861 catttataat aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt
     1921 tcaaaaaact attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt
     1981 acctacatat ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa
     2041 aaacgaaaaa gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt
     2101 ttgattctcc ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt
     2161 tttatggtta tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt
     2221 tcaatgcaga tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg
     2281 gagactgggc tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc
     2341 aaacggatta tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac
     2401 tatctaaagg aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa
     2461 ctgattttat gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg
     2521 cttgtgtagt cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa
     2581 aaaatcgagc agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga
     2641 tgctaacttt ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa
     2701 acctttgaag ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga
     2761 aattccgatt atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa
     2821 tgagtggaac gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg
     2881 tcaatctctt gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt
     2941 tctaattgct cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta
     3001 tcatattatg aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc
     3061 agaactgatt gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt
     3121 aaagcaacaa ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat
     3181 tattttgttg aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc
     3241 aattgtaggt tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc
     3301 tgtaaaagaa caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag
     3361 tttacataat taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt
     3421 acccaataat tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt
     3481 ctatgtgtgc tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac
     3541 cattaaattc aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg
     3601 ctgtaccata ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt
     3661 caactaatgg atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat
     3721 aatatttcag tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa
     3781 tcactttcat ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt
     3841 ccatccgtcc caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt
     3901 tagtaaaaat gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt
     3961 tgtccatgta aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat
     4021 tatttcaaaa agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta
     4081 tcgaagaaca taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag
     4141 atgtaaatat cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt
     4201 cacttttgta aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct
     4261 gaatacttca tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag
     4321 tgaggctttt cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc
     4381 cattaattct tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat
     4441 aagttgttca tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa
     4501 ataggttcat atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata
     4561 attttttcta ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct
     4621 ttctttgcta aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt
     4681 acaacatagt aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca
     4741 tgatccatga aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt
     4801 tcaaaatcga tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca
     4861 aagtgttatt ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt
     4921 caatttgttt tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa
     4981 tggacaaaaa agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat
     5041 caaatttttt cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa
     5101 ttgacgcttt cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat
     5161 tgaatccctc gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct
     5221 ttaatcgttt tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc
     5281 cttgttttaa ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt
     5341 tcttttcgat caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt
     5401 cctgtaatac ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt
     5461 tttatttaag ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac
     5521 tcctcgaact aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat
     5581 ttcattctgc tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata
     5641 atcatagctg tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc
     5701 gttagctgtg acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac
     5761 accttttttt cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc
     5821 gtcttcaata attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg
     5881 aaccattttc tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat
     5941 gtgatacgac ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg
     6001 tcgctcccat aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat
     6061 ttcttggatc tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat
     6121 cattgatttt tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg
     6181 aaaaggcata ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt
     6241 gggcttcatt gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg
     6301 atccgtcaac tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt
     6361 tcttttattg taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat
     6421 gatgaacaaa agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct
     6481 gttctaatcc cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga
     6541 acatcattct gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat
     6601 gaaatatttt cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg
     6661 aataaaggag atttttcatg acatcactta aaactttact ttttggaaca actttggctg
     6721 ccggcgctgc attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat
     6781 caggtgatac tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg
     6841 ccattgcaga atcaaattca atttctgata ttaacctgat ctactcagga caacaattaa
     6901 caatcccaac agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag
     6961 caccagtcca agctgaacct gttcaagctg aacaacctgt tgtacaagaa acggttcaaa
     7021 cggaaacaca agcagcgcct gttgctgaaa cacaacctgc tcctgctgta acagaaactg
     7081 ctgctactcc agcaagtaca agctctgcaa aagaatggat cgcacaaaaa gaatcaagcg
     7141 gttcttacac agcaacgaac ggacgttaca tcggccgtta ccaattagat tcttcttact
     7201 taaatggtga ctactctgct gcaaaccaag aaagagtagc agaacaatat gttacttcac
     7261 gttatggttc ttgggaagcg gcgaaagcat tctgggaagc aaacggttgg tactaagatt
     7321 cattatttga acgtttaaac acattcaaaa tatagtaaga ggtcatgcga tttattcttt
     7381 cgcatgacct ctttttgagt ttaagtttag tctttattcc ttaactatcg tttgccagta
     7441 tttcttgtat cagaatctat aaatctacta tttgaatcaa tctattaggt tttaaactgt
     7501 cccaccttca cacaaatttt ttttgattta tttgaatctg gttccgttgt ttaaaatttt
     7561 tcctatattc ttttagataa tatgttattt tctttctcat cgctccaggt gtaccttctc
     7621 tttataaaaa aacaaattat gcggctaaga agactcgaac ttccacgagg tcaccctcac
     7681 tagcgcctga agctagcgcg tctgccaatt ccgccatagc cgcatcacat cttttagttt
     7741 aggattacat gtgttttttg tcaagaacat atttttgttc tttttcattt ctgtacgccc
     7801 tcccttatgc catctacaca aataattcat ccgagcaact ccatcatcta ttgagggttg
     7861 gtgaaaaaat ctgttttatc catatgatcg atttcaatgt taaacgaacg actctgggat
     7921 tagtaactat tttatttttt tcttcatttc ttttttcctc tttttctcga ttcagctatc
     7981 ttctttcatt tggatattga ttatttcttc tgttgattac tatccttctg gaattctaga
     8041 cgctgaagta attcacgttt tctttcttct agagatcata agtaacgaat gaacgaactt
     8101 caaataattt ttttattgta attcatattt gtattgttcg atgattctta atacgatatg
     8161 acacaataaa taaaaatagg catggctatt tacaacaata gtgctatagt agaaaacaaa
     8221 tgaataaggt tacttaaaga ggtgaatacg atgaaactga tcaaactatg ggaagcagat
     8281 ttaaagaaag cttatcagct acaaactagc tttggaccaa atgagagtgg atttatcaac
     8341 aatgcttatg gctatacatt tgctgaattt ttagattatg taaaaaaatg cgaaaaaacg
     8401 tctttaggaa tcgatcttcc tgagagttac gttcccgcaa ccgtctttat tttagaaagt
     8461 gatgaacatg agtatgtagg aatttttaat ttgcgccata aactgaacga aacattaaga
     8521 aacggcgctg gccatattgg ttacggcatc tcccctaatt atcgtaaaaa aggatatgcc
     8581 acacaaggat tagcattagt actacaagag gcaaaaaaaa tcggcattga tgaggcactt
     8641 ttgtctgtaa ataaaaaaaa tcttggcagt cttaaagcac aacaaaaaaa tggggctgcg
     8701 atttatcgtg aagatgagaa agaatactat acgaaaatca aactgatgta aaaaagaagg
     8761 attccaaaag gaaagggatt tgaatgtcct ttatcaatag aacacattta tttaattaac
     8821 ttataatata gcagacaaaa ttttataaac aaagaaacct tttatctctt ttcttctaga
     8881 aaggataaaa ggcttttttg tttttcctat ttgcttctca tagctagtca gccaagattt
     8941 ctatcgtatc cccaacgcaa tccgtgcata acggctcatc ttatctgtcg tccatgctgg
     9001 ataccagact agtttgactt ctgctttcgt tacttctggc acttctttta atgcgtcatg
     9061 gatagattct gttaaaatat ctgctaatgg acagcccata gttgttagag tcatcttaat
     9121 gactgtttct ccattttcag gattgaattc tatatcataa atcaagccta aattgacgat
     9181 gtcgattcct aattcaggat caatcaccat ttccaaggca gcaagaatac gttctttgat
     9241 tagttctatt tcttgtccag accgttcttg gtttgcttcg ctcatcgtac gacactcctt
     9301 aacacgattt ttatttatca tactcttttt ttagctgctt gtaaatgaga atttagaagt
     9361 aagattctcg tttaaattta taaaaatgtt cttattagat gtaatagatt aaatttattc
     9421 taaaataaac ctgattcctc attttttttg tgaacgattt tttatttctg gcaatacgcc
     9481 agttgttcga tacatttttt gttcgttttc atttgcaaac cggccaaata ctgaatgagt
     9541 tggtaaaata ttgataaatg catcgtcgtc gatttcatga acgatctgat caagatcata
     9601 catttcgtag cgggtgatga ccatcatgat catgttccct tgtttttttg aatagccacc
     9661 gacagacggc agtaatgtca ttccacgtac catccgtttt gctagttctt ctgttacctc
     9721 ttctggcttg acggtaacga tcattgctgt tactttttga tgactcgtgt ggatggcatc
     9781 aattacttga gtcattgcat aaatggaaat aatcgtatac aaggcacttt cccagttaaa
     9841 gaagaagcct gcgatgacta caatgatacc atttagaatc agcatgtagt ttccgactgt
     9901 ttttccagtt gtttgtgaaa gtacaagaga gatgatatcc atcccaccag ttgtaaagcc
     9961 catttttaaa gataatccta cgccaagacc taataaaaca ccgcctacta gggcattcat
    10021 caaaatatta tcagtgatct gtccgactgg aagcagcatc gtcatcaccg atacacatac
    10081 aacattcaca aaactaagga tcgttgctcg ttttcctact ttgagaaacc ctaatacaaa
    10141 cacagggata ttcaaaagaa aaataaatgt tcctgtgtct atcttgatat gaaaccattc
    10201 atataaaagc gaggaaatga tttgagcgat cccattcatc cctgcagaaa agacattggc
    10261 tggaatcaaa aaataattta aagcgaccgc agcggtcaat ccggttatca aaataaccac
    10321 tattttcttt aatctttcat ttttatgata cgcagtcata attttactca caagcacctc
    10381 tcccctactt tggtattcca aatgtaagaa taacagttta tctattaaaa taaaatactt
    10441 cgaactcaaa tgagaagaat tttagcaaac aggtaagaat ctgccctaat cctatgataa
    10501 gatagatgta tggtaaatat ttatatgtat atcaggaggg ttcctatggc aaaagatgct
    10561 agcttcgaca ttgtatcaga agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca
    10621 acaaaagaat tgtccaatcg ctttgacttc aaggattcta cagtagaaat caaattagag
    10681 aacagcaatc tagttgtatt gagcgatgat gatttcaaaa tcgaacagat caaagatgta
    10741 ttatttggta agctgatcaa aagaaatgtg ccaatcaaaa atatccattt ttccgaatca
    10801 gaacacgctc taggtggcaa agctcgacaa actgccgatt tggtcaacgg aatcgatcgt
    10861 gaaaacgcga agaaaatcac tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa
    10921 atccaagaag atcagattag agtgacaggt aagaaccgtg atgatctaca agctgtgatt
    10981 gctttattac gtaaacttga cttaccaatc gagttgcaat tcactaacta tcgttaatca
    11041 aaaaacgagg ctgacgtctg tcattcctcg tttttttgat ttactgcttc ttttgataac
    11101 caaaaacgct gtattccttc ttccaattcc ccaacacttc gtacttctct tgctgtttgc
    11161 caatgttgtg ggaataaacg tcgataagaa aaactagaaa atcgttgatc tgccaataca
    11221 acgattcctt gatccgccat gccacgaatc actcgaccac cagcttgcaa gactttgttc
    11281 attccaggaa gctgataagc gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat
    11341 ttgatcagtt cctgctcatg attcatctgc ggcaaaccta cacctacgat catactgccg
    11401 atcaatcggt cccctctcaa atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat
    11461 ccaatcaatg tttcagcggg atttgtttga aattttgtta aaaatcgttc gcgttcttcc
    11521 tcattcatgt ccgtttcttg gattagcaca ttgctgtcgg gatattgctg tctgaatgct
    11581 tccaccgcaa gatctaaata ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt
    11641 ccatcagcaa aagcatgaat cgtctctact aattttgcca gactgtgttc cctttttcga
    11701 taagtcgtct ctaggtaatt tgcgatgaaa acttgctggt ttccttctgg gaaaggactc
    11761 ggcagtttgt aagccagact tttttgtccc cccagtgttt cctgataata agagagtggt
    11821 gaaaaactag cagaaaaaag aatactgctt tttcctttat ccaacgactg ctcaagaaac
    11881 aagcttggat caatacaaaa ctcttttact atcagatcgc gatacgtttt ttcgactgtt
    11941 gtttcgtagt gatcatcata aaattcactg accttcaaaa aatgaagcaa atcaaaataa
    12001 taaggaagca attgttcttg tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt
    12061 ttttcgctta attgataacc tgcctttact aaagattcag ccggtgcttt ttgatgatga
    12121 taatcccaat gatcttcttt tgctatttct cggatcgaat caaattcttt taatagtttg
    12181 ttgaaacggc gatgaagttt tttgaattcc tttggtatag cttccttact tcgtttcgtt
    12241 ttttcataag atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct
    12301 tcgtcaatca aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa
    12361 acagttggat caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca
    12421 tctaatgata gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca
    12481 cgagtgatct gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa
    12541 taaccattgg catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc
    12601 ttgtctttcg ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc
    12661 atagcatctt cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct
    12721 ccctcttctt cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt
    12781 ccggtaggtg cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc
    12841 gctagttcac gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag
    12901 gcattgcgta cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc
    12961 agctttttaa aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc
    13021 acttcttctg ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct
    13081 tgactataga tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt
    13141 tctaaatctt caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct
    13201 cttttgaaaa taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact
    13261 gttgttttta aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg
    13321 tggattctcg ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca
    13381 cgtcgcaaaa taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc
    13441 accacctctt tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt
    13501 tccaaacata aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt
    13561 tttggatgaa tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc
    13621 tctttagaat atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat
    13681 aagtacctgt ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt
    13741 ggctatcaag gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa
    13801 aactgttatt tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg
    13861 gaacgaatca aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc
    13921 catactcact acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca
    13981 gtttatgtca gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga
    14041 ctcggtagac atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa
    14101 atgaaaactt atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct
    14161 ataggaagcg tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga
    14221 ggaagtatcg gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt
    14281 cggacccagc tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca
    14341 acgacgattg aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga
    14401 ggagaataaa atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa
    14461 tacaattgaa cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg
    14521 cattcattat ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga
    14581 cagtggtcgt gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga
    14641 aattccttat tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg
    14701 atatgacatg gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga
    14761 aatgtttacc acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac
    14821 cgctgggtgg gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga
    14881 tatcgcccat cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt
    14941 gacgatcgcc tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt
    15001 aaatggcagc gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa
    15061 tgatacgtct tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg
    15121 atattttaat ttttaccaga gaatcataaa aggcaaagtc tgaacgaggg ctttgtcttc
    15181 ttttttctct tcgtgtactt atctgcttta tttctcaagt ttttcttaat attaaaaaac
    15241 agattgggtt tctcaaaaaa taacggtata ctataagatg tatcttaatt gggaaggaag
    15301 gaatttatgt tttttgcaaa tattatcaaa gcagtgattc tcggtattat tgaaggaata
    15361 acggaatggc tgcccatcag tagtactggt catttgatcc tagcagatga attcatcaaa
    15421 ctggatgcca gttcacaatt tatgtcgatg ttcaatgtcg ttatccaatt aggggcgatc
    15481 ttggctgtcg tcgttcttta ttttcacaag ttaaacccat tttctccaag taaatcaacg
    15541 gtagaaaaaa aagatacgtg gacactttgg tttaaagtga tcgttgcttg cataccagcc
    15601 atcatcattg gtttgccttt ggatgattgg ttagaagcaa atttccacaa gttcttacca
    15661 gttgccattg ttttgatcgt ttacggtatt gcctttatta ttgtcgaaaa acgcaacaaa
    15721 aacaaaacac ctcgttggac ttctctgaat gactttacgt accaagcagc tcttcttgtt
    15781 gggttattcc aagtcttggc acttgttccc ggaacatccc gttctggcgc aacgatccta
    15841 ggatctatct tgattggtgc ttcgcgtttt gtcgctacag agttttcatt tttcttaggg
    15901 attcctgtca tgtttggcgc aagcggcttg aagatcgtca aatatttagc tgaagggaat
    15961 tcattcaaaa tggaagaaac tgttgttctt cttgtaggaa cattcgtatc ttttgtggtt
    16021 tccgtcttag ctatcaaatt cttactaagc tatttgaaga aaaacgactt taccgctttt
    16081 ggctggtatc gtattatcct tggtatcatt ttgattggtt attggtttat tgcaatgtag
    16141 aaaaaaccag aacacttctc tt
//
LOCUS       DANJJW010000058        15993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000058 DANJJW010000000
VERSION     DANJJW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            138..1475
                     /locus_tag="PWS82_001768"
     CDS             138..1475
                     /locus_tag="PWS82_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL0906877.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="PWS82_001769"
     CDS             1523..3652
                     /locus_tag="PWS82_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL0906878.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="PWS82_001770"
     CDS             3627..4316
                     /locus_tag="PWS82_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906879.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="PWS82_001771"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="PWS82_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="PWS82_001772"
     CDS             complement(5546..5740)
                     /locus_tag="PWS82_001772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906880.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="PWS82_001773"
     CDS             complement(5790..6230)
                     /locus_tag="PWS82_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0906881.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="PWS82_001774"
     CDS             complement(6234..7079)
                     /locus_tag="PWS82_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL0906882.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="PWS82_001775"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="PWS82_001775"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL0906883.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="PWS82_001776"
     CDS             complement(8703..9650)
                     /locus_tag="PWS82_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0906884.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="PWS82_001777"
     CDS             complement(9873..10223)
                     /locus_tag="PWS82_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0906885.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="PWS82_001778"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="PWS82_001778"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL0906886.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="PWS82_001779"
     CDS             11646..11966
                     /locus_tag="PWS82_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0906887.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="PWS82_001780"
     CDS             11988..12452
                     /locus_tag="PWS82_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0906888.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="PWS82_001781"
     CDS             12657..13262
                     /locus_tag="PWS82_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL0906889.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="PWS82_001782"
     CDS             complement(13301..14590)
                     /locus_tag="PWS82_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL0906890.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="PWS82_001783"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="PWS82_001783"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL0906891.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15993
                     /locus_tag="PWS82_001784"
                     /pseudo
     CDS             15700..15993
                     /locus_tag="PWS82_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5040 a         2942 c         3259 g         4752 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg ttt
//
LOCUS       DANJJW010000059        15947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000059 DANJJW010000000
VERSION     DANJJW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(383..805)
                     /locus_tag="PWS82_001785"
     CDS             complement(383..805)
                     /locus_tag="PWS82_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL0906892.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(819..2225)
                     /gene="atpD"
                     /locus_tag="PWS82_001786"
     CDS             complement(819..2225)
                     /gene="atpD"
                     /locus_tag="PWS82_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL0906893.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2248..3150)
                     /locus_tag="PWS82_001787"
     CDS             complement(2248..3150)
                     /locus_tag="PWS82_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL0906894.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3164..4720)
                     /locus_tag="PWS82_001788"
     CDS             complement(3164..4720)
                     /locus_tag="PWS82_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL0906895.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4743..5285)
                     /locus_tag="PWS82_001789"
     CDS             complement(4743..5285)
                     /locus_tag="PWS82_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL0906896.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5272..5796)
                     /gene="atpF"
                     /locus_tag="PWS82_001790"
     CDS             complement(5272..5796)
                     /gene="atpF"
                     /locus_tag="PWS82_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL0906897.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5887..6102)
                     /gene="atpE"
                     /locus_tag="PWS82_001791"
     CDS             complement(5887..6102)
                     /gene="atpE"
                     /locus_tag="PWS82_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL0906898.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6155..6874)
                     /gene="atpB"
                     /locus_tag="PWS82_001792"
     CDS             complement(6155..6874)
                     /gene="atpB"
                     /locus_tag="PWS82_001792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL0906899.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7176..10019)
                     /gene="rnr"
                     /locus_tag="PWS82_001793"
                     /pseudo
     CDS             complement(7176..10019)
                     /gene="rnr"
                     /locus_tag="PWS82_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10022..10777)
                     /locus_tag="PWS82_001794"
     CDS             complement(10022..10777)
                     /locus_tag="PWS82_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0906900.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10880..11116)
                     /gene="secG"
                     /locus_tag="PWS82_001795"
     CDS             complement(10880..11116)
                     /gene="secG"
                     /locus_tag="PWS82_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL0906901.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11234..11512)
                     /locus_tag="PWS82_001796"
     CDS             complement(11234..11512)
                     /locus_tag="PWS82_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0906902.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11625..12593)
                     /locus_tag="PWS82_001797"
     CDS             complement(11625..12593)
                     /locus_tag="PWS82_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0906903.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12808..12852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12876..13850
                     /locus_tag="PWS82_001798"
     CDS             12876..13850
                     /locus_tag="PWS82_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL0906904.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13855..14430
                     /locus_tag="PWS82_001799"
     CDS             13855..14430
                     /locus_tag="PWS82_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0906905.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14497..15636)
                     /locus_tag="PWS82_001800"
     CDS             complement(14497..15636)
                     /locus_tag="PWS82_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL0906906.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3442 c         2855 g         5294 t
ORIGIN      
        1 gccagaatgt ggttcattct gactcagatt tgattttgca caactcatca gtccttattg
       61 acaaagagcc caaaagtctt gtcgcagtcc cttattccaa ataaacggtg ttcaatcatc
      121 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc catttgtttc
      181 tctaatactc aggacaaaac gctgatttca caacctccga tatacggtgt tccagaagta
      241 gcttcttcga aaaataagct gagatttcac aaaaatttga gaagcaattt tcgtaaattc
      301 cttcttatgt cttgaagctg accacttctg tcataacctc tttttactgt tcttaaacac
      361 ggataaacat ccgcatctca ttttatgagt gcttagatac tttgatacga ttgattgctc
      421 ggtgcaacgc aacagttgcc cgtttcaatt cgttcgtatc ttgttttgct tttgcttctt
      481 cgatttggcg ttctgcgcgt tgttttgcac gttccgcacg agatacatcg atatcacgtt
      541 cacgttccgc actatcagca acgattgaaa caacattatc acgaacttcc atgattccgc
      601 cattaacagc gatccaatcg acgtgggtat ctgaatctgt tcgttttaca cgaacttcat
      661 caatcgtcaa tggaacgatg attggtgcat gtttaggcaa aatcccaagc tcaccagctg
      721 tcgtacgtgc aacgacgata gctgcatggt gatcatagac caaaccggca ggagttacca
      781 catttactgt taaatattga tccatggggc acccctttct agtagcctag tgttttcgct
      841 ttttctacga cgtcttcgat tcggccaaca cttcggaaag cttcttcagg aagatcatcg
      901 tatttgcctt ccaagatttc gcggaatcct ttcactgttt cttctacagg gacatatgaa
      961 cctggaagac cagtaaattg ttcagccacg ttaaagtttt gtgacaagaa gaattggact
     1021 ctgcgggcac gagaaactaa cactttttct tgatcggata attcatccat acctaagata
     1081 gcaatgatat cttgtaattc acgatatcgt tgcaatacgt gttggacttc tgttgctact
     1141 ttataatgtt cttctcccac gatttcaggt gctaaagcac ttgaagaaga agccaatgga
     1201 tctaccgctg gataaatccc ttgttctgtt aatttacgtt ccaagttggt tgtcgcatcc
     1261 aaatgggcga atgctgttgc cggcgccggg tcagtgtagt catcggctgg cacatagatt
     1321 gcttggatcg atgtgatcga accttttttc gtagatgtga tacgttcttg caattgaccc
     1381 atttctgttg ccaatgttgg ttgataacca accgcagaag gcatccgtcc taacaaggct
     1441 gatacttcag atccagcttg agtgaaacgg aaaatgttgt cgataaacaa cagtacatct
     1501 tgaccttcta catcacggaa atattccgca atcgtcaaac cagtcaaagc aacacgcata
     1561 cgtgcacctg gtggttcgtt catttgtccg aacaccatgg ctgttttttc gataactcct
     1621 gaatctttca tttcataata caagtcatta ccttcacgag tacgttcacc tacaccggta
     1681 aatacagaaa taccaccatg ttcttgggcg atattatgga tcagttcttg gatcagtacg
     1741 gttttaccaa caccggcacc accgaatagt ccgactttcc caccttttaa atatggggca
     1801 agcaaatcga taactttgat ccctgtttcc aaaatttctg tacttgtact taattcgtca
     1861 aaggctggtg cttttttatg aatttcgctt ctttccgcat cttctgggaa aggtgtttct
     1921 aaatcgatcg tatctcctag tacgttgaat acgcgaccta atgtttcttt acctacagga
     1981 acagagattg ctttgcctgt atcaatgact tccattcctc tttgtaaacc atctgttgat
     2041 tccatcgcaa tggtccggat cactccgtcc cctaattcta acgctgcttc aagaacaact
     2101 tttgatttat tttcgtcatt tttataaaca actaatgcgt tgttgatatc tggtaaggat
     2161 tggtctaaag aaaattccac gtcgacaacg ggaccgatta cttgaacaat cttgcctgaa
     2221 ctcatctttt ttcctccaat ctcaatacta ttctagtgcc gaagctccgg caacaatttc
     2281 ggtaatttct tgggtaatag ccccttgtct tgcacggttg taagaaaccg tcagctcatc
     2341 aatgatatta gcagcattat cagttgctgt tttcattgct gtcataccag cagcatgttc
     2401 tgctgttttg gcgtcaacga tcgcaccgta gatcaaactt tcagcatact gaggcaataa
     2461 ctgagacaag atttcttctt ttgaaggttc aaaaatatat tcttgttcga aagtagttgc
     2521 ttcttctgga tctaagtctg agattggcag cattttttct acacgaaact gactagttag
     2581 cgaattgata tgatggttat aacatacgta taattcatcg aatacttcgt tttggtacat
     2641 agttgttgcc atatttacga ttttgcggac ttcatcaaag cttggctgat cagatagatt
     2701 gcgcagctca tatgctagat tgataccgcg tgctttaaag aagtctgcac cagttccgcc
     2761 aattgcgatc atcacgtaat catcgggtga ttggtggtct tcttctaaaa tagacatcgt
     2821 ttgtttcaat atcgagctgt tatatccacc gactaaaccg ccatctgcag tgatgacgat
     2881 ataacccgtt ttcttaacag gacggctgag caacatgctg ttgtaattga tatcccctct
     2941 aggattttct gaggcaatat catttaactg agtagcagtc aaatgcgtaa cgatttctct
     3001 tacttttgtt gcataaacct gaaacttctg tgaagaagct tcagattttg tcaacttaga
     3061 agcagacacc atctgcattg cccgcgtgat ttggctcgtt tttttcgttg aagcgatacg
     3121 tgtcttgatt tcatttaatg aagcacccat tcatctcacc tcactatgca ttttgaatgg
     3181 atttcaattt atcttctgta gaggaattat ttccttttgt agctaagaac atgtctttat
     3241 attcctgaat cgcactgttc aacgcttctt cttctggaag atcttttgtt gtacggattg
     3301 tttcaaagat atctgaatga ttggtatcca gatattcgaa taattcatgt tcgaagtcta
     3361 aaatactgtc tacagggata ctatctaaga atccatgtgt caaagcatac aaaatcacta
     3421 cttgtttttc aacagctagc ggtgcatgca atttttgttt caagatttct actgtacggc
     3481 gtccacgatt tagttttgct tgtgttgctg catccaagtc agaaccgaat tgtgtaaatg
     3541 cttctaattc acggtaactt gctaagtcta gacgtaaagt cccagcaact tttttcattg
     3601 ctttgatttg cgcagaaccc cctacacgag atacagatag tccggcatca accgctggac
     3661 gagtacctgc gtagaacaag tcgctttcta agaagatttg tccatcagtg atcgaaataa
     3721 cgtttgttgg gatataagca gagatatctc cagcttgtgt ttcaacaaat ggtaaggcag
     3781 tcattgaacc gccacctaaa tcatcactta attttgctgc acgttccaat aagcgtgagt
     3841 gtaagtagaa aacatcccct ggataagctt cacgacctgg tggacggcga agcagcaagg
     3901 aaagttcacg ataagcaaca gcttgttttg aaagatcatc aaaaatgatc aagacatgtt
     3961 tcccgttgta catgaactct tcacccattg cagtaccagc atatggtgcg atataaagca
     4021 acggcgctgg ttgagacgca ccggcattca caacgattgt gtaatccatt gcgccatatt
     4081 ttttcaatgt ttcaacttgt gtacgaactg tagaatcttt ttgtccgatt gctacataga
     4141 tacagatcat atcttgacct ttttggttga tgatcgtatc gatcgcaatg gaagttttcc
     4201 ctgttttacg gtcaccgata actaattcac gttggccgcg tccgattggt acaagagcgt
     4261 cgattgcttt tagacctgtt tgcattggtt cgttaacaga tttacgttgc ataacgcctg
     4321 gtgccattgc ttctactgga cgagctttgt cagtaacgat ttcacctagt ccgtcaattg
     4381 gctgacctag cggattaact acccgtccaa tcaaggcctc tccaactgga acttccatga
     4441 tctttcctgt tcgttttact ttatctcctt cacgaatgga ctcgaaatcg ccaaggataa
     4501 tgatacctac atcatttgat tctaagtttt gcgccattcc gtatgagcca tttgaaaatt
     4561 caagcaactc accactcatc gcgttttcta aaccatgtgc acgagcgatc ccgtcaccta
     4621 cataagttac ggtaccgatt tcttcgactg aaagcacgtt ttgataattt tcaatttgtt
     4681 ctttaatcaa ggcactaatt tcttctgctt tgatggccat tcgattcacc tacctctttg
     4741 tactatctat ttaattgatt acgcatattt tctaattgcg tgcgaatgct tccgtcgatt
     4801 actcgatggt tagcttcaac aatcgcgcca ccaataatag aagaatcaac gatttgtttt
     4861 aattcgactg attgatagtt cattgttttt gcaacattct tttctaattg tattcgttgt
     4921 tcgtctgaaa gtggaactgc tgttgtgaca cttcccaaca gcaaaccttg atgttcatca
     4981 tatcgacgtt catattcttc gatcatgaac aaaagatcat ccattcggtt atacgcataa
     5041 acaacttcta aaaagttttt aacgattcca tcatagccac taacaagctt gtccatgatt
     5101 tctcgttttt catgaggttc tagacgaaca tcacttaaca tattacctaa tccaggtact
     5161 tgttcataga tttgacgtaa gcttacgaga tcttgataaa tttcttctgc tttatttgaa
     5221 tcgatcgcca gttcaaataa agctttcccg taacgtcttc ctactgtata tttatctagt
     5281 ttcatttgaa gaacctagac cttcgatata ttgattgatt aatgattcgt gcatttctgg
     5341 agataattct ttgttcaaga ttttttccgc gatttgaaga gatagttctg caacatcgtc
     5401 ttttacagag ttcagcgctg aatcacgttc tacagtgata tcagcttggg ctttactttt
     5461 tagacgagct gcttcttctt gagcatcctt caaaatattt tggcggctta attctccact
     5521 ttctttcgca tttttgatga tatcagctgc atcagaacga gaggctaaca attgttgttc
     5581 gcgttcttgt tccattttcg ctgagttgat gcgagattgt tctgcagaat ccaaatcatt
     5641 ggcgatcttg tcttcacgtt ttttcaaaat atcgctgatt ggtccccaag caaaatgctt
     5701 caagagagcc agtagtatca aaaatgagcc gctgacaacg atgacatttc ctaacatcgc
     5761 attgccaact tctgcaattg ccaattgatt cagcataggc gagccttcct ttctttgaag
     5821 tacttctagt tttcatgtaa aaaagcagta acaaaccaaa ttgtttctgc cttaatactg
     5881 attcaattaa acaccaaaaa ctagaatcaa ggcgacaact acccctaaga taggaacagc
     5941 ttcgactaga gctaccccga taaacattgt tgtacggaat tgaccagaca tttcaggttg
     6001 gcgagccatt gattcgattg ttttagagat aacttgaccg ttaccataac cggctccgat
     6061 tgctgctccc atgattgcga ttgctgctgc gatataattc ataattgttt tcctcctaaa
     6121 ttagataaat agtagtttgt ttttaaaacg aatattattc ttccacttca atcttatggc
     6181 tcatataaac catagataaa gtaacgaaga caaatgcttg gatgcttccg ataaacaagg
     6241 agaatgcgat ccagaccatc tctaatggaa ttgctaacgg caatgagaac cagcctaagc
     6301 ttattaccat ttttgcaatc aaggttagca aaacttcccc cgcataaata ttcccataaa
     6361 gacgtaaagc aagtgttaat aagtttgtaa attcttcaat cagtttcatc ggaaacataa
     6421 aactatatgg tttcaagaac gaatttacga aatatccttt gaagcctaac tttttcaccc
     6481 caaataagtg agtaagtaag atcattataa atgccaaagt taaagtaaca aacgggtctg
     6541 ctgtcgggct tttccaaagt gttgtttcgt taggtaaaac gatttttgtt actaaaccga
     6601 tgttgtttgc aacaaaaaca aataaaaaca atgtaaatgc caacaaatgg aaattgctca
     6661 attcttttct aggcagatta tctgttacga ttcctcgact aaaatcaatg acccattcta
     6721 tcgcattttg tttgcctgtt ggtttcaact taagatttct tgtaaaaaag taagctaaga
     6781 agaaaacaat caaacaggtc agtaacacca ttatacaaac tgtaccatca aaccaaattg
     6841 gtccaatgtg gaacgttagc gagcgttcat ccaatccatt tcacctcttt tccccgaaat
     6901 tcacataaaa tcaattcatg tgcgtatctg gttttttcca atcggtttct aaccacacgg
     6961 tatcatacca ccatgaaaca gaaaattcaa aacattttca tgaaaattag acactttttt
     7021 atttcagtca aaaacgaagc gaatttttct ttatacacgg tgctcctgat ctaagaaaaa
     7081 acctatgata atcatgtttt atcagattaa atgctaacaa attttatcat taagtaaaag
     7141 caaaagcacc gaatatgcga aaaaggataa tgaaattatc tagagaaatt ttttaatgtt
     7201 cgatcaattt gtcgatcgat atcttttcgt ttcaaatctt cccgtttatc ataagatttt
     7261 ttccctttcg ccagaccaat caataccttg gcatagccgt tacgaatata gacttttaaa
     7321 ggaacaacag tgattccagt atttttcaat tcattttcaa gccggatgat ttgttttttg
     7381 tgtaataata actttctcgt gcgcaacgga tcatgattaa aaatatttcc ttgttcataa
     7441 ggactgatat gaacattatg caagaaagct tctccgttgc ggacgcgaat aaatccatct
     7501 tttagattga tccggctgtt tcgtatcgac ttgatctcgg ttccttgcaa gaccatccct
     7561 gcttccatcg tgtcgatgat cgaataatca tggcgagctt tcttgttttg tgcaattaat
     7621 tttccctcgc cttttggcat agctgtttcc tccaactcat tttatttttt ctttttcttc
     7681 tttttaacac ctttgtaaaa tggcttcttc ccttttttct taccgaacgt ttgcttacta
     7741 tcgctatatc gtttgttctt cttgccattt ctttctgggc gttttcctgc ttgttttcct
     7801 tcgcgttttc ctgattttcc atttcgtgtt tttggccgtt ccaacgctgt aacctcttct
     7861 gcggaaacaa gttcaaaatc tactgctcgt tcatctggat cagctttgac tacttttact
     7921 cgcactttct gtccgatttt aaaagtcaga ccagttcttt cacctacaag agctaaatga
     7981 ttttcaacaa aatggaagta gtcctgtttc aattcattga tatggatcaa tccctctact
     8041 gtattcggca gttcgataaa caggccgaat ttcacaacag agctgatgat cccatcgaat
     8101 tcttccccca ctttgtccat catgaattct gctttcttca tggaatctac ttcacgttct
     8161 gtttctactg cacgacgttc catcttagaa ctatgatctg caatttcagg aagttccgta
     8221 gcccacgaac tttgattgct ttcactttta tcttcactgt aagaacggat cagacgatgg
     8281 acgattagat cgggatagcg tcgaatagga gatgtaaagt gtgtgtagta atctgccgct
     8341 aagccataat gcccaaaatt gtcttctgaa tagcgtgctt gttgcatgct tcttagcaac
     8401 atagtattga taactgcagc ttctggtttg tcttctactc cttcgatcac atgctgtaaa
     8461 tcttttggtg tgatcgttcc tttcgttcct cggaccagaa tgcccaaagc tgaagcaaaa
     8521 tcaaagaagc gctgcatttt ttcttctttt ggctgttcat ggattcggta aataaacggc
     8581 agatttaatt tattataatg ttcagctacc gtttcattgg ccgcaagcat gaaagattcg
     8641 atcaagcgtt caccaattcc tctgcttctt agctggatat catttgggtg tccttcagca
     8701 tcaacaagga tttttgcttc gcgatcctca aaagaaatcg cgccacgttt gattcgcatc
     8761 gtttcaagga ttcgatgcaa ttcacccatt tcttggaaca taggtacgag ctgagcatag
     8821 cgttgcatcg tttcttcgtc ttgatcttct aagatttgat tgacagctgt gtaggtcatc
     8881 cgttctgttg tttggatcac acttgggaaa atatcatgag agactacttg tccatttgga
     8941 tcaatctcca tttcgcaact catggtcagc cttggaacat ggggattcag tgaacagata
     9001 ccattcgata aacgttgcgg gatcatcgga atcactcgat cagtcaaata tacacttgtc
     9061 cctctttcat atgcttctct gtccaattga ctgccttgtg tcacatagta agaaacatct
     9121 gcaatgtgta ctccaagaaa ataattaccg ttatctaatt ttttaaccgt aactgcatca
     9181 tctagatctt ttgcatcttc tccatcaatc gtaacaatga gctgatttct taaatctcta
     9241 cgtccagtta tatccttttc gctgatttga tctggaactt catctgcttc agacaataca
     9301 tcatcaggaa atttcgtagg gataccatta gccacgacaa ttgagaggat atccatgcca
     9361 gggtcattct tatgaccaat cacttgcttg attatccctt ctaaactagt cgcataccct
     9421 ttttcagggt aatgggtcag ttcgatcagc acaatacttc cgtcaactgg acgaactccc
     9481 tctgctgctg catatacttt aaattggttg agctttctat ctttcgggat aacaacgccg
     9541 taaagatccg tttcttgtat ttcttcttct gaataagcaa cgaattcccc tgctaactga
     9601 gtagtcgccc ggtctcgaat ctcgactact ttcccctctg ctccgcgatc catatctgta
     9661 tttgctcgct tcatgatatc gattgctacg gtgtctccat ccattgcaaa accagtcgct
     9721 tcttttggga tatagatgtc atcttcttcg ggatcaatcg ttacaaatcc aaatcctcgt
     9781 tcatttgctc gaaatgttcc ttcgatcaag attggtttta aaggaagttt cacttttcct
     9841 tttttattga aaactatact ctgttctctt tccatttgag caatcgtttg tactagtaat
     9901 ttaaaatcat cgcttttttg gaaccctaaa ccttcagcga tttcttccat agaaaaactg
     9961 cgtttgcgac tgctttccat gaaaaagaga attttctctt ttatcgtttc ttttgtcata
    10021 tttattcctc attccaaggt aatttttcta aaaattccaa gacgtctttt tctaattcgc
    10081 gtcttgctgt accaactgta atcacatgac cactttcagg ataccatcga agtgtcactc
    10141 gctgacggct caacttgcgt gcagtttcaa atacgccatt agggtcgatc atttcatctt
    10201 tcccagcttg agctaaaaat atcggctgtt ggatatctga caacctactt tctacgattg
    10261 ctgcctgatc ttgtatatcc attaattgtt gttcagctaa cgagcggtaa gcaactgctt
    10321 tttcattgat ttctttctct gatttacctg ctgtcttcag cacttgacgt acgtattttt
    10381 caaagttttc aggcacgttt gtttttacag gagacaaagg cgaacagaaa aaaccgccgc
    10441 caatcactga ttcttcttct agtgctcgta cagcaaagat gccgcccatt gataatccta
    10501 atacggcaat atcagaaaac tgctcagact gtaaaaaatg gatggctttt ttagtgtctg
    10561 cccaccagct ttctgctttt tcttgcaaaa tatcatatgg ctccattgtt ccgtgtcctt
    10621 taaaaagcgg cgcataaact gtataatctg ctttttccaa aaatcgtgcg agcattcgaa
    10681 catcgtttgg acttcctgag taagcgtgaa gcagcaaaac cgctcgcttt ccatgttttg
    10741 cataaagggg ttttggtaat tgtctcactc gtctcacctc tttcgcctca ttttaccata
    10801 gtttctttca taacttcttc ttaagcgttc tctttgcaat aaaaaaagcc gcccgccgtt
    10861 ttgcgagagg cttattcaac tacttcgaag ataaatatgc taggacaaat aataagatca
    10921 tccagatagc tcccataact gcagttgatc gctgcatgac agcttcaaag ccgcgagctt
    10981 tttgtttgcc gaatagttga tccgcaccgc cggtaaatgc actcgctgca ctattttgct
    11041 tgcttggctg catcataatc gcaatgacca taacaatcga taaaacgatc acgattccca
    11101 aaattaaatt atacatatcg cagcctcctt cccaaaagat atcgtctctt taactttatc
    11161 atagataaaa gtccgtgaca actctatata aatggaataa cagctgaaag ttcaaaactt
    11221 tcagctgtta ttcttatgac tgacgtaaga cgtcttgata ttctggtttc ttttcaaact
    11281 ctttttttgc ataagggcat aaaggaatga tctttttccc gtctcgtctt gctaattcta
    11341 ctccagtata cactagcttt tcagctaact tttgaccacg atatgctgga tctacaaatg
    11401 tatggtcaat gatcatgatg tctgggcccg catctgacca tgtcatttct ccgatctctt
    11461 gttgttcatc attgaataaa gcaagacgat ttttttcttc tttgatttcc atatgccgca
    11521 ctcctcaaat aactgatatt tttttattcc taccttattt tagcagtctc ctgatctata
    11581 aacaattatt tatcctcgaa ttacttacaa aaaagaagag aaaattaatt catcgttatt
    11641 ctttgttcaa taaatgaaaa aatcactgtc caataggttt tcggatcttt catcgatgct
    11701 tccaagtgtt ctgcttctgg aacaagcagc agtgctttat cccctgccgt agcattttga
    11761 atcgtgtacg ctgcttctgt agggacaaaa tgatctcttt caccatgaat gaccaataaa
    11821 ggcagatggt tgctgccaag ctgtctcgta gctgatgctt gctttaagga gtaaccggcg
    11881 tatttctggg cataatgatt cgcatatctc atgaccattt ttttaggaaa gacagtgatt
    11941 ttacttaaca tatatcggaa ttctgcataa acagaaacat atccgctgtc taaaataaat
    12001 cctttgaccg ctgatggaag tttttcacca cttgccatca tgatcgtaga agctcccatt
    12061 gatccgccgt gtaaaataat ggacgcattc ggctgttcat ctaagatgag ttggatccag
    12121 gcaattaaat ctagccgatc tagccatccc attccgatga tttctccttc actttctccg
    12181 tgcgcacgta aatcaggtac aagaacgttg aatcctttct ctgcatatct tttacctata
    12241 tgagacatgt ctcttttccc tgtgcttcgg taatcatgta cgcaaatgac ccatttattc
    12301 tgcttatttg gatggatgaa catctgacca gctagtttca acccgtcata acttttgatt
    12361 gttttgggtg ttccctgctc ccaaaagctt tcacactctt cttctaattt ttgtcgtttt
    12421 ttttctactg acgtgactgc agcagggtcc atcaaacgat gaccagtgtc ttcataccag
    12481 tcatatcctc tctgtactcc cgctcttact aatttctttg cataaaaata agaaagaaaa
    12541 tggtaactga tccctataat tatcagaata agaatgaaat aaaaaataac cattgtacac
    12601 ccgccgcttt ctgccaaact ataatctaac tattatccaa tgtaccgatc cataggattc
    12661 tttattttcg gcacacgtaa attccatttc tttttcattg tatcatttac ttaaagttct
    12721 cctcaataga tttacttgta agagagcatt tacattcgca ataaaagcca gtatactaat
    12781 ttcgtgcatt ataatcaaga cgctcgtctt ggactggttc ggaaacttcc cagaataaaa
    12841 aactaagtat ctctaaagaa aggagattgg aaataatgcg aaaagtaatt attgattgcg
    12901 atccagggat cgacgacact ttagctttat cattagctgt caaatcccct gatatcgaag
    12961 tagttgctat taccgtagtt tgtgggaatg tccctgcaga tataggtacg cagaatgtgc
    13021 tgaaatgcct ggaacgctgt gatcgtttag acattcctgt ttatcaagga atggaaaaac
    13081 ctttgaagca acctttcatc agtgcacaag acactcatgg tatggatggt ttaggggaaa
    13141 caaatttccc gatgatcttg agaaaacagg cagaacctct ccatgccgtt gattttttgg
    13201 ctgattattt caaagaaaag acaaatacat cagtcattgc tttgggtcct ttgaccaata
    13261 tcgcttttgc tttgaaagtc aacccaaata ttgggaaaca tatggatcgt tttgtctcga
    13321 tgggtggaac ttacaaaagc catggtaact gttcgccagt tgctgaatac aattactggt
    13381 gtgatcctga agcagcttct tatgtattcg agaatctcaa acaaacgata gaaatggttg
    13441 ggttagatgt gacaagagaa atcgtcttga caccgactat cctcgaatac tgctgtcaaa
    13501 tgaatcctga agagggagag tatctaaaag cgatcactcg tttttatttt gacttccact
    13561 ggaaacaaga aagaatttta ggatgtgtca tcaatgatcc gttagctgtt gcttatttta
    13621 tcgagggggc catctgtgaa ggtttcaaaa gcttcactgc tgtggaaact caaggaataa
    13681 gtcgtggaca aacattggtt gatcgttatg aattttggca gaaacctgct aacagtaaaa
    13741 ttatgacaaa agtagatact cgtctttttt tccgaaagtt tttaaccgtt cttttaaatg
    13801 cacaagaaga aacgattatg aaagatttgg aaaggttaaa gatgggctaa caaaatgaat
    13861 aaaaacaaaa tggcaactcg cgtacttaca gtgatggctt tgtgtattgg gatcaattat
    13921 attggaggaa cgattgcttt atggcttcgt ttgccaatct atctcgattc tatcggaacg
    13981 atttttgctg gtgcattgct tgggcctgta cctggaatgc tgacaggatt gtccagcggt
    14041 attttaagtg gagtgactac agatattttt tctctgtatt attcgccaat ccaaattctc
    14101 acaggcttgc ttgctggatt gattttgcca agatatctcg gcaaagataa aaacagcttg
    14161 aaactgttat tactcgcttt gattttgtcg gtacctggaa cgatcgtttc ttcgatcatt
    14221 accgttcagt tatttggcgg gatcacctcc tcaggatcca gtatgatcgt tcagctatta
    14281 catgggttag gtttaagtca gacaacaagt gtgatcttgg tacaggcagg tacagactac
    14341 cttgatcgtt tgttgtctat cgtcgttgtt tcattagtca tcactatgtt gccacaacgc
    14401 ttgatatatt caaaaagaaa aataggctga ataaacagag ctggattagc aaaataatgc
    14461 taatctggct ctgttttttc atttattaaa aaaatcttac gctaagtagg ataaaataat
    14521 tccttgatat ttttcaatca tatccaaata attatcgatt tcaacatact catctacttg
    14581 atgaggcaaa gtaacgacac caggcccaaa gacgacaaag tcgaaggaat ggttagcttt
    14641 cgtaaattcg gctgcatccg ttgttgctgc cgctccaact aaaggtaatg gctggctgaa
    14701 ttgttgctga atgcaatgaa tcaaaggaga atctggatca gctttgacag gtattttgtt
    14761 gtaatcgatc atcaattcca aatgatagtc tgtctcttga ttcaattcat tgacgattga
    14821 ttgcagcaat gcaatgattt tatcatttgg gtattctgga atcgatcgaa tgttcccttg
    14881 gagctgcgca tggcttggga tgctgttgac ttgattcccg ccactgatca atgtaacatt
    14941 atggattgtt tttcctagga caggattctc gattgtttca gcgaggtgat tcatttcagc
    15001 atttgctttc gtaatgaatt catttaggtg attgattgca ttataacctt ggtctggcat
    15061 agagctatgc gcttcttttc cgtgcgaggt gactgtatag ttgattgatc ccatgtgcgt
    15121 atacatcaaa ctgtaattag ttggctctcc gatgatcaag gcatctaagt catctacata
    15181 gcctgctttc gtgagctgtt ccccacctag ttcacccact tcttctccga cagtggctaa
    15241 taatttgact gttccgttaa atggttttcc tgactctttc agttcgatca tggcaatcac
    15301 cattgctgcc aagccactct tcatatctgt agcaccacgt ccgtacaaac ggttcccatg
    15361 gatttctgct gcaaagggtg cataggtcca agacgattca tcaccagccg ccacgacatc
    15421 catatgccca gataggccta acacttttcc tgattggccc ttttggtaac gagcaatcaa
    15481 attatctcta ccatctctgt aagaaacaat ctctcctgtt atgtcatgct ttgccaaaag
    15541 cttattcaaa taggctgcta cttcaccttc gtttccattc actgatttaa ttcggatgat
    15601 ctcttgtaaa atcgcgattt tttcttcttt tttcatctaa tcctctccct tgccatttct
    15661 ttttattgta tttgagtgtg ctcgaatttt caaataggag caaccaaagt aaaaatgaag
    15721 aaagagagcc actttatgaa aaatatgctg ctacttccct cctattttat ttatacgaga
    15781 ggacttgctc atagattcct attctactgt tctatcctta aaagctctta tatgatgcta
    15841 gttattgtta gctttccttt ttcaaggatg atcttttctt gtaccattct ttgtaaacat
    15901 catatacaga agctagttgc aaaaaaaact ctctacctgc tgatatt
//
LOCUS       DANJJW010000060        15693 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000060 DANJJW010000000
VERSION     DANJJW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(12..1535)
                     /locus_tag="PWS82_001801"
     CDS             complement(12..1535)
                     /locus_tag="PWS82_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0906907.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="PWS82_001802"
     CDS             1787..2968
                     /locus_tag="PWS82_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0906908.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWS82_001803"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWS82_001803"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL0906909.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="PWS82_001804"
     CDS             complement(5053..7764)
                     /locus_tag="PWS82_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906910.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="PWS82_001805"
     CDS             complement(7774..8475)
                     /locus_tag="PWS82_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906911.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="PWS82_001806"
     CDS             8661..9260
                     /locus_tag="PWS82_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0906912.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="PWS82_001807"
     CDS             9341..10354
                     /locus_tag="PWS82_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0906913.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="PWS82_001808"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="PWS82_001808"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL0906914.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="PWS82_001809"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="PWS82_001809"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL0906915.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="PWS82_001810"
     CDS             11802..13175
                     /locus_tag="PWS82_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0906916.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="PWS82_001811"
     CDS             13532..15676
                     /locus_tag="PWS82_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0906917.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4617 a         3133 c         2916 g         5027 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag ggg
//
LOCUS       DANJJW010000061        15587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000061 DANJJW010000000
VERSION     DANJJW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(341..707)
                     /gene="ssrA"
                     /locus_tag="PWS82_001812"
     tmRNA           complement(341..707)
                     /gene="ssrA"
                     /locus_tag="PWS82_001812"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(878..1651)
                     /locus_tag="PWS82_001813"
     CDS             complement(878..1651)
                     /locus_tag="PWS82_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0906918.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1795..2145
                     /locus_tag="PWS82_001814"
     CDS             1795..2145
                     /locus_tag="PWS82_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL0906919.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2126..2842
                     /locus_tag="PWS82_001815"
     CDS             2126..2842
                     /locus_tag="PWS82_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0906920.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2842..4290
                     /locus_tag="PWS82_001816"
     CDS             2842..4290
                     /locus_tag="PWS82_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0906921.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4360..4869)
                     /locus_tag="PWS82_001817"
     CDS             complement(4360..4869)
                     /locus_tag="PWS82_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906922.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4859..5584)
                     /locus_tag="PWS82_001818"
     CDS             complement(4859..5584)
                     /locus_tag="PWS82_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906923.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5807..7927)
                     /locus_tag="PWS82_001819"
     CDS             complement(5807..7927)
                     /locus_tag="PWS82_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0906924.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8157..9335
                     /locus_tag="PWS82_001820"
     CDS             8157..9335
                     /locus_tag="PWS82_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL0906925.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9351..10109
                     /gene="aroD"
                     /locus_tag="PWS82_001821"
     CDS             9351..10109
                     /gene="aroD"
                     /locus_tag="PWS82_001821"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL0906926.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10132..10617
                     /gene="ybaK"
                     /locus_tag="PWS82_001822"
     CDS             10132..10617
                     /gene="ybaK"
                     /locus_tag="PWS82_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL0906927.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10688..11941)
                     /locus_tag="PWS82_001823"
     CDS             complement(10688..11941)
                     /locus_tag="PWS82_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0906928.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12059..13408
                     /gene="gdhA"
                     /locus_tag="PWS82_001824"
     CDS             12059..13408
                     /gene="gdhA"
                     /locus_tag="PWS82_001824"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL0906929.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13520..14866
                     /locus_tag="PWS82_001825"
     CDS             13520..14866
                     /locus_tag="PWS82_001825"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL0906930.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15174..15560)
                     /locus_tag="PWS82_001826"
     CDS             complement(15174..15560)
                     /locus_tag="PWS82_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0906931.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5092 a         2778 c         3143 g         4574 t
ORIGIN      
        1 gagtgaacaa ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa
       61 cttatttgaa ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt
      121 tcacctcaaa aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc
      181 tgaatacgta ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca
      241 aaaaattttg tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa
      301 caacgttttt catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc
      361 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      421 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      481 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      541 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      601 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      661 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
      721 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
      781 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
      841 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
      901 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
      961 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
     1021 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
     1081 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
     1141 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
     1201 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
     1261 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1321 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1381 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1441 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1501 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1561 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1621 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1681 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     1741 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     1801 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     1861 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     1921 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     1981 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     2041 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     2101 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     2161 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     2221 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     2281 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2341 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2401 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2461 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt
     2521 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2581 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2641 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     2701 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     2761 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     2821 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga
     2881 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     2941 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     3001 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     3061 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     3121 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     3181 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     3241 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3301 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3361 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3421 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3481 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3541 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3601 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3661 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     3721 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     3781 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     3841 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     3901 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     3961 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     4021 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     4081 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     4141 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     4201 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     4261 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4321 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4381 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4441 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4501 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4561 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4621 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4681 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     4741 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     4801 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     4861 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     4921 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     4981 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     5041 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     5101 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     5161 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     5221 taaaaaggaa taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct
     5281 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5341 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5401 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5461 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5521 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5581 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5641 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     5701 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     5761 gagcagcgac aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac
     5821 ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt
     5881 gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt
     5941 ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac
     6001 tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt
     6061 tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa
     6121 tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa
     6181 caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc
     6241 ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat
     6301 caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca
     6361 tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc
     6421 caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa
     6481 ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta
     6541 cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat
     6601 tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata
     6661 cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt
     6721 ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat
     6781 aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga
     6841 atgtaccagc gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat
     6901 caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca
     6961 agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt
     7021 gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag
     7081 gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct
     7141 ggaagctttc taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt
     7201 catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac
     7261 ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag
     7321 cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg
     7381 gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga
     7441 tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca
     7501 gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc
     7561 gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg
     7621 taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa
     7681 gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa
     7741 ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc
     7801 cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta
     7861 aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac
     7921 atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt
     7981 acacagctca cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc
     8041 agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag
     8101 acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga
     8161 aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa
     8221 aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc
     8281 aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat
     8341 tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa
     8401 aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg
     8461 gagaaggaga cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt
     8521 cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa
     8581 tctacccgga aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg
     8641 agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag
     8701 taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag
     8761 aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta
     8821 gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg
     8881 tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg
     8941 atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa
     9001 gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga
     9061 aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt
     9121 tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat
     9181 tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt
     9241 tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga
     9301 aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg
     9361 taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg
     9421 tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg
     9481 aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg
     9541 ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg
     9601 aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac
     9661 acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa
     9721 gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta
     9781 aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag
     9841 ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg
     9901 agttgactag acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa
     9961 atcttggtaa aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt
    10021 ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa
    10081 ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag
    10141 aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca
    10201 gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt
    10261 ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct
    10321 cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca
    10381 gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata
    10441 cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt
    10501 gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta
    10561 gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc
    10621 atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt
    10681 ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg
    10741 ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca
    10801 gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc
    10861 cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt
    10921 tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat
    10981 ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag
    11041 cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt
    11101 gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta
    11161 attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg
    11221 tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta
    11281 aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg
    11341 atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga
    11401 tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga
    11461 taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt
    11521 tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac
    11581 gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat
    11641 gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat
    11701 tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat
    11761 catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt
    11821 ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga
    11881 tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca
    11941 ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt
    12001 tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat
    12061 gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg
    12121 gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga
    12181 cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt
    12241 tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta
    12301 ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag
    12361 tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac
    12421 cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga
    12481 taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc
    12541 atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt
    12601 cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga
    12661 tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa
    12721 acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag
    12781 cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac
    12841 atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa
    12901 agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc
    12961 tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc
    13021 atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat
    13081 caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca
    13141 tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc
    13201 tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga
    13261 gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta
    13321 cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa
    13381 agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca
    13441 ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa
    13501 cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt
    13561 tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt
    13621 acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta
    13681 tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga
    13741 agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt
    13801 cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt
    13861 ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga
    13921 tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc
    13981 gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg
    14041 tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg
    14101 ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt
    14161 tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc
    14221 tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc
    14281 agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga
    14341 accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa
    14401 agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg
    14461 acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc
    14521 tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca
    14581 aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac
    14641 agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg
    14701 ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa
    14761 cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa
    14821 accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc
    14881 gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca
    14941 tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt
    15001 gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag
    15061 gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat
    15121 cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat
    15181 aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct
    15241 atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca
    15301 ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca
    15361 gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg
    15421 atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa
    15481 gtagactgat agtagtccca acctttccat cctataaaag caatcaatat gataacgatc
    15541 aaccctaaaa tttttttcat gaaagatctc cttttttatt tttatta
//
LOCUS       DANJJW010000062        15230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000062 DANJJW010000000
VERSION     DANJJW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            278..787
                     /locus_tag="PWS82_001827"
     CDS             278..787
                     /locus_tag="PWS82_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0906932.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWS82_001828"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWS82_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0906933.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWS82_001829"
     CDS             2322..3302
                     /locus_tag="PWS82_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0906934.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWS82_001830"
     CDS             3424..4842
                     /locus_tag="PWS82_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL0906935.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWS82_001831"
     CDS             4913..6382
                     /locus_tag="PWS82_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0906936.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWS82_001832"
     CDS             6392..6859
                     /locus_tag="PWS82_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0906937.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWS82_001833"
     CDS             6895..7767
                     /locus_tag="PWS82_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0906938.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="PWS82_001834"
     CDS             7848..10382
                     /locus_tag="PWS82_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0906939.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="PWS82_001835"
     CDS             10538..11311
                     /locus_tag="PWS82_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0906940.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="PWS82_001836"
     CDS             11304..12095
                     /locus_tag="PWS82_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0906941.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="PWS82_001837"
     CDS             12105..12596
                     /locus_tag="PWS82_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0906942.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="PWS82_001838"
     CDS             12614..13024
                     /locus_tag="PWS82_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0906943.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="PWS82_001839"
     CDS             13028..14428
                     /locus_tag="PWS82_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL0906944.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="PWS82_001840"
     CDS             14451..14825
                     /locus_tag="PWS82_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0906945.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DANJJW010000063        15140 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000063 DANJJW010000000
VERSION     DANJJW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS82_001841"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS82_001841"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL0906946.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS82_001842"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS82_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL0906947.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS82_001843"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS82_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL0906948.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS82_001844"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS82_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL0906949.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS82_001845"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS82_001845"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL0906950.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS82_001846"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS82_001846"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL0906951.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PWS82_001847"
     CDS             complement(8053..8523)
                     /locus_tag="PWS82_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL0906952.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PWS82_001848"
     CDS             complement(8569..8994)
                     /locus_tag="PWS82_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL0906953.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PWS82_001849"
     CDS             complement(9061..9330)
                     /locus_tag="PWS82_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL0906954.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PWS82_001850"
     CDS             complement(9341..10936)
                     /locus_tag="PWS82_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0906955.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS82_001851"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS82_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL0906956.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PWS82_001852"
     CDS             complement(14489..15049)
                     /locus_tag="PWS82_001852"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL0906957.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3908 a         3431 c         2684 g         5117 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact
//
LOCUS       DANJJW010000064        15072 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000064 DANJJW010000000
VERSION     DANJJW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..170
                     /locus_tag="PWS82_001853"
     CDS             <1..170
                     /locus_tag="PWS82_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0906958.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="PWS82_001854"
     CDS             complement(553..1281)
                     /locus_tag="PWS82_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL0906959.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="PWS82_001855"
     CDS             complement(1259..2833)
                     /locus_tag="PWS82_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0906960.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="PWS82_001856"
     CDS             3062..4000
                     /locus_tag="PWS82_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0906961.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="PWS82_001857"
     CDS             4012..4923
                     /locus_tag="PWS82_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0906962.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="PWS82_001858"
     CDS             4947..6419
                     /locus_tag="PWS82_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0906963.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="PWS82_001859"
     CDS             6470..7081
                     /locus_tag="PWS82_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0906964.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="PWS82_001860"
     CDS             7111..9024
                     /locus_tag="PWS82_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL0906965.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="PWS82_001861"
     CDS             9152..12133
                     /locus_tag="PWS82_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL0906966.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="PWS82_001862"
     CDS             12130..13791
                     /locus_tag="PWS82_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL0906967.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="PWS82_001863"
     CDS             13831..14763
                     /locus_tag="PWS82_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL0906968.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DANJJW010000065        14525 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000065 DANJJW010000000
VERSION     DANJJW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(330..692)
                     /locus_tag="PWS82_001864"
     CDS             complement(330..692)
                     /locus_tag="PWS82_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906969.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="PWS82_001865"
     CDS             complement(766..1146)
                     /locus_tag="PWS82_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL0906970.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1210..2403
                     /locus_tag="PWS82_001866"
     CDS             1210..2403
                     /locus_tag="PWS82_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0906971.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2480..3064)
                     /locus_tag="PWS82_001867"
     CDS             complement(2480..3064)
                     /locus_tag="PWS82_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0906972.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="PWS82_001868"
     CDS             3265..4263
                     /locus_tag="PWS82_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0906973.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="PWS82_001869"
     CDS             4375..4884
                     /locus_tag="PWS82_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL0906974.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="PWS82_001870"
     CDS             complement(4914..5573)
                     /locus_tag="PWS82_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL0906975.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="PWS82_001871"
     CDS             complement(5575..6339)
                     /locus_tag="PWS82_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL0906976.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="PWS82_001872"
     CDS             complement(6347..7024)
                     /locus_tag="PWS82_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL0906977.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="PWS82_001873"
     CDS             complement(7061..8446)
                     /locus_tag="PWS82_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0906978.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="PWS82_001874"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWS82_001874"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="PWS82_001875"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="PWS82_001875"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL0906979.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="PWS82_001876"
     CDS             9385..10242
                     /locus_tag="PWS82_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906980.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="PWS82_001877"
     CDS             complement(10323..10658)
                     /locus_tag="PWS82_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0906981.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="PWS82_001878"
     CDS             complement(10725..12095)
                     /locus_tag="PWS82_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL0906982.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="PWS82_001879"
     CDS             complement(12173..12709)
                     /locus_tag="PWS82_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0906983.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="PWS82_001880"
     CDS             13018..14499
                     /locus_tag="PWS82_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0906984.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DANJJW010000066        14511 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000066 DANJJW010000000
VERSION     DANJJW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            124..1869
                     /gene="dnaX"
                     /locus_tag="PWS82_001881"
     CDS             124..1869
                     /gene="dnaX"
                     /locus_tag="PWS82_001881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL0906985.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            1889..2203
                     /locus_tag="PWS82_001882"
     CDS             1889..2203
                     /locus_tag="PWS82_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL0906986.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            2228..2824
                     /gene="recR"
                     /locus_tag="PWS82_001883"
     CDS             2228..2824
                     /gene="recR"
                     /locus_tag="PWS82_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL0906987.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            2888..3532
                     /locus_tag="PWS82_001884"
     CDS             2888..3532
                     /locus_tag="PWS82_001884"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL0906988.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            3547..3876
                     /locus_tag="PWS82_001885"
     CDS             3547..3876
                     /locus_tag="PWS82_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL0906989.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            3890..4828
                     /gene="holB"
                     /locus_tag="PWS82_001886"
     CDS             3890..4828
                     /gene="holB"
                     /locus_tag="PWS82_001886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL0906990.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            4864..5688
                     /locus_tag="PWS82_001887"
     CDS             4864..5688
                     /locus_tag="PWS82_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL0906991.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            5681..6028
                     /locus_tag="PWS82_001888"
     CDS             5681..6028
                     /locus_tag="PWS82_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL0906992.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            6097..6969
                     /gene="rsmI"
                     /locus_tag="PWS82_001889"
     CDS             6097..6969
                     /gene="rsmI"
                     /locus_tag="PWS82_001889"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL0906993.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            7078..8199
                     /gene="dinB"
                     /locus_tag="PWS82_001890"
     CDS             7078..8199
                     /gene="dinB"
                     /locus_tag="PWS82_001890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL0906994.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(8253..8855)
                     /gene="nrdG"
                     /locus_tag="PWS82_001891"
     CDS             complement(8253..8855)
                     /gene="nrdG"
                     /locus_tag="PWS82_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL0906995.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(9170..11359)
                     /gene="nrdD"
                     /locus_tag="PWS82_001892"
     CDS             complement(9170..11359)
                     /gene="nrdD"
                     /locus_tag="PWS82_001892"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL0906996.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            11658..12452
                     /locus_tag="PWS82_001893"
     CDS             11658..12452
                     /locus_tag="PWS82_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0906997.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            12442..14484
                     /locus_tag="PWS82_001894"
     CDS             12442..14484
                     /locus_tag="PWS82_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0906998.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         4687 a         2551 c         3075 g         4198 t
ORIGIN      
        1 ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag aaggatagta
       61 gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa aagggggatc
      121 cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt
      181 ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa aacctcacat
      241 gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa gattttcgca
      301 aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt
      361 cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac
      421 ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg
      481 tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt caatgccttg
      541 ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac gaccgaacct
      601 cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa acggatcaat
      661 acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat cagctatgaa
      721 ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga tgcattgagt
      781 attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga tgccatgcaa
      841 gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa
      901 gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa agaatcacgt
      961 cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca gcaagcacct
     1021 aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt ggcacaaact
     1081 gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca aaatgaaatt
     1141 cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact tgcacagcta
     1201 ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat agtaaaacag
     1261 ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag taatacagga
     1321 attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca gacaagtacc
     1381 tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag acagcatctg
     1441 aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac tcagcgagca
     1501 atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat tgcgtttgat
     1561 tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc ggtacacaac
     1621 aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac aagagaaagc
     1681 tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac agaaaactct
     1741 tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca agaagtacca
     1801 gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt tacagtggta
     1861 gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata tgcaaggcat
     1921 gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa
     1981 aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta caggcgatcg
     2041 tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata tcgagatgct
     2101 tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg tggaaactga
     2161 gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa ctgaaggagt
     2221 aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca tgaaacttcc
     2281 aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata tgaaagatga
     2341 agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag
     2401 tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga
     2461 tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg
     2521 agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg aagggacagg
     2581 tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag atgtaaaaga
     2641 agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt atctctcacg
     2701 tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt ctgtaggctc
     2761 tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa gacgtgagtt
     2821 ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa gagatggggg
     2881 caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag gaaaaaccag
     2941 catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa tcattcaaac
     3001 aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc ttgatccaaa
     3061 aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa gaagacaaca
     3121 tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat gcgatagatt
     3181 tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta ccgagattgc
     3241 tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt atcttgacgt
     3301 agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt
     3361 agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa agctggcaga
     3421 agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag aagtgctgca
     3481 aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact aggaaggtga
     3541 acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg tttatcaaat
     3601 gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg cttccttaag
     3661 gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga agcattggaa
     3721 atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt tacactggat
     3781 atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg tgccacagtc
     3841 ttcgtcttac ctgttgaagg attccatcaa ttttaggagg agctgagcta tgaatcaaga
     3901 aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga gttttgagca
     3961 tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa aacatgaagt
     4021 aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc tgccttgtgg
     4081 aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt tggttattga
     4141 accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg aattcagtcg
     4201 cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag
     4261 cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt ttttagcggt
     4321 gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt gtcaagttct
     4381 gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag agcagattcc
     4441 attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag cagttgaaat
     4501 ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt tcgtttactt
     4561 gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa aatcatttaa
     4621 agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc aggaagcaca
     4681 gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc agataattga
     4741 acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg ctgttgcgga
     4801 acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc aaggaggatg
     4861 gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta ttattttgca
     4921 ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca gcaatcctta
     4981 catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct tcctgaagct
     5041 atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa
     5101 aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa
     5161 atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt ttctttcaca
     5221 gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt atttcgtact
     5281 cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg cggaattggt
     5341 ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc
     5401 aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg tttatgcgga
     5461 cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa aaaagaactc
     5521 cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt agggttgaat
     5581 ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt cgaatatgaa
     5641 tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga gatcattata
     5701 cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac ttgttgaaat
     5761 gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg
     5821 tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg aaaatgtgaa
     5881 gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag gattccatgt
     5941 ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt tttgtctaga
     6001 agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga gcggagaaat
     6061 actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa aagtttcaag
     6121 ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa tttagaagat
     6181 atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg
     6241 cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa aagtttacat
     6301 gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg
     6361 attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca tgagctagtt
     6421 ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac agctgggatg
     6481 acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg cttccttcca
     6541 cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac agttacgctg
     6601 attttttatg agtcacctta tcggatcact gctacattga cgaatttttt agaaattttt
     6661 ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga
     6721 ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt
     6781 ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga agagttggga
     6841 acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc
     6901 aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa
     6961 ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat cagctactaa
     7021 cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga ggtgaggatg
     7081 atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca tattgatatg
     7141 gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg acatccatta
     7201 gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac agcaaattat
     7261 gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta tgagctttgt
     7321 ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc caaacaagta
     7381 cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga tgaagcgtat
     7441 cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc
     7501 caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag ttacaataag
     7561 tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt agttgcccct
     7621 caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg ggtagggaag
     7681 aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct atatgaatgc
     7741 acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta tcgtaaagtc
     7801 agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt gggaaaagag
     7861 catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta
     7921 gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac agtagttttg
     7981 aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc agaatatatc
     8041 tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat cttgggagta
     8101 gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat gacttatgaa
     8161 aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg agagttgaaa
     8221 ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt tttgatttgt
     8281 tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt ggattctaaa
     8341 gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag ttttaggtca
     8401 cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag gagttctaat
     8461 ttatcttctg aatcttgtag caattcatca aaggcatagc ctgtccatga ccagatatct
     8521 tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac atctgtattc
     8581 aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg caagtcagcg
     8641 atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct
     8701 ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact gttgcggaca
     8761 ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg gctgtagtct
     8821 tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc atttaaggtt
     8881 caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa caaactgacc
     8941 taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca aagatacaaa
     9001 gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg aataaacggt
     9061 gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg aggaactatt
     9121 tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct catttcatgt
     9181 gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga gcttgcggat
     9241 ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca tgatttccac
     9301 attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa ccacattgat
     9361 agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc
     9421 atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag tagtggatga
     9481 atccacctga acaatatttt ggataatcct tctcaaattc caatttttca aatggcgttg
     9541 ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct gtgatatttt
     9601 cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga ctttcacttg
     9661 gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct gcattagctt
     9721 ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc
     9781 caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat acagtagctc
     9841 gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc ccgaaagaac
     9901 catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt tctacacgat
     9961 agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag aatttattct
    10021 gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca cctaaattca
    10081 ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag aatgaacgac
    10141 agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa ttcagaatat
    10201 caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg taattaggat
    10261 cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg aagatagctg
    10321 tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt tgaatttctc
    10381 tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg
    10441 aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag atatcttttt
    10501 tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat tcttccgcgt
    10561 cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca tctgttcggt
    10621 cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt gagatttgtg
    10681 ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt ttgaacccat
    10741 tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta taaggatgat
    10801 aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata tgtggtggca
    10861 acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc tgtgtattga
    10921 aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg ttgattagct
    10981 tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt ttggtacgat
    11041 aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata tgttccacga
    11101 tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt tcttggacaa
    11161 ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag ccgtgaattt
    11221 tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca acatggcggc
    11281 catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg acagtttttt
    11341 cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc tatcataaaa
    11401 caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg tgctgtataa
    11461 ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga aaacttgtag
    11521 ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga ataaaatttt
    11581 ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta gatatgctac
    11641 tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa aagatgatcg
    11701 aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa gccttagata
    11761 atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca agtggtgcag
    11821 gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg ggcagtgtct
    11881 cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa
    11941 aaaaactagg attcattttt caagagttca atttgatcga taccctaaat gcaaaagaca
    12001 atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa aagagagtaa
    12061 gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc gatgaacttt
    12121 ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag ccacaagtga
    12181 tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa ttgttgaact
    12241 acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc catgatccat
    12301 atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt ttttctgaag
    12361 ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg caggcgacga
    12421 ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt ttttcggcag
    12481 tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat ctattattcc
    12541 ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga tacttcgata
    12601 gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt tttcgtattc
    12661 agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta tcagcttttt
    12721 ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat aattggattg
    12781 ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt ttcaatgatt
    12841 ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat tccatcagta
    12901 gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata ttcaatatgg
    12961 aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc agatagttca
    13021 acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact acttttaacg
    13081 agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat cagttatcat
    13141 tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg tgtggtcggt
    13201 acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag taaatggaag
    13261 atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca tttgctcaaa
    13321 agttggcgta caaattctct gattacagtc attctcggaa tttctttaat gacaattggc
    13381 ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc cccgatggac
    13441 tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga ggaagggcag
    13501 aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta tagtcaaaat
    13561 ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata tcaagccttc
    13621 agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca tactgttttg
    13681 cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag tattggactg
    13741 ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg tgacgaaaca
    13801 atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa agcaaaaggt
    13861 gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa
    13921 cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc ttatgatatc
    13981 gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga ccaaatgaag
    14041 gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta ttcgaaattg
    14101 cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt cgggatgatc
    14161 gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc agaggaagaa
    14221 aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat cagacggatg
    14281 atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat cactcacgca
    14341 gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg gttggcttat
    14401 cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac gagtcagtta
    14461 tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa t
//
LOCUS       DANJJW010000067        14344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000067 DANJJW010000000
VERSION     DANJJW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            110..491
                     /locus_tag="PWS82_001895"
                     /pseudo
     CDS             110..491
                     /locus_tag="PWS82_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="PWS82_001896"
     CDS             622..1572
                     /locus_tag="PWS82_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0906999.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="PWS82_001897"
     CDS             2090..2545
                     /locus_tag="PWS82_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0907000.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="PWS82_001898"
     CDS             2886..3488
                     /locus_tag="PWS82_001898"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907001.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="PWS82_001899"
     CDS             3582..3914
                     /locus_tag="PWS82_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HDL0907002.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4065..5044)
                     /locus_tag="PWS82_001900"
                     /pseudo
     CDS             complement(4065..5044)
                     /locus_tag="PWS82_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5049..5816)
                     /locus_tag="PWS82_001901"
     CDS             complement(5049..5816)
                     /locus_tag="PWS82_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0907003.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="PWS82_001902"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="PWS82_001902"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL0907004.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="PWS82_001903"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="PWS82_001903"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL0907005.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="PWS82_001904"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="PWS82_001904"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL0907006.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="PWS82_001905"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="PWS82_001905"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0907007.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="PWS82_001906"
     CDS             9217..9534
                     /locus_tag="PWS82_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0907008.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="PWS82_001907"
     CDS             9534..11240
                     /locus_tag="PWS82_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0907009.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="PWS82_001908"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="PWS82_001908"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0907010.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="PWS82_001909"
     CDS             12811..13083
                     /locus_tag="PWS82_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDL0907011.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="PWS82_001910"
     CDS             13104..14003
                     /locus_tag="PWS82_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL0907012.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DANJJW010000068        13810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000068 DANJJW010000000
VERSION     DANJJW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(221..1423)
                     /locus_tag="PWS82_001911"
     CDS             complement(221..1423)
                     /locus_tag="PWS82_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0907013.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="PWS82_001912"
     CDS             complement(1426..1620)
                     /locus_tag="PWS82_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0907014.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="PWS82_001913"
     CDS             complement(1667..2515)
                     /locus_tag="PWS82_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL0907015.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="PWS82_001914"
     CDS             complement(2720..3070)
                     /locus_tag="PWS82_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907016.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="PWS82_001915"
     CDS             3665..4111
                     /locus_tag="PWS82_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL0907017.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="PWS82_001916"
     CDS             4735..5727
                     /locus_tag="PWS82_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0907018.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="PWS82_001917"
     CDS             5743..6627
                     /locus_tag="PWS82_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0907019.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="PWS82_001918"
     CDS             6676..8283
                     /locus_tag="PWS82_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0907020.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="PWS82_001919"
     CDS             8284..9768
                     /locus_tag="PWS82_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL0907021.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="PWS82_001920"
     CDS             9945..10937
                     /locus_tag="PWS82_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0907022.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="PWS82_001921"
     CDS             10989..11858
                     /locus_tag="PWS82_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0907023.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWS82_001922"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWS82_001922"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0907024.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DANJJW010000069        13731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000069 DANJJW010000000
VERSION     DANJJW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            1..1452
                     /locus_tag="PWS82_001923"
     CDS             1..1452
                     /locus_tag="PWS82_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0907025.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="PWS82_001924"
     CDS             1801..2433
                     /locus_tag="PWS82_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL0907026.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="PWS82_001925"
     CDS             3021..4460
                     /locus_tag="PWS82_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0907027.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="PWS82_001926"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="PWS82_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="PWS82_001927"
     CDS             5222..5437
                     /locus_tag="PWS82_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907028.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="PWS82_001928"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="PWS82_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="PWS82_001929"
     CDS             complement(6097..7008)
                     /locus_tag="PWS82_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907029.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="PWS82_001930"
     CDS             complement(7027..8031)
                     /locus_tag="PWS82_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0907030.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="PWS82_001931"
     CDS             complement(8028..10151)
                     /locus_tag="PWS82_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0907031.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="PWS82_001932"
     CDS             complement(10156..12603)
                     /locus_tag="PWS82_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0907032.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="PWS82_001933"
     CDS             complement(12590..12979)
                     /locus_tag="PWS82_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907033.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="PWS82_001934"
     CDS             complement(13039..13542)
                     /locus_tag="PWS82_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0907034.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="PWS82_001935"
     CDS             complement(13555..13716)
                     /locus_tag="PWS82_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907035.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DANJJW010000070        13721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000070 DANJJW010000000
VERSION     DANJJW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            63..257
                     /locus_tag="PWS82_001936"
     CDS             63..257
                     /locus_tag="PWS82_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907036.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            526..825
                     /locus_tag="PWS82_001937"
     CDS             526..825
                     /locus_tag="PWS82_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907037.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(922..1356)
                     /locus_tag="PWS82_001938"
     CDS             complement(922..1356)
                     /locus_tag="PWS82_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907038.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1588..2298)
                     /locus_tag="PWS82_001939"
     CDS             complement(1588..2298)
                     /locus_tag="PWS82_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0907039.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2288..3148)
                     /locus_tag="PWS82_001940"
     CDS             complement(2288..3148)
                     /locus_tag="PWS82_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL0907040.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3153..3791)
                     /locus_tag="PWS82_001941"
     CDS             complement(3153..3791)
                     /locus_tag="PWS82_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL0907041.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3806..4105)
                     /locus_tag="PWS82_001942"
     CDS             complement(3806..4105)
                     /locus_tag="PWS82_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907042.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4148..5632)
                     /locus_tag="PWS82_001943"
     CDS             complement(4148..5632)
                     /locus_tag="PWS82_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0907043.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5652..6113)
                     /locus_tag="PWS82_001944"
     CDS             complement(5652..6113)
                     /locus_tag="PWS82_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0907044.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6131..7198)
                     /gene="ulaG"
                     /locus_tag="PWS82_001945"
     CDS             complement(6131..7198)
                     /gene="ulaG"
                     /locus_tag="PWS82_001945"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL0907045.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7416..8186
                     /locus_tag="PWS82_001946"
     CDS             7416..8186
                     /locus_tag="PWS82_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0907046.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8459..9286)
                     /locus_tag="PWS82_001947"
     CDS             complement(8459..9286)
                     /locus_tag="PWS82_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0907047.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9854..11089
                     /locus_tag="PWS82_001948"
     CDS             9854..11089
                     /locus_tag="PWS82_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0907048.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12088..12348)
                     /locus_tag="PWS82_001949"
     CDS             complement(12088..12348)
                     /locus_tag="PWS82_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0907049.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12375..13676)
                     /locus_tag="PWS82_001950"
     CDS             complement(12375..13676)
                     /locus_tag="PWS82_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907050.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4404 a         2818 c         2167 g         4332 t
ORIGIN      
        1 aatcattttc acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc
       61 aaatgtatta tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta
      121 tcagtaacga atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat
      181 gtgccataaa aataaagaac gagctagaaa aagaactgac ttgtggtaaa gctgttgtag
      241 atatcatcga aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta
      301 caaattgcta agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa
      361 taaatgtttg tataccatat gattatttca atatgtatcc tggaattgga gatctcgtag
      421 agctatatca agatgaatca aatatacttg tcattcatca tgaaatatag agacttataa
      481 aattgtttat ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt
      541 gaatttgaca gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa
      601 gcaatttccc acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg
      661 cgtactcgtt cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat
      721 cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg
      781 caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga
      841 tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt
      901 tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt
      961 atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata
     1021 aaattttttt gttttctcag tatcatctgt caataattcg atttggactg gtccataact
     1081 agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct
     1141 cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac
     1201 gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc
     1261 tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc
     1321 agaaatctca tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat
     1381 tctccctcta ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa
     1441 agattctctc gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat
     1501 aaaaaggttg tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt
     1561 taattttcta cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt
     1621 gcgaaaataa tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag
     1681 ctcgttttct ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt
     1741 cataggtgaa tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc
     1801 gtttggatct aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc
     1861 gtaagcttct cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa
     1921 agcatcagca tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac
     1981 ggagtgcgtg gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac
     2041 aacatgtgtg gggagatctg aagatggttt taagctatct tcttctatta tatttcccga
     2101 caaatctgtt accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt
     2161 aatgacgatg actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac
     2221 taatccgtat cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat
     2281 tttttcttca caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg
     2341 cctgctggat ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg
     2401 ttccatcata atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc
     2461 taccaaaagg aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt
     2521 ttaagtggat agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt
     2581 ccggccatgc ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct
     2641 gttctttgat ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca
     2701 cggaaagaat gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa
     2761 agtcttctct ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga
     2821 ttactttgac gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg
     2881 cttttttctt ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa
     2941 tcgaatggat acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt
     3001 cagaccaatc taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat
     3061 cgaagtccat ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg
     3121 ctttttcata aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct
     3181 ttgaagtttt gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata
     3241 aaggtataca catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt
     3301 acagaaacat taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc
     3361 caagtttcac cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg
     3421 atgttatgcc aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat
     3481 tcacctattt ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc
     3541 atccagtctg ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat
     3601 ttcgtatcag cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt
     3661 tgtccttcct gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact
     3721 ttcataacat ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc
     3781 ggcctgctca tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa
     3841 atttttcact gatttctttg actgacatca catttttgat accgataact gtaactcctt
     3901 ttttctctgc ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt
     3961 ggccagctga actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt
     4021 ttagttcttt cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat
     4081 ttgcacacga tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga
     4141 ttttctttta tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat
     4201 aaccatcagg atttttccga tattgtaact gcggaatagc caataaacca atgacaacta
     4261 cggcaattcc agcataacct aggactttca tgattactgt aaacaatggc caagctgttg
     4321 cccagtcgaa catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata
     4381 atgctgaacc gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga
     4441 atccacctcg gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga
     4501 aaccggctat gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact
     4561 gtcccaaagc accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag
     4621 tagcagctac gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga
     4681 aagattgagt tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata
     4741 ccgcaaaatt taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga
     4801 attgcgcttg aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata
     4861 gcatgagtaa cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt
     4921 tgatatcttc aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt
     4981 tcatccactc agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg
     5041 caaatccggc accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg
     5101 tccaataaca acctaaaatc agtcccataa aaatcaatgc ttgtatccta cctagatcag
     5161 gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg
     5221 tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa
     5281 caaatgcaat cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta
     5341 atccagcatc cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga
     5401 accgttcctt tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa
     5461 cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg
     5521 ggcgtttcag caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg
     5581 tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc
     5641 tcctctatca attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta
     5701 gagatgagac gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt
     5761 tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc
     5821 tcattctccc cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct
     5881 gttccaaaaa caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc
     5941 acaatataag gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca
     6001 ataatttcct tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg
     6061 atttctttat ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc
     6121 cccttctttc ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt
     6181 caaatcctcg ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc
     6241 cgcctacttg ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc
     6301 aaatttcagt gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag
     6361 gaatcactac ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt
     6421 tgtctgttat ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt
     6481 cattaccatg tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc
     6541 ctcctgatgt ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc
     6601 cttttaatac ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta
     6661 aagctactac attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt
     6721 tttcttcagg aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg
     6781 ttgcttcagg acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat
     6841 ggatatgtgt aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag
     6901 gctgagtacg taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc
     6961 cttttttcat catgccattc ccatgactac gttttcccgt cccacaccat agatcacata
     7021 aaatattcgt tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag
     7081 caaaagttcc aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact
     7141 caggaaatgt ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt
     7201 gcttgctccc cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc
     7261 catgaaaacc cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg
     7321 attgtttcag tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat
     7381 ccatggatca agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg
     7441 aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg
     7501 aattagctga atcccttgat gtatctatta gtacgatacg aagagattta aatttattag
     7561 aagaaaaaaa cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga
     7621 ctgattttga tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta
     7681 aatacattaa cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac
     7741 tggattttct tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact
     7801 ctccgcacaa agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag
     7861 aagcactagt gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta
     7921 tcattggctg tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat
     7981 caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc
     8041 atcgaaaaat cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt
     8101 tgatcactga taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa
     8161 aaaccattca agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca
     8221 aaaaaatgaa taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag
     8281 gaatcccaag atgtatccat ccaatcgata ccatcataat ctattgcttc ttttataacc
     8341 gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc
     8401 acacctcctt ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt
     8461 atttcaggtc gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt
     8521 tttttgcgtc catatatctt tctaaataat catcttctcc taattgctta tggcaagaag
     8581 caatttcata aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa
     8641 tacccttctc acataattgg attgctttat cataatcctc gattttacgg taataggtgg
     8701 cgatatcata atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt
     8761 taagtgtacg ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca
     8821 ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca
     8881 cttgattaaa ataaaataat gctgcatctg gttttttcgc ataaagatga tcaagcagtc
     8941 cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg
     9001 tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat
     9061 ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat
     9121 tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga
     9181 tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct
     9241 ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca atttccttcc
     9301 ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa aagttctaaa caaccaaaaa
     9361 tgtttagaac agaataaaaa ttttaatttt aaaataatat tcaaattaaa caactggcgt
     9421 atctgctcct tgatttcttt ttgtgttatc tgaataatta tcgtttacag ttataacaca
     9481 tagtgaaaat tgtcatggcg agaaagtcta agaatatttt taaacataac tatttctttc
     9541 ttacgtgtgt ataatgacga cagcacacct atcaattaat ataattattt tattttttta
     9601 tgttctcatg tttattttta atgacacaac ttctcgtatt tatctggcac aacatataaa
     9661 tcgatcaaaa aattgttgcg tgtttttttg aaaattggag tttcaacttg attacaaaat
     9721 ttaaatttag aagttgtgaa aacagcgaga gacagtttaa tattcaaaat atacataggc
     9781 tactcgaacg ctgtgttttt tagtatacta gtaagaaacg aagggataga ctcattccaa
     9841 taaggaggat ttcatgaaga caattctttt tgctcctgca gtattcaatt tagctgagac
     9901 aacacgaatg attgaggttg ccaaagctat gaaagaccgt tattactgtg tattctttgg
     9961 cttttctaaa acttttgctc ctttgatcac agaaagtggc tttgagtatc atttactagc
    10021 gccgatcctt acgaaaaaac aagaagaaca aatcataaaa tttgaccagt tgcgtacact
    10081 taaaaaccct tttaccaaag aaatggttgc ctctcgtatc actagtgaac agagtctgat
    10141 tgctaagttt gagcctcgac tgattgtgac aggttcgaat gtgacaattt ttttatcggc
    10201 ccgcaataaa aagattcctt tagtatatgt taaaccctat gctttgagtg aacctttttt
    10261 tcaatcagag catgcgctga tgccccacga gttacgtttc ttcggccctt taagcaatcc
    10321 cctttggcac tggacaaaaa agcgtatagt aaatacccga tggatcccca atgccttcaa
    10381 acacgctgca caaaaagaaa tgcttccttt accaaaaaca agtttacaaa tgatggatgc
    10441 tgatctaaat ctcatcacaa cgcctaaact atttttggaa gaagaacagc tgccggaaaa
    10501 ttatcgaatc gttggtccaa tttttgctaa gctcagtact tccttgcctg aagaagtgct
    10561 aacttttata cagcgaaagc aacaagaaag aaaacgtatt atctatttcg caatgggtag
    10621 ttcaggcaat ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac aaccggatct
    10681 agcggttatt gcacctattg actatctgct tgatgacatt caatctgact cagaagatct
    10741 tttaataaca gaatatctgt ctagtcatct tttgcatgaa ttcattgatt tttcttttat
    10801 ccacggcggg gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt
    10861 ggggatgcaa tttgagcaaa gctgtaatat tgactattgt gtaaaatacg gaaacgcaat
    10921 cgccctcaca aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc
    10981 acccaagatt cgtcagcagg cacgcacgct gcaagccgtt tttccaataa atggtccaca
    11041 acaagcaatt caagaaatcg aaaccttcct gagcaaccac ccaatatagt ttcaagtaga
    11101 aaaagctttt ttaatagaga ctcatcagta atttcgctat tcgagaccca gacagtgaaa
    11161 atacaaaaaa agtaaatagt ggattgaaaa gttagagtta cttaaattac ggtgtctaaa
    11221 aaacacaaaa tcatccttaa aataaaagat ctgacgctat ccaaatgagc ataggtagcg
    11281 tctgccattt attcttagtc aaattctttg acacctttct ctggttggat catctcaacg
    11341 tttcttcaaa agaaagacca taataattag gcaaatacat tttcttaaaa tgtgtcatat
    11401 agatctagaa gcatcgcttt gtagagattt gtgccgctag tcccctcaac aattactaaa
    11461 tcaaatgtag gatttaccac tttatctttg ataatcaacc tccctcttct gttgagacaa
    11521 tgatctcgca atccgatgca tactcctata taaactgcca gcaatcccca catattgagt
    11581 agatcatgtc agaatgttct atgtcgtcag caacgataca ctaaaagtca cgttcaccat
    11641 gactgattgc tattcccagc acaattattt ctatacacgt ttcatgattt gaataaacac
    11701 tcacatcata catattacca ttcatacacc atactgcagc ccctactata tggttgtaag
    11761 ctcccaaatc tatatttgac ttgatcactt tggtagtaga tctactaatt ctaaatccat
    11821 tgctgataaa atttccattt tttcctcctt ctttctgcca aaacagttaa aacaataata
    11881 atctttatgt aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat tccgaataaa
    11941 cggtggtcaa aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca
    12001 tttcacaaaa atttaagaag caattttcgt aaatttcttc ttatttcttg aagctaacca
    12061 cttctgttac aaccactttt agaacattta aagcttcgat gtgacaaacc atctatgctg
    12121 ctcgatcata aaattgatag caaaaattcc acaaagtaca tatgtccccc atgaaaacaa
    12181 aacattcata tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg atgccagtgc
    12241 ccaaatccat cgattgattt gaatatgata tgtagctagc atattcaata taaggtgact
    12301 agccgttaat aaaaagataa aaacaaaaaa taaaatcagc caatacattg tttcccacct
    12361 tctaaaaacc aaacttagac agtctggact tcgttttctc cacgactttg ttcatctgac
    12421 agcatctttt tatcataagc ttttacaaat ggataataga taataaatgc tatcaatgtg
    12481 ttgatcatga tcaagataac aacacgccag tcatttcctg ctgaaagata accgccgatt
    12541 ggagctggta atgtccatgt tggcaaaaca gtaaatctat tgaccatttc taatttgact
    12601 gcaaaataac tgataatagt caaaactaaa ggaactaagt tgaacgggat gaacatgtaa
    12661 ggattcaaca tcataggagc tccaaaaatt atgggctcat tgatattgaa gataccagga
    12721 ataatagaaa aacgaccaac ctctttcaag aattttgatt tacagaagaa tataaacaaa
    12781 acagatacca cgatcgttgc tcctgcacca ccaattgtta ccgtccattg ataaaactgt
    12841 tcaggagcga tatagggtaa ttgggtggca gctgtaccat ctgccaatgc ctttgcattc
    12901 tcatccaaca tgatcagcca cattggacga acaacagccc ctacaatact catgccatgg
    12961 ataccaaatg accacaacaa atggatcaaa aacattggta gtaaaacacc aagcaaatta
    13021 ttccctaaaa tattggttaa aggctcgaat aacgataata aggcagcgtt tacatcaaag
    13081 cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc
    13141 tcaaaactac gggcaacagc aggtggcact tgttctggca ttttgatagt aatatttcga
    13201 tttttaaaga aacgatatac ttccactgca aaaatcatag ataagatccc cgcaaacatc
    13261 ccagatgctc ccaaagaact cataggtaag acccagccaa gaggtgcctc ttcaggagtt
    13321 aaaacgttta caggaacttg taacataaag aacgttccta aagataatat accacctgtc
    13381 acactatcta atccatatga tttagctaga tccgaaccca taacaaaagt tgcataaata
    13441 gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata aggggcaata
    13501 aattcattcc atccaggaaa cggtaaattg ataaagacca taaaaaatgt cccaatcaaa
    13561 atcaatgaca atgttgaaac aaccccatta cgaattgcta ataaatgacg ctgtgtacca
    13621 atttttgcca ttggtgggac aagtttctct tcgacccatt tcatcagctt ttccactaaa
    13681 accactcctt ttcaataaaa tatgaaccat tcattgcatg a
//
LOCUS       DANJJW010000071        13395 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000071 DANJJW010000000
VERSION     DANJJW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(14..706)
                     /locus_tag="PWS82_001951"
     CDS             complement(14..706)
                     /locus_tag="PWS82_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0907051.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="PWS82_001952"
     CDS             complement(1143..1712)
                     /locus_tag="PWS82_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0907052.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="PWS82_001953"
     CDS             complement(1786..2109)
                     /locus_tag="PWS82_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907053.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="PWS82_001954"
     CDS             complement(2263..3075)
                     /locus_tag="PWS82_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907054.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="PWS82_001955"
     CDS             3429..4277
                     /locus_tag="PWS82_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL0907055.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="PWS82_001956"
     CDS             4422..5363
                     /locus_tag="PWS82_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL0907056.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="PWS82_001957"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="PWS82_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="PWS82_001958"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="PWS82_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL0907057.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="PWS82_001959"
     CDS             complement(9827..12013)
                     /locus_tag="PWS82_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL0907058.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="PWS82_001960"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="PWS82_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL0907059.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="PWS82_001961"
     CDS             complement(12235..12675)
                     /locus_tag="PWS82_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL0907060.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="PWS82_001962"
     CDS             complement(12749..13288)
                     /locus_tag="PWS82_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0907061.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2380 g         4445 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DANJJW010000072        13180 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000072 DANJJW010000000
VERSION     DANJJW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            69..1808
                     /gene="yfcC"
                     /locus_tag="PWS82_001963"
     CDS             69..1808
                     /gene="yfcC"
                     /locus_tag="PWS82_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0907062.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1965..3149
                     /gene="iadA"
                     /locus_tag="PWS82_001964"
     CDS             1965..3149
                     /gene="iadA"
                     /locus_tag="PWS82_001964"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL0907063.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3260..3463
                     /locus_tag="PWS82_001965"
     CDS             3260..3463
                     /locus_tag="PWS82_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0907064.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3564..4226)
                     /locus_tag="PWS82_001966"
     CDS             complement(3564..4226)
                     /locus_tag="PWS82_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907065.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4239..5741)
                     /locus_tag="PWS82_001967"
     CDS             complement(4239..5741)
                     /locus_tag="PWS82_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907066.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6289..7968
                     /locus_tag="PWS82_001968"
     CDS             6289..7968
                     /locus_tag="PWS82_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0907067.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8095..8574
                     /locus_tag="PWS82_001969"
     CDS             8095..8574
                     /locus_tag="PWS82_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL0907068.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8793..9473
                     /locus_tag="PWS82_001970"
     CDS             8793..9473
                     /locus_tag="PWS82_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0907069.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9481..10812
                     /locus_tag="PWS82_001971"
     CDS             9481..10812
                     /locus_tag="PWS82_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0907070.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10827..11789
                     /locus_tag="PWS82_001972"
     CDS             10827..11789
                     /locus_tag="PWS82_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL0907071.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11900..12238
                     /locus_tag="PWS82_001973"
     CDS             11900..12238
                     /locus_tag="PWS82_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL0907072.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12317..13024
                     /locus_tag="PWS82_001974"
     CDS             12317..13024
                     /locus_tag="PWS82_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL0907073.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4184 a         2331 c         2897 g         3768 t
ORIGIN      
        1 gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa agaaaggaag
       61 aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc actaaattat
      121 cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg atactgactt
      181 ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat gcaaatggcg
      241 aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac gagctggata
      301 agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac
      361 tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa aatcttacgc
      421 aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag tacggcgaaa
      481 atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga acagatgatt
      541 ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga gataagtatg
      601 gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt ggtattatta
      661 tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact aagggactgg
      721 agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct acattcggga
      781 tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt gcgttaggat
      841 atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga aatgcgacct
      901 cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt
      961 ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc gctggttata
     1021 tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact tacaaatcag
     1081 atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa ttcatgcttg
     1141 ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt tggggcgtga
     1201 ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg ggattagttg
     1261 ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac atcgcttctt
     1321 ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt atggccaaag
     1381 ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg aatacgattt
     1441 tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct tgggcgatgt
     1501 acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa gcagctttga
     1561 ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa gtagcagttc
     1621 ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca gccagcttga
     1681 ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc caaatcaaga
     1741 tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt gcaataggtc
     1801 tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat aaggactcca
     1861 agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg gattattttt
     1921 taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa atcattcgac
     1981 aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt gccggtggga
     2041 agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa gtagaagaac
     2101 tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc catattttag
     2161 gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg agccaattga
     2221 ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa ggtagagata
     2281 tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc acttatgtat
     2341 acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc aaagactttt
     2401 tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac cgttcttctc
     2461 agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat
     2521 ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag ctagagctat
     2581 tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct actcatgcaa
     2641 atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt ggcacgattg
     2701 acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa gttcgcttca
     2761 gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc acgatgacct
     2821 cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta gggttaggag
     2881 taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc
     2941 ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg aaattggata
     3001 aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat aaagaaacgc
     3061 ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag
     3121 tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt tttagaaaac
     3181 gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat caataaacaa
     3241 actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata tgaaagataa
     3301 agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga gcaaagaaac
     3361 agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa atttaggcga
     3421 cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa
     3481 ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg ttcggatttt
     3541 ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt
     3601 tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag
     3661 cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca
     3721 tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga
     3781 acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat
     3841 atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc
     3901 ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc
     3961 aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt
     4021 ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa
     4081 ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt
     4141 ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa
     4201 aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt
     4261 gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat
     4321 gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact
     4381 tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct
     4441 tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg
     4501 gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta
     4561 agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga
     4621 atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc
     4681 cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagc cactagtaaa
     4741 acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg
     4801 gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga
     4861 aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg
     4921 tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga
     4981 ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct
     5041 ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct
     5101 ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct
     5161 ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta
     5221 ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga
     5281 ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca
     5341 cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt
     5401 tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa
     5461 actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa
     5521 gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct
     5581 ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca
     5641 gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat
     5701 tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt
     5761 agatgcattt gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca
     5821 caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg
     5881 gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa
     5941 gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata
     6001 ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact tatataagta
     6061 ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa
     6121 tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag
     6181 ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa
     6241 gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt
     6301 aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg
     6361 ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg
     6421 atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt
     6481 gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa
     6541 atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt
     6601 cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata
     6661 gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg
     6721 gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac
     6781 caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa
     6841 ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct
     6901 gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc
     6961 gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa
     7021 gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg
     7081 cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa
     7141 aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag
     7201 tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg
     7261 gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat
     7321 atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc
     7381 catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg
     7441 ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg
     7501 atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag
     7561 cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata
     7621 ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc
     7681 gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact
     7741 tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg
     7801 attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag
     7861 caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg
     7921 atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta
     7981 agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt
     8041 tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg
     8101 agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat
     8161 tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca
     8221 gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg
     8281 attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa
     8341 gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac
     8401 acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta
     8461 aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa
     8521 gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg
     8581 agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg
     8641 gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa
     8701 aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa
     8761 aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct
     8821 gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa
     8881 atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg
     8941 gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta
     9001 tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga
     9061 atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata
     9121 gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta
     9181 ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag
     9241 gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc gataactata
     9301 cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc
     9361 caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac
     9421 tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa
     9481 atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga
     9541 atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca
     9601 atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt
     9661 atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc
     9721 attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc
     9781 atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc
     9841 gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc
     9901 acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg
     9961 atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc
    10021 gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag
    10081 actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg
    10141 cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca
    10201 aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca
    10261 atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg
    10321 ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa
    10381 gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg
    10441 gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa
    10501 aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct
    10561 tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa
    10621 aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa
    10681 agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat
    10741 atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg
    10801 gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa
    10861 aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat
    10921 cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag
    10981 taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct
    11041 tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc
    11101 aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt
    11161 atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat
    11221 ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg
    11281 caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag
    11341 agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg
    11401 aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg
    11461 atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg
    11521 atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt
    11581 gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc
    11641 cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg
    11701 cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc
    11761 aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta
    11821 aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag
    11881 attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac
    11941 atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga
    12001 acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt
    12061 agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat
    12121 taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg
    12181 caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag
    12241 aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa
    12301 aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct
    12361 tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt
    12421 ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata
    12481 tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac
    12541 agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac
    12601 ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc
    12661 gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat
    12721 gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa
    12781 tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga
    12841 atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga
    12901 agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc
    12961 gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga
    13021 ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg
    13081 attgaatacc atttatttgg aataagggct ctttgtcaat aaggactgat gggttgtaca
    13141 aaatcaaatc tgggtcagaa tgaacctcat tctggctcag
//
LOCUS       DANJJW010000073        13156 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000073 DANJJW010000000
VERSION     DANJJW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            413..619
                     /locus_tag="PWS82_001975"
     CDS             413..619
                     /locus_tag="PWS82_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL0907074.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="PWS82_001976"
     CDS             complement(821..1822)
                     /locus_tag="PWS82_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0907075.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="PWS82_001977"
     CDS             complement(1827..3740)
                     /locus_tag="PWS82_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0907076.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="PWS82_001978"
     CDS             complement(3908..4414)
                     /locus_tag="PWS82_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0907077.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="PWS82_001979"
     CDS             4574..5014
                     /locus_tag="PWS82_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0907078.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5173..6513
                     /locus_tag="PWS82_001980"
     CDS             5173..6513
                     /locus_tag="PWS82_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0907079.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6582..7739)
                     /locus_tag="PWS82_001981"
     CDS             complement(6582..7739)
                     /locus_tag="PWS82_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907080.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7742..8098)
                     /locus_tag="PWS82_001982"
     CDS             complement(7742..8098)
                     /locus_tag="PWS82_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907081.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8396..9370)
                     /gene="sgrA"
                     /locus_tag="PWS82_001983"
     CDS             complement(8396..9370)
                     /gene="sgrA"
                     /locus_tag="PWS82_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL0907082.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9566..10405)
                     /locus_tag="PWS82_001984"
     CDS             complement(9566..10405)
                     /locus_tag="PWS82_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0907083.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10590..11477
                     /locus_tag="PWS82_001985"
     CDS             10590..11477
                     /locus_tag="PWS82_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0907084.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12061..12756)
                     /locus_tag="PWS82_001986"
     CDS             complement(12061..12756)
                     /locus_tag="PWS82_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL0907085.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12740..13138)
                     /locus_tag="PWS82_001987"
     CDS             complement(12740..13138)
                     /locus_tag="PWS82_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0907086.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3938 a         2652 c         2036 g         4530 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct
     5041 ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta
     5101 ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa ccattcaaag
     5161 gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta
     5221 aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga aacatgtttt
     5281 gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca ctatcaaaat
     5341 aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa tgatgtcagt
     5401 aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag
     5461 acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg cagagtcaaa
     5521 caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc acgtatgaag
     5581 cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccgtttct
     5641 ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt gaaaaacgtc
     5701 tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg
     5761 gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag aaaagtccgt
     5821 gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag
     5881 tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca catcggtcgg
     5941 gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg
     6001 aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt gaatggtcag
     6061 catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat agtcgatcgg
     6121 ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt ttttctttcc
     6181 gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag
     6241 ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac agaaatcaaa
     6301 cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa ccatatcaaa
     6361 gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga acgaatcttt
     6421 ttatatcgag gaaaatattt caagaaaact aagaacacta cccaactgac taaagccaga
     6481 acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt
     6541 gaatttcttc accaacactc attgacgaag agccgctttt cttaattaac gaaatccaat
     6601 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     6661 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     6721 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     6781 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     6841 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     6901 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     6961 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     7021 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     7081 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     7141 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     7201 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     7261 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     7321 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     7381 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     7441 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     7501 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     7561 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     7621 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     7681 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     7741 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     7801 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     7861 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     7921 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     7981 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     8041 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     8101 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     8161 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     8221 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     8281 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     8341 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     8401 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     8461 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     8521 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     8581 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     8641 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     8701 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     8761 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     8821 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     8881 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     8941 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     9001 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     9061 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     9121 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     9181 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     9241 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     9301 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     9361 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     9421 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     9481 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     9541 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     9601 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     9661 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     9721 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     9781 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     9841 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     9901 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     9961 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
    10021 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
    10081 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
    10141 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
    10201 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
    10261 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
    10321 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
    10381 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
    10441 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
    10501 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
    10561 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
    10621 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
    10681 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
    10741 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
    10801 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
    10861 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
    10921 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
    10981 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
    11041 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggggaaa
    11101 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
    11161 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
    11221 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
    11281 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat
    11341 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
    11401 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
    11461 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
    11521 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
    11581 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
    11641 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
    11701 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
    11761 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cctcttattc
    11821 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    11881 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    11941 catcgtttaa atgagaaata agtaatagtc acccaaactt taaattaatg aatattgaat
    12001 cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg agaaattgat
    12061 tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga aagtttgcac
    12121 ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta atacttgatc
    12181 tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg tagtgacttg
    12241 ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac gttcgatctc
    12301 agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca tcctgttata
    12361 ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta attctgtgta
    12421 aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg cttgaaggcc
    12481 tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta tgataactcg
    12541 agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga taggaatctg
    12601 tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc cttctttttt
    12661 cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac aagaataaat
    12721 actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca ttctgatcct
    12781 cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta tcttctacat
    12841 acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg atatgtccta
    12901 attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac atttgtttat
    12961 caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat tcaatcttat
    13021 aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt attttttctt
    13081 tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag atcgtcatct
    13141 ctgttcctcc tcgtaa
//
LOCUS       DANJJW010000074        12835 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000074 DANJJW010000000
VERSION     DANJJW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            6..305
                     /locus_tag="PWS82_001988"
     CDS             6..305
                     /locus_tag="PWS82_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907087.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWS82_001989"
     CDS             378..1739
                     /locus_tag="PWS82_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL0907088.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWS82_001990"
     CDS             1761..2021
                     /locus_tag="PWS82_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907089.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWS82_001991"
     CDS             2035..2787
                     /locus_tag="PWS82_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL0907090.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWS82_001992"
     CDS             complement(2881..3486)
                     /locus_tag="PWS82_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0907091.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWS82_001993"
     CDS             complement(3539..4513)
                     /locus_tag="PWS82_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL0907092.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWS82_001994"
     CDS             complement(4891..5610)
                     /locus_tag="PWS82_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907093.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWS82_001995"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWS82_001995"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0907094.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWS82_001996"
     CDS             7161..8558
                     /locus_tag="PWS82_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0907095.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWS82_001997"
     CDS             8639..9079
                     /locus_tag="PWS82_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0907096.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWS82_001998"
     CDS             9227..9553
                     /locus_tag="PWS82_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0907097.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWS82_001999"
     CDS             9554..10303
                     /locus_tag="PWS82_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907098.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWS82_002000"
     CDS             complement(10383..10838)
                     /locus_tag="PWS82_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0907099.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PWS82_002001"
     CDS             complement(11265..11540)
                     /locus_tag="PWS82_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907100.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PWS82_002002"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PWS82_002002"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL0907101.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4109 a         2210 c         2621 g         3895 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacag
//
LOCUS       DANJJW010000075        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000075 DANJJW010000000
VERSION     DANJJW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWS82_002003"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWS82_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0907102.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PWS82_002004"
     CDS             complement(322..891)
                     /locus_tag="PWS82_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL0907103.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PWS82_002005"
     CDS             1077..1979
                     /locus_tag="PWS82_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0907104.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWS82_002006"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWS82_002006"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL0907105.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWS82_002007"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWS82_002007"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL0907106.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWS82_002008"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWS82_002008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL0907107.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PWS82_002009"
     CDS             8547..8732
                     /locus_tag="PWS82_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL0907108.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PWS82_002010"
     CDS             complement(8794..9990)
                     /locus_tag="PWS82_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0907109.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PWS82_002011"
     CDS             complement(10160..11596)
                     /locus_tag="PWS82_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0907110.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="PWS82_002012"
     CDS             11889..12698
                     /locus_tag="PWS82_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0907111.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DANJJW010000076        12575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000076 DANJJW010000000
VERSION     DANJJW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWS82_002013"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWS82_002013"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL0907112.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWS82_002014"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWS82_002014"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL0907113.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="PWS82_002015"
     CDS             complement(2303..2965)
                     /locus_tag="PWS82_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907114.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="PWS82_002016"
     CDS             3149..4795
                     /locus_tag="PWS82_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0907115.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="PWS82_002017"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="PWS82_002017"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0907116.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="PWS82_002018"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="PWS82_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="PWS82_002019"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="PWS82_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="PWS82_002020"
     CDS             complement(8944..10023)
                     /locus_tag="PWS82_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0907117.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="PWS82_002021"
     CDS             complement(10126..10566)
                     /locus_tag="PWS82_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0907118.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="PWS82_002022"
     CDS             complement(10623..11033)
                     /locus_tag="PWS82_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0907119.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="PWS82_002023"
     CDS             complement(11213..11647)
                     /locus_tag="PWS82_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0907120.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12574)
                     /locus_tag="PWS82_002024"
     CDS             complement(11747..12574)
                     /locus_tag="PWS82_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0907121.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         4210 a         2339 c         2240 g         3786 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atata
//
LOCUS       DANJJW010000077        12205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000077 DANJJW010000000
VERSION     DANJJW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(37..216)
                     /locus_tag="PWS82_002025"
     CDS             complement(37..216)
                     /locus_tag="PWS82_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907122.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="PWS82_002026"
     CDS             complement(597..1304)
                     /locus_tag="PWS82_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907123.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="PWS82_002027"
     CDS             complement(1297..1734)
                     /locus_tag="PWS82_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907124.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="PWS82_002028"
     CDS             complement(1749..2000)
                     /locus_tag="PWS82_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907125.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="PWS82_002029"
     CDS             complement(2000..2206)
                     /locus_tag="PWS82_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL0907126.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="PWS82_002030"
     CDS             complement(2643..2903)
                     /locus_tag="PWS82_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907127.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="PWS82_002031"
     CDS             2978..3181
                     /locus_tag="PWS82_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL0907128.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWS82_002032"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWS82_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0907129.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="PWS82_002033"
     CDS             complement(3810..4709)
                     /locus_tag="PWS82_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL0907130.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="PWS82_002034"
     CDS             complement(4722..5315)
                     /locus_tag="PWS82_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL0907131.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="PWS82_002035"
     CDS             complement(5391..5540)
                     /locus_tag="PWS82_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907132.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="PWS82_002036"
     CDS             complement(5556..7760)
                     /locus_tag="PWS82_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL0907133.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="PWS82_002037"
     CDS             complement(7771..9231)
                     /locus_tag="PWS82_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL0907134.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="PWS82_002038"
     CDS             complement(9292..11664)
                     /locus_tag="PWS82_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907135.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="PWS82_002039"
     CDS             12074..>12205
                     /locus_tag="PWS82_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0907136.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DANJJW010000078        12170 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000078 DANJJW010000000
VERSION     DANJJW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(72..629)
                     /gene="frr"
                     /locus_tag="PWS82_002040"
     CDS             complement(72..629)
                     /gene="frr"
                     /locus_tag="PWS82_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL0907137.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(632..1354)
                     /locus_tag="PWS82_002041"
     CDS             complement(632..1354)
                     /locus_tag="PWS82_002041"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL0907138.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1490..2371)
                     /locus_tag="PWS82_002042"
     CDS             complement(1490..2371)
                     /locus_tag="PWS82_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL0907139.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2470..3252)
                     /gene="rpsB"
                     /locus_tag="PWS82_002043"
     CDS             complement(2470..3252)
                     /gene="rpsB"
                     /locus_tag="PWS82_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL0907140.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3611..4090
                     /locus_tag="PWS82_002044"
     CDS             3611..4090
                     /locus_tag="PWS82_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL0907141.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4307..5398
                     /gene="phnW"
                     /locus_tag="PWS82_002045"
     CDS             4307..5398
                     /gene="phnW"
                     /locus_tag="PWS82_002045"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL0907142.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5391..5519
                     /locus_tag="PWS82_002046"
     CDS             5391..5519
                     /locus_tag="PWS82_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0907143.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5523..6068
                     /locus_tag="PWS82_002047"
     CDS             5523..6068
                     /locus_tag="PWS82_002047"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0907144.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6522..8213)
                     /locus_tag="PWS82_002048"
     CDS             complement(6522..8213)
                     /locus_tag="PWS82_002048"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL0907145.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8633..9580)
                     /gene="arcC"
                     /locus_tag="PWS82_002049"
     CDS             complement(8633..9580)
                     /gene="arcC"
                     /locus_tag="PWS82_002049"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0907146.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9695..10714)
                     /gene="argF"
                     /locus_tag="PWS82_002050"
     CDS             complement(9695..10714)
                     /gene="argF"
                     /locus_tag="PWS82_002050"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0907147.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10805..12034)
                     /gene="arcA"
                     /locus_tag="PWS82_002051"
     CDS             complement(10805..12034)
                     /gene="arcA"
                     /locus_tag="PWS82_002051"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL0907148.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3334 a         2526 c         2201 g         4109 t
ORIGIN      
        1 ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc ccctctgttt
       61 ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat cttatctact
      121 tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg caaatcatct
      181 tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc acgacggatg
      241 ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc taattctttt
      301 ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc attggttgga
      361 ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat gctcttgtca
      421 aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat ttggttcaat
      481 ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc gttcgcacgg
      541 cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt cattttttct
      601 tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt gttccgatat
      661 tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag acaagcagtg
      721 gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt aatcctttgg
      781 caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct actttcggat
      841 cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct gcgccgattt
      901 ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg gtaccgcctg
      961 caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga atatatggct
     1021 ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc acgttttcca
     1081 acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg taatcagctt
     1141 gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt ccgccgccca
     1201 caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg atgatttctt
     1261 tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct ccactcaatt
     1321 ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata ttttctattg
     1381 ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt gttttgctta
     1441 aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat tattttttca
     1501 cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct tcgccaactt
     1561 cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt tcaacagtca
     1621 tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg aatttcttca
     1681 agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc aatgcttgtt
     1741 ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat tcgtttacat
     1801 aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt tcgtcagttg
     1861 ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat ccaccaaatg
     1921 cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt tctccgataa
     1981 cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg attttcattg
     2041 cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct tttactaaat
     2101 cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca acgattgctg
     2161 cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg tcatttttct
     2221 tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc atgtcgcctt
     2281 ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt tcgcgtagtt
     2341 cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg tttttctttt
     2401 taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa acagctccat
     2461 cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga tttcttcgat
     2521 tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt caccttggtt
     2581 tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac gaattgcatc
     2641 gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat caaccatagc
     2701 tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt tacgtgggtc
     2761 aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta agaatttttc
     2821 aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa cttcaaaagt
     2881 tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt ggatagtatc
     2941 ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc ctgcacgagt
     3001 tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata atgctacgcc
     3061 gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt taactgtttt
     3121 ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct tcatttttgg
     3181 attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt gtttcataga
     3241 aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct tatcttctac
     3301 cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg gatttgctga
     3361 tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag agcaaatata
     3421 cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa gaaaaacagt
     3481 agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg tataaaagac
     3541 gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc tttcgtaaag
     3601 gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca ttgaagaatt
     3661 cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg ttaccgccac
     3721 gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac ctgatgaaaa
     3781 tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta ggactgagga
     3841 agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac gagtccactt
     3901 tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg ttttagatga
     3961 gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca tggtcatctc
     4021 ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa ctgaagtatc
     4081 acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta ttttcaatat
     4141 tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt tattcaaatt
     4201 tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta acaagagcag
     4261 cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt ataaattatt
     4321 aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga tcgatcattg
     4381 tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat tattggatct
     4441 tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca gctccttcgg
     4501 ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga tttgttcaaa
     4561 cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga actataccgt
     4621 ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc tgattgaaga
     4681 gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg gtattttgaa
     4741 taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta tagtggacgc
     4801 gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg attttttgat
     4861 ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac ttgctaataa
     4921 agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc tatatgacca
     4981 gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta cccatacggt
     5041 tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg aaactcgata
     5101 ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga tggggttcca
     5161 gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc ctaaggactg
     5221 gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg tgatctatcc
     5281 cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga tctatccgga
     5341 agatattttg gaactgacag caattatcaa agactataag gagaacgtta atgattaaaa
     5401 ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct cctgtccacg
     5461 cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa atccgctaac
     5521 cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct tctgtcaaaa
     5581 aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag gaattgtatc
     5641 aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg aaacccgaca
     5701 cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc acgaatggat
     5761 ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg tattcaccag
     5821 acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct tatatgatcc
     5881 ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa gtcggagata
     5941 cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt gtaattgaag
     6001 gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg gggaaaaaga
     6061 tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata tttgtgtaaa
     6121 caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag aatgactttt
     6181 atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa cagccaagca
     6241 aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     6301 acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac agtcccctta
     6361 tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc attcagctga
     6421 ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct tttgtttctc
     6481 taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt atctggtgca
     6541 tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata aactagagct
     6601 aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata gtatttgttg
     6661 aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta tttttcccca
     6721 gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca gctggtatcg
     6781 tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc caacaggctg
     6841 actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa tcgaacaact
     6901 tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg gaaaatgact
     6961 gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg gtttttctcg
     7021 ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa taagacgatc
     7081 tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa tggaatatgg
     7141 tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa ctgtttaaaa
     7201 tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta agtacgttta
     7261 cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc tgacttctta
     7321 atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc tttatcctcg
     7381 ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc attgtagaaa
     7441 gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt attgaattct
     7501 ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc gccttcttcc
     7561 agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc agctacttca
     7621 tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac agcttcttct
     7681 gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca gtcacctaaa
     7741 tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat cgaattaccg
     7801 ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg agaagacatg
     7861 tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc tttttctttc
     7921 actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa gttcaagtaa
     7981 ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc taaatctgca
     8041 gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg aaatgcaaca
     8101 tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc cagtgttaaa
     8161 tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt catctttatt
     8221 cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag
     8281 tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac
     8341 cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct
     8401 tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac
     8461 aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa
     8521 aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa aggagaagaa
     8581 ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag
     8641 ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga
     8701 tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct actttcggaa
     8761 gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct gaaacagtaa
     8821 cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg
     8881 tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt
     8941 tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac
     9001 cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt
     9061 ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt
     9121 ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga
     9181 atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt
     9241 gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg
     9301 cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca
     9361 ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg
     9421 gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact
     9481 ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagatgg
     9541 cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag
     9601 ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct
     9661 gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac
     9721 ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt
     9781 gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc
     9841 gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca
     9901 agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag
     9961 gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat
    10021 cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt
    10081 gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta
    10141 attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag
    10201 agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc
    10261 caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag
    10321 tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac
    10381 tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct gtatcttctg
    10441 tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta
    10501 gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa
    10561 gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca
    10621 agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta
    10681 ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt
    10741 ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc cgtcaggaaa
    10801 caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacgac
    10861 ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt
    10921 cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt
    10981 ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga
    11041 tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat
    11101 gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt
    11161 tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat
    11221 ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt tcaaatcctg
    11281 aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag
    11341 aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat
    11401 ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt
    11461 tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga
    11521 ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg
    11581 ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg ctagaacgta
    11641 cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca
    11701 ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat
    11761 ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta
    11821 atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca aagttgtcat
    11881 gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat
    11941 ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc tttaatttcc
    12001 ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt
    12061 acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga
    12121 gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
//
LOCUS       DANJJW010000079        12132 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000079 DANJJW010000000
VERSION     DANJJW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            70..777
                     /locus_tag="PWS82_002052"
     CDS             70..777
                     /locus_tag="PWS82_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0907149.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            764..1087
                     /locus_tag="PWS82_002053"
     CDS             764..1087
                     /locus_tag="PWS82_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0907150.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(1176..2213)
                     /locus_tag="PWS82_002054"
     CDS             complement(1176..2213)
                     /locus_tag="PWS82_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0907151.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(2534..3517)
                     /locus_tag="PWS82_002055"
     CDS             complement(2534..3517)
                     /locus_tag="PWS82_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL0907152.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(3932..4702)
                     /locus_tag="PWS82_002056"
     CDS             complement(3932..4702)
                     /locus_tag="PWS82_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907153.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(4695..5630)
                     /locus_tag="PWS82_002057"
     CDS             complement(4695..5630)
                     /locus_tag="PWS82_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907154.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(5946..7595)
                     /locus_tag="PWS82_002058"
     CDS             complement(5946..7595)
                     /locus_tag="PWS82_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0907155.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(7712..10315)
                     /locus_tag="PWS82_002059"
     CDS             complement(7712..10315)
                     /locus_tag="PWS82_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL0907156.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(10327..11376)
                     /locus_tag="PWS82_002060"
     CDS             complement(10327..11376)
                     /locus_tag="PWS82_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL0907157.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(11402..11716)
                     /locus_tag="PWS82_002061"
     CDS             complement(11402..11716)
                     /locus_tag="PWS82_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL0907158.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(11732..>12132)
                     /locus_tag="PWS82_002062"
     CDS             complement(11732..>12132)
                     /locus_tag="PWS82_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0907159.1"
                     /translation="ASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGK
                     NIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILA
                     ELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         3497 a         2595 c         2053 g         3987 t
ORIGIN      
        1 aaaatttaga atattcacaa gtttgacgac aattatgaaa atttatgtcg tgctaaagga
       61 gttttgttat tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc
      121 gtttttggtt atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccgggttt
      181 catccgctgg tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc ccaatttatc
      241 acggtcagca tgcttgctag ccatagtcca ctgctctcca tcgttctctc gacctttcta
      301 gtcaattccc gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg
      361 ctgcttcaaa atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg
      421 aataaattga actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg
      481 atttcttatt tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt
      541 actgatccga aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt
      601 tatcttcaag tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt
      661 ctcactttga ttctggtata tgtcggactg atttttatcc caagtaactt agtgatttta
      721 gttgtaacac tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt
      781 tacattgctg actattttag gttgtacttt agcaacttgg ctttctagga tcctaccatt
      841 tgtcttactg aaaaaattcg atttgccgca accgttgctt gagtatttaa gttttgtccc
      901 gatcgtcatt atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct
      961 gccgcagatc aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct
     1021 ttcaaaaagc ttattggtga tcgtcttggt aggaattctt tccctttcgt tgattcgctt
     1081 attataaata aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc
     1141 aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca
     1201 acgacttttc ctaatatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc
     1261 gcattcatcc catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct
     1321 aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt
     1381 ttagcgaaca tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct
     1441 aacggctctt tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgtttg
     1501 atttgatcaa agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt
     1561 cctaattcac gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct
     1621 gtttttttca gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc
     1681 caatgcgcta gttgattcaa aatcgaaccg acgcccccag ctccgttgat cactaagatt
     1741 tttttcccgc ggttggcatt ttcttctgga gtcagaccaa atttttcaaa taacaattca
     1801 taagcggtca atgaggtcaa cggcaaggct gcggcttctt catctgaaag attttttggt
     1861 gcaagagcga caatacgttc gtcaaccagt tggtattctt gattgcttcc tgctcttgtc
     1921 gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc atcacctaca
     1981 gcaacaactt tcccgactcc atcaaaccct aagatacgta aagcatttct tatcccgtta
     2041 tcttggcgaa gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga
     2101 tcccgtgaac tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg
     2161 atcgacaacc cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt
     2221 cctccttagc ttcatcaagt tggagcatcc atacagtttt catttcaata tatatagtat
     2281 gctatttgtt ccacttttat tcatcataat tattctctta cttaaagtaa actatttgca
     2341 cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt ttaaacggtg
     2401 ttcaatcaac tgaccctcgg tattcgctca aaaaacgaca aaatatgagg agctattttt
     2461 gtttggctgt tcttattact tgatgcagga cagctttttc acgacctcta attcacggtg
     2521 ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtgtcta
     2581 agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg
     2641 ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg
     2701 ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt
     2761 ctttttctgt atgttccctc ggttcttttt caaatgcata aggattcttg aaaatccccc
     2821 ggccaatcat gatgccatct acgccatatt tttcggccaa ttcttctcct gtctggcgat
     2881 ctgggatatc tccgttgatc gtgatcagtg tttgcggagc gatttggtca cgtaaagcaa
     2941 tgattttagg gatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca
     3001 aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct
     3061 cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag
     3121 ccgcttcaat caattgtgcc gctacttctg ggcgaaggat caagccactg ccttttccac
     3181 gttctgctac attagggact gggcagccca tgttgatatc gattccttgg aagcccattt
     3241 ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa
     3301 ccatcggctg ttcatcttct gtaaaggtca atcttcctcg tacactgtct tttccatctg
     3361 gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat
     3421 gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa
     3481 aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc tccattctat
     3541 caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa
     3601 gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaagac tgtgacaaaa
     3661 atcttatcac aattcctttt ccggaataaa tggtgttcaa aagcttgcat ctgcggtaat
     3721 aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
     3781 tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt
     3841 attagctcaa caaacgacaa aatatgagga actattttcc cgtttgtttc tctaatactc
     3901 aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt cctttattga
     3961 tcaaatacca agtaacggcg tacaaaacaa cgatgaatag cacaagtacg atcatcgatg
     4021 ttgctactgg cacatcgatt gttccaagga atccgtaacg gaaaccggag atcatataaa
     4081 cgatcggatt gactttcgaa acagcttgcc aaaatggcgg caacatagag atggcataga
     4141 aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcgt
     4201 cataagactg agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag attgccgtca
     4261 tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg
     4321 aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cactccgcca atcacaaagc
     4381 cccaaatgat cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg tttttctgga
     4441 atttttgaga gaagaaggac gaagaaacgt tggcatacga actagtgata gcagacatca
     4501 taatcaaacc aggcacgatg aattccatat atgaaaaacc acccatgtca ccgatccgac
     4561 caccgatcat ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca ggcggaacca
     4621 gtgtttggac ccagatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg
     4681 cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc
     4741 aaaaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt
     4801 cttgtcagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt
     4861 gtctgttcat cttcaaatgt acaagcgtat ccttcaatca caggtttggt ctcatatggc
     4921 gctaaatcaa agataaatgt ttcaaattgc agtttggata gaagctcttt catactagta
     4981 ttctcgatca gttcacctga ttggatgatc ccgatgttgc gacaaagcat ttcagcttct
     5041 tctaaataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcggagaaac
     5101 tcccacatct cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc caagatcagc
     5161 aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa
     5221 agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg
     5281 ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt
     5341 tgctggaccg tttcaaaggg attgaaattg aactcttgcg ggactaatcc aatctgttgc
     5401 tttgctcgga ctagatccgt atccagatca taaccgaaga cattcacttt tccagatgtt
     5461 ttattgacta aagaagtaat gattccgatc gttgtagact tcccagctcc atttggtcct
     5521 aatagcgcat agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa tgcttcgacc
     5581 ccacttccat aaaccttttt caaattattt atttcaagcg catttgccat ttatttccta
     5641 tctccttata gacatgattc tttattgtat catggaatac agcagatgaa aaaacaactt
     5701 ggttccagcg aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa agacatcaat
     5761 cggacgaagt cagacaacct tttcagaaaa aacaaatcga agataaagga ttactattca
     5821 tatgtgcaac aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
     5881 cacgtataaa cggcggaagc aaaagccaag atttttgatc caataaatac tttcgatcac
     5941 ttcgcctatc ccaatacatg ccactgccgc ttttcatcaa ataacgcggc ttccgttttt
     6001 gcggtttcta ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat cgcatttcgg
     6061 gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc ttctgttact
     6121 tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa ttcgatatca
     6181 tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac tgaagcggaa
     6241 gaagcaatcc gttcgatcgt atttgtccct tttaaaatct catctacgaa gcagtagcag
     6301 cgttctttgg aagccacttg gtccaacaaa cgtttgatcg atttgatttc tgaaacaaaa
     6361 taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac atgtccaggt
     6421 tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat acagctgatg
     6481 gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac taatgtattc
     6541 ttctgccagt tgaccggatt cactaccgct tcttttaaca atggatgata gatatctttc
     6601 gctgtcacac cgccctcagt aaactctggt cggcaagtga tgggcatata agtacggtaa
     6661 ttcaatattg cagcggctac ttctaattct cccagcaatt cccacagctt gatggcttca
     6721 gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg cagcatgaac
     6781 atgatgttga catattcaaa aatcaactct gcttcactcc cactcttcat acgaaatgaa
     6841 aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc ttcttgaagt
     6901 ggtgtcggca gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa ataactcatg
     6961 ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt attgaatact
     7021 aacccaccga ttgccaaata gattcctagt acttgtccta aaagaagcaa acccaaaccc
     7081 accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt tttacttttt
     7141 ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg agcgaattga
     7201 tattggattt tttctctcag ttgaggattt tcctgataaa aagcaatcag tttttctaac
     7261 tgctgatctt caccaaaatc aaagctgcgt aaacgttgat acaacgcttc tgaacctaca
     7321 ctagaatagg tactgttgat tccttcgaat acttcgaaca tatccaggtc ataccaagta
     7381 agatcatcga tttcgctatc ccatgaacgg aacttcttct ccgtgagcca tgcttccttc
     7441 aagctttctt ctttatcaaa tctcggttga taaggaattt tttcccattt gctacgaact
     7501 gtttccttta atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat aacagctgca
     7561 ataataatta aaacgataaa ttctctttga cccatatccc tctccgttcc tttatttgtt
     7621 agctttagta tagcactgtt gcgctatttg aaaagagggt aaaaaaacac ctttcaaaat
     7681 aaaaggtgtt tttttcttgt atattacata tttatctctc atttatcaaa attgacaaat
     7741 aatcataggg aggaattgtt gttaattctt gtttattctc cagtgcattg acaactgtcc
     7801 caagggaaag gatagcctca ggtactattt tttcttgttc tgttggatta gctaagcgaa
     7861 gaacaaatcc ttgttcatga aaataagaag gataaatcga tgatacaatt aagtcttcag
     7921 gtaattcaat caatgaaaat ttacgaggaa catcaagttc attttgtaat ggccaaatct
     7981 tgttatctaa cctgtgaata aatacattca gtttctgctt ttgataagaa atagattgtt
     8041 ctaaccgttt ttctgcctgt tttgccactt tgaactcatt aaatcttcct tcttctacat
     8101 taaatccaaa agtgacttta tactcaccta tctcttgagc tagcggtgtt tccatcatga
     8161 catgcccttc atttgtcgta tctcctgaag ctcgcccagg tcgccaagca agattaggtt
     8221 ttccaagttc ccctgttgtt gaaaataagg ttatgattaa ttgattttct atacgttcat
     8281 actcttttaa accatctgca taaaacgtaa aataatgatc attttctgcc acagagacac
     8341 tcttatcgaa tatttctaaa ttaacaggtt tctctaccat agtttctgac caattcggag
     8401 gaatgacatc aggggtattt tcaataaatc cgttttgaat ttgtgcaatt gatttttcag
     8461 atttatctaa aacattaatt gctaagcgca tacgatgcga gtaaacagta ttatgaattt
     8521 tgagtgtgcc ttcaacgatt tgactttctc ccagactgaa cgatatttca taagatattt
     8581 cttccatttc gggtttttca gataaacgat cttttaaatc tttaggaagt tgagcttttc
     8641 cgtaaacaac taatgtttct tgtaaagaat ctttatatac tttagctgtt tctaatttta
     8701 gagatagttc tgtatctcct tctaatggtg aataatcata cgtatcgcca tcatttgctg
     8761 aatcgatcaa atgaacgaaa tcaagatatt gtctatctcc aactatcaaa ttcaattgcc
     8821 catctttaaa agaaagttta tatttattgt tacttatctc agttttgtct atctcgattg
     8881 ctgtttctaa tttttcatta tttttctcaa attcaacaac agtgtatcct agtgctggca
     8941 catttacatt tatttttatg tcaagttcat aataagctgg ttcatcaaaa tattcgaaac
     9001 ctgtagcaat catccgataa gcatttttcc gttctggaaa ataaacttct ttttcaatga
     9061 tattataggg actacctttg aagtgtattt tctttgaatc agctactaca tgcacgattt
     9121 tcgatccact ataattctca gtcgttgtgt tgaaaacaag gacttcattc gattttagct
     9181 ttaatgcatc agccatccgt tttacaataa ggttttcgat tccgtcagct atctcattag
     9241 cttctttgat tcgatggaaa atatcttccg caacattatc tgaaacacag ccaccaatac
     9301 tatcatgagc ctggttttgc aaaatctttt tccataaccg acgaagtagg cctttactga
     9361 tatggatacc atttctttct gcaatcagca tgagcggctc aactcgtttt agaatttttt
     9421 gttccaattt aaaattttct attttcattt ttgttcttac tgagccaata gaacgatgaa
     9481 cacgagagta aaccggctga cgaagttcac ctttatattc aggtaattct tttttgtttc
     9541 tgataacttc tacataagaa ggataatctg aaataatatt gtggaattta ctgtatttat
     9601 tcaacttctc tacgacttga tcaaaattta aaatcattga cttttgatct acacccgaag
     9661 gtactaaaat atctttgttt tttccttgca tatgaagcat ttcaactgct tcatccaact
     9721 tttcttttgc aaattcagga atatctttaa ttgcgtctaa ggtcatcatt ccagtcatat
     9781 aaccaaatgg aaaattcatg gcataaacaa atttatctcc caatcctttc cagataaaat
     9841 aaggagatgg aaccatcgtt tcaaagttta atcctcgcca acacatgaaa ttatcgattt
     9901 ctacttgatg caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca ggcaaatacc
     9961 ctaccatcat tggatctcca tatttaagga tcgtatcact gattccaatc attaagtttc
    10021 ttaaaattga ttcagcatcc actaataagg catctgtttg tgcataccac ggtcccacga
    10081 atagttgtcc tgttttcatc agtcgttgga taaccttaag cttttgcgga ttgatctcca
    10141 aatactcatc gacgattgaa gtttgtccat ctaaacaaaa gtttacttta gggttccttt
    10201 ccaattcatc tagaacttca gtaaagacct gatcagacaa tacgatggca tcttcatttg
    10261 taaaatacca ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt accatatcag
    10321 attctcctac tttccttctt ctactgaatt tacttgttgt tcttttttat acttgatgga
    10381 ttttagtgca ttgaccatta acgctgtaac cactccccca ataacaattg atactaaata
    10441 taaaggtata ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg tttcttgagt
    10501 aacaccaaaa aatccattta gaccacatgc tactgcagtg cccaccacga tgcttggtat
    10561 tactcgtaaa ggatctttca cggcaaacgg gatcgtccct tcagtaatcc ccacaaggcc
    10621 cataatcata gctgtctttg cattatctcg ttccgattca tcataaagtt ttttgtttaa
    10681 aattgttgct aaaaacattc caaatggcgg aatagcaatt cctaataaca tggctgtact
    10741 aggtccaaag cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga ttttattgat
    10801 aggtcctccc atatcaaacg ccatcattcc tcctaaaata gccgacatca aagcagcatt
    10861 tgctcctgac atgccttcta gccaattagt cagtcctgtt gtcattgctt gaataggcgt
    10921 agctagtaca taccataata tgattccaac aactaatgta gtaacaaaag gaatcagcat
    10981 aattgatttt agactaataa cttgcatcgg tacttttact tttttcaatg cattagctaa
    11041 atagccacaa gctaatccag caaccattcc tccaataaat cccataccaa gattttgaca
    11101 catcatccca gtgataaaag ctggtgctag accgggacgg tcagcaattg aatatgcgat
    11161 ataggctgaa atcatcggta cgataaactg taatcccgtc tgaccaatca ttcgcaggac
    11221 tccccagaat gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga ctgcaataga
    11281 agttagtaac cctgctgcta cgacaagcgg gatcatatag gaaaccccac tttggaaatg
    11341 atttagaatt tctcttcctt gtttcttgaa ttccattagt aactccccca cgatatattt
    11401 tttatttgtt aattaatgca attgcttcat caatcgtctc ttcaccatct tttactacct
    11461 ctgaggtccc tactgttaaa atcggtttac cgtcaaagcg acctcgttct gaaacatttg
    11521 tatctactgc aaatatgaca acatctgcct cggaaacatc cgaaattgaa agtttgtttt
    11581 ctatccccat agccccctgt gtttctactt taatatctaa ccctttcttc ttcgcaaact
    11641 tttctaaatt agcttttgcc atataagtat gggcaatccc tgcgatacat gctgttactg
    11701 ctactatttt tgtcatatga aattcctcct tttaaaatga tagagctttt accaattctt
    11761 ttttatttct cgaatctttt attcttttaa caaactcgtc atccatcaat cttccagata
    11821 attctgctaa aatacgtaaa tgattagtac ctttatcagt gtcttttatt gctaacaaaa
    11881 agacgagatc aactggttga tcatctaatg cgccccattc aacagaccgc tttaacttgg
    11941 ctacagccac tgttgattca attactgcta ggcttttgcc atgaggaatg gcgatatttt
    12001 tcccaattcc tgtggtcata tggttttctc tgtcaaatac gtctttttga aattgtttgt
    12061 ggtcagacac aaacccatta gtctctaaca atttcgataa tttttcgact acactttttt
    12121 tatcagatgc at
//
LOCUS       DANJJW010000080        12112 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000080 DANJJW010000000
VERSION     DANJJW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(129..716)
                     /locus_tag="PWS82_002063"
     CDS             complement(129..716)
                     /locus_tag="PWS82_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907160.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            891..2213
                     /locus_tag="PWS82_002064"
     CDS             891..2213
                     /locus_tag="PWS82_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0907161.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2368..3144
                     /locus_tag="PWS82_002065"
     CDS             2368..3144
                     /locus_tag="PWS82_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0907162.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3137..3706
                     /gene="rnmV"
                     /locus_tag="PWS82_002066"
     CDS             3137..3706
                     /gene="rnmV"
                     /locus_tag="PWS82_002066"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL0907163.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3699..4583
                     /gene="rsmA"
                     /locus_tag="PWS82_002067"
     CDS             3699..4583
                     /gene="rsmA"
                     /locus_tag="PWS82_002067"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL0907164.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5034..5663
                     /locus_tag="PWS82_002068"
     CDS             5034..5663
                     /locus_tag="PWS82_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907165.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5787..6896)
                     /gene="ugpC"
                     /locus_tag="PWS82_002069"
     CDS             complement(5787..6896)
                     /gene="ugpC"
                     /locus_tag="PWS82_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL0907166.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7105..7527
                     /gene="mgsA"
                     /locus_tag="PWS82_002070"
     CDS             7105..7527
                     /gene="mgsA"
                     /locus_tag="PWS82_002070"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL0907167.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7850..8386
                     /locus_tag="PWS82_002071"
     CDS             7850..8386
                     /locus_tag="PWS82_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0907168.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8426..10189)
                     /locus_tag="PWS82_002072"
     CDS             complement(8426..10189)
                     /locus_tag="PWS82_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907169.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10192..11907)
                     /locus_tag="PWS82_002073"
     CDS             complement(10192..11907)
                     /locus_tag="PWS82_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907170.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3783 a         2233 c         2467 g         3629 t
ORIGIN      
        1 aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa
       61 atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa
      121 aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag
      181 agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata
      241 ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag
      301 gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca
      361 aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt
      421 ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca
      481 accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg
      541 attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat
      601 ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa
      661 tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac
      721 tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc
      781 gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc
      841 tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca
      901 aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg
      961 gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga
     1021 cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg
     1081 aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg
     1141 gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata
     1201 ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg
     1261 attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag
     1321 ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg
     1381 ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa
     1441 tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt
     1501 tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg
     1561 aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat
     1621 tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg
     1681 cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg
     1741 ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc
     1801 cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa
     1861 agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga
     1921 tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca
     1981 gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc
     2041 catataaagt accactttat ccaatcgttc cgattattgc gattctcggt gctattttca
     2101 ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct
     2161 tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt
     2221 caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc
     2281 aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta
     2341 aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct
     2401 gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg
     2461 aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat
     2521 gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg
     2581 aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg
     2641 gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt
     2701 cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa
     2761 gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc
     2821 atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat
     2881 atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca
     2941 aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct
     3001 tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa
     3061 cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta
     3121 tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa
     3181 agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc
     3241 tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat
     3301 cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt
     3361 tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag
     3421 ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac
     3481 gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg
     3541 tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat
     3601 cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa
     3661 agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg
     3721 cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt
     3781 taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa
     3841 tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat
     3901 tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat
     3961 tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga
     4021 ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag
     4081 cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg
     4141 ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac
     4201 caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt
     4261 tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt
     4321 tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc
     4381 gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt
     4441 cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa
     4501 tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg
     4561 cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc
     4621 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg
     4681 ggcttgttac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc
     4741 cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta
     4801 gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga
     4861 cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata
     4921 ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt
     4981 gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa
     5041 aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc
     5101 caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc
     5161 cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga
     5221 catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg
     5281 tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg
     5341 gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc
     5401 attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa
     5461 catattattc tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta
     5521 tccacagttt agttgattat aatattgctt attttatagc aggaacactc agcaaatatt
     5581 ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt
     5641 acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat
     5701 ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt
     5761 ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta
     5821 tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg
     5881 aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc
     5941 gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa
     6001 gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct
     6061 tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta
     6121 agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca
     6181 ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt
     6241 gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt
     6301 gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg
     6361 gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc
     6421 attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt
     6481 ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct
     6541 gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa
     6601 gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca
     6661 atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct
     6721 tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa
     6781 ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa
     6841 taattttctg cattgtcata ttttttatat acgtttttca gagccatttc taccataata
     6901 cgttccacac tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac
     6961 taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta
     7021 agctttaata aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta
     7081 acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa
     7141 cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt
     7201 gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt
     7261 aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta
     7321 gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca
     7381 gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct
     7441 gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa
     7501 atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt
     7561 tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt
     7621 attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc
     7681 tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt
     7741 gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc
     7801 gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca
     7861 ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt
     7921 gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca
     7981 agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga
     8041 tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc
     8101 atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa
     8161 tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg
     8221 gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt
     8281 aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat
     8341 tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct
     8401 tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc
     8461 ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca atagctccct
     8521 gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa
     8581 aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg
     8641 tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc
     8701 gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct
     8761 tagtatctaa tttttccggc aaacgttcta caaattcata catataagag gcttttaggg
     8821 catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg
     8881 tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac
     8941 gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa
     9001 aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa
     9061 tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat
     9121 aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat
     9181 aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta
     9241 gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag
     9301 aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac
     9361 tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa
     9421 aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata
     9481 gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca
     9541 cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt
     9601 gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa
     9661 ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa
     9721 acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa
     9781 tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat
     9841 cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa
     9901 cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga
     9961 ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc
    10021 cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa
    10081 tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt
    10141 aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc
    10201 ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt
    10261 accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga
    10321 gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg
    10381 caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc
    10441 tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt
    10501 tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc
    10561 tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca
    10621 ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat
    10681 ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt
    10741 accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc
    10801 ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag
    10861 agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc
    10921 agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag
    10981 cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga
    11041 aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc
    11101 ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc
    11161 gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa
    11221 ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg
    11281 cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg
    11341 atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa
    11401 aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac
    11461 aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac
    11521 cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc
    11581 tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt
    11641 acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc
    11701 acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc
    11761 tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag
    11821 ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc
    11881 ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt
    11941 gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc
    12001 ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga ctattttttt
    12061 taaaaaaaag aaaatgagaa aacatgccag tctcttcatt cataggaacg tc
//
LOCUS       DANJJW010000081        12049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000081 DANJJW010000000
VERSION     DANJJW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(24..587)
                     /locus_tag="PWS82_002074"
     CDS             complement(24..587)
                     /locus_tag="PWS82_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0907171.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(602..1645)
                     /locus_tag="PWS82_002075"
     CDS             complement(602..1645)
                     /locus_tag="PWS82_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907172.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1778..2182)
                     /locus_tag="PWS82_002076"
     CDS             complement(1778..2182)
                     /locus_tag="PWS82_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0907173.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2415..3050)
                     /locus_tag="PWS82_002077"
     CDS             complement(2415..3050)
                     /locus_tag="PWS82_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0907174.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3316..4368)
                     /locus_tag="PWS82_002078"
     CDS             complement(3316..4368)
                     /locus_tag="PWS82_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907175.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4381..5319)
                     /locus_tag="PWS82_002079"
     CDS             complement(4381..5319)
                     /locus_tag="PWS82_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0907176.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5770..6420)
                     /locus_tag="PWS82_002080"
     CDS             complement(5770..6420)
                     /locus_tag="PWS82_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907177.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6520..7029)
                     /locus_tag="PWS82_002081"
     CDS             complement(6520..7029)
                     /locus_tag="PWS82_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL0907178.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(7230..8099)
                     /locus_tag="PWS82_002082"
     CDS             complement(7230..8099)
                     /locus_tag="PWS82_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0907179.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8119..9240)
                     /locus_tag="PWS82_002083"
     CDS             complement(8119..9240)
                     /locus_tag="PWS82_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL0907180.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9242..9637)
                     /locus_tag="PWS82_002084"
     CDS             complement(9242..9637)
                     /locus_tag="PWS82_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0907181.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(9656..10462)
                     /locus_tag="PWS82_002085"
     CDS             complement(9656..10462)
                     /locus_tag="PWS82_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0907182.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10455..11243)
                     /locus_tag="PWS82_002086"
     CDS             complement(10455..11243)
                     /locus_tag="PWS82_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907183.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11257..11733)
                     /locus_tag="PWS82_002087"
     CDS             complement(11257..11733)
                     /locus_tag="PWS82_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907184.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(11805..>12049)
                     /locus_tag="PWS82_002088"
     CDS             complement(11805..>12049)
                     /locus_tag="PWS82_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0907185.1"
                     /translation="LGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQ
                     KFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         3752 a         2579 c         1820 g         3898 t
ORIGIN      
        1 aatggatgct aggtccgtta tatttactca aaattgattc tacgaataga tcttcttatc
       61 atttctattt tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg tataagtagt
      121 ttagataaat ttgtaccatt tacatttggt tgcccagttc cgtaagaaat tttttctaat
      181 aacttccaat ataatggcga gtctaagaag cagtctacgt attctgataa taatttctca
      241 actaacctaa ttcgaatcaa gtaagaagca aaaacagatt cctcacttat ttccttaacc
      301 aagaaacttt ttcccatagt tcctcctgtc cttgctatca aaatgtcatt ttcttccaat
      361 cttaaatcaa ctagtttaga attactaata tcacagtaag gcacactgga ccaatttaca
      421 cgtccctctt gtatatctgt gattctcaaa aacttaacat tgccttgttt tttcgccgaa
      481 tctgtatagc catattgaat cttagtagaa atacttccta aataaaccca tagtcgaaca
      541 tcgctagatg atttttcttg tcgttcttta tgaacaacag tgatcatctt gagttgtccg
      601 actatttttt agttgcagca atctttttgg gacgcttatt tgcatgaagc aacgttgcta
      661 agtctagtaa taaaacattg gctaacacag gattggcttt ctcgcgatac gctgcggcgc
      721 cactttcaaa caatactgct agacgatctt ctacaacaac gatttgttcc aaatacggtg
      781 gaaatttgat tcgttttagg atccggctag gagagtcccg ctcggacact aagagttccg
      841 aggtataagg accataagaa cgactggcta agagataatg gccataaaaa gccacacctt
      901 gaatcttatc agatgttttt ctgctcattg tcacggttgg ttggattttg tctttgcggc
      961 gaattgctgt tttcatttca gctatcgtcg gcaatagata gaaagtcaac tgtccttcac
     1021 cttttagtgt gaagttgcca gataccagtt gatcattgtt ttgagtcaaa tacgatgctt
     1081 gtggaatctc tgagagatca gttgtcagcc aataagcgat tggctgtccc gtctcttcag
     1141 atgtatctgc taagatatcg atcaaacgta aagcggctac tcgtccagta gcagatcctg
     1201 tgattgtcaa ccataaaagt tcccgttttg tatcatatac taagccccct acatgtggcc
     1261 gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc tctatccagt acatgaataa
     1321 tagaatgatg ctgatggtca tagcagtagg cagaaataaa cacaaaatct tgagaaaaag
     1381 ccaacccttg cggcgtcatc cattgacaaa cctcattttc gccggtttcc ggcaccacca
     1441 ttcttgtttc tgttaatcca ggaatgttat attgttttgc ttcagccttt tcccaagctg
     1501 cttgaacaat aggataccgt tttaactttt tttcaatatc ctgatgtatc cgttgctgct
     1561 tccaatgctt aaactttagg acatagtaaa caagaaggcc aatgaccagc aacactaaaa
     1621 tcattattgc tacccgctgt gtcatttttt tgcctccttg ttctattcga tcaatcgtcc
     1681 atataccgct attaattacc gtaactacat gtttctttta ctagcgtttc tttataggat
     1741 ctttcataat ctcatttata gtaacattaa gaatccctta ttctactata gtcacttcca
     1801 catcaatatt gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct tcttctgcga
     1861 ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg accccgattt
     1921 ggtagccccc atcttctaat tgaaataaag atacccgtgc tttgacttca gaatcagact
     1981 caagattatt ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat gcgtatccag
     2041 ccgcaaaaag ttgttccgga tttgtccctt cactatggat tcctgggcgg tcgatcttca
     2101 tgtgcatact tccgtttggt gcctcaactt cgccatcacg gcctccacga ttgatcattg
     2161 ctgtttcgta catttttttc attgagatca tcctttcttt cgtcgttcta tattaagtgt
     2221 aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc agttagacaa
     2281 catcgatgat cagtgataga agcagtttat ctatacaaaa aaagaagggt tctatgatat
     2341 ttggtgtcct tactcgcctt gagtaacaac accaaatatc atagaaccac ttaatcagct
     2401 tcacactgct ttttctactt tcgtagttga aaaaaactta aacaaatcat cggcaaatag
     2461 ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct taaagaaggc aaactgacta
     2521 atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat aactgttaaa
     2581 acaagataaa aatggctaag cgtaccttga ccactttgac gataaagcag aacaagtaac
     2641 aatgggaaaa gtagaaagcc tgcatagcct agcccagaac ctatattatg gatccaagtc
     2701 gatagattac aagagctttc attcgtattg atactgaata agccagtaaa aatacaatca
     2761 cctatcccat ataaaccaac tgttgcaaca acaagtattg caagtgtttt tgatgttcca
     2821 acaaataggt gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga tacaactgac
     2881 cagaccaaga aagcacgtct gactggacta tctacgtcac caaacacact aattacggta
     2941 gtcatttgat tcagtttggg ataaaaaagg ccaaggatat acggtgtcga taaatcactc
     3001 atcacaccta ataataaaaa ataaaaacca tatttttgca aaaaaatcat tttcttcccc
     3061 tactttttag tgattattcg atacatccaa caatcaaatt ttttctcatt ttaacacata
     3121 tttttattct agttgtcttt tgaacaatca ataacataaa ggtgagtttc ttctacattt
     3181 taaaaaatcg aatggcactg aagagttcag ttgtcagcat caatctccta ctcaaaaagg
     3241 caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt
     3301 tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa
     3361 tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca
     3421 aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc
     3481 gaccacgccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat
     3541 agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt
     3601 aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac
     3661 cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc
     3721 agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat
     3781 aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt
     3841 gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata
     3901 aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga
     3961 cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt
     4021 attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt
     4081 gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg
     4141 atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctagttgaaa
     4201 aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat
     4261 ggcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag
     4321 gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct
     4381 ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata
     4441 aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat
     4501 ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat
     4561 tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat
     4621 cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc
     4681 agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat
     4741 tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc
     4801 atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc
     4861 acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc
     4921 aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta caaatttctc
     4981 aataatatct tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat
     5041 taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat
     5101 cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc
     5161 tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc
     5221 tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatca acccaaagcg
     5281 acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt
     5341 tctattatac tcatttgaga atcttttcac aacctcgttt tgttattctt ctcttattta
     5401 tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga
     5461 atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga
     5521 aaaaatctca ctcctctgat tataaaagag ggggcattaa cctcgtgtat actaaaacct
     5581 tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt
     5641 gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat acacttattt
     5701 aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat tatcatgaaa
     5761 aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt tattaattag
     5821 ttcatcgccc aataatctct ccagtttctt agcatctcct gactcaaccg gaattatcca
     5881 agcccagtct aaataatttt ttaaaatctc atcatatttt gcaaatattt cattgccata
     5941 ttccgtcctt aaaattatat tttcagtgcc agacaagtga tttatattga gttgataaaa
     6001 aagatctcca tctaaaaaaa tatcattaga agaagatttc cccacagtaa tgaccattcc
     6061 atcagctcta tgtgttacat acaaataatt gtatgacctc atttcaccta atgaatcaat
     6121 tatttcttgc cctttagctc taatatattg atctctatta cctatctcac tcttatttaa
     6181 caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt cgcttctact
     6241 actaatccaa tccgagatta atttttcaac atgtcccaga gtaaactctg atttattcgc
     6301 taaatacttt ttttgttgta ttaaagacat ctttccccga agctcaaaat tgagcctaca
     6361 tcgattctct atctcttcaa aaagcatctt atcttcatca ttcttatatt catcattcat
     6421 aaaaccaatc cagctcctcg attaagataa tattttaatc tcatacaaaa actagctctc
     6481 atcaaaacaa ccattgtttt gatttctcat gaaatttctt tatacttttc tccgattata
     6541 aattcctaac cccttgagaa aattacgcat atatttatct ccgcaaggct taaaatttcg
     6601 ttggccttct tttctcaaaa atgccccgat ttcgcctttt gatggataga tttctgcctc
     6661 agcaaaaacg tctaaaatat catcagtggt catagacaag gcgattttta atttttttag
     6721 caacacattg ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta ctatcggctt
     6781 atctttttgc ggaccacgcg ccaagagaat caggccattt aggaatgcgt caaaaacttg
     6841 gttgttgatg gttttttcat acacattatt ttctgcatta tctttgttct tatcctgtac
     6901 tttggtcagt accgcctgag cttcttcttt agtcagtggt acaccgccta gctcaaaagc
     6961 tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc aatcgcaata acctatcatt
     7021 gttattcatt tgttcctccg aagtatttag tctttacttt cttacgtact ttttgtcttg
     7081 ctaagatttt taggatcaat gataaaaacc gcatcactta tgataaggcg ctgggtatca
     7141 tgagtgaatg atattgctga atacaaatag tccgaccaca ttaagttgat cggacttcat
     7201 aagcatttta ttgactaata cttttttgct taagaattga ttctaacatc ttttgctaaa
     7261 gttaagcatc ctatagttcc tgcattatct gctaacttag gtgtgacaat gtattcttcg
     7321 atatttggcg tatcgacata gccgttaatt agttctaaaa aacgtctttg tactttcttt
     7381 ttcagatgtt cttgttgcat gacaccacca ccaaaaatta taatatctgg tgaaagcatc
     7441 agggtcgtat tacaggcaca ttgtgcaata taatccgcct cgatttccca gaacggatct
     7501 tctatagaaa gatcttgtcc cttggttccc gtacgcatct cgatagcggg tccagatgcc
     7561 aacccttcga gacaatcatg atgaaaagga caagtaccag agaaagtatc tcctgaatgt
     7621 ctcttaacta acgtatgccc catttctgga tggctaaacc cttcaacaaa aatcccatct
     7681 tgtatagctc ctccaccaat tccagttcca acagtgtaat aaacaacact ggataaacct
     7741 tttccatgac ctgaaacata ctccccgtaa caagcagcat tcacatcggt tgtccaggca
     7801 attggaattt cgaaatgctt attcaacgtt ccaacaaaat caaaattttt ccaggcagtt
     7861 tttggagtag aggtgatatg tccataggtc tttgattcac gatggatatc aatcggtcca
     7921 aatgacccta ctccaatgct ttctaattgt tctttatagt gattaaagaa atcaattacc
     7981 aaactcattg tttcattcgg tgtagttgta ggaaaactca ctctttctaa aacctttaaa
     8041 tcatcattgc caatggcaca aacaaattta gtcccacctg cttctattga gccatacatt
     8101 tcttactcca ccgtcctttt atctaggaat tcgttcttcc ctgtctaata gtttttcgaa
     8161 aaactcatgt ggttcctttt ggtaccaata ggcaacagaa gaaatatcgt cttgacgctc
     8221 ataaagcttg atgtcatcat ttcctagggt ttggaaagta acacgtaaat tttctttgaa
     8281 actaatgggg tcgggtaaat gccagcggta caacccatgc atcggtaaag catcatcgcc
     8341 aaaaccatga actggatttg gattaggttt tccagcacta aaataatccc gggtgtgatc
     8401 tcttttagat tgaaaaggat agcccataaa tagcgtatta aaattttgag catatgccct
     8461 ttctccaaag ttctttttat ggaaagccca agcgccgcca aaataatctt ctgctcctgt
     8521 agatgttatt gtagggaaat cttcatctcc atctatataa aacttaaatt ctccttcccc
     8581 ccaccagtat ctttccaatg cgcacaaagc aaagtaggtt cctacataat acccagtgcc
     8641 tttgattcca tcaataaggg tgtaatcttt tccttttgtc gtgatgggtt cacggttcca
     8701 tttagcatga aaatacatcg catcatccgg tacttcagtc aatgcataat taaatgtata
     8761 aaaaatgtgc ttaataaact caggatgttc attcgttaaa gtaatcttag ctttttttcg
     8821 aaatggcatc tcaaaatagc aattcatccc tgatgttgga ttcactacga ttggtaagga
     8881 attcacttcg catcttgtcc caaacccatt acaaaaaaaa tctcctaatg gactttctac
     8941 agaaggagtg gattcatcat cccaataaat acgcaaaatt aaatcacgta agacgaaact
     9001 cccagcgatt gttttgtctg tcactgtcat ccaaaaatga cgtaaaattc cagggccttc
     9061 catttctgct aaagtaattg tctctccagc atttatcgga acagaaggtg acccttttct
     9121 cccaattcct agatgactcg ctgtcattgc cgctttccct ttttcaccat ttttattttc
     9181 tggtgaaata gttctacttt cctctccctt gtggagccat atattattta aacaattcat
     9241 tttattcgtc ctctatcctt tcttcacttt tttgattatt catatagatg atttgttgtt
     9301 gtgcctccgc aataattgct ccaatatcta aattatcatt tcctaactgc aaagcgattg
     9361 acattgccaa gggaagattc attcctgcaa ttacgtgcac tctgtcagaa atataggtaa
     9421 aaaacttttg atttacactt cctccagcca tgtcagttaa aacaattatt tcgtctgtag
     9481 tatccactaa ctctttaatt tgttcatcta tatcatcaat ttcttcgaca tatgcacata
     9541 agctactaat ttgattatgc atacctgtta aaaactctag tgtctcttta aaaccctcag
     9601 ctagtattga atggctagca atgatcaatc ttctcataat tcaaatcctt ttcctttacg
     9661 ctaatattcc aaatgcagcc ataaccatcg caaaaacaat aacgagtaga atcaaaatgg
     9721 tcatactgat ttttttctta ctgagtagat aatatgctgc agcagtaata attactggaa
     9781 ttaatgttgg taaaattttg tccaacattg gttggatttt catactaact tcaccaaaag
     9841 tgaatttaag tggcgttgaa acactgataa ctgaagcaat taatgatcca actactgtta
     9901 gccccaatac tgaagcagct tcagttaaat aattaatctt ttcaccgtat tcagtaacaa
     9961 tcttcattcc ctgcttataa ccaaattcaa agcctctcat tcggaaaaag aagattacca
    10021 tgattaaaat gaaccataaa aagattccta caggattatt ttctaaagcc atataagccg
    10081 caattgatcc catgatggtt gggatcatcg tcatcaaaag agagtcccca ataccagcaa
    10141 ttggacccat cgtaccaact ttaaagtcat taatcgcatc ttttgcttct aatccttgct
    10201 tttcctccat tgcaacacaa gcacctaaaa taatggctgc cagccaaggc tgtgtattgt
    10261 aatactgaaa gtgattattt aaagcttcga tgtattcatc atcatctttg tataattttc
    10321 ttaaaatagg tgacaatgcg taagtcacag atgcaccttg ctgtgtttga taattaaagg
    10381 tacaaactga catcaaccaa cgatagttcg ctttcctaag atcctttttc gttacttgat
    10441 atttttcgct cattttattc atcctcccca ataaacccaa catcagaagc tcccgcagaa
    10501 gcagtgattt gtgcatcttt ttcagctcta tgatacacat taaatgctaa agcgaatcct
    10561 atgatcgccg ctccaataat aggtaccttc aaaaaagcgg ctaagaaaaa tccaacaagt
    10621 aaataactaa tgtatcgacc tgtaggcata aagcgtaaca acatcgcaat accaacagca
    10681 ggcaacattt ttccggctaa tgttagccca tccataaacc aattaggtaa aacatccaaa
    10741 acgtaattaa cagcagactg tcctaaagta acggcaataa atactggaat tgctgcagat
    10801 aaaccaaaga aaattggccc caaccacaaa acactattca ttttcttaaa ttctcgttta
    10861 ttcgcatatt cctgtgattt gcgaacaaca aaaccattta agattttgac aattacatct
    10921 agttgcaccc ctaacatacc tacaggtagt cctattgcta atccggcttc tactccaagc
    10981 tggctaccaa atgccgttgc aataattgcg cacgttccat agtccggtac agatgagcca
    11041 ccaaaaccgg caactcctaa tgacatcaat tgaactgtac cgccaactat caaacctgtt
    11101 tttatatcac ccatcaaaat tcctgtgaat aatccccaaa aaactgcgta atttacaaag
    11161 atcatcggaa tcccataata atcaacatgt ttaataaatg ctaatatagt cagcataata
    11221 atttgaaaaa tagtaaaatg cattatcctt actcctttat cctaattttg attgaatttc
    11281 ctgtgcctta tccgccggaa caaattgtgt aacaatttct acaccagaat ccaacaattt
    11341 tttatacgtg ctgatatttt catctgatgc ataggctcta gaagatagct gtaatgcatt
    11401 atctttaaca acagtaccgc caacaattag tcggtcaatt ctaataccgt tatctactaa
    11461 atgaaggatt gtttccggct ctttcacgat gacaaatact ttttcctgat cgtatttccc
    11521 ctttttaaaa ttcgcaatcg ctgtttcttc agtgataatt gatacagcca ttccagcagg
    11581 tctagctaaa cgcatacttg atttttggat gtcgttattt gcgacagaat catcgatcac
    11641 catcactctg tttgctttag acattggtgc ccattgcgtg acaataatcc catgcaataa
    11701 acggttatca actctcgaca taacaatact cataaatact tcctccttta tctttcattt
    11761 tcttacaagc aattaccatg ccaaaacaaa aagaacttga taaatcaagc tctttttaca
    11821 taatcataaa cgtatttaac ctcatgaatc gggatgtcga cactataata atgttcaacg
    11881 acactgaaag attgcttaaa atgtgtctga aatttttgaa attcctcact cgtttcattg
    11941 aaataaattt catcttccaa agtattttgt tttactaatc gttcaattaa gcaactaatg
    12001 tgaacatata atccgaaaca cgtattatta tcaaatgaaa ttcctagat
//
LOCUS       DANJJW010000082        12010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000082 DANJJW010000000
VERSION     DANJJW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(159..1622)
                     /locus_tag="PWS82_002089"
     CDS             complement(159..1622)
                     /locus_tag="PWS82_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0907186.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1686..4703)
                     /locus_tag="PWS82_002090"
     CDS             complement(1686..4703)
                     /locus_tag="PWS82_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0907187.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4854..5693)
                     /locus_tag="PWS82_002091"
     CDS             complement(4854..5693)
                     /locus_tag="PWS82_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0907188.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5838..6083
                     /locus_tag="PWS82_002092"
     CDS             5838..6083
                     /locus_tag="PWS82_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907189.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6146..6568
                     /locus_tag="PWS82_002093"
     CDS             6146..6568
                     /locus_tag="PWS82_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907190.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6662..6979)
                     /locus_tag="PWS82_002094"
     CDS             complement(6662..6979)
                     /locus_tag="PWS82_002094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907191.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(7006..7212)
                     /locus_tag="PWS82_002095"
     CDS             complement(7006..7212)
                     /locus_tag="PWS82_002095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907192.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7193..7375)
                     /locus_tag="PWS82_002096"
     CDS             complement(7193..7375)
                     /locus_tag="PWS82_002096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907193.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7389..7739)
                     /locus_tag="PWS82_002097"
     CDS             complement(7389..7739)
                     /locus_tag="PWS82_002097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907194.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7765..8067)
                     /locus_tag="PWS82_002098"
     CDS             complement(7765..8067)
                     /locus_tag="PWS82_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907195.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8070..8303)
                     /locus_tag="PWS82_002099"
     CDS             complement(8070..8303)
                     /locus_tag="PWS82_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907196.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9289..9611)
                     /locus_tag="PWS82_002100"
                     /pseudo
     CDS             complement(9289..9611)
                     /locus_tag="PWS82_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9734..10849)
                     /locus_tag="PWS82_002101"
     CDS             complement(9734..10849)
                     /locus_tag="PWS82_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0907197.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10855..11253)
                     /locus_tag="PWS82_002102"
     CDS             complement(10855..11253)
                     /locus_tag="PWS82_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0907198.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3215 a         2444 c         1837 g         4514 t
ORIGIN      
        1 cgataaaaaa tattcctaat actatattta ataaactacg ttctagatag atacgagatt
       61 tcaaattaaa aaacgaaaat gagagataca aatatatcaa atatttaaaa agtaaccaca
      121 ttttctatac cactccttat cagaaacagc cttattcttt agaataagac tgtttctggg
      181 ttgattaatt tgtcttttgt taaactacca tctaaggcaa gttctaaatg taaatgtggt
      241 cctgtactat ttcctgttga acccacataa ccaataattt gtccttgttc aactgtttgt
      301 cctacgttca cgcaatattc ctgttggtgc gcataaagag ccacacttcc gtcttcatgc
      361 tcaattgtga cataatttcc tgacaaagta tgatacgaag cttctatgac cgttcctgcc
      421 ttgctcgcat agattggttc tccttgattg gctgccatgt ctaatcctct atgaaattct
      481 tctcctcgcc aaccgaagga actagaaatt gaataatttt ttagaggtac catatagcct
      541 tctttgctta gttgtggacc agctacttct ttatcatatt tgattaagtc ataggtttcg
      601 attaacccat ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc tgttaaaaat
      661 ttagtcgctt cctgataggt ttttgcctta cttttccaaa caccatcata aaaagaggta
      721 tttccaacta agccttcttt taacagtttg gcatagtctt ttaagctttc ttcatagctc
      781 tcatattgcc gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa atcttcttgt
      841 gtcgcaaaag agacgccctt tccgttatgt gtgcctttga ttccaaatag attgtagttt
      901 ggtgcctgag ccaattgact ttgtccgctt gcggattcta aaatggcttg cgcgatcata
      961 acagaagcgt ataaatcatt ttcttgtcca attttacgag cagattcacc gattttacgg
     1021 ataaacgttt ctacagacgc gtctttttca aaatgaatag tcccttcgtt atcttgtgtg
     1081 ctggaaggga ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt ttttgtctct
     1141 tctgaaccga ttgattgtgc ctgcggtacg tcattcatag gtacagtcac actctgttca
     1201 ggtgcctttt gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc ggtacttggt
     1261 ttttgaggag tctgtgagct ttcatcagaa ggttgctcat ttggttcttc tgacggttcc
     1321 tcctcaggtt gttgattgtc ttctggtttg tctggttgat tcggtgtttc tggttgttct
     1381 tcagaacctg ttggtggagc gttcatttct ggattatctg gtgtatctat tgtatcttca
     1441 gtcggtgttt ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc ttcagtgatc
     1501 gttgcagatt gcgaatttgt cagttgattg ttgccttgtt cattagccaa aaccgctggc
     1561 gtatacccaa atacttgacc tactaataac aaagcagata aacatttact cgtttttttc
     1621 acaagattgc ctccattctt caatcaatca tctagagaca gctgattgct gtctctagtc
     1681 atactttaat tgctttgttt cattttacga cgaaccagaa cagcccccac accaacaaaa
     1741 agaaacagtc ccaatgcaat taacacatcg ttcactgttt ctcctgtttt tggtaagctt
     1801 tgttttggtg tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc ttttttaatg
     1861 ccttttgctg tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc ttcttcctct
     1921 ttatggtctt tatccttata gacaatatga gttgcgacca atttctcccc gtcttttaac
     1981 gtgcctgctt tgtagtcaaa ggatacttta aactcttcag acgctttaga aactttacgt
     2041 gcagtttcta ccgtaccaac gactgtatct tttccatttt tatcaatctt atggaattga
     2101 gtcacccagt atttggtttc ccctaccgta gattctggaa attcttgcga tacatgatcg
     2161 atcaactctt ggtctttgtc tggatcaaat gtttggtcgc cattcactgt aataaagcgt
     2221 gtttcgattt ttggttcttt cgcagtcact gtttgatctt tattggttaa atcaaaatga
     2281 cgagcgaatt cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc gacaatcgtg
     2341 tcaccgtctg aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt ttctttttca
     2401 tcggtcactt ttcgtgtttc ttttaccgta tctaatacag tgacttttcc atcggcagta
     2461 attttatgga attgcgtcac ccaatatttc tctgtcccaa ccgcagattt agggaatact
     2521 tgggacactt tatccactaa ttcttggtct tttttcgggt tgaaatcttt tccacctgtc
     2581 gttgtcacaa acaaagtaga aatttctggt tggtcttctt ctggaaccac gatttcttcg
     2641 tttttcacag taattggttg tacttctccg tctttgaccg ttactaaaat cgtttctgtt
     2701 tgtcctgacg cttgcccata atgctcaggg aacacaattt ccgtaaattt atacgtaccg
     2761 tctggtaatt ttagtttttc tggttcttct tttgtttcat acacaaagga cacattttta
     2821 ttatgtgcag tcataccttc aatcaggaat ttcgctcctg gtaattgtag atgattgatt
     2881 gaatcgactt tattgaagtc tacttcaccc gtttttaaca agttgtcaat cacatctaat
     2941 tcaaacgtaa gcacaggcgt cgtgttatcg ccataggcca cagagaacgt ggtttgttct
     3001 tcagaaggtt gacggtagtt gtctggcaca tttttttcaa tcaattggta tttgttttga
     3061 tcgccattgt agaatttttt gtttgccttc cagtgtccgt ctttgtcagt ttttactgaa
     3121 tccaagactt ctcctttttt cacaaggact gtttctttat ctggcgcagt aatatctgtt
     3181 ttggcgataa tatcaaattc gatattttct gcaggaacgg tatcataaac aggtttgcct
     3241 tgattccagg attcttccgc attatagact tctttgactt tctttccttc tggaatgaac
     3301 tgttgtaagt cattttttgc agttaacgtc acttctttgt ttggttcaat cgtaatcgta
     3361 tgagtttgtt tgtctagaat atacccagct ggtgctttga cttctttgac atccaattta
     3421 ttttgaacat attttccgtc tttttcatag aatgcaggga tttcttttaa ggcgatttcc
     3481 cctttggcat ccgttgtata ttgttttgtt tctcccgtat catgacgttt gatttcatac
     3541 gtcacatttg gaactttttt ccctgtgaca gcgtctagtt tggttagttt gaagtcgcca
     3601 ttttttagaa ctttaatagt tagtccagct ctattaggat ctggcacata aagctttcct
     3661 agattttgag caacaggatc tacataggca aaacttgaac ctacccctct agaatttgga
     3721 tccagtgaag aaacagaaat tcgtccgtca tttgagtttt gatttgcttt tataaacaaa
     3781 ctatttcctg attttcttac ttgaacgtta gctgtgtttt caacaaccga cccatcccat
     3841 tgagaaaaca caccttttgt atccttaact tctacttctt cccctacttt aaccgtcact
     3901 gtttggctat caaaagaagg atcttcaagt aaattgttta tttttctatc tacagtctgt
     3961 ttgaaagctt cataattaga ttgtccaata gttccataat taacaaatga accacctaaa
     4021 acttcccaaa tatatgtttg tgctaataca tagttatcca ttgaaccact agaatagtag
     4081 ccaaaataac caattaaaga aatctttttt ctaatagcac ttgtcgtaat taaattttcg
     4141 agtggtgtca catcatattg aatacccgca ctcgtgattg cgccttcttg aatgcaaaaa
     4201 acatcttctc cgtcaatttg ccagcgaacc atgtaatcat gagtatacat tgttccttca
     4261 ggagtaattc gagtcatatt cacatcttta cgaccatttt cagtcaatgt tagattggta
     4321 gcacgtgtct gtgcattctt ttccaagatt gcttgccatt cttcagattc ttgcgccaat
     4381 cgttcttgtt cagaaaaaga agtcgctttt tgaatatctt caatggttat aaagtatttc
     4441 tcataacctt cagggacctc ttgagtcatt acattgggcg gagaaacatc ttctgtcgtg
     4501 tcctgcgtag ttgtctcttc tgttgtttca cttgtggttt cagtactgat cgcttcttca
     4561 ctactttttg attcttcagt cttcattact tcttcagtag aatttgattc atttatcgtt
     4621 tctgcaaatg ccgttacaga tcctaacatg ggattgagaa atgtaacgaa aattgccaac
     4681 caagttgcca ttcttttaaa catatttttt tcgttccttt cattttattt ttcagtcttc
     4741 taaagtaatt tcttatggac ttcgacaccg aaacattttt atcctcctca aaacaaaaaa
     4801 agactagaac ttataattct agtcttcatc aaccacttac ttttctttta tttttagtag
     4861 aaatctacag tgtgtttttg tgacccgtca ctgtaaataa taacatccac cataaaacct
     4921 ttgttgtacc actgcgaatt tggatcgcta acctctgcat tagcccaatc ataggcttca
     4981 tctacagtgt taaatacctt tccactatta ccaatctctc caactacaat tgttggctct
     5041 tcaggtgtcg ttgtgccacc atttccgcct tgatcgcctc caccaggatt gcttggttct
     5101 tcaggcgttg ttgttcctcc acctgtgctt gggttagaag gagttgtttc tcctccagta
     5161 tttccaccat tacctggttg tgtaggtgtt tgagtagtgc caccattgtt ttctggtgta
     5221 cttggcgttg ttgtaccacc agttgttcct gtttgatctc cagctggtgt tgttggtgtc
     5281 gttgtatttg gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg ttgattcgga
     5341 tcaactttgt ctttctcttg aatcactgtt tgagccacag tttcacctgc gctgttttgt
     5401 acacttgcta cattgccatt aaatactaat ttatttcctg catagattaa atccacattt
     5461 gtaatcttat tcatgtccgc taatttttgg acagtaatat ttgttgcttg actgattgcg
     5521 cttagggtat ctccccagac aattgtgtat tcattccctt taatttctgc tttgatttgt
     5581 tccgccgaac gaggtgtcca aactttgttt tctgctgctt cagcattcgt tcccacagca
     5641 agactaactc ctccgactac gattcctagt gccaatactt tcaacatttt cattgttttt
     5701 cgcctccgat ttgaataaat ttatttacaa aaacatagta ccaaacttat gtctatcagt
     5761 cgatatattt tttgtagatt tttttatttt ttgatatact aactttggta ctaaaataac
     5821 taaaaggaat gtcacacatg caacttaata atttaaatga aatacatgat tgggctcaaa
     5881 ataatttact taatcgtaaa caagctgcca aaatcactgg acaaaactat gatgcttttt
     5941 ctagctcttt acgtagaaat aatatcaaac catttctaga aataggcgac gaacctggtc
     6001 cttccatcat tcgcttatat ctaagagaag atattgaaaa atacgcaaaa cagctcgaag
     6061 aaaaaagaca gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt aaaacaataa
     6121 taaacacttt tacaacggag gttctatgaa taagaaatac attgtttcag ttggttcaat
     6181 cgtactgatt gtactcgcta ttattgcttt ttctgtatcc aacaaaacag aaacacagcc
     6241 acaaaatacg gttcaatcga ctactacaag tcaatctagc gatcattccg tctatccttc
     6301 actaaatagt ttaaaagaaa cgcatggaca agatttaatt gatcttggtt cttctcaaat
     6361 tactactgtc ttatttgaaa agaaaaatat tgcgtttatt actcctaaag aaggaactga
     6421 ccttgaattt tctatcaaag taacaaatac caaagaaaat ttattctcca ttcctgacat
     6481 tcaatatacc cctaacaagt taaagattgg ggatacgttt ggtttggcta aagtaaatga
     6541 acaatacttt gcttactcac tgaaataagt ttctaatagc aagaggaaaa accctcttgt
     6601 tatttttatc acataatcat tttataaaaa cgcttcgaaa atatcaacat ttttgaagaa
     6661 actatttttc taatttctca atttcttcca agggattttc gctaatgacc catacaggca
     6721 cagattgctc tcgaggctcc ccttcttcta accaccaacc tgcataatga acttcttcct
     6781 cctcagcatt accatgatac caaccaacaa aggattcatc tacaacgaat gaaatttctt
     6841 cttctggatc aaataattca tttttccatg cttcaattgc ttgttctttg gtgtactttt
     6901 gtttgttaaa tacaccacaa atttcgtcat ctacataaaa aatttcaaaa tcaaaattac
     6961 tcgttccttt tactggcatc ttaacttttc ctcctttgac tcacttcaat agatatacca
     7021 ctcatgttca ttccattgtt tactagttaa ttggagtttt atcttagggc tttgtgcact
     7081 ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat ttcttacatt
     7141 cggtactaaa tgtttttcct gcatttcttc atgaaaaaca tataattctg cttcattttt
     7201 caaccaattc attattcatt ccctcattca cttcttcata ccgccattca gatagtgaga
     7261 tttctttttg tttctccact tgtcctataa aaatcgctgt attcaacaag ctgatcacac
     7321 ttaataataa agcaatgaaa ctaatggtta cacttactct ttcacatttt gtcattttgt
     7381 ttccttcttt actcgtttgt tatttcttgt aataggtcta ataactcctt caccgaaatt
     7441 tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac acattaccag accttcaact
     7501 gatatatcac tgcgacaata aaattctgga gaaacactga tttctgattc tttcttacaa
     7561 ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt cttccacaac tatgttttgg
     7621 gattctacca agaaagggag tgcctgttct aatgcttctt ctggtgtttg agcagataga
     7681 ataattgctt cttgttcttt tatttcattg ttctcaaata ctttataact ttttttcatt
     7741 gttagttcct ctttcattcc tctctcaatt agctaagccg ttactggtac gccattctct
     7801 aaatttagta tcgctaatcc ttaatgctcg tcgaatatca atcattcgat aacctattgc
     7861 acgaagttct ttatatcttt tagcagttag cgtctctaac gtcatttcag gcaatgggtt
     7921 cgaatcaaaa atagaattcg aaccgattat ttgggccaat tgttgtactt gtaacagctc
     7981 ttctgaactt aattcagtga ttgctcgatt gcctatcatt gcatacattt tctgtcgaat
     8041 atacttcttt tcttgtttag acaacatttc tatccactcc tattttcatt atcatttgtt
     8101 acctccactt cagtaaatcc acaaataaat gaattcatat tccaagtacc attcccttca
     8161 actacaactt ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt taccaatatg
     8221 tttttcacta ctactaattt gtttgtatac cgtggaattg ctgttccgat atacttcact
     8281 ttatttccta cgctaattgt catttcctca ctcctattct tttttatcca aaactttttc
     8341 ctgtcctatt cctgccaacg aatatacttc actgcatgct taaacaaaac aaaatgttgc
     8401 cctgttttat ttccgtcttt tacttgttcc acatacagtt catatttggt cacacctttt
     8461 aaaacgccag atactttttt tcatccattg tcacaattag ttttttcatc aatcgattga
     8521 tactgcactg ctcatttttc ttgttcatat agtattcctt tctttttcaa aactagcctc
     8581 ttttgtttac tagttttcat caatcatcaa cgatttaaca gattttttct ttcgcagggt
     8641 ttctagcgtc agcaacccca aactctaaaa aagtgccttt gctcccttga aaaacgagcg
     8701 gacgattgta ctcgtcatca tatgtttttc atactacctg atccacgcaa ataacctttt
     8761 tttgttctgt gccacaccca tccatacgta tcgcttgaga ttcaccatac ttttttaggt
     8821 ggtttatcct tcctaagtcc ctgttagaca cgctgattaa cttagaaagt cctgtttgta
     8881 tccaattttc aaagatctcc caaaagaaat aagtaggttt acgtgtatcg cttctgttca
     8941 aagtacctgt ttgtacttga gtatttctac tcccctcgtt caagggattc cttccaccag
     9001 ctagcttttc ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg tgtccgtgtg
     9061 cttacttatt cttttttcga tagcgattat ccgctcgcta ctcgctattt tatactttgt
     9121 ttgttagctc aaataaaaac taacaaacaa aaaaggctag tttctttccg ttttattttt
     9181 ttaactgtcg ataaatagtt gttagtttaa ttccaagttc taaaaccaat acgcccaaca
     9241 aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc atgtcttccc
     9301 tctttctttt tggtgtcgct gtggtcgttc ttgccgttca atacttcgat acatttttta
     9361 ttcattattt gctcgttttg ttttcctctg gactgctttt cttgcttttt gaatacttta
     9421 tacgcttttc gtgcctgaat taataggtct tgtcggcgtt tcattcgtga gaaaacaata
     9481 tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg caataatttt cccatcaaaa
     9541 aaaatagttt tcacttgaga aaaaaacagt tggaaatctt gttttgttgc taattttttt
     9601 gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac aatccgattc
     9661 gataacttcc aacgttgccg attaaacaat gttacttcac aacaaatatg acgtaagtga
     9721 ttaggggtca ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa attttgggca
     9781 attgtgacag cccataaatc tgcgtttcca ggtcttcata actacaagaa cgcttggctt
     9841 gaagtaatga atattctgct tgatctgata gttttgttgc cattggatct agattcaatt
     9901 ctcttacaca acattcccaa aaattcaata gcgctctaaa ataaaatcct tttcgattct
     9961 gaattgcttt tccttcatga cgagcaattt tttcattttg aatacaccgt tgatacgttt
    10021 tgcttaaatc ttctgcaaat gtttccccta aaattcgata ctcgcttcca tcagataaac
    10081 catgtgtttg attatacttt cgttgccgct tttctataat tcgtttactg tcgtatatca
    10141 tgtttagctg ctctttcgct tcacgccatt cagctgaact gcgaacaatc catgtcaaaa
    10201 catctttcgg aacaaacgta ctttcgctgt aatgagcaat aaactctttt tgccaatcat
    10261 cagccatttc aacaggacta gtcgtatcga tttttgtatc tagattttct gtatctagat
    10321 tgttagaaga attattaaat aattgtgcag aattttcaca tggatttttt tcgtcaaaat
    10381 catcgaaaat cgctaaatta tcaacagatt cctcctcttc ttttccttgt gcagaatcta
    10441 cactggttga gtcaacagga cctaatgaat acacatcatt ttctgtcgta tctaattctc
    10501 ctagatacaa ccgagctttg tttctttttc ctgtttgttg atcaaatccc atatcctcta
    10561 tttctagtag ctcatattct ttcaattctt ttttgattgt attaacagaa cgtctgcaac
    10621 aattcaatgc attggctaat tcttgttccg taaattcaaa ataaacaaaa ccttgactat
    10681 ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata tacgcgattt
    10741 ttgctttatc agacaatgag cgatattttt cacttgtaat aaataccttt ggaaattgat
    10801 agtataattc attgtattta cgagttgcgg taaatctttt tgttgccatc gttattacac
    10861 ctcatcttgt ccatatatta agaaagaatt ttggtgttgg tagacacgaa catcttccat
    10921 tgtagcaatc aattctttta gataaattcg accgaattca attgttagta agtcgtaatc
    10981 ttctaattca tttttgattt cttcaatttc ttcttcagac acattgagta aaatggctaa
    11041 ttcttgattg gtataagaaa tatagacatg tttcttttca tcaatccgtt tttcaattaa
    11101 ggattcttct aagtactcat tcagtaagct ataggcgtat tttgcttgtg cagtcatcgc
    11161 ataatatttt gacgatttta taaagaatag ttttggcata tgaaaagttg cttgtgtcag
    11221 cacatctgtt gcgcgaaatt tgttttttac catatttttt cctcctagaa aaacacgcaa
    11281 aaaaagctct gtataaatcg aattacacag agtttcttct tgttgatatt taatttctaa
    11341 aatacgtttc gggtaaaaac taaacactta ctaatattta gattgtagaa tcttcgcctt
    11401 catttattca ttttaaagag aaaaagatca agtgaaatgt gcccaaaagt ttgattctat
    11461 taatttttaa acaccattca taatatattt cctataatgt aaaaagcgtc gatacttatt
    11521 aaagcatcga cgttattttt atagtaaatt tatcaaaatt tttgccaaca ttttttccga
    11581 tttttttccc tatttctatc tttccttatc tttcttagtt aatattattt gatgttaata
    11641 tatcactact aatattaaaa aacattgatt tatcatagga taaagagtga tagtttaata
    11701 cttaaagaga catattaatt ttatttaaaa attgacatta atgactctta atcagcgggt
    11761 cgcgggttcg agcccctcac ggcccattgg gtgccaaacc cacggaacag gtaaatgttg
    11821 gtgcgaaagc atttcgaaaa catttacgct gttctttttt tatttttcaa aataactagg
    11881 gctttttatt tttaactcaa agtccactgt agccctttgg actttgagtt aaaaaagaag
    11941 cataattatg aacagtaaag tttctaatat aacaataatt cgagaagctt tgcaaaatca
    12001 tgctgccaat
//
LOCUS       DANJJW010000083        11586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000083 DANJJW010000000
VERSION     DANJJW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(181..666)
                     /locus_tag="PWS82_002103"
     CDS             complement(181..666)
                     /locus_tag="PWS82_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL0907199.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    756..1101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1146..1787
                     /locus_tag="PWS82_002104"
     CDS             1146..1787
                     /locus_tag="PWS82_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0907200.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1806..2441
                     /locus_tag="PWS82_002105"
     CDS             1806..2441
                     /locus_tag="PWS82_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0907201.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2459..3274
                     /locus_tag="PWS82_002106"
     CDS             2459..3274
                     /locus_tag="PWS82_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0907202.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3593..5029
                     /locus_tag="PWS82_002107"
     CDS             3593..5029
                     /locus_tag="PWS82_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0907203.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5218..7665
                     /locus_tag="PWS82_002108"
     CDS             5218..7665
                     /locus_tag="PWS82_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0907204.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7895..8962
                     /gene="rlmN"
                     /locus_tag="PWS82_002109"
     CDS             7895..8962
                     /gene="rlmN"
                     /locus_tag="PWS82_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL0907205.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9155..10258)
                     /locus_tag="PWS82_002110"
     CDS             complement(9155..10258)
                     /locus_tag="PWS82_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0907206.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10811..11359
                     /gene="aac(6')"
                     /locus_tag="PWS82_002111"
     CDS             10811..11359
                     /gene="aac(6')"
                     /locus_tag="PWS82_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0907207.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3844 a         1955 c         2500 g         3287 t
ORIGIN      
        1 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
       61 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
      121 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      181 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      241 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      301 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      361 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      421 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      481 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      541 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      601 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      661 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      721 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      781 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      841 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      901 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
      961 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
     1021 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1081 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1141 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1201 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1261 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1321 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1381 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1441 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1501 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1561 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1621 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1681 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1741 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1801 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1861 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1921 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     1981 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     2041 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2101 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2161 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2221 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2281 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2341 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2401 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2461 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2521 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2581 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2641 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2701 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2761 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2821 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2881 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     2941 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     3001 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3061 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3121 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3181 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3241 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3301 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3361 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3421 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3481 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3541 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3601 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3661 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3721 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3781 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3841 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3901 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     3961 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     4021 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4081 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4141 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4201 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4261 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4321 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4381 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4441 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4501 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4561 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4621 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4681 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4741 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4801 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4861 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4921 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     4981 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     5041 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     5101 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     5161 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     5221 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     5281 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     5341 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     5401 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     5461 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     5521 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     5581 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     5641 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     5701 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     5761 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     5821 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     5881 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     5941 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     6001 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     6061 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     6121 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     6181 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     6241 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     6301 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     6361 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     6421 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     6481 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     6541 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     6601 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     6661 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     6721 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     6781 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     6841 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     6901 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     6961 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     7021 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     7081 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     7141 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     7201 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     7261 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     7321 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     7381 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     7441 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     7501 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     7561 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     7621 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     7681 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     7741 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     7801 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     7861 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     7921 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     7981 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     8041 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     8101 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     8161 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     8221 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     8281 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     8341 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     8401 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     8461 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     8521 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     8581 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     8641 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     8701 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     8761 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     8821 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     8881 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     8941 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     9001 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     9061 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     9121 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     9181 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     9241 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     9301 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     9361 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     9421 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     9481 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     9541 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     9601 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     9661 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     9721 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     9781 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     9841 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     9901 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     9961 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
    10021 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    10081 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    10141 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    10201 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    10261 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    10321 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    10381 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    10441 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
    10501 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
    10561 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
    10621 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
    10681 tttttatttt ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag
    10741 atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg
    10801 gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct
    10861 ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt
    10921 agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt
    10981 aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt
    11041 tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa
    11101 agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg
    11161 aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca
    11221 gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt
    11281 cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc
    11341 tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata
    11401 agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt atttccgaag
    11461 aagctacttt tggaacaccg tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag
    11521 taagaaaaat tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga
    11581 gaaact
//
LOCUS       DANJJW010000084        11573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000084 DANJJW010000000
VERSION     DANJJW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..2115)
                     /locus_tag="PWS82_002112"
     CDS             complement(40..2115)
                     /locus_tag="PWS82_002112"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL0907208.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="PWS82_002113"
     CDS             complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="PWS82_002113"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL0907209.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3418..4230)
                     /gene="recO"
                     /locus_tag="PWS82_002114"
     CDS             complement(3418..4230)
                     /gene="recO"
                     /locus_tag="PWS82_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL0907210.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4373..5272)
                     /gene="era"
                     /locus_tag="PWS82_002115"
     CDS             complement(4373..5272)
                     /gene="era"
                     /locus_tag="PWS82_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL0907211.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5287..5682)
                     /locus_tag="PWS82_002116"
     CDS             complement(5287..5682)
                     /locus_tag="PWS82_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL0907212.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="PWS82_002117"
     CDS             complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="PWS82_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL0907213.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6159..8351)
                     /locus_tag="PWS82_002118"
     CDS             complement(6159..8351)
                     /locus_tag="PWS82_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL0907214.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8375..9346)
                     /locus_tag="PWS82_002119"
     CDS             complement(8375..9346)
                     /locus_tag="PWS82_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL0907215.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10052..11431
                     /gene="brnQ"
                     /locus_tag="PWS82_002120"
     CDS             10052..11431
                     /gene="brnQ"
                     /locus_tag="PWS82_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0907216.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3122 a         2453 c         2114 g         3884 t
ORIGIN      
        1 attttgtgcc tattctctgg tttatttaca tattagaaat tattttgtga tgatttgatc
       61 taaacttgct agcgacaaag ccatggcagc aatttgactt aattgcgtta atcggttctt
      121 acgtactgct ggttcttctg ccatgaccat cgtctgatca aaataatttt cgatcagtgg
      181 gcgaaggctt acaagctgat cataattttc gttgatcgtt tggtcagcaa agcttttttt
      241 cattgcctct actgctttgt gcagttcttt ctccgcttca ttttcgaaca gttcagggtc
      301 tacttctgat tgtttttcaa gctccgcttt tttcgctaag ttcacgacac gtgtcaaagc
      361 ttccatcgat ggtttgaagt cttcatctgc aagatgctgc ttgaacaatt gtgctgctgc
      421 aaatactttt gtcagatctt tttgttctgc attcaaaaca gcttcgatga catcatgacg
      481 aatattcttc gttgtcaata attggcgcag acggcctttg ataaagtcga tcacctgttg
      541 ttgcccttct gataaaccaa tgccgaaacg atccgtatct ttactgataa catcactaat
      601 cgtttcttgc agaacagaca aaggaaaggc ccattttttc gattcgatga tccgaacgac
      661 accgtatgct tggcgacgta aggcataagg atcgtttgac ccgcttggaa tcatcccaac
      721 tgcaaagaaa gaaaaaatgc tatctaattt atctgcaatc gctaaaacag aacctactgt
      781 agactgaggt agctctcctt cactggatat aggcatgtaa tgctcacgaa tcgctgttgc
      841 tacagcaggt ttctcgcctt tcagcagagc atatttttcg cccatgatcc cttgtagttc
      901 aggaaattcc cctaccatgt tggttactag atcaaattta tagatttcag cagctcgttg
      961 cagatccatc aattcttcct cagatagtcc tacttgttcg ccaatgatac gggcaatgac
     1021 ttgtacccgc tgcattttct catagatact accgattttt tcatggaaag taacagtttt
     1081 tagtttttca acgtattggt cgattgaagc tttcttgtct tcattataaa agaattccgc
     1141 atcttctaaa cgagccgtca atactttttg attgcctcga accacatttt ccaattttac
     1201 ccgatcacca ttacgagcag aaataaaatg aggaagcagc ataccttgat cgttccgtac
     1261 ctcgaaataa cgttgatgtt ctttcataga agtgactagt acttcttcag gaacagaaag
     1321 atatttttct tcgaagtcgc cagaaaagac agttggatat tcgactaaat tgttgacttc
     1381 ttctagtaaa tcttcgtcta gatcaataac ccagtgattt ttttcggcta attcatttgc
     1441 ttgtgtaacg atcatttctt gtcttttttg cggtgaagca ataacgaatt gttcattcaa
     1501 tttttcttca tattcttttg catgcgcaaa tgtcacatct tcgcctaaga aacgatgtcc
     1561 tcgagaggct ctgcctgttt tgacatctaa gatctcaaac gggattactt cgtcatctaa
     1621 caatgccacg atccagtgaa tagggcggat atattcaaaa tcgtattctg cccaatgcat
     1681 cgttactggg aaagtgaggc ttttaatgac atctgctaaa cctgtcaata cttctttcgc
     1741 ttctttgccg tgagtgtatt tcgttacata gacatattcc acgcctttta attctctaaa
     1801 agtgatagca tctgtcgtca gaccttgtcc gcggacaaat ccttcagctg ctttcgtcca
     1861 gtttccatct gcgtccaaag caattttttt ggctggtcct ttgacttctt cttcactgtc
     1921 tgcttgacgt tctggaatgt ttgttacttt gattgctaag cggcgcggtg tagaaaacgt
     1981 ttcgatcgta tcataagtca attggttttc atctaaaaat tttgctgttt tttcttctaa
     2041 ttgtttcatg cttggtgtga caacatgagc aggcatttct tccagcccga tttcaagtaa
     2101 taagtctttt gccatcttat tcagcctcct tcatgttttc tggatttgct gtgtttttat
     2161 tcaataaagg gaatcctaat ttttcgcgtt ctgccacaaa gattttggct acagcacgag
     2221 ccatgttgcg gatacgggaa agataacctg ctcgttctgt tacggatact gcaccgcgag
     2281 catctaataa attgaatgta tggctgcatt tcaaaatata atcatacgct ggatgaacaa
     2341 gattttcatc gatacagcgt ttagcttctt tttcaaaacg gtcaaagttg cttagtagca
     2401 agtcttggtc acttacttca aaagaatatt tggaatgttc atattctggt tgtttgaaga
     2461 tctcgccata tttcacacca tcagcccatt ccagatcata gacactttca acttcttgaa
     2521 tatatgaagc aagacgttct agtccataag tgatctcaga agtaactggt ttacaaggta
     2581 gtcctccgac ttgctggaag tacgtgaatt gtgtgatctc catcccatca agccatactt
     2641 cccaacccac accggcacac cccatcgatg ggttctccca gttgtcttca acaaaacgaa
     2701 tatcgtgttc taatggatca attcctaaaa gacgcaagct ttctaaataa agctcttgga
     2761 tattttcagg tgaaggtttc attacgactt gaaattgatg atgctgatac aaacggtttg
     2821 ggttttctcc ataacgcccg tcagctggtc ggcgggatgg ctctacataa gcagcattcc
     2881 atggttccgg tccgattgcc cgtaagaatg tatatgggct catcgtacct gctccttttt
     2941 ctgtgtcata agcttgcatc agcatgcagc cgtttgatga ccaaaatttt tgtaatgtta
     3001 aaatcatatc ttggacggtc aattttttac tcatctgttt tgcccctttc aatttttaag
     3061 cgttgctgtc aataagaata caggaaagaa aacgacaaaa agtccctatg tctgattaca
     3121 gacataggga cgaataattt cgcggttcca ccctacttcc gaataaagat tcggcagctt
     3181 catttatcgt actcgcaagt gcctcttcaa aaatcgtttg ggtccggctt ccactctccc
     3241 gaactcgcta aaccacgtgt tttttactga tcttggtcaa tgtacgtatt cattttgcag
     3301 ttttcttgtt tatataatag atcctctcag tgaacgatct atttaatcta cggttaataa
     3361 tagacggaag tccctgtatt tgtcaaatag ttttgatttt tttgttgtat ctttcattta
     3421 ttcttctttt tcaggtatct ctttttcagg catatttttc tctggcgtct ttaatacgtg
     3481 tgcccaagac ttcatttgat caatgaactt tttgcttttt agatgaatac cgacatattc
     3541 atcatacaac tggtcaatga ggtcacggat tgcctgcttc gtttcttctt tcaagtggat
     3601 tccactgatc ttatcataag aaatagccga aaacattcga acgaaataaa cagcgcgagg
     3661 atctgcatga taacggtgtt cgtccattgg ccagtgtttc tcgcatatca caccgccaga
     3721 tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacacagt gcgtccaaat
     3781 tggcgcgatt ccgaagcgtt gcagtatttg aacttcaaag atattcgtga tgatttctgc
     3841 atcatctcct tgatccatcc gctgcagagc ttgttgcgta aattggaata aatgcgggtc
     3901 atattgatga tcttcgattg ctgcatctac tagatttagc aaataggtac cataaccgct
     3961 caaaaagata tcttcttgga tgttccgaaa cgcatggacg tctttgacac tgcttaaaaa
     4021 cgacaaacct tcacttcgaa aattaccgat atagactgct tcggtaaagg ggagcaaagc
     4081 cggagctaat ggctgatttt gccgatggat tcctttgata taaaacatca ttttgccaga
     4141 agattccgta aagattttga ctaatttatc tttttctttg taattacggc tagataaaat
     4201 caagcctttc gattctgcaa gctggctcat attctccctc ctaaaaaata aatctggaaa
     4261 agcagattct gccgatccag attatttatg tttcgatctt ccgccgtttt tctcaacgtg
     4321 cggattttta tccagtttat ttctatactt aacctataag aagttcagaa tatcagtatt
     4381 cgtcttttcg gtagccaaag tcttgcagat agactttttt gtctcgccag tctttttgga
     4441 ctttgaccca aagttctaga aatactttgt ctcctaaaag attttcgata tcctttcttg
     4501 ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatt cctttttggc
     4561 tatctctttc gacgatgatc gtggcctgga tatggatttt atcattctcg tttcgcttca
     4621 tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa aacaagactt
     4681 tctcacggat caattcagaa acgataaagt attcaggatg atcagtcact tgatcatctg
     4741 gaaaatattg tgggccctct ggcatttcac tgactaatac atccattaag cgttcgacat
     4801 tgtttccttc tgtagcagag atcggaatca cttgtgcaaa aggcatctgc gtcgaataat
     4861 cttcgatgat tcccagcaac tcgtctggat ggatcttatc gattttattg ataatcagat
     4921 aaactggaga atgcacgttt tttagacgtt caataataaa atcgtctccc cggccgcgtt
     4981 tttgatcagc actgatcata aataagactg catccacttc tttcaacgca ctgtaagctg
     5041 cttctaccat aaaatctcct agacgatgct ttggtttatg gattcctggt gtatcgataa
     5101 aaatcagctg agcttctggt gtagtatata ctccttggat tttatttcta gttgtctgtg
     5161 ccttgtcact cataatggca attttttgac ccacgattcg gttcaataat gtggattttc
     5221 ctacattcgg gcggccgacg attgcgacaa accctgattt atgttctggc atatacgttc
     5281 tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca acccaatgat
     5341 gacagcaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga tttttttccc
     5401 aatcggatga aaatgaaaat ccgtaaacat gtccactaca ttttcaaaaa cagtattgat
     5461 gatctctact atccaaacga gaaaaacagc taaaagcagc cataaccact ctatccgatc
     5521 aagctggaaa atcacgccca taaataaagc aaggataccg aacgcaacat gtttcctcat
     5581 attgcgttct tcctcaaaaa cggttttgac tccttggaca gcaaattcca cagacatcag
     5641 gaaatgtttg tttttctcta cgtttttatc ttttgagtcc ataggcatcc aagatctctt
     5701 tctgtaaacc aaacatttct ttctcatctt ctggtgtcat atggtcatac ccgttgatat
     5761 gcaagaatcc atgtaccgct aaaaagccaa gttcccgatc atatgaatgg ccgtaagctt
     5821 ctgcttgttc agcagcacgt tcaatagaaa tcatgatgtc tcctagtaca cgaggcaatg
     5881 gattctctcc atcttcaaaa atcactggga tttcgtcttc gccttcttct tccagagcaa
     5941 aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt acttggattg
     6001 ccgcattgtc catgaaagta acagacattt ctgtttcctc tggcagcttt aaataagatg
     6061 ctgcaaattg cagcaagtca tcgatttctt tcattttctc ttctgagact tggtttgttt
     6121 catccataaa ggtaatatcc attatacatc gcttctttct attcttcttg ttcttccttt
     6181 atcttctcaa cttctgtttt cattttagga tatttgatcc gagaatggaa ggtgctactt
     6241 aaaccgctga tcaatgattt ttccactttt cggatttctt tcaaggtcag tccactatcg
     6301 tctaattgac catcggaaat acgctcttcg ataagattat ggacaaaact ttcgattttt
     6361 tcgctcgtcg gatgatccat tgcccgtact gcagcctcac aactgtcagc aatattgacg
     6421 atccctgctt cacgactttg cggttttggt ccaggataac ggaattgctc ttctgttact
     6481 tccggattgc gttctttcgc tttgatgtag aaataacgca ttagcgtggt tccatgatgc
     6541 tgtttgcaaa tatcgatcac catttgcggc atattataat cttccaatat tttggctcca
     6601 tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa attgtgcggg
     6661 ttttccgctc ctgaaggcaa gttctcaacg aaaaagctag catgtttgat cttgccgata
     6721 tcatgataat agcaagctac tcgagttagc aaagaacgtc cgccgatttc tgctacagcg
     6781 ttagcactta gattagctac catcatacta tgatgataag tacctggcgc ttcttccagc
     6841 aactgtttca ataaaggatg atttggattg ctgagttcat ttaatacaat tacactgtca
     6901 tcattgacca tcaattcaat atatgggtgt agaccgatcg tcagcaagaa tgagaagagt
     6961 gcaccagtga acccgcagac aagcattaac caagtcgttg catcaccaaa gctcattcct
     7021 tgatagacga tcaaaatgac atccatcatc acaggaaaaa taatgaccca catggcagca
     7081 gataatcctt gttctgagat ccgtttgcgt ctgacaatcg ttgccatgag cccagagaaa
     7141 acataagaca tcaagataat cgttagaaag ttcgtgccaa tcgaatcata aaaaatgaaa
     7201 agtgcagata ctgcttggaa aataccagcc atgatccctg ctcttcggtt caagaaaaag
     7261 cttaaaacaa gtggtgcaaa agcagcggga taaaacagcg ggatataagt agcttgttcc
     7321 gtttggaata attggaaaaa tttcatcagc actatgctga ctgacatagc tgtcacatag
     7381 aaaagcacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt ataaatcagt
     7441 acgccgattt gaagcaaaac agctaaaacc atggcaatta gcggaaaaat cgaagttgtt
     7501 tgattagtca gtcctagtaa ttccaatttt ttcatagctg aagcatcgat ttgattacct
     7561 tcacgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc tcgtgcggat
     7621 tgtttcaatt cttctgtctt tttctcattc aatacgtcat tcactacgat accttgatcc
     7681 actaagtagc gaatcatttg ttgttgtgct ggtgtgacat tcaaatattg gatttgctct
     7741 tctgcttctt gtttaaaagc ttccaattca ctttcacgaa cttgtttgct catctgttcg
     7801 tcaatgagct tcagactctc atccctcaca gtatggatct cagtttcagt catttcaaat
     7861 atcgtcgaat aaaaattgtt cggtagtttt tgataaaacg caacattttc tgcattcaca
     7921 ttttcaaaat ttttcttcaa cgcggcgatc cgctcttctg tagtaggctt tgggatattt
     7981 tcatcttcct ttgccttatc aatcttttcg tcataagatt tgttgatggc atcattcgtt
     8041 ttatcgatca attcaaatag ttgcttgatc cgattgttct ggtcatctgc aagatctttt
     8101 tggtaagtgt attctggagt gactgcttct gctgccagtt ttcttttttg ttcggtttct
     8161 tccgtgttct cgatcgtttt gttcgcacgg atactttctt ctgctacttg gccttctctg
     8221 aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcagataat gataaaaaac
     8281 aagaacagca ggaaaggcaa tgctgccttt ccaagttttt cttgtagttt ctgcagtgtt
     8341 ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatggaga tctgcctttt
     8401 cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt gcttcaaaat
     8461 taacaaaatc gattttcttg atttgcttta atgttcgttc tgcatgaaca agaccacttg
     8521 ttgtccctcg aggcagatca atctgactcg tatctccatt gacaatcatt tttgattgat
     8581 aaccaagacg tgtcaggaac attttcatct gagcaactgt cgtgttctgt gcttcatcca
     8641 aaataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaagga gcaatttcga
     8701 tcacgcctcg ttccatcaat cggttcgtat gatctaatcc aaaaatttgg tataaagcat
     8761 cataaacagg acgcagataa ggatctactt tttcttttag atccccggga agaaagccta
     8821 gattttcccc tgcttccaca gcaggccgcg tcaaaatgat tttttgcact tcaccttttt
     8881 tcaaggcagc gattgctaat actactgcaa gaaaagtttt tcctgtcccg gctggtccta
     8941 cgccaaatac gatatcatgt ttggctaccg attcgacata tttcttttgg ccgctgtttt
     9001 ttacccgtat tggttttcca ttacggtctt tgacgatctc ttcctcatac atttcaacaa
     9061 aataatctaa tgtgcctttt tgccccattt tcagtgccgt cacgacatct ggtgtactta
     9121 catggattcc tcgtttgatc aattcctgca atgtccgaat gactgaggca gccaaggaaa
     9181 tctgggattg ttcgccaatg atctgaatga tttctccacg tgtatggatg gttgtttctg
     9241 ttgtttcttc aatcagtttt atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat
     9301 cagcagccgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat agacatcccc
     9361 tttttttcga ttcattcttt cctattttac catactcaga agaaactata tagcaaataa
     9421 gaaaaggttc taaaagagtt gcttaaccat aagtaccaag aaacgttaaa aaatagctgc
     9481 ttccattctc taaaggtctc gtttgaataa aaaaagctga tcatgtaagc tatgcataag
     9541 atagaggaca agtattccgt ctaacttagc aagtattctc tgggtatttt tcttgtttca
     9601 aaaaaagaaa acaaaatatt tgaaatattc ttgcatttca aaaaaaacag gtgtattata
     9661 acgtcaacaa ataaatatac agcaaagcgt cgaggaaaac agtaggctat tcagcgattt
     9721 attcccagtg agtcatgtaa gtgagaaatg acaataaatc gaatagacga acatcatttc
     9781 cgagtcaatg tcgaaaacgt agatcattgt ggctgcctcc attatcaggc tagcggataa
     9841 ctttatcgaa aagcgtccgt gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt
     9901 tctagaatct agagcccttt tcccaagtgg aaaagggctt ttttttatat tttaaggtgg
     9961 tggttttcat ggaatctgat attagtggca tttctagttt ctgcaacgtt tattttgtta
    10021 ctcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt attcatggaa
    10081 gcaattattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag gtaatctgat
    10141 ctttcctgtc cacttaggtc aaatggctgg atcaaactgg atcagcgctg gtgtaggatt
    10201 tgccatctca ggcgctcttt tcccattatt agctatctta gctgtagtag tgacaaaaag
    10261 tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct tgttcttgat
    10321 ccttgttcat ttaacgatcg gaccattctt tggtacaccg cggacagcag caactgcttt
    10381 tgaaatggct gctaaaccct ttttgcctga aaaatatgat tcattaggta tgtttttatt
    10441 ctctgctctt ttctttttac ttgcttattt ccttactgta catccaacac gtttggtcaa
    10501 atatgtcgga aagtatttaa actccttgtt tcttgcatta ttagctatca tttttgtcgt
    10561 ttctttattc caacccatgg ggaatctaaa ccaatctgct gcagattctt atcagacgaa
    10621 cgctttgaca aatggcgttt tagaaggata caacacggtt gatgcagtag ctttacttgc
    10681 tttaagtgtc acttttgtcc atgcagttcg tgatcttggt tttaaagata aaaaagtcac
    10741 agaattaaca gcaaaagctg gtagcctgag cgttgcgtta gaagtcatga tttattttgg
    10801 tctggtcctt ctcggtgcga tgagtttgaa taagatgcct ttatcagaaa atggcggaac
    10861 tgcactttct cagatcacta atttttattt aggtaacttc ggtgctagct tcttaggggt
    10921 aatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt ttgcacaaga
    10981 tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa cgacttttgt
    11041 ctcttttgtc gttgcgaatg ccggattgga taatatcatc caatggtctt taccagtatt
    11101 gatgcttttg tatccattgt cacttgcgtt aatcttgctt tccttaacag caaaattctt
    11161 ccaaaagagt ccatttgtct atcaagtaac gatgttcttt tctgccgttc cagccgtatt
    11221 ggatatgttg gcaaattcgc ctgcattagt gagtcagcaa actgtcgtag caagcttact
    11281 aggattttat catcatcacg ttccttttgc tgcattagga cttggctgga tggtcccaac
    11341 gttacttggt tacgctggca gcttactctt ttattacgcc tatcgcctat cagggtataa
    11401 acaagaagca aatgaactac cagaagaata accaaatcaa acacaattta acataagaga
    11461 tattaaacag gaaaaaaaca gctagaagaa agaatcttat tctttcttct agctgttttt
    11521 tagttctttt tttacgaaca caaaaaactg cggacaatca ctgtccgcag ttt
//
LOCUS       DANJJW010000085        11446 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000085 DANJJW010000000
VERSION     DANJJW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(48..593)
                     /locus_tag="PWS82_002121"
     CDS             complement(48..593)
                     /locus_tag="PWS82_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0907217.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(605..1297)
                     /locus_tag="PWS82_002122"
     CDS             complement(605..1297)
                     /locus_tag="PWS82_002122"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL0907218.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1316..1600)
                     /locus_tag="PWS82_002123"
     CDS             complement(1316..1600)
                     /locus_tag="PWS82_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907219.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1613..2176)
                     /locus_tag="PWS82_002124"
     CDS             complement(1613..2176)
                     /locus_tag="PWS82_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0907220.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2404..3066
                     /locus_tag="PWS82_002125"
     CDS             2404..3066
                     /locus_tag="PWS82_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL0907221.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3190..4386
                     /locus_tag="PWS82_002126"
     CDS             3190..4386
                     /locus_tag="PWS82_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL0907222.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4487..5863)
                     /locus_tag="PWS82_002127"
     CDS             complement(4487..5863)
                     /locus_tag="PWS82_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0907223.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5900..6766)
                     /locus_tag="PWS82_002128"
     CDS             complement(5900..6766)
                     /locus_tag="PWS82_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL0907224.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6855..7880)
                     /locus_tag="PWS82_002129"
     CDS             complement(6855..7880)
                     /locus_tag="PWS82_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0907225.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7898..8572)
                     /locus_tag="PWS82_002130"
     CDS             complement(7898..8572)
                     /locus_tag="PWS82_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0907226.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8756..9649)
                     /locus_tag="PWS82_002131"
     CDS             complement(8756..9649)
                     /locus_tag="PWS82_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0907227.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9723..11420)
                     /locus_tag="PWS82_002132"
     CDS             complement(9723..11420)
                     /locus_tag="PWS82_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0907228.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3388 a         2516 c         1964 g         3578 t
ORIGIN      
        1 tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct
       61 gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt
      121 gccacggcat ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta
      181 tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct
      241 cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa
      301 taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc
      361 cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa
      421 tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc
      481 tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt
      541 ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc
      601 ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt
      661 cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac
      721 gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca
      781 tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt
      841 cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg
      901 ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg
      961 gtaattgtcc tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt
     1021 ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat
     1081 tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga
     1141 cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa
     1201 ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta
     1261 cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata
     1321 taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata agagatttca
     1381 tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg
     1441 gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat
     1501 aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct
     1561 tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc
     1621 ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact
     1681 gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt
     1741 cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa
     1801 ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg
     1861 cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt
     1921 ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa
     1981 attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag
     2041 gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag
     2101 caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt
     2161 gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat
     2221 ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc
     2281 taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata
     2341 agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt
     2401 attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc
     2461 cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg
     2521 tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact
     2581 gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt
     2641 cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag
     2701 acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta
     2761 ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg
     2821 catctgccga atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc
     2881 aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca
     2941 gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta
     3001 gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag
     3061 gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc
     3121 gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag
     3181 gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca
     3241 cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa
     3301 caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta
     3361 ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca
     3421 cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg
     3481 atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg
     3541 atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc
     3601 cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag
     3661 attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta
     3721 aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg
     3781 aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca
     3841 gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat
     3901 tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag
     3961 atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct
     4021 gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc
     4081 gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca
     4141 acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg
     4201 aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg
     4261 atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat
     4321 gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc
     4381 caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg
     4441 ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca
     4501 tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac
     4561 cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg
     4621 agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa
     4681 acatttttct caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga
     4741 tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca
     4801 caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa
     4861 acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca
     4921 tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg
     4981 ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat
     5041 acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta
     5101 tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca
     5161 ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac
     5221 cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta
     5281 caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta
     5341 atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa
     5401 tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt
     5461 cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg
     5521 aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac
     5581 caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt
     5641 ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca
     5701 gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga
     5761 tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac
     5821 tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt
     5881 ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa
     5941 tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa
     6001 ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa
     6061 cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata
     6121 atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga
     6181 agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat
     6241 tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc
     6301 taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt
     6361 gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat
     6421 aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta
     6481 attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga
     6541 actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc
     6601 aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga
     6661 tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa
     6721 tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt
     6781 tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg
     6841 tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc
     6901 attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc
     6961 agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt
     7021 ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct
     7081 gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt
     7141 ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc
     7201 catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt
     7261 tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat
     7321 tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct
     7381 acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata
     7441 tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg
     7501 acccatgagt caatataatc tttttggtct tgttgattac tgattgctga ttgcattact
     7561 tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc
     7621 ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc
     7681 caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca
     7741 atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc
     7801 acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc
     7861 aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc
     7921 ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat
     7981 tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca
     8041 gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat
     8101 attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat
     8161 tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac
     8221 ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa
     8281 aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga
     8341 taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca
     8401 aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga
     8461 acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca
     8521 tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc
     8581 cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt
     8641 acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc
     8701 gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc
     8761 tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa
     8821 gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt
     8881 ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta
     8941 gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct
     9001 tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt
     9061 cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt
     9121 caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg
     9181 tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca
     9241 aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg
     9301 ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt
     9361 gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct
     9421 gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa
     9481 gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta
     9541 aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta
     9601 ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct
     9661 tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt
     9721 gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa
     9781 cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg
     9841 cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct
     9901 tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt
     9961 acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga
    10021 acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac
    10081 tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt
    10141 gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg
    10201 cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct
    10261 actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta
    10321 tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct
    10381 cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct
    10441 gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt
    10501 gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact
    10561 gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa
    10621 tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga
    10681 tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg
    10741 tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa
    10801 tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata
    10861 tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga aatatgagct
    10921 ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc
    10981 acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt
    11041 gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct
    11101 tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg
    11161 taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca
    11221 aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt
    11281 ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt
    11341 tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg
    11401 acaatcattt ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DANJJW010000086        11276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000086 DANJJW010000000
VERSION     DANJJW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(294..2303)
                     /gene="metG"
                     /locus_tag="PWS82_002133"
     CDS             complement(294..2303)
                     /gene="metG"
                     /locus_tag="PWS82_002133"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL0907229.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2571..2849
                     /locus_tag="PWS82_002134"
     CDS             2571..2849
                     /locus_tag="PWS82_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907230.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2946..3767
                     /locus_tag="PWS82_002135"
     CDS             2946..3767
                     /locus_tag="PWS82_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0907231.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4052..5062)
                     /locus_tag="PWS82_002136"
     CDS             complement(4052..5062)
                     /locus_tag="PWS82_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0907232.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5074..6027)
                     /locus_tag="PWS82_002137"
     CDS             complement(5074..6027)
                     /locus_tag="PWS82_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0907233.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6017..7087)
                     /locus_tag="PWS82_002138"
     CDS             complement(6017..7087)
                     /locus_tag="PWS82_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907234.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7093..8136)
                     /locus_tag="PWS82_002139"
     CDS             complement(7093..8136)
                     /locus_tag="PWS82_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907235.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8137..9078)
                     /locus_tag="PWS82_002140"
     CDS             complement(8137..9078)
                     /locus_tag="PWS82_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907236.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9162..9509
                     /locus_tag="PWS82_002141"
     CDS             9162..9509
                     /locus_tag="PWS82_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL0907237.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9579..11240)
                     /locus_tag="PWS82_002142"
     CDS             complement(9579..11240)
                     /locus_tag="PWS82_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0907238.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3170 a         2300 c         2057 g         3749 t
ORIGIN      
        1 aactttatga atgtaaggac aataaaaaag agtaccagat gaatcaatta aaatctgccc
       61 gaccctttat cattatgctt tttttctgtt tttacgattc ctattagtat tattctaaat
      121 taacaaaaat cagcgaagct cgtacttctt tttctaggat ttattttgga tagttctctg
      181 tttctgtatt atatgattgg cttcaagtaa taagaaggaa tttacgaaaa ttgcttctca
      241 aatttcggaa aaagggggta tgacacaact agtgtcatac cccctttgaa tttctatgcg
      301 atttctgagc catttggcat ggattttggt gcttcgatta cttgtaaaga gccatctggt
      361 gcttctgcag aaaggatcat tccttgactg atttgtccac gcatctttcg tggtttcaga
      421 tttgctacaa tgacgacttt tttcccgatc aattcgcttg gatcaggata aaattcagcg
      481 attcctgaca agatttgacg gtcttggctg tctccagcat ctagtcggaa ttgcagcaac
      541 ttatctgccc cttctacttt ttgacagttg atgacttctg ctacttttag ttctactttt
      601 tcaaatacat caaatttgat ttgtttttct ttcgttgaaa caagttctgt ttcttctgga
      661 tcccatttga ctgtatcttc gtttgtttga gtaccttcag acattttttc ttgaataaaa
      721 gcaacttctt cttccatatc taaacgtggg aagattggtg taccttttgc aactactttt
      781 gttcctgaag gaaattcgcc aaagtgtaat ccttcaagat tcattgtttc agagtccaat
      841 cctagttgat tgaagatctt ctctggcgtt tctgtcatga ttggctgaag taatatcgct
      901 gcgatacgca aagattcagc taaatggatc atgacactat tcaattcagc tttcttttct
      961 tcgtcttttg ctaataccca tggttctgtt tcatcaatgt atttgtttgc acgagaaatc
     1021 agcacccaga tttcagccaa tgctgtattg aattccattt tttccattgc atcatggtaa
     1081 cgtccgacaa cgttagcagc cgtcgtagac agttcactat caaatggagt gacaagtgag
     1141 gcatagtcag gaacgagtcc atcacaatat ttgttgatca tagcaatcgt tcgattcaat
     1201 aagttcccta gatcatttgc cagatcataa ttgacacggg aaacaaaatc ttctggcgta
     1261 aagacaccat ctgacccaaa cggaacagca cgaagcaaat aataacggag tgcatctaaa
     1321 ccataacgtt ccacaagcat ttcaggataa acgacatttc cttttgattt agacattttt
     1381 ccgtctttca tcaatagcca gccatgccca aatacttttt taggtagagg caaatcaagg
     1441 gccatcagca tgattggcca atagatcgta tgaaagcgaa cgatttcttt accgaccatc
     1501 tgaacatctg caggccagta tttttcgaat agactttcat cttcggagcc atatcctagt
     1561 gcagtaatat aattggataa tgcatcgatc catacataca ccacatgttt cggatcactc
     1621 ttgactggta ctccccaagt aaaagttgtt cgtgaaactg caagatcctc taatcctggt
     1681 ttgataaagt tgttgatcat ttcgttttta cgagattctg gctggatgaa ttcgggatgt
     1741 tcttcatagt attccaatag acggtctgcg tatttgctca tgcggaagaa gtatgattct
     1801 tcttttacca attctacttc gtgtccactt ggcgctttac caccgatcac gttaccttcg
     1861 tcatctttgt acacttctgc aagttgtgtt tctgtaaaga attcttcgtc agaaacagag
     1921 taccagcctt catattcacc taagtaaata tctccctgtt ctaataaacg atcaaatatt
     1981 ttttgaacag ctgctttatg gtaatcatct gtcgtgcgaa tgaatttatc ataactgata
     2041 tcaagcgttt tccataattt cttgacatca gccgccattt tatctacata ttcttgcggt
     2101 tttacaccta gttcttctgc ttttgtttcg attttttgac catgttcatc cactccagtg
     2161 agataaaaca catcaaagcc catcaaccgt ttataacgag caattgcgtc acatgcaatc
     2221 gtagtatacg agttgccgat atgaagtttg ccgcttggat aatagattgg cgtagtgata
     2281 taaaaagttt ccttttctgc cacggggctc ttcctccttt atcgcatgat gcgaattcgt
     2341 ttccttgcta gtataccata aaaaaagcat gctgttagct ttttttcaaa atgaaaaaga
     2401 ctggataaac gacttctatc tacgttcaat ctatccttta aaaggatgaa taggttgaat
     2461 aaatgcgggt ttttgttttg tttcatatct actaaaaaac ggctttttat ttctcccctc
     2521 acacgtggta taataaagac tatctatctt agtaagggac tggaacaatc atgcagaaga
     2581 aacatctttt ttttacactt agtattgctt ttctttcatt agcacattta atattcagct
     2641 atttttatat tcgtatgtat ggttatttta acctccatgg gcatttgaac agttttatga
     2701 cagcagcttg gattttgcgt tttatcattg acgtatatat cgttatttgt ggcttctttg
     2761 ccattcgtga agagcggtat aaagtattgc cgttttatct gcttttcttt ttattcaatc
     2821 tcatacttcc atttattttt catatttaaa tgataaccta gatttttcaa gtaaatcctt
     2881 ggtttttttt cagagtctgc tacaataagg taaaaagtac ttttactgtc aatggaggtg
     2941 gtcagatgaa ccgttttgct tctaaagaag aagaaaaatt atattatgaa cataaagcaa
     3001 gtgaagaaga atttcttaac tggtatcaaa aacaggaacg tccagaatat gacaagccct
     3061 ctttgactgt cgatatcgta ctgatgtgct ataacaaaga agaagatcag ctgaaagttt
     3121 tgttgatcca aagaaaaggg catccttatc gaaattcatg ggcattgcct ggtggctttg
     3181 tccaaaagga tgagtcaact ggagaaagcg tcttaagaga aacaaaggaa gaaactggtg
     3241 tggttatttc aaaagaaaat attgagcaac tgcatacatt cagtacgcct aatcgagatc
     3301 cgcgaggctg ggtcgtcaca gtcagctact tagcctttat tggagaagaa ccgctaatcg
     3361 ccggagatga tgccaaggaa gtacgctggt tcacactaga acgtcatagt aacaagatcc
     3421 atttatctag cggagaagtg gctatctcgc ttgatttagt cactggagag tcttctggca
     3481 aagatacttt ggcttttgac catagtcaaa tcatcttgaa agcattcaac cgtgtagtca
     3541 ataaaatgga acacgagccg caagttctac aagttttagg taaagatttc acgattacgg
     3601 aagctcgaaa agtatttgct aaattcttag ggatcgatta taaaacgatc gatcattcca
     3661 attttaagaa agctatgctc caatatttcg atgagatcgg tgaacgtcct gtaggaatcg
     3721 gccgtccttc aaaaatctat cagctcaagc cggggcacca tgaataaggt tgtgagcaaa
     3781 agattcagac ctaatcattc aagaaaaaac gcaaaaacaa ctctttgtga gttacgtttt
     3841 ttgtttcaga attttgatgt attcgtaaac tttctgaata aaaaattaac aatagcagaa
     3901 aaagctcgta cgaacttttt ctgctattgt tttattggtt tgtcataatt agtgagcttt
     3961 tcttcaaaag aaaaattcct agaaacaaaa gtcattaaga ctttttatta acaaggctaa
     4021 ccctaacgct cctatcagtt tcattcattt tttatccctt gataaccgaa tgtctgccca
     4081 ttttttctac tacttggctc tttcttacgg aattgctttc ttctgctccg tcggtattga
     4141 tcaaaagaaa ttttttatta taccattggg acttataacg caattttgtt attaatttat
     4201 gccagtcgtg aatagaaaaa tcagcatgtg cctcttcttt tggaaaagat tctacaaata
     4261 agtagtcata ttgtttgacc aatttcccag ataattgttc aagataatct tctcgctggt
     4321 tcttttgttt cagataaaga tccattactt ttctcttttg tttctgataa ttgcttgcct
     4381 gatctaacct gatctttcta tggtgagcgg agcggacttt cacattcaat ttccgctgtg
     4441 catgttgcag cttttcttct gtctggtgaa attttggtaa agttacttct agaggtcgag
     4501 atgtttggat caatgtcttc ggatgatagg cgatcccgat ccatttagct aaagattctt
     4561 tttttactgg cgaaacatca caaagtagtg cgacataaaa ttcttcatta tatcgagcag
     4621 aaatcgtagc ggagcgaatc gatccttcaa ctggccgatg ctgatttatc ttgattagtt
     4681 ctttttgttt tggtaacttt aagtagccat tttccagata gatcgtacgg gtttggttgt
     4741 tggtcgtata agattgccac atactttttt tcgttttaag ctttgggaag ctggcacgtc
     4801 ccttgaaata attgcgaaaa gcccgatcca aattaagctg tgcatttgct aaagcaagac
     4861 tgtctacttc ttttaagaat gggtattctt tttttaatgt agcgggtgtg agcgaaaaat
     4921 tggctactcc tgtttgttgc caacttttct gccgcttttt cagcaaatgg ttataggtaa
     4981 atcgagcaca tccaaaagta cgaatcaatt gctgtttttg ttcttctgtc ggatacatac
     5041 ggaatttaaa agcttttagc atctatgtcc ctcctagtta gcaaccagaa gagattcctt
     5101 ttgtcgtttt agtttttttg cgtaatatgg gacttcctgc tcagttgcat agacaaaatg
     5161 ttctggtgcg atttcacgaa gttgacgtac ttgtccgtca tctggttcag gttgatattt
     5221 gattcttctg cgttttctct catggtctgg atctggtaga ggaatagcag ataacaaact
     5281 ttctgtataa ggatgaacac cgtgattata gatttcatca cttgttccca tctctaataa
     5341 caagccgtta tgcattacac cgattcggtc actaatatgt ttaaccatcg acaaatcatg
     5401 tgcaataaat agataggtca attgatgttc tttttggaga tcttgcaaca aattgaccac
     5461 ttgggcttgg atcgacacat ctagtgcaga aattggttca tcacaaatga taaaacgcgg
     5521 ttttactgcc aatgctcgtg caataccaat acgctggcgt tgcccacctg aaaattcatg
     5581 aggataacga gtaccgtggc ttggattcaa tccaacagtt ctcaatagat catcgacttt
     5641 ttttgcacgc tcttcagatg atttagctag tccattaaca tcaattcctt cggcaatgat
     5701 atctctgact ttcatacgag gattcaacga agcgtatgga tcttgaaaga tcatctgaac
     5761 atcatgccgg aatttcgtca aacctttttt atctttgatc ttcgttatat cttgaccgtc
     5821 gaataatatc tcaccatcag taggttcatt caagcgaatg atcgtacggc cagtagtgga
     5881 ttttccgctt ccagattctc caactaaacc gaatgtttct ccttcataaa tgtgaaagct
     5941 gatgtcattt acagcatgta cttcattctt ttttcctaca ttgaaatact gtttcaatcc
     6001 tttaacttca agtagtttat ttggcatcag caattcctcc ttgaccgtaa gtatgttttt
     6061 gtttctcttt aaagatagcc cagcgacgtt ggatttcttc tggcggtgtc actttgggtg
     6121 cctgcggagc aagtagccaa gttgctgcct tgtgtgtttt tgatagttca aaaaatggtg
     6181 gttcttgttc tgcatcgatt ttcatcgcga attcatttct tggatagaat gcgtctcctt
     6241 ttggcgggtt caataaatcc ggtggagaac caggaatagc atacaatcga tcgttcgcac
     6301 tatctaatgt cggcattgaa ccaagcagtc cccaagtata gggatgttgc gggttgtaga
     6361 aaatttcttc cgatgttcca acttcaacga ttcttcctcc atacatgaca gcgacacgat
     6421 cagcgacatt cgctactaca ccaaggtcat gagtgataaa gataattgat gtatcgattt
     6481 ttgtctgaat ttctttcagc agctccaaaa tctgtgcttg aatagtcaca tcaagcgctg
     6541 ttgtaggttc atcagcaatc aggatttccg gataacatac cagtgcaatc gcgatcacga
     6601 tccgttgacg ctgacctcct gagaactgat gcggataact gttgatacgt ttctcagctt
     6661 cgggtattcc aactaattta agcaattcta atgcagcttt ttgtccctct ttttttgaaa
     6721 ctttattatg ctttctaagg gactcagcga cttgcttccc aatgggcatc gtcggatcta
     6781 aagaggtcat tggatcttgg aaaatcatcg ctatttcttt accacggatt ttttgcatct
     6841 ctctttctga ttttccaacg atatcgttac ctttaaagag gatttgacca gcatctatat
     6901 tggcgttgct tgacagcaag cgcatgatgc ttctggtcgt cactgatttt ccacttccag
     6961 attccccaac gatcgccaat gtttctcctt tattcaaatg aaaattgaca ccgcgaatcg
     7021 ctcgtacctt tccagcatac gtatcaaaag agatttctaa gtctttcact tctaaaattt
     7081 tcgtcatcgt cttcactctt tcattttagg atcaaaggca tcacgcaatc catcagcaag
     7141 taagttaaag ccaatcatga taaccgataa agttactgcc ggaatccata atagataagg
     7201 cagatacagg aatgtcttgt acccttcgct caacatcgtt cctaatgaag ctgacggagg
     7261 cgtaagacct aaaccgataa agcttaaaaa ggcttcgaag aaaatagcag atggaatact
     7321 aaacatcatt tgaataataa tgacactaga gatattcggc aaaatatgtt tgaatgcaat
     7381 tttcattctt gattcaccta atgtcaatcc agctaaaaca tattcttgat cttttaattt
     7441 cattgtttgt gcacgaacga tacgtgccat tggtatccag tttgtgatgg ccatagcagc
     7501 cacgattgaa aaaattcctg gttcaaaaac gaccaacatc aaaatcatga caacgaggtt
     7561 tggaattcct gataagattt ccaaaaaacg ttgcatcacg ttatcgacac gtccgcctaa
     7621 caaaccagaa accaacccgt aagctacacc aatcgttaca tctagtaatg cagcaataaa
     7681 tgcgatcagc aacgagattc tcattcccat aaataagcgg cttaatacat cacgtcccaa
     7741 accgtctgtt cctaaaaaga aatttacgtt tgaagggacg tttgcttgag catatttatc
     7801 aacaagttca ccaccaacca ttgcttttcc attcaatcca tcgatattga ttcccggaat
     7861 acgaggaggc aaattgatat aagccacgtt ttgggcagtt ggatcatgag gagaaacaaa
     7921 gattgtgatc acagagatca aaatgatgac caatagtaaa accattgaaa taacggcagc
     7981 tttgtttttc tttaaacggc gccatgaatc ttggataaaa ttcaaagaag gtgcagtaat
     8041 ctgttcacgt tcttcttcgg tatttttttg taatggaaga aattcatccg cagggatatc
     8101 tgcaactgtt tcataacgct ttggtatcat ttccatttat cctcggctcc cttctgaaac
     8161 acgaattctt ggatcaacta gaccgtacaa tagatccacg atcaaaataa caaaaatcag
     8221 cataaatgag taaaggatcg ttacagccat gatcgtagga taatcatttg ttgtgattga
     8281 cttaacgaat tgttccccga taccaggaat agcaaagata ttttctacca ccagtgatcc
     8341 ggtcatcaat gctacagcta acggtcctaa taaagtcatc aatggaatca aactatttcg
     8401 aagaccatgt ttaaaggcga tttcccaacg gctcaatcct tttgcttttg ctaattccac
     8461 ataatcacta tgcagtactt ccaccatttc cgtacggata aaccgagcag agtcagcaag
     8521 cggcgacatg gccaacgcaa ttgttggtaa gattgtgtac acaaagccat cccattttgc
     8581 gattggcaaa acttgtaatt tgattgcaaa gatatattgt aataaaacag caaatacaaa
     8641 gtttggaatg gaacgaccta atatcgctac gagtgtagaa gatgtatctg cccatgtatt
     8701 ttgcttcatg gcagagatcg ttcctaaaat cgtccctagg actgttccaa agataattgc
     8761 ctgtaaccct aactgcaagg atggtccgat tcttccagct aacaaatgtg cgactggctg
     8821 gtttttaaat tggaaggaaa ttccaaaatc tccttgaagc aaattagaaa gataaatccc
     8881 atattgtacg ataactggtt tatccaaacc gacttgttta tttagcatct cgattgtttc
     8941 aggactcaaa cgttcctgat tcgtatatgg cgtgcccggc agcaattgca tcaagaaaaa
     9001 ggtgattgtt gcaattaacc aaagcgtgat gatcatgaaa aagacacgtt ttaatacata
     9061 tttgatataa ctattcacta atgtttcccc ctagcttttg acttctgaca gatgtttcgc
     9121 tacattatta gataatgaac caaaaataaa ggagcaatca aatgaaaaaa aaatctatcc
     9181 cctacgcagt agcttttcta ctgattctgg ttattttaat aaaaaatctc atcaaccatt
     9241 cctttacact cattcaatta tctaatgatc tattcttatg gtcattgccg tttctgatta
     9301 tcggcggttt tttatgggtc ttttcttcgg gtttcttcga tcattttcag cgatcggtcc
     9361 atcttgcacg aacaaggaac cgaaagaaaa aaccagaatt ttcctcgctc tcgtctgcca
     9421 gctacggtat gtacagcttt tggctgatca ttgccggaat actaattgct ctttctgcta
     9481 ttttcatgct tttttctttg ttaggataag tgaatatgag aacgaggttg cgacatttgc
     9541 cgcagcctcg ttttttctca tgctcacggt attaacgatt attcttcaac gtatgtccat
     9601 ttgtaatccc atgaagcgcc tgccgcatgg tgaacgatac ctttgatttt ttcattcact
     9661 aggtgacctt ctgctttttg gtacaccgga acgacaccca tatcatcaga gatgatcttg
     9721 attgcttttt ggaaatcatc ccaacgggct tgttcatcgc cagcgtctgt tgatgaagct
     9781 gctttaactg cttcgtcata ttctgcattg ctccagcgac cacggttgta tgaattacct
     9841 gtgacaaaca aatcagtaaa gctgcttggg tctgcatagt cagcagccca tccgcctagt
     9901 actgcgtcaa aatcacccga agttgagcgg tcaagacgta cagagaatgg aacaggtgtt
     9961 ggtttgactg ttacgccatc caagttttct tggattgagt tttgaacata ttcgattact
    10021 tttttcgatg aatcatcatc agaagccatc aaatcaaatt ctaaagaatc aacgcccaat
    10081 tcttttttcg ctttttccca atattctttt gctttatctt tgtcataaga aactaatttt
    10141 ccagcttctt ttgcaaagtc ttcgttcgtt gttggacttt ttaccatatc tgatgggatc
    10201 aatcctgtag agacaactga tccgtcacct agtacttgtt ttactaaagc ttcacgatcg
    10261 atagaataag aaatagcttt tcttaggttc acattgttga atggtgaatt cttttcacgt
    10321 tgattgaatt cgatatagct tgtccgcgct tctttaacag aagtataagc tggatcgttt
    10381 gcgttttgct gtgctaactc accagacaag atcacgtcat ctgcttggcc atctttgaat
    10441 aagttcaatg cagtagaaga ttcttttact acactgactt tgatttcaga aagttttact
    10501 gtatctttgt cccagtaatg atcatttttc ttataagtcc attctgtatc tgttcctggt
    10561 ccatcaaaat cttctaatgt gaatggtcca ttataaacag ctttatcact tgcagatgca
    10621 taggcatccc cattttcttc cactacggat tgtttttgtg ggaaaaatga tgggaaagca
    10681 agcagatatt ggaaatatgg tgtttgtttc gtcaatttga tttctaattc atagtcacca
    10741 actgctttga tccctagttc tgatttatct ttctttccag ctgtaatgtc ttctgcattt
    10801 tcaactggcg catacaggta agcatattca gaagctgtag ctgggtctac tgttcgttgc
    10861 cagccataaa cgtaatccgc tgctgtaaca ggatcaccat ttgaccattt tgcgtcttct
    10921 cgtaatttga ttttgtaagt taatccgtct tcactgactt ctgccaactc gctagctcct
    10981 gccggctgtg gtttgctgtc ttcatcaaga cggtaaattc cttcataaac gttattcaat
    11041 gctgtaaaac tgattgtatc tgttgcaact gataagtcag ctgtaggcat ttcttgaggc
    11101 acgactaagt taaatacgcc tcctccatct gctgaaccgc tttttgctga actactgctt
    11161 gtggctgaac tgccgccata acatccagca agtacaaggc cgcataccgc aaccaaacca
    11221 aatgtaaatt tctttttcat tttacgtacc cctttattgg aattttcaga ataatt
//
LOCUS       DANJJW010000087        11117 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000087 DANJJW010000000
VERSION     DANJJW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..617)
                     /locus_tag="PWS82_002143"
     CDS             complement(<1..617)
                     /locus_tag="PWS82_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0907239.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQS"
     gene            735..953
                     /locus_tag="PWS82_002144"
     CDS             735..953
                     /locus_tag="PWS82_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907240.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            1148..1609
                     /locus_tag="PWS82_002145"
     CDS             1148..1609
                     /locus_tag="PWS82_002145"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0907241.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(1802..2041)
                     /locus_tag="PWS82_002146"
     CDS             complement(1802..2041)
                     /locus_tag="PWS82_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907242.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2065..3588)
                     /locus_tag="PWS82_002147"
     CDS             complement(2065..3588)
                     /locus_tag="PWS82_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0907243.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(3606..3728)
                     /locus_tag="PWS82_002148"
     CDS             complement(3606..3728)
                     /locus_tag="PWS82_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0907244.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(3758..4741)
                     /locus_tag="PWS82_002149"
     CDS             complement(3758..4741)
                     /locus_tag="PWS82_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0907245.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(4765..4914)
                     /gene="rpmG"
                     /locus_tag="PWS82_002150"
     CDS             complement(4765..4914)
                     /gene="rpmG"
                     /locus_tag="PWS82_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0907246.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(4935..5312)
                     /locus_tag="PWS82_002151"
     CDS             complement(4935..5312)
                     /locus_tag="PWS82_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907247.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(5344..5523)
                     /gene="rpmF"
                     /locus_tag="PWS82_002152"
     CDS             complement(5344..5523)
                     /gene="rpmF"
                     /locus_tag="PWS82_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0907248.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(5538..5807)
                     /gene="rpsN"
                     /locus_tag="PWS82_002153"
     CDS             complement(5538..5807)
                     /gene="rpsN"
                     /locus_tag="PWS82_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0907249.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(6330..8096)
                     /locus_tag="PWS82_002154"
     CDS             complement(6330..8096)
                     /locus_tag="PWS82_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907250.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(8056..9809)
                     /locus_tag="PWS82_002155"
                     /pseudo
     CDS             complement(8056..9809)
                     /locus_tag="PWS82_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9919..10488)
                     /locus_tag="PWS82_002156"
     CDS             complement(9919..10488)
                     /locus_tag="PWS82_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL0907251.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(10506..>11117)
                     /locus_tag="PWS82_002157"
     CDS             complement(10506..>11117)
                     /locus_tag="PWS82_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0907252.1"
                     /translation="LKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIE
                     NKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPF
                     DLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATH
                     DPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         3496 a         2151 c         1595 g         3875 t
ORIGIN      
        1 ttggactgta acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag
       61 tcactattat ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga
      121 atatcctgtt cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc
      181 gtttcgaaat tatcccaaaa acgttttaca tccactacta taataatggc accaatctct
      241 aatcgttggg tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt
      301 ggaattggtt tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc
      361 actaattgat ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc
      421 ggcatcgaaa caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca
      481 tcaacattta cttcccctat gtcatttaca atgacagcaa tttcctttcc gtgatcaccg
      541 tacagaatat gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta
      601 ataggtattt ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa
      661 cacataaaaa tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta
      721 gaaggaaaat aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct
      781 ttctgcttca cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc
      841 cgtttactat tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc
      901 ggaaaaaaat tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc
      961 gtgagtttgg tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat
     1021 ctcgaatcgt ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg
     1081 tgcaatttgc aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat
     1141 ccgttcattg caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg
     1201 aaagatttgc cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga
     1261 ttttggtcat tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac
     1321 tttgacagac agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc
     1381 cgcttatgtg agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa
     1441 aacgatcact ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt
     1501 gaataatatg cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa
     1561 aatacgaata gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat
     1621 aactcaacga taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat
     1681 tggagaacac cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag
     1741 acataataaa aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca
     1801 attaattggc caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct
     1861 tgataaaaca acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat
     1921 gtaaagttgc agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc
     1981 gataacgccg ataagcatca atattcttgg aatccataga caataatact tttcttacca
     2041 tgtatttcct ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt
     2101 agacaatgaa gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg
     2161 actttctcca ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc
     2221 actaaattga atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg
     2281 aacaccacgg tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta
     2341 ttgatgatgt tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc
     2401 aatatctgtc ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt
     2461 attgattttt gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata
     2521 aacagattgc cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt
     2581 aaagtaccca ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc
     2641 accagcaacc atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata
     2701 atcttcacca ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa
     2761 ttttatccca gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa
     2821 gtaaatataa ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga
     2881 agatggctct tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa
     2941 agctaaatat ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag
     3001 actagtggta tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt
     3061 ctcaaatact tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac
     3121 ttccttaatt gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc
     3181 ttcttctccg tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt
     3241 tccttctact acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga
     3301 ttccatgttt tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt
     3361 agctgagggc tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc
     3421 ttcatcgccg acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc
     3481 taattttcca tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa
     3541 taatcctaga gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc
     3601 cttaattatg ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc
     3661 ctttttctag ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt
     3721 ttctccatta gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt
     3781 tatcaaaact ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg
     3841 tttggtcttt ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg
     3901 caactataaa atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt
     3961 ggttggttaa aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat
     4021 ctgaagttaa gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc
     4081 cattatagtg attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca
     4141 aattataaaa atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata
     4201 aagggttaat acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt
     4261 gttttctaga cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc
     4321 tttcaataag ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc
     4381 ttcctcttag ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac
     4441 ctgtagtttc aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta
     4501 aagaaatcaa tattgctaat aaatcactgc gaagactgca acacatacat ccattattta
     4561 gttgtaacac ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa
     4621 actcattttc aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat
     4681 tgattagcgt tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca
     4741 ttttgtcacc tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa
     4801 ttttggcgaa tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt
     4861 gagatacaga cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct
     4921 tcctccttca atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa
     4981 gttaactcgc cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc
     5041 caataaccat cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt
     5101 cttaaaaaat catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt
     5161 tcaattgcct gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa
     5221 cgataaaaag tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct
     5281 tcttcaacta aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt
     5341 aaactatttt gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac
     5401 atgatggcta cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac
     5461 agtttgatga gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc
     5521 catgatattt cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg
     5581 gcaagttctc taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga
     5641 ccatccgtct gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg
     5701 agagcggtgc gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc
     5761 atcgccattt gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact
     5821 ccttttattt tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt
     5881 accaacaaga gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga
     5941 taactatttg aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat
     6001 gcagtttagg ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc
     6061 gttttaagta gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat
     6121 tcaatctttt atgtttaact ggtttatcgc caatttttta aaactttgat tattccttat
     6181 attataagaa tagacattaa gtgaactaat tcaatcatac tgattctttc gtgtattcaa
     6241 aattagatgc tgtttaataa aattattctt tttgaaaaag aattataact atatcccaaa
     6301 aattgtaggt taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg
     6361 aatttctcag atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac
     6421 aactcattat gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt
     6481 accttttcaa tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa
     6541 cgatttaaac tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca
     6601 tctaaaatta aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc
     6661 tctcctccag aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt
     6721 ttttcaataa agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac
     6781 gcttgactat ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct
     6841 tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac
     6901 ccatcaatta aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata
     6961 ctagttttcc cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta
     7021 aaagagatat gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac
     7081 tcaatgtcaa aagatttcag aggactatct gatccctctt ggatactggt ttccttcata
     7141 gctctaattc gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata
     7201 gaatcgaagg aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata
     7261 gtcacttcac ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca
     7321 tatgagaaaa tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga
     7381 atcctttcac tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta
     7441 aagctattaa tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc
     7501 aaagtatcgt aatatttagt attggctttc gtttgggatt tctttgataa caagattaag
     7561 acaaaactaa ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa
     7621 agcatcactg aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga
     7681 ctcatcgcat gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa
     7741 ttatgttttg aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt
     7801 aaatgcgcac tttctttata agtactatta tataagtttt catactccct atctaacaac
     7861 caatacatga aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta
     7921 ttttctaaaa ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg
     7981 cagtatacaa aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt
     8041 aaagcaaatt tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc
     8101 ataattagtg accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc
     8161 acgttctact attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga
     8221 taatcgatga gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg
     8281 tagttcatac tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt
     8341 tgcatctttt aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata
     8401 aactccttta ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc
     8461 gcaaccggct aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat
     8521 agaaacgtta tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat
     8581 tgtgttaata attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg
     8641 tcccttatcc acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc
     8701 tgattttcct actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa
     8761 aacacactct tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat
     8821 ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag
     8881 tgtttcgact gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa
     8941 agaaacaaaa ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt
     9001 gggagtggta agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc
     9061 aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc
     9121 aaacttagca taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc
     9181 aaatatcttt ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc
     9241 taacgattct tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa
     9301 tacaccaatt actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc
     9361 taaggcaact gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc
     9421 aattgccata tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga
     9481 atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa
     9541 attagtctct aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa
     9601 agctctaaag ttaaccaagc aacagtttga accgcataac tcttagctaa atattcatct
     9661 cctacaataa ttagagactg aagaaatttg taaatactgt aatatccata gactgttaat
     9721 acgacagaaa aatcagaaaa taaagtggct aaccatccgt agtatttcat tttagggaca
     9781 taactaaata attttttgta tactttcatt ttttcctccc ccgcattcaa tttcttctct
     9841 tattcgttta caactgttaa tgctttccgt aatagcgtaa attagagatg tacaaacaga
     9901 taaaaggtaa actacgttct attttttgta tttttttaag acttttcttc caatcaatac
     9961 accaatcata gataaacttg ctacaactaa cactgaaatg attccccata tacctaccgc
    10021 atagtggatc attgcatcca ttgttgcagc agattgtcct ttatctatcc aatcttgacg
    10081 aaaacgatct gtaaagaacc atataggaat ccactgtccg gcagcactgc ctaattgcat
    10141 tagagcccaa gacgcaactt gaggaattgc tcgatcatat ccaaatacag atagaataat
    10201 atcagctaag atcgccatac taatcgaact gataaaccaa ggtaagtacg ccccgcccat
    10261 taacgtaaaa aataacaata aaacaattgt gtaaatagta aaaattcctt ttctagggta
    10321 gttatagcta atataaacaa atatgattcc agataataat ccaaaaatcc cgggggatat
    10381 gctgtgacca aacgccccac caatactact aaccattatt gaaaaaaagt aaattactag
    10441 ggaaataact gtagctaaac caataaaaat ataatctttt gttttcatat atattctcct
    10501 caattctatt tcgttatgtc attaaaaatc tgttctatca aagttgaatc ttctactcta
    10561 agttttatat ctttttttat tccaccattt tttaaataaa taattctatt gcatgtttta
    10621 taaagaaatt ccggatcatg agtagctata actactgcac ccttttcttt taagtcttta
    10681 ataaggtcag caatcctttc catattaata taatccaacc cacttgtagg ttcatcaaat
    10741 agaaataaat tagtttttga caatgaagct atagaaacca ataatctttg tttttctcca
    10801 ccggataaat caaacggtaa ttgatctttc attttataaa gttccatttt ttttaaaatt
    10861 ttttcttgct gatctttact aactatttca ttccccaggg ctaactcgct cctcactgat
    10921 tctgtgaaaa attggtaatc catttcttgc attactaaaa acggcgatga caaatttttt
    10981 ttattttcga ttatctttcc tttggtaggg cgaataatct tcatgagtat tcttagtaag
    11041 gttgttttcc ccacaccgtt tttccctacc aaaccaataa catctccaac tttgacatca
    11101 aaatttatgt cttttag
//
LOCUS       DANJJW010000088        11094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000088 DANJJW010000000
VERSION     DANJJW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            56..1315
                     /gene="lysA"
                     /locus_tag="PWS82_002158"
     CDS             56..1315
                     /gene="lysA"
                     /locus_tag="PWS82_002158"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL0907253.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1302..2102
                     /gene="dapF"
                     /locus_tag="PWS82_002159"
     CDS             1302..2102
                     /gene="dapF"
                     /locus_tag="PWS82_002159"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL0907254.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2105..2986
                     /locus_tag="PWS82_002160"
     CDS             2105..2986
                     /locus_tag="PWS82_002160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL0907255.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2983..3699
                     /locus_tag="PWS82_002161"
     CDS             2983..3699
                     /locus_tag="PWS82_002161"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL0907256.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3705..4400
                     /gene="dapD"
                     /locus_tag="PWS82_002162"
     CDS             3705..4400
                     /gene="dapD"
                     /locus_tag="PWS82_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL0907257.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4416..5417
                     /locus_tag="PWS82_002163"
     CDS             4416..5417
                     /locus_tag="PWS82_002163"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL0907258.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5414..6775
                     /locus_tag="PWS82_002164"
     CDS             5414..6775
                     /locus_tag="PWS82_002164"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL0907259.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6735..7886
                     /locus_tag="PWS82_002165"
     CDS             6735..7886
                     /locus_tag="PWS82_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0907260.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7861..9030
                     /locus_tag="PWS82_002166"
     CDS             7861..9030
                     /locus_tag="PWS82_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0907261.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9205..10584
                     /locus_tag="PWS82_002167"
     CDS             9205..10584
                     /locus_tag="PWS82_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0907262.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10865..11094
                     /locus_tag="PWS82_002168"
                     /pseudo
     CDS             10865..11094
                     /locus_tag="PWS82_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3688 a         1942 c         2494 g         2970 t
ORIGIN      
        1 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
       61 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
      121 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
      181 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
      241 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
      301 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
      361 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
      421 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
      481 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
      541 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
      601 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
      661 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
      721 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
      781 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
      841 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
      901 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
      961 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     1021 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     1081 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     1141 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     1201 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     1261 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     1321 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     1381 tagccgcttc tttatgcaca aaagaaaaat atgatacaga cggattgatc gccgtaaaaa
     1441 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     1501 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     1561 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     1621 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     1681 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     1741 tatttttagg aacgattcat acagttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     1801 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     1861 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     1921 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     1981 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     2041 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     2101 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     2161 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     2221 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     2281 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     2341 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     2401 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     2461 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     2521 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     2581 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     2641 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     2701 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     2761 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     2821 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     2881 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     2941 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     3001 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     3061 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     3121 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     3181 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     3241 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     3301 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     3361 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     3421 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     3481 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     3541 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     3601 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     3661 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     3721 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     3781 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     3841 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     3901 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     3961 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     4021 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     4081 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     4141 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     4201 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     4261 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     4321 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     4381 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     4441 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     4501 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     4561 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     4621 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     4681 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     4741 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     4801 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     4861 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     4921 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     4981 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
     5041 atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
     5101 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
     5161 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
     5221 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
     5281 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
     5341 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
     5401 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
     5461 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
     5521 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
     5581 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
     5641 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
     5701 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
     5761 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
     5821 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
     5881 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
     5941 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
     6001 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
     6061 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
     6121 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
     6181 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
     6241 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
     6301 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
     6361 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
     6421 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
     6481 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
     6541 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
     6601 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
     6661 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
     6721 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
     6781 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcggggccg
     6841 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
     6901 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
     6961 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
     7021 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
     7081 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
     7141 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
     7201 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
     7261 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
     7321 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
     7381 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgcaatgtt ttagctggag
     7441 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
     7501 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
     7561 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
     7621 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
     7681 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
     7741 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
     7801 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
     7861 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
     7921 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
     7981 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
     8041 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
     8101 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
     8161 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
     8221 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
     8281 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
     8341 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
     8401 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
     8461 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
     8521 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
     8581 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
     8641 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
     8701 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
     8761 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
     8821 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
     8881 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
     8941 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
     9001 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
     9061 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
     9121 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
     9181 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
     9241 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
     9301 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
     9361 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
     9421 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
     9481 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
     9541 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
     9601 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
     9661 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
     9721 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
     9781 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
     9841 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
     9901 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
     9961 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    10021 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    10081 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    10141 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    10201 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    10261 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    10321 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    10381 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    10441 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    10501 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    10561 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    10621 tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    10681 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact
    10741 gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt
    10801 cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt
    10861 tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct
    10921 taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa
    10981 ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa
    11041 agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DANJJW010000089        10922 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000089 DANJJW010000000
VERSION     DANJJW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..281)
                     /locus_tag="PWS82_002169"
     CDS             complement(<1..281)
                     /locus_tag="PWS82_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907263.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="PWS82_002170"
     CDS             423..1541
                     /locus_tag="PWS82_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0907264.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="PWS82_002171"
     CDS             1669..3045
                     /locus_tag="PWS82_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0907265.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="PWS82_002172"
     CDS             3446..5026
                     /locus_tag="PWS82_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL0907266.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="PWS82_002173"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="PWS82_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="PWS82_002174"
     CDS             complement(5652..5972)
                     /locus_tag="PWS82_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL0907267.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="PWS82_002175"
     CDS             complement(6120..8354)
                     /locus_tag="PWS82_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0907268.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="PWS82_002176"
     CDS             8833..9099
                     /locus_tag="PWS82_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL0907269.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="PWS82_002177"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="PWS82_002177"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL0907270.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DANJJW010000090        10817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000090 DANJJW010000000
VERSION     DANJJW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..378
                     /locus_tag="PWS82_002178"
     CDS             <1..378
                     /locus_tag="PWS82_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0907271.1"
                     /translation="KKEKLPIDFRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQL
                     FEKHSGRQMTIATSNKSMVLFSTTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPK
                     FGSINLHPGQERISQTIYRFSAQ"
     gene            400..1419
                     /locus_tag="PWS82_002179"
     CDS             400..1419
                     /locus_tag="PWS82_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0907272.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVTPHRLIKRETVVP
                     LENPY"
     gene            complement(1700..3067)
                     /gene="nhaC"
                     /locus_tag="PWS82_002180"
     CDS             complement(1700..3067)
                     /gene="nhaC"
                     /locus_tag="PWS82_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0907273.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(3177..4598)
                     /locus_tag="PWS82_002181"
     CDS             complement(3177..4598)
                     /locus_tag="PWS82_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0907274.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(4841..6718)
                     /gene="tdc"
                     /locus_tag="PWS82_002182"
     CDS             complement(4841..6718)
                     /gene="tdc"
                     /locus_tag="PWS82_002182"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0907275.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(7009..8265)
                     /locus_tag="PWS82_002183"
     CDS             complement(7009..8265)
                     /locus_tag="PWS82_002183"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL0907276.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(8377..8582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8845..10146
                     /locus_tag="PWS82_002184"
     CDS             8845..10146
                     /locus_tag="PWS82_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0907277.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(10215..10814)
                     /locus_tag="PWS82_002185"
     CDS             complement(10215..10814)
                     /locus_tag="PWS82_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0907278.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         3296 a         2259 c         1937 g         3325 t
ORIGIN      
        1 aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca
       61 tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact
      121 tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa
      181 agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag
      241 atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc
      301 cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat
      361 cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat
      421 gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct
      481 agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt
      541 cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta
      601 ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca
      661 atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt
      721 gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt
      781 ctagtgtatt cagatagtca agatcctgtc attgattatt tataccaaaa ccacatccct
      841 ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat
      901 aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc
      961 ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa
     1021 aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag
     1081 gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt
     1141 gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtacggcta tgtcattcca
     1201 gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac
     1261 cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc
     1321 gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcacccctca tcgcttgatc
     1381 aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg
     1441 ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt
     1501 gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
     1561 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     1621 atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc
     1681 agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta
     1741 atattgtaaa gacaggtgca agaatcgaga aaaagacgaa tggcaagtaa tgcaacgcgc
     1801 taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga
     1861 cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat
     1921 caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga
     1981 tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca
     2041 tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc agtaacccac
     2101 ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc
     2161 gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga
     2221 tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag
     2281 ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata
     2341 aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga
     2401 tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa
     2461 aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga
     2521 atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt
     2581 tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa
     2641 gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc
     2701 cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag
     2761 aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa atccatgaag
     2821 caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt
     2881 tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca
     2941 aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta
     3001 ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt
     3061 tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact
     3121 tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta ttttttttat
     3181 ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa gtgattgatg
     3241 atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc atcactcaat
     3301 gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag catgcagctg
     3361 actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc gaattctgga
     3421 tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat acaataaata
     3481 acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat tacaccataa
     3541 agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct agatacgccg
     3601 tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg agctacttgg
     3661 atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac accaataaat
     3721 accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg acagtcaatc
     3781 aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac atttgctaca
     3841 agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa tacgcctttt
     3901 gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat ttcgatccca
     3961 acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc caagtcaggg
     4021 aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc gactttgaag
     4081 gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc tagccataca
     4141 ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt gatgatccag
     4201 tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc tacattacca
     4261 aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc tgggaacatt
     4321 tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc tgtttttacc
     4381 cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga aaccattgat
     4441 atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt ccaaccgact
     4501 gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag accgataaag
     4561 gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct ccttgatttt
     4621 ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca attcagcgaa
     4681 tatttgaggg gattctttga gtctattgct tgaagggttt cttcaaagac aacctcaaaa
     4741 caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat gcgtcagaaa
     4801 cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct tcgcttgcca
     4861 ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg attttttcag
     4921 catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg gcacgcaata
     4981 cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg ttcacaaatt
     5041 gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt aagaactcat
     5101 taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg ttcaatttat
     5161 tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc atattgaaat
     5221 ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat gacaagtcat
     5281 tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat ttaccatatc
     5341 ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct gttgcccctg
     5401 cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg cctttttcaa
     5461 atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg atgacgatac
     5521 caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca gattctgctt
     5581 cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg ttttctgtaa
     5641 agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag ttgttgtctt
     5701 cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt aagtagaagt
     5761 aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata ccgtcgattg
     5821 ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt ttttcagctg
     5881 ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg tggtcaactg
     5941 gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt agccatgaat
     6001 agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt ccgctacgtg
     6061 ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga ttcatgattt
     6121 cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt tcaggtgtca
     6181 cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt ccttctaagt
     6241 ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc attaattttg
     6301 caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat tcataggcaa
     6361 cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc atcaatgttt
     6421 cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa tgggtacgca
     6481 tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt tcaaaactag
     6541 cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag ttttgacgcc
     6601 agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg ccgttctcag
     6661 ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat tcactcatag
     6721 tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac ctgttcttct
     6781 attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta tcaattatcc
     6841 gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc
     6901 ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt tcaaaattgg
     6961 aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct aatcaattac
     7021 tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac catcgaattc
     7081 atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc
     7141 tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg tggaaacaag
     7201 cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt taccgaatcc
     7261 ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg cttgcgttat
     7321 tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt cttctgccaa
     7381 acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta agtcttcgat
     7441 ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc gatcatcttg
     7501 attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa gccaaacggc
     7561 accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg ggatcgttaa
     7621 accgaacgct tctacttctg gtccttctaa ttttcggatt aggtctaatc cagctgtgat
     7681 atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt tatgcagatg
     7741 caaaaagtcg atagattgca gaatttggta agtaaactct gtaaatgaga ttcctacttc
     7801 caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt ttttaccgta
     7861 gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt tcacaaatgt
     7921 gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt ccacgttatg
     7981 ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg ggtcgccgat
     8041 cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt ggaatctttt
     8101 catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc
     8161 gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt cgtttgtttg
     8221 ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac tgacctccgt
     8281 gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt tcttcaggat
     8341 aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat ctcgcggtac
     8401 cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg ctgtcgcgtg
     8461 gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac tggtttgcag
     8521 cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat cttcgtcttg
     8581 ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca gaaaaaggat
     8641 atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg aacattttat
     8701 tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt tatcgagaag
     8761 tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac aagtaaaaaa
     8821 atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt aggagccact
     8881 tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg atcatctaca
     8941 aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat
     9001 gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga ttatgaccaa
     9061 gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca acagcttctt
     9121 caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac tcagatctct
     9181 catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc tcaacaggca
     9241 caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat cgaacaactt
     9301 tatcaaaatg attttgtttt agttaattta aatgatttga tccaaaaaga cgaacaagga
     9361 aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt tctttcacaa
     9421 gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg attggtcgta
     9481 tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga aatcggtgac
     9541 tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt ttcttatcga
     9601 ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta tcgaacatca
     9661 aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg
     9721 gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg tcacttgaat
     9781 atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca gcaagaagtt
     9841 gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga catcagcgac
     9901 tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca aggtttcgat
     9961 attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac ggatttctat
    10021 cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga cggaaaaaat
    10081 cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc acgcgaatct
    10141 gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat cgtcagtttc
    10201 ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat
    10261 tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt
    10321 gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc
    10381 cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc
    10441 gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat
    10501 tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct
    10561 tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat
    10621 gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct
    10681 ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct
    10741 gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt
    10801 tcaaaaccgt tcatttg
//
LOCUS       DANJJW010000091        10817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000091 DANJJW010000000
VERSION     DANJJW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(25..162)
                     /locus_tag="PWS82_002186"
     CDS             complement(25..162)
                     /locus_tag="PWS82_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0907279.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="PWS82_002187"
     CDS             complement(159..1343)
                     /locus_tag="PWS82_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0907280.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="PWS82_002188"
     CDS             complement(1599..1853)
                     /locus_tag="PWS82_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907281.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="PWS82_002189"
     CDS             complement(1878..3020)
                     /locus_tag="PWS82_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL0907282.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="PWS82_002190"
     CDS             complement(3080..4432)
                     /locus_tag="PWS82_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0907283.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="PWS82_002191"
     CDS             complement(4503..4847)
                     /locus_tag="PWS82_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0907284.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="PWS82_002192"
     CDS             complement(4857..5231)
                     /locus_tag="PWS82_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0907285.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="PWS82_002193"
     CDS             complement(5244..5558)
                     /locus_tag="PWS82_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0907286.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWS82_002194"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWS82_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="PWS82_002195"
     CDS             complement(8976..9638)
                     /locus_tag="PWS82_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907287.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="PWS82_002196"
     CDS             10255..10485
                     /locus_tag="PWS82_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907288.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="PWS82_002197"
     CDS             10491..10790
                     /locus_tag="PWS82_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907289.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DANJJW010000092        10531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000092 DANJJW010000000
VERSION     DANJJW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            256..531
                     /locus_tag="PWS82_002198"
     CDS             256..531
                     /locus_tag="PWS82_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907290.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="PWS82_002199"
     CDS             544..2616
                     /locus_tag="PWS82_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL0907291.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="PWS82_002200"
     CDS             2781..4667
                     /locus_tag="PWS82_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0907292.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="PWS82_002201"
     CDS             4679..5626
                     /locus_tag="PWS82_002201"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL0907293.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="PWS82_002202"
     CDS             5646..6173
                     /locus_tag="PWS82_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0907294.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="PWS82_002203"
     CDS             complement(6236..6889)
                     /locus_tag="PWS82_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL0907295.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="PWS82_002204"
     CDS             7021..7863
                     /locus_tag="PWS82_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0907296.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="PWS82_002205"
     CDS             8021..8917
                     /locus_tag="PWS82_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0907297.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="PWS82_002206"
     CDS             8920..9633
                     /locus_tag="PWS82_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL0907298.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="PWS82_002207"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="PWS82_002207"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0907299.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="PWS82_002208"
     CDS             10168..10395
                     /locus_tag="PWS82_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL0907300.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         3656 a         1786 c         2173 g         2916 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg g
//
LOCUS       DANJJW010000093        10209 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000093 DANJJW010000000
VERSION     DANJJW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            142..291
                     /locus_tag="PWS82_002209"
     CDS             142..291
                     /locus_tag="PWS82_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL0907301.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            302..1816
                     /gene="dltA"
                     /locus_tag="PWS82_002210"
     CDS             302..1816
                     /gene="dltA"
                     /locus_tag="PWS82_002210"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL0907302.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1813..3018
                     /gene="dltB"
                     /locus_tag="PWS82_002211"
     CDS             1813..3018
                     /gene="dltB"
                     /locus_tag="PWS82_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL0907303.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3063..3296
                     /gene="dltC"
                     /locus_tag="PWS82_002212"
     CDS             3063..3296
                     /gene="dltC"
                     /locus_tag="PWS82_002212"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL0907304.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3299..4567
                     /gene="dltD"
                     /locus_tag="PWS82_002213"
     CDS             3299..4567
                     /gene="dltD"
                     /locus_tag="PWS82_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL0907305.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4708..5790
                     /locus_tag="PWS82_002214"
     CDS             4708..5790
                     /locus_tag="PWS82_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0907306.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5790..6593
                     /locus_tag="PWS82_002215"
     CDS             5790..6593
                     /locus_tag="PWS82_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0907307.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6663..7403
                     /locus_tag="PWS82_002216"
     CDS             6663..7403
                     /locus_tag="PWS82_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0907308.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7414..8418
                     /locus_tag="PWS82_002217"
     CDS             7414..8418
                     /locus_tag="PWS82_002217"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL0907309.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8463..10160)
                     /locus_tag="PWS82_002218"
     CDS             complement(8463..10160)
                     /locus_tag="PWS82_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907310.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3269 a         1693 c         2076 g         3171 t
ORIGIN      
        1 agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt agtaaaaaaa
       61 cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac tttgtgtgag
      121 gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt gaattattgg
      181 ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct gatttatctg
      241 tatcactata aaaatattca aggaggaact tttatctata acgaatttta ggaggaagag
      301 tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg aacctgatcg
      361 agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag catcagacgc
      421 acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa
      481 tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc atgcctattt
      541 gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg tggctaaacc
      601 atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc ctgtcataac
      661 aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca gaaatagcgt
      721 aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag aaccaaaagg
      781 cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg aagactttgg
      841 gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt tatcggtatt
      901 ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata aagcagttgt
      961 tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg tgtgggtttc
     1021 tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc
     1081 ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg cagaaagtct
     1141 acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag aagcaactgt
     1201 agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc gactaccgat
     1261 tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa
     1321 aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt acctgaacaa
     1381 tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt accatacagg
     1441 ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta tggatcagca
     1501 ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc tgctccaaga
     1561 caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct
     1621 ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt tgactaaatc
     1681 tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga agttccatta
     1741 tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat taacagctga
     1801 ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt ttattttttt
     1861 cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga aaaaagatta
     1921 ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg aggtaattat
     1981 tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact tgtatggggc
     2041 tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc cgttatttta
     2101 gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg aaaagcatcg
     2161 ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat gatcatggaa
     2221 ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt tctgctgttc
     2281 tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa agatcagctc
     2341 catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca aaatattttt
     2401 gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact gcttcctgta
     2461 gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg ttatatgtac
     2521 gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc agtcggtaca
     2581 agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt atcatggaat
     2641 atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg tgattatgta
     2701 tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg
     2761 tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg attgacgtgg
     2821 tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga tgcatggatc
     2881 cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca tggcttagcc
     2941 gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg atttttagat
     3001 aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag gagaaaagga
     3061 aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat gaagtaagaa
     3121 aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca tctgttcaat
     3181 tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa ttcgagcgag
     3241 aggattggag cacaccgaat aaaatcattc aacaagcaac tgcattgaag ggttgagtat
     3301 gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct tactgtttgt
     3361 gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta gacgtgcctc
     3421 ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg cattatctga
     3481 aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc catttcatcc
     3541 gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg gagcacccgg
     3601 gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta tgaaaaacaa
     3661 gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta
     3721 ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa tgaagaaagt
     3781 aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag taaaagagaa
     3841 tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt tacctgaatc
     3901 tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag tattcagcaa
     3961 tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca
     4021 ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag aaacgacgaa
     4081 taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc acgagccaga
     4141 gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt ctgattttca
     4201 gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca tcccaccggt
     4261 taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag gatttgccaa
     4321 aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt ttgtcaatca
     4381 agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag gctggttggc
     4441 tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta gtaagtatca
     4501 tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata aactacaatt
     4561 aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga atattctgaa
     4621 taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt ataatcacaa
     4681 tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct acttattgat
     4741 ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt attcagagaa
     4801 tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt gattgccaaa
     4861 catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga agttggtttt
     4921 atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg aggttggtgg
     4981 gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac
     5041 ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa taaaccttat
     5101 gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc caaagagtgg
     5161 ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat gaatggcagt
     5221 aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc tctgcgagta
     5281 ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag cgatgtgcaa
     5341 gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc cgatattgct
     5401 tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa agaagcagat
     5461 tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat cccgcataaa
     5521 aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt ccaatataca
     5581 gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga tggtgttccg
     5641 tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt gattcatgaa
     5701 gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct tgatagtgaa
     5761 acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt tctgaatatc
     5821 ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg cttgttttct
     5881 tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt ccttttccgg
     5941 aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt ttattcgcat
     6001 tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga ccaaaaagta
     6061 accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc atcaatttga
     6121 tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg tttttaatca
     6181 gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct acgggtgtca
     6241 tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg atttttgttt
     6301 cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg acaaagttcg
     6361 actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta ttgaaagata
     6421 gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa
     6481 tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat
     6541 tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact tagcatgtaa
     6601 gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag ggggaatgta
     6661 cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat caagggacaa
     6721 caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt agtgctacgc
     6781 tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc cttagccatt
     6841 atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa cttcatccta
     6901 agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc attcactttg
     6961 aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct gaagaattaa
     7021 aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta tgttacgcca
     7081 tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat tcaggttatt
     7141 tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat acctatcttt
     7201 ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga gaacttgcga
     7261 aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt gatttagaac
     7321 aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa cttgctgcag
     7381 ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa tgaaaaccac
     7441 gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc tttagatgaa
     7501 cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa ccaaaatacg
     7561 cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta
     7621 agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt tatcatgcct
     7681 gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat ccaagcactt
     7741 catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt cattgatggc
     7801 atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc tcatggaaca
     7861 attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa
     7921 atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa accatttctt
     7981 ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt
     8041 ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga agattggaaa
     8101 aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta cgggaagtct
     8161 ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga agtccatttg
     8221 aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt cggactagga
     8281 gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc atctctatca
     8341 aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc agaagaattg
     8401 cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga ttccgctatt
     8461 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
     8521 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
     8581 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
     8641 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
     8701 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
     8761 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
     8821 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
     8881 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
     8941 aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa atcttgcaga
     9001 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
     9061 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
     9121 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
     9181 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
     9241 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
     9301 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
     9361 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
     9421 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
     9481 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
     9541 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
     9601 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
     9661 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
     9721 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
     9781 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
     9841 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
     9901 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
     9961 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
    10021 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
    10081 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
    10141 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
    10201 taagcaatt
//
LOCUS       DANJJW010000094        10195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000094 DANJJW010000000
VERSION     DANJJW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            120..626
                     /locus_tag="PWS82_002219"
     CDS             120..626
                     /locus_tag="PWS82_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL0907311.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="PWS82_002220"
     CDS             643..1542
                     /locus_tag="PWS82_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL0907312.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="PWS82_002221"
     CDS             1629..2513
                     /locus_tag="PWS82_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0907313.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="PWS82_002222"
     tRNA            complement(2705..2776)
                     /locus_tag="PWS82_002222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="PWS82_002223"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="PWS82_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL0907314.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="PWS82_002224"
     CDS             4319..5410
                     /locus_tag="PWS82_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL0907315.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="PWS82_002225"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="PWS82_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0907316.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="PWS82_002226"
     CDS             6562..7752
                     /locus_tag="PWS82_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL0907317.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="PWS82_002227"
     CDS             7856..8611
                     /locus_tag="PWS82_002227"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL0907318.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="PWS82_002228"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="PWS82_002228"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL0907319.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3566 a         1886 c         2112 g         2631 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cagat
//
LOCUS       DANJJW010000095        10086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000095 DANJJW010000000
VERSION     DANJJW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(247..759)
                     /locus_tag="PWS82_002229"
     CDS             complement(247..759)
                     /locus_tag="PWS82_002229"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL0907320.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWS82_002230"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWS82_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL0907321.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="PWS82_002231"
     CDS             complement(3212..3655)
                     /locus_tag="PWS82_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL0907322.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="PWS82_002232"
     CDS             complement(3656..4444)
                     /locus_tag="PWS82_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0907323.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="PWS82_002233"
     CDS             complement(4441..5382)
                     /locus_tag="PWS82_002233"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL0907324.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="PWS82_002234"
     CDS             complement(5559..6146)
                     /locus_tag="PWS82_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL0907325.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWS82_002235"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWS82_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL0907326.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="PWS82_002236"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="PWS82_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0907327.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="PWS82_002237"
     CDS             complement(9063..9998)
                     /locus_tag="PWS82_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0907328.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DANJJW010000096        10084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000096 DANJJW010000000
VERSION     DANJJW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            162..392
                     /locus_tag="PWS82_002238"
     CDS             162..392
                     /locus_tag="PWS82_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL0907329.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="PWS82_002239"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="PWS82_002239"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0907330.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="PWS82_002240"
     CDS             1803..1979
                     /locus_tag="PWS82_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0907331.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="PWS82_002241"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="PWS82_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL0907332.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="PWS82_002242"
     CDS             2474..2941
                     /locus_tag="PWS82_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907333.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="PWS82_002243"
     CDS             3026..3910
                     /locus_tag="PWS82_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0907334.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="PWS82_002244"
     CDS             4024..4260
                     /locus_tag="PWS82_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL0907335.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="PWS82_002245"
     tRNA            4348..4428
                     /locus_tag="PWS82_002245"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="PWS82_002246"
     tRNA            4435..4506
                     /locus_tag="PWS82_002246"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="PWS82_002247"
     CDS             4631..4801
                     /locus_tag="PWS82_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907336.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="PWS82_002248"
     CDS             5021..5728
                     /locus_tag="PWS82_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907337.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="PWS82_002249"
     CDS             complement(5788..6432)
                     /locus_tag="PWS82_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL0907338.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="PWS82_002250"
     CDS             6559..6846
                     /locus_tag="PWS82_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907339.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="PWS82_002251"
     CDS             6954..7274
                     /locus_tag="PWS82_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907340.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="PWS82_002252"
     CDS             7355..8668
                     /locus_tag="PWS82_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0907341.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="PWS82_002253"
     CDS             8665..9384
                     /locus_tag="PWS82_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL0907342.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="PWS82_002254"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="PWS82_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL0907343.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DANJJW010000097         9909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000097 DANJJW010000000
VERSION     DANJJW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(373..597)
                     /locus_tag="PWS82_002255"
     CDS             complement(373..597)
                     /locus_tag="PWS82_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907344.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            788..3565
                     /locus_tag="PWS82_002256"
     CDS             788..3565
                     /locus_tag="PWS82_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0907345.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3646..4065
                     /locus_tag="PWS82_002257"
     CDS             3646..4065
                     /locus_tag="PWS82_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL0907346.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4077..4547
                     /locus_tag="PWS82_002258"
     CDS             4077..4547
                     /locus_tag="PWS82_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907347.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4564..5343
                     /locus_tag="PWS82_002259"
     CDS             4564..5343
                     /locus_tag="PWS82_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907348.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5330..6151
                     /locus_tag="PWS82_002260"
     CDS             5330..6151
                     /locus_tag="PWS82_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0907349.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6168..7217
                     /locus_tag="PWS82_002261"
     CDS             6168..7217
                     /locus_tag="PWS82_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0907350.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7230..8234
                     /locus_tag="PWS82_002262"
     CDS             7230..8234
                     /locus_tag="PWS82_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0907351.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8867..9886
                     /locus_tag="PWS82_002263"
     CDS             8867..9886
                     /locus_tag="PWS82_002263"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0907352.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3322 a         1643 c         2073 g         2871 t
ORIGIN      
        1 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
       61 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
      121 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
      181 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
      241 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
      301 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
      361 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
      421 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
      481 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
      541 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
      601 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
      661 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
      721 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
      781 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
      841 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
      901 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
      961 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
     1021 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
     1081 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
     1141 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
     1201 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
     1261 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
     1321 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
     1381 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
     1441 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
     1501 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
     1561 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
     1621 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
     1681 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
     1741 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
     1801 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
     1861 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
     1921 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
     1981 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
     2041 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
     2101 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
     2161 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
     2221 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
     2281 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
     2341 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
     2401 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
     2461 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
     2521 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
     2581 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
     2641 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
     2701 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
     2761 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
     2821 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
     2881 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
     2941 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
     3001 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
     3061 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
     3121 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
     3181 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
     3241 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
     3301 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
     3361 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
     3421 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
     3481 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
     3541 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
     3601 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
     3661 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
     3721 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
     3781 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
     3841 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
     3901 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
     3961 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
     4021 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
     4081 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
     4141 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
     4201 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
     4261 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
     4321 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
     4381 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
     4441 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
     4501 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
     4561 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
     4621 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
     4681 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
     4741 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
     4801 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
     4861 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
     4921 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
     4981 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
     5041 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
     5101 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
     5161 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
     5221 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
     5281 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
     5341 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
     5401 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
     5461 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
     5521 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
     5581 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
     5641 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
     5701 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
     5761 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
     5821 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
     5881 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
     5941 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
     6001 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
     6061 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
     6121 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
     6181 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
     6241 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
     6301 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
     6361 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
     6421 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
     6481 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
     6541 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
     6601 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
     6661 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
     6721 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
     6781 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
     6841 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
     6901 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
     6961 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
     7021 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
     7081 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
     7141 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
     7201 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
     7261 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
     7321 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
     7381 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
     7441 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
     7501 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
     7561 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
     7621 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
     7681 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
     7741 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
     7801 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
     7861 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
     7921 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
     7981 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
     8041 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
     8101 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
     8161 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
     8221 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
     8281 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
     8341 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
     8401 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
     8461 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
     8521 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
     8581 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
     8641 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
     8701 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
     8761 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
     8821 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
     8881 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
     8941 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
     9001 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
     9061 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
     9121 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
     9181 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
     9241 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
     9301 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
     9361 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
     9421 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
     9481 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
     9541 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
     9601 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
     9661 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
     9721 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
     9781 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
     9841 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
     9901 gttaatcgt
//
LOCUS       DANJJW010000098         9837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000098 DANJJW010000000
VERSION     DANJJW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            116..595
                     /locus_tag="PWS82_002264"
     CDS             116..595
                     /locus_tag="PWS82_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907353.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            628..2856
                     /locus_tag="PWS82_002265"
     CDS             628..2856
                     /locus_tag="PWS82_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0907354.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2985..4628
                     /locus_tag="PWS82_002266"
     CDS             2985..4628
                     /locus_tag="PWS82_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0907355.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4701..5246)
                     /locus_tag="PWS82_002267"
     CDS             complement(4701..5246)
                     /locus_tag="PWS82_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL0907356.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5269..5754)
                     /locus_tag="PWS82_002268"
     CDS             complement(5269..5754)
                     /locus_tag="PWS82_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0907357.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5744..6556)
                     /locus_tag="PWS82_002269"
     CDS             complement(5744..6556)
                     /locus_tag="PWS82_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL0907358.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6695..8227)
                     /locus_tag="PWS82_002270"
     CDS             complement(6695..8227)
                     /locus_tag="PWS82_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL0907359.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8336..9367)
                     /locus_tag="PWS82_002271"
     CDS             complement(8336..9367)
                     /locus_tag="PWS82_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0907360.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3271 a         1823 c         1893 g         2850 t
ORIGIN      
        1 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
       61 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
      121 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
      181 tggaagaaat gcattaaata tggatgcaaa agggctttca ctgggccttg ctcagtggaa
      241 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
      301 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcgca
      361 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
      421 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
      481 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
      541 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
      601 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
      661 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
      721 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
      781 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
      841 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
      901 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
      961 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
     1021 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
     1081 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
     1141 gaaaaaggct atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
     1201 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
     1261 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
     1321 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
     1381 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
     1441 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
     1501 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
     1561 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
     1621 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
     1681 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aagaaagaac cattagcgcc
     1741 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
     1801 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
     1861 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
     1921 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
     1981 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
     2041 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
     2101 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
     2161 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
     2221 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
     2281 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
     2341 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
     2401 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
     2461 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
     2521 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
     2581 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
     2641 aaagcattgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
     2701 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
     2761 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
     2821 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
     2881 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
     2941 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
     3001 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
     3061 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
     3121 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
     3181 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
     3241 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
     3301 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
     3361 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
     3421 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
     3481 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
     3541 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
     3601 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
     3661 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
     3721 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
     3781 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
     3841 aagggcaatt agatgtcctt aaattgaaac atgcattaag cgcttggcaa acttcattgg
     3901 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
     3961 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
     4021 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
     4081 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
     4141 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
     4201 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
     4261 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
     4321 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
     4381 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
     4441 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
     4501 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
     4561 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
     4621 acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat
     4681 tgctctgtca attactcatt tcaataattc gctctaggac caccgatata tttaggacga
     4741 gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact
     4801 tggacatgct tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca
     4861 actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca
     4921 cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact
     4981 gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt
     5041 tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg
     5101 gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga
     5161 tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct
     5221 tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt
     5281 ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag
     5341 attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca
     5401 accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca
     5461 catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac
     5521 taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga
     5581 agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc
     5641 ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag
     5701 ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca
     5761 ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc
     5821 cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg
     5881 cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg
     5941 ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat
     6001 ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg
     6061 aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac
     6121 cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt
     6181 cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca
     6241 aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca
     6301 tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat
     6361 caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct
     6421 cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct
     6481 caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg
     6541 tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa
     6601 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
     6661 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
     6721 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
     6781 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
     6841 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
     6901 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
     6961 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
     7021 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
     7081 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
     7141 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
     7201 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
     7261 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
     7321 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
     7381 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
     7441 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
     7501 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
     7561 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
     7621 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
     7681 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
     7741 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
     7801 taggaatctg ttttgatttt gttccatttg aaatttctct cactcgcttt tttgtctcat
     7861 catcaaataa aatgaaaggg tcttctaaac gatcgataaa tacttccatt gttgttgttg
     7921 gtagatgata agaagtcatg acaggcactg caccaaggta agacacagaa acagctagaa
     7981 ggtatgtgtc aaaagcggaa ctcttataaa taataacttt gtctccggat ttcactccaa
     8041 gatgagccaa ttgataagcc cgtttcaaca cactttcgca gcattttttg taagttgttt
     8101 caagtcctaa ttctgggaaa gctgaaagtg gctcatcaaa gataatcggg gtctcggaaa
     8161 agttagctgc agcttcttga aaattcgtaa ataagttcaa aggggaatac gttatggtct
     8221 ccatcattca cactccatta tcttttgtta agtaatatac aaattcaaga taatcgtaac
     8281 ataatccttt atataataac atagtttatt gtgaaaaaaa gatcatacat ttgttttata
     8341 tggaagaaac ccgaacgaat gccgctaatt cgtgataaga aaaatcaacc atcttttccc
     8401 agatatcttg aaaagaatgg gatgagaggt atcgattacc acataaataa tttaccgcgt
     8461 cagcatcatc taaatatagt tctgcataat aacgatatgt ttttctgcat agttctggcg
     8521 atacgttttt atatactgta attttgctca aaatttggcg aatagtccga ggagaaattt
     8581 gctgatcatg tgaggaacga aaaacaaaag aattttcgtc cgtattttgt gctgttaact
     8641 taaaatagtt ctccagaaat ggcatcgctt cttttggtat tggtacttta ttcagtctgt
     8701 tttcttttct attcagatta agaatctgct tggtcaaaga tacatcttta cgtttaagtt
     8761 ctactacttc atggatcttt aatccgcaat acagaagaat ggcagtaatt gcaaggttcc
     8821 tcggtctatt cttttcttca aagctagctt ctctgcttgt ttgaaagcct tcaatcggga
     8881 taaacaaatg ctcaaaatct ttgaaaagtt ctgcatcgat atatcgcgga gctgctttgg
     8941 ttgtttccgg taaacttgta cccaaaagag aaatctcttc ttcaatcagt cctttttgat
     9001 acaaaaatcg taaaaaatga tttaatgaat attgcttcct tgaaatcccg taatagctat
     9061 tttcaaacaa ttgctccgtt tcttggttcg ctgtagtttg gaacgaacga gtatgtcgat
     9121 agattttagc taagaaattc tgataatctt gtttttctaa atggataatc agctgatcat
     9181 ccacttcttc agaaaatact tttctcaaat aatcagtaaa cagatgaaaa tcttttgcgt
     9241 attcatagcg actgcctaat gaaagggaag aacgattttc ttctaaaaac tgttgaaaga
     9301 cggaagggag caagtgtgta cgttgcttta acttttcttg cagttgtttt ctagttttgc
     9361 tgtccattac aaattccctc caatagtgtt cattttctat attttaccgc atttcatcaa
     9421 atgaagatag acagtttgtc ttgatcttag gaagcaaatt tttaactaag tactagctaa
     9481 aagatatgaa gtaagaagat acatttttag ttattttatt agtgcagtca ttcttttgat
     9541 cttttaattg atttatcctc tttttagcta tgtggttact tcatgagcaa tgaaatggat
     9601 ttggttcgtt ctaatggcag gaggctcttt cttttatttc cgaaaaatta gaaaacattt
     9661 tttcagaatc gattggctct ccatttattc aataatggtt tgtctaacga tattgttggg
     9721 ataattctta aagaagtatt agaaatagtt ggtacttcat tactttacat gaagatttat
     9781 tctatggtag gagcaattat attgagaatt aatccttttt tatataagtt gtttgct
//
LOCUS       DANJJW010000099         9820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000099 DANJJW010000000
VERSION     DANJJW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..195)
                     /locus_tag="PWS82_002272"
     CDS             complement(<1..195)
                     /locus_tag="PWS82_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907361.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="PWS82_002273"
     CDS             complement(211..825)
                     /locus_tag="PWS82_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL0907362.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="PWS82_002274"
     CDS             complement(972..1655)
                     /locus_tag="PWS82_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0907363.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="PWS82_002275"
     CDS             complement(1764..2222)
                     /locus_tag="PWS82_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL0907364.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="PWS82_002276"
     CDS             complement(2306..4069)
                     /locus_tag="PWS82_002276"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL0907365.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="PWS82_002277"
     CDS             complement(4290..5750)
                     /locus_tag="PWS82_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907366.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PWS82_002278"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PWS82_002278"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL0907367.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="PWS82_002279"
     CDS             complement(6907..8334)
                     /locus_tag="PWS82_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0907368.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="PWS82_002280"
     CDS             complement(8365..9579)
                     /locus_tag="PWS82_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL0907369.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DANJJW010000100         9703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000100 DANJJW010000000
VERSION     DANJJW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            13..2340
                     /locus_tag="PWS82_002281"
     CDS             13..2340
                     /locus_tag="PWS82_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0907370.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2466..2795
                     /locus_tag="PWS82_002282"
     CDS             2466..2795
                     /locus_tag="PWS82_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0907371.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2835..3839
                     /locus_tag="PWS82_002283"
     CDS             2835..3839
                     /locus_tag="PWS82_002283"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0907372.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3836..4282
                     /locus_tag="PWS82_002284"
     CDS             3836..4282
                     /locus_tag="PWS82_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0907373.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4432..5247
                     /locus_tag="PWS82_002285"
     CDS             4432..5247
                     /locus_tag="PWS82_002285"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0907374.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5453..6625
                     /locus_tag="PWS82_002286"
     CDS             5453..6625
                     /locus_tag="PWS82_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0907375.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6787..7281)
                     /locus_tag="PWS82_002287"
     CDS             complement(6787..7281)
                     /locus_tag="PWS82_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907376.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            7864..8286
                     /locus_tag="PWS82_002288"
     CDS             7864..8286
                     /locus_tag="PWS82_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907377.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8452..9645)
                     /locus_tag="PWS82_002289"
     CDS             complement(8452..9645)
                     /locus_tag="PWS82_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0907378.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT         3317 a         1865 c         1867 g         2654 t
ORIGIN      
        1 ttgagaggag ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa
       61 aaacattttg tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg
      121 atttcgatca tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt
      181 tatacagaaa cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat
      241 tctgaaatac agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc
      301 acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca
      361 ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact
      421 gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc caaagatgga
      481 gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc
      541 aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat tggctccaca
      601 gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga
      661 aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca
      721 tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca
      781 aatgatattc cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga
      841 aatgtttcaa attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca
      901 ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca
      961 ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat
     1021 tcttttgcac aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc
     1081 tatacaggca aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt
     1141 gctccttcta aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat
     1201 acgcctacta tcaacggata tacctttaca gctgtttcag cgatagaaac aaagaaccaa
     1261 tcagcaatga gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca
     1321 aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc
     1381 aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc
     1441 tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc
     1501 acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct
     1561 actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct
     1621 gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa
     1681 atcagcggtg cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg
     1741 tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca
     1801 cttagcagta tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc
     1861 agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag
     1921 ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca
     1981 gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt
     2041 ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt
     2101 aacagccgga cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta
     2161 aacaataatt atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc
     2221 ttgcttcctt cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt
     2281 attttttctg gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga
     2341 aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct
     2401 tttttatagg ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata
     2461 atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc
     2521 cggataactt tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct
     2581 cggatgaatt ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac
     2641 tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg
     2701 aggaagaggg cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg
     2761 tttaccgatt tacggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg
     2821 tcgttgtgct tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc
     2881 caaagagcga acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc
     2941 agacagattt tgcagatggg gataaagtcc cttatctcat caccgtaaca attcctgaga
     3001 acatcaacga gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag
     3061 aaccagaaac gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa
     3121 aaataaaaca tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggaa
     3181 aaaaattaac gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg
     3241 ataacgatgt acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa
     3301 caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc
     3361 aatttctagt caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg
     3421 tagccaaaga tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg
     3481 tccatggatt gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata
     3541 tattgagtca atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc
     3601 ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc
     3661 cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa
     3721 caagcgaaga gatgcttgga ggtttctcaa ttttagggtt attgctagtc ctttcaacag
     3781 ggacagcttg gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa
     3841 aaacatcaaa acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt
     3901 tctcggccta ggaactgcag aggtttctgc agccgtatca cctacaccag aaaatgttac
     3961 ggttaatttg cataaattga aattcacttc agcaccagaa aatcaaataa acaacggaac
     4021 ggaattgaca tttccgaata gcgaaccgtt gaacggcgtt gaattcaacg tatatgacat
     4081 cactgctacg tactatccaa gtaaagacac agcagttcca gcagacgcta cacccttcgc
     4141 aagtgtaacg acttctggtg aaggattagc taacctgact cttccaggta aatctgatgg
     4201 gaaagatgct gtatacgtct ttgtagaaac accaaagccc ggtgtcgaaa cgtcgccaaa
     4261 tatcgtgtta agcttaccct agaccctgac aaacctttag acacgattca cctatatgct
     4321 aaaaatgtca tcaaagaaag caatatcaaa atccagaaag ctgggaactt ttcaggggta
     4381 gataagttag caggggcgca attccagatc aaaaattcag agaatcaatt cgtgaccggt
     4441 tttgatgaaa acactggtgt cgctctctat gatcctgctg agtcagcgtt gacattcaca
     4501 actgatactg aaggatccat cagtgtcaat ggattaatag acggcaatta tactttagtt
     4561 gaaacaaaag caccagatgg ttatcaatta accaatccac aaaaagaaac tacttttata
     4621 gtaaaaaacg gaaaattgca acaaacaacc ggtatcatcc atggcgcaga tgaagccaat
     4681 cttgggaatc caaacggtgt agtcaatcgg gaaaatacaa ctacggataa cacgattact
     4741 gtagaaaatg tccaaaacta cggtgatttc cacttcatca aacaagatgt gaacacgcaa
     4801 gaaaaattag ccgatgcaga attttctatt ctaagagcaa aagatccaac tgttaaatta
     4861 atgaaaaaaa tagcaaacct tgcctcagga gaatacccat atgcttggtc agatgaagtc
     4921 acaggttctg actgggaaga agtgaaattg atttctgatt cagaagggaa attttcaatc
     4981 gaagggctga aacaaggggc atactgcctt caagaaacaa aagcaccaga aggctacgca
     5041 ttacctaaga atgaagctgc gtacactgaa tttacagtag atgatgatcc aacaacttct
     5101 gatgaaacaa cgatcgataa ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg
     5161 atcatcgtat tcatcttggt aggtactgct ttagttggag gagcagtgat ctatttcaaa
     5221 aaacgccatc aagaaacaga agcttaaact attggaaaca aaaatcatac gataaataaa
     5281 cgatagtgat tcaaatgggg agaaaggttg tgacaagtgg tcggtctctt ggtattcgct
     5341 caacaaacgg caaaatagga ggaactattt tgccgtttgc ttcgctaata ctcaggtcaa
     5401 ggcgctgatt tcacaacctc ttcatataca tattagaaaa taggtggaca tgatgaataa
     5461 aagcagaata agaatactgg acatcttcat ggcaatcatt ttagtcgttg ggatcgggat
     5521 cttttcttat ccttttgtag aagattcact gaatgatttt cttgcccagc aaatgatcat
     5581 ccattatcaa aagcaagcat cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa
     5641 aatgacaaag aaaaaccaac agttggcaga gcaaaacgta tcaccaggga taggcagttt
     5701 caaccaagca gttgatgcaa aagcattaaa agatttacct agtaatgcct tttttatgga
     5761 acatatgctt ggagtcatcg aaatcccaaa aatcaatgtc agcttaccta tattcgatca
     5821 gacaacggaa atatttttgc aaaaaggaac gtcattattg gaaggaagtt cttatccgac
     5881 tggcgggaaa aacacccatg ccgttctttc tgggcatcgt ggactcccag aagcaaaatt
     5941 atttacagat ttgcctaagt tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa
     6001 gacattggcg tatcaagtag aaaaaatcca agtggtctta cctgacgaag tagattcttt
     6061 gggtatccaa aaaggcagag atctcgtgac tttgcttacc tgcactcctt atatggtcaa
     6121 tacacatcga ctgcttgtga cggggcatcg tattccttat caagcaaaag aggcaaaaaa
     6181 agcgatccaa ggaattgatc aatggaaaaa atggaaattt tttatttggt ttatcggtat
     6241 actacttgga tcaatcggct tagtttggct actcatcgct tatttagatt ctttggccat
     6301 cgctaagcga aactatcctt tatcttttta tgtaaagaac acaaacggac gaccaatcga
     6361 aggaatggtc ttttctgtaa aaacgctcaa cggcaaacac tatattacta gagaaaaagt
     6421 tccatttgtg aaagcttctg atgaatacgg actagtgaga ttttctgatt taaaaggcag
     6481 gaactatcgc ttgcaacatg aggagctcct gcttaaaatc catgtaaaac ataaacattc
     6541 gaaacagttt tcaatgaaat tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt
     6601 gtactatttg attgagaaag aatgaatgaa gatgtccaaa aaagcagagg gaacagacta
     6661 aatgaaaaaa gagagaagct gacgaaatgg acgtgtaaac tgctattttt ggtttactat
     6721 ctggaacgcc agcttctatt tttttatgtt tcagaagaat tttattatta tagtataact
     6781 tttgaattag tttccataac atttggagca aggggttaaa ccacgagctt gagcttcgct
     6841 taaagtggca ggggtatacg taccatttcc acatttatgt gtatggtatt ttgaaccggt
     6901 cggtgtgaca tagacagttt gttcattatt ttgctcagga gctgcggctt gtgcctgctg
     6961 agaagcctgt tcagctgctg cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc
     7021 ttcttgcctc gcctgttctg cttgttgctg tgcgattgct tcttgttgcg cttgttctgc
     7081 ttgttgtcgt gcaatttctt cttgcttttt ccgttcttct tctgcttctt ttgctttgat
     7141 tgtggaatca attgcagcaa gacggcttgt caactcttgt ttgttttcag gtagcgcttg
     7201 gatagctgct gaagcagttg catagttttc ttttgtagga tttgcttcag cttgtgacaa
     7261 agcagattcc gcttctgtca cttttttttc tagtgcagct ttttccgctt tttgtttctc
     7321 ttcttctttt ttcttttcaa tttgttcttt cgatgaatct gccgtacttt cggagatcgt
     7381 ggtactgctg ctggaagcct gagctgaact tgtgctttga gtagtagacg tagtagcacc
     7441 acacccactc aacaaacttc ctgcgaccaa aaacaacgca actaattttt tcactaaatt
     7501 tcctccttaa gtaaaaaaca accgttttca ataaatgtcc ttttcaattg tttacttact
     7561 tgttataata attgtgttat gaatagtgat tgaggaaact taatctaatc ccgtgttgat
     7621 acacgggatt ttttgctata gttctgttat taagtatgca ataataaaat atgtacgtta
     7681 ttttattatt agattattga catttatata ataaacgatt aaatgattca tatcaataga
     7741 atacttagga aaaaaagaga aataataaca ggtttatgca ctattgacgg tgtgacaata
     7801 tgatatttgt atccgttttt ttcttgtatg tagtatagta aagtagatac aggaggagaa
     7861 aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat tcatttactg
     7921 cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa ggtatcaagt
     7981 aagtctatct tgaattatct aacagattta gaagaattgt tccaacaata tcctgataag
     8041 gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga gaacttccca
     8101 atctacatta tttatcttca tttctacaga aaatcatata attatcattt gattgaattc
     8161 atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata tacgagtgta
     8221 tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac ggacgtcaaa
     8281 gaatagtcgc cgccagatag aaggaaatta gcagttgaaa aagctgccct gcatcaagta
     8341 atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt cttattaccg
     8401 cagatgcaag ctttttccac acctctttct tgtcatttac agactcaact atcaatgctg
     8461 caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca gactgactag
     8521 gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca tcaagaaacc
     8581 gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataacga aattattcgc
     8641 cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc caccatacat
     8701 gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact ggctatgggc
     8761 taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa aggctagttt
     8821 aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca tgcctaaaat
     8881 agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat tttgcataat
     8941 tgaaggtagc caattcatta acccaaaata gccggcaatt tggacagtta ccatgatact
     9001 aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa gtttggtttt
     9061 cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc gacgaatgaa
     9121 caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac caaacgttgg
     9181 aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac caataccagc
     9241 aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca aggacataca
     9301 cgccccatat tctcctcccg ctcctacacc tgcgataaag cgaaagagat aaaccaagta
     9361 gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga ccgtttgaga
     9421 aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac ctccaatgag
     9481 catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa tatgaaaaga
     9541 agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct ctaaagcaat
     9601 tcccgccgat gtcgatacta atacctttcg ctgatacgtt gtcattttta tacccctttt
     9661 cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaa
//
LOCUS       DANJJW010000101         9612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000101 DANJJW010000000
VERSION     DANJJW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(226..648)
                     /locus_tag="PWS82_002290"
     CDS             complement(226..648)
                     /locus_tag="PWS82_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0907379.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(638..2812)
                     /locus_tag="PWS82_002291"
     CDS             complement(638..2812)
                     /locus_tag="PWS82_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL0907380.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2828..5170)
                     /locus_tag="PWS82_002292"
     CDS             complement(2828..5170)
                     /locus_tag="PWS82_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907381.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5192..7735)
                     /locus_tag="PWS82_002293"
     CDS             complement(5192..7735)
                     /locus_tag="PWS82_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0907382.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7745..8143)
                     /locus_tag="PWS82_002294"
     CDS             complement(7745..8143)
                     /locus_tag="PWS82_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907383.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8224..8508)
                     /locus_tag="PWS82_002295"
     CDS             complement(8224..8508)
                     /locus_tag="PWS82_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907384.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8532..9530)
                     /locus_tag="PWS82_002296"
     CDS             complement(8532..9530)
                     /locus_tag="PWS82_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907385.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2753 a         2035 c         1492 g         3332 t
ORIGIN      
        1 gatttcaaac aaaatacgtt tattttttct attgatttta gctcaaaatc gattcaattc
       61 gagtgaaatt tgcttcaatt ttcaattggt ttttgttcaa attttgaaac atttttgctc
      121 aaaaaatgag tgaatttgtt tcaaaagaaa aacaaatacg atcgaaaaaa atttgaactc
      181 ataacaattt tttgtttttt tcaacgtatt ctctttcaat tttcactaaa aattaggaag
      241 aaattgattc aatgtctttt caaaaaagaa tgtttttctc tcaatttttt tgacgtttct
      301 atcatgaatc acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg
      361 aagttcttct tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt
      421 tttgtcgtat ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc
      481 tagatacaca aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact
      541 attgattagt tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa
      601 gaggaaacta acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca
      661 aatttggttt ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat
      721 acaccacaaa ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt
      781 ctacgtctcg tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac
      841 ctgcttcttg tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg
      901 cccaataatc aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc
      961 caatcaactg ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg
     1021 catacgccgc atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat
     1081 ttagataaga agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc
     1141 ctgcttgttt atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca
     1201 atgtccgaat aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt
     1261 ctttaacaac ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac
     1321 caagctcact atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca
     1381 aataaaacgg gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct
     1441 cgatttctgt catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa
     1501 cggttttacg atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat
     1561 cacgttctgt tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct
     1621 tttctacgac cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa
     1681 acaaatgtaa gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa
     1741 ttttatggct cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg
     1801 ctgctgtcga ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg
     1861 caatatcatt gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt
     1921 caatcgggcg attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc
     1981 ctttcccatc gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt
     2041 ggatcaccgt cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt
     2101 cactaaagtt catcacataa gggagcccat actcatctgt tcctaataga atcactttgg
     2161 ctaatgaagc cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt
     2221 acgcttgctg atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt
     2281 ccttgtattc tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc
     2341 ttttttcaat aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa
     2401 ggagggcaaa aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa
     2461 ataataagta acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta
     2521 atagcgtaaa acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct
     2581 catactcaaa gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg
     2641 ctaaaggaat actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga
     2701 taaaataaag ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat
     2761 cgacagaaaa catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga
     2821 tttcctccta tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt
     2881 ttcgtttcat ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg
     2941 gtgattttgt ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt
     3001 gtgctgttac tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag
     3061 atttcggtgt ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta
     3121 ccgcatgttt ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag
     3181 taagtgcctc cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt
     3241 ctgttctagg cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg
     3301 acaccaccat tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc
     3361 tttctcttag actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg
     3421 cctgtttctc cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg
     3481 cttgatcatg caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat
     3541 gacgaggttg ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt
     3601 gtggcatacg aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg
     3661 cgttttcttt ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt
     3721 gtttggcttc ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg
     3781 ctcctcgaac acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa
     3841 ttcccaacgg cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt
     3901 gtgtactact taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac
     3961 ctgtcattcc ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat
     4021 tcattgtgac aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata
     4081 gttgcatagt tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa
     4141 tcagattcac gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt
     4201 gtatgttctt gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct
     4261 cccctatttt tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc
     4321 cagcatattt gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca
     4381 acagatatgg ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg
     4441 tttccacttt tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt
     4501 cttcaggtgt taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag
     4561 aaaaaagaac atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag
     4621 taaaaataag gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag
     4681 catatttgat tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta
     4741 acatattgtc cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa
     4801 tatctaattc aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc
     4861 cactccaaac aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt
     4921 tcattgactc atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca
     4981 attgatccac atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt
     5041 cagaacgatc ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag
     5101 ccccttgtag acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt
     5161 tttttcgcaa aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg
     5221 cttgaatttt ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca
     5281 acacatcaat ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg
     5341 tttgtaattg ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca
     5401 atagtttctc tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact
     5461 cttttttatc ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat
     5521 tattcgcacg tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt
     5581 catacccttt tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga
     5641 tatacgtaaa cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta
     5701 atcgacccga atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt
     5761 tgatttgtga atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta
     5821 aaccaacgct atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt
     5881 cgataacttg acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt
     5941 ggatcaccgt tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc
     6001 ctaacacatt tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa
     6061 gcatccctcg acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag
     6121 ccacaaactc tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt
     6181 catttttcgg atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat
     6241 attttacaag gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg
     6301 tatgttccgc gccttcaata cttttctttg ttaaattggg ataataccat actagtttac
     6361 tagatagcgc taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta
     6421 aaggaaatcc tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa
     6481 aacctgtcgt agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt
     6541 gattaaacaa cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa
     6601 tcgcaaattc aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa
     6661 ccagcgtgac ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat
     6721 ccattaaccg ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg
     6781 tctcatattc tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat
     6841 cagattttga atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat
     6901 cctgaagatt ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta
     6961 aaaacgtcga ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg
     7021 aaatgacgcc ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg
     7081 atcgatggaa aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg
     7141 gcttataatt ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca
     7201 cactacttat aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct
     7261 taagcaattg aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa
     7321 acagatacgt tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt
     7381 caaagtacac tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt
     7441 caatgtgcgt atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat
     7501 catgtccaag tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta
     7561 aaggaaatac ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa
     7621 caaaatttcg gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct
     7681 ttttgaactg ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact
     7741 cgactcactc cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat
     7801 acatttcatg ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat
     7861 actcgaaaaa gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa
     7921 tcccaaaaat cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat
     7981 tcacatacgc aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta
     8041 attgttccat gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa
     8101 ttttgtgagg gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt
     8161 ttacaaaaaa agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt
     8221 ttcttatgat aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact
     8281 atccttaata aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa
     8341 taagacgagc attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc
     8401 ttgggtcgca taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac
     8461 ttgtccagat acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct
     8521 ctaagttcat tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact
     8581 tccattgaaa agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct
     8641 tgcaaaaagt agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat
     8701 tcgccgcctt ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca
     8761 tataactcaa aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc
     8821 gcttgcccag aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc
     8881 tcagaaagat tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg
     8941 actggaaaag aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa
     9001 aataggcgat acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct
     9061 ttcgctactc gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt
     9121 gctaaataat gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat
     9181 aacctttcaa taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg
     9241 ttgacttcag ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat
     9301 gtatccactc gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa
     9361 aaagcgcctg aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac
     9421 ggttctgttt tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt
     9481 ttcggtggct tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc
     9541 ataccgttct aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac
     9601 acttgccccc tc
//
LOCUS       DANJJW010000102         9564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000102 DANJJW010000000
VERSION     DANJJW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            93..995
                     /gene="gnd"
                     /locus_tag="PWS82_002297"
     CDS             93..995
                     /gene="gnd"
                     /locus_tag="PWS82_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL0907386.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            997..1998
                     /locus_tag="PWS82_002298"
     CDS             997..1998
                     /locus_tag="PWS82_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL0907387.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1995..2279
                     /locus_tag="PWS82_002299"
     CDS             1995..2279
                     /locus_tag="PWS82_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL0907388.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2312..3109
                     /locus_tag="PWS82_002300"
     CDS             2312..3109
                     /locus_tag="PWS82_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0907389.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3123..3956
                     /locus_tag="PWS82_002301"
     CDS             3123..3956
                     /locus_tag="PWS82_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907390.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3974..4831
                     /locus_tag="PWS82_002302"
     CDS             3974..4831
                     /locus_tag="PWS82_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0907391.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4853..5362
                     /locus_tag="PWS82_002303"
     CDS             4853..5362
                     /locus_tag="PWS82_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0907392.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5399..5800
                     /locus_tag="PWS82_002304"
     CDS             5399..5800
                     /locus_tag="PWS82_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907393.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5912..6265
                     /locus_tag="PWS82_002305"
     CDS             5912..6265
                     /locus_tag="PWS82_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907394.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6299..6742
                     /locus_tag="PWS82_002306"
     CDS             6299..6742
                     /locus_tag="PWS82_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0907395.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6759..7844
                     /gene="uxuA"
                     /locus_tag="PWS82_002307"
     CDS             6759..7844
                     /gene="uxuA"
                     /locus_tag="PWS82_002307"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0907396.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7837..8550
                     /locus_tag="PWS82_002308"
     CDS             7837..8550
                     /locus_tag="PWS82_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0907397.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8757..8906)
                     /locus_tag="PWS82_002309"
     CDS             complement(8757..8906)
                     /locus_tag="PWS82_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907398.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9302..9502
                     /locus_tag="PWS82_002310"
     CDS             9302..9502
                     /locus_tag="PWS82_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0907399.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3221 a         1500 c         2118 g         2725 t
ORIGIN      
        1 taggaaaaaa tcaaacaaaa aatatttttg taaaatctat ttacaaaaaa atttttttgt
       61 ataatgaggg tataagaaat ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa
      121 aaatgggatt gaatttagct ttaaacttaa aagagcacca tcacaaagta caaggactcg
      181 acatttcaag tacagctgtt agtcaggcaa gagaaaatga cattcctgct tatcaagaga
      241 tctctgaatt tctagctact tttgagcaga aaaaaatcat ctggttaatg cttcctgctg
      301 gagagataac cgaaaacatg ttaagaaaac tttttccaaa actcggacca ggagacatta
      361 ttattgaagg cggaaactct tattataaag attcaatccg tcgagcagaa gagtttcaga
      421 aaaatgaaat cggttacttg gattgtggga cttctggagg aatcgaagga gcacgacatc
      481 aagcctgctt aatgattgga ggagataaag aaacttttct atccgtggaa caattattca
      541 aagatgtagc aattgaaaga ggatatctgt atgctggtgc atccggaagc ggacacttct
      601 tgaaaatgat ccataatgga atcgaatatg gaatgatgca agcaatcggt gaaggattcc
      661 aacttgtaca aaaaagtgag tatgcttttg acttggaaca agtcgcacgt gtttggaatc
      721 atggttcggt gattcgcggc tggctgatgg aaatcatgga agatcagtta aacgaacatc
      781 ccaatttaag taattatcgg ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg
      841 aaacagcgct agaaatggct gttccagttc cagtaattgc aactagtttg ttcatgagga
      901 atctttcaca ggaagaagat agtttttcag caaaagtagt ttcagcacta agaaatgggt
      961 ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc cagattatca tatagcaatc
     1021 gttaattcca gcagtttcgg aagagtcttt ccacaccact tggaaagatt aaagtcagtt
     1081 ggagacgttc atttctttac tgttgatcaa gagattccag gaaaagaact agcggagtta
     1141 cttaaaggat tcaatatcat tattgcaagt gttacgccat tttttcgacg agattttttt
     1201 gaaaacaaag atgaactgct attgatttcc cgacatggta ttggttacaa caatattgat
     1261 ttaaaagcag caaaagaaca ccagacaatc gtatctatca tacctgcttt agttgaacga
     1321 aatgctgtag cagaaaataa tataactaat ttgttgtcca tgcttcgacg cacgacacaa
     1381 gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg cggagttcgt cggtaggacg
     1441 ctgtttaaca aaacagttgg tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa
     1501 attttgcgta acggttttcg ctgtgatgtt ctagcttatg atccttacaa atcagattta
     1561 tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag aattactgga acaatcagat
     1621 atcatttgtt tatgtgcgaa tttgacagaa gagaactatc acatgatttc tgataaagaa
     1681 gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg caagaggcgc tctagttgat
     1741 gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg caggatatgc cacagatgtt
     1801 ttagaagaag aaccgggacg tcaagatcat ccgtatttag cgtttgaaca agtagtgatt
     1861 acgccacata tatcggccta cacaagggaa tgtttagaag caatgggtga aaaatgtgtc
     1921 agagatgtcg aacagatcag tcagcacctt cttcctgaac gttctgtcca gacaaagagc
     1981 aactatataa aaggatgatg aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc
     2041 aattttattt atatgaagaa ggtggtattt caaaagtttc tgatctcttg aaatcatacg
     2101 gcgcgaaaag agtactggtc acccacggaa cggtatcatg ggaaaaggcg ctacctaaat
     2161 tagtattttt gaatgacgaa acgattcaat ttttttatca tcgttattct ggagaatgta
     2221 gttatgctga agccagacga atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt
     2281 tggcgttggc ggaggaaaac tgaccgatct agtgggttat agtgccaatc ttgcaagaat
     2341 cgatttcgga gtcatcccta ctttagctag taattgtgct ccatggacac ctcttgctgt
     2401 catgtataag gagaatggac aagcagaagg gaaaacagaa cacttttaca gacaagctgc
     2461 tttttttata acagatcccc aattagtgat tgattcccct gttccatatt tcattgccgg
     2521 gttagcagat acactagcaa aatggtatga atctgagctc atcttagaac aagatttttt
     2581 gcaagatgaa tcatttttga agctagcaca agatactgcg aagatctgca aagaagagat
     2641 tttgaatcat tctgctaaag caatccaaga tatggcacaa agaaaactga catcggagtt
     2701 caaacagctt tcagagatta tctttgctgt tgctggttta gttggaggat taggagataa
     2761 atacgcaaga aatgcggcag cacatgctat gcatgacgct atcagtaaat atattccgga
     2821 aagccatcgt tttctacatg gagaaaaagt agcgtatggg attttctatc aactggcact
     2881 agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg tatcgggaat tagaactgcc
     2941 ggtttctcta gaacaaatgg gattatttcc gaaagataaa cagataatag ataaaatggc
     3001 tgaatttatt gattcgaaag agaaagtgca tttgattcct atagaggtaa cacagaaatc
     3061 actgatacag tcaatgatgg aactggaagg ttatatcaga aagatctgaa ggaggaggac
     3121 acatggaaac attaagtgta gtacaaagtt tgttgattgc tttatgggta gccgcggtta
     3181 tgtcccgctg gttaggtgga ggagccacgt tgaccttacg tttttctcct ttgatgactg
     3241 gattagtcgt cgggttgatc atgggggaag ttgcacaagc gatgattgtt acagccgctt
     3301 tgcaaatgat ttatatgggg ggtttttctc caggaggatc aatgccagct gaaccatcta
     3361 ttgcagcagc tatcgcagta ccagtcgcat tattagggga tctgtcgcca gaagcagcga
     3421 ttgccgttgc ggtgccagtc ggattgctcg gaagttactt gtatcagttt cgttttttca
     3481 tcaatacttt tttaggtaaa tatacagaca aagctgtaga agatttgaat acaaaagcta
     3541 tggcccgatc aattattctt tatccaacgg ttgcctcatt tttattattc gttccgcttg
     3601 ttttcattgc tttatattta ggggcgccat tgatcgctga tgtaatcaaa gctttagagg
     3661 gaacagtcgt tatccacatt cttgaagtag taggtggagg gttagcggca atcggtatcg
     3721 cgacaactgt atacgttatc ggtcgaaaag attatctggt ctttttcttc cttgcttatt
     3781 ttatgagtgt tgttttgaaa tctttagata ttacgatggt tacttatgcc atttttggga
     3841 ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa acaagtacag cctgccacaa
     3901 gcaataacaa ttcagcatct tttgatgacg aagatgacta tgatgacgga ttttaattta
     3961 ggaggaatgg gaaatggctg aaataatgga taatcaaaca ggtgctcctg aagaaatcac
     4021 gaaaaaagat gtaacaaaag cctatctgag atggcatttt gcaaacgaaa ttcctcactc
     4081 ttttgaacgt tacttggcgc catcactact atacggaatg atgccaatct tgaaaaaact
     4141 ttataaagat gaagactctt taaaagcagc atataaacgg cagttactgt tcttcaacac
     4201 tcaattaagc tggggtggcg gtgtgattac tggcctaatg tcttctatgg aacagcaaag
     4261 agcagaagaa gagtatgaag aaaaagagat cttgatgcag gatgacttga tgtataacac
     4321 gaaagcggga ttgatggggg cactagcagg aatcggagat gcaatcgatt caggaaccgt
     4381 ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc
     4441 actttttcct tttatctgct ttgggttgta tcaggtttta ctaggggtat ttttcgcaag
     4501 acaagctttc actatgggcc gtaatgctac aggcctaatg caaaacactg gtgttcagac
     4561 agcgattgaa atgctatcag ttttagggtt gtttatgatg ggggtgcttg cgggaaacta
     4621 tgtaaaagta gaatcttcat tgaaatttgc gatatccgga cgtgagtttg ttatccagga
     4681 aattcttgat caaatcgtgc cagggatatt acctttagct gtcgtaatgg gtgtgtactt
     4741 gttctatacg aaaaaaggat tgaaagtcac acaagcatta ttatggctga ctggaatatt
     4801 aattgtgcta gcaggaatcg gtattttata aaaagaaggg aacgtgaata agatgggtgt
     4861 agtcaattta gcgcgtgtgg atgagcgttt gatccatggg caagtaatgg taacgttatc
     4921 tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa gttgtagcaa aagataaatt
     4981 tatgagagat ctttacaaaa atgcaggaag ccgtacagga caaaaaacaa ttgtcattac
     5041 accagagaaa gctaaattct attgggatga atatcaattt aaagaataca attgtatttt
     5101 gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa cacggaatcc ccatggaaga
     5161 attgaatatt ggagggattg ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc
     5221 tgtttattta aataaatcag atgctgaaaa attgaaagaa atgaatgaac aatatggcgt
     5281 aaagaatatt tattttcaag ccactccatc tgcacctaaa acaggattaa aagatgtatt
     5341 gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat
     5401 gaagaagaac gagctgtttc gtgattggga atttcgttat cgctatattt accgtaagcg
     5461 ccgtacaaaa aagagtaaac agcgttttct ttcagcatta gtatcagata tttacagtat
     5521 gagaacagat gtgacagtca ttgcttacga tacgctagcc taccgatcaa aaaatattta
     5581 tgttggagat atcgaaaaag cagagaaagt tatctgtact tattacgata cacccgttca
     5641 tgcattagga agttatttca tgtttgattg gaaagaccaa aggaaaaaaa caatctactc
     5701 tatattattg tccttcattt tgttgttcag tttaggttgg tggggaatga tgatatataa
     5761 caaaaatcct catcacgtat tcgatctgct atccgtataa acaggtatca ctgtactagc
     5821 atttgggagc tactttttct tattgggaaa agcagctcgc ggatggccta gcagacaaac
     5881 ttttattcga aatacttcat ccatattaac gatgctagaa atgatccgta cgattgatga
     5941 tccgagtgta gcgtatgcgt ttgttgatga aggctgttat ggagaaaagg gcttagacag
     6001 cgtgagatcg ggtatgaaaa aagaggctat tcttttttat ctagactctg ttggggcaga
     6061 tacaccgcta cagttttcgg gaaattattt ttcaaataaa gaacagtggc tgaaacaagt
     6121 tgataagttg aaagaaaaaa acgtcaatta tatttttagt gcaagaaaaa agcaagcgca
     6181 gttcttctat ttgaccaaaa cagatttacg agggaaaaca tttaattggc aaaatgccaa
     6241 tcagattatt gctctttttc gataagtaaa aagtgcaaaa caaggagtga gaagactaat
     6301 gttggaaatc atgatcgtta cacatggtaa gttgagcgaa ggactgaaag atgcggcgga
     6361 agtaattgtc gggacatcga gccatattga aacaatgtgc ttagagtctg gggaggacat
     6421 ccaaacattc ggagagaagg tcaaagaaaa attaactatg atcaacacag cagaacccat
     6481 gctagtattt gttgatctaa ttagcgcaag tccgtacaat caagcgctct tggcagtcaa
     6541 tgaactgact gaagaaaaga aagcagccac ttatgtcata gggggcgtga atctgccaat
     6601 gttgttagaa gcaatcaatc atcagatgct tcacacaccg atagaagaag cggtggaaca
     6661 aattgtcaaa caaggaaaag ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa
     6721 attagatgag gacgattttt agaaggaagg ctgaaaagat gaagtgggga tttagatggt
     6781 atggtgaaaa aggggattcg atcccattaa aatatgttcg ccaaataccg ggaataaatg
     6841 ggataattgg aactttgctg actaaaatgc ctggagatgt ttgggagatt tcagaaattg
     6901 aggagttgaa agcatctgtt gagagccaag gactggaact gcttggaatt gaaagtgtag
     6961 cgatccacga tgcaatcaag gcagggacag atgaacgaga ttactatatt gataactata
     7021 tacgaacaat caaaaacttg tctcgatgtg gggttcgatt gatttgttat agcttcaaac
     7081 caatttttgg atgggcgaaa actgatttgt ttaatgaaaa caacgatgga agctattctc
     7141 ttttatacga tcagaaaatc gtcgatcaga tggagccagg agaaatgtat caattgatac
     7201 acagccagtc aaagggattc aaattgccgg gttgggaaga agaacggttg aaaaaattcg
     7261 ctcagctgga aaaactgtat gaaggaatga ccgaagaaga attatttgaa aatctgactt
     7321 attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc
     7381 cagatgatcc gccatgggaa ctttttggtt ttcctagagt gacgaaaaat cttggcgact
     7441 tgaagaaaat tctaaatgca gtagattctc catataatgg actaacgctg tgcacaggat
     7501 ctttaggggc aaatccggaa aacgatttag ttgaaatcat ccatgaggta ggttcgcgca
     7561 tccattttgt ccacttcaga aacgtggagt ttttaggaga aagaaagttc aaagaaacgg
     7621 cacatccctc tacagaaggc tcccttgata tgtatcatat tatgaaagcg ttggtgcaag
     7681 aagggttcga cggtcctata cgccctgatc atggtagaac cgtgtggggt gaagtggcta
     7741 tgcctggata tggcctgtat gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg
     7801 aggcgataag taaggaaaaa ccactgggag agcattatga ataaaatcaa gctcttcatc
     7861 tttgatatgg atggcctttt attagaaact ggacgtttag cttatcgtgc ttatgtaaaa
     7921 tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct attatttact aactggccaa
     7981 acagaaatgg cgataagaga acaaatgggc attttgtacg gagaagatgt tccatatatc
     8041 aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg tgaaagaaga taagagagta
     8101 tatacgaaaa aaggagcaga agaaatttta tcttttgcaa aagaaagagg aatccataca
     8161 atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta
     8221 tatgccttgt ttgatcaaat catttcaggg gatgatgtaa aaaaaggaaa gccggaacca
     8281 gagatctttt tgaaagcttg ttcaaaaatg aatattcctc caagtgaagc acttgtattt
     8341 gaagactcga ttgcagggat cgaagcagca aggcgagcgg gcattttgtc ttttctcatc
     8401 aaagatcatt tgatagggtt gcccgatcat aatgggaagc ataagttgaa agtagatgta
     8461 agtttatatg aatccaataa agcagactat gaattttatg acctgcatca agcaaaacga
     8521 tttttagaaa gtaaagggtt gtatctctaa taaagaggac tattttctag tcctctttat
     8581 ttattgtcct gcacgactga gggaacagat gaaaaagaag taatttttta ttttgaagta
     8641 tctaccttat ttgtagtgaa caatttagaa caaacacaaa acgcatctac tgttatctac
     8701 cagtagatgc gtttaaagag aagaacgaga atcgtccata tgagggtttc cactccttat
     8761 gcagattttt ttaattcttg cactgcttgt tcgatcgtaa cacctgtcgc tacatgattg
     8821 gtattttctt tgtcaaaaac gacaaataga ttcaaatctg tatccaaggt tactcgatat
     8881 tgtatatctt tgttatagaa tgtcatagcc attctccttt ctttaaatac ttgctgaaag
     8941 gcaacgaacg cgttgataac gaaattccat ttataagtat accaccaatt gaaaggtttt
     9001 catagttaag aaattttaaa gtcaaaagtt gcagataaaa gcaaataaac aagaaaacaa
     9061 aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt cgaagacaat agaagacaat
     9121 gaattttata tttcattgca ttatccttgt gttattagaa aaagcatgat atattaaatg
     9181 agtaatctat tttgaaacgt aatttgagcg atatatctac atagataaaa ctccttttgc
     9241 ctatcaaata tttattgcaa cgaaaaaacg tacattgata cgtatcagga ggaatttatt
     9301 tatgaataac ggtacagtaa aatggttcaa cgcagacaaa ggttttggct ttatcactgg
     9361 tgaagatgga aatgatgtat ttgcacattt ttcagctatc caaggtgacg gcttcaaaac
     9421 tttagaagaa ggccaagcag tgtcttacga tgttgaagat ggtcaacgtg gccctcaagc
     9481 aacaaacatt gttaaagctt aataaaaatt gacttttaac cgtcttttcg aaagaaaaga
     9541 cggttttttt gtacgaaaaa attc
//
LOCUS       DANJJW010000103         9364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000103 DANJJW010000000
VERSION     DANJJW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..414)
                     /locus_tag="PWS82_002311"
     CDS             complement(<1..414)
                     /locus_tag="PWS82_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907400.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTY"
     gene            complement(711..3092)
                     /locus_tag="PWS82_002312"
     CDS             complement(711..3092)
                     /locus_tag="PWS82_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0907401.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3082..3729)
                     /gene="recU"
                     /locus_tag="PWS82_002313"
     CDS             complement(3082..3729)
                     /gene="recU"
                     /locus_tag="PWS82_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL0907402.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3793..4332
                     /locus_tag="PWS82_002314"
     CDS             3793..4332
                     /locus_tag="PWS82_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL0907403.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4422..4841
                     /gene="gpsB"
                     /locus_tag="PWS82_002315"
     CDS             4422..4841
                     /gene="gpsB"
                     /locus_tag="PWS82_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL0907404.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4863..5230
                     /gene="rnpB"
                     /locus_tag="PWS82_002316"
     ncRNA           4863..5230
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWS82_002316"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5439..6605
                     /locus_tag="PWS82_002317"
     CDS             5439..6605
                     /locus_tag="PWS82_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL0907405.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6651..8147
                     /locus_tag="PWS82_002318"
     CDS             6651..8147
                     /locus_tag="PWS82_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL0907406.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8228..8911
                     /locus_tag="PWS82_002319"
     CDS             8228..8911
                     /locus_tag="PWS82_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0907407.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         3008 a         1649 c         1950 g         2757 t
ORIGIN      
        1 ataagtgacg tatctatcta aaggtgtaat aaaaatgata agtgataata aaaaaatacc
       61 atttataact aaacataaac taatggattt gggcggtttc aggctcatca gaaaaatata
      121 gattctggtg aacaacaacc ataaaaaagt gagaatcatt gcatgagagc ttctttccat
      181 aaattttcct ttaagagtag tgcttaaaag tacagaaaaa ataataaatg caaggatagg
      241 agcaattaaa gtaaatttat ttaataataa acaaaatttt tcagcttttt tattcttgga
      301 ataacgaggt attgttttcg ataaaaaaag atggtgcaga caaattatga taattaagca
      361 tactatcaga taactcaaaa tgaagccttc tttcaaaaaa ttattcaaaa ccatatattg
      421 tattttttat ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag
      481 tgtgtattta gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa
      541 agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat
      601 agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt
      661 tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt
      721 ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt
      781 gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat
      841 gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct
      901 tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc
      961 tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt
     1021 gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga
     1081 attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata
     1141 agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg tgtcattttt
     1201 ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata
     1261 tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat
     1321 gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca
     1381 gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt
     1441 tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat
     1501 tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag
     1561 gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat
     1621 tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa
     1681 ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt
     1741 aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca
     1801 tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct
     1861 ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct
     1921 aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact
     1981 ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag
     2041 acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc
     2101 gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg
     2161 atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct
     2221 tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt
     2281 tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa
     2341 acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt
     2401 aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac
     2461 aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt
     2521 ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc
     2581 tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct
     2641 tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc
     2701 ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc
     2761 ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg
     2821 ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg
     2881 ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa
     2941 aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac
     3001 tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt
     3061 cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt
     3121 tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat
     3181 ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg
     3241 ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga
     3301 aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat
     3361 ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc
     3421 aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa
     3481 gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat
     3541 aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc
     3601 attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt
     3661 ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg
     3721 caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa
     3781 aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa
     3841 ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa
     3901 gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc
     3961 gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct
     4021 cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa
     4081 aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac
     4141 caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt
     4201 ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag
     4261 aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat
     4321 tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag
     4381 gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata
     4441 gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg
     4501 aagttgatga attcttagac aatatcatca aagattatga agcttacagc aaagaactcc
     4561 ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc
     4621 aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca
     4681 attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg
     4741 atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag
     4801 acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg
     4861 cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag
     4921 gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt
     4981 aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag
     5041 tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac
     5101 ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa
     5161 ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg
     5221 gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag
     5281 aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca
     5341 aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga
     5401 tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca
     5461 acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc
     5521 ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga
     5581 tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat
     5641 tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt
     5701 ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata
     5761 gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt
     5821 atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag
     5881 ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac
     5941 gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta
     6001 ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta
     6061 cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt
     6121 ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag
     6181 ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat atcaatggac
     6241 ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct
     6301 ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta
     6361 atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa
     6421 tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag
     6481 cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat
     6541 tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg
     6601 aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa
     6661 aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg
     6721 attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca
     6781 gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc
     6841 gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag
     6901 taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag
     6961 ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg
     7021 attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga
     7081 gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg
     7141 tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta
     7201 cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa
     7261 aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg
     7321 gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa
     7381 tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg
     7441 ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg
     7501 aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa
     7561 gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat
     7621 tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg
     7681 ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg
     7741 aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa
     7801 aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg
     7861 ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg
     7921 cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag
     7981 acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta
     8041 gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga
     8101 tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca
     8161 aagatagctg aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt
     8221 ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga
     8281 tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt
     8341 ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac
     8401 gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct
     8461 tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat
     8521 gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca
     8581 agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg
     8641 cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa
     8701 agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct
     8761 gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat
     8821 gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc
     8881 agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc
     8941 aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc
     9001 gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt
     9061 ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt attcggaaga
     9121 ataggactgt gacaagactt ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt
     9181 taagaaatat tcagagagta aaattaaaaa ttgctgataa acgaagatgg aaattttatg
     9241 tatttttatt ggtctatgca ataaataaat taaatattca gaatacaatt gattttaacg
     9301 aaagggcttc tatcaatggt tgtgttgtat ttatctgccc aggtagagta aaatagatca
     9361 ttat
//
LOCUS       DANJJW010000104         9362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000104 DANJJW010000000
VERSION     DANJJW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(93..761)
                     /locus_tag="PWS82_002320"
     CDS             complement(93..761)
                     /locus_tag="PWS82_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0907408.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="PWS82_002321"
     CDS             895..1182
                     /locus_tag="PWS82_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907409.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="PWS82_002322"
     CDS             1598..3136
                     /locus_tag="PWS82_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL0907410.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="PWS82_002323"
     CDS             3133..4098
                     /locus_tag="PWS82_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907411.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="PWS82_002324"
     CDS             4085..5407
                     /locus_tag="PWS82_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0907412.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="PWS82_002325"
     CDS             5648..6391
                     /locus_tag="PWS82_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907413.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="PWS82_002326"
     CDS             6406..7398
                     /locus_tag="PWS82_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0907414.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="PWS82_002327"
     CDS             7410..8147
                     /locus_tag="PWS82_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907415.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="PWS82_002328"
     CDS             8137..8859
                     /locus_tag="PWS82_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0907416.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="PWS82_002329"
     CDS             complement(8943..>9362)
                     /locus_tag="PWS82_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL0907417.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DANJJW010000105         9161 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000105 DANJJW010000000
VERSION     DANJJW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1872
                     /locus_tag="PWS82_002330"
     CDS             <1..1872
                     /locus_tag="PWS82_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0907418.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="PWS82_002331"
     CDS             complement(3807..4472)
                     /locus_tag="PWS82_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907419.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4652..6634
                     /locus_tag="PWS82_002332"
     CDS             4652..6634
                     /locus_tag="PWS82_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL0907420.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="PWS82_002333"
     CDS             6638..7276
                     /locus_tag="PWS82_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907421.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="PWS82_002334"
     CDS             7304..7732
                     /locus_tag="PWS82_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907422.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="PWS82_002335"
     CDS             8189..9100
                     /locus_tag="PWS82_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0907423.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         3189 a         1400 c         1723 g         2849 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     9121 gaactattta aatcaataaa actgttcatc taaatctttt t
//
LOCUS       DANJJW010000106         8953 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000106 DANJJW010000000
VERSION     DANJJW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            165..1379
                     /locus_tag="PWS82_002336"
                     /pseudo
     CDS             165..1379
                     /locus_tag="PWS82_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1558..1887)
                     /locus_tag="PWS82_002337"
     CDS             complement(1558..1887)
                     /locus_tag="PWS82_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0907424.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1877..2149)
                     /locus_tag="PWS82_002338"
     CDS             complement(1877..2149)
                     /locus_tag="PWS82_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0907425.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2600..3403)
                     /locus_tag="PWS82_002339"
     CDS             complement(2600..3403)
                     /locus_tag="PWS82_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0907426.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3381..4505)
                     /locus_tag="PWS82_002340"
     CDS             complement(3381..4505)
                     /locus_tag="PWS82_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907427.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4521..5336)
                     /locus_tag="PWS82_002341"
     CDS             complement(4521..5336)
                     /locus_tag="PWS82_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0907428.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5326..6198)
                     /locus_tag="PWS82_002342"
     CDS             complement(5326..6198)
                     /locus_tag="PWS82_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0907429.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6283..7572)
                     /locus_tag="PWS82_002343"
     CDS             complement(6283..7572)
                     /locus_tag="PWS82_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0907430.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7800..8444)
                     /locus_tag="PWS82_002344"
                     /pseudo
     CDS             complement(7800..8444)
                     /locus_tag="PWS82_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2782 a         1899 c         1426 g         2846 t
ORIGIN      
        1 ctaaatttta ccaaaagtga gtctcttttt gagttgaaac gaagaaaaag tgttagtaga
       61 aaatcgcctt cgattttcca ccaacacaaa atattataga agcaaaatca attctacctt
      121 agatgttctt atatcactct atcagtccta tttgacaaag agccaagaaa aagacgttgg
      181 tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg
      241 tagatggaaa cgggaaagca attatagtga aaaatgggaa gaaggaaacg attgtccgtt
      301 ttgaacaata caatcatatg cctttggtta cgcgcctaaa aaagcaacgc tatacctgta
      361 aaaactgtcg tacccattgg acggctcaaa gttattttgt ccgactcagc cattcaatcg
      421 cagatcatgt tcgatataaa attgcttctt tactgactga aaaagtatct ttgtctttta
      481 ttgcgaaaag ttgtcaggta tctttgacta ccgttactcg tacattgaaa gagtttaaaa
      541 gctatttacc aaagccttct aagaagattc tccccagagt attgatggtt gatgaatttc
      601 gttcgcacgc ttccatagaa gataaaatga gctttatttg cgcagatggt gaaacaggaa
      661 acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggct
      721 gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct tacttccagc
      781 ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat attgtcaaac
      841 acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt cgtaaagcag
      901 gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta aaaaatcgtg
      961 caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca aaatacccat
     1021 ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct ctaaaggagg
     1081 cgtatccctt ttttcatgaa ttagtcgaag cctttcgaga caaagaccct gacttatttt
     1141 tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggag aagcttcaaa
     1201 accttctgac ctatgaagaa ggtatcacca gcgcaatgat ctatccttat tccaatggaa
     1261 aaattgaagc gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat
     1321 cattcgagaa catgagaatt agaatctttt tgatcaatca attaatcaat gtaagataac
     1381 aaaaaatctg aaccagaatg tggttcattc tgacccggat ttgattttgc acaactcatc
     1441 agtccttatt gacaaagagc caaaaatctg agccagaatg aggttcattc tgacccagat
     1501 ttgattttgt acaacccatc agtccttatt gacaaagagc ccttcttttt agtattatta
     1561 taattcttcc atgcgttctt ccagtcgctg taaatcgctt ggacttaatt ccccaatttt
     1621 tttccatttg acctttttgt tgtttttggg taattcgttt agagttccta cgtcaatcca
     1681 agattctttt tttagtcctg cctgtttcca atcccttatt tcaacgtatt tcagctttat
     1741 tttatcggat ttagtatgat attttgaagt aattgacaga aacgaaattg tgtccagtcc
     1801 attttttaga atatagacag gccggcgttt accgccctct gcagctgtga aagcaacgta
     1861 gacggttgct atatcattag tattcatctt cattgaacca ttcttcaatt ttttcgtcag
     1921 tgtctagttt ttggacaggc aatttttccg ctaatctttg aatattatgg attcgtttac
     1981 ttttttccga caattcaata gaaaatggga tttttccttg ctcaacaatt tgcttataaa
     2041 aaacggtgac ggctgttttg gggctcatgc ctagttcatc aataatgctt tctgcttcat
     2101 ttttcaattt ttcgtccata gaaatttgta acttagccat tttctccacc tctttctgac
     2161 aaacacatta tatcacggaa agagttcaaa tggaattaat acaactactt ttgaactcct
     2221 tgaaatgcag tgtatttatc taaatataag gaaaggatat agtgctaaat ggccttgata
     2281 gatagaaaag tttcaaaatc cgccacagcg ataattgaag cgaacactca acgacacaca
     2341 gaacaggaaa agactcctaa aagaaccaaa ttaactagga cgtgtctgaa aactccacaa
     2401 agatgtggta tgctctggta aaaaacgaat ggcacaacca cgaagaccga tatggttctt
     2461 ggaaaacttt ctccaccctt tttgtaattg gagggatagt ggtttattga tagcgatttt
     2521 ccaagttctt caagtggaac cagacattga gcaccacggc taaaaagtga atgaagtctt
     2581 tatttttaat caataatttt taatgattac cagtaattgg ttttttcatg cgccctattt
     2641 ctatttgatg tggagttaaa taatgctgat aagtaaaaat ttttccgtca attaaactgc
     2701 cactagtgaa tcctgttcta ttggtaaaaa gagaatagtc tgggtattca tctacaccaa
     2761 atgataaaga ttcagccata aactgttttt taccagcaaa ttcagcactg ctaccttgat
     2821 ggatatgtcc tacaaaaata cctaaaatat cactttcggc aatcaattca gcaaaacctt
     2881 ccggaacttc agttacaata gatggatctt cccacgacaa tggatgatga accaataaaa
     2941 tcgttccctt ttcagcaggt gtacccaacc actcttgtaa ttgaatcatc tgtcctatcg
     3001 atatttttcc ttcgtgctgg gtatcttgag cagaatccaa ggaaataatt cgtaacccat
     3061 tttgattcgt acaatggata tactctcccg tttcattgct ttcaacactc attccttcaa
     3121 agaaaacact gcgacgatca tgattcccac gacaaaaata gtaaggtgtg gttgggaaat
     3181 attgatccaa cattttccgt aagacttgat aatcagaaat ccccccttca tgtactaaat
     3241 cacctgttaa tagaataaaa tcgtacactg ttaacgagag ttcttgtaac aatttttcca
     3301 atgccaactg tggattttta attgttggaa atagttcagc agtaaacaag ttagtttgat
     3361 agttttctct aaaatggata tcagagagat gcaaaaagcg catggctatt ccctctttct
     3421 tcaataaaat aatcaggata tcctaaattt tcttgcgtct gataatcaaa aaaatgcatt
     3481 ctttcaggtt ctatgcggag attgactgca tcaccaatct gccaagtagg tttttcatta
     3541 aggacaatca tttctatacc atcgacttta acggtgtacg cataactttg acctaatagc
     3601 tcacgatact tcacttgacc gtgtaaatcg cttgtttctt ttgaaaaggt tacgtgttct
     3661 ggtcttatgc ctaaatacaa tgagccacta acctgaagaa gttgtttttg ccaagctgga
     3721 ttcaatgtta aaaattgact ggctaccttt aatgttcctt gatcataagt aactggcata
     3781 atattcatgc ctggcgaacc aataaaattt gcagtaaaag tatttttagg tttcgtataa
     3841 acatttttcg gcgtatcaat catctgaatt ctaccttcat tcaataaaac aatccggtca
     3901 gctaacgtca tcgcttcaat ttgatcatga gtgacgtaga caaaagtttg tttataacgt
     3961 tgatgaattt ttaccaattc ttttcgtgca tccaatcgta aacgcacatc caaatttgat
     4021 aacggttcat ctaataaaaa ataattactc cgtttaacca ccgcacgagc caatgctaca
     4081 cgctgccgtt gccctcctga caaatcttta ggaagtcgat cttggtatgg cgttaaacct
     4141 aacatcttca aagcttcttc caaacggtgc tgaatgtcag ctgtcttaat tttattcgcc
     4201 cttaatgcaa aaataatatt ctcctttaca gtcatgtgag gatataaagc ataattttga
     4261 aaaaccattg cgatctctcg ttctccacaa ggtagcttat ttgctagttg tgtacctaaa
     4321 aaaagctctc cactcgtaat tttttccaaa ccagcaatca ttctcagcaa agtagatttt
     4381 cctgaaccag atggtcccag taacacaatt cgttctccag cggccacagc taaatcaatt
     4441 tcttttaaaa tggtatgttc tccatatttt tttccgatat ttcttaattc aagcgtttct
     4501 cccatgattt gtcctccaat ttatttaata cctgcagaaa caaaagtccc gataatatat
     4561 ttttgaaaaa ttgcatacaa gactaacggt acaaacattg taattaaact aatcgccatt
     4621 gctacggtaa aatttgtccc accttcagaa ctaatatagg tctgcatcgc caacggcaaa
     4681 gtataattat ctactgtccg taaaattaat accggccaaa catactcatt ccaagaggta
     4741 ataaaaaacc aaataccggt agaagtaatc gcatgtctag taagaggaaa aacaatttta
     4801 ctcatgacgg ttgtttgttt gatgttatcc aatgccgcca catctagaag gctcactgga
     4861 atttcccgca ttgtttgcgt caataaaaaa atgcccatcg catcagcaaa ttgtggtatt
     4921 atcacacccc aagtagtatc aattaaatta agttttgcaa tcaacaaata attaggaatc
     4981 atcgcaacag taaaaggaat gaacatcgta ccaatgatga taaagtacca gattttcttc
     5041 cctttgattc ggcaataagt aaaactatat gccgcaaaaa aagctacgcc taacttaaca
     5101 accgttataa ttgtagcaat aaaaaaagta tttctcgtaa ttttgataat tggaagattt
     5161 tcgaacaaat agcaaaaatt ttccaaggta aacggctgtg gtatcaaact agtaatcgtc
     5221 tgataggcat ctttcaatgg cttaaaagaa ttggccataa taaacaataa tggatagata
     5281 ataatgatta tgatgaacat aaataagaga tgtggcaacc agcgtttaat tttcatagta
     5341 aacccccttt tccacaaatt taaatattac ccctaagaaa acagcaaata ttaataaagt
     5401 taaaactgaa attgctgaag attctccgac tttatacatt tcaaaagcct tttgataagc
     5461 ataatagatc acgtttgcac taccattatt cggtccacca cgagtgatga tcttaatcgg
     5521 agtaaacaca tactgcagac cttgaacaat ggtcatcacc aataaatata gtcctgtcga
     5581 actactcata ggcaacacaa ttgtaaaaaa aatgcgccag atgggtacac gatccaactt
     5641 agctgcttcg atcatctctt gatcaattcc cacaattgct gcaaaaatga caatgaaatt
     5701 ataaccaaaa actttccaag cagtaatacc tgcaatcaca gcaattaccc aaccttgtaa
     5761 attcatccac ataggcagtc ttaaaccaaa atttcccaac aagacagcaa ttggtcccga
     5821 taccggattt aatatccatg taaaaaccat ccccgccact accaaagaaa tcacagctgg
     5881 aataaaaaat aatggtttat acaaattctg ccctttttct ataactattc ctaataaaaa
     5941 ggatgtaata tatgggaaca cacagctcag caagacaaat agaacggtat aggacaaagt
     6001 attcttcaac actttatgaa agactggatc agtcagcacc tttacatagt tggcaaagcc
     6061 aacaaattct ttagtggggg ccaccatatt ccagtcaaaa aaactcaaat agatcgtata
     6121 aatcaatgga taaaaaacaa acacgcccaa caaaataatt gctggcgcca gatacaaata
     6181 acctacatag cgcttcattt ccgacagcct cctcatcctt atgtcaaaag cagacgacct
     6241 tgtttttcct ttcttacagg cgctgtcgtc tgcttgcttt gcttatttga gtaactcatt
     6301 aatttccttt tgagtagctt tcatcgtttc attgacggaa ttacctgata aaattttatc
     6361 ccgcatatcc aacaattttt gttcagcttc taatccagca tctccaggaa atgaactcca
     6421 actgaccatc ttatccatct gatcaattgc tggtatcatc atctgatttt cagctaagaa
     6481 agcttttaat ccattttggg cgtctgcaac atctttacgt ggtggtaaat aaccagtacc
     6541 ttctgtccat ttagctaaag attcaacact ataaagatat tttaagaaat cccaagttgc
     6601 ttcctttttc ttatccgttt gtgatgtaac agctaacatt gcgccaccag caggtagttt
     6661 aacttttttc ccttcccaac ttggtgactc aactgcttta gcagtaaaag tactattttt
     6721 ttgaatgttg tccctttgag caatagtggt atagaccatc gcgacgttac catcaataaa
     6781 gctttgcaca ccttgttccc acgtagtgtg taatgccgtc tggtctttta ccatcctttg
     6841 atataattca taagcagcga taccatcttt agaagcaaag gtagctttgc catcttttat
     6901 tacacttgcc ccattacttt ctaaaattgc ttgttgtgcc caattatcag caggttcttg
     6961 aatatacacg ccatactttc cagttttttc ttttattatc ttagaaaaag tttgaacttc
     7021 ttcccaagtt tttggaccat tttcatccaa gcccgctgct tttaatatat cttgatttaa
     7081 atataaaacc ggattactaa tggagtaagg aatacccact tgtttaccat ctttttcaga
     7141 cagtgccatg atattgtcta aaaaatgatc tttaaaataa tcagcattat ccggatccac
     7201 ttcttctact aaagattgag gattagtata tttgaaattt tctgcaaaat aatctttaaa
     7261 agcccatccc acttgtacta cgtccggtat ttctttggag gcaacagaag cttgtaaatt
     7321 ttgcatcaag cctttataca tatcaggatt atattttgct acaaccttca ctttgtcttg
     7381 agattgattg tatttatcta ctaattcatt cactgttttt ccacctaggg tatccgcatt
     7441 aacatgccaa tactctatcg ttacacgacc ctccgatttt tttgttgtct tggtattccc
     7501 actaccacaa gctgccaaag agatgacact taaaacagac acaaccccta taacaagatt
     7561 tttctttaac ataacattaa cttcctccta atttctttat ggccattttt ctaatcaaga
     7621 aatattatga actaccaatg taaatttcta gttaattttc tgtaaataat tcgaaatttg
     7681 tgcatttcgg ggtacagggt cactggacat atccatcaag ctaagaataa agcccccata
     7741 tggtcatgta tcccatctcc aaatctctta actcctctgt tttgattata aaatagttta
     7801 ttctgtgctc ctttcttcca taaataggcc catgcacaac gtgaggttat attataaaca
     7861 ccacagcgcg caaggtactc aatcgtttct agttcattga ctgtccatac agaaaatgtc
     7921 aatcctagct gctgccccac ttcacgggcc aacttagtta accatccttc atgaatattg
     7981 acgatttgaa cgctttgata ggctaatttc tgtaaggtat cgatcaaatc tgcttcggta
     8041 atatccccaa gttttttccc ttcgaactga ttttcaatat tataaaatat atgctttccc
     8101 caaatcgcag cccaagataa ttgtacatag ccactaagaa caatttgtgg ccagatttga
     8161 taggtttgtg ccagcgaaaa aacggccatt tccaatccct cttcttttaa atcacagtta
     8221 atggtcatgg ttgccttctc attggccact gcttgaaaca cctgctcaag ggttaccgaa
     8281 tctgggacaa cttcttacct gtcatgggct agtattagcg taccatctgt ttgctgacgc
     8341 acatctactt caaaacaaag ctggcgatcc aaacaaaagc gaagaaatgc ccagctattc
     8401 tcctccgtat tgtcactacc agagtgtgca gtgatcatgg tcattttgac ccctccttta
     8461 attccgtgat tttctcccac acgtccacta taccgatgaa gcgttaattt cttgttaatt
     8521 tcttgtaaat tccctgcgtt ttttcagtaa actagttgta aatcttgcat gctccttcct
     8581 acgaactcat gaatcgtcga ttttgccctt ctagctagtt tcaccaatta agtagttcta
     8641 cattccccgc ttttaaccta ctacaccttt ttttgttttc agctattcct tcttcttgct
     8701 gaaagtcctt gaggttcttt cttacaggat accatcaagg tgttcaatgg cttattccca
     8761 ttgaatgacc gagacttact atcagatttc tgctcgttga accaagtctt tgatacagga
     8821 tttcttccgt catcttctca caaataccac cttgcgtttc tctagcgatt ggtcgatgtg
     8881 caccctccca gtggactgtc actacccctt tttgctatgc ctagcccaca aaaaaagctg
     8941 tcctcacaga cag
//
LOCUS       DANJJW010000107         8905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000107 DANJJW010000000
VERSION     DANJJW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(305..661)
                     /locus_tag="PWS82_002345"
     CDS             complement(305..661)
                     /locus_tag="PWS82_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL0907431.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="PWS82_002346"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="PWS82_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0907432.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="PWS82_002347"
     CDS             complement(1432..3618)
                     /locus_tag="PWS82_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0907433.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="PWS82_002348"
     CDS             3836..4519
                     /locus_tag="PWS82_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL0907434.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="PWS82_002349"
     CDS             complement(4600..5319)
                     /locus_tag="PWS82_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0907435.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="PWS82_002350"
     CDS             complement(5631..5837)
                     /locus_tag="PWS82_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907436.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="PWS82_002351"
     CDS             6082..6684
                     /locus_tag="PWS82_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0907437.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="PWS82_002352"
     CDS             6825..8105
                     /locus_tag="PWS82_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0907438.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="PWS82_002353"
     CDS             8118..8846
                     /locus_tag="PWS82_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0907439.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3188 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttctt
//
LOCUS       DANJJW010000108         8693 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000108 DANJJW010000000
VERSION     DANJJW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="PWS82_002354"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="PWS82_002354"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0907440.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWS82_002355"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWS82_002355"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL0907441.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4444)
                     /locus_tag="PWS82_002356"
     CDS             complement(3191..4444)
                     /locus_tag="PWS82_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0907442.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="PWS82_002357"
     CDS             complement(4450..5373)
                     /locus_tag="PWS82_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0907443.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="PWS82_002358"
     CDS             complement(5594..8086)
                     /locus_tag="PWS82_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0907444.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="PWS82_002359"
     CDS             complement(8093..8560)
                     /locus_tag="PWS82_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL0907445.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2392 a         1831 c         1446 g         3024 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DANJJW010000109         8691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000109 DANJJW010000000
VERSION     DANJJW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            123..515
                     /locus_tag="PWS82_002360"
     CDS             123..515
                     /locus_tag="PWS82_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907446.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="PWS82_002361"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="PWS82_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="PWS82_002362"
     CDS             857..1054
                     /locus_tag="PWS82_002362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907447.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="PWS82_002363"
     CDS             complement(1097..1672)
                     /locus_tag="PWS82_002363"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0907448.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="PWS82_002364"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="PWS82_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="PWS82_002365"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="PWS82_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL0907449.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="PWS82_002366"
     CDS             4334..5734
                     /locus_tag="PWS82_002366"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL0907450.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="PWS82_002367"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="PWS82_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7732..8325
                     /locus_tag="PWS82_002368"
                     /pseudo
     CDS             7732..8325
                     /locus_tag="PWS82_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8309..>8691)
                     /locus_tag="PWS82_002369"
     CDS             complement(8309..>8691)
                     /locus_tag="PWS82_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0907451.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2760 a         1447 c         1566 g         2918 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     7441 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     7501 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     7561 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     7621 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     7681 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     7741 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     7801 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     7861 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     7921 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     7981 ccggtaacag ttattgataa acccatataa gaacgtatgg cttcaacaat tcgacacaat
     8041 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     8101 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     8161 cgctttaagc aagtgtctgg tggaatggca acagtttttt tgacaaattt tataaggtgc
     8221 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
     8281 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
     8341 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
     8401 agccaagtgt accgtgcaac cgaaggtggt tccaccaatt tacatagtca aataactcca
     8461 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
     8521 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
     8581 tgatgtcaaa agttgttaat aattcatcaa tagcttggtt atcaaactct tttccacgat
     8641 cagtatgaaa aatctcaacc tctgtcagag gttgtttgat acggctaaat g
//
LOCUS       DANJJW010000110         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000110 DANJJW010000000
VERSION     DANJJW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..173)
                     /locus_tag="PWS82_002370"
     CDS             complement(<1..173)
                     /locus_tag="PWS82_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907452.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWS82_002371"
     CDS             complement(593..1714)
                     /locus_tag="PWS82_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL0907453.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWS82_002372"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWS82_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL0907454.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWS82_002373"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWS82_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL0907455.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWS82_002374"
     CDS             complement(4304..5215)
                     /locus_tag="PWS82_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907456.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWS82_002375"
     CDS             complement(5232..6239)
                     /locus_tag="PWS82_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0907457.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWS82_002376"
     CDS             complement(6236..8365)
                     /locus_tag="PWS82_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0907458.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWS82_002377"
     CDS             complement(8370..>8494)
                     /locus_tag="PWS82_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0907459.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANJJW010000111         8447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000111 DANJJW010000000
VERSION     DANJJW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..549
                     /locus_tag="PWS82_002378"
     CDS             <1..549
                     /locus_tag="PWS82_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0907460.1"
                     /translation="SLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNE
                     YLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQD
                     RELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKN
                     PNMFVNYQNDPYQFTTQVILLF"
     gene            564..1316
                     /locus_tag="PWS82_002379"
     CDS             564..1316
                     /locus_tag="PWS82_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0907461.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2019..2234)
                     /locus_tag="PWS82_002380"
     CDS             complement(2019..2234)
                     /locus_tag="PWS82_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL0907462.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2253..2489)
                     /locus_tag="PWS82_002381"
     CDS             complement(2253..2489)
                     /locus_tag="PWS82_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL0907463.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2747..4900
                     /locus_tag="PWS82_002382"
     CDS             2747..4900
                     /locus_tag="PWS82_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL0907464.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4869..6284
                     /locus_tag="PWS82_002383"
     CDS             4869..6284
                     /locus_tag="PWS82_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL0907465.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6317..7351
                     /gene="sppA"
                     /locus_tag="PWS82_002384"
     CDS             6317..7351
                     /gene="sppA"
                     /locus_tag="PWS82_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL0907466.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7353..7874
                     /locus_tag="PWS82_002385"
     CDS             7353..7874
                     /locus_tag="PWS82_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0907467.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7871..8257
                     /locus_tag="PWS82_002386"
     CDS             7871..8257
                     /locus_tag="PWS82_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0907468.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3025 a         1210 c         1514 g         2698 t
ORIGIN      
        1 agtttaaaag aaatcgctaa tcaaaaccat gattcgatat tggaaaaaga aataaaagaa
       61 ttagagatct attctaattc ttatctaacc aataagtttg aatatcgtta tttaaaaaat
      121 ataaaaaatg aatatttgaa aagtttgttg atagcgaagc tataccaagc aaataacaat
      181 aagatttgtt gtaaattcga atgtccaagt actattattg aagttcctgt tccaatattt
      241 gactgtatta gggtgcttgg aattattctc gataatgcaa ttgaagcatc tatagagagt
      301 caagatagag aactgtctct agctatttac cgagatgata aacagataga aatttcgata
      361 tcgaatagtt gtagagagat actactctct atagatactc taatgaaaaa aggaataaca
      421 acaaaaaagg gacatcaagg gttaggttta tctagtatag aggaaatcaa taaaaagaat
      481 cccaatatgt ttgtgaacta tcaaaacgat ccgtatcaat ttactactca agttattttg
      541 ttgttttgag ggaaggagga tttatgagtt atccaataat attatgtgaa gatcaaatta
      601 tacaattaaa tcagttagaa cgaattattg ataatttcat tctttttcac gataaagtat
      661 ttaagatagt attgaaaaca caaagtccgt tagaagttaa aaaatacctc aaacaattcc
      721 gaccaaagca aggaatatat ttcttagata ttgatttaaa tcatgaagtt aacggtatag
      781 aattagcaga agtaatcaga aaatatgatg ttcaagcaaa aatcattttt acaactactc
      841 atgatgagat gttacccgta acaataaaaa gaagagttga aacgttagga tttgtaacaa
      901 aagatcaaac actagatgag tatcgaaacg agattgttga gttattgtta ttagcgcaag
      961 aaaggataga tgcaacaaaa caggttacga atcaagcatt tgttttttcc ataggttctc
     1021 aaacatttac ttttgatata aatgacattt attttgtaga agcttcaaaa ttaccacata
     1081 gattatcatt gtgtactaaa gatgggcagt acgaatttta tggaagaatt agtgagcttg
     1141 aaaagaaata tcctatgtta acaagaatta gtcgagcgtg tttagtaaat atctttaacg
     1201 ttaaagaaat agattttaaa aaacgttcac tatattttga ttcagagttg gccagaaact
     1261 ttactttagg gaaagcacaa aaaattaagg agaagttgaa agaaagcact gtttagtggt
     1321 tttttcttta gggagattgc atttttctaa taaaatagat tccttcacta gtgcaaattt
     1381 ttctgaatcc tattaaagtt gtaccaattt ttatagctgt tattagttac gtgcataaca
     1441 gcaatattag ttactggagg aatatttcta tggtctggaa ccaaaaaagg gccaaatgaa
     1501 tagttctttt cttgttttta ttaagtacca taaaggggaa ttaaaattaa atgtatggtg
     1561 gaagatgcga aagatttggc aaattctact aatttttaga aagtgttttt cattttttag
     1621 ttaaaaaatt tgtcctgtca agtgcaatcc tatgattgta cttgatagga tttgagataa
     1681 ttactttcct gtttttataa atttaacttg caggaacagg ctttaatgca catccgacaa
     1741 gtacattacc aatacctcct gtaacggccg aaataggtcc tcctgcagca ccaactacag
     1801 cgctgcaagt ccttttgtaa aaatatctct accattacat ttccaaagaa tttttatctg
     1861 actttaataa aagataaaaa tttaatataa ttataaaaac tagcacaaga agctttagta
     1921 aaaagttctg tccattccaa acttttgaaa agataaagaa catacctatg ttcgtaatcg
     1981 acacaaacca atcttgaggt ttgatatttt ttaattgttt acgcaccggt ccacatgctg
     2041 aatccagcac ctaataaagc tccacctaca ccacaagcag ctattgcata aggtcctccg
     2101 gctgaacaaa gttttactcc tgctgttgcc ccagaaaaag tagtgcctaa aactcccacg
     2161 gcactcccac cttgaatatt tttcaactct tctgaagata atataaatgt tgtttttgtg
     2221 tttttaatat gcatactact tattctcctt ttttattttt ttcgagcatc ccagccagct
     2281 gcagcacctc cagcgacagc tccatataac cctccaacaa caccgcaagc tggtccgcaa
     2341 attgctgttc ctaaacctgc accacctact gcccctgtgg taagtccacc tacaacttgc
     2401 cccaattttc cccaagcatt attgcctcca ccaacagttt gtgcatttcc acaatactta
     2461 gttcctttac attttgcatg tttaaacacg cttctttaaa taatattttt tacggtgcta
     2521 ctgtaacata cgtatttgat taacagtaaa aatcttcctg aaacttatca agtttattct
     2581 tgaatagtaa aatttccaaa ataaatttga ttcaggaatg aaaaagttag tgttcaagaa
     2641 ctgttttcaa agtttttgtg gaattatgag acaatatttt cactgtatta aagctatatt
     2701 gagaattttt ctataaaaat tttttagaag gagttgtgtt agataagtga taaaatcatt
     2761 tccttttgtg cagcaacaag atgaaaaaga ttgtggtgtt gcttgccttt cgatgatttt
     2821 aaaatatttt aaaactgata tcccgattca taaattacgg gacctatctg ggactgattt
     2881 agagggtact tcagcatttg gattgaaaaa gacatttgaa aaattgtctt ttgattgtat
     2941 ggctattcaa gcagatagtg agatctggaa agaaagagat cttatcttac cattaattgc
     3001 acatatattg gttgataaca gctatatgca ctacgttgta atttataaaa cgaagggaaa
     3061 cgtactatgg attgcagatc ccgctaatgg aaaaaataga aaaacgattg aagcgtttca
     3121 aaaagaatgg acaggtattc ttttattacc tatgcctaga gaaacttata ttcctattaa
     3181 agaaaaagta gctggattaa gttctttttt gcctataatt tggaaacaaa aaggattagt
     3241 attccacatc gttttagcat cgattttcat tacgtttttt ggcataggaa gctcatatta
     3301 ttttcaaggt attcttgatt ttttcatccc taatcaagca cgttcaactt taaatataat
     3361 ttccattggc ttgatcgctg tctatttatt tcgggtattg tttgaatata gtcgtagtta
     3421 tcttcttatt attttaggac agcgaatgag tatggcaatt atgcttcagt atttcaaaca
     3481 tgtgttaact ttaccaatga atttttttgc tacgagaaaa tcaggggaaa ttatttctcg
     3541 ttttcttgat gccagtaaga ttattgatgc tttagcaagt gctacgttat caatattttt
     3601 agatattggt atggtgttgc ttgttggggt tacgttagct attcagaata gtacgttgtt
     3661 tttaatcact ctatgctctt tgccttttta tgtagttgca atattcgctt tcgtgaaaag
     3721 ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca gctgtaaatt ctagtatcat
     3781 agaaagtctt aaaggaatag aaacaattaa agcatatagc ggtgaagaga aagtatataa
     3841 ccgtatagac catgaatttg ttaaattaat gaaaaaatca tttcgtacgg ttacgcttga
     3901 taatattcaa caaggaatta aacatttgat tcaattaatt agcagtgcat taattttatg
     3961 gctaggttct tattttgtta tggataatag aattagtcta ggtcaactta tcacatacaa
     4021 tgctttacta gtatttttga cagatccttt acaaaacatt attaatttac aagtgaaaat
     4081 gcaaacggct caagttgcaa ataatcgttt aaatgaaatt ttcgcgattg attcagaaaa
     4141 atcgaaccag tcttttgaat atatgctttc tgaacaatta tttcaacaag atatcactgt
     4201 aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg ttgaacgaaa tttcatgtac
     4261 aattccttcg cacagtaaaa cggctatagt tggagttagt ggttctggaa aatcaactct
     4321 tgcaaagtta tttgttcgtt tttatgaagc atcagaagga tcgatccatt atggaaacat
     4381 taatcactta gacattcctt ttcagatatt acgcgatcga gtgacatatt tgcctcaaga
     4441 gtcatttttc tttagtggaa caatcattga gaatcttata ttcggtttag atagcccacc
     4501 aagtttcgaa caaataatag aagtttgtga gatagtccaa ctaaatgaat ttattaatca
     4561 acagccatta cgttacgaga cattgttgga agaaggtgga aataatcttt caggagggca
     4621 acgacaacga ttagccttag cacgggcttt attaaaaaat ccggacatat taattttaga
     4681 tgaagcaact agtggattag ataccttgtt agaacatgca attatgaatt atctcctcaa
     4741 tttaaaagaa aaaacgatta tttttattgc gcatcattta ccaatagcaa aagcatgtga
     4801 tcaaatttta gttctccatg aagggcattt ggtagaaaaa ggaacgcacg atgaacttcg
     4861 ttttgccgat ggtatctatc aaaaattatg ggaaatctag gagagggaaa caatttatgc
     4921 agaataataa ttggttcgaa cattctagta tctatactaa acaacatcat agcttctacc
     4981 gttgggtgtt ataccctgta attctttttt tatgtattat tggattgttt ttaacctttg
     5041 ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa ccccgaaaaa atagagaagt
     5101 tacaagtgcc attagaagct aaaataatgg aaaacttcct aaaagaaaat caatttgttc
     5161 ataaggacga tgtgttggtt agattggatt gttctttaat agagaatgaa aaggctcaga
     5221 tagaacaaga aaaccaacgt attactcaac aaataaagat ggctcagcta tttattgaaa
     5281 gtataagtaa aggaaaaaat ttgttttcaa cggatgacag ttttggctac agtaatcaat
     5341 taaagagcat gttgtcagaa aaagaatcac tccgctacgc tttgaagcaa agtgaattaa
     5401 atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag acaactagaa aaacaaattg
     5461 agagttcaga tagtaaatta caagaatggc aacaagtaca ggtagcttgg agtaataatc
     5521 aatcattaaa agatttttca aaagaaatga tggcaaacta tgagaattgg caagaacaac
     5581 taaataatgt ttctgatgat caaaaaaatc aagtgaaact gacaatttca gcaagcataa
     5641 atgaacaaat tgagcaacta aaaaaagaag tagaacagta tcagtcagaa aaagctaaat
     5701 tagttaaacc aactacttct gagaatgaca gaattagtca aacggaaaaa ggaaagcaag
     5761 agctagaaca agcaataaca actgtaaaac aaacagttac agagctaaat gaaaaacaag
     5821 agaagaacca gtcagttatc aaaagtttgg acgatcaact ttcaaaagga atactaaaag
     5881 ctcctgtatc tggaacggtt catttaaatg aagaaacaaa gggacaagca gaactagcca
     5941 aaggaacagt gcttgcagaa atttatccga tccacgaaaa atcaaaaatg aaatttacag
     6001 cacttttacc tgcgaatgaa agtacctata ttaaagaagg gatgaaggtt cattttaaat
     6061 tggatcaaaa aggtggggct ccgattacga tcgacggaac tttagatgaa atttcagaaa
     6121 attctactgc cactgagaaa ggtgtttttt atgcagtgaa aggaacgtta aaaacaactc
     6181 aatcgtttcc gtttcgttat ggattaactg gagagttgtc tttaattgta ggagaaaaaa
     6241 catattggca acaaattgtc gatgtgtttc taaataaaaa ataattattt ttactaagaa
     6301 tataggagga gtttatatga ataaaagaag atggattgct gtgggagtag cgtgcggatt
     6361 attgttacta tccattatcg tcgcgctaat tccaggaaaa gataaagagg aagcatcaaa
     6421 tacaacccta acaggaataa ataagatttt ttacggatct aacgaaatca cagaagaaac
     6481 gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta tccgtcaatg gagttattgc
     6541 agatacaggt gaaagcaatc tttttagtag ggaacaatat aaccatcaga attttttaac
     6601 acagctaaag aaaattcaag aagacaaagc ggttaaggga gtgttattag aagtaaattc
     6661 acctggggga ggaatatatg aaagtgctga gattgctaaa gaaatggcaa aaattaaaaa
     6721 gctagatatt cctatctata ctgcatttaa aaatacagcg gcaagtggag ggtactacat
     6781 ttctgctggt tcagataaaa tatttgctac ggaagaaaca acaactggat caattggtgt
     6841 cattatttct ggtttaaatt attctggctt attagaaaaa cttggcgtaa ctgatgcgac
     6901 gtataaaagt ggtgcattaa aagatatgat gtcacctcaa cacaaacctt cagaggaaga
     6961 gaataaagtt atacaggaat ttgtgatgag tgcttatgat cgttttgtca atgttgtagc
     7021 aaaaggaaga aatatggata ctaatgcagt gaaagaactt gcggatggaa gaatttatga
     7081 tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt ggttactctg aagatgcatt
     7141 agattcccta aaaaaagaga agaaattaac tgatgcgacg gttatagaat ataaaaatga
     7201 cacaactggc tttgcttctt cttggttagg taataagatt gctgaatggc aagggctaaa
     7261 ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa cttggatttt ctgatactcc
     7321 aaaaccaatg tattactatg gagggctata aaatgaataa gaagagtggc attgaaactt
     7381 tgcctaatta tgtatttgcg ggattttggt tacgattttt tgcttttctt attgatttaa
     7441 tttgtattag catcatcacc catgctacta taggtctctt ttacaaaatc atgggactgg
     7501 aatctgacaa atccattatt actttttatg gtatagcgtc attagctatt tatttaacct
     7561 attttgtact tttgactaag ctgaattcag gacaaacaat tgggaaaatg atttttggga
     7621 taagagtagt gagctttgag gaaaaagagc taagttggca aactatcttt attcgtgagt
     7681 gtatatgtag gtttatatta aaaaattttg tatttcaaat tgggtatttg gtggctgctt
     7741 tcacgcccaa gaaacaacat attggggatt atttttctaa cacgtcagtg gttgttattc
     7801 gttcattaga agctattaac tatggtcaaa aaatttctaa ttctaaaaca cttaatctgg
     7861 agagaattaa atgaataaga gaaaaacaga gtttattttg attttaatta gtggaataac
     7921 agggatttta tgtagtcttt tagcattgat gggtggtatg tttctatctt ctattgattc
     7981 aactacttta aatactacag aactaaccag taaggatata gatatgttag tcaatctaag
     8041 cgggaatgtt gttttatttg gaggaattac cctagttttt tcgataatga ttttcataat
     8101 aggtttttta gtaagaaaaa atcaaaaaac agtggtctat ggtattagtt tactcatttt
     8161 atctattatt ccattttttc ttttaacatt attgtgggta attcctggag ttttaggtat
     8221 tattgcggga ttgatgcttc taataagaaa atcttaatta ggcaatgtgt agacaacgtg
     8281 aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa tgataacttg tagtcgatgg
     8341 aagacatgta gataaaaaaa ctacgtgtct tttatttatt ctaatttttt cgaaaaaaag
     8401 acactctctg attgttgtat taatggaaaa ggggaaagca gcaagta
//
LOCUS       DANJJW010000112         8346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000112 DANJJW010000000
VERSION     DANJJW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            90..563
                     /locus_tag="PWS82_002387"
     CDS             90..563
                     /locus_tag="PWS82_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL0907469.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            572..799
                     /locus_tag="PWS82_002388"
     CDS             572..799
                     /locus_tag="PWS82_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0907470.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1435..1806
                     /locus_tag="PWS82_002389"
     CDS             1435..1806
                     /locus_tag="PWS82_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907471.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2062..2304)
                     /locus_tag="PWS82_002390"
     CDS             complement(2062..2304)
                     /locus_tag="PWS82_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0907472.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2336..3238)
                     /locus_tag="PWS82_002391"
     CDS             complement(2336..3238)
                     /locus_tag="PWS82_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0907473.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3251..3439)
                     /locus_tag="PWS82_002392"
     CDS             complement(3251..3439)
                     /locus_tag="PWS82_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0907474.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3453..4016)
                     /gene="amaP"
                     /locus_tag="PWS82_002393"
     CDS             complement(3453..4016)
                     /gene="amaP"
                     /locus_tag="PWS82_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0907475.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4054..4947)
                     /locus_tag="PWS82_002394"
     CDS             complement(4054..4947)
                     /locus_tag="PWS82_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0907476.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5025..5948)
                     /locus_tag="PWS82_002395"
     CDS             complement(5025..5948)
                     /locus_tag="PWS82_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0907477.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5997..6347)
                     /locus_tag="PWS82_002396"
     CDS             complement(5997..6347)
                     /locus_tag="PWS82_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0907478.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6380..7282)
                     /locus_tag="PWS82_002397"
     CDS             complement(6380..7282)
                     /locus_tag="PWS82_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0907479.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7275..8132)
                     /locus_tag="PWS82_002398"
     CDS             complement(7275..8132)
                     /locus_tag="PWS82_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0907480.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2295 a         1602 c         1405 g         3044 t
ORIGIN      
        1 atatttttat tgtttttcga taaaaaatag ttgttttaca ataattattc tgctatattt
       61 tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg
      121 tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg
      181 ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt
      241 actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa
      301 tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa
      361 ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg
      421 atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg
      481 cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga
      541 cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt
      601 attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc
      661 aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa
      721 aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa
      781 tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg
      841 atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat
      901 tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg ggccttttct
      961 tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc
     1021 tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa
     1081 taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct
     1141 aatggcccat tgtgttaact cttactccat tcttttttag taagctccca tatatactgt
     1201 aaagccttct atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa
     1261 atgatttgaa taacagtgat ggacaattct tttttatttt taacattatt ttgataataa
     1321 tgattattta tttaaaaata cattagataa catttgttac gacacgaaaa agcttcacaa
     1381 acaaatatcg ttattttaaa atgagggcga actcacacaa ttaggaggaa tagtatgcca
     1441 tttattgttt acttttttct ttcattactc gtgatctata ttccattacc aacatttttc
     1501 ctgctcgtca atcgtctagc agagcaacat gatacgattg ctgctcttac gaaactttgt
     1561 ttacttctat ttgtttgtct agattacttt acgagctatt attggctaaa acagacaaga
     1621 gtaaaaaaga gatattattt cttccttgtc tttttcaatc tctttgagtt tctgatccat
     1681 tgctttttac ttcatcagta tcatacgatc catttgtgga tcatctgggt tttcaaagtg
     1741 gtcgtattat tttgtatgtt cacctttcct atctcaagaa aatttaggga ggcattgttt
     1801 tattaaagaa gcttctctgt atcaataaag acacatttaa ggaaaaactt ttaaaaatct
     1861 aattgggaat ttgcccgcat aaataagagg ctgtgacaaa agtcttgtca cagcctctta
     1921 ttccgtataa acagtgttca aaagcttgca tcttcgataa tatgcccaac caaatgaaaa
     1981 atatgagaag ctatttttgt ttggctgttc ttattacttg atgaagaaca gctttttcac
     2041 gacctctttt taaaagtctt tttacagttt tcgagcaaag aatgatacga ttagtactaa
     2101 aatgatggca ccaatcaatg atggcacgat tgccattcct gcaatacttg gtccccaaga
     2161 acctaataat gcttgaccaa gtgctgatcc aactaaacct gctacgacat ttgcaataat
     2221 ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt gctccgataa ttcctccgac
     2281 gataagtgac caaataaatc ccatgtttcg ttcctcctaa tgttttactt ccttattact
     2341 gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc gcttccagct gctgatttta
     2401 ctttatctgt ttgattcgaa gcaaattcac cggttttttt cgctgcgtca gttactttgt
     2461 cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt tttgatgtcc actacgttag
     2521 cgttgacttc gatcacttct aaatgtgtca tattttttac ttctttgccg attacttctt
     2581 gcatttcttc aaagattttt tcgatatctt tgccatattc cacaatgaca tccatatcaa
     2641 ctgctacttg ttttttaccg acttctgttt cgatcccagc cgttacatta tctgtattca
     2701 caagttttcc tgccacatta gagaagaaac caccgtttgc agataataaa ccgtctactt
     2761 gttcaattgc gattccgatg attttttgca caactttatc ttcataattc agctcaccgc
     2821 gcattgaatc ttcctgttca gaatcatgct ttccttggtt atttgtatga tcagaaccaa
     2881 gttttccttg tccgtttgtg tggtcagaac taagtttttc ttgtgctttc gcatggtcag
     2941 aaggatgttt ttgctcttcc acaggtttag aaggatcgat tgcatcatgt tgttctggaa
     3001 ctttagattc tggagttcct ggatgatgag gtgcatgctt ttctgcatca tgtttcttct
     3061 cttctactcc ttgttgaacg ttagggacta ctggatctgg atgtgcagat actggtgctg
     3121 ttttgttctt ttcatcgttc acaccatttg attggtttgg tgtcgttggg tctggatgtg
     3181 cagacactgg tttttggttg ttcattttat tgttattatt tttgttattg ttattcatgg
     3241 atgtttcctc ctaagattta aaatagtgat ctaagatttt cgtttgtttc agataaaaac
     3301 cgatagcgac gccgatcgct gccatgatca agatcaataa ggtcttgaaa aagcccacgg
     3361 tgatccataa aacggctaat atcaatccaa gtaccccaaa gatgataggg agcttgtatg
     3421 tcgaaaatag ttctttcatt tttggtcccc ccctattcta ctcgtttgcg gtcattgctt
     3481 cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata ctttcactgg ctcttttgtt
     3541 cccaatatgt gttgtacttc attttcgatt tctttcatca atgttcctgt tttaccaatg
     3601 agtgctgaag ttcgtttcaa ctcccctttt acattgacgg cgatcttgtt tttcgttgct
     3661 ttgacattta cttttggtga atggatgaat tcatcttcat gcaagtgaga acgaaccatt
     3721 ccttcaattg cttttttatc caacgtcaac tccccatcac catgtttcag tagaaagttt
     3781 ccgattgttt ttggataaaa caggaaaaca agtaacgtga taattgtcaa aatagcaaat
     3841 gcaacggcaa gccaaaatac atattgttgc acgtatgaat tagtgatgga aaagaatcga
     3901 aacttctcta gctgaaaagg caatggatag gttgcactta atagtatcgc aaacaaaagc
     3961 acagatagca aaagtaatgt tccgatcact ccgatcgttt ttacaccttt attcattttc
     4021 gcacctcctc ataggggtga aacgatcaac ttcttataag ttgatcgctt ctccaccagt
     4081 tactccgtaa atttgggctg taacataact tgctttattc gatgctaaga aaacataaac
     4141 tggtgctaat tctcctggct gacctgcacg ttctagtaaa ctttgctgac caaattcagg
     4201 gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt gtccagattg gaccaggtgc
     4261 tacaccattt acacggattc ctttaggtgc aagctgtttt gctaaaccga tcgtaaagtt
     4321 agcgattgaa gcttttgttg ctgcgtaatc taataaatgt tcgcttggat tgaatgcttg
     4381 aacagaagtt gttgttacga ttgctcctcc tgctgggata tgcggaacag cttctttcac
     4441 taaagcaaac atagcgatga tgtttatcat aaatgtgtct ttgacttgct caagtggtaa
     4501 ctcagcaata gactgacaag aaatttgctg tcctgcgttt agtaccatcg tatctagccc
     4561 gccaagttca ctcacggctt tatctatgat ttctttaggt gcttgttcgt cacgaagatc
     4621 aaaaggtagt aagacaatct tacgtcctgc atctttcacg tattttgcta cttcctctgc
     4681 atctttttct tctcctggga agaattgaag agcgatatct gccccttctc tagcaaatgc
     4741 aatcgctgct gcacgaccga tccctgaatc tccaccagtg attagtacac ggcggttttc
     4801 tagttgatta ttgccttcat aggattcttc tccacaatca ggaacaggaa tcatattctg
     4861 ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc tccgtatggt acaagtttcg
     4921 cgggtcgatt aattttggtt ctgtcattgc tgtattcctc ctagttttta aattgtattg
     4981 ccaaaacaaa tttctttgtt tgatacctgt tttaaataga ttctttaatt tgtataatct
     5041 ttccggtata aatggtaacc taaaactact gctgctatca aagagccaaa gactacgagt
     5101 ataaagccta gagcagaact tttacccggt aatcctcctg tattcattcc ccaaatccct
     5161 gcgaacaacg ccggaacgga aatgattaat gcagctgaat ctagatattt catgatatga
     5221 tttagattat tgtccatcat cccgttgaat agatcaccaa tactctctaa taaatctcga
     5281 tagatctcga tcattttttc tgtttgtcgc tgtctcaaat ggacatcata gagtagctgt
     5341 tcatcgtcta aacgtttgat gaaagtttca gaattccata attgatccat tgtttcccgt
     5401 tgatctctta aggtatgatc gatatatacg atgtctcttt caagatctgc ctgtttatac
     5461 agttcttcat tttccgttgt tttacgagct gctttatcca aagcgtcaat tcgctttttc
     5521 atctctctta gttctttcac gtaatgggtg taaatatcta ataatgtata cgtgatgatc
     5581 ttttcaaacg agtttaaatg atttccatgc tttgtcagca gccgatccac aaattcggtt
     5641 tttctatcga tacaagtaat caagagatta tcggaaagta caaaagatat tggcgttaat
     5701 tttttttcga tacgattatc tgactgatct agattaatca ctaccaaaga aaatggatgc
     5761 tctagtttcg tctcataaag gcgctccatc cgagaaactt cttccggata ttcggttcct
     5821 acgaaaatat catcaggaag ctcgaattct tcgattgctt gctcaatctc ttcgggcttt
     5881 gcactatcaa gtacgagcca attatatttt ttttcatctg tttctattac attcttttct
     5941 tcatccacaa agtagtatct caatgtatac ctcctttttg gactttctcg aaactattag
     6001 ctgaagaagc agttctttaa tttgatatta taatcgagta ctgcgatttc attttctaat
     6061 gaaaagctgt caagctgttt gttgaagtcc atgttcaatc gtttaccgaa ataattttgc
     6121 tctaatttag atgtatagat gtccaataat ggatgctcga ttttagtcgc atattctcca
     6181 aattccgaaa caaaaggaat gatttgaaaa aagctgcctt ttgcaaaagc acctttgtcc
     6241 cttagaaatg gatcggaatc attgataagc gcgagtgcga tttttgtatt ttccccaacc
     6301 ttttcaatta acgtctctac tgcattttta gacattgtaa tttccattcc tactccccct
     6361 cttcagttgt tgtcttctat tatttaacaa ctacatctgt ttttggttga acttttgcat
     6421 attttggata tttagggaat ttagctacac aacggaaagc cgtcatccct gcatattttt
     6481 cgtaatattt cttcgttggc ccttcaaaat ccttgctcca cggtttcaca tgtcctgaaa
     6541 aatggataat gtaaggattg ttgcgagttt cttcgtattc acgttcacct gctgctgttg
     6601 gatgtttttt tgcttttgcc atgatatagc cttgtgcatt ccaacgcgga tgaagtggta
     6661 accaacggtc atgtaagata gcatttaatg catcttggtc atggaatcgt aatttttctg
     6721 gattatgttc gatgaaatct aatacttttt gcgtaatatt ttcatctaac cattttttca
     6781 cattgatcag catcaaacct gagttgaaat aacgcattga tttagctgaa attttcattt
     6841 tttccagtcg ttgatggaac ccagcgtctt caacagctgc tacgattgaa tcaccgaagt
     6901 caagacgcca taatttagag atatcttgta aagcgatcat gtcgcaatcc atatataaga
     6961 tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc aatacgataa taagctgttt
     7021 ctggaatacg atcgctgatt accacatttg taaagaattc tttattgatt ttcaagaatt
     7081 ccacatctga gttcattcgt gcgtttttca ctgagaagcg taaaagctgt ttactttcaa
     7141 aatcgatatc atcgtcaatc acatagaacc gaacaaattt agacgggttg caattatcta
     7201 atactgagac aaataatgca gctaaatgtg gtacgaattt tgtgttacaa cttgcgacta
     7261 ctgctatttc ttttttattc attgatctcg cctactttcg ttagagtact gtgtgctaat
     7321 ttttttaagt ataaattggt atacggatga tctttcaacg tattccaagg tttatcatgt
     7381 cctgtaaagt gaacgatcga aggtttttca tttccttctt tatactgacg ttcatatttt
     7441 tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct gcatgttcca ttttggatga
     7501 agttgttccc aatcttcata aagtacagca ttcaaagcat cttggtcgtg atagatgatt
     7561 cgatcgccat tttcactgag gtaatggata gttttttctg taatttcttt tttattccat
     7621 tgatcaatgt caatcaccat cactcctgaa ttgaagtaca agtcatcgga atcgattcct
     7681 aatctttcca aagctttcac ttggcctgga tcgataatag cgccgattgt ttttccattt
     7741 aacggctcat catatagttt tacgatatcg tctagtacta acacatctga atctaagtaa
     7801 aggactttct tataatcctc tttagctaat aagtttggta aagaaattct taagtaagcg
     7861 gtagttgtga tatggtcact gactaggaag tcttcgtaga tatctttttc cactgtcagg
     7921 aattggatac ttgcattact tgaatattta tttaccgttt cttccaatcc ttctttacta
     7981 tattctgata gaccatcgtc aatcacataa aattttatgc gtcttgtttt attgcaattt
     8041 tctaatgcag ttgcgatcat cacgctcaag taaggagcat aattttcgtc tgaagcagtt
     8101 accacaggaa ctacgccgta tctcttctcc atcaaaatca ctcctttttc taatcttttt
     8161 ctaacctgtt ctacatctag aatattcctc atttggtttt aaagcaaaat atatgctttg
     8221 ttttcattga atattttaaa atttttttat cacaaaacgc atcggtaagc gctaactatt
     8281 cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa ctactatata tagccacctt
     8341 tttctt
//
LOCUS       DANJJW010000113         8188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000113 DANJJW010000000
VERSION     DANJJW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            16..546
                     /gene="lepB"
                     /locus_tag="PWS82_002399"
     CDS             16..546
                     /gene="lepB"
                     /locus_tag="PWS82_002399"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0907481.1"
                     /translation="MNRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSMENVLKQGD
                     MVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKVVKEPYLT
                     KNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTINKTDILG
                     KARFVYYPLDEIKWIQ"
     gene            complement(629..1129)
                     /locus_tag="PWS82_002400"
     CDS             complement(629..1129)
                     /locus_tag="PWS82_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0907482.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1408..1806)
                     /locus_tag="PWS82_002401"
     CDS             complement(1408..1806)
                     /locus_tag="PWS82_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0907483.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2066..2926
                     /gene="ylqF"
                     /locus_tag="PWS82_002402"
     CDS             2066..2926
                     /gene="ylqF"
                     /locus_tag="PWS82_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL0907484.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2919..3686
                     /locus_tag="PWS82_002403"
     CDS             2919..3686
                     /locus_tag="PWS82_002403"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL0907485.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3742..4599
                     /gene="dprA"
                     /locus_tag="PWS82_002404"
     CDS             3742..4599
                     /gene="dprA"
                     /locus_tag="PWS82_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL0907486.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4721..6799
                     /gene="topA"
                     /locus_tag="PWS82_002405"
     CDS             4721..6799
                     /gene="topA"
                     /locus_tag="PWS82_002405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL0907487.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6768..8144
                     /gene="trmFO"
                     /locus_tag="PWS82_002406"
     CDS             6768..8144
                     /gene="trmFO"
                     /locus_tag="PWS82_002406"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL0907488.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2854 a         1444 c         1720 g         2170 t
ORIGIN      
        1 aaaaaacaac gctatattaa tcgcttctgg cttgtattca aatacttact tgtttctgta
       61 ttccttgcgt tcatgttgcg gggattttta ttcatcccgg taccagtgga aggcaattca
      121 atggaaaacg tgttgaagca aggtgatatg gttgtgatgg aaaaattttc ggagatcaga
      181 cgatttgata ttgttgtttt tcaattagca gatggcacga tttatatcaa acgagtaatc
      241 ggtcttccag gagaaaatgt cagctaccaa aatgatcaat tgaagatcaa tgggaaagta
      301 gttaaggaac cttatctaac taaaaacctg aaatcagatc atgcaaatgc atcctatacg
      361 acggacttta cactacaaga gctaacggga caatccaagc ttccggaaga taattatttt
      421 gtacttggag ataatcgaag agtttcaaaa gatagtcgtt cgtttggcac gatcaataaa
      481 actgatattc taggtaaagc aagatttgtc tattatccac ttgatgaaat aaaatggatc
      541 cagtgagaaa caataaagcg tgcagtctgt tgagactgca cgctttattg tttaatccaa
      601 aatcagttta ctaccttagt acgtaaaatc aaacagctcg tttaaacact tttctagcct
      661 ctaaagtttt gataaatgaa taaacaacca ttatttgaat agggatttgg atcatattcc
      721 tgatgaatct acctagaaaa attactttga aatctgcacc aaacaaaaca gacatcagcc
      781 aagaattgat aaaaaggctg actgggatca taatcaataa ttgggcagcg atgacggaag
      841 gcaggtttat ctttttatga tataaaaata agccattaat aaatccacca aggaaagccg
      901 tgataagaaa agcagcttga aacgttcctg aaccaaatat cgtgaaattt aaagtgttcg
      961 acaacatttc aagcaaacca gcccagatcg gtccatatag ataacccata agcgccatac
     1021 ttaaaaatcc gaatgtgatt tgaaaataag caggaccaat cgctatcttg tataagatga
     1081 catcaagggc taataaaaga gcagaggtcg acaacaatct tgcattcatt tcgggcaatc
     1141 taaaaacgtc ttttgcaatc aaagtgttca aaatattctc tcctttgtca gttaataata
     1201 aaaggatacc ataaaatcac caatgtgtca tgacacattg gtgatttaca tacactggca
     1261 tctaacaatt aaaaaaaggg tgagaagact gcttttataa actgtaaaaa agagattgta
     1321 cgaataaaaa aaatagaggc ggagaatact tatcgtatca tccgcctttg tttttttgtt
     1381 ataaaaaacg tcagattagt tatctgctta catcttcatc catacgggtt ctaatgtatt
     1441 tccgtctaga tcttggactt ctaatgaaaa catttcttct tcaggcattc ccatatctac
     1501 ttcgtggaaa gtaccaccat tttcttttgc aatttgagca aattttttta cttcatccgc
     1561 gttttcaaca ctaaacgaaa tcaaagcacc acttgttttt ttattatcag ctatttcttt
     1621 atcttttata aattttttat aaaaatcatg agagagaagc attatccaaa aagtgtcatc
     1681 ccataccatt gaaccagctt cttcagtaga aaattcttca ttttttttaa aacccagctt
     1741 ttcataaaaa gcaacggaac gattgacatc agcaacagga aaattaacaa aaaccatact
     1801 attcatttgt ttcgtctcct ttctgttttg ttcgtctatc atctcttatt taggaaacgc
     1861 tgtcaaaaat ttagattcat atatagaaac caaaataaaa atttgcatca tcaaaaaagt
     1921 gaagatgtga tagaatgagg actggttcac tttcacttaa ctaatagatg gaatttcttt
     1981 ataataaaaa agtgaattta tattgaaaaa gaaaaaaaca agtaaagccg gggaatattc
     2041 cggtatgaaa ggaatggaga gaacaatgac catccaatgg tttccgggac atatggcaaa
     2101 agcccgacga gaagtttctg aaaaaatgaa atatgtggac atcgtatttg aattggtaga
     2161 cgcacgatta cctttatctt ctcgtaatcc aatgctagat cagatcattc agcaaaaacc
     2221 tcgccttgtt ttattgaata aagcggatct ggcagatcca caacagacaa agctgtggca
     2281 agaatatttt cgtaaacaag gccatcttgc attagctatc acggcacaag aaagtaaagg
     2341 aattaaaggt ctagtacctg cggctaaaga agcattgaaa gaaaaacggg caagagatgc
     2401 tgctcggggg atcaagcctc gcgctattcg ggcaatggtc ttaggtattc caaatgttgg
     2461 gaaatctacg ttgatgaatc gactagtagg caagaaaatt gcacagacag gaaataagcc
     2521 tggcgtaaca aaaggtcagc agtggcttcg tttaggtaat gagttggaat tgttagacac
     2581 accagggatt ttatggccca aatttgaaga tgaagaaatc ggcaaaaaac tagcgctgac
     2641 aggcgcgatc aaagaccagc ttgtccattt agatgattta gcgatttacg gattagactt
     2701 ttttgcccga tattattcag gaagaatcag cgatcgttat ggtttatcaa tcgagcaaga
     2761 agaaaaattg gcaccggaat tactgatgga tattacagca aaacgtgggt ataaagaaga
     2821 ctatgatcgt ggcagcgaat tagtcgtgca tgatattcgt caaggaaaac ttggacgata
     2881 cacactggat cgttgcgaag aaatggagca aaaaagtgat gactgaatcc atccaaagta
     2941 tcaaagaacg attgaaaagc atacagagaa tagatgatcc tacactagaa tcattaagaa
     3001 atgatccacg caaaggcgtg cagcaagcac tggcttcttt tgaacgacga ttgaaaaagg
     3061 aagcattaat taaggaaaaa tatgaagaaa tgttttgctt tgaaaagcaa gggtacgcac
     3121 aaggtcacca atggattgcc ggcattgatg aagtaggaag aggaccatta gctggacctg
     3181 tcgtggcagc tgcagttatc ttgccgaaaa atgagatcat attagggctg aatgactcca
     3241 aacaattatc agagaaaaag cgagaacttc tttatcaaca agttcaagaa aaagccctgg
     3301 caatcgggat cgggatcgtt gatcagcaga caatcgatga tgtgaatatt tatgaagcaa
     3361 gtaaattagc gatgatccaa gcagtagaaa aattgtccat tgtaccaagc tatttgttga
     3421 tcgatgcgat gaccttggat ttgcctattc agcaggaaaa aataatcaaa ggggatgcac
     3481 gatctgtgtc gattgctgct gcaagtatca ttgctaaagt atatcgagac caattgatga
     3541 aagaatatga cgtgctttat cctggatatg gtttctcgca taatgcaggt tatggaacaa
     3601 aggaacactt agaaggactt gaacgacaag gaattacgcc aatccatcgt aaaagttttg
     3661 cacctgtaaa aaaatattta caataaaaaa cagccgcttt tacgtatctt attagaaaga
     3721 gagacgaaag gcggttttat gatgtatcaa atagaagaaa atttattgaa acttgcttat
     3781 acaaaaggaa tcagtagtca aggaaaatgg gcacttgcta actggatgat gcagtatcct
     3841 tataaagaaa tcggatttga agacatcatt aagataggta aaataaccaa acatcgggag
     3901 ctgtttcgca aaagttggca ggaaattcac gctaactggg gcaaaatcca atcgaaacag
     3961 tcgtttatca cttgctttga tcccaaatat ccccctcagc tgcttcactt gacctatccg
     4021 ccaattgtac tgttttataa tggtgatcta tctttacttt cttgtaacat gttaagcgtt
     4081 gttggtggaa gacacacttc tgctttagcc aaaaaaacag tttattattt actaaaacca
     4141 gtgatagaag ctggatatgt gatcgtcagt ggctgcgcga aagggataga tacatacggt
     4201 caccaagcag caatctctca tggtggacgg acaatcgcag tcattggaac agggatagag
     4261 caagtctatc ctgctgaaaa tcgtgacttg caagaggtga ttgcaaatga tcatcttctt
     4321 ttgagtgaat atcctcctca atccggcccg aaaaagcatt gttttccaat gagaaatcgg
     4381 attatcgcag ctttgtctaa aggaacatgt gtctttgaag ccaagaaaaa aagcggctct
     4441 ctgataacag cacagcaagc attggatctt ggaagaacag ttttttctgt tccaggaaat
     4501 attttaacag aacattctac cggatgtcat catttgatcc aagacggtgc agtttgcacg
     4561 gtttcgggtg aagatatctt ggcagaattt aaagaataac accatacttt tcatttgctt
     4621 ttcttgacaa acgttttctg aatggatatg attagctacg atttgtatga atcctctttt
     4681 aatagtagga acacgacaat ggaaaggagt ccattcagta atggcgtata aatatttagt
     4741 gattgtagaa tcacctgcaa aagccaagac aattgagaaa tatcttggac gaaactataa
     4801 agtaatggcc agtgttggcc atataagaga tttaccgaaa agtaaaatgg gtatcgattt
     4861 tgaaaacaat tatgaacccc attatatttc tatacgcgga aaaggcgatg tcatcaaaag
     4921 cctgaaagcc gcagcaaaaa aagctcaaaa agtttacttg gcaagtgacc cggatagaga
     4981 aggagaagcg attgcttggc atttagcgta cctacttggg ttggatctga aagaaaaaaa
     5041 tcgggtggtc ttcaatgaaa tcacaaaaga cgcagtcaaa gcagctttta aggaaccaag
     5101 aacgatcgat gtagatttag tagatgcaca gcaagctcgt cgtaccttag acagaatcgt
     5161 tggttattcg atcagtccta ttctctggcg taaggtcaag aaagggttaa gtgcaggacg
     5221 tgtccaatct gtcgctttaa aaattattat tgaccgtgaa aaagagatcc gagaatttgt
     5281 tccagaagaa tattggagca tcgacggtaa ttttaaaaaa gctcgcaaga aattcaaagc
     5341 aaatttctgg ggaatcgacg gtaagaaaaa gaaattacca gatgcacaaa gtgtaaaaga
     5401 agtcactgct agaattgatg gaacagactt cgaagttaaa aaagtagaaa agaaagagcg
     5461 caaaagaaac cctgcgaatc ctttcactac aagtagcctg caacaagaag ctgcccgtaa
     5521 gttaaacttt cgaacaagaa aaaccatgat ggtagcacag caattatatg aaggaatcac
     5581 tttgggtaaa caaggaacag tcggcttgat tacatacatg cgtactgatt ctacccggat
     5641 tgccgataca gcaaaagcag aggccgcagc atttattgaa gatacgtatg gaaaagaatt
     5701 ttctagacat gatcaccgaa agatcaaaaa tgctcaaggc gcacaagatg cccatgaagc
     5761 tgtgcgtcca acgagtgtgc ttcgtacacc agatgaactc aaacaatatt tagataaaga
     5821 tcaattgaaa ctttatacat tgatctggtc acgttttgtg gctagtcaga tgacaccagc
     5881 tattttagat acaatgaaag tgacattaga acaaaatggt gtcatcttca ttgcgaacgg
     5941 ttcaaaaatc aaattcaaag gattcatgca agtgtatgtg gaaggtagag atgacggcaa
     6001 agaagagaaa gaaaatattc ttccagaact tgaagaaggc gatgtagtcc aatctgttga
     6061 tattgaacca aaacaacatt ttactcaacc accagcacgt tttagtgagg ctacgttgat
     6121 tcgtgcacta gaagaaaacg gggtagggcg accttccact tatgcaccaa cacttgatac
     6181 gatccaaaga cgctactatg taaaattgac acaaaaaaga tttgagccga ctgaactagg
     6241 agaaattgtc aattcattaa tctgtgaatt cttccctcaa atcgtcgata tccatttcac
     6301 agcagaaatg gaaggagatt tggataaaat tgaagaaggc acggaagcgt gggtcaaagt
     6361 ggttgatcgt ttctataaac cgtttgaaaa agaactgacg aatgcggaag aaaaaatcga
     6421 aaaaattcaa atcaaagatg aaccagcagg attcgattgt gatgtctgtg gacatccgat
     6481 ggtgataaaa ttagggaaat atgggaagtt ttatgcttgc agtaattttc ctgattgtcg
     6541 gaatacgaaa ccaatcgtaa aagaaatcgg tgtgacttgc ccagtatgtc atgaaggaca
     6601 agtcattgaa cgaaaatcta agaaaaacag actattctat ggttgttccc gtttcccaga
     6661 atgtgaattc acttcttggg ataaaccagt gggcagaaat tgtccaaaat gtgacggata
     6721 tctggtcgag aagaaagtca aaggcggtaa acaagtagtc tgtgtaaatg gagattacga
     6781 agaagacatc caaaaataat agaaaagagg ttctttcatt gaaaatggta aatgttgttg
     6841 gagcagggct tgccggaagt gaagctgctt ggcagatcgc acaagcagga gtccctgtcc
     6901 gtttatatga aatgcgtcca gtcaaatcga ctgaggcgca tcacacaagt aattttgctg
     6961 aactagtatg ttccaattca ttaagaggaa acagtttggc taatgcagta gggttattga
     7021 aagaagaaat gcgccgattg aattcggtag taatcacttc ggcagatgaa acagcagtgc
     7081 cagctggtgg tgccttagct gttgaccgcg attctttttc tgaaacaatt acccgaaaag
     7141 tcaaagagca tccattagta acagtgataa acgaagaaat tactgaaata ccggaaggaa
     7201 tcaccgtagt agcaacagga cctttgactt ctcatccatt agcagaaagc atcaaagcat
     7261 tcaatggttc agatggtttt tatttctatg atgcagctgc accaatcgta gacaaagcta
     7321 cgatcgatat ggataaagta tatttgaagt cccgctatga caaaggagaa gcagcttact
     7381 tgaactgtcc gatgacagaa gaggaattca aagctttcca tgaggcacta atcaatgccg
     7441 aagtggcaga acttaaatct tttgagaaag aaaaatattt tgaaggatgt atgccaattg
     7501 aagtaatggc acagcgagga ttcaaaacga tgcttttcgg acctatgaaa ccagttggat
     7561 tggaagatcc taaaactggc aaacgtcctt atgctgttat ccagctgcgt caagacaatg
     7621 ctgcagcttc tttatacaac atcgtgggct ttcagactca cttgaaatgg ggcgagcaaa
     7681 aacgtgtttt ccggatgatt cctgggttag aaaatgctga attcgttcgc tatggtgtga
     7741 tgcaccgaaa cagctttatg aattcgccag aattgctgaa accgacttac caatcaacga
     7801 aacgcgaaga tctgttcttt gcaggtcaga tgactggtgt agaaggctat gttgaaagtg
     7861 cagcgagcgg actacttgct ggaatcaacg ccgcacgctt agcaaaagaa atggaaccag
     7921 tcgagtttcc acaagaaaca gctattggaa gtatggctta ctacattact catgcggaag
     7981 gaaaacattt ccaaccgatg aatgccaact atggtctgtt tcctgaactg ccagagcgca
     8041 ttcgagacaa aaaagcaaga tatgaagcaa ttgcacaacg agctttaaaa gctaatgagg
     8101 caataaaaac agaattagaa aaaactgcaa tcccaaatga gtaaggaaac tccatgaaga
     8161 gggtgagacg gatagtctcg ctcttttt
//
LOCUS       DANJJW010000114         8066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000114 DANJJW010000000
VERSION     DANJJW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(6..320)
                     /locus_tag="PWS82_002407"
     CDS             complement(6..320)
                     /locus_tag="PWS82_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907489.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            606..845
                     /locus_tag="PWS82_002408"
     CDS             606..845
                     /locus_tag="PWS82_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907490.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1207..1467)
                     /locus_tag="PWS82_002409"
     CDS             complement(1207..1467)
                     /locus_tag="PWS82_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907491.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1652..2149)
                     /locus_tag="PWS82_002410"
     CDS             complement(1652..2149)
                     /locus_tag="PWS82_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL0907492.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2279..2989)
                     /gene="budA"
                     /locus_tag="PWS82_002411"
     CDS             complement(2279..2989)
                     /gene="budA"
                     /locus_tag="PWS82_002411"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL0907493.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3002..4666)
                     /gene="alsS"
                     /locus_tag="PWS82_002412"
     CDS             complement(3002..4666)
                     /gene="alsS"
                     /locus_tag="PWS82_002412"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL0907494.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4874..5536)
                     /locus_tag="PWS82_002413"
     CDS             complement(4874..5536)
                     /locus_tag="PWS82_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0907495.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5546..6361)
                     /locus_tag="PWS82_002414"
     CDS             complement(5546..6361)
                     /locus_tag="PWS82_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0907496.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6622..7065)
                     /locus_tag="PWS82_002415"
     CDS             complement(6622..7065)
                     /locus_tag="PWS82_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL0907497.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7199..7537)
                     /locus_tag="PWS82_002416"
                     /pseudo
     CDS             complement(7199..7537)
                     /locus_tag="PWS82_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7525..7902)
                     /locus_tag="PWS82_002417"
     CDS             complement(7525..7902)
                     /locus_tag="PWS82_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0907498.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
BASE COUNT         2508 a         1645 c         1228 g         2685 t
ORIGIN      
        1 ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca
       61 gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa
      121 gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt
      181 gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca
      241 tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata
      301 aaattactgt aaaaatccaa aagctatcct cctagcaact atttaaatac ccaaaaaaat
      361 tacctctaga taatatcata cctacgtttt cacttataaa ttttttcata aaaaaagcgt
      421 ttagaggcat aatcgttaat acagatggta accaatgtat ctggtttgaa ctgggatatc
      481 accctctcca tatatgtatt actaagacta atagatacta taaaagactt gatagttttg
      541 cattgatttt gcctaatact gagcataggt gtatgctaaa cgatactata aggagtgtgt
      601 acataatgaa aagaattctg tttatcagtt tatcaaattt attgttactt actgcttgtg
      661 ggacttctgc atcaaagaac ataccttcag atacgcaaat ttcgaatagt atcgattcaa
      721 caatttctac aaatgagaaa aattctccgg tagaaacttc cgatataaaa gtttctacct
      781 ctgcaaaaaa cagggtaaag aaactgtcag cacgtctcct ttatttcaaa caaactctga
      841 attagcttct tcatcttctt cagcaaataa tgatgatagt ttcgcgttta ctcctaacta
      901 aatgcaacct ttacaaatgc tttacgataa caagcctgag ctaaatgatc ctgatatcag
      961 ttttgcattt tttgaaatga ttggatcgga tgatcttttt aaaactacta gtttgtctat
     1021 ccgaaaatag gacagttctg gcagtatagg tttttatcga gtttctccac caggcctcgt
     1081 tacagagaca gattctctag gcgaaccttt tgaataaatc aaaaatcatg aacaatcact
     1141 gccaactaaa taaagtgcct tcaaaagctt ggtcttctcg cttttgaagg cactttattt
     1201 aaatctttat gaaactaaag tatgttcata tttgttgcta tagttatata gatattctgt
     1261 tgtttccaac tcataaacgt gagtatattt tgccactact tttccgcatt tttctaatat
     1321 ttcattgcga tcgacgtcat gatattcgtc tacgcagatc acaacacaat tttctaattt
     1381 actgattact tctccaacca ctttttgttt caagccaata ggttggcaaa tatatttttt
     1441 tcctaaaatg atagtttcgt tattcatatt tcatccatcc ttaatgtttt ttcattattc
     1501 aaaatgataa taccattaaa atatgattgt gaaaatcaat aacacttgtg attttataaa
     1561 aaaataaaaa aaccgaaaaa cagaaatgac cactgtcttt ccggctcttt ttattcttga
     1621 tttctcttct tttttgtttc aatagacgat tttagttatt aataaatgaa aaagcattta
     1681 tattaaaatt tgctaataaa agatctacat atttttctag aaatgcttga tcgatttcat
     1741 catacccttt cgttactccg caatcgatat ctaaaacacc gattaaatta ccttctttta
     1801 ccattggcac tacgatttct gattgtgcgg ctgaatcgca ggaaatgtaa tttgcatggg
     1861 ttttgacatc atcaacgatc aatgtttctt gtttttcagc tgattctcca caaactcctt
     1921 ttcccatctt gatacgtgtg caagaaactc gcccttggaa cggtcctaaa atcaattcat
     1981 taccatcgaa taaataatat cctgcaaaga cagtctcagg caaagtctca ttcaacaatg
     2041 cagatgaatt tgctaaattt gctaaccagt tatcttctgc ttctaataat ccttgttgct
     2101 gtaataataa aatttcgtat gcatcttttt tctgtttttc tgtccacatg ataaaaaccg
     2161 ctcctttttg atctatggag aagattatag ctcagttttt ttgtaaacgc taggtttcct
     2221 cctcaaaaaa acgagatacg tcaggttaat caccttgaac gcatctcgct gtctttttct
     2281 attctgcttg ttggatttct gctgctaaat cagcataatc aatcgtttcg tgaacaaaag
     2341 tcggatcatc aactggaaaa tgctgtacaa gggacgagat gcggctaatc tcaacttttc
     2401 cttcagccac ttcaaaatcc attacatgtc cgccaaatgt atgtgtatca tcgataaaat
     2461 gcaggtgaaa tcccgctgca gcaactcctt ggaataattc aggcgtaaag aacccgacta
     2521 atgttccttg gatattgctt tgttggaatt ctggttgaga ctctgaaatt tttgctagtc
     2581 ttctgtaagg tttttcttgc ttcggcatga tacggatgtg catatttttg aataatccag
     2641 ttatcttcac tgccaaaaat aggttttccc cggcttctct tactaatatg tcttttttca
     2701 attgttctcc atctgcagtt tttgagacag aaaaactagt atctggcgta aaatctgtaa
     2761 ttgctgcata gggggtaagt tcacttccat ctaattgggc aactgagcca tctgatcttc
     2821 cttgataagc tacgccatct aaaaagatga cttcaccatc aagcccatgc aatgttccca
     2881 atcccagagt tcctttttct aatatatgag caatcgtttc tgttccatcc atcaagccgg
     2941 ccatcaaagc tcctaatgtc ccgtgctggt ataagatttt ttcagtcatg tcgacacttc
     3001 cttaatagaa ttgatctggt aagattgttt ctcccaattt ctcattatca cggtaatcaa
     3061 tcggaatatc aataattacg ggtccttctg tcgcaaatcc ttcttctaaa gctttttcta
     3121 attcctctac acttgtagct cggattcctt tagcgccaaa agcttctgca tatttcacaa
     3181 aatcaactgg accaaagtct acacctgacg cccgctgata tttcattttt tcttggaact
     3241 ctaccatatt gtagtgtcca tcattccaaa tcaaatgaac gatattcaat tttttacgta
     3301 ccgccgtttc taaatcctgg gcagagaata aaaatccccc atcgccggaa actgatacga
     3361 tttgtgtatt tggacgcacc aatgcagcag aaatcgccca tggcaatgca acaccaagcg
     3421 tttgcatacc attactgaat aacaaatgac gaggttcata acttctaaag tggcgtgcca
     3481 tccaaatata atgactacct acatctacgg taacagtcat atcatctgtt acttttgact
     3541 gaagcgtgtt aataacttct aaaggatgca agatacctgc ttctcctttt gattgaacaa
     3601 tgtcccgctc cgttaatttt gcttgaagag atgctaaata ttcttttgca tcttctgaca
     3661 cttgttgagg ctctaatgaa cctgtcaata aatcaagagt ggctgagata tcaccgatca
     3721 attctctttc aggctgcata aaaggatcga tttcagctgg cgcttcatcg atcacaatga
     3781 tccgcgcatc tttttccgca ttccagtttc ttgcttcata ttcgattgga tcatagccaa
     3841 ttgcaataac tagatcactt cgtttcaaaa gcatatctcc tggttggttt ctaaataaac
     3901 ccacacgacc aaagaaatgc gcttctaatt cacgtgagat cactccagct gcttggaatg
     3961 tctctacaac aggcaatgct gtcttttcaa ctaattgcct gatggctaac gtttcttttt
     4021 cacttgatcc tcgcatacct actaatagta ccggtagttt tgcttctcta atgcgttcag
     4081 ctaggtaacg gatatcatct gcagaagcag aacctaattt aggatcgctc aatggcttga
     4141 tgacattacc ttggacaggt gcatccacta catcttgcgg gatactgata aagctcgcgc
     4201 ctttttttga actcttagcc atacgataag cattggcaat cacttctgaa attgtttctg
     4261 gatcttggat ttctgcactg tattttgtga ttggctggaa cagtgctgca ttatccatgc
     4321 tttgatgggt caacttcaat agatcgcttc gttttacttg tccggcaatt gctaaaactg
     4381 ggtcaccttc tgctgtcgct gttaccaaac ctgtagctaa gttactagca ccaggaccac
     4441 ttgttgcgat aactactcct ggctctcctg taatacgtcc gattgcttgt gccataaatg
     4501 cagcattttg ttcgtgacga gtcacgatta attcaggacc ttgatcctct aattcattaa
     4561 atacaccatc gatttttgca cctggtatac cgaatacata atcaacatga tgattaatca
     4621 aactttctac tacagatttc gaaccttgtt tcattttttc agccattgaa catcgtcctt
     4681 tttatgcttt atttttaatt attagttaga aataatcaca aataagtgat acagtttcta
     4741 aacctgtttc atacaaaagt atagcacgtt ttatttcatt tgtttagcca tatgtttcaa
     4801 aaaatgaaaa agaatgacag tttttacata aaaaaaactg tcattcttaa ttgaaagttt
     4861 caatttataa agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca
     4921 aaccaaatac gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga
     4981 gaaacaaact tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat
     5041 agtagaacgc tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat
     5101 accagcagtt ggtgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca
     5161 accattttcc ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa
     5221 ctatccaagc aaatagccaa taaaaaaatc cctttagcca aaattggcga aacatcttta
     5281 tgaacaaacg tactggtgaa aaactatttt cttttgctaa ttcttctgtc atacctatca
     5341 tcacgatgca acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc
     5401 catctatcga caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa
     5461 ggctacttgc caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa
     5521 aacgctgctc tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta
     5581 caaaatattt ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg
     5641 cagccatgat taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact
     5701 ggattgtttt attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg
     5761 tattaacgaa tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga
     5821 tcttgcgata gattccccac tctgataagc catcgatccg cgcactctca cataaagaat
     5881 ctgggatact actgtaatat tgcttcaaca agaacacacc aaaagcattg aacgcttgta
     5941 atagcaccaa cgaccacaac gtatctgtta aacccacttt tctcatcata atgaactgcg
     6001 ggatcatata agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat
     6061 cgcgcccttt aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact
     6121 gaatcaacgt gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa
     6181 tacgagacca aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg
     6241 gaatcgtgaa cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa
     6301 tgatcgtcaa taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca
     6361 tgttttttga acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat
     6421 cttcatttct ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc
     6481 gttcttctta tttttgtaag cgcttattta acaagttatg taaatctctt tatttttttg
     6541 atttaaaaat gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt gtattgaaat
     6601 ttcttcgatt agagatactt attactgttc aaaagcttct ttgaaacggt gaatcaattc
     6661 attgactaca ggtgataaaa tccgattctt tttccagact agcatgcagt tcgtttcaat
     6721 cattgggtca agcggaacga atgtcactcg ctttgaatga tgacttacag caccttctat
     6781 gactaaagca gcacctacct gattctcaat tagcgcaaat acattgaaaa tcaaattgta
     6841 cgttcctgtg atcagcaact gctccttttt tatgcctaac cagtcagcaa acattcgctg
     6901 tatttctgga cgctttgaga tcaaaagagg tacattcgca aagtcttctt ttgttatcac
     6961 gtcttttttt gctaaaacag aattttctaa aatgagcaat ccccattttt cctttcttgg
     7021 taacaaaatc gtttcgtatt tctctgtatc tactggatct agcaaaatcg ctaaatctaa
     7081 caggcttttt tctagtcact ccgtgatatc atcacttgtt ccgctgtgga tatgaaattg
     7141 aacgctggga taatcacgga tcaacttttc tagtaatagt gccaatgtat tcgaactatc
     7201 agcttccaca cagccgatcg taaatcggcc gctgaataat tgtttttttc tgttctcgaa
     7261 ttcttgagtg gtttgattcg ttaaatctaa aatttcttct gctcggcttt tttagaaaat
     7321 aacctgcttc tgtcaagatc attttgcggt tttcgcgtac aaacagttct gttcctagtt
     7381 actcttctaa ctccttcagt tgtcggctta aggttggttg tgtaagatgt agaacttctg
     7441 cggcttgaga gattgttcct tcttctgcaa ttgtccaaaa atattttagt aagcgtaact
     7501 ccatagcgat gctccttgat ttttttactt gatacattcc attttatttc tagataacac
     7561 acgatcatat ttcttgattt ttccttgtaa acgttccaaa cattcttgca tttcagcaat
     7621 ttttttcgtc aaaagttcac tttcttcaat caaaagctta cgccgctcaa atacagttgc
     7681 ttctccttcg ctagataggc gaatatattc aatcaaagac tcgatcgata atccggcatt
     7741 tcgcatacag atcgtaaagt cgacccattt taggtcttct tctgtataat cacggattcc
     7801 gccttttgtc cgattaactg gtggaatcaa cccgattcgt tcataatagc gcaatgtatc
     7861 ggccgaaatg cccttttctt cactgacttg tttgatattc atctgatcac tctttctctt
     7921 aatttcttgc cactttactt atgtatttta tttattctac aactttaagt gtactccaag
     7981 tcaatactca tttctattaa aactaagctg gattatctat atcggttgtt tgtgtattat
     8041 ttcaaataaa aaagtcttgt tttttt
//
LOCUS       DANJJW010000115         7926 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000115 DANJJW010000000
VERSION     DANJJW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..199)
                     /locus_tag="PWS82_002418"
     CDS             complement(<1..199)
                     /locus_tag="PWS82_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907499.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="PWS82_002419"
     CDS             complement(362..1996)
                     /locus_tag="PWS82_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907500.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="PWS82_002420"
     CDS             complement(2000..3373)
                     /locus_tag="PWS82_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907501.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="PWS82_002421"
     CDS             complement(3389..3628)
                     /locus_tag="PWS82_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907502.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="PWS82_002422"
     CDS             complement(3810..6485)
                     /locus_tag="PWS82_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL0907503.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="PWS82_002423"
     CDS             complement(6668..7591)
                     /locus_tag="PWS82_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907504.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1560 c         1198 g         2840 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatatt
//
LOCUS       DANJJW010000116         7908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000116 DANJJW010000000
VERSION     DANJJW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(19..993)
                     /locus_tag="PWS82_002424"
     CDS             complement(19..993)
                     /locus_tag="PWS82_002424"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0907505.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1073..1948)
                     /locus_tag="PWS82_002425"
     CDS             complement(1073..1948)
                     /locus_tag="PWS82_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL0907506.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2078..3985
                     /locus_tag="PWS82_002426"
     CDS             2078..3985
                     /locus_tag="PWS82_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL0907507.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4526..7804)
                     /locus_tag="PWS82_002427"
     CDS             complement(4526..7804)
                     /locus_tag="PWS82_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL0907508.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2240 a         1664 c         1465 g         2539 t
ORIGIN      
        1 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
       61 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
      121 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
      181 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
      241 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
      301 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
      361 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
      421 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
      481 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
      541 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
      601 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
      661 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
      721 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
      781 gtcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
      841 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
      901 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
      961 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
     1021 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
     1081 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
     1141 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
     1201 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
     1261 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
     1321 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
     1381 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
     1441 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
     1501 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
     1561 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
     1621 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
     1681 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
     1741 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
     1801 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
     1861 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
     1921 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
     1981 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
     2041 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
     2101 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
     2161 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
     2221 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
     2281 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
     2341 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
     2401 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
     2461 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
     2521 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
     2581 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
     2641 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
     2701 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
     2761 actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata aagtgatcgt
     2821 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
     2881 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
     2941 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
     3001 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
     3061 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
     3121 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
     3181 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
     3241 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
     3301 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
     3361 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
     3421 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
     3481 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
     3541 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
     3601 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
     3661 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
     3721 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
     3781 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
     3841 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
     3901 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
     3961 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
     4021 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
     4081 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
     4141 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
     4201 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
     4261 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
     4321 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
     4381 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
     4441 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
     4501 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
     4561 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
     4621 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
     4681 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
     4741 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
     4801 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
     4861 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
     4921 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
     4981 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
     5041 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
     5101 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
     5161 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
     5221 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
     5281 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
     5341 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
     5401 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
     5461 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
     5521 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
     5581 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
     5641 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
     5701 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
     5761 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
     5821 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
     5881 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
     5941 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
     6001 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
     6061 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
     6121 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
     6181 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
     6241 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
     6301 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
     6361 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
     6421 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
     6481 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
     6541 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
     6601 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
     6661 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
     6721 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
     6781 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
     6841 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
     6901 gatcgtaata atatgactct aaaccagtat tcgttttttt catctctacg tccagtttag
     6961 taatcgagtt cgtggccgga aacaaattcc catgaccgtt tccgaatata ttataagaac
     7021 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
     7081 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
     7141 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
     7201 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
     7261 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
     7321 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
     7381 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
     7441 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
     7501 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
     7561 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
     7621 tatcttcact agttcttggt acttcctcca ttgttgacat cccttcaatg ttcacgtgtg
     7681 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
     7741 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttta
     7801 acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt acctaattta
     7861 tttttactgt caataaaaaa caaaattata ataagtgtat tttataaa
//
LOCUS       DANJJW010000117         7903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000117 DANJJW010000000
VERSION     DANJJW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            234..2000
                     /locus_tag="PWS82_002428"
     CDS             234..2000
                     /locus_tag="PWS82_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL0907509.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2102..3361
                     /locus_tag="PWS82_002429"
     CDS             2102..3361
                     /locus_tag="PWS82_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0907510.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3508..4809
                     /locus_tag="PWS82_002430"
     CDS             3508..4809
                     /locus_tag="PWS82_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0907511.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4812..5663
                     /locus_tag="PWS82_002431"
     CDS             4812..5663
                     /locus_tag="PWS82_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0907512.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5676..6491
                     /locus_tag="PWS82_002432"
     CDS             5676..6491
                     /locus_tag="PWS82_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL0907513.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6642..7808
                     /locus_tag="PWS82_002433"
     CDS             6642..7808
                     /locus_tag="PWS82_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0907514.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2578 a         1365 c         1583 g         2377 t
ORIGIN      
        1 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
       61 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
      121 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
      181 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
      241 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
      301 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
      361 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
      421 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
      481 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
      541 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
      601 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
      661 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
      721 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
      781 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
      841 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
      901 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
      961 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
     1021 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
     1081 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
     1141 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
     1201 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
     1261 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
     1321 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
     1381 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
     1441 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
     1501 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
     1561 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
     1621 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
     1681 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
     1741 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
     1801 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
     1861 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
     1921 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
     1981 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
     2041 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
     2101 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
     2161 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
     2221 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
     2281 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
     2341 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
     2401 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
     2461 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
     2521 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
     2581 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
     2641 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
     2701 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
     2761 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
     2821 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
     2881 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
     2941 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
     3001 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
     3061 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
     3121 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
     3181 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
     3241 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
     3301 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
     3361 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
     3421 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
     3481 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
     3541 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
     3601 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
     3661 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
     3721 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
     3781 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
     3841 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
     3901 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
     3961 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
     4021 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
     4081 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
     4141 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
     4201 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
     4261 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
     4321 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
     4381 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
     4441 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
     4501 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
     4561 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
     4621 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
     4681 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
     4741 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
     4801 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
     4861 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
     4921 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
     4981 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
     5041 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
     5101 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
     5161 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
     5221 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
     5281 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
     5341 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
     5401 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
     5461 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
     5521 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
     5581 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
     5641 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
     5701 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
     5761 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
     5821 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
     5881 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
     5941 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
     6001 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
     6061 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
     6121 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
     6181 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
     6241 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
     6301 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
     6361 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
     6421 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
     6481 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
     6541 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
     6601 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
     6661 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
     6721 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
     6781 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
     6841 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
     6901 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
     6961 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
     7021 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
     7081 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
     7141 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
     7201 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
     7261 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
     7321 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
     7381 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
     7441 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
     7501 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
     7561 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
     7621 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
     7681 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
     7741 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
     7801 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
     7861 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtc
//
LOCUS       DANJJW010000118         7721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000118 DANJJW010000000
VERSION     DANJJW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            291..764
                     /locus_tag="PWS82_002434"
     CDS             291..764
                     /locus_tag="PWS82_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL0907515.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            761..2893
                     /gene="feoB"
                     /locus_tag="PWS82_002435"
     CDS             761..2893
                     /gene="feoB"
                     /locus_tag="PWS82_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL0907516.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2895..3029
                     /locus_tag="PWS82_002436"
     CDS             2895..3029
                     /locus_tag="PWS82_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL0907517.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3123..3767
                     /locus_tag="PWS82_002437"
     CDS             3123..3767
                     /locus_tag="PWS82_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907518.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3954..5147
                     /locus_tag="PWS82_002438"
     CDS             3954..5147
                     /locus_tag="PWS82_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0907519.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5140..6867
                     /locus_tag="PWS82_002439"
     CDS             5140..6867
                     /locus_tag="PWS82_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL0907520.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7261..7458
                     /locus_tag="PWS82_002440"
     CDS             7261..7458
                     /locus_tag="PWS82_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL0907521.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7460..>7721
                     /locus_tag="PWS82_002441"
     CDS             7460..>7721
                     /locus_tag="PWS82_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0907522.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
BASE COUNT         2542 a         1300 c         1690 g         2189 t
ORIGIN      
        1 tactaagttt tgtgtttttg aaaaaagagc tgaaaacaag tatcgtgtgg gaaaatagca
       61 aatttaatag agaaaaaatt gtggaaaact atgaagaaat atttttttct catttttatc
      121 tatcaatact aaagattcgc tcataatatc acaagtataa ttgacattct caattaccga
      181 gagtattcta tgtgagaatg attctcattt atttgcgtaa cacgtaagaa aattagaaca
      241 tataatgaat gattttaata catgatttat ttcacaagag aggaatcaat atgcagacct
      301 tagcgcaggc aaagcagaat ttcgtttatc aagtcgaacg aatcgaaaca gaagaaaaaa
      361 caaaacgcca tttgcaaaat atcgggatcg ttgccggaag taaaatcgtc gtcgtcaatt
      421 tatcaggcaa caatgccatt ttactggtaa aaagtcatcg agtggcagta agtcgggaaa
      481 tactaggtca aatctttgtc aataaaaaga ctgcttcctc tgaaagctgg acttctcttg
      541 atcaattaaa agcaggagag actgggatcg tcgtagcaat ccacggacaa ggcgcagtga
      601 agcgacggtt aatggatatg ggattgacgc gaaatgtaaa aatagttatc cgaaaactag
      661 cccctctggg agatccaatc gaactgacag tgcgagggta tgaactgacg ttacgaaagt
      721 cagaggccga actaatcttg atacaaaggg aggaaaaaga atgaatatcg cgttagtcgg
      781 aaatccaaac agcgggaaaa ctagcttgtt caatctcttg actggttcga atcaatatgt
      841 agggaattgg ccgggcgtaa cagtagaaaa aaaagaagga tcgttgaaaa aaaatccgaa
      901 cattcatatt caggacctac ccggcatcta ttctttgtct ccttatacac cagaagaagt
      961 gattgcaaga gattatttgg ttcaggaaca gcctgaactg atcatcaaca ttattgatgc
     1021 taccaattta gaacgaaatc tttatttaac tacgcagtta ttggatttag acattcctat
     1081 gatcattggt ctgaatatga tggatgtcat caaaaaagaa ggaaaaaaaa tcaatctaga
     1141 aaagctttcc tatggtcttg gtgtaccagt tttaccaatc agcgtattga aaaagcaagg
     1201 aatcgaaaaa ctaatcgaaa agattcagga gtctgcaaaa aagcggcctt ctattcctgt
     1261 tccctgtaca tatgatccac gtttagaagc agctttacat gaaattgaag atgtactagg
     1321 agaacaaagc ggcaagaaac gctggtatgc aatgaaatat tttgaacgag atcagaaggt
     1381 aagagaggat tacgagatag cagtttctca gcaaaaagag atcgagcaac tgatccagtt
     1441 aacagagaaa ctattggacg acgatagtga gacgattctg gtcaatgaac gttatgaatt
     1501 tattacccaa ctatgtacac tctgtgttgt gtcaaatgat tctttccaat tatcgatgag
     1561 tgacaaaatc gatcagattg caacgaatcg ttggttagcc ttgccgatct tcgcatttgt
     1621 gatgtggctg atctattatc tggctattca aacagtagga acaatgggga cagattggat
     1681 caatgacaca ttgtttggta catggttacc agaacatgtt ggcagattgt tagctgaatg
     1741 gcaagtagcg ggatggatgc aggagctgat cttgaacggc atcatcgcag gcgtgggagc
     1801 agtgttagga tttctaccgc aattgattat attgttttta tgcttgagct ttctggaaga
     1861 ctgcggatat atggcgagga tcgcttttgt catggatcga cttttcagga aattcggtct
     1921 ttcaggaaaa tccttcattc ctatgctgat tgctacagga tgtggagtgc caggcgtcat
     1981 ggccagtcgc acgattgaaa acgagcaaga tcgtcgtttg acgatcatgg tcacaacctt
     2041 catgccttgt tctgcaaagt tgccgattat tgccttagta gcaggggcct tttttcctca
     2101 tcaatcttgg gtagctcctt ctgcctattt tcttggtatg actgcaatta ttttctctgg
     2161 aatcagcttg aaaaaaacac gtttgtttgc tggagataca gctcctttta tcatggaatt
     2221 gcccgcatat cattttcctc aatggtcagc ggtcctaaga caaacctatg accgcagcaa
     2281 gtcttttgtt aaaaaagcag gaacgattat ctttgtttct agtatcgtga tctggttcag
     2341 ctcttcttat aatttttatg ctcaacctgt ttctgaagac caaagtatct tagccttttt
     2401 cgggcgaatc ctggcagttg tttttcagcc gcttggttgg ggaaactggc aaggaacagt
     2461 cgcagcgatt acaggattgg tcgctaaaga aaatattatt ggcacatttg gtattttgtt
     2521 tggtcatgcg gaagtatccg aaaatggcag agaaatctgg caagtgctac aacacacata
     2581 tactcccgca gcagcttatt cattgttagc cttcaattta ctttgtgcgc cttgttttgc
     2641 ggcaattggg gctatccatc gggaaatgaa tgccaagaaa tggacttgga tcgccatagg
     2701 ctatcaatgc ggtttagctt atttagttag ttttgtgatt tatcagttgg gacacttatt
     2761 ggagggtgga caggtagcgt tagggacgta tcttgctatt ttactgatga taggactcgt
     2821 ttatgttttg ttccgtcagc caaaatccaa gaaccagttt tatactatat tgtcattaga
     2881 aggagaggaa taggatggcg acattcttat taagtgtgtt aatctttgga agtgcaggcg
     2941 caattgtcta tactcgttta aaatccggta agaattgtga tgactgtcaa acagcttgcc
     3001 cagtaaaaaa ggaacagatc aagcgatgat cagtcgttgc aagaggctat tctccccttt
     3061 tttgcttatt ttgctagagc atagtaagat gagggaaagt aaaaaaagaa agaggaaaga
     3121 aaatgtctgg aaagaaaaaa atcgtccgcc cgcgctacca gcagattgct gttgatttag
     3181 ccgaacgtat cgttgaaaac cgatataaag tgggtgagaa gatccatgcc cgctcaacac
     3241 ttgccagtac gtacaatgct tctcctgaga ctacccgcaa ggctatcaat gtgctggtag
     3301 atcttggaat catggaagta cggcatggaa gtggggcatt tgttgcttcg aaggaaaaag
     3361 caaaagattt cctattgcaa tatcaagatg tacaatccat ccaagataca aaagcagaaa
     3421 tcatggcaag tgtggagaag caacaagatg agctgaatca tttttctcaa ttattggatc
     3481 agctggtcaa tcaaacgaag cgaatacata agatgaatcc tttacttccg tttgaattat
     3541 ctttatcaga aaaagcaatg catctggacc gttcaattag cgagttgaat atctggcagg
     3601 cgactggcgc cacagttatc gcaatttctc ataatgaaga actgcttgtt tcacctggcc
     3661 cctatgctaa atttacggca ggggacatcg ttcattttat tggaaatgaa tatacactac
     3721 agcgtatgac aaactttttt tacccggagc atcagcttgc agaatgattg tgtgaaatat
     3781 taaaaaaata ttaacttggc taaacctttt atttacaaag atttagccaa gtttttttca
     3841 gtttggacaa gcaaatttct ttcatttttg acaaagtgac tacatgatgt taacatagtg
     3901 tggtcacttt tttcagaaag aaaaatgaat ttacataaaa aaggagaatt tgtttggtaa
     3961 aagtagaagt aaaacattta acaaagatat ttggtaaaaa gacacaagcc gctttagata
     4021 tgatgaatga tcaccagccg aaaacggaga ttttaaagaa aaccggtgca acggttggtg
     4081 tgtatgacgt gaattttgac gtaaaagaag gagaaatctt cgtgatcatg gggctttccg
     4141 ggagtgggaa atccacattg atccgtctat tgaatcgtct gattgagcca acttcgggca
     4201 gtatctatat tgatggagaa gatgtatcga agttaagcaa agaagaattg cgagaagtcc
     4261 gtcgtcataa aatcaatatg gttttccaaa acttcggtct gtttccacat cgaacgatct
     4321 tggaaaatac agaatacggg ttagaagtcc gtggtgtacc taaagaagaa cgacaagaaa
     4381 aagcagaaaa agcattagaa aattctagtt tgctttcatt taaagaccaa tatcctagtc
     4441 agttatctgg tgggatgcaa caacgggttg gcctagccag agctttagca aatgatcctg
     4501 agatcttgct gatggacgaa gcgttctcgg ctcttgatcc gctgatccgg cgagaaatgc
     4561 aagacgaatt actggacctg caagccaatg ttcaaaaaac gattattttc attacccatg
     4621 atttgaatga agctttacgg attggggaca gaatcgcctt gatgaaagac ggcgaaatca
     4681 tgcagatcgg aacaggtgaa gaaatattga ccaatccagc aaatgattat gtacgtgaat
     4741 ttgtggaaga agtcgatcgt tccaaagtgc ttacggctca aaacatcatg gtacctgcat
     4801 tgacgacgaa tatcgaatct gatggaccaa acgttgcatt gacaaggatg cgtaatgaag
     4861 aagtcagcat gctgatggct gttgacagaa agagacacct gaaagggatc atcactgcag
     4921 atcaagcgtt agaagctagg aagcaaaaac gtccattgat cgacttccta gatgaaaatg
     4981 tgacggtgat tgggaaagac atggtggtaa gtgatatttt caatattatt tatgactcac
     5041 cgactccact tgcagttgta gaggatggaa aattaaaagg tgtcgtcatc cgaggtagtg
     5101 tgattgaagc attagcagaa acaagcgagg tgagtgaaca tgaataatct attgaactat
     5161 caattaccag tggcaaactg ggtagaaaat atcacagaat ggtttacgac aactttttct
     5221 ggcctatttt catttttaca aacgattgga caggcagtga tgtcggggat tacaaattta
     5281 ctgttagtga ttccagctcc tttgtttatc ttgttattga cggtagcagc tttctttatt
     5341 tctaagaaac gtccaggact tacgttgttc acattaatcg gtctatggtt tatttataac
     5401 caaggtctat ggaatgattt gatgaatact gtcacacttg ttcttttatc tagtgtgatc
     5461 tcgatcatca ttggggtacc attagggatc ttgatggcaa aaagcagtaa agcccaaagt
     5521 attatcaaac cgatccttga ctttatgcag acaatgccag gattcgttta tttgattcca
     5581 gcagttgcat ttttcggtat cgggatggtt ccaggggtat ttgcctcagt catctttgct
     5641 ctgccaccaa ccgttcgttt tacgaattta gggattcgac aagttcctaa agaattagta
     5701 gaagcttctg attcattcgg gagcacaagt cgtcaaaaac tattcaaact agaattgcct
     5761 ttagctaaaa gtacgatcat ggcagggatc aatcaaacaa caatgctgtc actttcaatg
     5821 gtcgttattg cctctatgat cggtgcaccc ggcttaggac gcggcgtatt atcagcattg
     5881 caaagagcac aagtcgggaa tggatttgtc aacggggttg cattagttat cttagcaatc
     5941 atcattgacc gttttacaca gcatttgaac cagccaaaca ataaaaaagc tgcaggagca
     6001 acaacacaga aaagtaaaaa acgtcaagga ctgatcattg gtgctgtagt agtagtgatc
     6061 ttaggtgcga ttggtatagg gagtttttca tctgccaaag aaacaaaacg tatcaactta
     6121 tcttatgttg aatgggatac agaagttgct tcaacaaatg tagtcggtga agtattgaaa
     6181 caaatgggct atgatgtaac gatgacgcca ttagataatt caatcatgtg gaaatctgtt
     6241 tcaaatggcg agtcagatgc catggtttca gcttggctac ctaaaactca tggttcacaa
     6301 tatgcacaat ataaagatca agtcgaagac ctaggtgcca atttgacagg agcaaaagtt
     6361 ggtttagcag tgccagccta catggatgta aattcgatcg atgagttaac cgatcaggca
     6421 ggtaagaaaa tcataggaat cgagccagga gctggtgtag ttacggctgc tgaaaacacg
     6481 atccaaaaat atgacaactt gaaagactgg aaagtagaga catcttcttc aggagcaatg
     6541 accgttgcac taggtcaagc gatcaaaaaa cacgaaccaa ttgtagttac aggatggaca
     6601 cctcattgga tgtttgccaa atatgatctg aaatacttag aagatcctga aaacggaatg
     6661 ggaagcgaag agcagatcca tacgatggtt cgaaaaggat tgaaagaaga ccagccagaa
     6721 gcttacaaag tattagacaa tttccattgg tcagaaaaag atatggaaaa agttatgttg
     6781 gaaatcaaca atggaaaaga tccacagcaa gcagcaaaag actggatcaa agagaaccaa
     6841 gaattagtcg aaagctggaa aaaataaaaa gacagacacg aaataaatgg agcttccaaa
     6901 attggatcaa atatccaaat ttggaagctc catgttcgtc tctatctttt ttgtctataa
     6961 aaaaagagaa tgcgtagctc atcttcgaaa cgtccaaaga cgccgacaaa ctattatgaa
     7021 tttatgggtg cgagtcaaag agatcgatcg taggtatgaa agactaatgt gattaaagtt
     7081 tattatgtac gaagtgcatt ctcaaaaaat attaaagaat atacatagtt tatatactta
     7141 aaagtgatta aattcttatt caggaattat tttgctacgt ttcaagaagg aattgttttt
     7201 tgaaatttaa tgaatgttag aataatagtc taaaaacatt aatttagggg tgattagatt
     7261 atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg gggtaccact
     7321 catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg tacggtcgat
     7381 tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt aggtggagca
     7441 attccaggga agtgctaaaa tgaaaaaaaa tgctaagcaa attgttcatg aattatataa
     7501 tgatatatct ataagtaaag atcctaaata ttctgatatt cttgaggttt tacaaaaggt
     7561 atatttaaaa ttagaaaaac aaaaatatga attagatccc ggtcctttaa taaatagatt
     7621 ggtgaattat ctatatttta ctgcttatac taataaaata agattcactg aatatcaaga
     7681 ggaattaata agaaatttga gtgaaattgg aagaactgct g
//
LOCUS       DANJJW010000119         7610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000119 DANJJW010000000
VERSION     DANJJW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..179)
                     /locus_tag="PWS82_002442"
     CDS             complement(<1..179)
                     /locus_tag="PWS82_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0907523.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="PWS82_002443"
     CDS             complement(192..1295)
                     /locus_tag="PWS82_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL0907524.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="PWS82_002444"
     CDS             complement(1385..2341)
                     /locus_tag="PWS82_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL0907525.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="PWS82_002445"
     CDS             2622..4556
                     /locus_tag="PWS82_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0907526.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="PWS82_002446"
     CDS             5013..5429
                     /locus_tag="PWS82_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0907527.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="PWS82_002447"
     CDS             5443..6009
                     /locus_tag="PWS82_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL0907528.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="PWS82_002448"
     CDS             6032..7018
                     /locus_tag="PWS82_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0907529.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7610)
                     /gene="scm"
                     /locus_tag="PWS82_002449"
     CDS             complement(7173..>7610)
                     /gene="scm"
                     /locus_tag="PWS82_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0907530.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2184 a         1458 c         1567 g         2401 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgttgtggt agatgtatcc gtactgtcgc ttgaagctga cgtgtccgtg
     7561 ctatcacttg tggtagaggt agtatctgta ctgtcacttg aagctgatgt
//
LOCUS       DANJJW010000120         7373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000120 DANJJW010000000
VERSION     DANJJW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..531)
                     /locus_tag="PWS82_002450"
     CDS             complement(<1..531)
                     /locus_tag="PWS82_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0907531.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDES"
     gene            complement(531..1667)
                     /locus_tag="PWS82_002451"
     CDS             complement(531..1667)
                     /locus_tag="PWS82_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0907532.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1699..3150)
                     /locus_tag="PWS82_002452"
     CDS             complement(1699..3150)
                     /locus_tag="PWS82_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0907533.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3194..3958)
                     /locus_tag="PWS82_002453"
     CDS             complement(3194..3958)
                     /locus_tag="PWS82_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL0907534.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3986..4684)
                     /locus_tag="PWS82_002454"
     CDS             complement(3986..4684)
                     /locus_tag="PWS82_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL0907535.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4696..5475)
                     /locus_tag="PWS82_002455"
     CDS             complement(4696..5475)
                     /locus_tag="PWS82_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL0907536.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5491..6435)
                     /locus_tag="PWS82_002456"
     CDS             complement(5491..6435)
                     /locus_tag="PWS82_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0907537.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6481..7260)
                     /locus_tag="PWS82_002457"
     CDS             complement(6481..7260)
                     /locus_tag="PWS82_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907538.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2045 a         1575 c         1147 g         2606 t
ORIGIN      
        1 actttcatca taataaaatt ttttagcatc cactgcattt ggaatcaaag tgaatttatc
       61 atttcgaaac aaaaaatcac ctgctacttt tccacatgaa aattcatgtg tcaaaacaga
      121 cgttaactta catcgataat attgctttaa aagatattta aaatctctat ctatcgatgt
      181 attatggcta tgtgctatcc gtatcggaat cccagctttt tttgcagctt ttaaaggtaa
      241 ataactcatc gcatcaatat gagaatgaac aatctcatat tctggatgat ccttaaaaaa
      301 ttgttccatc catttaaaat atttaggata attttgtgga taaagtctcg gtgcataata
      361 gacctttcct ccaagactta ttatttcctc atcataatca cttttatatg gacgatgtgt
      421 caaaaaatca aactgaattt tagttcgatc tatattccta taatagttca ttagcatcgt
      481 ctccaaccct gcacgatgca tatcattcac acactgtaat attcgaatca ttatatattt
      541 cccctataca aatttaatgt ttcttgtacg attttatccc aattatattt actacaaata
      601 aactcagtag cttgattctt caaaacttta accatttccg attgattgca tgcctcttct
      661 aatcttatct ttaaatcact tacatccgat ttcttaaaaa tcatagcctt gtcttctact
      721 acttccgtac actctgagat atttgaaact atacaacagt tcccataact cattgcttct
      781 aacaaactta atggcattcc ttctaaatca cttggaagtg tatagatata cgcattacta
      841 tataattctt ctaattcctg tccttgtaca aatccagtaa atataattcg ctcatcacct
      901 tttgctaatt cctttagttc atttgcaaat tcatctgtat cagagcttcc tccggctatt
      961 ataagttttt tatctgtctg aacatcttta aatgcctcaa ttagataccg aattcctttt
     1021 tcgggaacca atcgccccaa aaacaaaata taagaatctt ttgttaatcc atacttttct
     1081 gtaatcaatc ctgctgattt tatttcaatt tttttaacgc catttggtat aaaccttgtt
     1141 tttcttccat atatatcttc aaaataattt tggacgcttt cactaagtac aattatttca
     1201 tctgcaaatt ttacagcaca cttttcccct aacatgatgt atgtactagc aagcttgttc
     1261 catttttctc tttgatgatc tagaccatga attgttgcaa cacatctttt cccaaaaagt
     1321 tttggcaacc acaacatagc acatggtcct tctgcatgaa aatgaacaac atcatatttt
     1381 ccgaatgctg ctctaattcc accaaatact gaagaactca ttgctgcaat tccttttata
     1441 tttaatgtaa aaatagtctt taattttaca cctttatatt ctttattttt atgctggtca
     1501 aattcctttc cgctaacatg atgaccactt cgattatagc aagtcacttc atgcccaagt
     1561 gcaaccattc gcgttgccaa ttcttctacc actatctcaa ttccaccttc tcttgatgga
     1621 attcttttat gacctagcat acagattttt aatttttttc ttgccatcac gttttactcc
     1681 ttctatagta tctttatctt acatggaacc gtttctatga agcacagttt tgactgtcct
     1741 aaacaaaatt ctaaaatcta atcccatact ccaattcctg atatattctg catcaagttt
     1801 tacaacctct tcgaagtctg tgatatcact tctaccactc acctgccaca ttccagtaat
     1861 cccaggtttc atcgcaattc tcgcacgatg atgaaaatca tacttctcat attctgactt
     1921 caagcaaggt ctaaacccca ccaggctcat ttttcccatt aggacattat aaaattgtgg
     1981 aaactcatcc aatgatgtag atcgaataaa ttgtccaatt cctgtcttct ttgttccatc
     2041 aggaagtatt ttattgccaa tgattcttgg gtcaaaatcc atcttaaaca tgagattatt
     2101 actcatctta ttttctttca tcaactctgc tttgcgttct tctgcatcca tatacatact
     2161 acggaactta tacatcttaa atgtcttacc attttgacca atacgttctt gtgcaaagaa
     2221 aatcggccct ggtgatttga tgtaaataac tggtgctacg aagataaaaa taacacctgt
     2281 tgcaatacat ccaataattc cagcaagaat gtcaatcgtt ctctttataa atgcttgcct
     2341 catcgtcata acattaatac tagatgtaat aacagtataa tcaccaactc ggccaataaa
     2401 ttgttttcca cgtgtactat cagacacttt tgtaagattc atatgtacag caagtcccat
     2461 ttccgaacat ctggcgatta attctttggg atatggaaca ctttcgtcaa cattgatgaa
     2521 tacttcatct acccaattct gtaaaagata ttccgctgca ttatcagcat ttgcaactac
     2581 tgggacacct ccgatggtag ttccatacat atctctgtct actacaatca caccactaag
     2641 tttatacatg ccataattct tctctttaag attacgaact acattttttg caatcttttg
     2701 agatgttaca ataactaacg atacatcttc accgtttttc atgcggtgat gcaacaaccc
     2761 tttccaaagt atacgaatta tatatgttaa taatccatac accaccccca ttataaataa
     2821 agaaactctg gaataatcat ttccatcctg cattgtaata aggtataaac tgcacacaag
     2881 taacagcata aaagattgct ttatagaagc tacagcctct ttataatatc ctctttttaa
     2941 aacattttta tatgcctcaa ataaaaatat aatcgtaaga ttctcaagtt ctataaaaat
     3001 tgccatgtgg cgataaatag gaatcacata cggattccag tcaccctgtc gcattacata
     3061 tgatatataa aaagcaagct ggacacaaaa taagtctaat aaaataaaat caatatgttt
     3121 agaccaacca cttgagtttt ttctatacat ctctcactct ctcctcattt cctttttatc
     3181 tacgaataaa gaactaaaat aaaccaaact tcttcttttt tacctcttca tagattggat
     3241 aattgatctc atccccgttg atcagatcct ttgcgacttg tttcatctgt actacttttt
     3301 ccttaccaaa ctccttggca atctgctcat agcactcttt taagtaaaag gtgcgcttag
     3361 agaccccgtg tgcatcagaa gcagccatct gcacgagatt atgtttgacc attgttttgg
     3421 ctgttttctg gatatctttg ccaaatacac caacataact tggcgcagta agttgggcca
     3481 aacatcccat atctaggaac ggaagcaaat gatttgggtc ttctcgaaaa tgcgcgttgc
     3541 gttctggatg gacgatcact ggtatctttc ctagttctaa caactcaaaa aaaagccgtt
     3601 ctgcatataa cggcacttcc agtgtcggaa attcgatcag aagatatgta tcatctaaat
     3661 ctgtaaataa aatttcatct cggtggatgt cttcgatgag cgttccggta atacgtactt
     3721 cttgtccttc caataacgtc aaaggcaact gtcttttctc taattcggct tgtaacgaag
     3781 cgactagaga aatgacttgt gatttttcat tgctgtattt tccattattg tggtgtggtg
     3841 tgcataaaat atgcgtaatg ccttgctgaa tcgctttttc agccattgca atacttgctt
     3901 ctaagttttc tgccccgtca tcgattcctg gtaggatatg gcaatgtaga tcgatcattt
     3961 ctcttgtctc ctctcttctt attccttaat ttccgtagta ataaccagca tctttgctgt
     4021 gttctgctcc gttgtatacg acacctaaga ctcgtgcttg taccatatct aacagttctt
     4081 ttgctttagt caatgattct tttcgtgacg tattttcccg tacgaccaat aaggtgccat
     4141 ccgtttttga agacatgatc tgtgcatctg ttacggcaac gactggcggc atgtcgaaga
     4201 tcactacatc aaatagttgt cttgcttctg ctaaaatctg atccattctt ggcgaactta
     4261 gtagttctga tggatttggt ggtttaggtc cacttggcag aatcgacaaa ttgtcgactg
     4321 gtgttcgttg gatctcatct gctacacttc ctgatgaact aagaacagta cttaatccgc
     4381 ttgcgttgtt caacttgaac gttttataga tgacgggctt acgtaagtct gcatcgacta
     4441 gcagcacccg ttgaccagat ttggcaaaaa caacggcaat gtttgctgca gtggtcgatt
     4501 tcccttctcc aggtcctgat gaagtcacaa caatcgtttt gatttgctga ccagctgcgg
     4561 aggcaaactg gatattcgtc cgaatcgtac gatattgttc agcgattggt gaagagggat
     4621 tggtgagtgt gaccaaactg actgcatgag tttgggctgc tttttgtttc tgtgttcgtg
     4681 ccattttctt tcctcctaga ctcttgagcg gcttctgcgt gatggcgctg ttttatcgtt
     4741 tgtagctttt ggttgtgtct gagctggtcc ttttttacgg ataccagcat tcaattcttt
     4801 cgctgacatt tctggaactg ttcctaagat tgggaatcct aatgtatctg tcacatattt
     4861 ttcatctttc actgtccgat cgagtaattc caataagaat gccagaccta cacctaccat
     4921 cattcctaac attaagccag tcattaaatt caatttatta tttggcgaaa caggtgtcgt
     4981 attggcaact gcacctgaaa tgatcgagat tttatcaaca ttcatcacat cttttgcgtt
     5041 ctcttggaaa acttgtgaag ttgtgttagc gatctgttga gcagccactg cattcgtact
     5101 tattgcctga atcgagaaca tttgtgaatt ttctgcctga ttaactgaga tggcatcttt
     5161 gatttgttca gcagtcattt tcacattgtc ttctgtttct aaacgatctt tgacttcatt
     5221 gagcactaaa tcactgacga tcatatcttt atacgtattg atcatttgca ggttcgtatt
     5281 gacatcattg acatttgcag tgtctgattg tggcagtgtc acgatcaatt gtgcttgtga
     5341 actgtatttt ggtgtcaaga tgaaaaatgt tacgattcct gagatggcca agccagcaaa
     5401 cattgagatg atgattatta ccaagtgttt gcgtaatgtt tcaaaaattt ctttcaagct
     5461 gattgtctct tccatttttt ctccactttc ctatttattc tctagattca tttgatcttt
     5521 taattctttt ttcacacgat caagttcttg atcactgact ttttgatagg agatattatc
     5581 ttgcatgaag ccttctcctt gcagctgatc ttgcttaatc gtaccaaatg cctcccgata
     5641 atctaaagca atttttttca tttcatcaaa gttcatgtct gttttcatat tcgactctag
     5701 agcattcaaa acggattgat agttgctgac accgtttaga ctcaatactt ttttcgctac
     5761 gccttctacg atctttcttt ggcgcccttg tctaccgtaa tcaccatttg ggtcttcata
     5821 acgcatacga gaataagata atgcctgatc cccgttcaat gtaattttac cgattggaaa
     5881 gtcatagcca tcttgtgaga aagtgaggtc attattgact tcgataccac caactgcatc
     5941 aacgagttcc tttagccctt tcatattgat agagacatag tgatcgattg ggatgtctac
     6001 atatttttgt acggtatcca tcgccatggc agctccgccg aatgcgtaag cgtggttgat
     6061 cttgtctttt gtgtcacgac ctacgatgtc gacatacgta tcacgaggaa tgctgacgat
     6121 cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc atcgtatctg atcttccttg
     6181 atcgacccga cctaaatctc cagtatcaat ccccatcaat agaacagaga aagattcttg
     6241 tttgtccaga tcaactggtt tcgttcgttc tccttgggtt cgttccactg attcgtaagt
     6301 tttctcaata gaatgtgaga gatccagata taattttgct cctactccta caattgctaa
     6361 tactaatacg ataagaactg ctaaaataat cagaaatatt tttttccctc ttgattttct
     6421 tcttcgtctt gccatagttt cctccgtttg ctaaccttgc ttgaatcaac gttttaactg
     6481 tcaatagctt atattgctat agcccatttg ctcagcaaca gtttgtgctt ttgttgcgta
     6541 tgctgcgctt attttttcat tccggatctg ctctatttga ctgagcaccg tcaaatattg
     6601 atcgtaagta gcggtggcag tcacttgacc atcctttaac atcgtatcga ataactgatc
     6661 tagttgcgtg acttgcgcga tctgaatatc agcgaatcct agatattgca taacaaccgt
     6721 tttccaagga ctatctttaa aaaagctcat attttcacgt acacgagcga catctgcact
     6781 tgtcaccttt tctttgatga tgacatcgtc tacagcttgc tgccagtttg atacattttt
     6841 agtaaataaa tcgttgacag ctgtctggat cttcagtttt gattctgcac tatctaactg
     6901 tgtcacgatt gctttcttct catcttggat tgcaacagct tctttaggca attctgattc
     6961 tttgatgtcg taatcatctg ctgaaagttt gacagaggat agcttatttg acaaacttgt
     7021 gattgtttct tctgttattt cagggtttag aaaaattttt tcttttgtac tatacagctt
     7081 gtctacttct tttgataaag aagtcaaggt gtctttttga ttggtgatgg cagattctgc
     7141 ataattgatc cgttgctgat gttgccgctg attattgata tggtatccaa acaaacagat
     7201 cgcaatagca cctgcaccta ccgccacagc agtcaatgac ttgacgaatg gcttttccat
     7261 agattcactc ctaagtattg atgatagata aattaagata taattcactt acataaaaat
     7321 gacagctttt tatttaaaaa aacataatta aactaatata ttcacagaaa tat
//
LOCUS       DANJJW010000121         7189 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000121 DANJJW010000000
VERSION     DANJJW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            20..1030
                     /locus_tag="PWS82_002458"
     CDS             20..1030
                     /locus_tag="PWS82_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0907539.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1099..2043)
                     /locus_tag="PWS82_002459"
     CDS             complement(1099..2043)
                     /locus_tag="PWS82_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0907540.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2027..4747)
                     /locus_tag="PWS82_002460"
     CDS             complement(2027..4747)
                     /locus_tag="PWS82_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0907541.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4744..5964)
                     /locus_tag="PWS82_002461"
     CDS             complement(4744..5964)
                     /locus_tag="PWS82_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL0907542.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6245..7090)
                     /locus_tag="PWS82_002462"
     CDS             complement(6245..7090)
                     /locus_tag="PWS82_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0907543.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2123 a         1371 c         1145 g         2550 t
ORIGIN      
        1 tacaggagtg caaattttaa tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt
       61 ggctgtttta actctagcag cttgttcagg cgatactaat aaagatatcg caacgatgaa
      121 aggcggaacg atcactgtat ctgatttcta tgatgaagca aaacttgaat catcgaacca
      181 atccttagta caacggatga tcatttataa agtattcaat aacaaatatg gcgataaagt
      241 aaccgataag caagtggatg cagaatacga caaacaagcg aaatcattag gtgatacttt
      301 tgaatcacaa ttagaagctg ctggatatac gaaagatact tataaagaat atattcgtaa
      361 caatttagct tttgaagctg gcttgaaagc tcacgtagat attacagatg acgatttgaa
      421 aactgcttgg aaatctttcc atccagaagt agaagcacaa atcatcaaat tatcaagtga
      481 agatgaagca aaagatgtga aaaaatcagc ggatgatgga gatgatttct ctaaattagc
      541 caaagacaaa tcaactgata cagagacaaa agaagatggc ggtaaagtta aattcgactc
      601 aacgacaaca actattcctg cagaagtaaa agaagcagct ttcaaattga aagacggtga
      661 gatctccgat gttattacta caacaaatcc tacttcttat gcgacagaat actacgttgt
      721 caaaatggtg aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag atcaactaaa
      781 agatatcgct actgaaacta aactaagtga caatgcattc acaactaaag taatcggtga
      841 agaattaaaa gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc
      901 attcacaaca acttcatcaa gtacgaaaga ctcaagtgaa actacagctt caacaaagag
      961 ttctgacaca aaatcaactg attcaacaaa agaatcttca actgaagaaa caactgattc
     1021 atcaaaataa aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt tgttttgaat
     1081 gaaaaaaata aatttttgct ataaatcttt tggtacatag aaacttcggt tgtccatacc
     1141 gaaaatccga tcggtgtatt ttcctggttc tgtttgacga atcgaagtca acatcatctg
     1201 catcgaagca tcgatattgt cgatttgatg taatatttct gcttccatta ttcttggacg
     1261 tacaggtgag ccatattcta ataagccatg gtgtgctaaa accatatgac gtaatacaag
     1321 aacatcttcg tcagcttcgc tgattttcaa cgcaagacag gctttagtta tttcttcgtc
     1381 aatcaatacc agatgaccga tcaaatttcc tgcaagcgta tattctgtcg acataggacc
     1441 ggaaagttca atcacctttc ctaaatcatg caaaatgata ccagcataca acaatgctcg
     1501 attcagctca ggatattcat cggcgattga tttccctaag tgaagcattg tcaaagtatg
     1561 ataagctaat ccactggcaa atgcatgatg gtttcttttt gctgccggaa actcaaaaaa
     1621 ttgtttttga tatttattaa gtagatagcg aacaatacga ttccaatgcg cattggtgat
     1681 ctcaaacaac aattgattga tttcttcttc catttcttct ttcttcaaag gggctctttc
     1741 catgtataaa ctaggctctc ctggctcatc tacccttgtt aagcgcatat gaagaatctt
     1801 tacttgaggg tttccttgat aaacctctct tttaccgttt agaaaaacga ctgtgccggc
     1861 tgtaaattta ttgatttcct cctccgaagc atcccagtat ttaccatcga tagtacctga
     1921 agtatcttgg aaagtaaaag caataaattt ttttccgttt ttagcgattc ttacatctgc
     1981 acttttgatc aggacaaatt gttcaaactg ctcatcgatt actaaatcac gaattttttt
     2041 cattatttgc tccttctaat tgttttacga tttggttcat ctcatggtaa tagcttacca
     2101 tttctttatc agaagaaaaa caaattattt gatacttttc tccaaattta gctaaaagtt
     2161 cagctaattg atactttcgc tgatgatcat aatgaagcca gccatcatca atgatgatcg
     2221 ggcatatcga acggttagct tctaatgaga ggtaagcaaa tctcatcgcc ataatcagct
     2281 gatctttcgt acctgtagat aactcataga tgggaaattc tgtatatccg tctgttaaag
     2341 tcagtgtatc atttttcagt aatatcttct gataatttcc ttttgttaaa atacctaaat
     2401 aatcacctgc tgctcttaac aactggggta attgtctttc actcatctct gttgaaagat
     2461 cacctaagaa tgtcccggcg atttcaagtg ctccccattc ctccagtaat tcctgtaatt
     2521 ttgctttttg ccggctagct tgttgataaa gttcgtccaa tgtgccgtct ctttgcaact
     2581 cttctacttg aagttggagt cgttgccgat tttcacttgc ttctttttct tcttgctgga
     2641 gttgggattg cttttctttc agttggcgca gtcttacaga taatttctct tttgtcagct
     2701 cttcaggaaa tagtggagta agcatttccg ataattcatt gtatcttttc atctgctgtt
     2761 cggcttgttt ggctttttgt aaaaagacag gtacttccga aggataagat aagcctgctt
     2821 gagtcaattg ttcgctgtat tcttgtatcc atactttttg tttctttttc aattgattga
     2881 tttcttgctt caataaagta ttttgctgat agcttctggc aaatttcatc tgttccatct
     2941 cttgtataaa acgggaaagc aataccattt tttctgaaat tgtcttatct tgtatcggta
     3001 accattcttt aacaaactca aatttttggt cgatttgtcg tagtttttct tgtatttcct
     3061 gttctctttt atgtttccgc tcaaatacgc catataattt ctgataatct atgacttcct
     3121 gtccatattc tttcatgagt ttgagcgtat ccatctttcc taagtgccga tgatccgcaa
     3181 aacgatatac ttcttgctga agaatctgtt tttcttctcc agttctcttt tgtttttctt
     3241 ctgcttcagc taattgagct aaggctagat caagttgtcc tagtttttct tgccacattc
     3301 cttttatctc agagaaatca tttttagttt taatttttgg ttgccttttg tagaagaaat
     3361 agccaatcat gcctgcacca attagtaaaa ggatcacttt caagggcatt ggtacaaata
     3421 tacctgcaat gattattgct ccgccagcca tcagcatata cggtttagat gaagagactg
     3481 gtgctatctc tgactgattt tgtaataatg ctggataggt ctcttccaag tcattcactt
     3541 ctgcttcaat catctcttgc tgttctttag ctatttggag acgcacagaa atctggcttt
     3601 ctagatcagc aatccttctt tcttcagcca gaagatgctt ccattcttct tcatataaaa
     3661 attcttccgg taacggattt ttctgccaat gccacctttg ttccaaaatc ttcaattctg
     3721 aatttgtttc tctagtctca atttgtaatc tttgccattc ttcgtttaat tgttccgctg
     3781 taaattgaag ttgctggaga tcttttagtt cctgctcctg ctctaaataa aaataatatc
     3841 tggctgactg ctgatccatc ccgctatgct tttccagtgt tgcttctaat tcttccaaac
     3901 gctcatttgt ttgctgatac cgtttggaga aagactccag atctaaaaga agttgctcat
     3961 caggaagacc tgtagtgctg ttttttttga gagattgcca ttcttcatat aaagaaaaat
     4021 taagttcttg ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat tgtgccaata
     4081 actgctgctg ttgcgactcg tattcctgtt cttgttggat caacttgcca aaataagctt
     4141 cctgttcttg tttttgatga attttttctt gcagctcttt ccattcattt aatttttgat
     4201 tgattagtaa tttttggcct tttttcttga ataattgttg tgctttctga taatattctt
     4261 gccgcttttg gaatacttga gaactgcccg tgatgcctaa agaaatcagc gcatcatgta
     4321 gctctttttc tctcaaatga tctagttcca tcaactgctc ttgctggaaa gtaaatacct
     4381 gctggaataa ctctttcgtc aaaggtgcga tcagtttttc tagtaactca tcacttccca
     4441 cttgatcccc cagccacacc ttcgattttc cgcgattttg ctgcttgtag cgctcgacag
     4501 caaattcacc atatacagga tgtttaagcc atattttgcc gccataggct gagccgtctt
     4561 ttggtgtata atcctttttt ttacggcctt ttgaagggaa accaaataat attgcttgaa
     4621 taaattgata aatcgttgat tttcctgctt cgtttccccc atagatcagc tgattgcctg
     4681 aataaaagtc aaaacttttt tgacgccatt ttccaaaagc agcaatctcg atccttaaaa
     4741 tcttcattcc tgatcctcct caaataaaaa atcttgatta agcagctgct tggctttttc
     4801 aagtgtttct tcttgatatt ccggcagttc gctcaatatt ctagccgctt cttgatgact
     4861 atatacttca tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt gaaataactg
     4921 ttccatcaat ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat tctctatgag
     4981 agaaatacgc caaataaaga aatcatttat cctatctgag aattcctcta acaaatagtc
     5041 taatagctct cctgattgta tccgttccaa aacatctgta ttgagatggt cataatcttt
     5101 taattttatc tcaattaaac taaatccatc ctctactttt gaaagctgct gacgaatgtg
     5161 aacaatgacc tcttttgtcg ttcttgcttt cctcaatgat atttcctttg tcttccaata
     5221 gacctctgct actttaatcg gatgtatttg gcaacttcct ttacttaatt caactagtag
     5281 aatagatgta gaagcttgtt cctttttagt atgaccttga ggcgcacctg gataaacaat
     5341 tgtttgcttt tcatttaaga ccgttggcac atggatatgc cctaaagccc aataatcgta
     5401 tcccttttct tgcatctttg agggttggaa aggagcatag tttccttttt gagagacgcc
     5461 aggttccccg tggtacaacc caatatggta atctgtgagt tctctagaag gaaattccat
     5521 cactttgtct ttttgaatcc acggttgacg ataactaaat ccactgagag agacagtttc
     5581 tccacttttt atcgttattt tttttgtctc taccgtttcg gatgtaaata aatggacatt
     5641 tttaggaaag gagaaccaat accgctcttt ttgatagaaa tcatgatttc cgaatattat
     5701 aaatacaggt atatggttct tttctaaccg tttcatctgt tctgaaaaat gtttctgtat
     5761 ttttaatgaa ggacgatttt gatgaaaatt atctcctgca agtaaaacaa aatcaacgtt
     5821 atttattatt gcctgttcaa tgatatttga taaaacggtc agattcgtct tcaatagttt
     5881 ttcctgtaca gttttatcaa gagttgtcag tccttcaaat gaccgatcca tatgtaaatc
     5941 tgccgcatgg ataaatttca ccattaaaaa tttcctcctt tttttctttt atcataccat
     6001 tttcaatgtt ttttttcatc aataaccgaa aatttgttcg tattatgcaa agaaattacg
     6061 ataaatggcc gacttcaaaa aataagttta ttcgaaaaaa agattgtgac atgatttttg
     6121 tcacaatcct aaatatctat gttatattcg cttcgtttga cactgaagaa gtactatttg
     6181 tactggaatt tatgctccat ttttccgtat ttagaattct atgttctata ctaccttaat
     6241 tagtttatat ggtaagaatt ctttcaaatg cttgagatac tccgtcttta tcatttgaaa
     6301 cagtgacata atcagctttt ttcatgattt cttgatgact atttcccatt gcaaaactaa
     6361 tatctgcact ttcaaacata gataagtcat ttaaactatc tccgatcgcg attctcaaag
     6421 gtttctttaa attgtagatt gattccaaac gatcaaacac cacaccttta gatacatttt
     6481 gaggtacgat ttcaatattt acaggtgccg attcagtaac agacacattt agcatctttt
     6541 tcaaatggtt aaatatatta gctttcaatt gacgattagg aataaaaata aaaaatttgt
     6601 aaatgctgat agcatccttt tctataattt cctcaatatt attataaggg attgaacaaa
     6661 tagatttttc atactcaagc tcatagttta gctctttgat agaaaattca ctccctacct
     6721 cgtgaaaaat ttttttcatt ttatttttat aattctgaga ttcataactt ccgagatttg
     6781 tatatatttt ggttggaaat ttcgatagga cagaatcaat tttttttaca tcatttttat
     6841 cgaaggaaga ctcaaatatc tttttttctc catcaaatcc aacagaacca tttaacccta
     6901 ctgctggtat ccttaaacca gattttttga gaatattatt aatgtcttct acttctcttc
     6961 cagaacaaat gaatggaaaa cagtcttccg gtaaatgttt cattgttctt gcattttttt
     7021 gactaatgct ctggtcagaa ttcaaaagag taccgtctaa atctatacca attagtttca
     7081 tttcatccat gtctcctata cgttgattat aaattcactg cttattgtaa gtctaaattt
     7141 ctcacaaaac atatactaat tatacctaat ttaagtaagt acaaacatc
//
LOCUS       DANJJW010000122         6924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000122 DANJJW010000000
VERSION     DANJJW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            94..1248
                     /locus_tag="PWS82_002463"
     CDS             94..1248
                     /locus_tag="PWS82_002463"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL0907544.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="PWS82_002464"
     CDS             complement(1270..2022)
                     /locus_tag="PWS82_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0907545.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="PWS82_002465"
     CDS             complement(2386..5052)
                     /locus_tag="PWS82_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0907546.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="PWS82_002466"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="PWS82_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0907547.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2044 a         1421 c         1252 g         2207 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DANJJW010000123         6898 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000123 DANJJW010000000
VERSION     DANJJW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(29..487)
                     /locus_tag="PWS82_002467"
     CDS             complement(29..487)
                     /locus_tag="PWS82_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0907548.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(500..1831)
                     /locus_tag="PWS82_002468"
     CDS             complement(500..1831)
                     /locus_tag="PWS82_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL0907549.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1842..3284)
                     /locus_tag="PWS82_002469"
     CDS             complement(1842..3284)
                     /locus_tag="PWS82_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0907550.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3271..4035)
                     /locus_tag="PWS82_002470"
     CDS             complement(3271..4035)
                     /locus_tag="PWS82_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL0907551.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4139..5014)
                     /locus_tag="PWS82_002471"
     CDS             complement(4139..5014)
                     /locus_tag="PWS82_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0907552.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5269..5353)
                     /locus_tag="PWS82_002472"
     tRNA            complement(5269..5353)
                     /locus_tag="PWS82_002472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5317..5319),aa:Leu,seq:caa)
     gene            complement(5371..5442)
                     /locus_tag="PWS82_002473"
     tRNA            complement(5371..5442)
                     /locus_tag="PWS82_002473"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5408..5410),aa:Gln,seq:ttg)
     gene            complement(5452..5524)
                     /locus_tag="PWS82_002474"
     tRNA            complement(5452..5524)
                     /locus_tag="PWS82_002474"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5489..5491),aa:His,seq:gtg)
     gene            complement(5586..5670)
                     /locus_tag="PWS82_002475"
     tRNA            complement(5586..5670)
                     /locus_tag="PWS82_002475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5634..5636),aa:Leu,seq:caa)
     gene            complement(5691..5761)
                     /locus_tag="PWS82_002476"
     tRNA            complement(5691..5761)
                     /locus_tag="PWS82_002476"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5727..5729),aa:Cys,seq:gca)
     gene            complement(5807..5878)
                     /locus_tag="PWS82_002477"
     tRNA            complement(5807..5878)
                     /locus_tag="PWS82_002477"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5844..5846),aa:Gln,seq:ttg)
     gene            complement(5890..5962)
                     /locus_tag="PWS82_002478"
     tRNA            complement(5890..5962)
                     /locus_tag="PWS82_002478"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5927..5929),aa:His,seq:gtg)
     gene            complement(5965..6038)
                     /locus_tag="PWS82_002479"
     tRNA            complement(5965..6038)
                     /locus_tag="PWS82_002479"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6004..6006),aa:Trp,seq:cca)
     gene            complement(6045..6125)
                     /locus_tag="PWS82_002480"
     tRNA            complement(6045..6125)
                     /locus_tag="PWS82_002480"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6089..6091),aa:Tyr,seq:gta)
     gene            complement(6127..6202)
                     /locus_tag="PWS82_002481"
     tRNA            complement(6127..6202)
                     /locus_tag="PWS82_002481"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6167..6169),aa:Phe,seq:gaa)
     gene            complement(6238..6310)
                     /locus_tag="PWS82_002482"
     tRNA            complement(6238..6310)
                     /locus_tag="PWS82_002482"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6275..6277),aa:Asp,seq:gtc)
     gene            complement(6320..6392)
                     /locus_tag="PWS82_002483"
     tRNA            complement(6320..6392)
                     /locus_tag="PWS82_002483"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6357..6359),aa:Val,seq:tac)
     gene            complement(6408..6479)
                     /locus_tag="PWS82_002484"
     tRNA            complement(6408..6479)
                     /locus_tag="PWS82_002484"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6444..6446),aa:Glu,seq:ttc)
     gene            complement(6494..6583)
                     /locus_tag="PWS82_002485"
     tRNA            complement(6494..6583)
                     /locus_tag="PWS82_002485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6547..6549),aa:Ser,seq:gga)
     gene            complement(6623..6696)
                     /locus_tag="PWS82_002486"
     tRNA            complement(6623..6696)
                     /locus_tag="PWS82_002486"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6660..6662),aa:Asn,seq:gtt)
     gene            complement(6706..6821)
                     /gene="rrf"
                     /locus_tag="PWS82_002487"
     rRNA            complement(6706..6821)
                     /gene="rrf"
                     /locus_tag="PWS82_002487"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2054 a         1536 c         1139 g         2169 t
ORIGIN      
        1 caccctattt aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta
       61 cttcttggtt ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca
      121 ccgtatcttt ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt
      181 cttttacctc ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg
      241 tatctaaacc catatggatc agtatttttc tgccagattc actttcgatc gttatggcat
      301 gtcgggtagg aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat
      361 gattcgttat agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg
      421 ctgttaatgg aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga
      481 aatgcacttt ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca
      541 tttcatctag tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg
      601 cttgtaaagc tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg
      661 caacttgtgc ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg
      721 gctccacacc agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat
      781 tcggaatcgc cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca
      841 aggaaatagc tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt
      901 cttccacaat accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat
      961 ccatgaccat attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat
     1021 ataagtaata ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg
     1081 acatcatttg aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca
     1141 tttcttttgt tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat
     1201 cataaatcga tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct
     1261 tacgatcata accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg
     1321 cattaatcat ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg
     1381 gatttgtata attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt
     1441 ccaataaacc tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt
     1501 gtcctactaa tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc
     1561 caacacgaat ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg
     1621 ttgcacgaat gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt
     1681 acatatccat atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc
     1741 gtttttcatt gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag
     1801 ctatagtgat aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta
     1861 ttgaattctg ccataacata agtcacttct aagccgatga taatttgata atttttttca
     1921 gttggtttaa tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg
     1981 ggatccacta cctctacccg taggcgtgta ttacaactat agacatccag gatattggct
     2041 tttcctccaa acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac
     2101 cctaactctg tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct
     2161 aatgtctgaa tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa
     2221 aagaaaattc cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga
     2281 taatcaacaa tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca
     2341 atccctgcta aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg
     2401 aaactaaatt caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc
     2461 atgattcctt tgaccatctc tttattttct ttataagctg ctttatagat agctagacat
     2521 atagctggta taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga
     2581 ggatctccgt tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta
     2641 tagcttccta aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc
     2701 agcaaacgat tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt
     2761 tttgcaaaat tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct
     2821 gcgattgttt gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa
     2881 aaaactactg gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg
     2941 gaaataattc caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt
     3001 ttcaaggtag caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg
     3061 acaaaaccga ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt
     3121 ccagctgcac caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat
     3181 cccgctaatg gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt
     3241 gatttaacat tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat
     3301 caaaggatca tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg
     3361 ggtacgagga tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg
     3421 acacatgact tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc
     3481 ttccacactc gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca
     3541 aggctcaaaa taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg
     3601 caaatcatat ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt
     3661 atgatgatga ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg
     3721 agcattccat tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga
     3781 taaggatctg aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc
     3841 acacgtcaat gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac
     3901 agcatgctcg aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg
     3961 ataagcatcc ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa
     4021 aatcactttt cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa
     4081 tcatagtcca tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt
     4141 aatagattct tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt
     4201 gaaattgctg ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt
     4261 taaatgtttc agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc
     4321 gtttaaccag gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat
     4381 aaacgtttga ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt
     4441 ttaagctctt taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga
     4501 atcctacttc atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt
     4561 ctactgctat atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg
     4621 tgacatctct atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa
     4681 tatagatatt tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt
     4741 ccgcaaaatc cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct
     4801 tttctttaga atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca
     4861 ctttagcaga aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga
     4921 tcaatacagc attatgattc agctttttca tcacatacac cgttaattca cctcctctat
     4981 atgataaaac aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt
     5041 taaagacaaa aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta
     5101 gcaccctaca taatttacaa aaatagttta acacataata aaagcgttat caatatcttt
     5161 tttactcgtt ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca
     5221 aaaaaaatat agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg
     5281 gggtcgaacc ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt
     5341 ccgccacgcc ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg
     5401 agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga
     5461 tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat
     5521 tcgccatcat aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta
     5581 gtcaataccg gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc
     5641 cagcgcgtct gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac
     5701 cggatttgaa ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc
     5761 cattatacta ttatcaatct ataaaataaa gaataaatta tagaaactgg ggtagctgga
     5821 ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag
     5881 aataaaaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5941 ttaaccactt cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg
     6001 ttttggagac cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt
     6061 cgaaccactg aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact
     6121 cctccatggt ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct
     6181 ctaccaactg agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg
     6241 ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca
     6301 ccacggaacc agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa
     6361 ggcagatgct ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg
     6421 attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca
     6481 ggtaaatttg ctacggagag taagggattc gaacccttga gacagtttgg caccatctac
     6541 atgatttcca atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca
     6601 atactctttc tcaaaatgag aactccggca gtaggactcg aacctacgac atcatgatta
     6661 acagtcatgc gctactacca actgagctat gccggaataa catcagcgtg gcgacgtcct
     6721 actctcacaa ggggcaaccc ctcactacaa tcggcgctaa gaagcttaac ttctgtgttc
     6781 ggcatggtta caggtgtatc cttctcgcta tcgccaccac actgtggtgt tatcttttat
     6841 tgagtaaatt ttgttcactc aaaactggat ttgaagtcac ataagttttt ttccgagt
//
LOCUS       DANJJW010000124         6595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000124 DANJJW010000000
VERSION     DANJJW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1..1114)
                     /locus_tag="PWS82_002488"
                     /pseudo
     CDS             complement(1..1114)
                     /locus_tag="PWS82_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1357..1812
                     /gene="tnpA"
                     /locus_tag="PWS82_002489"
     CDS             1357..1812
                     /gene="tnpA"
                     /locus_tag="PWS82_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL0907553.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2077..2175
                     /locus_tag="PWS82_002490"
                     /pseudo
     CDS             2077..2175
                     /locus_tag="PWS82_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2858..3676)
                     /locus_tag="PWS82_002491"
     CDS             complement(2858..3676)
                     /locus_tag="PWS82_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0907554.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3837..4526
                     /locus_tag="PWS82_002492"
     CDS             3837..4526
                     /locus_tag="PWS82_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0907555.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4540..6042
                     /locus_tag="PWS82_002493"
     CDS             4540..6042
                     /locus_tag="PWS82_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0907556.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6055..6531
                     /locus_tag="PWS82_002494"
     CDS             6055..6531
                     /locus_tag="PWS82_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0907557.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         2127 a         1045 c         1207 g         2216 t
ORIGIN      
        1 caatcgttag tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc
       61 ttttcagaga tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga
      121 tacaatttct tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt
      181 gtatccgtcg tgattttctg atggcagata ctggtgtaga aacgacgatg gatccgattt
      241 ttggctactg ctccgacttg tcctcgataa gaattgtagc gccgtgattt acgtgtgtat
      301 gtcgttactt ttattcctag cttcttgatc aggcgctgaa ctttcttttt attgatagag
      361 aaaccgcggt ttcttagttc attggtcatc cgtaaacagc cataatcctt gtgcttctcc
      421 cgaactttaa gcatttccgc ttcaatctct tgatctggat ttttacggtc caatcgtttt
      481 tgccagtaca taaagattgc cttagggaac tttaaagttt ctagaagctc aactaattta
      541 aacggtcctc ggaggcttgc gatgattcgt gcgattgctt cattcgtcgt tgttgggctt
      601 cctgttttcg cagcttcctc aattctttta aaaaggcatt ttcaatttga agggaacgga
      661 ctcgtttctc caactcctcg atgcgttcac gttctttaga cgttgcttta gttgtagatt
      721 ttttcttatt tttgggttca tttttcttag acaagatagg tggacatcct ttcgtttttg
      781 agagtccatc gatcccaccc tcacggaatt gacgcatcca actagcgatc aaattagggt
      841 tgttcatgtt aagtgtgttg gcaacttcac gataggacag ctcacttgtt agatataact
      901 ctatcgcatc cagcttaaat tgaacagaat attttttctt ttgtcgcttt cttactagac
      961 cctcttcacc aaattcttga taggcattga tccagcgctt cacttgtgat ggagccttaa
     1021 tggaatattt ctttgccaga taatcacttc cgcctttacc atccaagtaa tcatgaacga
     1081 tctttaattt aagttcaaaa gtatacttag ccataaaaaa taccgacctc caaaagttag
     1141 attttttggt ctaacttttg ggggtcggta caaaacttaa aagttttaga cccttttatt
     1201 ttcttaatga cttcagtcag ttttgtgcgt aaagcgtgcg aaacaagaaa accgcgcgtt
     1261 tctacgcgtg gaagaagtat ctttagcgat aaaaactcct ttcgatatac tagtgagtgg
     1321 ctaccaaccg ctcatcagat cgaaaggagt tttttcatgt caaatgacga taaaagttta
     1381 gcatatactc gttggaattg taagtatcat attattttta caccaaaata tcaacgaaaa
     1441 gtaatctatg ggcaattacg aaaagatctt ggaaaaatac ttaggaagtt gtgcgaaatg
     1501 aaagatgtgg aaatcataga agcacatgca atgctagatc atattcacat gcttgttcga
     1561 gtaccaccaa aaatatgtat atccagtttt atggggtatc tgaaaggtcg tagtgcggta
     1621 ctaatccacg agcagcacgc aaatctcaaa tataaatacg gaaacaaatc cttttggtcg
     1681 aagagatact atgtgagtac agtcggattg aatcaaaaga caaatgaaaa atacatccgt
     1741 gaacaagaag ctgatgatcg cattcgatat attataagta aaagagagta cgtagaacca
     1801 tttaaaaaat aaagagaagt aagtgagtga agatgagcag ttggcggtct tttaaaaatc
     1861 cccttttaag gggcagcgag tgtcgacccc ttctaggggg attaaaaata cacccgtcct
     1921 cacgggtgta tgggttactg atagacttga ttttaactgg tgtcagaaac ttatgtccag
     1981 aaagggtgat gaaattttgg caatttacgg ctattcaaga attagcacaa gcaaccaaga
     2041 ttacaaaaca caaattcaaa cattagaaaa tgctggcgca gaaataatct tcctagaaaa
     2101 tcactggatt atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg
     2161 tctaacttaa tttgacaatt aagagtatat ttacttcaga tggtaaggca acaaaataaa
     2221 atacaatagg cagatgaact aataaataaa atctgtatta aatacagata aattaatata
     2281 aaaatcaaaa ggatcataaa cttttaacaa gtgatgaaat gggtggaaat tgattcttat
     2341 agacagtttg tctcataaca ttgaagtatt ttgcaatttt agcattgaaa cactttttta
     2401 atcttcaacg ttgttcttat aaacaagatg attgggaatc ttttgaatta acactgtata
     2461 gcttagaaca ccctttatag aaaacattcg cttagcaatc ttaataattc cttgtgcccg
     2521 atgtcgttta gattcagtag gttcctgctc atcgatcatg gaaaaaattt gaaggatcag
     2581 atccttaata aatttattta agagaggatg atccattgct tcatccatac tcgataaact
     2641 agtgataatg agctacacgt ttttctttca agttgatcaa tagcttccac gataaaaata
     2701 tctttagata aaatcaagaa ccgtaggaac tacagggata tttggtcaat aagagccacc
     2761 gctattagta aaaaataagc tatcaagtat gctctcatcc caagaattgc ctacttcaat
     2821 cgttaaaaac cgttaggttt agctaagtgg gggtagttca ctcaatactt ttatcaataa
     2881 tagagcgcaa aactttttct cttaattcaa ctgaatcaat attattgttt tgttcataac
     2941 catgaactaa tagatcacaa atataaagtt gtgatatttt tccagctaaa gatccaccat
     3001 taagaaattc ttcaatggct gtttgtaaaa ctaagtctgc agactttcct ataggtgaat
     3061 gaatatagtt agttatagca ataatttttg cgccattttt tttagctatt ttcaatgaat
     3121 cataagtatc tttcgttttc ccagataaag aaaaaatgat tactaaatca ttaactgtta
     3181 ataaagaagc gacttgggct tgaaaatgag ggtctagtac agcttttgct tgaactccta
     3241 cgcgaagaaa catattttct aaatcaagac tagtatttcc actagatccc actccaaata
     3301 tatatatgtt ttttgattga gtgatcattt taatggcttc attcaatttt tcttcgtcta
     3361 aaagcctaat agtggaatag agagcttctg ttaaactatt agcaatttca tcatagtact
     3421 ttacactagt cggatgttct tttttttgac taaagtcttc ctttgcaatt tcaattttta
     3481 aatcagaaaa accagaaaaa ccaatttttt gacaaaagcg aataatagta gcatcgccaa
     3541 cttttgtgtt tttcttaata tcactcatcg tactataaat aatagttttc ccagaattta
     3601 aaataaaatc agcgactttt ttttcagact tcgttaaaaa tggataattt tctttaatta
     3661 aatagatata actcatacaa actcctctac aatttcaact tttctccatt atactccaaa
     3721 ataataaaaa aaactcctaa atactccatt tttatcttta cttttatgaa agcggatgct
     3781 atattgtttt tggagttgtt atccaaaaat aataaaaaat tggagtgttt aggataatgg
     3841 ataaaaagag gtttttagat cttatttcaa aaggtttgat tgtttcctgt caagcattac
     3901 ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc
     3961 aaagggctgg agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa
     4021 agtgtgtatc attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat
     4081 tcattactgc cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg
     4141 ctcttgattg cactcttaga aatcgacatg atggactatc tataaattct tttattacta
     4201 aaataaaaga aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag
     4261 gcaaaaatgc atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg
     4321 aggaaagtaa aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag
     4381 ggatttgtgt aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa
     4441 aaataggagt tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat
     4501 caagatttgt taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa
     4561 ttaggacgcg ctttcatgtt accaattgct atattaccag tagccggatt actattggga
     4621 ctgggaggag cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg
     4681 tggttgcata caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta
     4741 gcactaattt ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct
     4801 ggtttagctg gtggagtagc ttatctagta tatactgcaa ctattagtgg gtttcttgaa
     4861 cttttttcag caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt
     4921 ggtactgttg catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt
     4981 ttttttggag gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt
     5041 tctttctttt atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa
     5101 attgcccaaa tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt
     5161 gctgttggac tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc
     5221 gaaactgtag ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct
     5281 gatccaaacc acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca
     5341 acaatgatgt ttggattacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa
     5401 aatcgtaaaa agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga
     5461 attactgaac cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat
     5521 gcattcttag atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat
     5581 tccttttcag gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa
     5641 actaattggt taaaattaat tccttttggt attgcatggg catttatata tttctttgtt
     5701 tttacattct ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa
     5761 gttcattctg aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt
     5821 attgaagctc ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg
     5881 cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca
     5941 actgctgtct tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta
     6001 ttaagtcaag aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt
     6061 gaaattttca aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt
     6121 tccatcacag aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc
     6181 gtcatacctg atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt
     6241 cctactaaac atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt
     6301 ggaattgata cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat
     6361 gaggttactc ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt
     6421 aaagatactt ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata
     6481 gataatggtc gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta
     6541 tataaaaagc tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaa
//
LOCUS       DANJJW010000125         6570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000125 DANJJW010000000
VERSION     DANJJW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            127..579
                     /locus_tag="PWS82_002495"
     CDS             127..579
                     /locus_tag="PWS82_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907558.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="PWS82_002496"
     CDS             593..1282
                     /locus_tag="PWS82_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907559.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="PWS82_002497"
     CDS             1407..3758
                     /locus_tag="PWS82_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL0907560.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="PWS82_002498"
     CDS             3838..4233
                     /locus_tag="PWS82_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907561.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="PWS82_002499"
     CDS             4558..5175
                     /locus_tag="PWS82_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907562.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="PWS82_002500"
     CDS             5216..5515
                     /locus_tag="PWS82_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL0907563.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="PWS82_002501"
     CDS             5549..5716
                     /locus_tag="PWS82_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0907564.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="PWS82_002502"
     CDS             complement(5761..6366)
                     /locus_tag="PWS82_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907565.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DANJJW010000126         6413 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000126 DANJJW010000000
VERSION     DANJJW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..621)
                     /locus_tag="PWS82_002503"
     CDS             complement(40..621)
                     /locus_tag="PWS82_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907566.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(754..1437)
                     /gene="rpiA"
                     /locus_tag="PWS82_002504"
     CDS             complement(754..1437)
                     /gene="rpiA"
                     /locus_tag="PWS82_002504"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL0907567.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1756..2169
                     /gene="rpsL"
                     /locus_tag="PWS82_002505"
     CDS             1756..2169
                     /gene="rpsL"
                     /locus_tag="PWS82_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL0907568.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2253..2723
                     /gene="rpsG"
                     /locus_tag="PWS82_002506"
     CDS             2253..2723
                     /gene="rpsG"
                     /locus_tag="PWS82_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL0907569.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2817..4904
                     /gene="fusA"
                     /locus_tag="PWS82_002507"
     CDS             2817..4904
                     /gene="fusA"
                     /locus_tag="PWS82_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0907570.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5076..6263
                     /gene="tuf"
                     /locus_tag="PWS82_002508"
     CDS             5076..6263
                     /gene="tuf"
                     /locus_tag="PWS82_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0907571.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         1964 a         1267 c         1316 g         1866 t
ORIGIN      
        1 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
       61 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
      121 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
      181 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
      241 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
      301 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
      361 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
      421 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
      481 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
      541 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
      601 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
      661 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
      721 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
      781 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
      841 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
      901 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
      961 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
     1021 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
     1081 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
     1141 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
     1201 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
     1261 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
     1321 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
     1381 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
     1441 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
     1501 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
     1561 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
     1621 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
     1681 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
     1741 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
     1801 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
     1861 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
     1921 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
     1981 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
     2041 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
     2101 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
     2161 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
     2221 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
     2281 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
     2341 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
     2401 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
     2461 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
     2521 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
     2581 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
     2641 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
     2701 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
     2761 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
     2821 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
     2881 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
     2941 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
     3001 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
     3061 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
     3121 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
     3181 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
     3241 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
     3301 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
     3361 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
     3421 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
     3481 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
     3541 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
     3601 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
     3661 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
     3721 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
     3781 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
     3841 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
     3901 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
     3961 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
     4021 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
     4081 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
     4141 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
     4201 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
     4261 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
     4321 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
     4381 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
     4441 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
     4501 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
     4561 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
     4621 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
     4681 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
     4741 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
     4801 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
     4861 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
     4921 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
     4981 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
     5041 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
     5101 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
     5161 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
     5221 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
     5281 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
     5341 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
     5401 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
     5461 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
     5521 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
     5581 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
     5641 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
     5701 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
     5761 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
     5821 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
     5881 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
     5941 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
     6001 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
     6061 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
     6121 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
     6181 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
     6241 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
     6301 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
     6361 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aag
//
LOCUS       DANJJW010000127         6303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000127 DANJJW010000000
VERSION     DANJJW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            334..714
                     /locus_tag="PWS82_002509"
     CDS             334..714
                     /locus_tag="PWS82_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL0907572.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="PWS82_002510"
     CDS             696..1610
                     /locus_tag="PWS82_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0907573.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="PWS82_002511"
     CDS             1855..2544
                     /locus_tag="PWS82_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907574.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="PWS82_002512"
     CDS             complement(2665..2751)
                     /locus_tag="PWS82_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0907575.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="PWS82_002513"
     CDS             3427..4377
                     /locus_tag="PWS82_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL0907576.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="PWS82_002514"
     CDS             4398..4931
                     /locus_tag="PWS82_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0907577.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="PWS82_002515"
     CDS             4947..5117
                     /locus_tag="PWS82_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907578.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="PWS82_002516"
     CDS             5567..6100
                     /locus_tag="PWS82_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907579.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DANJJW010000128         6287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000128 DANJJW010000000
VERSION     DANJJW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..177
                     /locus_tag="PWS82_002517"
     CDS             <1..177
                     /locus_tag="PWS82_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907580.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="PWS82_002518"
     CDS             190..690
                     /locus_tag="PWS82_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0907581.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="PWS82_002519"
     CDS             787..2634
                     /locus_tag="PWS82_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL0907582.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="PWS82_002520"
     CDS             2637..3533
                     /locus_tag="PWS82_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0907583.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="PWS82_002521"
     CDS             3583..3972
                     /locus_tag="PWS82_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0907584.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="PWS82_002522"
     CDS             3959..>6287
                     /locus_tag="PWS82_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0907585.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DANJJW010000129         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000129 DANJJW010000000
VERSION     DANJJW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            725..811
                     /locus_tag="PWS82_002523"
     CDS             725..811
                     /locus_tag="PWS82_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0907586.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWS82_002524"
     CDS             complement(932..1621)
                     /locus_tag="PWS82_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907587.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWS82_002525"
     CDS             complement(1628..1906)
                     /locus_tag="PWS82_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907588.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWS82_002526"
     CDS             complement(1866..2780)
                     /locus_tag="PWS82_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0907589.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWS82_002527"
     CDS             complement(2762..3142)
                     /locus_tag="PWS82_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL0907590.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWS82_002528"
     CDS             complement(3592..3879)
                     /locus_tag="PWS82_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0907591.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWS82_002529"
     CDS             complement(3899..4123)
                     /locus_tag="PWS82_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL0907592.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWS82_002530"
     CDS             complement(4578..4748)
                     /locus_tag="PWS82_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907593.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWS82_002531"
     CDS             complement(4763..5296)
                     /locus_tag="PWS82_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0907594.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWS82_002532"
     CDS             complement(5317..6159)
                     /locus_tag="PWS82_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907595.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANJJW010000130         6138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000130 DANJJW010000000
VERSION     DANJJW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(350..835)
                     /locus_tag="PWS82_002533"
     CDS             complement(350..835)
                     /locus_tag="PWS82_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907596.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(974..1189)
                     /locus_tag="PWS82_002534"
     CDS             complement(974..1189)
                     /locus_tag="PWS82_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL0907597.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1413..2213
                     /locus_tag="PWS82_002535"
     CDS             1413..2213
                     /locus_tag="PWS82_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0907598.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2232..4100
                     /locus_tag="PWS82_002536"
     CDS             2232..4100
                     /locus_tag="PWS82_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0907599.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4093..4584
                     /locus_tag="PWS82_002537"
     CDS             4093..4584
                     /locus_tag="PWS82_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL0907600.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4572..5126
                     /gene="srlA"
                     /locus_tag="PWS82_002538"
     CDS             4572..5126
                     /gene="srlA"
                     /locus_tag="PWS82_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL0907601.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5144..>6138
                     /locus_tag="PWS82_002539"
     CDS             5144..>6138
                     /locus_tag="PWS82_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL0907602.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2173 a          973 c         1226 g         1766 t
ORIGIN      
        1 taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa taataagaag
       61 aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa aattgaatct
      121 agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa gtagatacca
      181 aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa tagctgtaac
      241 gaattattag gttaatgcaa atgcttattt atactccgta taactttctc agaaaaaatg
      301 ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat caaaatggtg
      361 ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa aaaccaacgc
      421 ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa taatctcgat
      481 caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa cggtgggcaa
      541 taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca gtgctttctt
      601 gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta tgttgcgaat
      661 ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta gttgaaccaa
      721 cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt ttatatccac
      781 aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta tgcatgatag
      841 ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa aagcagatgg
      901 tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata acttttttaa
      961 taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg aaaaatttca
     1021 agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa atagttgttg
     1081 aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac agccagttca
     1141 accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt agaattctcc
     1201 tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat ataacgctca
     1261 ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca ttagtacaca
     1321 ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct tttatactgg
     1381 atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat attgcaggaa
     1441 aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc gaaaatttgt
     1501 tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata cagcatgaaa
     1561 acctactatc tttaaaagtc gatgtgtctt ctaaaacgga cataaaagaa gggatatata
     1621 aagtaatgaa acattttaag acgatcgatg gtctagtcaa taatgcagga ataaatattc
     1681 cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca gaacaagttt
     1741 ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa
     1801 gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg
     1861 aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac agttttactc
     1921 gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga
     1981 tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct tatacgagag
     2041 gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc cctctaggaa
     2101 ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca gaaagatcaa
     2161 gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga taaaggatag
     2221 gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta ctagtcactc
     2281 acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa
     2341 aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa
     2401 acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc atgcaaggaa
     2461 aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat ttactaaata
     2521 ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa gtcggtgtca
     2581 gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat gattttgatg
     2641 taaagattac aggcacacca aataaaggac taagaataat aggagatgag ctagaattac
     2701 gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca gaacagtttc
     2761 tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt aaacaagaaa
     2821 tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt aactatctgt
     2881 tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa ttcaatgaac
     2941 tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa aaagatttta
     3001 tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa gctaactctg
     3061 atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat ctcattgatc
     3121 tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg ataaatcggg
     3181 taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa aagtatcctt
     3241 tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg caacataaga
     3301 tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt cgatatcaaa
     3361 aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa ggaacagcat
     3421 taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata acacatgttt
     3481 cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt acaacgattc
     3541 cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt tttgatgact
     3601 cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg tttcattcta
     3661 ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat
     3721 tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa aggatcttga
     3781 aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct cacacaacca
     3841 accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa ggtttagcag
     3901 aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg acggctgaag
     3961 ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa atgggaatag
     4021 atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc aaaagtaaag
     4081 gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc agcttatgtg
     4141 ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata tagcgaactg
     4201 cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc aggcacgatt
     4261 gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt
     4321 acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat ccattacatc
     4381 gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc tttagaaaat
     4441 gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca accttcattg
     4501 ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa agaaaaaagg
     4561 agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg ctgttccaaa
     4621 caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc ttgatgctga
     4681 tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg aaagtagcca
     4741 aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta tctgctttta
     4801 tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac tacaaaccaa
     4861 gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc ccccatatta
     4921 atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc ttagggctaa
     4981 attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac tttattggtg
     5041 gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc gttcttagca
     5101 aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg gtgatattaa
     5161 gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga caaaagataa
     5221 acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag ataaaataga
     5281 agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat
     5341 cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc ctaaaaaagg
     5401 gataccaacc atcaatatcg ttcccacagg aaaaagtggc ccattagcac aatatatcac
     5461 tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa tggaaactga
     5521 tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat atgatacttc
     5581 taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa ttggaatggg
     5641 cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag atacgatgat
     5701 ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga tacaaggttc
     5761 tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg cagttggatt
     5821 aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg gtccaggggc
     5881 agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag gaaatattcc
     5941 tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat gtgattttat
     6001 tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag gtgtaccatc
     6061 agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt gggtagcaag
     6121 ttttggatta tatagctg
//
LOCUS       DANJJW010000131         6104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000131 DANJJW010000000
VERSION     DANJJW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            231..1238
                     /locus_tag="PWS82_002540"
     CDS             231..1238
                     /locus_tag="PWS82_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0907603.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1250..2254
                     /gene="neuB"
                     /locus_tag="PWS82_002541"
     CDS             1250..2254
                     /gene="neuB"
                     /locus_tag="PWS82_002541"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL0907604.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2251..3399
                     /gene="neuC"
                     /locus_tag="PWS82_002542"
     CDS             2251..3399
                     /gene="neuC"
                     /locus_tag="PWS82_002542"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL0907605.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3372..4049
                     /locus_tag="PWS82_002543"
     CDS             3372..4049
                     /locus_tag="PWS82_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0907606.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4039..5181
                     /locus_tag="PWS82_002544"
     CDS             4039..5181
                     /locus_tag="PWS82_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0907607.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5194..>6104
                     /locus_tag="PWS82_002545"
     CDS             5194..>6104
                     /locus_tag="PWS82_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0907608.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKM"
BASE COUNT         2269 a          772 c         1164 g         1899 t
ORIGIN      
        1 ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca aaaaaaatat
       61 gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta atcaatcaaa
      121 tgttgattcg ttacgttttg acatcattat tgataattag tttagttata tattttttaa
      181 agattaagaa tcgaattata gatgactata attcatagga ggagatataa gtgtttaaag
      241 gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt gttcgtcagc
      301 ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa aaacaatttt
      361 atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt gatatcagag
      421 actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat gctgccgcaa
      481 tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg
      541 gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt gtaggtatca
      601 gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa
      661 aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt tcttgtgtaa
      721 ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt aaacaaataa
      781 aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg atgtcaatac
      841 cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga gaaacatatg
      901 tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata aaaatatata
      961 atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc aggccaggag
     1021 aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata
     1081 aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa
     1141 aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga caggaattta
     1201 ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga
     1261 agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga
     1321 aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac
     1381 gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga
     1441 ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag
     1501 aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga
     1561 aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt
     1621 aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac
     1681 aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt
     1741 tactatttta cattgtactt caaattatcc ggctagttct gaagatatta atttattggc
     1801 gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg
     1861 atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac
     1921 cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc
     1981 agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc
     2041 aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa
     2101 tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg
     2161 aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc
     2221 agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca
     2281 cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat
     2341 gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg
     2401 atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat
     2461 aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa
     2521 caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg
     2581 atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt
     2641 actgtagatg aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct
     2701 acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc
     2761 gtcggagctc ctagaataga tactatttta aataatagat tacctagttt tagagatata
     2821 aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt atttcatcca
     2881 gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa ttcaatggaa
     2941 caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag cgaaattatc
     3001 agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa tttagagccg
     3061 gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc ttcttctggg
     3121 ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag acaaaatgga
     3181 cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat tgaaactgct
     3241 ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa gaacatctac
     3301 ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt gaatgatggg
     3361 gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt gatccataca
     3421 ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga ttagaaaata
     3481 agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg aaatatcttg
     3541 ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat aaatttattg
     3601 tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat atgccaagtg
     3661 agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt tcaattggtt
     3721 atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg
     3781 ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac tatgttcatt
     3841 tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact caaataggaa
     3901 ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga
     3961 gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca gctaaaatta
     4021 agaaagaagg aattaatttt gaattctaga attttattat caagtcctca tatgggagga
     4081 acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc attaggtgaa
     4141 aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg cggagcttct
     4201 gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa tgttggaaaa
     4261 aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc tattttatat
     4321 caaggggctg taccagtgtt cattgactca gaacctaata catggaatat gagccctgtt
     4381 gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa ggcaatcatc
     4441 gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat tgcaaaccaa
     4501 tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta taagggaatt
     4561 tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa gatcatcaca
     4621 acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca ttcaagattt
     4681 ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat aggttataat
     4741 taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt attgaatgat
     4801 agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc tggaattgat
     4861 agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt gtctacttta
     4921 acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa actaaatgaa
     4981 aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt atttcaaggg
     5041 gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt taatagcggc
     5101 ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt tattgatgag
     5161 tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat tggtttaggt
     5221 tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat tttactatgt
     5281 ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg aataccaact
     5341 tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt tgtttgtaca
     5401 tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg taatatcttt
     5461 acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc taaaaagaaa
     5521 gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca atatattaag
     5581 tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca gtatttaccc
     5641 gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa aactaatggg
     5701 tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg gaaagttaaa
     5761 tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc ggacagtttc
     5821 actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga tgttgtttca
     5881 agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga atgggaaggt
     5941 aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga tattcaattg
     6001 tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga tgcatattta
     6061 gaggagatta aagaattttt cacgcttttg gaaaagaaaa tgga
//
LOCUS       DANJJW010000132         6001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000132 DANJJW010000000
VERSION     DANJJW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..395
                     /locus_tag="PWS82_002546"
     CDS             <1..395
                     /locus_tag="PWS82_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907609.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="PWS82_002547"
     CDS             398..1228
                     /locus_tag="PWS82_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL0907610.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="PWS82_002548"
     CDS             1244..2095
                     /locus_tag="PWS82_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0907611.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="PWS82_002549"
     CDS             2092..2451
                     /locus_tag="PWS82_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL0907612.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="PWS82_002550"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="PWS82_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="PWS82_002551"
     CDS             2624..2929
                     /locus_tag="PWS82_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907613.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="PWS82_002552"
     CDS             2929..3240
                     /locus_tag="PWS82_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL0907614.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="PWS82_002553"
     CDS             3237..3410
                     /locus_tag="PWS82_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907615.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="PWS82_002554"
     CDS             3407..3823
                     /locus_tag="PWS82_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL0907616.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="PWS82_002555"
     CDS             3823..4491
                     /locus_tag="PWS82_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL0907617.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="PWS82_002556"
     CDS             4488..4865
                     /locus_tag="PWS82_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907618.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="PWS82_002557"
     CDS             4862..5161
                     /locus_tag="PWS82_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907619.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="PWS82_002558"
     CDS             5151..5309
                     /locus_tag="PWS82_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907620.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="PWS82_002559"
     CDS             5403..5819
                     /locus_tag="PWS82_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL0907621.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DANJJW010000133         5961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000133 DANJJW010000000
VERSION     DANJJW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(451..1233)
                     /locus_tag="PWS82_002560"
     CDS             complement(451..1233)
                     /locus_tag="PWS82_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907622.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="PWS82_002561"
     CDS             complement(1298..2134)
                     /locus_tag="PWS82_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0907623.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="PWS82_002562"
     CDS             complement(2152..3561)
                     /locus_tag="PWS82_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0907624.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="PWS82_002563"
     CDS             complement(3575..5398)
                     /locus_tag="PWS82_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0907625.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1845 a         1283 c          966 g         1867 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa c
//
LOCUS       DANJJW010000134         5809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000134 DANJJW010000000
VERSION     DANJJW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            450..1604
                     /locus_tag="PWS82_002564"
     CDS             450..1604
                     /locus_tag="PWS82_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL0907626.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="PWS82_002565"
     CDS             1598..2020
                     /locus_tag="PWS82_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907627.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="PWS82_002566"
     CDS             3143..4075
                     /locus_tag="PWS82_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907628.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="PWS82_002567"
     CDS             4092..4607
                     /locus_tag="PWS82_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0907629.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="PWS82_002568"
     CDS             4903..5184
                     /locus_tag="PWS82_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907630.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="PWS82_002569"
     CDS             5186..5602
                     /locus_tag="PWS82_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907631.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DANJJW010000135         5790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000135 DANJJW010000000
VERSION     DANJJW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(99..1229)
                     /locus_tag="PWS82_002570"
     CDS             complement(99..1229)
                     /locus_tag="PWS82_002570"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL0907632.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PWS82_002571"
     CDS             complement(1877..3079)
                     /locus_tag="PWS82_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0907633.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PWS82_002572"
     CDS             complement(3139..3342)
                     /locus_tag="PWS82_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL0907634.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PWS82_002573"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PWS82_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PWS82_002574"
     CDS             4879..5691
                     /locus_tag="PWS82_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907635.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DANJJW010000136         5680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000136 DANJJW010000000
VERSION     DANJJW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(107..475)
                     /locus_tag="PWS82_002575"
     CDS             complement(107..475)
                     /locus_tag="PWS82_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL0907636.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            625..1107
                     /locus_tag="PWS82_002576"
     CDS             625..1107
                     /locus_tag="PWS82_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL0907637.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1144..2154
                     /locus_tag="PWS82_002577"
     CDS             1144..2154
                     /locus_tag="PWS82_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL0907638.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2269..2982)
                     /locus_tag="PWS82_002578"
     CDS             complement(2269..2982)
                     /locus_tag="PWS82_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907639.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3172..3762)
                     /locus_tag="PWS82_002579"
     CDS             complement(3172..3762)
                     /locus_tag="PWS82_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL0907640.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4246..4473
                     /locus_tag="PWS82_002580"
     CDS             4246..4473
                     /locus_tag="PWS82_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL0907641.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4489..5655
                     /locus_tag="PWS82_002581"
     CDS             4489..5655
                     /locus_tag="PWS82_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL0907642.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT         1914 a          922 c         1117 g         1727 t
ORIGIN      
        1 caattaagtg tttaaccagg gatgataaaa aggataggat tgaacaaaac ctgtataagg
       61 tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat aaagctttat cgccaaaatt
      121 tatcatgacc ttttttatct tcaatattaa atgaaatcaa ttgttcaatc aattcccagt
      181 tgattacact tttccaagga actttgatga cgttatccgt atatacgtag cctgcttctt
      241 ggatattttt ttcaagggca cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt
      301 tagcaaaact aaatgcaata ataaaggtac catggtcagt aaacataggt tggttccatt
      361 ttacagttgt gtctagctgg ggatacgttt ctgttatctt agcaaaaagg tctttgatct
      421 tttgttgatg ttctttttct ttgatttgat tgatgatcgg ctgaaatttt tccattctaa
      481 aacgcctcca ttaagtggtt tgatttattt taccagaaaa acaatagtta tgtataaaaa
      541 acgaacaaaa cacaagtaat gttgtaaccg ctatatttat atgataagat gtctcaaata
      601 ataaaataga aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat
      661 atatattttg agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata
      721 aagaaagaac aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa
      781 gataataact acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg
      841 aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga
      901 caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat
      961 gaagagctct tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg
     1021 gtcatccaaa cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg
     1081 atgccggcaa acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc
     1141 aacatgataa gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc
     1201 tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa
     1261 aaatctttcc aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt
     1321 ccagcaagaa aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg
     1381 atctttccga cttggggagg agaattattg attgagattc tcgattttgt cgattttgat
     1441 gctctacaac caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc
     1501 acattaaaaa cagggatagc cactgcacat ggaactaacc ttattgattt aagaggagag
     1561 tacttggatg aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg
     1621 attctacagc aatcttctga aaagtatcaa aaagtgtggc cccatgagca ttcatcatct
     1681 tctgttttcc atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg
     1741 atgaagggac gattattagg tggttgtatt gatacgatca ggcatttaat cggtactcct
     1801 tatggagatg tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt
     1861 ctagaaaact gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg
     1921 gcaggctggt ttgatggcag ctcggggatc atgttcggta gaagtgcggc agaggatttg
     1981 ttagattata aaatgaagga tgtatacgaa gatctgtcaa aggaattaaa actaccgatc
     2041 ttgtatgata ttgattgtgg tcatgtaccg ccacaactga cattgattaa tggtgcctat
     2101 gcagaagtag cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt
     2161 ttttcctgcc ttggagtcta tcaaagacca ataaatctaa atagaaaaga aagtgaaaga
     2221 agctgacaaa atgctgtcag cttctttttg tataacatat tagaaagtct aattatctat
     2281 aactgtcaca tcgattaact gtttcacgtc attgggattt cctgtaggaa tagctactac
     2341 agatgcatta tcaatgattt cttgagttgt ttctctaatg aacgctggat aagatgtttg
     2401 atctgcaggc atttgccaat gtgaaggaac tggataacta gtgattgtcc cgtcatggtt
     2461 acttgaatac actaaaactc cctggtaact gtatttccca gtgagcataa ccacatcttt
     2521 cggatagact gcactccctg catcataagg ggcgataggt gttcctgcag gataatgaat
     2581 aacatgtaat tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa tagctagcca
     2641 aacacgtgcg tactcaattt gtagatcaga atatcctgct aacggatcta ttgactgaga
     2701 agactgtgag gaggttgtgt ctattgttga agaagaaata tttgtctcaa ctgtactact
     2761 agatgtagaa gaagtattgg gctctgagac tttactgctt gttgaagatg tattcgtttc
     2821 agtagaagct gatttctgag tacttgattt ttcagctgaa ctggtttgtc tgattgaact
     2881 tttgtctgtg gtcgcagatg gatcactatt ttctttacta ggagacgtac atcctacaag
     2941 gaataaagag accaacaata aaataaaacc taactttttc atataaaata cctcattatt
     3001 tttttaatac cagtatagca taatcctgaa tttttgaatc cgtaaaacct tgtctatgaa
     3061 tataaataga gtgtatcttc tttgataaat gtcagaaaaa gtgtgacaaa taatcatcag
     3121 taaaattact tgtcacacta ttgataagga gtatttaatt gaagagaatg attaggagaa
     3181 aggatctccg tactttttat agtaatcata gaatccaagt ttgtatagac gaatcgtttt
     3241 ttctcttatt tcttctgttt caaaatggac tgcttgaaga atcaagtctg tgaattctat
     3301 aggagctgtt ccattcgcag taatcaaatt acgatctttc acagcttgtt gttctataaa
     3361 atcttcttta ttctgataat tttccgattc taaccaagga aattgcgcat ttccggtgtg
     3421 tttgtaacct tgcaataatc cgctctgagc taaataatca actgccccac aaatagcagc
     3481 tactggtacg tctgttttta acaatttatc gattatttga tataaatgat gattttttat
     3541 gtcccatgaa ttgccaccga tcataataaa aaggtcaact ttttcaggta tttgttcgat
     3601 taagtaatcg atttttgttt ttaatcctcc aatggaagtg acctctgatt ggatagaagc
     3661 agttttgttt tgccatttct cattttggtt tagtgtgcta gtaatataag caccttccca
     3721 atcggcatat tcctctaata gaaacgtaat cgcagttttc attttcaagc tctcctttac
     3781 tatctataac aaggacaaca gttttaacct acagacattc attaaaattg ggtagcgaca
     3841 gcgaaaacaa caattcttaa gttttagaca gagtatacag atagtcaact taattaaata
     3901 cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc agcattataa cattttgcat
     3961 acatggatga caaaagaacc aattcccact tgcttgagta caatattaaa agaaactgga
     4021 aggcaaaaag taattataga aacgaaaaat aaatcatatc gggcactcag acatgaaaac
     4081 aaccctagag atatataact aggtaaccag tttaatgaaa gaaaaaatca tccaagaagt
     4141 cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc gtatatgaga
     4201 ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa aatagctttt
     4261 catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac cgtttatcga
     4321 gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt agagctaggt
     4381 ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg cgattttaag
     4441 aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat gatagaaaca
     4501 ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg cgagcatatc
     4561 tgctattact gtgattttaa taaagttttt ttagaagggc agccagtaga tgagtatgtg
     4621 gaaatgctgc tgaatgaaat gaaaataatg ctagaaagtc atccgacagc gaatttagaa
     4681 acactttacg tagggggcgg gacaccaact tctttatctg ctaaacaact tgatcgactg
     4741 ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac tgtagaagcg
     4801 aatccaggtg atttgacccg tgaaaaactt caagtaatga aaaactatgg cgtcaatcgt
     4861 ttatccatgg gagtgcagac atttgataat cgcttattga aaaaaattgg acgtaaacac
     4921 accgcagaag atgtgtatca aactatgcaa tttttagaag cagaaaattt taccaatgta
     4981 agcatcgatt tgatttatgc attaccggga caaacactag aaggatatct ggatactttg
     5041 gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat cctcgaaaac
     5101 aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga tcaggaaacg
     5161 gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg tcaccaatat
     5221 gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat gtactggaac
     5281 aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga tattcgttac
     5341 cgcaacaaag gaccaatcca acactattta cgcccattac gagcaaatga acgcccggta
     5401 ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt cttaggacta
     5461 agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa aacaattgaa
     5521 tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt taatgaagaa
     5581 gaccgtgttt atttgacacc aagagggacc tttttgggga acaatgtgtt tgagcgcttt
     5641 ttgtttgaaa aatagtttaa agaatatttg ttttttatct
//
LOCUS       DANJJW010000137         5656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000137 DANJJW010000000
VERSION     DANJJW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(190..867)
                     /gene="nth"
                     /locus_tag="PWS82_002582"
     CDS             complement(190..867)
                     /gene="nth"
                     /locus_tag="PWS82_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL0907643.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(874..1572)
                     /locus_tag="PWS82_002583"
     CDS             complement(874..1572)
                     /locus_tag="PWS82_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0907644.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1840..3024
                     /locus_tag="PWS82_002584"
     CDS             1840..3024
                     /locus_tag="PWS82_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL0907645.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3030..3473)
                     /locus_tag="PWS82_002585"
     CDS             complement(3030..3473)
                     /locus_tag="PWS82_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0907646.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3699..3986)
                     /locus_tag="PWS82_002586"
     CDS             complement(3699..3986)
                     /locus_tag="PWS82_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907647.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4157..5509)
                     /locus_tag="PWS82_002587"
     CDS             complement(4157..5509)
                     /locus_tag="PWS82_002587"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL0907648.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1492 a         1136 c          957 g         2071 t
ORIGIN      
        1 tttctacatc gggtattttc ccaataaaaa taacaacttt tatttttatc gcaataaatt
       61 gtaataagtt accatcctag ctactgagta tgtacattta ctttttcatg caaaaaagat
      121 aaacgaaaca gcttttctcc ataataacaa gataattcat acatttattt gcaattaaag
      181 aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt
      241 tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt
      301 gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg
      361 tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct
      421 caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc
      481 atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc
      541 tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat
      601 gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat
      661 tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt
      721 attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg
      781 attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc
      841 ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga
      901 gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg
      961 ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca
     1021 cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg
     1081 cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg
     1141 ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga
     1201 ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc
     1261 acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat
     1321 cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg
     1381 atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc
     1441 aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct
     1501 atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc
     1561 taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag
     1621 caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac
     1681 ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta
     1741 agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt
     1801 tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa
     1861 cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc
     1921 agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc
     1981 tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc
     2041 ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca
     2101 agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt
     2161 atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc
     2221 acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga
     2281 acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata
     2341 tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa
     2401 agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac
     2461 caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct
     2521 gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt
     2581 cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc
     2641 tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga
     2701 atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta
     2761 ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact
     2821 cctgtattca atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg
     2881 ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag
     2941 ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc
     3001 tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat
     3061 cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta
     3121 gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg
     3181 attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg
     3241 atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta
     3301 gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct
     3361 agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata
     3421 atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca
     3481 ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt
     3541 ttgtaggaaa acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc
     3601 cctttttctg attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt
     3661 catcacttaa ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct
     3721 agcacatatg gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct
     3781 gagtagatgc gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct
     3841 ggcatcgtgt aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac
     3901 tcatcaatat tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct
     3961 tgttggacaa aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa
     4021 agctgcttgt tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg
     4081 aagaaagaca actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg
     4141 tcacaaccct ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta
     4201 ggttcaagta attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga
     4261 gatcttctac agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa
     4321 tgtcgtattt ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta
     4381 gctccatcat tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata
     4441 cagtcgcttg gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac
     4501 cgatcgtcca gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt
     4561 tatgatctgt caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag
     4621 gaatatagtt ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa
     4681 aaatgtctgg attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg
     4741 tatctacttt tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca
     4801 tatctgtatc aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg
     4861 tgataaattc agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt
     4921 tttctaagat atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat
     4981 caatcaatgt cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat
     5041 atccaccacc aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg
     5101 cttgattgta gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga
     5161 taataggcca tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc
     5221 ctgttttcag atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt
     5281 gttccatttt aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc
     5341 ctgctgggtc ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa
     5401 tattgtcatt tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt
     5461 ttacagctgc agtacctgca tgagtacagc caattactac tactttcatt tatggtccct
     5521 ccgattaaca ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag
     5581 tttaatgctt gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga
     5641 acccaatatc tacaag
//
LOCUS       DANJJW010000138         5636 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000138 DANJJW010000000
VERSION     DANJJW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            502..1059
                     /locus_tag="PWS82_002588"
                     /pseudo
     CDS             502..1059
                     /locus_tag="PWS82_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="PWS82_002589"
     CDS             1117..3156
                     /locus_tag="PWS82_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL0907649.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="PWS82_002590"
     CDS             complement(3265..4416)
                     /locus_tag="PWS82_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0907650.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="PWS82_002591"
     CDS             complement(4570..5100)
                     /locus_tag="PWS82_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0907651.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="PWS82_002592"
     CDS             complement(5199..5549)
                     /locus_tag="PWS82_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL0907652.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2044 a         1033 c         1050 g         1509 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaa
//
LOCUS       DANJJW010000139         5583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000139 DANJJW010000000
VERSION     DANJJW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(60..2009)
                     /locus_tag="PWS82_002593"
     CDS             complement(60..2009)
                     /locus_tag="PWS82_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0907653.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="PWS82_002594"
     CDS             complement(2051..3109)
                     /locus_tag="PWS82_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL0907654.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="PWS82_002595"
     CDS             complement(3228..3683)
                     /locus_tag="PWS82_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907655.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="PWS82_002596"
     CDS             complement(4267..5070)
                     /locus_tag="PWS82_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907656.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1647 a         1101 c          773 g         2062 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 gga
//
LOCUS       DANJJW010000140         5557 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000140 DANJJW010000000
VERSION     DANJJW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            759..1145
                     /locus_tag="PWS82_002597"
     CDS             759..1145
                     /locus_tag="PWS82_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0907657.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1239..1430
                     /locus_tag="PWS82_002598"
     CDS             1239..1430
                     /locus_tag="PWS82_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907658.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1944..2276
                     /locus_tag="PWS82_002599"
     CDS             1944..2276
                     /locus_tag="PWS82_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0907659.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2572..2727
                     /locus_tag="PWS82_002600"
     CDS             2572..2727
                     /locus_tag="PWS82_002600"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL0907660.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3003..4343
                     /locus_tag="PWS82_002601"
                     /pseudo
     CDS             3003..4343
                     /locus_tag="PWS82_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4584..>5557)
                     /locus_tag="PWS82_002602"
     CDS             complement(4584..>5557)
                     /locus_tag="PWS82_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0907661.1"
                     /translation="EVDLSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGI
                     SSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIES
                     LNYWVASLPKIECQMIIQKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCL
                     TDSLNADNFRKISPFPWKKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFV
                     FSIEKENETTEDAIKYAIVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRI
                     RVKEDNSPDQIAVRTGWISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT         1898 a         1028 c          901 g         1730 t
ORIGIN      
        1 accacgatag gtaactttaa acaaacatga agagacttta ataagacttc tttatctgaa
       61 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
      121 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
      181 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
      241 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
      301 ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc aatttgggaa
      361 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
      421 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
      481 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
      541 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      601 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      661 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      721 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      781 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      841 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
      901 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg
      961 ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc
     1021 ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta
     1081 caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg
     1141 tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa
     1201 agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt
     1261 tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct
     1321 ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     1381 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     1441 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaactat
     1501 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
     1561 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
     1621 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
     1681 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
     1741 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
     1801 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
     1861 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
     1921 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
     1981 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
     2041 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
     2101 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
     2161 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     2221 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     2281 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     2341 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
     2401 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
     2461 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
     2521 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     2581 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     2641 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     2701 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     2761 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     2821 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     2881 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     2941 acagacacct taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca
     3001 agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct
     3061 ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg
     3121 gcacattgac ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga
     3181 tcattaaata gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga
     3241 ccattctgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag
     3301 cagaagattc agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc
     3361 ttgagctatt aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc
     3421 cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc
     3481 tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca
     3541 tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc
     3601 aactcaatcc tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc
     3661 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
     3721 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
     3781 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
     3841 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
     3901 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
     3961 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
     4021 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
     4081 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
     4141 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
     4201 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
     4261 gaaaatattt taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac
     4321 ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc
     4381 accaacactc attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat
     4441 tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag
     4501 cgcttaacac acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa
     4561 aaacctcctc atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca
     4621 atactaataa ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat
     4681 ccagttctaa ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct
     4741 ttaatctcaa caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg
     4801 atcttatcca aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg
     4861 atagcatctt cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa
     4921 gaggtaataa tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt
     4981 aaaaatttca ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa
     5041 atttttcgga aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta
     5101 atacattgtt gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac
     5161 aacggatgcc aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt
     5221 ttaggtaaac tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt
     5281 tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta
     5341 atattgccag gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac
     5401 cgatttcgat agataaaact tgagatacca tattcttctg tttcaggaat atgattttga
     5461 tagccaagct ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa
     5521 agatttgtat ttagaatttt cgataagtct acttcac
//
LOCUS       DANJJW010000141         5550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000141 DANJJW010000000
VERSION     DANJJW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            86..457
                     /locus_tag="PWS82_002603"
     CDS             86..457
                     /locus_tag="PWS82_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0907662.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(741..1826)
                     /gene="xerS"
                     /locus_tag="PWS82_002604"
     CDS             complement(741..1826)
                     /gene="xerS"
                     /locus_tag="PWS82_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL0907663.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2160..2393
                     /locus_tag="PWS82_002605"
     CDS             2160..2393
                     /locus_tag="PWS82_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL0907664.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2390..2797
                     /locus_tag="PWS82_002606"
     CDS             2390..2797
                     /locus_tag="PWS82_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0907665.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(3934..4452)
                     /locus_tag="PWS82_002607"
     CDS             complement(3934..4452)
                     /locus_tag="PWS82_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL0907666.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1851 a          902 c          878 g         1919 t
ORIGIN      
        1 agaagaattt acaaaagtta tttcaacttt ttatattgat gatttgtacc aacattttgg
       61 ttttatttgt atgtggttac tttttatgac cgttatgcgt ttgggagaag ccactgcatt
      121 aacttggaaa gatattgatt ttgaaaataa aaacttgcgt gtgaataaaa cactctatta
      181 taagagagcc aaaacctatg agtttgtaga acccgaaacg gcttctagta ttcgtacaat
      241 cgaactagac gacaatacaa ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat
      301 aggaggcatg tcgtttgtac tttcttataa tggaacacct actcagaaat atacaatccg
      361 tcatattatg agaagacatg caacgatcgt tggtgttcat ataatccgca tacacggatt
      421 aaggcatagt catgcttctt tgcttatatc acaataaaaa gatgcacttc gaattaaaga
      481 aaggttaaaa catgctgatg tacaaacgac tttaggaact tatggtcatt tgtatctaaa
      541 ttcattgaga gagataacaa atgatcccac agattatatc caaactaatt tttctaaaat
      601 caaaccgaca cctatttacg ataataaaaa ataaatgata aacaaaagta ggatcacacc
      661 aaaagaaaca cactagaaaa actataatac aggcttttct agcgtgttgt ttcttatttc
      721 cgcctattta tacattatac tcataaagca tttaatgcat cgatcgtagc cgcattgtct
      781 atatgggtat ataaagttgt tgtattggta cttgaatgtc ccaattgttg ggcagttaat
      841 accaatgaat ttgtctgttg gtagagtcta gtagctaatg tatgacgcag tttgtgaggg
      901 ctgacttgaa ttctaaagcc ttttgaatat tttgctacca tccgttcgat tgcaataccg
      961 gatatacgct tggcttctcc ttggtaaatt gctagaaata aagctttttc gttttctgtt
     1021 actttatatc gttctttccg aatagctaga taattagcta aatattccat tgcgaatgct
     1081 gctatattga cagcatctct tttatttcct tttctagtta ctaccacatt attcttatct
     1141 aaagataggt catctaaatt aatatttata acctcggaga gtctcaatcc agatcctaaa
     1201 atcagtgcat tgatcgccag gtctctttct acatctcgtt gataatagat caaagctcgt
     1261 ttactcaaag tatttttata ttcattggca atataatcta gataattttg tgtatcctta
     1321 tctagaaata attttttctc aatggcattg gcacgtgcag ctaacgtagt tttatctgtg
     1381 atggttgcta cttgatccat aggattattg tctaagtatg gttttcctag aggattattg
     1441 gaacttttcg aaagatattt aaatagaacc tttaatgccg tgatatttct attgatagta
     1501 gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt ctcttttttt
     1561 acattttctt ttgtgcgacg tttcaaatga gatttgtaca agtttaggtc ataggcagtt
     1621 aactgtctta tcgaggtgat agaagtatca ggaacgatag actcactcag aagccattca
     1681 taaaagagct tataattttt tagatattct aaagtggttc gaggtgattg atctttatcc
     1741 tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat tgtatctatt
     1801 ttttgatata tttctgtatt tttcatgtca ttcatccttt caataagtag gggagaagag
     1861 tatctctata attggactat accaattata gaatgttttg tcaattggta tgctctaaaa
     1921 tttattaatt tgtttcaaaa aaagcatata aacaggtata cgtttaataa tttaaaataa
     1981 atggtataca taaataggga aggaaataga agggagcaat ctaaattttt tgaattttct
     2041 taattaatta tatagaaaga acaatctttc tatagagtta gacatgagag ggactatgct
     2101 tgatttaaaa tcaacaagcg tatatactgt atatacaaag aaaaaggagg gagaagacta
     2161 tggcaattaa agaaagaaaa tttagggaag tggggaactc agtagtatta actatttcaa
     2221 aggagtttct taaagaaaat gggttaaatc ccggagatac ggttttttta gatgaagaaa
     2281 aacttcgagc ggcaattaca aaaaaacaag ccttatctga tgaaattgat ttagcaatat
     2341 ctcaagcatt aactgaatat gatactactt ttagagagct tgtcgataaa tgagtattca
     2401 ttacctagaa gccaaagata ttataaaaat gaatgtaatc caaataaaaa aatactctcc
     2461 taatgaaccc cttggtgtaa aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg
     2521 agcctcagct tttggtgaag aggtttatcc ttcgatttat gaaaaagcag ctatcctact
     2581 tatacaattg ataaagaaac atccttttca caatgcaaat aaacgtactg ctttcttagc
     2641 tacttttgtc tttttgaaaa tcaatggaca gcttttagct attgatcaaa aagatgctgt
     2701 aaatttagcg gtatatattg ctacgtatga taaggatttc gatcaactaa aatatgaagt
     2761 gatcaacact ataaagacac atacgcagcc catttaagga gttactgtat gtgtctttat
     2821 agggttatta gctgctatag cgaaataatt gaagtaaatt acaaatagct ataattcaaa
     2881 tccaactata tagaaaacgt aaattttcta tatagttgga ttaagataac gactatttat
     2941 tttatcatat ttttactata ccctatactt ttacttggta tatttatgaa taatcgcaaa
     3001 tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag ttatttgttc
     3061 ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac gaaagaataa
     3121 aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag tgagttagtg
     3181 cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct tgcagaaatc
     3241 ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt gaacttctac
     3301 tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc aaatcccgta
     3361 tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg ccacataccc
     3421 tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta gcatattaac
     3481 aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa tttcaattct
     3541 tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca tgtgggttgt
     3601 tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa
     3661 aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat atggcaccat
     3721 aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat
     3781 ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc caataaaaac
     3841 cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata caagaggtct
     3901 cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct tccattttct
     3961 tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa atttatatac
     4021 ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag
     4081 atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac gattgagaat
     4141 ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact tatataactc
     4201 tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc catttctttc
     4261 atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta aattttcagc
     4321 aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa tagtttcaaa
     4381 ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt ctttattttt
     4441 atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat tcatctataa
     4501 tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat tgactgtcta
     4561 actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa tgttgatcgt
     4621 cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga ctggtttgca
     4681 tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact aataaaaact
     4741 accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc acaaaatcca
     4801 ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt acagcatcgc
     4861 tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga cgatgacacg
     4921 ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact cgaagaaaat
     4981 ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat taaaggtcaa
     5041 gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc acaatgcatt
     5101 tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt aatgaaacag
     5161 atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat
     5221 cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt gtttaaggtt
     5281 ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt taagttggtt
     5341 gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg acagtaccta
     5401 aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg ctcatttcaa
     5461 cttatatgtt ttagtgaact acccccactt agcttggata ggctacaatt aactagacag
     5521 aaaaattaag gtgtgtagac tagaagaaaa
//
LOCUS       DANJJW010000142         5532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000142 DANJJW010000000
VERSION     DANJJW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..985)
                     /locus_tag="PWS82_002608"
     CDS             complement(<1..985)
                     /locus_tag="PWS82_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL0907667.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="PWS82_002609"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="PWS82_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0907668.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="PWS82_002610"
     CDS             1810..2964
                     /locus_tag="PWS82_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0907669.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="PWS82_002611"
     CDS             3179..4147
                     /locus_tag="PWS82_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0907670.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="PWS82_002612"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="PWS82_002612"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0907671.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="PWS82_002613"
     CDS             5177..>5532
                     /locus_tag="PWS82_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL0907672.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DANJJW010000143         5499 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000143 DANJJW010000000
VERSION     DANJJW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(70..468)
                     /locus_tag="PWS82_002614"
     CDS             complement(70..468)
                     /locus_tag="PWS82_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0907673.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(465..701)
                     /locus_tag="PWS82_002615"
     CDS             complement(465..701)
                     /locus_tag="PWS82_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0907674.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(861..1145)
                     /locus_tag="PWS82_002616"
     CDS             complement(861..1145)
                     /locus_tag="PWS82_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907675.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(1316..3544)
                     /gene="topB"
                     /locus_tag="PWS82_002617"
     CDS             complement(1316..3544)
                     /gene="topB"
                     /locus_tag="PWS82_002617"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0907676.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(3718..4128)
                     /locus_tag="PWS82_002618"
     CDS             complement(3718..4128)
                     /locus_tag="PWS82_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0907677.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(4245..5408)
                     /locus_tag="PWS82_002619"
     CDS             complement(4245..5408)
                     /locus_tag="PWS82_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0907678.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
BASE COUNT         1472 a         1149 c          903 g         1975 t
ORIGIN      
        1 aacataaaaa aatcagcata ttccttaatg acatgctgat ctaattgaga aataaagttt
       61 ttaattcgtt cattttattg cggtttgtcg taaataactg gttatcttgg actttagtga
      121 atcgaaatcc aatgaactag tggtaatcat tatgataaaa tcaacagctt cttgacgaga
      181 aaaagcaact ggacaattat tcaattgaag aaacaacaac atggcaacaa gagcagttcg
      241 tttattgcca ttatgaaaag gatgtttttt agctaaatta attgctaaaa tcgccgcttt
      301 ttcatagatt tcaggataga gttcttgatt gaatactgct tgtgaaggct gtttgacggc
      361 catatctaat gcatttgcat cttttacacc gatcatttcg cctggcgagt gacgtaaaat
      421 aactttcgcg ttgattttga taatttcttt agtcgaaaga tacctcataa atcaaccaaa
      481 tcttttaacg cgtcttcata ttgattcatg atcgaatcaa ctaatgaatc aatatcaggt
      541 tcttcttgtg ctttgatcat tcgtacagaa ccatcagatt gaaaaacata actgattgcg
      601 tcacctgtct gcacacccat gaattctttt acttctggtg ggatagttgt tacatcgcta
      661 ttgccagatt ttctaatttt atactttttc atttttttca tatttcgcca ccctctatac
      721 cattctcatt ctatagtaag tacattatat ccaagtatat acttttaatc aatgtatata
      781 cttttatttt gctttttctt acgaaaaaat gacaaagaaa aaagtttgtg acatgacttt
      841 tgtcacaaac tctcgtattc ttaaccaaaa tattgatgaa atacttgtgt aataactggt
      901 agaaaataaa taatgatcat agccgctacc accaccaaca attgttttcg ctgtttcttt
      961 cgctttagca tgcttgtcgt tttatgaaaa aaataagtag cgcttttagc tcctgcagtc
     1021 aaagtcgata aaatcaacgt tggtgaatat gctgtttgat tgtgcgtcaa tgacaacaac
     1081 ttcgtcccgt aatcaggata ctttagaaat gcttggtaca cagccacaat cgtgattgct
     1141 cccataagat aatttaaaaa tgaatgcact tttttcaaat aaaaaaactc cttgctatct
     1201 atataaaatc agcctacccc aacgtttttg cttgattcac tgatattttt tagttttatt
     1261 ggtatttata ttctcacttt tcggaataaa tattccgcta tttcattgtt ttcatttact
     1321 ctacttcgtt ttcattattt tttcttgatg tttctttgtt tccatcaact gcttttgttc
     1381 cactgttttc gatccatgga aaagaaaaac cgattgtcat atcttctttt aatactaaaa
     1441 ctgcatcgaa tttccctttt ttaccagtaa aacctttgac aactttactt gtctttcctt
     1501 ttgaaagcaa atctctgaga ttcgttttag tcagcttctt tttagctact gttggccaca
     1561 atttgaagtc gcattccgga ttcaagcatg tagcgatttt aggataaagc atgactattc
     1621 cctttttaca ttttggacat cttcctaatt gatcgttttt cttctccatc gcttttatct
     1681 gacgtgtccc gctaatctca tctgaaaagt ctaccttgtc gatttgatta ggaacttctg
     1741 ccaataaatg taggatgaat tttttgatat tctctaaaaa aacagatggt gtcccctctt
     1801 gtttaccaat ttttctcaag tacccttccc agcgcgcagt catttctgcg ctcgtcagta
     1861 atggttcatt ggctactgcc ttacaaagag tgattccttt tggcgtaaca gttatttcgt
     1921 ttttattgac ttcgatgtaa tgcttctgct taagattttc aatgatattg gctcgtgtag
     1981 cttctgtccc tattccttct acttctttga gaatttcttg cgcttcatca ttatccagcg
     2041 tttttcccgc agttttcatt gctgtgatca acgttccttc ggtatagggt tttggaggtt
     2101 gtgtttcttt ctccatactt ttcagttcta cttctgcttc ttcattttct acgataggag
     2161 gaagagtctg agcttctttc ttttttgact tgatgcgtaa gatttcctgc caaccttgct
     2221 gtaatgggat ctttccgatg ctttttagct ttaattgctg tacgcccgtc attacggtcg
     2281 tctcttcgta gatataatct gcagcaaaca tagccacagt agtgcgcaaa acttgcaaat
     2341 agattgcttg ctgtaatttg gaaagcttcg caaagcgtgc aggagtcgga atcgtctttg
     2401 tcggaataat tgcatgatgt tcttgaactt ttttcgcatc gacatatcgt ttctttgctt
     2461 tgatctcagt tgtctgtact tcttctattc ctaaaaattt tttataattt tgcagatgtt
     2521 taaccagata ctgatgctca ttttctgtaa tgtaaaagga atccgttctt ggatagctta
     2581 gaaacttgct ttcgtacaat ccttgagcag catcaagtgt agctttggca cttgctttca
     2641 ttagctgatt catctttgtc tgcaaactag atagcgaaaa taaacgtgga ctatgcaacc
     2701 tcttttcctt tttcgtaact tcaagaatcc gtccgttttg cttacctaaa tgcgctccaa
     2761 gtttttctat ttccttcgtt aactcttctt gggttttaaa tgcttttttt ggtacgattt
     2821 ttccagaaaa ctgccctttg tttgtcgtaa ttaaaccttc tccttcaaag tacgtttctt
     2881 ttttgaagtg tttgatttct tcttgcagct gataaatcat atagagtgtc ggtgtttgta
     2941 cacgaccaag agaaaaactg ccatcaatcc cttttcgttg aagtagcaag ctatataatg
     3001 gacttccatt cattccaatc agccaatcag cgttctgcct tgcttgcgct tctttatatt
     3061 ttggaaaata gttctttcca ggttggagat tcttaaaacc ttcatagatt gcttcttttt
     3121 caagagaatt gatccataaa cgtaaatagt tcttttcctt agaaaatgcg tttgcttgtc
     3181 gaataatcga ccacgcgatg gcttcacctt ctcgatcacc atctgttgca ataatgatgg
     3241 tatcggctga ctgcaattct cgttttacga tcgaaaattg tgcacgtttg tccattggaa
     3301 cttcgtaacg gaaggtttct gggaaaatag gcaaattttc taaagaccat tttgcccaac
     3361 gctcttcata agctcctgga gcaaccatat ctaccaaatg tccaaaacca taagtgatgg
     3421 ttacttcacc agcaaacaat ggatcgctga tttcataata cccttcatgc cgcttttgct
     3481 gttggaaggt ttcggcatag gcttttgctt gtgaaggttt ttcagctagt atcaccgtct
     3541 tcatcttctg acctccgtta cttaattttt tgcggacttc tctattatgg aacatatttt
     3601 cgcatctggc aataaaaaaa agataccgta tagtcgaaat agacacaaaa acatcaagca
     3661 aacaaacgat gatccgtttg ctgcttgatg tttccagaaa atattcatct ttagctgtca
     3721 ctcttggtta atctgccagt tgatgccaaa tcgatcagtt accatcccat acaactgagc
     3781 ccacggaaca gcatccagtg gcatctcaat cttaccaccc tcagctaagc tttcaaatag
     3841 gcgcttcgtt tcttcttcat ttcttgtctg gatgactaaa gaaacattat ttcccacaac
     3901 taattcagga ccaattcctt ctggcgtatc tgcaaacata actaatgtat cataaatgtt
     3961 cagccgtgca ttcgtgatta gattttttgc ttcttctggc aagttcattt gttctattcc
     4021 aggcatttca ccgtatgtgg tgatacctga tgcttcagtc tgaaaaactt tttcataaaa
     4081 agcgatggct tcacgcgact catttttgaa gttcagatag atatccaatg taaacagctc
     4141 ctctcttggt taaatctacc ataagaaagc gttttttacg agtgaatcgt ttgattgatt
     4201 catgacaaaa gccttgcgca gcttcttctt gatctttttt atgatcattt attcaaaaaa
     4261 tctgtaatag cagattgatt catttgatta tcaatttcaa gaatactgcc agcataatca
     4321 ctttgcccat aattccacga accgtctact ggcacactta ttcgatcaat gccaccagct
     4381 cctcgtgcaa tcgaaaagag gtttttcgtc aagaaccaag cagtgacatc tgtggatgta
     4441 tatcctaaag ctttaccagc tgcataaggt gcacggatca gattcaatgg attcttcaat
     4501 tggctgaaaa tagcgttcat tacttgctgt tggcgtctga cacggccaaa gtctccttgt
     4561 tcatccattc ggaaacgtgc gtactgcaaa agaacatgtc catccatctt ctggacccct
     4621 tttttaatat ctacaccatc cagattcaga tctttttctg catcgatctt cactccgttc
     4681 atgaataagg catcgatcac tttttcaaac gatttgaaat cgactttcgc atagtattga
     4741 caatcgattc caaaattttc tactaaagtt tgacgcacta gatcggctcc tccgtatgca
     4801 taagctgaat tgatcttatt ttgtcctacc cctgggatcg taacgaagct gtcacgcata
     4861 aaagaaatca gtttcggctt atgggaagga ccatccaact gtaagaccat gatcgtatcg
     4921 gctcgcccgg catcttctcc gcgtgtatca cttccaagga tcaaaatatt atgcgcacca
     4981 ttcgtcgctt tttgcccatg aaaggtttct gaagtcgctg cctctaagct tgtgtcatgt
     5041 tcagctacat atttaccaat aaaaaatgag atacctgaat aaaggatcaa aacaagcaaa
     5101 atcgatagaa tgattttttt ccacgatcgc tttcttcgag gttttaacgg tttgttcttc
     5161 ttttgccgat tgaaaaattt tttatttttt tgttgttctt ctgctcttgg tgcatctctg
     5221 tatatctgtt gctcgtattg gttcaaatgt tcaaatgaat gctgctctct ttcataaata
     5281 cgggtagcat cttcctcatt ttccatacgc gttcgttttc gttctcttct agcaaaaata
     5341 ctccccgtat cccgcttcag aggtttcgcc ttcttatgga tgtccttata gcgatccatc
     5401 cgactcattg tggttcctcc tcgtttctta ctgataaata aaggatacga taaccaatcg
     5461 acaaaagcct atcatttttt gataatttaa taaaaaata
//
LOCUS       DANJJW010000144         5220 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000144 DANJJW010000000
VERSION     DANJJW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1256)
                     /locus_tag="PWS82_002620"
     CDS             complement(<1..1256)
                     /locus_tag="PWS82_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0907679.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="PWS82_002621"
     CDS             complement(1270..2487)
                     /locus_tag="PWS82_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0907680.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="PWS82_002622"
     CDS             complement(2477..4072)
                     /locus_tag="PWS82_002622"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL0907681.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="PWS82_002623"
     CDS             4197..4982
                     /locus_tag="PWS82_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDL0907682.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DANJJW010000145         5061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000145 DANJJW010000000
VERSION     DANJJW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(205..660)
                     /locus_tag="PWS82_002624"
     CDS             complement(205..660)
                     /locus_tag="PWS82_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907683.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="PWS82_002625"
     CDS             complement(1063..1236)
                     /locus_tag="PWS82_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907684.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="PWS82_002626"
     CDS             complement(1917..2456)
                     /locus_tag="PWS82_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0907685.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="PWS82_002627"
     CDS             complement(2472..3395)
                     /locus_tag="PWS82_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907686.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="PWS82_002628"
     CDS             complement(4523..4678)
                     /locus_tag="PWS82_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907687.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="PWS82_002629"
     CDS             complement(4697..>5061)
                     /locus_tag="PWS82_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0907688.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DANJJW010000146         5061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000146 DANJJW010000000
VERSION     DANJJW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(77..298)
                     /locus_tag="PWS82_002630"
     CDS             complement(77..298)
                     /locus_tag="PWS82_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907689.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(295..585)
                     /locus_tag="PWS82_002631"
     CDS             complement(295..585)
                     /locus_tag="PWS82_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907690.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(582..1766)
                     /locus_tag="PWS82_002632"
     CDS             complement(582..1766)
                     /locus_tag="PWS82_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0907691.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1948..3351)
                     /locus_tag="PWS82_002633"
     CDS             complement(1948..3351)
                     /locus_tag="PWS82_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0907692.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3373..4146)
                     /locus_tag="PWS82_002634"
     CDS             complement(3373..4146)
                     /locus_tag="PWS82_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907693.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4156..4533)
                     /locus_tag="PWS82_002635"
     CDS             complement(4156..4533)
                     /locus_tag="PWS82_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0907694.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4554..4868)
                     /locus_tag="PWS82_002636"
     CDS             complement(4554..4868)
                     /locus_tag="PWS82_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0907695.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1473 a         1018 c          878 g         1692 t
ORIGIN      
        1 tgatcatttg taaacatatt cataaatcct ttcataacaa aatatatact tcctatttta
       61 tcatgaaaaa tatctgttaa gcaccaatga ttcggttaaa taagtttaat aacacatctt
      121 tcacgccagc tgcattgaac acaagtccga cggcgattag agcaataatc aagaagccaa
      181 tgagttttga aaattctcgt ttaaaaccta aaaacagtcc aatgacaaca atcgctaaca
      241 ataccaatga ctgtgcatta tccaagaacc agttgtacaa attctgccca aaattcatgt
      301 gattgtcctc ctttccttaa gatatttttc tcgataatag atatagcttt ttacacgcct
      361 taataattga aaaggggtta aatgctctcc atagacggtt actcgattgg tataattatt
      421 ataaaatctc accttgtaaa aatcataggt atagatggta tcaccagctt gccgttttcc
      481 ctcaataatg agagacgtat atttccctgt attttgtata tacgcttgaa actcaatgcc
      541 acactgaaac cctaacttct ttaattcttt tgttaaaagg ctcatgtaat caaatcctcc
      601 atggctagat gttgttgttc aattaatttc tcatgttttt ctgtcagttt ggcttcctga
      661 atcatggtgg agataatggt tgtaccgttg agaacatcta aaatgcttgc gatttttaat
      721 gtaggggcga cttgatgatg taaccagttc aatgttcgtt caaaagaata aggctctggc
      781 tgtgtggtta ggcgtaatgc tccacgattt ttaccgataa accattccca acgttcatta
      841 gttttccaat cactccgacg tttagtgtcg tctttatctg caaagcggat atagcgattg
      901 ataatttcaa aggctgtttt ttccgcctgt tgatgtgcca ataaatcttt catggcttgg
      961 gtggctcggt cattttttaa ccgaatttca aaccgattct ttacttctgc gtcttctaag
     1021 ggaacatcat ttttcacgta ttgctcataa tctttctcat atatacagaa atagacttca
     1081 ctttttaatg agccaatata aagtgtgttt cccatgtatg gtttttcatc acggtgaacc
     1141 aattcaccac tgcgataatt tttaaagctt cgaaagacgg aaatgcattc ttcctgttta
     1201 catttctttg ccaaatctgg aatattcaaa atccctgttt tatcgttgat agctaaatcc
     1261 aagcgtttga aaataccttt atgttccatg caatcttgaa agaaatcata ccagctccga
     1321 tgttgagcca atagaaagtt ttcaaattgc cgacagcctt ttcctttcag ttctaataaa
     1381 acacctttac ttacatcatg agagactagg acaacaatat caccgaaacg atagtgttcg
     1441 ggataagaat aatagccaaa gtcttcgtgt agcatataat ccatattcaa acgtaaaacc
     1501 tcaccgataa tttgagccac atcattggta gggaatcgaa tccgtacata gtcgaataac
     1561 atctccaatg ggttatcggg attgaagcgt tccaaagcgt tcagtaaatc ttctttcacc
     1621 tgttgagtag ggactgcctt gcctgtttca atatccgata ggtaaggtct ggtaatatga
     1681 gaagcaaccg ccagtttatt ttgagaaacg ccataagcca gtcgcttttc tttcaattcc
     1741 ttaatccaaa ttttttcgtt ttccattcgc aaacctccaa tctaaaaaag atgtaaacta
     1801 tgacccttga gtttacacct ttcatatttt ccgcaatcct ttgtaccatc aacattggtt
     1861 gatgagaatt gcctgttttc agttgtattt gtgcccccct gttagataga gggggctaaa
     1921 ggattggcgt ggctaacgcc acaccaatca aagcaagtct gaacctgtac ctttcacttc
     1981 gtgcggtaca ggctcagatt gctttttttg aatgactttt cctatttctc ctaagaaatc
     2041 gtaccttttt ggtacaaggg gtgtataaaa ctcactaatc acactttttc ctgtatccac
     2101 gtaacctctt cccttgatgg gttttaagaa aaattgtttt tgtgtatcgc ttccgaacat
     2161 cataccaaag cccatttcag acatacgacc caaagccaca cggaagttaa agttgtcccg
     2221 aatcccatca ctaaaatatt ttgcgtctgg acgttgacag gcaagaatta aaaagaagcc
     2281 tgcttgtcgt cctaacatta caatttgttt gagtttatga ataacggtgg tactttcacg
     2341 acctagcata tccatcattg ccacatattc atcaaaaata agaaaattgg cagacaaacc
     2401 aagataagca taattttctc ctgttttata gttagggtgt tgtttcatgg tttcactgcg
     2461 atcaatcata tcttcgtaaa attgattgat acatgccaac atatcttctt tacggtaata
     2521 gacattattc atgaccgccc ctaagtctgc caaatcagca tttttagggt caagaatata
     2581 aagctttgca tttgtatgca ggagagattc aatcaatgtg agaataaaat aggattttcc
     2641 actaccagtg cccccagcaa ttaacatgtg agggagagaa tcgaactccc aatagaccga
     2701 ttccatcaat ttgacacgtc catcttgagc taccacgtct tcaatgccaa ttcgattgcc
     2761 aatagtatca taaagtagga catactctac ataagaatct ttcaacgttt tttccactaa
     2821 ttcacaatac aatcctgttt ctagtttgtt ttctaggtgc aaaagctgat cttgatattt
     2881 ccctaaggtg atttctacat gaatatggat tagtccatct ttcattcgat aatatatttt
     2941 aggaaaatgg cggattcgtt gtttggtttt cttgctgggt aaatctttaa agaatccctc
     3001 tgattgagtt tcttcggctt cataccaatg attttctaaa atcatacgag ctaacttttg
     3061 acggtgctct aactgtttta tagaatctat acgatagtga taataaagcc agctgataag
     3121 tccgcataca aaaagagcag tgagaatact gctccaaaag tatggcgtat gaagttgata
     3181 gaaaaatgta tctaactgaa ctgcttgcca gttcaccgat tgtaacgctc tccaatgaaa
     3241 agggagtagg acgactagaa aaactaccag caatgaaatt agtacaaaat gatagaccaa
     3301 gtgcttatcg ttggctttga ttcgctctcc tctgtcatga agcaatggca taaaaatcct
     3361 cctttccacc tatcaaaata attgtgtatt gtagatacga gcctgtttat caaaagttgg
     3421 ttgagaaatg gcttcatatt tctgataagc gattcgcttt tcaaagcgta aatttttttc
     3481 tttcggataa taatgacgtc gatagagagt gtgtcctaac agttttttcg cttcttttgt
     3541 caacggatag agatagcgaa acatgcgtcc attgattttc tcaatccctt tgtattcgca
     3601 aaatgcctgg gaaagccaat gctttttctc tacctgttca aagcgagcat tttcctctaa
     3661 taaaaggcga gcacttcgag ggtgtatctt ttcccatgac tgcttatcac gatagacact
     3721 tgtcttgaaa tacccgcagt aaaagaagtt ggacgcttga tagacatagc cacattttcc
     3781 ctcaattcca tctgccagtg tatataaaaa atgacagtcc gtatgttctt tcaaccactt
     3841 aattagagct gaaagagctt gactgccaaa ataattggtt tgattcattt ctggaagaaa
     3901 acacatcttt ccaatttcta aataatctgt ggtttgaagg gaactgtcgg gaaagagttt
     3961 gcgtatggtt tgtaggggtt gcgtacccca gcctaattcc acaacgccta ataatttttc
     4021 ttctgaaaaa atccctagaa agtatttaca caatctaggg agtatctttg aataatggta
     4081 ttgccgaata aaagaaatgg cttcatcctt cggaatttct ttcaaaatca atggtgtgtt
     4141 tgtcataagc aacacctatt tttcactttc ggacgcttct tttttatatt tctgatgagg
     4201 ttttgtctgt ccttttaata caagatcgtc tgctttcacg taccaatcaa cgtctgcccc
     4261 tcgaaaagta gccgtcgcta cggtatctgc cacaggattg attaattcca cttctgcgtt
     4321 gtagtcaaat tctttcaaag gaacagaagc ggggatactg acttgtatca tgcgtccttg
     4381 tccttttgat tttaagtcat aggtacgttc tttaatttct tcggaaaccg taccatcttc
     4441 attctggata cgaacctcac gacgcaaggc ggaaaatttc aatgtgccaa atgtctggtc
     4501 tttttcaatc acaatacctt ctgctaatct catatttttt cacctttcct ttcttatgct
     4561 ttcaccatat cgtctgcgtg cataatgtaa ttggtaaatc ctcgattgcc aattttataa
     4621 ccttctgcgg taatatgtgg gttgatgagt ttgacttttt cgtcggattc aaaatgtttc
     4681 tcgccagctt cggagggaag aatgacaaca atatcatctg ctcgttgaat cgttgagtat
     4741 aaattgtaac tacgagagag aatggttggt ctgccattta ttcgacgttg ttcaatgttc
     4801 ccttcaccag catattctaa ttgtccaaat gttttttcca tattgggaat gacatattta
     4861 agttccatat ttttacctat cctttctgtt tttatgtttg aatgaatctt atttgtgtga
     4921 tggttttaaa ggggggagag cgaccttttg attcttgatt ttttattttc ataagttcac
     4981 ttcctttcaa aattgggtaa aaaaatagac gctccgtact gagcgtccga ttcctactat
     5041 atttaaaaca acgactacct a
//
LOCUS       DANJJW010000147         4972 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000147 DANJJW010000000
VERSION     DANJJW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            23..646
                     /locus_tag="PWS82_002637"
     CDS             23..646
                     /locus_tag="PWS82_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907696.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PWS82_002638"
     CDS             1096..2145
                     /locus_tag="PWS82_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0907697.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PWS82_002639"
     CDS             2142..3023
                     /locus_tag="PWS82_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907698.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PWS82_002640"
     CDS             3026..3796
                     /locus_tag="PWS82_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0907699.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PWS82_002641"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PWS82_002641"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL0907700.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT         1670 a          857 c         1074 g         1371 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gt
//
LOCUS       DANJJW010000148         4954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000148 DANJJW010000000
VERSION     DANJJW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            2..718
                     /locus_tag="PWS82_002642"
     CDS             2..718
                     /locus_tag="PWS82_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0907701.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            737..2320
                     /locus_tag="PWS82_002643"
     CDS             737..2320
                     /locus_tag="PWS82_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL0907702.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2455..3033
                     /locus_tag="PWS82_002644"
     CDS             2455..3033
                     /locus_tag="PWS82_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907703.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            2979..3497
                     /locus_tag="PWS82_002645"
     CDS             2979..3497
                     /locus_tag="PWS82_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907704.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3505..4545
                     /locus_tag="PWS82_002646"
     CDS             3505..4545
                     /locus_tag="PWS82_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0907705.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            4794..>4954
                     /locus_tag="PWS82_002647"
     CDS             4794..>4954
                     /locus_tag="PWS82_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0907706.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1829 a          568 c          970 g         1587 t
ORIGIN      
        1 aatggtcgat aattatgaaa ggaaaaatat tcttaaaaaa aagagtatag gatgtcgtat
       61 gaaaatagtc gctatagtac caatgaagtt aaataacaaa agattgccac aaaaaaacac
      121 gaaagctttt accaatggaa aaccattgtg ttattatata ctttcaacac ttttaaaagt
      181 aaaacagatt gataaagtgt atgtctattg tagtaaccca gatattaaag aatttatacc
      241 agaaggtgta acatttttaa agagatctag ttccttagat caggatacaa cgagtattac
      301 acaggtatta gccggtttca caaaagaagt agaggctgat gtatatgtaa tgacacacac
      361 aacagcacca tttattagcg aaaaatctat tgaaaaagga ctagatgccg tgctgacagg
      421 agaatatgat tctgcatttg ctgcaaaaaa attgcaagat ttcttatgga aagacggtga
      481 accatttaat tatgaattgg acaatattcc tagaacacag gatttgccgc ctttatacga
      541 agaaactagt ggattttata tttataatcg caatgtgatg accgagttgc atagaagaat
      601 tgggaaaaaa ccttgcatag ttgaagtagg tgagattgag tcagttgata ttgatgaagc
      661 cgaggatttt atgattgctg atgcgattta taatcatctt attttacaag gtagttaaga
      721 cagggggaag gttaggatga acaatgtaca gattttagat tgcacactta gagatggggg
      781 ctattgtaac aattgggaat ttggatttga taatacaaaa aaaattattt ctggacttat
      841 agaatcagga attgagatta tagaatgtgg gtttctcaca aataaaacag tatacaataa
      901 aaatgttaca aaatatacga ccttagaaga attagcacag ataattcctg aaaaaagaga
      961 aggaaaatta tttgtagcaa tgatgaacta tggcgaatat aatatagagg atcttccaga
     1021 ttatgatggt tcatctattg atggaattag gattgcgttt cataaaaaaa attgcaatga
     1081 agcgttgaaa ttatgtcgtg agataaagaa aaaaggctac ttagtttttg ttcaagctat
     1141 ggtatctctc aattatacag atgaagagtt tttagcagta ataaaaaatg tgaatcagtt
     1201 taaaccatac gctttttata ttgttgatag ttttggaatg atgaaaggaa aagatttaac
     1261 tcgtcttttt tacatgattg agcataatct agaaaaagat atacagatag gttttcactc
     1321 acataataac atgcagcttg catattctaa tgcacaaaaa cttataaccg tacaaacaaa
     1381 tcgaagcctg attattgatt cttcggtgta cggaatggga agaggagcag gtaatcttaa
     1441 cacagaacta tttgtagaat atttgaatga taatgcagga aaaaaatatc aattaaaacc
     1501 attattatat ataattgatg aaatactaaa tagtttctat cagaagagtt attggggata
     1561 ttcattacca aactatattt cagcagttca taatgctcat cctaattatg caagctatct
     1621 gtcatataag aatactttaa caattgaagc aatgaatgaa atattcgaaa tgatggatga
     1681 agaaaaaaag gtatcttatg ataaacaata tatagaagat ttatactaca aatatatgga
     1741 aactgggaaa atacaggagg aatatatgga agaactgaga aaatttctct cagacaaaaa
     1801 aattctactg attgcaccag gaaaaagttc tgtagatgag gtagaaagaa taaaagagtt
     1861 tgttaccaaa gaagaagtcg taataattag tgtaaatttt gaatattctc atatagatat
     1921 agattacgta tttttaagta atttaagacg ttttcatgaa ttaccgaaag aaagcagaac
     1981 taaatgtatt gttacatcaa atatacaagc atcggatgta tatttacgga cgagttataa
     2041 aaagttgtta tctgaaaggg aagttgtaag agacaatgca ggattaatgg caattagatt
     2101 tttagccgat atgaatgtgg atgaaatatt cttagctgga tttgatggat attcacataa
     2161 tgaagatgag aattatggtg agcaatccat ggaaattata acaaaatttg cattattaga
     2221 tgcaatgaac gtgcaaatga gagatgaatt gtcagaatta gctaaaaaag taaaggtgac
     2281 atttttaacg gatccacgtt gggttcgaat tgataagtag tagtaataat gtttttaaaa
     2341 ttttgataaa gagatagtga gagaataaaa atgggtaaag tcttaaataa aaaagaagaa
     2401 aaatatttaa gggacgatgg ggcgagatga aactctaatt ttgaactttt aagaatcatt
     2461 tctatattgt gtattgttgc ttaccatgct aatttgcgtt taaattataa tgcagacgga
     2521 attatagtac atggttcatt aactgctaac cagattttct tgaattatat tggtatttgg
     2581 ggtcagctag gagttaattg ttttatattt atttcggcat attttttaag tgagaaaaga
     2641 aacattcact ttgaaaatgt tttgggaatt gtattagaat gtatttttta tagtgtatta
     2701 gtttatggaa ttctttgtgc atgtggaata gaaacatttt cattgaacag ctttgtaaaa
     2761 gaaatattat catttgctct aggtagatat tggtttatta catcctattt agcattgtat
     2821 attctaagtc cgtatttaaa tatcatgctt aacaatctgg aaaaacttca gttgcaaaaa
     2881 ataagtatgt tattgtttat aggtgtatgc tgttatcgta tgttatttaa aacatctttg
     2941 tttggggatt gtgctgtttt aattgcacta tatgtcatat gttatacact gaaaaaaacg
     3001 ataataatat ttttgagaga cacagaatca taggaacaat tgtatgtata acagttatac
     3061 cacttctcat atccatagga atacttttca aatttatacc aaatgcaatg gttaaccaga
     3121 tggttcatat tggttctcca ttgatggtta tagcaagtat atttttattt tatgtattta
     3181 aaaatttttc attttacagt aagtatataa ataaacttgc gagaaccagt ttaggggtat
     3241 atattttaca tgaaaatcat tatttcattg atggtatgaa gtctagaggc ttaatatggg
     3301 atgggatatt tggattaggc aaaaggtttt tagataggga tttttggatt tggtttataa
     3361 ttgcagttgt agttgtgtgc ataatttgca tggctatcga tgttgtaaga gaaaaatttg
     3421 aaagacagat ttttaaaatt aagagtgtaa agaacctgtg taaacttatg aatcatgtta
     3481 taaatatata cccataatta ataaatgagg agaaaagaag tgaaagttat atcattcgga
     3541 gaattattat tgagacttgc agcacccggt tattcaaagt tatttcaaag agatagtttg
     3601 gagactacat tctgcggagg ggaagctaat gtagcggtat ccttagcaat atttggatat
     3661 caatcagaat ttataacaaa gcttccggat tcagatgttg gatatgcggc agctaatgct
     3721 ttgcgttatt ttggagtaaa tgttgataat attatatttg gagagggacg catgggactg
     3781 tattatttag aaaaaggagc agctcagaga ccatctaaag taatttatga cagaaaattt
     3841 tctgcaattt cgttagctga tagtgatgaa tttaagtgga aggagatcct aaacgatgca
     3901 aattggtttc attggacagg gataaatcca gcattgtctg agaatctctt tgagacgtcc
     3961 ttagatgcat gtcaggctgc caaaaaaaga ggtataacaa tttcttgtga tttaaattat
     4021 cgatccaagc tttggacatc agaggaagca aaagaaaaga tggaacagat actaccattg
     4081 gttgatattt gtatttgtaa tgaagaagat gcagaaaaag tgctaggaat taaagctgag
     4141 catacaaata ttaatagtgg agaattaaat aaagatggct atgtgcaggt ggctagggag
     4201 attcacaaga agtttggatg taggtatgta gctacaactc tgcgtagaag ttattcggca
     4261 agtagaaatg gttggagtgc aatgttatac gaagcagaaa aagataaagc gtatttttcc
     4321 acagaataca acatacaaat tgttgatcgc gttggtggtg gagacagctt ttctgctggt
     4381 ataatatatg cgttatgtga aaatatgtcc ggacaaaagg ctattgaatt tgcgactgca
     4441 tctagttgtt taaaacatac tatagaaggt gattttaatc gaactacaat tgaggatgta
     4501 tataatttaa tgaatagcgg tggaaatggt cgcgtgttgc gttagtcaac atataaagat
     4561 aaatcacagt aatttttatg gtgattatct gtcttagtag tgattagaat aacgtgaaaa
     4621 taaattgttg agcgtttata ctgttttgaa aaataaattt aacatattga aaaatacaaa
     4681 tatatacgta tattataaag cgccgattgt aaaattaagc tagacaacta aaacagtcat
     4741 ttgtgctaca ctcaaaatag ttccgaccaa agaattataa ggagtgagtg caaataacct
     4801 atacctatct tacaacggat gaacttgtaa taatagagtc ttatttgaaa attaaccaat
     4861 ctgttgctaa aactgcgcat tgcttgaatc gttcaagtca aacgatccat aaagtatatc
     4921 tgttcttcaa gcaaagaaaa tcagccttag aata
//
LOCUS       DANJJW010000149         4861 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000149 DANJJW010000000
VERSION     DANJJW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..257)
                     /locus_tag="PWS82_002648"
     CDS             complement(<1..257)
                     /locus_tag="PWS82_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907707.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="PWS82_002649"
     CDS             complement(273..923)
                     /locus_tag="PWS82_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907708.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="PWS82_002650"
     CDS             complement(934..2106)
                     /locus_tag="PWS82_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0907709.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="PWS82_002651"
     CDS             complement(2125..2997)
                     /locus_tag="PWS82_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0907710.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="PWS82_002652"
     CDS             complement(3019..3939)
                     /locus_tag="PWS82_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907711.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="PWS82_002653"
     CDS             complement(3939..4208)
                     /locus_tag="PWS82_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907712.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="PWS82_002654"
     CDS             complement(4205..4585)
                     /locus_tag="PWS82_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL0907713.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1282 a         1060 c          795 g         1724 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 t
//
LOCUS       DANJJW010000150         4794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000150 DANJJW010000000
VERSION     DANJJW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(207..1169)
                     /locus_tag="PWS82_002655"
     CDS             complement(207..1169)
                     /locus_tag="PWS82_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0907714.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PWS82_002656"
     CDS             complement(1171..2610)
                     /locus_tag="PWS82_002656"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL0907715.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PWS82_002657"
     CDS             2795..4741
                     /locus_tag="PWS82_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL0907716.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1529 a          821 c          789 g         1655 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atag
//
LOCUS       DANJJW010000151         4788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000151 DANJJW010000000
VERSION     DANJJW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            564..989
                     /locus_tag="PWS82_002658"
     CDS             564..989
                     /locus_tag="PWS82_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907717.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="PWS82_002659"
     CDS             1019..1183
                     /locus_tag="PWS82_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907718.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="PWS82_002660"
     CDS             2141..3118
                     /locus_tag="PWS82_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907719.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="PWS82_002661"
     CDS             3128..3625
                     /locus_tag="PWS82_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907720.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="PWS82_002662"
     CDS             4001..4282
                     /locus_tag="PWS82_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907721.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="PWS82_002663"
     CDS             4284..4700
                     /locus_tag="PWS82_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907722.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa atcagcgcct ggatcccttg
      361 gtacaggcgt tacttggtag tatacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DANJJW010000152         4748 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000152 DANJJW010000000
VERSION     DANJJW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            72..1118
                     /locus_tag="PWS82_002664"
     CDS             72..1118
                     /locus_tag="PWS82_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0907723.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="PWS82_002665"
     CDS             1135..2358
                     /locus_tag="PWS82_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0907724.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="PWS82_002666"
     CDS             2447..3334
                     /locus_tag="PWS82_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0907725.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="PWS82_002667"
     CDS             3420..3650
                     /locus_tag="PWS82_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL0907726.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="PWS82_002668"
     CDS             complement(3707..4672)
                     /locus_tag="PWS82_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0907727.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1605 a          789 c          966 g         1388 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctat
//
LOCUS       DANJJW010000153         4720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000153 DANJJW010000000
VERSION     DANJJW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1097)
                     /locus_tag="PWS82_002669"
     CDS             complement(<1..1097)
                     /locus_tag="PWS82_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0907728.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="PWS82_002670"
     CDS             complement(1227..2603)
                     /locus_tag="PWS82_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907729.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="PWS82_002671"
     CDS             complement(2603..3259)
                     /locus_tag="PWS82_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907730.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="PWS82_002672"
     CDS             complement(3269..4546)
                     /locus_tag="PWS82_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0907731.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="PWS82_002673"
     CDS             complement(4565..>4720)
                     /locus_tag="PWS82_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL0907732.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DANJJW010000154         4710 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000154 DANJJW010000000
VERSION     DANJJW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            106..357
                     /locus_tag="PWS82_002674"
                     /pseudo
     CDS             106..357
                     /locus_tag="PWS82_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="PWS82_002675"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="PWS82_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0907733.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="PWS82_002676"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="PWS82_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="PWS82_002677"
     CDS             1786..1869
                     /locus_tag="PWS82_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL0907734.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWS82_002678"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWS82_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0907735.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="PWS82_002679"
     CDS             complement(2994..3857)
                     /locus_tag="PWS82_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL0907736.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="PWS82_002680"
     CDS             complement(3859..4131)
                     /locus_tag="PWS82_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0907737.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="PWS82_002681"
     CDS             complement(4148..4357)
                     /locus_tag="PWS82_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL0907738.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="PWS82_002682"
     CDS             complement(4455..>4710)
                     /locus_tag="PWS82_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDL0907739.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DANJJW010000155         4703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000155 DANJJW010000000
VERSION     DANJJW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            552..1463
                     /locus_tag="PWS82_002683"
     CDS             552..1463
                     /locus_tag="PWS82_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDL0907740.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1950..2216
                     /locus_tag="PWS82_002684"
     CDS             1950..2216
                     /locus_tag="PWS82_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907741.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2288..2629
                     /locus_tag="PWS82_002685"
     CDS             2288..2629
                     /locus_tag="PWS82_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907742.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2684..3103
                     /gene="ssb"
                     /locus_tag="PWS82_002686"
     CDS             2684..3103
                     /gene="ssb"
                     /locus_tag="PWS82_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0907743.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3180..3476
                     /locus_tag="PWS82_002687"
     CDS             3180..3476
                     /locus_tag="PWS82_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907744.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3491..3922
                     /locus_tag="PWS82_002688"
     CDS             3491..3922
                     /locus_tag="PWS82_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0907745.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4104..4409
                     /locus_tag="PWS82_002689"
     CDS             4104..4409
                     /locus_tag="PWS82_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907746.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1745 a          726 c          987 g         1245 t
ORIGIN      
        1 cgccacttat gagagaaaat ggtcaaaaac ctaagcggga agtctccgaa taacatacca
       61 agcgccgaca gccaacagat aaagaataca ctactaaaaa tagtagtagc ataaaattta
      121 aaagggaaaa aatcaacccc taccccagtc ttggggtctc aaaactaact cccccctaac
      181 cccctctttc agcctctttt gaaagagggg gagaatcaac cccacggcca acgccccagt
      241 gttcttgggg ctgtccagaa cagaaacttg cgagagagtg ggggtaaaac aagtgtagag
      301 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ccggagtaaa acaagtgtag
      361 aggaaacttg aagacttgtt cgaacagaaa cttgcgagag agttggagta aaacaagtgt
      421 agaggaaact tgaagacttg ttcgaacaca aacttacaag agaggggagt aaagcagttg
      481 cagaggaatc aggaagattt gttcgaacag aaacttgcaa gaaagcatta ttaaattgaa
      541 ggtgatgaaa tatgaagtat ttattgcatg gctattttat tgattattct actcgcaata
      601 gtagtacttt agcgattcta tgtttgcttc atgaattgag agtggttact agaaaacagc
      661 tatacaaact attgaatttt gacagatcaa ttagtaaaca aggagtaacg aatgctttag
      721 tgagattata tgaaaaacag ttaatcgatc gaaaaaaata tggaacagaa atgtattatt
      781 atttgacgaa agagggacat caaagcatag gtgggtatta tacgttgccg aaagttcctg
      841 aatataattt gaatcaccat ttacaaatta acaattattt gattaaagga atggcattgg
      901 caaaagacca cccacattta aaacggatta tttcagaaag acgtaccgtc tatgaaacga
      961 aggatttaaa tcctaagaca aaaggaagaa aattttttgt gccagatttt atttttcgtt
     1021 ttcgagacaa agaaaatcat gagatcaatt ggtcctttga aattgagcta acgttgaaaa
     1081 cacgtcgtcg ctaccgtgaa gcgatttttc cgaaatatat tcgtgagttg cgaacacgtc
     1141 ctgaatcaca tttaatttat gtgacaccta gtggcgcaat tcaagaagaa cttcatgcgt
     1201 ttaagcgtat ttttttcaag cgcgaaactg aaaaagatgc gagtcaacaa gaattgtttg
     1261 agcggttgca tattttttca gctttagagt ttgaagaggg attaaaagca ttattagaga
     1321 atgatccagc agtcaattgg cggacagtag aacgagaaaa aatttatccg aatggcgctg
     1381 ttcaaagtcg tttgaattta tttgaagaat caactgaaaa tgaggaaaaa ccaaaagtga
     1441 agtttacgcc tgaagtattg taaaagaggg tgttcgcttc gctctttttt tgtgccagtt
     1501 tgctggcacc gttcagacgc aagcgtctga accggctcgt ttttaggaag gactaacttg
     1561 tttgatttat ccgttgtcaa agaagtggac gttcgagagt ccctaatcgt atagcagtca
     1621 ttcggcgagt ataacctaaa acataacctg ccaattgcca agggtaaaat gcacgctgcg
     1681 cgcccttggc aatcgtcaga acatgttttc gagccggtta tgccgaacga ccgctatacg
     1741 aaaagggcct ctaaacagaa gtccactttg atccttgaca actgaaaccc aaaatcaaac
     1801 aagcaggcct tcgtctaaaa acgagccgaa tgaagacctt gggggttgca cccccaaacc
     1861 cccgtgcccc cgacacacta ccgaaaaggt gggggtgggt ggcgaaaaaa tcaaataaaa
     1921 cattctattc aagaaaggaa gaaacaacca tgacaactaa aaacgagcaa ctgaaagaac
     1981 aggcaagaaa agagttgcaa gaaagaggtt tgattattga ggggagcttt gaaggagatt
     2041 ttgaaaccta tattggttgt tatgcaagac cgataaacaa acctacagcc ttagacccaa
     2101 tgaacgaaca agaagtaaaa gaacaagaaa aacatgcagt gaatggtttt ccgcaagatt
     2161 tcactgaatg gtatgagtgg gaaattaaca acgggaagct tgaaaacttc ttataaaagt
     2221 cagatgacgt gcaaagagaa tgattctctt tgtacggtaa aaatataaag aaagaaggaa
     2281 aaagaaaatg gcaaaaaaca caccttatca agtaggagac attttttata gtagttgggg
     2341 ctacgaacaa acgaacgtga ctttttggca aattgtgaag ttaacagaaa aaacagcatg
     2401 gtttcagcct attcaaaaga aaacggtcga gcattattcg tccatgagtg gaaaagttgt
     2461 tccaatgaaa gacaagttta tagagtatcc tttagcaaaa actaaagaca gtattgtaaa
     2521 aaaacgaatt gatccagaaa cctatcctga tatgttatgt ggttatcgta gttgggacac
     2581 gtttcataga tataaaaatc aagctgttta tgaaagtagt tattattaag gggatagttt
     2641 cccctttcaa aaataataaa tagaagaatg gagagaaaaa atcatgatga attcagtgaa
     2701 tttagtagga cggttaacaa aagaagtgga tttaaaatac acaccaaaag gcaatgcgac
     2761 aggcacattt attttagctg ttaatcgaaa ttatacgaat gcaaacggcg agcgtgaagc
     2821 agattatatc cgctgtgtca tttggcggaa agctgcggaa actttagcaa aatatactgg
     2881 aaaaggaagt ttgattggca ttaatggacg tcttcaaacg aggagctatc agaatcagca
     2941 aggacagacc atgtatgtta cggaagttct agtgacagat ttctatttgt tagagagtaa
     3001 agaaatcaat gagcaacggt caaaatcaat ggcgaataat caaaataatt cttcaattcc
     3061 aggaaatgag attcaaattt cagatagtta tttgcccttt taagtgaaaa ataattttgt
     3121 tctatacctg atttcttcag gtatagaaca aaaaataaat agagatgagg cgatttttta
     3181 tgaaaatcat tgacagttac gatcaacgaa ccatgcaata tttgggctcg tttgattata
     3241 cagatgaaaa tattattaat tatgttgcaa gtttacctcc ttatcaaagc gttcgcttgg
     3301 tagattttgc aagtgatgaa ttggtattaa caacgatggg caacttttta gattatgtcg
     3361 gagacaaaaa atggttggaa aaaattttgt ctaaattgat tgcgaaacaa acaggagaag
     3421 aacccataaa tgcgattcaa ttgttcaatc gatatgaaca agaaaacaat gattaaagag
     3481 gagagaaaaa atgaaaagat taaaacgaag tatttttatt gtgctagcat tgattgattt
     3541 tgttgggaca ggttgtgaag caattgacgt tcaaaaagct cccttatcgg aaataaaaca
     3601 agcaataaca aaacaagatg aagaacaacc gctaaataca caagtagaaa tagtgaatca
     3661 aaataaaccg agtttttctg aagaagattt ttcgttagaa aatggaacca ggcaattttt
     3721 ttcagatttg gatcgtttaa atcgggtagg tgtagcaagt gcacttttgc ataaaagcag
     3781 gatgccaaca gaagaacgag accgattata cattaagcca actggctgga aacaaaagaa
     3841 aatgaagaat ggcgattatc tttataatcg ttgtcatttg attggctatc aacgtgcgac
     3901 aagtttcccc ataagtatgt aatacgaaac gggaggatta atcttaagat taatcacaac
     3961 taacctattt gacagttgga atgaaagcta tcatgtctgc tgaatcaact ggtctgatac
     4021 tcctacatgc aataaagtct actaggaagt tattgtatga agctaggtaa aggcggcaga
     4081 acaatttcta agtgtggaat catatgaagt tggatatgcc gggacgctat aaattactca
     4141 gggtgagact gtttttaatt aaactgacga acttgcgaat gtacgggtct ataaccgact
     4201 tatattcgaa agatataaga ttgcgaatgc aaagaacatt atcgaaaagt aagaatgttg
     4261 gtcatgaaat tcgagatgga agactagcgg tgtatctgtc aagagttggt aaaacatgtt
     4321 tgattgattc agatgagagt gggaatgcga caaaacgttt tacagaagaa atagagcaac
     4381 tagcagaact gaatatacga tatttttaaa tactttagcg gagaataaaa aaatcctcta
     4441 cagaaaacta cggagggcaa catgtgctat tgtttcaaca atacattttt aatttttacc
     4501 ataacaaaaa agtacttgaa aaaaaacaga aatatgttaa attaaaaatg aaaaaatgga
     4561 agcggttact tattcttttg aatctaagat aaaaattcaa aagaataaca taacccaaat
     4621 atgattatac ttgagattct aaatatttat cgaaaaggaa tttgttttaa ggaaaaatga
     4681 ttctatggac ggttattgat tta
//
LOCUS       DANJJW010000156         4543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000156 DANJJW010000000
VERSION     DANJJW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(109..1752)
                     /locus_tag="PWS82_002690"
     CDS             complement(109..1752)
                     /locus_tag="PWS82_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0907747.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1749..2108)
                     /locus_tag="PWS82_002691"
     CDS             complement(1749..2108)
                     /locus_tag="PWS82_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907748.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2269..2529)
                     /locus_tag="PWS82_002692"
     CDS             complement(2269..2529)
                     /locus_tag="PWS82_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907749.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2520..2831)
                     /locus_tag="PWS82_002693"
     CDS             complement(2520..2831)
                     /locus_tag="PWS82_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0907750.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2832..3404)
                     /locus_tag="PWS82_002694"
     CDS             complement(2832..3404)
                     /locus_tag="PWS82_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907751.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3737..3997)
                     /locus_tag="PWS82_002695"
     CDS             complement(3737..3997)
                     /locus_tag="PWS82_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907752.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3987..4175)
                     /locus_tag="PWS82_002696"
     CDS             complement(3987..4175)
                     /locus_tag="PWS82_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907753.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1342 a          967 c          641 g         1593 t
ORIGIN      
        1 ggtaaaaata ctgtcaaaac acaaattgct attattataa atattgtaac aaatagaaaa
       61 tatctctttt tcacttctat aactacccct ttcaataagg agtatacctt aaaattcaat
      121 ctcatccaac atatcaaatg cttcctcata atccatatca gttatttctt ctcgtttata
      181 taaagctgcc aaaaaagcat gaaacccatc tgttttacgc cttactggct cttttttcaa
      241 aaatgtttta ttccctaatt tgtcttcttt gataaacgaa ttatttgtat accaacgcat
      301 cataggatca tcaccaaaaa taaaccgttc atttgcaaaa ccatcttcaa taataggagc
      361 aacttttgac tgcacacctc ttatatttct gataaactca aaattatatg attctttctc
      421 tagaagaggt ttcagcaaat ccatacgaaa tccgtcggca caaacgattt ctatttgatg
      481 gatttttctt ttttcattta accaatcaat taaatgacga ggatcaattg atggctcatc
      541 aacgattgtc actaaacctt ttttctccca atccttaata ggcgctttca gtttgaatgt
      601 atctagaaac tttttcctag caaaactatg ttgctgccag ataaatttat catcaatttt
      661 aaaaagcaaa cccactgttg caaaatcacg aatacttgca taatcaaatc cagccacaca
      721 agaacgacca ttcaaatttg gcaattcttg tttagtagcc attaattttt catgagtcgt
      781 aatatctgat tctgcatcac cttctgtaaa attcattcgt ttaacaacaa attcacgcct
      841 acctgatggc tcatcttcta gttttttata ttctttcatc actgtagaaa acagacgttt
      901 tgaatatgct gaatcttctt cgaacattgg attagccttt gaccacattt taggattatc
      961 catttcatca atgttatcca gtttacaaat aaagggaaac aagcgatcat tcttgttctc
     1021 tccactaaaa atcttttgtg ctcgttccaa cattttgtca taaaaacctt ctctaacatg
     1081 accatttgta ccattataaa aagttcgagg atgttttatt ttccctagcc cactacgctg
     1141 aatatcaact aggtctgaag attcaaactg atggatttca tcaaattcta agcagccatc
     1201 tcttgcacta tccatagtct taggattgtt tgtcctataa ctaaaaatcg aattagttac
     1261 attgttgact atagccatct tagtcaaata aaattgtttt tccagtttgc gcatttgtac
     1321 catttcataa acttctttaa aagatacctt tccctgcttc tcagaattcg ctgtaatcgt
     1381 tacatcataa tttctgacac cgtagagagg gctaataaaa aatgagtctc ttgctgacat
     1441 gaacccattt ttcccgccgc ctcgcgcaat actatttaga atttcatcaa aaaacacctc
     1501 gtcgtcctct ttttgatagc agaaaatgaa aggtgtgata aatttttgat atttagctaa
     1561 aggaaaaaaa tttttttcag tgtacttgat aaacttttga atcatttctt catcaaaata
     1621 gatatcatcc cgaactagca cttcgttttt taaataagta attaactgaa ttctttcttt
     1681 attaaaaggg attacccctt tttcgtataa atctacgtat tcatcaaaat agaatggctt
     1741 taatgttgtc acagcaaatc actaccatct agcgctgcat tttttgtagg ttcgcgcttt
     1801 tcttctggca aatagtcagt aagttgctta attacacgtt gatatgcttg gtctctcgaa
     1861 ttatactgtt tagcaactgg tcgttcccgc tcgtaaggtt tttgattttc tgactgagag
     1921 aaaagctcat aatcaccttt ttcggaaata tcaatccaca tttcatcaag aagaattttt
     1981 aagcgtgccg cttgcgtaat caaaccagaa acgaccttct ttttatcctt atcaatatcc
     2041 ttaaacaatt tatttagacg cctttcttca gcagaaacaa gcctatttct ctcttgcaga
     2101 tcagccatca actcacttcc tttctacgtg gggagggggt cgtgtgaaaa tagcatcatt
     2161 tatttgcaga gttgacccca tccaccggtt cccaaaaatt ttatttcttc tcaaaatctt
     2221 ttgaccgggg gtatttcact ctatatatct tcgctttata ctgatttatc agccgtcttg
     2281 ctcgttgctt tctacaatat gtgcaaatgg actcaaggta taatcaatgt ctacattaat
     2341 attaaatgtc cctgttactt cattagcgtt acctattagt ctattgttga tagatatatc
     2401 ctctacagtt gttgttcctt caatgagctg actcttaaat agaaccaact tatcaatagc
     2461 cttatcaacc atagcgattg cttctgctcg tttgacatca tctctgtttg ctgtttttct
     2521 taccaccatt catcatccca ctttcttttc ttttgttggc cacgatattt catacggttg
     2581 tgccttttgt tatggcaatc tttgcataag gttcttaggt tgtttatatc tagagctaaa
     2641 tccggatgat actccagctc ctttatatga tccacttcaa gaattgaatc atgttgagtt
     2701 gttagtctac cctctgcttt acaccattgg cactcataat gatctcgctc taagcattga
     2761 cttctaagtt tcctccactc agttgatcca tagaacctag ctcttgcttg tttacttgaa
     2821 acatcaatca tttacatatc tctcttgtgc ccaatgattt ttctatctaa gtacttgtta
     2881 ttatgtgaat cataatattc aatagtgatg tcgttagctc cttttggtac ttggcattca
     2941 tattcttccg tatcccaatg ataagtaata tcgacaagac ctatcggcat ctcatccatt
     3001 cgttgacctt tataataaac ttctggtact gagtcagtat ctcttagttt gatttctaga
     3061 aggttcgtat tacttccatc atctaacgtc aattcagcaa tcctcttttt gatctgttga
     3121 attacttttc ttcctgtctc acctgtcatt ggtactaccg tctcatatcc agtaaactcg
     3181 caagtaaagc gttcaatagc ataagcgttt tgaagtggct tagcactaag aacagctgga
     3241 ttattgctat aaaaacgatc tactaataac ttccctatag gaatggcatc taaagctcta
     3301 tcagtaaaca ctaaagcatt gggataatct tcttgaattt tgtaagctag attgggtgca
     3361 gttacaacgt tatacccttc tctgatataa tcttttaatt tcatagtttt cctcctaaaa
     3421 acatctaata attacttgct aacaacttga tgtaatcata gttatcattc atttgatgta
     3481 cctctcaatg ttgtattgga tatactcgtc tttccaatag ccatgaccgc aatatatcag
     3541 cttgcattta tccacttcgt ttggtgtagc ttctcttagc atttcgacaa tagagtactt
     3601 ccctttgatt tgtacagaac gcacaacacg cactgaacaa tcaccaatgg ttcgaggata
     3661 ttcattagtt agtgatacat accagtagtt tctcattatg cagcctcctt tatgtaaaga
     3721 aaaagcctag cacctgctag actttgatac attcatttga ttttttgaga taagtatcta
     3781 catagatttc acctttgtcc ccgttcaacg taatttcaaa atacggtgct ccttttaagc
     3841 tagcgctgac taatgctttg ctattttgca gtgttttgca tgaccagact atgaaaatac
     3901 taaactcagg cactgctcca cttaggtcag caatctcatt ttcaattctt tcacgtacaa
     3961 tagccttgca tttttcgata aacttctcat gattcatttt gtacctccgc aagcgcataa
     4021 ccagctgttg atgtattgtt aaacgtagct gaacgagtaa ctcccgtagc cttcccagta
     4081 taaataaaac taaatcctgt tgttgttggc ttaaaattct ccacttcata aaaatgatat
     4141 gtttgtccgt tattagtaaa tacaatcaat tccatttttc aaaatcctct cttttttaaa
     4201 ataaaaagac cactcaaaga gtgatctaat atgtaatgca caggcaggga cgtttccgat
     4261 cctgtgcttg agtcatttga cgattcattt gtaccgaatc ccaaaactca atctaaccca
     4321 accaattatg taatagcaac ctacaagatg cacaaaacgc gtacgaaatt gcgcacccct
     4381 atatttttaa accgccgatg cctcggttac caaagtcact ggcaaggaat cgaaccttgc
     4441 agtttgcacc taaattttag atgagcgata cacctttcca ccacagtgac ataatgacaa
     4501 tagacagcaa ctgatgatag ataataagaa caatttagaa gga
//
LOCUS       DANJJW010000157         4480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000157 DANJJW010000000
VERSION     DANJJW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..933)
                     /locus_tag="PWS82_002697"
     CDS             complement(<1..933)
                     /locus_tag="PWS82_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HDL0907754.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELST"
     gene            complement(930..1388)
                     /locus_tag="PWS82_002698"
     CDS             complement(930..1388)
                     /locus_tag="PWS82_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0907755.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1385..2848)
                     /locus_tag="PWS82_002699"
     CDS             complement(1385..2848)
                     /locus_tag="PWS82_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0907756.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2830..3762)
                     /gene="pfkB"
                     /locus_tag="PWS82_002700"
     CDS             complement(2830..3762)
                     /gene="pfkB"
                     /locus_tag="PWS82_002700"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0907757.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3904..>4480
                     /locus_tag="PWS82_002701"
     CDS             3904..>4480
                     /locus_tag="PWS82_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0907758.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1341 a         1011 c          718 g         1410 t
ORIGIN      
        1 tgtacttaat tctttaatat tttgttttcc ttcttctaac atccaactac tcgctttttc
       61 ttcgccttcg gtaacaaaag gtgctactcc cttcgcccat gttgctcgcc cacataagac
      121 accattgaat gtcgaacctg attgttttgc aaaacgcaat gtatcttgaa atagtgcagc
      181 gctgactccc gcacttaaaa aaataaacgg ttgttcagtc actttcgatt gttcatagaa
      241 atacgcggat gcctctgctt gactataaca agtatcttgc ccatatcctt ccacaaatct
      301 catattcact ggtacttcta ctttcaatac atccacttga taacgttctt ttgaaaattc
      361 tcgcatggct tcaatcactt tatgcggttt tacttttgca tagtcaagag aagacggatc
      421 gccaatagtt gcatcgtaag aaaggagctc taaaaagaaa ggtatgcctt ctgcttcgca
      481 ttctgaacca atccgttcta taactatttg tttttgcaca ttaatctcta ctggttcatc
      541 aacgtcatag tacaataaaa atttacaagc atccgcaccc acttctttta accttttcac
      601 tgaccaacta ttcagtaaat ctggtagtcg gcctggcatt ttcgcatcat agccggtttt
      661 ttcataagct agcaacaaac cacattcttt ctctcgaagc gctgctgcag gcagaccata
      721 ttctggatcg agtaaaatag cggaagcata cggagtcagg tactgagaaa ccatctgttt
      781 aaattggatg atatcttctg atgtagcttc tttatattgg ttgatcattt tcttcaaagc
      841 accacgttgg tcgatagcta gcgcgctaat aattccagct tcatccgtca atcgcttcaa
      901 acgttcctgt ttttctatac tcattttctt catatatctt cacctatttc tttagttaat
      961 attgtagcta tttctatttt tgattttgca gctctcaact gttctgtaac ctcttttcgc
     1021 atcaatgatc gtgcaacgag agacaataat ttcaaatgag tcgttcctgc ttctgaatct
     1081 gggataaata acgcaaaaat aaaatcaact gactgtctat ctaaactgtt ccattcaatt
     1141 ccttttttca gtgtgacgat aatcaaacta ggttcattga ttgtttgaga cttcgcatga
     1201 gggatagcaa aacctgacat tattcctgta gtaccttctt tttctctttc cagaagtttt
     1261 tgatatactt ctttttcgtc atgagcgatt cctaaacaga atgtttgctt tgctaaaaac
     1321 ttaaatgctt cttcttgatt atctaatggc tgttgtaaaa aaatgttctc ttcatgaatg
     1381 attgtcatga taaacctcct gctcttcctt cttcgataac tgtacgattt gttgctttat
     1441 tatccctctt cttgtcttta tcagttactc ttttcttttg gatcaaacct agtatcactc
     1501 caccaacgat cgaaccaata agaatcgctg taacccattg gatttttccc gtcactacag
     1561 gtaacactaa aaaaccgcca tgtggggctg gtacttggac accaaacaga taggtcaaga
     1621 cagcagcaat tgatgagcct aacatcatcg ttggtaatac tcggatcggg tctttcgcag
     1681 caaacggaat agctccttcc gtaatatgag ttgatcctaa aataaaatta accaaacctg
     1741 cattacgatc attagtatca aaatatttac caaacaataa agtagcaaaa ccagtaatca
     1801 acggtggtgc aatgcaagct gctgacgcac cagccataaa agtcgtgttt ccttgtgtca
     1861 aaagagctgt tcctgttaca tatgccgcct tattaaccgg tcctcccata tcaaacgcac
     1921 acatacagcc aacaatcacg ccaagaacaa gtggattcga atattcaaat ccagctaaaa
     1981 agctcatcat tccttcattt atagccttca ttggacctgc cagcaaagac atcacgatgc
     2041 caataaatgc tacaccaata actggatata agacgattgc cttcaagcca tctagttgtt
     2101 taggtaaaat tatcagtagc ttttgcaaaa ataagataaa ataacctgct aagaacccag
     2161 ctaaaatacc tcctaaaaat ccagtaccgc cattcatggc tacgattcca ccaacaaatc
     2221 caacgactaa ccctggacgt ttcgcgatcc cttctgcaat ataagctgct aaaactggaa
     2281 ccatcattcc cattgctgca tcgtcaactg cttttaccat tgccgctgta gcattgtatt
     2341 gtgcatgatc tggatcagaa gaatagattc cccaaacggc aaatgaaata gcaatcaata
     2401 ctccgccagc taccacgaaa ggcaacatat gagagacacc gttcatcaaa tttttataga
     2461 tgctacgacc aaaattagca ccacttatcg cttctggttc taaagtatca gcagcttcat
     2521 tatctgctaa gacagtacct tttcctgcca atgcttcgtc aatcagccgt tgtgcatcct
     2581 tgattcctgt actgacagaa acatcaatga ctcgttttcc cacaaatctt tctgaatgga
     2641 catctttgtc tgcggcaata atgacagcat ctgcctgttt gatttcttca gccgttaatt
     2701 cattctcgat cccaatttgt ccatgggtct ccactttgat cgtgatgcct tttttcctag
     2761 ctgcttgctc taatgcctct tgcgccatgt aggtgtgcgc aattcctgtc ggacaaccgg
     2821 ttgctgcaat tagttgatat tttcccatct ttcgaacctc tcttccttga ttttgatttg
     2881 tttacttaat tcctccacat ccaaaaaatc agtcagtcct ttacgaaaag cagtagagct
     2941 gcctgctgct atgcttttac gtaacgtttc atcaagcgat cgtcgattca tgtatcctgc
     3001 taaaaaagtg cctaacatgg catcacctgc acatgctgta ttgacaactt ttccttttgg
     3061 cgagtttcct cgaatcaccc tgtctttcgt aaaaagaatc gctcctttct caccgaggga
     3121 taacaaaaca ttttctgcgc ctgactctac tagtcgttgt ccataaacaa agtaatcttc
     3181 atctgtaacc atttttcgtc cgaaccaaga agaaagttct tcctcatttg gtttcagcaa
     3241 aaacggtcga tagggtaaac aatccatgac ttcttgatca ctactatcaa taatcaaaca
     3301 aatccgactg atttctatta aaacctttgg agacagccct cttggtaaac tcccagaaac
     3361 acaaagataa tcccctgctt gtaatgctct tatttgattt aaaaaattat gaacagctgt
     3421 ctgcggtatt tcaggtcctt gattcactaa tttgtattct tctcccgttt gattgacttg
     3481 tgtaaataca ttgatacgcg tcataccagg cacttcaata aactctgttt ggatttgttt
     3541 ctgctgtaaa tagtcttcta tatactttcc tgtgaaccca gcagaaaaac ctaatgccgt
     3601 acttgggaaa tctagcatct ttaatactag tgacacattc accccttttc cgtttgcctg
     3661 aatgtcatcc tcttttgtgc ggtttaccat aaaaggatgc atctcatctg tttcaataaa
     3721 tagatcaatt gccaaattca tcgtgcatgt gtagatagac ataaccaacg acctccttga
     3781 ttctattatg aaatacgcga aatagaaaat gttttcttaa tcgtcccaac acaaagaaaa
     3841 cctttacatt tataaactcg tcgtgctttt tttaactaac ttttggtgaa agaaggaaag
     3901 aatatgaatc acaaattaag tgaaacagag cagtacctct ggaactttat cgaacacctc
     3961 atcctagaga tccctaacta ttccatcgtg aaactaagtg aacaagcaaa tgtctcaaca
     4021 gccaccattg tgcggacaat gaaaaagaaa ggctatgaag ggtttacttc gtttaagcat
     4081 catctaaaag aaggagaaaa caagaacatc aatttttcat ttttagataa agtagacaaa
     4141 ggaatccgcc gagccattct aaaaaatgaa caagaagtgg tacgtacaat caatatgttg
     4201 gaaatcggga acattgaaga tgcaatacaa agaatcaaat ttgctgaccg ggtttttgtc
     4261 tttgcccgag gattttcaga aatgattggc caagaaatgc tggtaaaact tcaattaacg
     4321 ggaaaaaatt gtcagatgca tactgatcca gaaattatca aaagcatcag tcggaaacta
     4381 actaagaaag acagcgttat ttttgtttct ttgaacggtg agaaaacaaa taaaaacatc
     4441 aaaaataaga tttatgtctt tatttttcaa ttttttaaat
//
LOCUS       DANJJW010000158         4473 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000158 DANJJW010000000
VERSION     DANJJW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            321..1052
                     /locus_tag="PWS82_002702"
     CDS             321..1052
                     /locus_tag="PWS82_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0907759.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="PWS82_002703"
     CDS             1197..3224
                     /locus_tag="PWS82_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0907760.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="PWS82_002704"
     CDS             3332..4429
                     /locus_tag="PWS82_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0907761.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DANJJW010000159         4434 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000159 DANJJW010000000
VERSION     DANJJW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(543..2048)
                     /locus_tag="PWS82_002705"
     CDS             complement(543..2048)
                     /locus_tag="PWS82_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0907762.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PWS82_002706"
     CDS             complement(2045..2731)
                     /locus_tag="PWS82_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0907763.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWS82_002707"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWS82_002707"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL0907764.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DANJJW010000160         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000160 DANJJW010000000
VERSION     DANJJW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            690..1658
                     /locus_tag="PWS82_002708"
     CDS             690..1658
                     /locus_tag="PWS82_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0907765.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWS82_002709"
     CDS             1669..2484
                     /locus_tag="PWS82_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL0907766.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWS82_002710"
     CDS             2552..3262
                     /locus_tag="PWS82_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907767.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWS82_002711"
     CDS             3298..>4425
                     /locus_tag="PWS82_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL0907768.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANJJW010000161         4412 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000161 DANJJW010000000
VERSION     DANJJW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1306
                     /locus_tag="PWS82_002712"
     CDS             <1..1306
                     /locus_tag="PWS82_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907769.1"
                     /translation="FKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1583..3643
                     /locus_tag="PWS82_002713"
     CDS             1583..3643
                     /locus_tag="PWS82_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL0907770.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            3726..4295
                     /locus_tag="PWS82_002714"
     CDS             3726..4295
                     /locus_tag="PWS82_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907771.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1588 a          743 c          817 g         1264 t
ORIGIN      
        1 attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg tcgaacgtct
       61 gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa aaattgctcg
      121 actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag agaatcattt
      181 tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc ttgaacagca
      241 tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac ggaacgtatt
      301 atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag aaagcgtcat
      361 gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa aactgtctgc
      421 aacaaaacga atcaaattca atttgctaac cactattagt ttgttgaggg cctatgggaa
      481 tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag aaggaatgaa
      541 gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc agatcattga
      601 tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat tagatggcgc
      661 atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa aaatgtcagt
      721 ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa gactatatat
      781 acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga tattcgactt
      841 tgagatgact agctttacgt ctaaaaagca gctacagttt ttcttcgaga cgtatccaat
      901 attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct tgggaaaaga
      961 tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg gtatttgtcc
     1021 ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag gatctgctta
     1081 taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg tgattgagag
     1141 tcgaatcact tctcgtacga atatttattt atctgattgt ttacttgaaa aatttaataa
     1201 agaacagata atttggaaaa atccaccgac tccagatgac tgggaatatt ttggaaatac
     1261 catcatcaga gaaaaaaata gacatttaac tgaaaaaatc agctagtatt tatggatggc
     1321 agctttgtct cacatattta ttagctgatt tatgtttttt tcaaagaaaa ggtaactaga
     1381 tataactaga tataactaga tatattactt aacaaaatta aaaagtacat aagttattat
     1441 tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag tccatcttct
     1501 tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa
     1561 gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca
     1621 cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat
     1681 gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac
     1741 gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac
     1801 tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta
     1861 taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt
     1921 atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc
     1981 tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat
     2041 aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga
     2101 aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag
     2161 gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa
     2221 tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat
     2281 tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat
     2341 caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga
     2401 atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac
     2461 agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac
     2521 atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac
     2581 tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat
     2641 gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc
     2701 aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat
     2761 gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc
     2821 agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat
     2881 accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa
     2941 caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga
     3001 tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg
     3061 tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca
     3121 gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagacg
     3181 agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa
     3241 agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa
     3301 taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat
     3361 tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga
     3421 tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc
     3481 caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg
     3541 tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt
     3601 atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt
     3661 tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata
     3721 gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac
     3781 aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag
     3841 atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag
     3901 cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt
     3961 tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat
     4021 caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa
     4081 aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg
     4141 acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa
     4201 aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa
     4261 aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt
     4321 attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc
     4381 ggataactaa tggcatgcca tacattcaaa aa
//
LOCUS       DANJJW010000162         4396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000162 DANJJW010000000
VERSION     DANJJW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..93
                     /locus_tag="PWS82_002715"
     CDS             <1..93
                     /locus_tag="PWS82_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0907772.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="PWS82_002716"
     CDS             complement(151..1371)
                     /locus_tag="PWS82_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL0907773.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="PWS82_002717"
     CDS             complement(1391..2002)
                     /locus_tag="PWS82_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0907774.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="PWS82_002718"
     CDS             complement(2045..2977)
                     /locus_tag="PWS82_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL0907775.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="PWS82_002719"
     CDS             complement(3398..4258)
                     /locus_tag="PWS82_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0907776.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DANJJW010000163         4378 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000163 DANJJW010000000
VERSION     DANJJW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..924
                     /locus_tag="PWS82_002720"
     CDS             <1..924
                     /locus_tag="PWS82_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL0907777.1"
                     /translation="FKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFF
                     TGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDI
                     RNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDAL
                     IQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVK
                     EIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSD
                     SSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            941..1375
                     /locus_tag="PWS82_002721"
     CDS             941..1375
                     /locus_tag="PWS82_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907778.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1992..>4378
                     /gene="esp"
                     /locus_tag="PWS82_002722"
     CDS             1992..>4378
                     /gene="esp"
                     /locus_tag="PWS82_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL0907779.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1610 a          606 c          827 g         1335 t
ORIGIN      
        1 tttaaactaa aaatgaacaa tttgaatgtt gcaatggctg atagagaacg actctataat
       61 tcattgcaaa atttgacttt gtattcttta ggagacattc gagatattct gattttagtt
      121 cattattttt ttactggaaa aatagaagat ctattccatg aaccgttaat tgaatataca
      181 gaaaatctaa gtaaaactat cgagcaaatc aaaatagata atctgttatc acaaaattac
      241 gatccagaaa tatatctgtt tctttatgaa aataaaatac tggaatatgt taaaaatgga
      301 gatattcgaa atttagaaaa catggttttt aatctgagta atggaataat tccaagtgtt
      361 agtggagata cgattcggtc tgaaaaaaat tattctatca ttgtttttga aaagttagca
      421 caaacgtcta taacattagg aatggatata atagaagcgt atcaaagtag agatgctttg
      481 atacaagaaa acgaattagc tgtatcgtta cctgaggtct taaaagtgag ggattctggg
      541 attgtttatt atactaagga aatcggaaaa actaagatag agcatctttc tcccttaatt
      601 tcttctgtcg ttcaattcat cggcttaaat atctataaaa gaattactgt aaaggaaata
      661 gctaattatt tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat
      721 attacgattt ataattatat ctctaagcga aaaatttcta cggctaaaat aatgttgaaa
      781 tcaaaccata ctatcagtga agtttcttta ggtttaggat tttcagattc ttcacacttt
      841 tcgagagtat tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt aggtttagtg
      901 gacaatttca acaacattag ctgagaggtt ttattttatt atgagactat atcatttacg
      961 tagtttggag gagttgagga aattggattt taacaaaaaa tttttttggg aaatttctga
     1021 tttgagttgt ttcgtagaat ctgagcgtct aagtaatggt aataatagtg tgttaaatga
     1081 atatgacaaa gtagtaatta ataaagtaaa aagtttatgt gaagaagcta gagaatctat
     1141 cagtcatatt aatggtagca attttaaaca aacattattt aatctgatta agctagatgc
     1201 taaaatatca agttatttat tctttttgat acatgatgaa tttatggatt atcaaaaatt
     1261 ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga
     1321 aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca attaattatt
     1381 catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat
     1441 aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg
     1501 ttgataactc caatcattcg tttaagattc taggaatcat tcagaaatgc tgtgaaaccg
     1561 atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct
     1621 ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgttattt
     1681 gaatactctg aagtttaatc taagaaaggg taatattttg aaaacatgct tcatcgcata
     1741 gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg
     1801 aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta
     1861 atgttcatga aattctctaa gtaaattagt taacataagg ttagtaagat tcctattctg
     1921 tgaataatac gtatcagatt tatcttttat tcttactagg aaagtaatta ttaagggagt
     1981 tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac
     2041 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg
     2101 ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca
     2161 gcccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
     2221 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
     2281 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
     2341 aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat
     2401 tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac
     2461 ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt
     2521 atattcgtgc aagtgatgga ttatttgctg gggcagaaac aaccgatacg caaaccgctc
     2581 ctaatggggt aattacgttt gaaaagagcc tagatgaaat ttttaaagag ataggcatag
     2641 acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg
     2701 ataaaattgt accgggtatt gatagtacgg gctacttttt aacagattca gatgatttct
     2761 ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg
     2821 caaaatatga gaaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg
     2881 gagctataat cttagatcat aagttaacga aaaattataa tttctcatat tcatcttccg
     2941 caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag
     3001 cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcgtttgaat
     3061 ccggaacaaa agtagctgat ttagcaatcg agcgacggga aggtcatgaa aattatggca
     3121 taggttcaat aactaataat gatttaacta aacttatcga ttttgctaat gcaagtccac
     3181 gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag
     3241 atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg
     3301 attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt
     3361 attatttgca agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca
     3421 cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta
     3481 aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata
     3541 aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa
     3601 tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag
     3661 atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg
     3721 ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag
     3781 atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga
     3841 aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga
     3901 tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg
     3961 aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa
     4021 aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca atttcagatg
     4081 tctataataa gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt gttacattca
     4141 ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg
     4201 tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt
     4261 atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtaaccttc accgcagggg
     4321 aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagctt
//
LOCUS       DANJJW010000164         4345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000164 DANJJW010000000
VERSION     DANJJW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(387..1853)
                     /locus_tag="PWS82_002723"
     CDS             complement(387..1853)
                     /locus_tag="PWS82_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0907780.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="PWS82_002724"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="PWS82_002724"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0907781.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DANJJW010000165         4324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000165 DANJJW010000000
VERSION     DANJJW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..281)
                     /locus_tag="PWS82_002725"
     CDS             complement(<1..281)
                     /locus_tag="PWS82_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0907782.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="PWS82_002726"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="PWS82_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="PWS82_002727"
     CDS             complement(2359..2721)
                     /locus_tag="PWS82_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907783.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="PWS82_002728"
     CDS             complement(2725..3063)
                     /locus_tag="PWS82_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907784.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="PWS82_002729"
     CDS             complement(3042..3461)
                     /locus_tag="PWS82_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907785.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="PWS82_002730"
     CDS             complement(3454..3687)
                     /locus_tag="PWS82_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907786.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="PWS82_002731"
     CDS             complement(3684..>4324)
                     /locus_tag="PWS82_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0907787.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DANJJW010000166         4308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000166 DANJJW010000000
VERSION     DANJJW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            594..926
                     /locus_tag="PWS82_002732"
     CDS             594..926
                     /locus_tag="PWS82_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907788.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            1051..1308
                     /locus_tag="PWS82_002733"
     CDS             1051..1308
                     /locus_tag="PWS82_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL0907789.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1540..1944
                     /locus_tag="PWS82_002734"
     CDS             1540..1944
                     /locus_tag="PWS82_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL0907790.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1963..>4308
                     /locus_tag="PWS82_002735"
     CDS             1963..>4308
                     /locus_tag="PWS82_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0907791.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQ"
BASE COUNT         1585 a          583 c          788 g         1352 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta
     4141 aatccacatc tagaagatgt agaattcttt tcaattgaag atatgatttt aggaatgaat
     4201 ataagtaaat tctccaatca attgaataga cactttaatg ataatgaact gaaagttttg
     4261 aacaataata ttctgaagaa ttttagtcta acgaatttaa tggagcaa
//
LOCUS       DANJJW010000167         4122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000167 DANJJW010000000
VERSION     DANJJW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(638..1594)
                     /locus_tag="PWS82_002736"
     CDS             complement(638..1594)
                     /locus_tag="PWS82_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907792.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1703..1948)
                     /locus_tag="PWS82_002737"
                     /pseudo
     CDS             complement(1703..1948)
                     /locus_tag="PWS82_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2093..2515)
                     /locus_tag="PWS82_002738"
     CDS             complement(2093..2515)
                     /locus_tag="PWS82_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907793.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2695..3066)
                     /locus_tag="PWS82_002739"
     CDS             complement(2695..3066)
                     /locus_tag="PWS82_002739"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0907794.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(3400..3663)
                     /locus_tag="PWS82_002740"
     CDS             complement(3400..3663)
                     /locus_tag="PWS82_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907795.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1261 a          851 c          632 g         1378 t
ORIGIN      
        1 gcaaaagaaa acttataatc aaataatatc aacatttgat cacataattt gagattgaat
       61 gctgatttac ctatgtttta cgtaatggtc tttagttatt tttgctcata aaatgcataa
      121 aacacgcgtt tttatgtgtg cttgaaattt cttggctcta taatttctaa gactgataaa
      181 aatattctat cggttctttt tgcaaataaa aaaacatata gaagctccat tagattgaat
      241 caccacaaca caatcaaagg agcattccgt atgcttgatt ttcattctat aaaactactt
      301 ttaggaatca aagaaaatca caacggttca aactaaaagc aagaatatcc gaagtaccat
      361 tgtttcatcg atcatgagaa aagcattcac ctctgtctgc agatgttctt atatcgctct
      421 gtcagtccta ttaataaagt actgttttgt tttcaaaaaa actattacga gtacttttta
      481 agagaatgcc ttgcaaatat tcatcacaca ccgttatata agtcaaatag tttctttcag
      541 tcgtcaagaa aagattctcc tgtccttggc attatcagtt aagtagcaac ttaattctcg
      601 gacttgttct actctagcaa aatttatttt catcaactta cgtccaaatg tttttttgaa
      661 taattacttc atcgcgggtc tggaagactt gcctcattcg ataagtcact agcttttggc
      721 gtccttcagg atttctagta tacaccaact gacatttggg cagcgacgat tgatttaatt
      781 cgtttagaaa aaggttagcg atttgcttat ttcggccaaa gcgttctggt accgtcagaa
      841 ctatcttttt cccttcgatt aaataccgcg gattctccag cggtgctaaa agctccctca
      901 tagcttcagt aaaaagctgt ccttctcgat agccagcatt ctcaagatag catctaaatt
      961 cctgctgatc agccgctacg attacttttc tatcactaac ttcaccaagg cgctgcaacg
     1021 tcgtttgaac gcatatggcc aagctttttt tgcgggcaca ttccttttcc catagctttt
     1081 gtcgcctacg aatttggaaa tattccagca agttaccttt aaatatagct gaccagaagc
     1141 ttagaggctc tatatttttt actactatcg gcaatgaagt ttcttgtttt tgctttacct
     1201 gatcgacttt cctaacgacg attggcatag ataggtgcca gccatcagct aaagcagtac
     1261 tccattgttc tttcaaccga cttcgttcac aagaaaaata gaacatcgac tgagtataag
     1321 tttccatcgt ggtggtcgta tattccagtg gtaaagcaaa gcgatcgcca ccactctcaa
     1381 tttttggtga agattcaaag gtcagacctg aaatattgcc caaacgccgt agcattcttt
     1441 tcaaatcagg ctgttcagac agaggaactc ccggtaatcc aatcgctaaa tgccaaatgt
     1501 tcgttacttt attttgtgca cctaggcgta agccccgccc tcgtagttgt tgtgtttgca
     1561 caaaagtgga agcttgatta gccaagatca acagattaat tgcaggacaa tcccagcctt
     1621 ctcctagcaa actaacggtt cctaccaaaa tattcaactt cttttcggtt aataggcgag
     1681 taactgcttg catcaatgct tggcggtttt tgtcatccac cgtcaaataa aaataacccg
     1741 acatataagg atgttcattc atacccgtcg ctagttctgc cagctcaaca gttactagca
     1801 ttttcggtaa cagtaagaac tctccacaga tggcaccgat ttgctgagtg ttacctaata
     1861 gacgaacaac ttcatcaaac aaaggaacca cgccataagc taaggtacca tcactgcctg
     1921 ctaatacctc cttgcaaata cgatccatca aaatgaccgc tgtcagttct tgcttcagct
     1981 gaacttcttc ttttaacaaa atttgacaaa ctgcttgctt tttctcagga atcgcctgac
     2041 taccaacagt gtctggatac tctggaaaca aatgcagcga ctcatcaaac aatcatccag
     2101 cttttttcaa ttgataaaaa ataggcaatt ccggttgatc ttcaaacatc tgctgataaa
     2161 tagtgatcag ctctttttta cctaaagatg gaactgaagc ttttttacct tgctgcttag
     2221 aactgcctaa tagctgccaa tgttcgggta tcaattcata gttatgatga ttcaagtaaa
     2281 ttaatgcact agtgaacagc tcaaaatttt cataaatttc cttcgaatgc tcaagcggat
     2341 gctgaacaaa ttctaaagaa agcaaataat ccaccgtctc ttggctttcc attaacgctt
     2401 ccaccagctc attttgtttc tctaaaaagg taaaataatg ctgagtagtt tcctgattag
     2461 ccggaatcaa atatagaaaa tcctgatgag gacatagaac ctcctccttt accaactcgg
     2521 gaatgctaat ttcttcgtca atcaggccat tttaactcaa tatattgctt ccactccgtc
     2581 tgagagctat caatcggagg cgtggctgtc aaggaaatcg tcaacagatt cggttgtttt
     2641 ttcttcaagt caatcaacaa ttcaccccag ctttttttta aatgatgagc ttcatcaaaa
     2701 aactaacagc tccagctgat caccataagt tggctcctcc ttcagtcggt tgtaaatggt
     2761 ctgatacgtt tccactgtaa tatgactagg atgttcaatc gccccagaaa gatagcaact
     2821 atctgctgga tttactaaga aatattcctc gattgttgtt aaccactgtt tcttcaatat
     2881 tagggaagga accaaaatta aggttttaca atccaaacgt cggatcatct caattcctaa
     2941 aaaggtcttg ccagcccctg gtggagccac tagatggact gaattgttat ttaaataagt
     3001 agcaaagtta gtcaatatac gcttctgata gctacgccac tgaatttttt catcagatag
     3061 ccacatccca ggtccccctc tcacgtattt tctctatcat aatctaaatg aatacagcaa
     3121 aaaaaagatt tcttcaagaa tatgtgtttt tatagtaata ttttacttac tctaaactca
     3181 aatgaaaagg taaaaaaact ggtagtacaa tcttttaaat tctcattgtt ttgtacgttc
     3241 tttactatca aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt tttagctcta
     3301 taatttctaa gactgataga aaatcattct gtttttgaag tcttttttca taaatactac
     3361 ttgatttatt gtgtatattg tataaaagat tgttcataat tattttttat aattaaaatt
     3421 tggtattgat gaccagcgat tccgtatata atgcgcaatc aaattaagct ttctgtctct
     3481 ggtaaatatt cctgttctgt tttcttgtac tcgtaagatt tcttcactta tcgcaaaatt
     3541 cataaaattc cacacttttt cataaacaaa atgtacacat ttatctatta cttcataata
     3601 gtctacttga tattcttctg taaacatggt tgctactgta tctgcaccat atttgataaa
     3661 catttgggct actcccttaa attgagaaaa gcccattagt ttatctacta atacttctaa
     3721 aatttcttgc tttcttcttt aacatcgtcc cgtaaggaat catgtagtat gctggaggaa
     3781 gttcattttc taaataattg tatcgcttca attgctcaaa ggctattctc tacaattaat
     3841 tacaccaatt actttatctt cacttatata agatttctaa acatttttcg tcaaatgaag
     3901 agaaaatatt tttttaaatg atcttgctca agatttgtca acgaatcttt gaattcgttt
     3961 tgaagttctg aggaaggtat catttaaaaa ctcaatctga ctttcatcat ttcgtttact
     4021 cccattttga tcaccttcta ccaacaattt attaaagtga ttatactagg tttttatttt
     4081 tttaattcac aattgtttat tcacttcatg aacaattaaa ca
//
LOCUS       DANJJW010000168         4112 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000168 DANJJW010000000
VERSION     DANJJW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(567..932)
                     /locus_tag="PWS82_002741"
     CDS             complement(567..932)
                     /locus_tag="PWS82_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907796.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1008..1253)
                     /locus_tag="PWS82_002742"
     CDS             complement(1008..1253)
                     /locus_tag="PWS82_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0907797.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1323..1577)
                     /locus_tag="PWS82_002743"
     CDS             complement(1323..1577)
                     /locus_tag="PWS82_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907798.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1741..2064)
                     /locus_tag="PWS82_002744"
     CDS             complement(1741..2064)
                     /locus_tag="PWS82_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907799.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2251..2324
                     /locus_tag="PWS82_002745"
     tRNA            2251..2324
                     /locus_tag="PWS82_002745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2285..2287,aa:Ile,seq:cat)
     gene            complement(2709..3206)
                     /locus_tag="PWS82_002746"
     CDS             complement(2709..3206)
                     /locus_tag="PWS82_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0907800.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3246..4055)
                     /locus_tag="PWS82_002747"
     CDS             complement(3246..4055)
                     /locus_tag="PWS82_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0907801.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1374 a          726 c          723 g         1289 t
ORIGIN      
        1 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
       61 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
      121 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      181 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      241 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      301 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      361 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      421 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      481 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      541 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      601 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      661 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      721 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      781 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      841 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      901 tttgaaatca atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt
      961 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     1021 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1081 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1141 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1201 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1261 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     1321 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     1381 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     1441 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     1501 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     1561 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     1621 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     1681 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     1741 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     1801 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     1861 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     1921 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     1981 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     2041 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     2101 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     2161 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     2221 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     2281 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     2341 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     2401 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     2461 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     2521 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     2581 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     2641 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     2701 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     2761 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     2821 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     2881 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     2941 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     3001 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     3061 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     3121 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     3181 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     3241 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     3301 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     3361 ctgtaattca acatagcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     3421 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     3481 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     3541 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     3601 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     3661 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     3721 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     3781 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     3841 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     3901 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     3961 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     4021 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     4081 tatctatttt cctgttacct taagattaaa tg
//
LOCUS       DANJJW010000169         4026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000169 DANJJW010000000
VERSION     DANJJW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(232..381)
                     /locus_tag="PWS82_002748"
     CDS             complement(232..381)
                     /locus_tag="PWS82_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907802.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="PWS82_002749"
     CDS             843..1055
                     /locus_tag="PWS82_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907803.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="PWS82_002750"
     CDS             complement(1333..3132)
                     /locus_tag="PWS82_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0907804.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="PWS82_002751"
     CDS             complement(3256..3852)
                     /locus_tag="PWS82_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0907805.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          689 g         1183 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataacttgt
     4021 tttttt
//
LOCUS       DANJJW010000170         4018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000170 DANJJW010000000
VERSION     DANJJW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1543
                     /locus_tag="PWS82_002752"
     CDS             <1..1543
                     /locus_tag="PWS82_002752"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL0907806.1"
                     /translation="KMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSK
                     QTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFV
                     QLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAY
                     EFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNV
                     RNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYT
                     FDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAI
                     VLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDV
                     LFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIP
                     RYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEV
                     FKHEQ"
     gene            1533..2750
                     /locus_tag="PWS82_002753"
     CDS             1533..2750
                     /locus_tag="PWS82_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0907807.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2763..>4018
                     /locus_tag="PWS82_002754"
     CDS             2763..>4018
                     /locus_tag="PWS82_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0907808.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1390 a          681 c          835 g         1112 t
ORIGIN      
        1 taaaatggat gcgtcagaat ataaaaatta tttattggga ctgattttct ataaatattt
       61 atccgatcga ttgttggaac aagtggttct tttagcagat gaatccttag aggaatatga
      121 cacagtaagc aaacaaacaa tgttgtatcg tgaattactt tcagatgaag aatcaaaaga
      181 agatctgatt gccacaatcg ttgatatttt gggatatgca attgcaccag agtatctttt
      241 caacgtctta gcggatcaag caaaacaagc aacgttccaa ttgaacgatc taaacaaagc
      301 ctttgtgcaa ttggcatcta cctacaatca atttaatggg ttatttgatg atgtggatct
      361 ccaatcaaaa aaactaggaa cagatgagca acaacgaaat gtgacgatta ccgaagtcat
      421 caagaaattg aatgatgtag atgttttaga acacgatggg gacgtgatcg gggatgcgta
      481 tgagtttctg atcagtcaat ttgcttcaga agcagggaag aaagccggag aattttacac
      541 acctcatatg gtttctgata tgatggcgca aatcgtaacg ctcgatcaaa aggaacgacg
      601 attctttagt gtttttgatc caacgatggg ttcaggttca ttgatgttaa acgtacgtaa
      661 ctacttaaca cacccagata acgttaaata tcatgggcaa gaactaaata caacgacata
      721 caatctagca aaaatgaact tgattttaca tggtgtcgac gccgaagaaa tgaatctccg
      781 caatggggat acgttgaata aggactggcc aacagatgaa ccttacacat ttgatgcagt
      841 agtaatgaac ccaccttatt cggctaattg gtcagcagat acgactttct tagatgattc
      901 tcggtttaat cgttatggga aattagcgcc aaaatccaaa gcagattttg cgtttcttct
      961 tcatgggttc tatcatttga aggaaacagg taccatggcc atcgtcttac cacacggagt
     1021 tttattccgt ggcgctgcag aaggggtcat ccgtcaaaaa ttactagaag atggcagtat
     1081 ttatgctgtg atcggtatgc ctgcgaactt gttctttggg acatcgattc caacgacggt
     1141 gattgtgttg aagaagaatc gtcaaactcg agatgtatta ttcattgatg ctagtcgtga
     1201 atttgttaaa gggaaaaatc aaaataaact atcggaagaa aacattcaaa aaatcctcga
     1261 aacctacgct gaaagaaaag atgtcgagaa atacgctcat ttggcaacct ttgatgaaat
     1321 caaagaaaat gactataact taaatatccc acgctatgta gatactttcg aagaagaaga
     1381 accaattgat atggttcatg tcggaaatga catcaagaag atacgacaag aacagcaagt
     1441 gctagaaaaa aaacttttag aagctatctc ttccctgcaa acaacacctg aaaatgaagc
     1501 gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa tgatacacaa ccagaaatcc
     1561 gtttcccagg atttacagat gattgggaac agaggaagtt gggggacttc gttgtagatt
     1621 atgttgaaaa gacaagtgta caaaatcaat ttcctatgct aacttcatct caacaaaaag
     1681 ggatagtttt gcaagaagac tattttgcaa atcggcaagt aactacagaa aataatattg
     1741 gttactttgt actacctaga ggatatttca cgttccgtag tcgaagtgat aatgatgtgt
     1801 ttgtgtttaa ccgtaatgac attattgata gagggattat aagctatttt tatcctgtat
     1861 ttactttaaa atctgctgat tccgacttct tcttaagacg tattaacaat ggaatccaac
     1921 gacaattatc tattcaagca gagggaacag gtcaacatgt tctgtctcta aaaaagttta
     1981 aaaatatagt agcaatgttt cctagtgagg aagaacaaca aaaaatcggc accttcttca
     2041 aacaacttga cgacacgatc gctcttcatc agcgtaagtt agatttgctg aaggaaacga
     2101 aaaaaggatt cttacaaaaa atgttccctg aaaacggagc aaaagtgcca gaaatccggt
     2161 ttcctggttt tacggaagat tgggaagagc gtaagttggg agaattaggt aaaactcaat
     2221 cgggaattgg atttcctgat gccgagcaag gtggttctga aggtactcca ttttttaaag
     2281 tttccgacat gaataacatt ggtaatgagt atgaaatgag aaatgcaaat cattatgtat
     2341 ctaatgagca aattgagcgt aaaaaatgga agccaataaa agatgtgcca gcgattatat
     2401 ttgctaaagt tggcgcagca ataatgctta atagaaaacg tttggtcaca agccctttct
     2461 taatagataa caataccatg gcttatttat ttaataatac ttgggatatc tattttggta
     2521 aaatattgtt tgaaactata aatttgccaa gatactccca agtaggggca ttgccttctt
     2581 ataacagttc tgatattgag aatatcagtg ttaaggttcc tgtaaaggat gaacaacaaa
     2641 aaatcggtac tttcttcaaa cagctagatg atacgatcgc tcttcatcaa cgtaagttag
     2701 atttactaaa agaaacgaaa aaaagcttct tacaaaagat gtttgtctag gagggaagaa
     2761 gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga acagttgatt cgtgtacttg
     2821 gagaagggca taatcagtgg acgtatcgtc cggatctaaa atcagaagaa gacctttggg
     2881 tcaatttacg ccaaaaaatt atctcaaata atcaagcaga actgaatgat tttcctttga
     2941 ccgataaaga atttgaaacg atcaaaacgg aattactgct gcgaacgaaa accccatttg
     3001 atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg gatcacgatt gaacgggaag
     3061 attcgcaatt gggttctgtc tcacttatcc tttattccaa tcaagacatt ggtggaggaa
     3121 tctctactta tgaagtggtc catcaaattg caaaacgtgg aagtaacata gaagcacggg
     3181 atcgacgttt tgacgtcacc ttattaatca atggattacc gattgtccaa atcgagctga
     3241 aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa ccaaatcaaa aaatatgcag
     3301 aagaaggtat gtttcggaac aatatctttt cgaccttaca gttgtttgtg gtttccaatg
     3361 aacaaaccac tcggtatttt gcaaatgcct taccgaaaga cttgcaccct aagtttttat
     3421 ttagttggcg aacgaaagac aatgaaaaag tagaaaactt atatgagttt tgtaaacaag
     3481 tgttaaatat cccggatgct catcgtttga ttgcggatta tacaatcgtc agtgaagatc
     3541 aagacaataa aaccctcatg gtgttacatc cttatcaagt acatgcgatt caagccttgt
     3601 ttattgccgc aaataaacat caatcagggt atgtgtggca tgcgactggt tcaggaaaga
     3661 cgttgactag ttttgtatct actaagcttt tagcacgaaa atcggggatc gatcgaacga
     3721 tcatgttagt ggaccgcaaa gatttagata atcaaaccac cacagagttt acaaaatttg
     3781 cttcggaatt taatactggg atttcttccg ggaacgccaa agcgaatagc ttgatcgttg
     3841 gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc agatgccaat gcaaatgttg
     3901 taatcattac aacccgtcaa aaactagatg cggcgttgaa atatgcaaag aaacaagaag
     3961 agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca acatatcgtg tttgtagt
//
LOCUS       DANJJW010000171         3953 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000171 DANJJW010000000
VERSION     DANJJW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1008
                     /locus_tag="PWS82_002755"
     CDS             <1..1008
                     /locus_tag="PWS82_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907809.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="PWS82_002756"
     CDS             1025..1174
                     /locus_tag="PWS82_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907810.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="PWS82_002757"
     CDS             1171..1617
                     /locus_tag="PWS82_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907811.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="PWS82_002758"
     CDS             1619..1756
                     /locus_tag="PWS82_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0907812.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="PWS82_002759"
     CDS             1794..2087
                     /locus_tag="PWS82_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907813.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="PWS82_002760"
     CDS             2084..2308
                     /locus_tag="PWS82_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0907814.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="PWS82_002761"
     CDS             2305..3330
                     /locus_tag="PWS82_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0907815.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="PWS82_002762"
     tRNA            3520..3593
                     /locus_tag="PWS82_002762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1375 a          672 c          809 g         1097 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttata attggagtgg tag
//
LOCUS       DANJJW010000172         3944 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000172 DANJJW010000000
VERSION     DANJJW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1383
                     /locus_tag="PWS82_002763"
     CDS             <1..1383
                     /locus_tag="PWS82_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907816.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
     gene            1397..1546
                     /locus_tag="PWS82_002764"
     CDS             1397..1546
                     /locus_tag="PWS82_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907817.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1543..1989
                     /locus_tag="PWS82_002765"
     CDS             1543..1989
                     /locus_tag="PWS82_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907818.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1991..2128
                     /locus_tag="PWS82_002766"
     CDS             1991..2128
                     /locus_tag="PWS82_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0907819.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            2165..2458
                     /locus_tag="PWS82_002767"
     CDS             2165..2458
                     /locus_tag="PWS82_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907820.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2455..2679
                     /locus_tag="PWS82_002768"
     CDS             2455..2679
                     /locus_tag="PWS82_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0907821.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2676..3695
                     /locus_tag="PWS82_002769"
     CDS             2676..3695
                     /locus_tag="PWS82_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL0907822.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1371 a          699 c          820 g         1054 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taacttacta
      481 gatgaccctt actgggtagg taaaacatcg ttgggtgaga atattgcaaa taaaggtacg
      541 acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga agaagagctt
      601 ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      661 gtagcatata attattggaa cgttaatttt ggagaactaa aaccagttga gggattgaca
      721 gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc taaagacctt
      781 cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc aaattgactg gctcaaaatt
      841 gaaaaaggcg acacccgaac cccgaatatt agtcagttta aatactttgg tgaaggattg
      901 aaagatagca acaatccgaa cgactacagc tgggacatca cgcctgaata tactgaaaaa
      961 ggcttgaatg atacggttag tttgactgaa ccgcaatcca tcgaaggatt gaaaaatttc
     1021 gaagatggta ttcaatcaaa aggaaaatct gtattgacat cagacgacaa caaatatgaa
     1081 gtcgtaacct taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
     1141 aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca
     1201 acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc
     1261 tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt
     1321 aaatggcgaa gaaactcaag ttatggatca gcttattcat ttgttatcac ttattcaatt
     1381 taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca
     1441 aatcattgtg aatgacgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
     1501 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
     1561 tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct tgtatccgtt
     1621 ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc gctaacgatt
     1681 gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa cgcgttagat
     1741 cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga aaacggcaac
     1801 ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca ggaaaatact
     1861 gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat gcttgcttcg
     1921 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaagga
     1981 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
     2041 tgacgatcaa gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat
     2101 cattaataaa gataagagcg catcttaatt gatgcgtttt tatttaaggt aaaggagttg
     2161 tcacatgatt aatttagggg aatggggaat gatagcagga tcaataaccg ctatcgtttc
     2221 tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta ctgagactct
     2281 ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
     2341 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
     2401 aaaattggtg gaacacacgc aacaaatcaa aactttgttt agggagagaa gaaaatgaat
     2461 aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc actagctact
     2521 ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg tgttgtcatc
     2581 attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg aacctacaaa
     2641 atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt actattactg
     2701 cgatgagcct gttaacggct ctttttttat tgccaattaa cggatttgcc tatactatca
     2761 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtata
     2821 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
     2881 aacgttcatg gactagcgct tatactgctt atattgtggg agacggcgga attgtttacc
     2941 aagtcggaca acctggttat gtacagtacg gtgctggttc atatgctaat gccaatagtc
     3001 ctgtgcagat tgagttacaa cacacacatg ataaagcaac ttttgaaaaa aactataaag
     3061 catacgttga attggctaga gattcagcaa tgaaatatgg tattccatta acattagaca
     3121 cgccttataa ccaaccagga atcaaatcac atttatgggt aactcaaaac atttggggcg
     3181 atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa aaactggctt
     3241 acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac aagcctgtca
     3301 ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg aaccattctc
     3361 acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg
     3421 ctaactataa gtacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
     3481 gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat acttctggaa
     3541 atgttggcta tcatgtatct tttaacatgc gtaacttccc taataagaaa gtctatgtca
     3601 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
     3661 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcatt
     3721 acataactat attgacaact ataaaaatca ttcgataaaa tagtgatgtt atcgcatatc
     3781 ttcactatca cccataatag tcacactcca agctatgcga taacaggttt gttgccacac
     3841 attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt
     3901 ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagt
//
LOCUS       DANJJW010000173         3876 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000173 DANJJW010000000
VERSION     DANJJW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            158..2257
                     /locus_tag="PWS82_002770"
     CDS             158..2257
                     /locus_tag="PWS82_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0907823.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="PWS82_002771"
     CDS             2453..3316
                     /locus_tag="PWS82_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0907824.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="PWS82_002772"
     CDS             3421..>3876
                     /locus_tag="PWS82_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0907825.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DANJJW010000174         3853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000174 DANJJW010000000
VERSION     DANJJW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(39..332)
                     /gene="rpmA"
                     /locus_tag="PWS82_002773"
     CDS             complement(39..332)
                     /gene="rpmA"
                     /locus_tag="PWS82_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL0907826.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(345..689)
                     /locus_tag="PWS82_002774"
     CDS             complement(345..689)
                     /locus_tag="PWS82_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL0907827.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(716..1024)
                     /gene="rplU"
                     /locus_tag="PWS82_002775"
     CDS             complement(716..1024)
                     /gene="rplU"
                     /locus_tag="PWS82_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL0907828.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1044..1120)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1306..1947
                     /locus_tag="PWS82_002776"
     CDS             1306..1947
                     /locus_tag="PWS82_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0907829.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2009..3640)
                     /locus_tag="PWS82_002777"
     CDS             complement(2009..3640)
                     /locus_tag="PWS82_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL0907830.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3763..>3853)
                     /locus_tag="PWS82_002778"
     CDS             complement(3763..>3853)
                     /locus_tag="PWS82_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDL0907831.1"
                     /translation="ALYGSLTSDRWILLKQGGLALLALLSIFL"
BASE COUNT         1187 a          849 c          675 g         1142 t
ORIGIN      
        1 aaaagtcttg tcacaacctc tttaatcata agattcaatt aagcttcttg agctactgga
       61 taaacagaca cttgtttttt gttacggcct ttacgttcga aacgtactac gccatcaact
      121 tttgcaaata aagtgtcgtc tccaccgata cccacgttta cacctggata gatttttgta
      181 ccgcgttgac ggtaaagaat tgatccacct gtaactgttt gtccatcagc acgtttagca
      241 cctagacgtt ttgattctga gtcacggccg ttagatgtag aaccgccacc ttttttgtgg
      301 gcaaataatt gtagattcat agataacaac atggtctgca cctcctattt atcattaatt
      361 gttttaattt ggatatattc tgagttttca gcttctatcg attgtaatcc taataaaaga
      421 ttttctaata gaatctcagc aatattgttt tgttcttgtg tcattcctga agtgacttcg
      481 gtataaagat aaccgccttc ttcttcattc atttcaacaa tcggctcaaa accggctaat
      541 gaggcaatcc cgttgacagt gctgatcgct aacgcagaaa cacccgcaca aacgatatca
      601 cttccataag gaccagcatc agcatgcccg gtcaaagtga atgaaacgat ctgacctgcg
      661 tcatttcgct taaatgttcc tttgatcata tataggcctt ctttctcaaa cgctattaag
      721 cgttgatcgc gtcgataacc acttttgtat atggttgacg atgaccttgt ttacggtgtg
      781 aatgtttttt aggtttgtat ttgaaagtaa cgactttctt ttgtttgccg tgtttttcaa
      841 cagtaccttc aacagttgca cctgcaacag ttggagcgcc tacttttgta gattctccgc
      901 ctactaagat aacttcgtca aaaacaactt tttctcctgc ttcaacgtca agtttttcaa
      961 catagattgc ttgtcccact tcaactttta cttgtttacc accagtttta ataattgcgt
     1021 acatgctata aagcacctcc ttctgatata cttagactcg ccatccgaag tgacagcata
     1081 tgctgtgctt attacttgtt ctgagcggtt gtagctgtgg agtccacaat tacaacagta
     1141 ctagcttacc agaacagcaa ccgttagtca actaaattta aaatcaaaaa gtaaaacaaa
     1201 aattcaagca atacttctgc ttttctatcc tcacagttct accaaataaa atagtcgcat
     1261 tgtgtttctt ttattcttat aataaaagaa aaaggaggag cataaatgga atacccaaaa
     1321 gaactgatac aagaagcttc tttaagaatg aacggccgtc ctgtagaagg atttatcgct
     1381 gggcaaggac ctcttcatcc taaactgatg ctcgtgggag aagccccagg gaaaacagag
     1441 attgatacgc atgttccttt tagcggtcaa gctggaaaag aattgatgca ggcactttct
     1501 tcaactggct taacacgaga agaggtgtat atcaccagtg ctgtaaggag ccgcccgtat
     1561 cgcgtgactc atcggatcaa taaacggaca caacagtcag aaactgttta tccaaatcgg
     1621 acacctactc gttccgaagt atttgcgcat gcaccaatat tggattacga actgatgaag
     1681 gtccagccaa aattaattgc gacacttgga aacattggtc ttcaacgttt acttggcaaa
     1741 gaatatacgg ttaccaaaaa tcatggtcaa ctctatacag ggcctgtctt acaattaaca
     1801 gaacaaaagg atgcctacga atggtcagaa aaaaactatc gaattcttcc actttttcat
     1861 ccagcagcta ttttctacaa tcgctccctt gtacctgtta tccatcaaga ctggcaagta
     1921 ctagcttcac ttttacagca atattaaaaa gaacagacaa gaccataaaa tcttcagttt
     1981 acagtcttgt ctgttctttt tcattgtttt aatggctaat tgcagtatat ttcgcatgta
     2041 aagctgtcaa cgcatgagca actgtttgat gttgatgcgt gaatgtatgt tcccacaagt
     2101 gagaaagttg tttttctact tctggctgca aggaagtata gataatcgtt tgtcctcttt
     2161 gctctgcttt ttcttgaaat tcatcaaaaa ctgcacgtgc cgtcaaatca atacttggta
     2221 cgcctcgcat ggagaaaatg acaccatcgt gatgatttag ttcttccaat ttatttttga
     2281 gtttttctga agacataaaa aacagtggac cactaatata cactactgtc cagttattca
     2341 gcttttccgt ttctggcagc cccattcttt gccaatcgat cgtttctaat gaaatcgtga
     2401 tagcagcgct tttgacaaca aaaaatacac agccgccgat caccccgatc acgatagcaa
     2461 tacttaaatc aaagataact gtacaaatca tcgtaaggat aaacaataga actgctgacc
     2521 aatatttatt agtaaatagc tccttgatcg tttcccattc attcatacgc caagccgtca
     2581 caatcaaaac acctgcaagt gctggcattg gaatctgtga catcactggc gcaaaaacaa
     2641 gcatggagag cagtaagaca actgcatgta tcataccagc taatctagtt tgtgcaccag
     2701 atttgatggc cacgcttgta cgggcgatgg ccgcagtagc aggaatcccg ccaaagaaag
     2761 gaagcaacag atttccaatt ccttgcgcca ttaattcttg attgctatct aagggcttat
     2821 ttgccattcg gccagccgaa gcaccacata gcaagctttc aatcattcct aataaagcaa
     2881 tgctaatagc tggaactgcc acttgttgca aggcagaaaa gctaaaatct cccatattca
     2941 aacgattact actgatcaat gtttgtggaa tcttacccac agttgctact ggtaaatgga
     3001 acaatagcat aactgctgtt gccaaaatga tagccagcag cgaactaggc attcgtttcg
     3061 cccatttttt tggatagata aacatcccta aaatgactag ccctcccatg atcaaagagg
     3121 ctgtgtttat cggaaatcct atcgtttgat aactacttag tttttcaata acgtttgtac
     3181 cttccgagtg aacaccaaat aaattatcga tctgacctaa tgcaataatg atagcaatac
     3241 ctgaagtaaa ccccgtaatt acgggtgctg ggataaaaga agtcagtgtt cctaatcgga
     3301 aaagtcctgc tgcaagtaaa aatagacccg ctaaaaaagt agcaagcaat acaccttgca
     3361 ttccctttgt tgccgcaata gacatcaaga tagctgccat cgcacctgtc ggacctgata
     3421 tttgataaaa accgcctgat aaaccgccaa taacgatacc ggcaatgata gccgtaatca
     3481 aacctgcggc cgcatcagct ccgcttgata ccccaaaagc caaggctaaa ggcaatgcta
     3541 cagcagctac tgtgatccca gccaaaagat ctttctgaaa tcttgaaaga tcataacctg
     3601 aaaattcttt ttttagtgag gaaacataat ttttgaacac ctatctaact cctttttcat
     3661 gaaattttct aaaataccat tcattagaat atcatatttc atgagataaa gtaatatttt
     3721 tcacaaagtt tcctgttaga taaattctta ttttttctat atttataaga aaatagaaag
     3781 caatgccaac agagctagcc caccttgttt gagtaaaatc caacgatcgc tagtcaaact
     3841 gccatataat gca
//
LOCUS       DANJJW010000175         3820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000175 DANJJW010000000
VERSION     DANJJW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(739..1032)
                     /locus_tag="PWS82_002779"
     CDS             complement(739..1032)
                     /locus_tag="PWS82_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL0907832.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1435..2613)
                     /locus_tag="PWS82_002780"
     CDS             complement(1435..2613)
                     /locus_tag="PWS82_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0907833.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2717..3031)
                     /locus_tag="PWS82_002781"
     CDS             complement(2717..3031)
                     /locus_tag="PWS82_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907834.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3138..3356
                     /locus_tag="PWS82_002782"
     CDS             3138..3356
                     /locus_tag="PWS82_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907835.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3351..3746)
                     /locus_tag="PWS82_002783"
                     /pseudo
     CDS             complement(3351..3746)
                     /locus_tag="PWS82_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1219 a          726 c          558 g         1317 t
ORIGIN      
        1 ttttattcta aggttatttt agtcttgtcc tttaatacaa agattttatt tttaaataaa
       61 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
      121 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
      181 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
      241 gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa
      301 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
      361 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
      421 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
      481 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
      541 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
      601 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
      661 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
      721 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
      781 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
      841 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
      901 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
      961 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
     1021 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
     1081 tctggatttt ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt
     1141 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
     1201 gaaaaaactg gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca
     1261 agggttcttt ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa
     1321 agtcgttaaa gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt
     1381 tgttaaaaaa tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact
     1441 ctctaaagaa ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata
     1501 aattttcgaa gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca
     1561 gtgttccgct actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt
     1621 aattcctgag cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg
     1681 aacaccacgt ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct
     1741 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
     1801 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
     1861 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
     1921 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
     1981 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
     2041 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
     2101 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
     2161 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
     2221 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
     2281 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
     2341 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
     2401 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
     2461 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
     2521 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
     2581 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
     2641 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
     2701 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
     2761 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
     2821 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
     2881 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
     2941 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
     3001 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
     3061 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
     3121 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
     3181 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
     3241 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
     3301 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tcctagctct
     3361 tccaaagcca tttgtcgttg gtaccaaaca ctgccgagac ttttcccaat aatatcgcca
     3421 atttcacgat cattaagacc gatgaagaag gacataaaaa tgatttcttg tctttgacta
     3481 ggtaatgctg caatcgcatt tgccaactcc tcattggaaa taaagatttt cataccaagg
     3541 acaataaaag taccactact attcaaattt ggttccaaat aatagaactc tgtacctaac
     3601 cctttttgtt ccaatatttc cattgatatt tgccgttggc gaaatctagc gtacttcttt
     3661 tgaatattta acgcctcatt gcagactact ttcttacaaa aactgtcaaa cgtatgttga
     3721 atgtgggttt cctctgagtc gcccataata ttctccccct caaaaaattc attcaactcg
     3781 ttttacttct atccctttcc attattacta caatctagga
//
LOCUS       DANJJW010000176         3701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000176 DANJJW010000000
VERSION     DANJJW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..263)
                     /locus_tag="PWS82_002784"
     CDS             complement(<1..263)
                     /locus_tag="PWS82_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL0907836.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRR"
     gene            complement(235..1188)
                     /locus_tag="PWS82_002785"
     CDS             complement(235..1188)
                     /locus_tag="PWS82_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0907837.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1800..3293
                     /locus_tag="PWS82_002786"
     CDS             1800..3293
                     /locus_tag="PWS82_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL0907838.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3428..>3701
                     /locus_tag="PWS82_002787"
     CDS             3428..>3701
                     /locus_tag="PWS82_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL0907839.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1216 a          616 c          680 g         1189 t
ORIGIN      
        1 cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc gtccattgca
       61 agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg aatggttttt
      121 agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt tcgttcccct
      181 aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt cttcttatct
      241 ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta ggatatgatt
      301 ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac catgttgttc
      361 atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag cccaccatct
      421 tcgatctaca taatcattta attggatacc attctcacca tacatttcta accgatccga
      481 gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg ttttttgata
      541 gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc caatgatttg
      601 aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa taaaatcttc
      661 tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat ctgcagcagt
      721 taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga tataatcgaa
      781 attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac gacgatcagg
      841 atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag ccataatata
      901 taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga ttgctgcaga
      961 catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag tagtatttct
     1021 ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag aaacaatcgc
     1081 taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa gcattacaac
     1141 ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag ttccctccaa
     1201 tttcctatac taaatatatt tagtatactt catatactta atatattcga tatatctaat
     1261 atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat actaaatata
     1321 tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg tgcgttttgc
     1381 gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca gataattcac
     1441 tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca taaaaaaaag
     1501 agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg ttaggacatg
     1561 tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt aaaagttgat
     1621 cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact caacagaaga
     1681 acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga acacatacta
     1741 tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg ttacaatcga
     1801 tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac aaatatcgaa
     1861 acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct atctttgcgt
     1921 accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg gaagaagcta
     1981 ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc tttggtactt
     2041 atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta agtcaaatca
     2101 acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt attttagcct
     2161 gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat ggttatcaag
     2221 tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg
     2281 atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag
     2341 tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat aatattgttt
     2401 ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg atgaagcttg
     2461 aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt cctgagaaaa
     2521 aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg
     2581 tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct ttagcttatt
     2641 actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc aacgaacggt
     2701 tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct tattctggta
     2761 attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa
     2821 ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt aaaaagccac
     2881 gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg gcttatctaa
     2941 gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac
     3001 aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga gaggagaata
     3061 aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct tcagtcaaaa
     3121 cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat tcaacaagga
     3181 tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga caattatcaa
     3241 cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt tgaaaaataa
     3301 aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt gctccctttg
     3361 cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt atcatggagg
     3421 aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt ttatcattgg
     3481 tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc aggggacttt
     3541 gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg
     3601 gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt gtgtgttaga
     3661 gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DANJJW010000177         3678 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000177 DANJJW010000000
VERSION     DANJJW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(150..1337)
                     /locus_tag="PWS82_002788"
     CDS             complement(150..1337)
                     /locus_tag="PWS82_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL0907840.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="PWS82_002789"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="PWS82_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="PWS82_002790"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="PWS82_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0907841.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..3472
                     /locus_tag="PWS82_002791"
     CDS             3305..3472
                     /locus_tag="PWS82_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907842.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            3526..>3678
                     /locus_tag="PWS82_002792"
     CDS             3526..>3678
                     /locus_tag="PWS82_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0907843.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT         1051 a          684 c          735 g         1208 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgcttttac tggttctgtt gcaaagtttt aaatctacta
     3481 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa
     3541 ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct tcgttataac
     3601 cttagctatc gtgaagttcg agaaatctta tatgatcgtg gcattaacgt ttctcatacg
     3661 acgatttatc gttgggtg
//
LOCUS       DANJJW010000178         3293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000178 DANJJW010000000
VERSION     DANJJW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWS82_002793"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWS82_002793"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL0907844.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="PWS82_002794"
     CDS             complement(2260..2511)
                     /locus_tag="PWS82_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL0907845.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="PWS82_002795"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="PWS82_002795"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL0907846.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          898 a          725 c          614 g         1056 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta att
//
LOCUS       DANJJW010000179         3213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000179 DANJJW010000000
VERSION     DANJJW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..443)
                     /locus_tag="PWS82_002796"
     CDS             complement(<1..443)
                     /locus_tag="PWS82_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0907847.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="PWS82_002797"
     CDS             513..863
                     /locus_tag="PWS82_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907848.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="PWS82_002798"
     CDS             1277..2095
                     /locus_tag="PWS82_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0907849.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="PWS82_002799"
     CDS             complement(2168..2527)
                     /locus_tag="PWS82_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0907850.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1059 a          475 c          574 g         1105 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     3181 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DANJJW010000180         3199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000180 DANJJW010000000
VERSION     DANJJW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            301..585
                     /locus_tag="PWS82_002800"
     CDS             301..585
                     /locus_tag="PWS82_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907851.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PWS82_002801"
     CDS             588..1004
                     /locus_tag="PWS82_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907852.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PWS82_002802"
     CDS             complement(1141..1860)
                     /locus_tag="PWS82_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907853.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="PWS82_002803"
     CDS             2513..2608
                     /locus_tag="PWS82_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0907854.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="PWS82_002804"
     CDS             complement(2910..3155)
                     /locus_tag="PWS82_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907855.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          498 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attacaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DANJJW010000181         3128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000181 DANJJW010000000
VERSION     DANJJW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            103..1347
                     /locus_tag="PWS82_002805"
                     /pseudo
     CDS             103..1347
                     /locus_tag="PWS82_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="PWS82_002806"
     CDS             1360..1992
                     /locus_tag="PWS82_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0907856.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="PWS82_002807"
     CDS             2197..2424
                     /locus_tag="PWS82_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL0907857.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="PWS82_002808"
     CDS             2493..2759
                     /locus_tag="PWS82_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0907858.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="PWS82_002809"
     CDS             2749..3018
                     /locus_tag="PWS82_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0907859.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DANJJW010000182         3079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000182 DANJJW010000000
VERSION     DANJJW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(299..913)
                     /locus_tag="PWS82_002810"
     CDS             complement(299..913)
                     /locus_tag="PWS82_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0907860.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1519..1896)
                     /locus_tag="PWS82_002811"
     CDS             complement(1519..1896)
                     /locus_tag="PWS82_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907861.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2196..3077)
                     /gene="galU"
                     /locus_tag="PWS82_002812"
     CDS             complement(2196..3077)
                     /gene="galU"
                     /locus_tag="PWS82_002812"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0907862.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          933 a          643 c          475 g         1028 t
ORIGIN      
        1 gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt
       61 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
      121 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      181 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      241 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      301 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      361 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      421 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      481 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      541 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      601 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      661 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      721 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      781 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
      841 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
      901 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
      961 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
     1021 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1081 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1141 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1201 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1261 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1321 tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt
     1381 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
     1441 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
     1501 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
     1561 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
     1621 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
     1681 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
     1741 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
     1801 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
     1861 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
     1921 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
     1981 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
     2041 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
     2101 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
     2161 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
     2221 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
     2281 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
     2341 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     2401 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     2461 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     2521 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     2581 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     2641 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     2701 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     2761 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     2821 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     2881 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     2941 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     3001 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     3061 gcttttttga ccttcactt
//
LOCUS       DANJJW010000183         3058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000183 DANJJW010000000
VERSION     DANJJW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1..354)
                     /locus_tag="PWS82_002813"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="PWS82_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="PWS82_002814"
     CDS             complement(614..1162)
                     /locus_tag="PWS82_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907863.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="PWS82_002815"
     CDS             complement(1163..2020)
                     /locus_tag="PWS82_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0907864.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="PWS82_002816"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="PWS82_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="PWS82_002817"
     CDS             complement(2582..2911)
                     /locus_tag="PWS82_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0907865.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          896 a          620 c          384 g         1158 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaa
//
LOCUS       DANJJW010000184         3030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000184 DANJJW010000000
VERSION     DANJJW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            202..1077
                     /locus_tag="PWS82_002818"
     CDS             202..1077
                     /locus_tag="PWS82_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907866.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="PWS82_002819"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="PWS82_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="PWS82_002820"
     CDS             complement(1642..2973)
                     /locus_tag="PWS82_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0907867.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1068 a          550 c          466 g          946 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat
//
LOCUS       DANJJW010000185         3013 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000185 DANJJW010000000
VERSION     DANJJW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            177..359
                     /locus_tag="PWS82_002821"
     CDS             177..359
                     /locus_tag="PWS82_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0907868.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="PWS82_002822"
     CDS             462..746
                     /locus_tag="PWS82_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907869.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="PWS82_002823"
     CDS             762..998
                     /locus_tag="PWS82_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907870.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="PWS82_002824"
     CDS             1020..1274
                     /locus_tag="PWS82_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907871.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="PWS82_002825"
     CDS             1356..1541
                     /locus_tag="PWS82_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL0907872.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="PWS82_002826"
     CDS             1608..2003
                     /locus_tag="PWS82_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0907873.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="PWS82_002827"
     CDS             1993..2370
                     /locus_tag="PWS82_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907874.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="PWS82_002828"
     CDS             2392..2766
                     /locus_tag="PWS82_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907875.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DANJJW010000186         2973 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000186 DANJJW010000000
VERSION     DANJJW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..113
                     /locus_tag="PWS82_002829"
     CDS             <1..113
                     /locus_tag="PWS82_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0907876.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(184..1494)
                     /locus_tag="PWS82_002830"
     CDS             complement(184..1494)
                     /locus_tag="PWS82_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0907877.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1506..2924)
                     /locus_tag="PWS82_002831"
     CDS             complement(1506..2924)
                     /locus_tag="PWS82_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0907878.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          943 a          620 c          461 g          949 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 tatatcatta aaaaaaataa aggtcaagct aaaatattat ttaagcttga ccttattata
      181 atttcaacga ttgtcaaaag tattgtttgc agctgtgttg ccagtttcta tagataaatc
      241 acagcttttg aaacataagt tttgtactgc ttcgattcgc ccgaattctt ttgcagttat
      301 ttggcaatga gaaaagcaga catcttctat aggcttttca ggaaatgcta ctaaatcaaa
      361 agctcgggaa ggaagtgagt aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat
      421 gtctttgact ttcgtcattt gctttgattc aggtatttct tcagccactg cttgccaact
      481 ttctggcatt tgagcaaggt cattgctatt ttttctattg tattcattat gccagtccat
      541 tatccaagaa aaaggaaatt gtacatcaat catattcaga ttttctaccc tgatattttc
      601 gatcactccc cctctttctt ttgaagattt cattcgaata ccacagtctg actgaaaaaa
      661 attgatatcg tgaatatata cgtcactaac tccagcacta acttcgctac caatagttac
      721 accgtatcct gaacggattt tacatctagc aacctcgatt cttgatgatt ttcgattgac
      781 tcttgcacca ttgccatctc tgccagactt aatagcaata caatcatcac cacaggccaa
      841 atcacattca tagacacgca catttgttga agaatcaata tcaatcccat ctgtacttgg
      901 tccatcattg ttgcggatga ttactttttc tactaataca tcgttagaat aagtgatttg
      961 caagttccaa aaacctgatt tctgaaaaat caaatcacga acaacaacat gttctgcatg
     1021 atacagtaaa caagcgcgtg gacgtttaat agcataatcc gcaatccaac gaagtccctt
     1081 ttgatcgtat tcagctcttg tcccgctttt ttgatctttt ccccaataca gctcccacca
     1141 atggtctcct tgaccgtcga ttattccttt tccttctata agaatatctt tcgccgagag
     1201 aacattcaaa atcgctgctg gccatttcat ttctacgccg gctactcgtg tatcgatttc
     1261 tggaaatgct tcgatatctg ttctcccctt gaggactgca ccttcttcaa agataagatg
     1321 actcttatct tttaaaaaaa gggctccgac taaataaatg ccttttggaa taaatacagg
     1381 taagtgttct ttcgcacctt gatcgattgc tttttgcagt attattgtat catcttgttg
     1441 agtgtcgttt gttttaaagt tcaaagcatt gataatccct ttttttgtcc gcaatacgtt
     1501 tctccctatc tttcattgat ttttttcaat tcttcaccaa taaatacagt ttttgaacga
     1561 ttcagtgggt aacctactag caaaatgatt gcggatacaa ataagcagaa tgctggtaac
     1621 aagtttgcca tcgtataaat cctttcttgt actaaaactg attggatggc tccacctgtt
     1681 gtagacgatt ggtaaccaat gagtgccagc ataaatccgc ctaccgctcc tgcacatgct
     1741 tgtcctactt ttctcgcaaa agaatttact ccatatactg ttccatcttc tcttaagcct
     1801 gtcttatatt gatgataatc aatcacgtca gtaatgaatg cccagaccat caaattgaat
     1861 aaaccagatc ctaaagtagc gataaataaa agaatcagat atacccatgc atttgttata
     1921 tgaaggaaat acatcagcaa gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct
     1981 tcctttttac caaataattt tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt
     2041 ccgtaagtaa ataaaagagc aacagacatt gcttgtttat tatggaagta atcattgaat
     2101 aaataagtca tattcgtccc agctaaaatc tgattgatca caatgaaaat gtcaacaatg
     2161 actaaaataa tcagtgctcg attagctaac aaacctttta ccaactgaga agcagatact
     2221 ttttcgtttt tgacaactct tactctttca gtagtcaatt tacaagttaa catatatgcg
     2281 acaaatgcta ttgcagcaca tccgattgaa atccaaaaga aatgattccc tgaaacgact
     2341 tgttgtccac tcttgttatg ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat
     2401 ccgccaatcg cagaaccaat gcttcggaac gtggacaacg aggtgcggtc attaggattt
     2461 gagctaattg ctgctgccat tgctccatac gggatattga ttgttgagag aaaaatccca
     2521 taaacaatgt atgaaacaaa aatataagca atcttcaccg tcattggaag atgttggacg
     2581 aatggcaaaa acaaaatgac agtcactaag caaaatggat acttcatttt tctgatccaa
     2641 ggaataaatc ggccagtttc tgttaactct gagacatcac ataatcttcc aaccatcaca
     2701 tcagcaaacg catccaaaat tctcgaaatc aagaataacg tacctacgat tgcccctgaa
     2761 acgccaagaa cattcgtata ataaatcatc aagaaactat tgactaaacc taaaataaaa
     2821 cagttaccta gatcaccgaa catgtagcct atcttatcgg aaatcccaaa ttcctttttt
     2881 tcttttttta ctgtatttgc tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc
     2941 tcttctagta aacgttttca aaattataac tct
//
LOCUS       DANJJW010000187         2827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000187 DANJJW010000000
VERSION     DANJJW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            229..573
                     /locus_tag="PWS82_002832"
     CDS             229..573
                     /locus_tag="PWS82_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL0907879.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            574..993
                     /locus_tag="PWS82_002833"
     CDS             574..993
                     /locus_tag="PWS82_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL0907880.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1085..1435)
                     /locus_tag="PWS82_002834"
     CDS             complement(1085..1435)
                     /locus_tag="PWS82_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907881.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1401..2621)
                     /locus_tag="PWS82_002835"
     CDS             complement(1401..2621)
                     /locus_tag="PWS82_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL0907882.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1047 a          507 c          544 g          729 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtaaaata
       61 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
      121 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
      181 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
      241 caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga attgaatgat
      301 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
      361 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
      421 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
      481 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
      541 gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga agaaatacac
      601 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
      661 caaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
      721 atttttgaac caggttgtcg caatcactgg catgtccata aagacggcta tcaaatctta
      781 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
      841 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
      901 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
      961 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
     1021 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
     1081 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
     1141 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
     1201 gtcggggatt acctaatagt tcttgatagt agatttccat actagggtcc cgaggattgt
     1261 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
     1321 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
     1381 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
     1441 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
     1501 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
     1561 tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc gtaaaaaccg
     1621 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
     1681 tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg aaaactgttt
     1741 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
     1801 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagcct
     1861 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
     1921 gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata gaaaccaatt
     1981 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
     2041 aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
     2101 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
     2161 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
     2221 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
     2281 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
     2341 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
     2401 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
     2461 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
     2521 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
     2581 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
     2641 cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt ttttgaggat
     2701 gaatataaat aacgtcagtt tcgttttcca ttacacacct tctttttcat tttctttaac
     2761 tataacttga aatgaaagat ttggacaact atcatttagg tcaaaactaa aaatgtaagg
     2821 gaaccca
//
LOCUS       DANJJW010000188         2787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000188 DANJJW010000000
VERSION     DANJJW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            387..575
                     /locus_tag="PWS82_002836"
     CDS             387..575
                     /locus_tag="PWS82_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL0907883.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="PWS82_002837"
     CDS             653..1666
                     /locus_tag="PWS82_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0907884.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="PWS82_002838"
     CDS             complement(1733..2767)
                     /locus_tag="PWS82_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0907885.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          860 a          535 c          545 g          847 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgggg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaa
//
LOCUS       DANJJW010000189         2739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000189 DANJJW010000000
VERSION     DANJJW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="PWS82_002839"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="PWS82_002839"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL0907886.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DANJJW010000190         2526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000190 DANJJW010000000
VERSION     DANJJW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1350
                     /locus_tag="PWS82_002840"
     CDS             <1..1350
                     /locus_tag="PWS82_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0907887.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2526
                     /locus_tag="PWS82_002841"
     CDS             1484..>2526
                     /locus_tag="PWS82_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0907888.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTAR"
BASE COUNT          990 a          355 c          503 g          678 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacg
//
LOCUS       DANJJW010000191         2523 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000191 DANJJW010000000
VERSION     DANJJW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..369)
                     /locus_tag="PWS82_002842"
     CDS             complement(<1..369)
                     /locus_tag="PWS82_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL0907889.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDS"
     gene            588..1958
                     /locus_tag="PWS82_002843"
     CDS             588..1958
                     /locus_tag="PWS82_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDL0907890.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1979..2263
                     /locus_tag="PWS82_002844"
     CDS             1979..2263
                     /locus_tag="PWS82_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907891.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          855 a          379 c          519 g          770 t
ORIGIN      
        1 actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa agtatcgaat
       61 aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc tactaggaaa
      121 gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt ggctagtata
      181 gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt gcttcagttg
      241 atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata tgttagaaac
      301 agttctcata atttccttga atgttataaa agtatctgta taatgttcag tatattttga
      361 tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg cgttttttgt
      421 tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc accacgcgta
      481 ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa agtatcaatt
      541 ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg atggataaat
      601 ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa gaagtctttg
      661 ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata actggggagt
      721 attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa gagcgcgatg
      781 tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat tttgcgagac
      841 tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc caaagtatgg
      901 aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat catgaccaat
      961 ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat cagcttagga
     1021 gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca tatttgaaaa
     1081 gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt gtaatttatg
     1141 ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag gcattgaaat
     1201 ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta cttgaaaaaa
     1261 tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt gaacacatca
     1321 tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg gaagaataca
     1381 caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat aattcggccg
     1441 cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt catattcgta
     1501 tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct gccagaagtg
     1561 aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg ggcgactatg
     1621 aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca actgggtatt
     1681 tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct aagattaaag
     1741 atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat ggaatcattg
     1801 aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct gtttcgaatg
     1861 cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa tatgataacg
     1921 gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg gtgttaggtt
     1981 ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa agaatccttt
     2041 tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta aatttctttc
     2101 gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata tctgggggag
     2161 tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta aggcgtatgc
     2221 attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt ttttaaacgg
     2281 agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa agaaaaatga
     2341 caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc accgacaacg
     2401 tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat ttaaatgatt
     2461 tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt cgattttcac
     2521 ata
//
LOCUS       DANJJW010000192         2505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000192 DANJJW010000000
VERSION     DANJJW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            39..656
                     /locus_tag="PWS82_002845"
     CDS             39..656
                     /locus_tag="PWS82_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907892.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1676..>2505
                     /locus_tag="PWS82_002846"
     CDS             1676..>2505
                     /locus_tag="PWS82_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0907893.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          914 a          334 c          403 g          854 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DANJJW010000193         2456 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000193 DANJJW010000000
VERSION     DANJJW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(31..744)
                     /locus_tag="PWS82_002847"
     CDS             complement(31..744)
                     /locus_tag="PWS82_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0907894.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            919..2343
                     /locus_tag="PWS82_002848"
     CDS             919..2343
                     /locus_tag="PWS82_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0907895.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
BASE COUNT          820 a          443 c          413 g          780 t
ORIGIN      
        1 ataagctctg aaaaaataat tttcccgagc ttatttttca acaaaaacag cttcaaaaac
       61 aaagtcttga tataaatgtc tcgataccga atactcgaaa ggtttaccag acgatagagc
      121 aacaactttg tctatctcaa tcataaattc acctggatct atttttaata tttttgcgtc
      181 aaattcattc gcagtatctc ctttgatacg tatgtgcgaa gtctttattt ccagacccag
      241 ttcatttgtg atataagcat atacagattt ctttaaatgt tttggttcca agcctggtat
      301 gactgctaat ggcatgaatg tttgctcaat cgagtatggt tgacaatcaa taaaacggct
      361 tcgtacaata ttatatacat agtcttttac actgatactt aatttatcag ctactttttt
      421 cgatggaagt tcaattgaga aagtatggat ttctgaatct acgtgttctt caccaaccac
      481 gctaaacaaa ccttcgtttg gaccaagaac tcgtgagtta tcaattggtg ttctgacgac
      541 ttcagtccca tacccacttc tacttcgcaa aactccttcg gaaacaagta agtctacgcc
      601 tttctttact gttaacttgc ttgtatgata aataactgcc aattcatctc ctgtaggaag
      661 tttctcacca attttatact ctccatttac aatttttttc tttatatcta agtaaatttc
      721 catatacttc gcaatttttt tcaatggtga ccctccaatc aaaaacatat cttcatctat
      781 actttaacat aaataaaccg ataaaatcca catcaaaatt atacacacaa taaaaaagat
      841 atatatattt aaacaaaact atttacttta agttgtaagc ggtgttatca ttaagtggaa
      901 acaggaggta aaaaaattat gacaactatg aaaaagctaa cttttccaga tgatttttgg
      961 tggggttccg cttggtcagc tgagcaagca gaaggaaaag gagaaacagg aaaagcagag
     1021 actatctggg atcgctggtt taaagaacaa ccttaccggt tttatcaacg aatcgattcc
     1081 tctattacaa ccgatcacat ccatcaatat aaagaagata tagaactaat gaaaaaaact
     1141 gggcataact cctttcgcgt ttctatttct tgggcacgaa tgtttccaaa tggcggtacc
     1201 ggaaaagtaa atcccaaagc catttctttc tatcgaaaat tattttccga aatgaataat
     1261 aacggaatca aagtatttgc caacttatat cattttgaca tgcctgctac tcttcaagat
     1321 aaaggaggat gggaatcacg tgaagtagtt gatgcttatg ttcattacgc tgaaacttgt
     1381 tttaaagaat ttggtgactt agtttgtcat tggtttacct tcaatgaacc acttgggcct
     1441 attttaggta cttatcttga agattttcat tatccaaacc tcattgactt taaacgcgga
     1501 gcgcaagcag cattcttcac tatacttgca cacgcaaaag caatacaagt atttaaagaa
     1561 ctagctcttc ctagtaaaat aggcgttatt ctaaatttga gtcctacttt tcctcgtagt
     1621 caaaatcctg ctgatgtgaa agcagcagaa atcgctgatt tattttatac gcggagcttt
     1681 ttagatccta tggttaaagg gaatttttct aaaaagctta tcctgttatt acaagaatat
     1741 aatcagcttc ctgaagatta tacgaaagaa gatcttgtat gtatctacga aaataccgct
     1801 caaattcttg gactaaacta ttacgagcca cgtcgcgtca aatctcgttc ttctatggtg
     1861 gatacatcta gtccattttt accagagtgg tttttcgaac cacatatcat gcctggacga
     1921 cgaatgaacg aataccgggg atgggaaatt tacgaaaaag gaatttatga tttgtgcatg
     1981 gatatcaaga accattatgg aaacatcgaa gcatttatct cggagaacgg aatgggcgtt
     2041 gcggatgaag aacgtttcat ggatgataca gggcaaataa ttgatgatta tcggattgag
     2101 tatatcaaag atcatcttgc ttacttatgg aaggcaataa atgaaggatg taacattaag
     2161 gggtatcatc tttggacatt tattgattgc tggtcatgga tcaacgccta taaaaaccga
     2221 tatgggctgg tttcactcga ccttactact caaaaaagaa caattaaaaa aagtggcgaa
     2281 ttttacaagc aattaagtga tacaaatgga tttagatacg atgtagactt gttagttgaa
     2341 taatattcta aaattccact tgaagtgaac acattagaaa acaagaataa tgagaaatac
     2401 caataacaaa tactttttct tcatacttca tttatacttg tcagtataag agactg
//
LOCUS       DANJJW010000194         2428 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000194 DANJJW010000000
VERSION     DANJJW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            128..2281
                     /locus_tag="PWS82_002849"
     CDS             128..2281
                     /locus_tag="PWS82_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL0907896.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          426 c          537 g          634 t
ORIGIN      
        1 tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct ccgcccattt
       61 tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat aggcggaagg
      121 ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat
      181 aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg
      241 cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg
      301 ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat
      361 tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt
      421 tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga
      481 tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag atgaaaatct
      541 gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac
      601 aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt
      661 caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat
      721 ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt
      781 tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac
      841 tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag
      901 tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg
      961 ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct
     1021 gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca
     1081 aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt
     1141 ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa
     1201 agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct
     1261 agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg
     1321 cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg
     1381 ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca
     1441 aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt
     1501 caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg
     1561 actagactat acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca
     1621 gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc
     1681 gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa
     1741 tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac
     1801 atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt
     1861 acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt
     1921 gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc
     1981 gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa
     2041 accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc
     2101 ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga
     2161 caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat
     2221 aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata
     2281 aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata
     2341 gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt
     2401 ttactcggca aaaaaggttg tgacaaaa
//
LOCUS       DANJJW010000195         2407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000195 DANJJW010000000
VERSION     DANJJW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            5..1150
                     /locus_tag="PWS82_002850"
     CDS             5..1150
                     /locus_tag="PWS82_002850"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL0907897.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="PWS82_002851"
     CDS             1321..1779
                     /locus_tag="PWS82_002851"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL0907898.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="PWS82_002852"
     CDS             1938..>2407
                     /locus_tag="PWS82_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL0907899.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DANJJW010000196         2353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000196 DANJJW010000000
VERSION     DANJJW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1028
                     /locus_tag="PWS82_002853"
     CDS             <1..1028
                     /locus_tag="PWS82_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907900.1"
                     /translation="NTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSF
                     DDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYR
                     NNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYT
                     GRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELV
                     CKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTIN
                     SGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIA
                     ESLDDIL"
     gene            1025..>2353
                     /locus_tag="PWS82_002854"
     CDS             1025..>2353
                     /locus_tag="PWS82_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0907901.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMII"
BASE COUNT          844 a          272 c          413 g          824 t
ORIGIN      
        1 aaaatacaat tttttccgat gagtatgttg aaattattat ttctgatcat tctaaaaaca
       61 ctaaagaaat tgcgaaaaaa atagaagaaa aaaaagtgtt tgatggctgt cattatttag
      121 aaagcaaatc ttttgatgat aatattgggt attttcaaaa gattaaggac tgtcttcaga
      181 tttcttgtgg taaatcaaat cgatatgcct attatttgaa aaactgtaaa agtgaatatt
      241 ttgatgaatt tatttgtttt tcaatgggag aggtttggtt ggaaggaata tatgcttgct
      301 tatatcgaaa taataaaaat gtacaggtat caattttaga agaaggtatt ttaagctatg
      361 gagttgacat tattccaaca gcaagaacaa aagctatcgc agtactcaat tacttaaaaa
      421 aaaggactcc actatataac aaaagctata atttttattg tttttttcct aatatatata
      481 caggaagaga gaatacaata caaattccat tagttacagc tgggtcaaga accgcattgc
      541 aactttcgga tatttttaac cctatgatag atatttataa agaaaaaaaa tatatatttt
      601 ttacgtctgt ttatgatttt gagggcggag aacctgtcgg agaatatgaa ttagtatgca
      661 aaatagcaga cttagtagga aaagataatc taattgttaa gacgcatcct agagatgtaa
      721 gaacaattta taaagataat ggatttatcg aggataaaaa ttcatcaata ccttgggagg
      781 cgattcaatt gtcaggcgat tttaacgata aaatatttat gacaattaat tcaggaagtg
      841 ttatttcagg tagtacaatg tctgcaaaac ctgtacgcac gtattatatg tataaattgt
      901 gtaacataga aggaaatccg tcttgcaaaa agaatgcgta tgatattgag cgattgctta
      961 agcaaaaaac gatgaaaaac attttaaaga atgtgaaaat agcagaaagc ttagatgata
     1021 tactatgagc aaaactaaat atttaattaa aaatatggga atattaacaa taagtaattt
     1081 ttcttcaaaa attttagttt ttttgttagt ccctttatat acaagtgtat tatccacaac
     1141 agaatatggc acatatgatt tgacaatttc aacagccaca ttattgtttc caattttaac
     1201 cttaaacatt gtagatgctg tgatgcgctt tttaatggat aaagataaaa ataaaaaaaa
     1261 tatagcatct atagcgatta aagttatatt tttaagcgtg atagtctttg gatccgtaat
     1321 gtatttgtta aatattacaa aattatggca agatgtttat ggattagaaa aatatatttt
     1381 tttgtattat atatcctatg tatggaatca atttctaata caatttgcta aagggttaga
     1441 aaaagttaaa gctatgggca tagcaggtat tatcagcaca attgtaacaa taattgccaa
     1501 tattttcttc ttgcttattc tgaaatgggg attaattggt tttttcattg caaatatttt
     1561 atcacagatt atttcagcta cttatctgtc agtattagtg agaatatggg aatatatcga
     1621 ttttgaggct aaagatcctc agctaaagag agaaatgcta aattactcga ttccattaat
     1681 tgcaactgtt gttggatggt ggattaatag tacagctgat aggtatgtgg tttcctttat
     1741 agtaggtgtc tcggcgaatg gcttattgtc tgtggcatat aaaatacctc aaataattaa
     1801 tactttgcag ggaatattta tacaggcctg gcaaatttct gcaattaaag aatatggaga
     1861 taatgagact gcaaaatttt atggaaatac atttgttatg attaatttgt taatgtgcat
     1921 aacctgttca agtcttatca tattaactag accgttttca catattcttt atgcgaaaga
     1981 cttttatgaa gcgtggaaat atgttccatt tttattaata gctaatgttt taaattgtgc
     2041 atcaggtctg ttaggaccta tcttatcagc aaaaaaagat tctaaatcca tgatgtggtc
     2101 tgcaatcata ggggcattag ttaatgtgat tatgagtgta gtccttgttt ataagatagg
     2161 ggttcaaggt gccacaatag ccactgttat atgttcatat gtaatatatg ttgttcgtaa
     2221 acgagctgtg ggaaaagata tttttataaa gcattatcat attgttgtgg aatcttgggg
     2281 attattgttt gttcaggcaa tcatagaaat atattttaaa aattatgtaa tagaaattat
     2341 aattatgatt att
//
LOCUS       DANJJW010000197         2348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000197 DANJJW010000000
VERSION     DANJJW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            155..2017
                     /locus_tag="PWS82_002855"
     CDS             155..2017
                     /locus_tag="PWS82_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0907902.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2017..>2348
                     /locus_tag="PWS82_002856"
     CDS             2017..>2348
                     /locus_tag="PWS82_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0907903.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          843 a          349 c          459 g          697 t
ORIGIN      
        1 aacgcttttt aacagtacat gtagtcgtct ttataataga taaactctgt aatattagtt
       61 aaaaagctat aagttgcact tagttaagtg caaacatttc atattgtcgt tagtttagaa
      121 aacgcttata ttagcaatgt aaggagagat gataatgcag cagctaacaa aaagagaaag
      181 gcagcttttg atttacttgg tggaaatgtc tgaaccggta acaatcaaaa agttggctga
      241 aaaagaaaat gttagtgttc ggacgataaa atacgatcta gatgatataa gagagtggct
      301 gaatgagcgg aagcaagtcc tatatagcaa acgtagtcag ggaatttgga tgaatattag
      361 tagctctgat cgaatcaaat taaaaagcga attgatggat gttaatcgtt ttgaaatatt
      421 tgcagatcaa agtctccgaa tcaaacgttt actaattatg cttttagtaa caaaagaagc
      481 tattactgga atgaaaatgg ccaacgcatt agatgtcagc aaagatacga tcatgaatga
      541 tttagatgtg ctggaaaata attttcttaa ggaaggattg accttgaatc gtcaggcgcg
      601 aaagggtttt tggatcatag gagaagaacg tctagttcga ttaacgattg aagagattct
      661 tcaaaaagag ttcacggatt atgacattta taaattaatg tcattgttat tgaatggcgg
      721 tgagactgaa tactttgaaa tgtattctgc tactgctaca cctatccaag aggtatttaa
      781 tcaagtaatc attcgaatgc gacacttact ggaatttgaa aatctagaga aactgaatta
      841 cgcggaatta ctaaatattc tgattcgagt gacaattgct accgttcgct tacgcaaaga
      901 ggctacgatt ggtcgttatc aattggttgc tgaacaagaa ttagacaaaa acgatctttc
      961 ttatcgcgtc atgcagcagg tctttgatta ttatcagttg ccactgttcg aggatgaata
     1021 tcattatatc tatagtgata cattcgacac gattcaccag caagacgtca tgaaactagt
     1081 tgagaatgtg atttataaag taagtaaagc aatcaaccat tctttttatc aagatccaca
     1141 attgttgacg aatctgtatg ctcatttatc catgcgatta tcgagaaaga aaaaatttgt
     1201 caatgaatat aatcctttta aagaagatat caagcagaaa tacccggaat tatttgatgc
     1261 gatcaagcaa gcagttcgac aagaaataag tgaacaaaat attctgatca atgattcttt
     1321 cgtggcatat attgccttac actttttggt ttcctacgaa agagaagaag atctacgaag
     1381 cgttagagcc gtctacattt gttcaacagg gttgggtgtg actagtctga tcaagcaaaa
     1441 gatcaatgaa gaattaaata atatcactat cgtggcattt gcttcagtat tgaatgcgca
     1501 agaaatgatt gctaccaaag atcctgattt ggtgattagt atttttccaa ttaatggcat
     1561 cgatcgtcct ttcatcaaag tccatccatt acccactgaa caggacttga aaaaaattca
     1621 acaaataata aaaaaaatac ttgcttcttc caatcagcag gaaaggcata acttgaatct
     1681 tagagaaacg tccatcaata aagatacttt agaagacttt agcaaggaat tgattttgaa
     1741 agcttatacg atttatgaac gtttacttgt cattttggaa gcagaattga tccaagaata
     1801 tcgagaagct tttttgcttc atgtattctt aatggttcat cgtatcacat ttgatcaaca
     1861 atatatggca gaaggtcgtc aagtagacca gattatttta gacaaaggaa cactggtagg
     1921 aaaaatcgaa gcagtttttg cagaaaataa tttaggaatc aatcagtcag aaatcagtgc
     1981 attattctca tatataaaag aaggagcgtc aagttagtga aaatgtcaga cgaggcttta
     2041 aaaattatcg aggaaagtca aaatcgggaa aagttaaaac aagtgattga tctaattgaa
     2101 gagcgattag tagaacatgc tattgtacca accgagttgc agtggaccat tctaattaat
     2161 cacttgaatg agatgctgaa acgttctcaa agaaaggaaa cgattcccgt agtcgatcgg
     2221 gatttgttca aagaagtatc caaagaagcc ataactattt ctgatgaaat agttagaagt
     2281 atcggtaatt taaccgatga tgaaatatat gttttgtcga ttcattttga aacagcaaaa
     2341 aataatta
//
LOCUS       DANJJW010000198         2254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000198 DANJJW010000000
VERSION     DANJJW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            123..689
                     /locus_tag="PWS82_002857"
     CDS             123..689
                     /locus_tag="PWS82_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0907904.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            834..921
                     /locus_tag="PWS82_002858"
     tRNA            834..921
                     /locus_tag="PWS82_002858"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ser,seq:cga)
     gene            1255..1821
                     /locus_tag="PWS82_002859"
                     /pseudo
     CDS             1255..1821
                     /locus_tag="PWS82_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            1934..>2254
                     /locus_tag="PWS82_002860"
     CDS             1934..>2254
                     /locus_tag="PWS82_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0907905.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT          806 a          351 c          423 g          674 t
ORIGIN      
        1 agttaaagaa gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa
       61 aatgctaaaa atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt
      121 taatgagaag aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg
      181 caaaggaagg gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga
      241 cacagccgat ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt
      301 ctatctttga acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca
      361 ttgatctcgg tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat
      421 tgtacattga tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc
      481 gtgaaatagt taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc
      541 tgcatactgg tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca
      601 tgcatccaac agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag
      661 cattagataa tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt
      721 ccgttggctg caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat
      781 ctattagaaa taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt
      841 tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag
      901 gttcaaatcc tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca
      961 tcaagtaata agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct
     1021 tattaccgca gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga
     1081 cttttgtcac agccccttat tgttgtactt ttaacctaca atattttttg attccccctg
     1141 agtactattt tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa
     1201 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt
     1261 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
     1321 tcttataatg aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta
     1381 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
     1441 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
     1501 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
     1561 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
     1621 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
     1681 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
     1741 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
     1801 aattatcgct cgctatcaaa tggaacgaga gtataaaata ttttgtgtaa atgaaaaaat
     1861 ccatacaaaa aaggaagtcc cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa
     1921 agaggacttc cctatgaatg attttactac agaaattgtg caaactctag tcactaaagg
     1981 cgatttaaat gaattattcc gttcgcactt agaaaaagcg ataaacacac tcctacggac
     2041 tgaattaacg gcttttttag attacgaaaa atatgatcgc actggtttta attcaggtaa
     2101 ttcgagaaac ggttcttact ttcgatcaat caaaaccgaa tatggtgaat taacattgga
     2161 aatacctaga gatcgtaatg gtgagtttaa acaacaaact ttaccagcct acaaaagaac
     2221 aaacgataca ttggaaacca ctattatcca ttta
//
LOCUS       DANJJW010000199         2222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000199 DANJJW010000000
VERSION     DANJJW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..544
                     /locus_tag="PWS82_002861"
     CDS             <1..544
                     /locus_tag="PWS82_002861"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0907906.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="PWS82_002862"
     CDS             complement(900..1472)
                     /locus_tag="PWS82_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907907.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="PWS82_002863"
     CDS             complement(1488..2093)
                     /locus_tag="PWS82_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL0907908.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          698 a          451 c          336 g          737 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tt
//
LOCUS       DANJJW010000200         2202 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000200 DANJJW010000000
VERSION     DANJJW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..914)
                     /locus_tag="PWS82_002864"
     CDS             complement(<1..914)
                     /locus_tag="PWS82_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL0907909.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWS82_002865"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWS82_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL0907910.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="PWS82_002866"
     CDS             2006..2176
                     /locus_tag="PWS82_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907911.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          744 a          384 c          422 g          652 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gt
//
LOCUS       DANJJW010000201         2179 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000201 DANJJW010000000
VERSION     DANJJW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(14..235)
                     /locus_tag="PWS82_002867"
     CDS             complement(14..235)
                     /locus_tag="PWS82_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907912.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(333..578)
                     /locus_tag="PWS82_002868"
     CDS             complement(333..578)
                     /locus_tag="PWS82_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907913.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            818..1432
                     /locus_tag="PWS82_002869"
                     /pseudo
     CDS             818..1432
                     /locus_tag="PWS82_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1802..>2179)
                     /locus_tag="PWS82_002870"
     CDS             complement(1802..>2179)
                     /locus_tag="PWS82_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL0907914.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          744 a          378 c          308 g          749 t
ORIGIN      
        1 aaagagcgaa ccattataga tcagctcttt tttcattgct gtttttaatc ttatctagaa
       61 tcacttcaaa tgtttcaaag gtatttacat ctaactgaca taaccaaagc aagtacttct
      121 tttctttgta agtccaatca atattacggt aagaggacat tagattatgg taccttgctc
      181 tttgttcaac tgaagccaat tcccagcgtt tttcaaatat tttttcttct tccattattg
      241 atccctcgca agacgctacc tcaaacttaa ataatgggca gcgaaagtct aattccttag
      301 atctaagtat agcaaacaaa tatgaaaaaa acttagaagg tagtaaaagt tcttccgtct
      361 tttgctttgc ttctattctt aatgtgtttc gttatcggtg ccctacaaag attttcatta
      421 attacacact catttattgt tgtgtatttt ctgacatttc tctcttgatc aattatttta
      481 attccatggg catcctgata gttcatagga gaggagcggc tatcagaaat agttgcacct
      541 atctcttgaa cttcttttac aacttctgga aaatccatcc atcctgcttc agctgtcaaa
      601 agtaataatc tataatattt atctttcctt ttcaatctaa gaaatcgtca ccccattctt
      661 tttcttctat tataaactat ttaactttta gaattcaaaa accgtttgtc tatttagagt
      721 ttaccctaaa tagacaaaaa tataacattg aaaaatttag agttctaacg tatgattagg
      781 gtgtattata aacgttatag aaaagtaggg aataaatttg aaaattgctt atattagagt
      841 ttcatccatc gatcaaaatg aacaacgtca aattgaagaa atgaaaaagt ttggcgctga
      901 aaaaattttt atcgaaaaac aatctggagc gacgattagt catcggccga ttttccagga
      961 agctttagat tttgtcagag agcaagatat ttttattgta gaagccattg atcgacttgg
     1021 acgcaattat gacgagatta tcgattcagt gaattatcta aagaagaaga atgtgcgact
     1081 tattatcacc agtttaccga tcatggctga agcaattgga aatcctctcc tagataaatt
     1141 catcaaagac ttaatcattt aaatctcagc catgattgct gaacaagaac gtactgaatc
     1201 gaaacgacgc caggcacaag gaattaaaat cgctaaagca aacggcgtct ataaaggtcg
     1261 tcctaagtta tacagtgcag acaccaaaga tcctcaacgt cgccttgtct ataggagtat
     1321 cgttcaggat cttgagaatg gggttgctat ctcaaagatt gcaacagact acaatgtgac
     1381 taggcaaaca atttaccgaa taaaaaaaga aatcgaccaa ctaattgtat gatttctttt
     1441 ttgaatcacg taattattaa ttagctttgt catcaataca gacaatcatt ttagattcat
     1501 ctagctaagt tgattacatt tatgttaaaa agaatttagt taacataaat ataattgatg
     1561 cataaatcgt ccttatttaa aggcatttaa cattgacaac ttagtataat gtatcttatg
     1621 taaactaata agatttgaat tttattttat acacactcga taatagatca attttatggt
     1681 cccccgcatt tacgattgca caagagaaac ataaagtcga cctattagca tttcttttca
     1741 attcaaacat taaaaaagtt tatacctctg ctactaaaaa atgaaattat ctatttaaaa
     1801 tttaaaacgg taaatctgat tcatccaaat tggaaaatgg atcagcctgt attggagctt
     1861 tgtatttctc cataataaca gatatctttt tttcattttt taaaaaatac cttttaaatt
     1921 tcttatagtc ctctgaaatc aaaatatagc gtgatggata ctcatcaaaa gaagaatcgt
     1981 attcttcctt taaaattccc gatttactta atttttctag tacatcaaat actctatcac
     2041 tcaagtaact ccttaaattg ttatcacttt cccattgaac tacttttcta taagttgaat
     2101 tatcagttga aaagatagaa ttttcatcca taaacgcata tattagcagc aatctttcat
     2161 cattgtcaaa ttctattgg
//
LOCUS       DANJJW010000202         2158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000202 DANJJW010000000
VERSION     DANJJW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            17..>2158
                     /gene="acm"
                     /locus_tag="PWS82_002871"
     CDS             17..>2158
                     /gene="acm"
                     /locus_tag="PWS82_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL0907915.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIF"
BASE COUNT          846 a          347 c          422 g          543 t
ORIGIN      
        1 aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat
       61 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
      121 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      181 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      241 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      301 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      361 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      421 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      481 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      541 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      601 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      661 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      721 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa
      781 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
      841 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
      901 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
      961 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
     1021 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1081 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1141 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1201 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1261 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1321 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1381 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1441 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1501 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1561 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1621 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1681 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1741 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     1801 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     1861 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     1921 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     1981 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     2041 tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa
     2101 atctagtttt gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattc
//
LOCUS       DANJJW010000203         2144 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000203 DANJJW010000000
VERSION     DANJJW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            116..370
                     /locus_tag="PWS82_002872"
                     /pseudo
     CDS             116..370
                     /locus_tag="PWS82_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            440..952
                     /locus_tag="PWS82_002873"
     CDS             440..952
                     /locus_tag="PWS82_002873"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0907916.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            949..1986
                     /locus_tag="PWS82_002874"
     CDS             949..1986
                     /locus_tag="PWS82_002874"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0907917.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          773 a          398 c          439 g          534 t
ORIGIN      
        1 tgtttcttaa ttgtatatct tgatattaaa cgatttaaga ttattcttca agatatatat
       61 tcgcaaatgt aattggaaaa gcgacttctt aaacaaaatt aaggagtcgc tttttatggc
      121 aaaaataacg aaaaatgagc aagaaaattt ggtggaaaat tacacaaaaa aaccggcgta
      181 ctctaatagc cggttagacg cacatttcgt gtgcacttct aatccgaaat tgagctttga
      241 cgctatgacg atagtcggaa accttaataa aaacagcgct aaaaagctat ccgattttat
      301 gagtttagat ccacaaatta gactttggga tatacttcaa acaaaattta aagctaaagc
      361 actacaagac cccctatgtt aggctagttg tcgtatagga taataagtta taataaagac
      421 tatacggagg atgattacca tgacaaataa aaacaatgaa cgttactcta ccgaactgaa
      481 agcgacagcc atcaagaaaa tgatgccacc cgaaaatagg gggattcgcc aattaagtca
      541 aactttgggc attccagaag cgactcttta ttcttggcgt aaaaaagcta gaattcaagg
      601 gaatactaca cccggaacga aaaaaaatgt cgaaaaatgg tcaagtgaag ataaattttt
      661 aattgtgatg gaatcattct caatgaacca agtagaactt gcggaatatt gccgaaagaa
      721 gggcttattt aaagaacaaa ttgaggcttg gaagaaaacg tgcttatctg cgaatgaaca
      781 ggaagaaaat cgaacgagag aacttgcaac tgaactaaaa gaagagaaaa aacaagcgcg
      841 tcaattagag aaagaattac gtcataagga aaaagcatta gcggaagccg ctgctctctt
      901 attactaaga aaaaaagcac acgcgatttg gggggaccaa gaggaagaat gatttcatct
      961 tcagatcgca aacaagctgt tgaacttatt caagaagcca atcgcaatgg ggcccgtctc
     1021 acctatgcct gtaatgagct gaatattagc gttcgaacct acgaacgatg gacgcgtgaa
     1081 ggtacgatag cgcatgatca acgccctttg gcgaaacgtc ctgtacccaa aaataaactg
     1141 acggatcaag aacgcgaaaa aataatagaa accgtttcaa aaaaagaatt tatgaatttg
     1201 ccacctagcc aaattgtacc aaaacttgcg gattgctcga tttatattgc atcagagtct
     1261 agcttctatc gtgtattgcg agaaaaaaat atgcaacatc accgtggccg aagtcaagtt
     1321 tcacaaaaac gcataccgcc aagtcacctt gcgacgaagc caaatgaagt ttggacttgg
     1381 gatattactt ggctaaaagg tccaataaaa ggactctttt accggttata tctcatcatt
     1441 gatttattta gccgtaaaat ggtaggctgg gagatttggg aatcagaaga agctcgtcat
     1501 gccgaaaaac ttattaaaca gacggtgctt aaggaacgta tccaaggaca gccgttggtt
     1561 ttacactccg ataatggcag ccctatgaaa gccgcgactt ttcaagtctt actcgaaaaa
     1621 ttaggaattc aaagttctta ttcaagacca cgagtgagta atgacaatcc ttattcggaa
     1681 gcggctttcc gaacgttaaa atatcgacca gaatatccca ataacggctt taaaacaata
     1741 gatgatgctc gaaaatggac gaaagagttt gtcgattggt ataacgattc tcatttgcat
     1801 agtgggattc aatttgtgtc tcctgcagcg tgtcatgaag gacgacatct cgctatttta
     1861 gagaaacgaa agcaggttta cgaggacgct aaaaagcgcc atcctgaacg ctggtcaaaa
     1921 ggtgttcgaa attggactcc acatcaatcg gtggcgttga atcctttaaa agaaacagaa
     1981 agttaacagg agcataatct ttcaagatta tcaaataacc ctcgtcaccc aataggggaa
     2041 aaagttattc ttcccctatt ggataacgag gccaaccgac catagggagg ttctgctttt
     2101 tgataggtgg aaaataaaaa tacgacaact atattgacaa acac
//
LOCUS       DANJJW010000204         2140 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000204 DANJJW010000000
VERSION     DANJJW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            1..237
                     /locus_tag="PWS82_002875"
                     /pseudo
     CDS             1..237
                     /locus_tag="PWS82_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347575.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            210..1502
                     /locus_tag="PWS82_002876"
     CDS             210..1502
                     /locus_tag="PWS82_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0907918.1"
                     /translation="MFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFLIFS
                     LIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENISILK
                     FDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFVIV
                     FMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSL
                     NFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDI
                     NMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKL
                     GITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYIL
                     NTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            1495..>2140
                     /locus_tag="PWS82_002877"
     CDS             1495..>2140
                     /locus_tag="PWS82_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL0907919.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYR"
BASE COUNT          822 a          227 c          350 g          741 t
ORIGIN      
        1 agaatgagtt atcacagaat attgtgcgaa tagtaacgat tcttatattt cagttatttt
       61 tagtcttaat taaagaagtg tatggatata taaaaactat ttatgcattt gaagttgatt
      121 tatcaatgaa aattgatttt cttaaggttt tacaaaaaaa aaatcttttt ttttcgcaaa
      181 cgaagcattc aggagaaatt caatttagga tgttttcgga tatttctact atcgtaaata
      241 ctatctttat tttttctgtt gaattacctg tgataggcgt tttagttctt gtagcggtat
      301 ttattataag taaagtgaat cttatttttc tgattttttc actaataatg atgggagcta
      361 tggtgttaca gtataattta tttcaattac cgcttaagaa aacaatcgag aatacaaaaa
      421 gtgttaatca aagtattaca ggagaattga atgcacattt tgatcaatta gaaattataa
      481 gcgcatataa ttttgagaat atctctattt taaaattcga ttctagattt aaagaattat
      541 ttaatgtttt caaaaaacaa aataaattac taacactggg aagctgtttg gcacaggtta
      601 ttttccaatt atggattgga ggaatatttc ttattgatgt tattttatta gctaaaggaa
      661 caataagtat tactaatttt gtaatagtat ttatgtacgc taacatggtc cctactttat
      721 ggactacttt aattagtatt atttggaaat ggcaagacta ttcagtgtca ttaaatagat
      781 attacgaata ttattcaaat aacaacaaag tggctggcac aaaaaaaata aataaaataa
      841 attctattac tggtcaatca ttaaattttt catataacaa aagaaaggta ttaagcaata
      901 taagtttttc aactgaaatg aataaactat tcatgatatc aggagaaaat ggaacaggga
      961 aaacaacact gatgaaatta tttactaaac agttgccggt tagtaataat caattattcg
     1021 tcaacgggat agatattaat atgattgata acgaatcatt aagaaggtta atttcaatcg
     1081 caacacaaat accatatata attaatagct ccgtgaagga gaatatttta atggggacta
     1141 aaaactatga taaaaaaaaa ttcagtaaat taatagaact attgggaata agtaaattta
     1201 tgacgaaatt aggtattact ttggacgaca atatttatga tgaaaaaata ttattatctg
     1261 gaggagaaaa acaaaaaata agtttagcta gggcgcttat taaatcagct tcagttatta
     1321 tattagatga accatttaga aatttagata atgaaactcg cgataatttg agggattata
     1381 tactaaatac aaaaaaagaa gtttgttata taattataac tcatgatcac attctagata
     1441 attttttgga ttcaaatgat gaaatattat atttaaataa ggagtatgat aataatgaat
     1501 agcaaaaaac ttcagatggg tgaaattgtt agaggtccta gtcaaatatc tattgatgtt
     1561 acatataaat gtaattttag atgtaaacat tgttacaatg cgagtggtgg agacaattgt
     1621 tttatggata atgagttgac tgatgatgaa ctgttagaac ttacaaaaga tatatgtaat
     1681 ttaaagccat ataatatttg tttctgtgga ggagaaactt tactgagaaa aaaaatagtt
     1741 ttatcttgtg ccactttatt aaaagaggca ggtattccta atattgctat tgttactaat
     1801 ggatcattag taactcagga tgttgcaaaa gaattattca aagcaggtat taatcgattt
     1861 caagtaagtt tagatggaaa aaatgctagt agccatgaag cacttagatt atatccaaac
     1921 agttttgaaa aggcaattaa tgctatcaaa ttatttagag aattgtccga agaagtggat
     1981 gttgcttttt gtcctactag ttttaattac aatcaattac gagaagtata tcaactagta
     2041 aatactctta aagtgaagtc tttaagaatt caaccattta tgcttagtgg taggggaatg
     2101 caaaataggg aaatagtgcc aactgtttct caatatagaa
//
LOCUS       DANJJW010000205         2122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000205 DANJJW010000000
VERSION     DANJJW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            7..525
                     /locus_tag="PWS82_002878"
     CDS             7..525
                     /locus_tag="PWS82_002878"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0907920.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            653..1336
                     /locus_tag="PWS82_002879"
     CDS             653..1336
                     /locus_tag="PWS82_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL0907921.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1645..1718
                     /locus_tag="PWS82_002880"
     tRNA            1645..1718
                     /locus_tag="PWS82_002880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Arg,seq:tct)
     gene            1877..>2122
                     /locus_tag="PWS82_002881"
     CDS             1877..>2122
                     /locus_tag="PWS82_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL0907922.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSM"
BASE COUNT          717 a          333 c          430 g          642 t
ORIGIN      
        1 aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt
       61 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
      121 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      181 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      241 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      301 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      361 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      421 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      481 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      541 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      601 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      661 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      721 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      781 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      841 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      901 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      961 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
     1021 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1081 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1141 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1201 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1261 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1321 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1381 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1441 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1501 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1561 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1621 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1681 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1741 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1801 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1861 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1921 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1981 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     2041 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2101 atctaaagaa atcacaagta tg
//
LOCUS       DANJJW010000206         2122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000206 DANJJW010000000
VERSION     DANJJW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(652..942)
                     /locus_tag="PWS82_002882"
     CDS             complement(652..942)
                     /locus_tag="PWS82_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907923.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1150..1842)
                     /locus_tag="PWS82_002883"
     CDS             complement(1150..1842)
                     /locus_tag="PWS82_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0907924.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          605 a          393 c          312 g          812 t
ORIGIN      
        1 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
       61 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
      121 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      181 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      241 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      301 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      361 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      421 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      481 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      541 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      601 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      661 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      721 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      781 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      841 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      901 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      961 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
     1021 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1081 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1141 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1201 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1261 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt
     1321 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
     1381 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
     1441 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
     1501 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
     1561 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
     1621 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt
     1681 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
     1741 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
     1801 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
     1861 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
     1921 acatgtgtaa ttttaatata aatactatca aatactcatt taatcctcct tttttatcta
     1981 tttaaaaaat cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta
     2041 tggtgaacaa aaacaaaaaa gcaagtttta ttaaacgtaa cccttttcat ttcatagatt
     2101 actgtattta ttacaaaaaa cg
//
LOCUS       DANJJW010000207         2059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000207 DANJJW010000000
VERSION     DANJJW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..338)
                     /locus_tag="PWS82_002884"
     CDS             complement(<1..338)
                     /locus_tag="PWS82_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0907925.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(405..560)
                     /locus_tag="PWS82_002885"
     CDS             complement(405..560)
                     /locus_tag="PWS82_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0907926.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1177..1398
                     /locus_tag="PWS82_002886"
     CDS             1177..1398
                     /locus_tag="PWS82_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907927.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1399..1656
                     /locus_tag="PWS82_002887"
     CDS             1399..1656
                     /locus_tag="PWS82_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907928.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1792..>2059)
                     /locus_tag="PWS82_002888"
     CDS             complement(1792..>2059)
                     /locus_tag="PWS82_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0907929.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          463 c          314 g          604 t
ORIGIN      
        1 cttttagcat attgaaattt ttctggatta tccatatgag caaaatatcg caccatacct
       61 tttgcattag cacatttttg cgggtttgga gaccgaagct tttgagttat ctcacgtatt
      121 tgatcatacg tctttttacc gctaaacatt atcaaaacat gccaatgagg ctttttcaat
      181 tcaccgtcag gattcacatc tttatcatga agtggactct caacccaagg aacatgtaac
      241 tcatctaaaa actccctcca attttcagga gccgattcag gatagactac aaacgtccaa
      301 ttccttgtgc gttcatcttt agtttttcta ctcgtcatgc tataattacc ttaattgaac
      361 aacagtctaa cagcaaaagc agtgcgccaa cactaatgct tttgctattt tttttgctca
      421 ttttcctttc cgttagcatt aacccaaatt gtaataagac cagacttact aagacccatt
      481 tgtttcgcaa ttttatcaag attatctgcc acttccttag taagcgttac aacgactctc
      541 tttttttcat tactgatcat aattcgtatc ctccaacgac tttttcttaa aaaccatagt
      601 aacaaaaaag taactaaggt gcaacagttt agatactaat tagcgaataa taggccttaa
      661 acccttggca ttactgattt tgtcaaaaaa aaacgctgaa acccttggga gagtaaggct
      721 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      781 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      841 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      901 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      961 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
     1021 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1081 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1141 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1201 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1261 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1321 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1381 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1441 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1501 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1561 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1621 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1681 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1741 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1801 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1861 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1921 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1981 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     2041 atgagcgata atatcactt
//
LOCUS       DANJJW010000208         1991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000208 DANJJW010000000
VERSION     DANJJW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="PWS82_002889"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="PWS82_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0907930.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="PWS82_002890"
     CDS             852..1040
                     /locus_tag="PWS82_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0907931.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="PWS82_002891"
     CDS             1053..1610
                     /locus_tag="PWS82_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0907932.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="PWS82_002892"
     CDS             1642..1884
                     /locus_tag="PWS82_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0907933.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DANJJW010000209         1991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000209 DANJJW010000000
VERSION     DANJJW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            604..936
                     /locus_tag="PWS82_002893"
     CDS             604..936
                     /locus_tag="PWS82_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL0907934.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1555..1896
                     /locus_tag="PWS82_002894"
     CDS             1555..1896
                     /locus_tag="PWS82_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL0907935.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          689 a          268 c          421 g          613 t
ORIGIN      
        1 actccttttt tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag
       61 ctaaaaatct tgatattact tcttttcagg caatacgaat attattcgct tacatttttt
      121 gtataaattt aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga
      181 tgataaattt ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct
      241 ggtaaccttc gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt
      301 tcagtgttac ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca
      361 ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc
      421 ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg
      481 gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct
      541 atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat
      601 tttatgtcat atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt
      661 accgctacag agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag
      721 tgtaagaaat atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg
      781 aaacaaaagc aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc
      841 aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt
      901 tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat
      961 tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac
     1021 tagaaaaata aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag
     1081 gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag
     1141 tggtgaaatg aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt
     1201 agacaagtta gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg
     1261 acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt
     1321 gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga
     1381 gcatttaccg ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt
     1441 ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa
     1501 aaaattaata taaagcatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa
     1561 aattatgatc cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg
     1621 gattacaaag gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta
     1681 gcattagacg aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc
     1741 ttgctacctg aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat
     1801 ggagacgaat tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt
     1861 gaaatcattg aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag
     1921 ataaaggctt agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga
     1981 gtaaggaatt t
//
LOCUS       DANJJW010000210         1978 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000210 DANJJW010000000
VERSION     DANJJW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..838
                     /locus_tag="PWS82_002895"
     CDS             <1..838
                     /locus_tag="PWS82_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907936.1"
                     /translation="NQFFSDDLSVYDEIFIQGENYFTKILFSNVYKKNPDVKLNYIED
                     GLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKI
                     DENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKET
                     LIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTI
                     NSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYG
                     II"
     gene            825..>1978
                     /locus_tag="PWS82_002896"
     CDS             825..>1978
                     /locus_tag="PWS82_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0907937.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          675 a          247 c          310 g          746 t
ORIGIN      
        1 taatcaattt ttctcggatg atttatccgt ttatgatgaa atttttatac agggggaaaa
       61 ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa aatccagatg taaaactaaa
      121 ttatatagaa gatggtttgg gtacttattt gaagaagagt atattttcaa caagtaaaaa
      181 cgttcaaaga gtttttaaaa tattaaataa aaatagcatc ttcaaaaaaa aaattgacga
      241 ttattttgtc tattgtccgg aattgttact ttatgatgta aaaaatataa aaaaaattcc
      301 caaaatagat gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga
      361 agtttttgtc ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg
      421 catagatgaa acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac
      481 tttaattaag cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa
      541 ttccagttta ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat
      601 tagtcctata tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc
      661 tattatgttg gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga
      721 aacattgaca aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat
      781 tttactccca gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca
      841 cactttccaa gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta
      901 ttaagggaat ttcattcata actattccta tttttgtaaa tattactact caagcagaat
      961 atggtttgat taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa
     1021 gcctaaattc ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta
     1081 tgtcatcggt actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca
     1141 tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt
     1201 tattacaaag ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact
     1261 ttaaccattt taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct
     1321 ctgtagcttt gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg
     1381 gaacactgtc atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg
     1441 ctcatttttt tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc
     1501 cacattcctt agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt
     1561 taggtcctgc tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg
     1621 taatatggat gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa
     1681 ataatgaaaa aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta
     1741 cgatgattgt aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt
     1801 attctgggtt atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata
     1861 gtttaccagt taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag
     1921 tagcaagtgc tatagttaat atactattaa acatgatttt catacctagg ttaggaat
//
LOCUS       DANJJW010000211         1968 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000211 DANJJW010000000
VERSION     DANJJW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..176
                     /locus_tag="PWS82_002897"
     CDS             <1..176
                     /locus_tag="PWS82_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0907938.1"
                     /translation="LNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDL
                     YHANEVLKIAEEK"
     gene            177..929
                     /locus_tag="PWS82_002898"
     CDS             177..929
                     /locus_tag="PWS82_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0907939.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            934..1881
                     /locus_tag="PWS82_002899"
     CDS             934..1881
                     /locus_tag="PWS82_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL0907940.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          691 a          263 c          390 g          624 t
ORIGIN      
        1 tattaaataa taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta
       61 atgatatgta tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa
      121 actggaatga tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg
      181 aaattttatt tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga
      241 aatatttggc aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac
      301 aaaaacatcc tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag
      361 aattgacgac taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta
      421 cagatactgt agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag
      481 ataatatatt tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta
      541 atcttgaaga attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag
      601 ttgtttctgc aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta
      661 ctatagttaa taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga
      721 atgcctcaat gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg
      781 ggcaatgtgg gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag
      841 attttaaatg gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag
      901 atatctataa ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg
      961 gctggcttca ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc
     1021 gtcgatgact tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt
     1081 attgagggag atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt
     1141 gattatatct ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta
     1201 gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat
     1261 caacctgatt taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg
     1321 actttaccaa aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa
     1381 ttcgctgcag aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca
     1441 gttcgttttt tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg
     1501 atttctattc tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca
     1561 ttatttggtg atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca
     1621 ttacttttag ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt
     1681 aaagcaacca cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg
     1741 acacttgaat acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact
     1801 aagatccagt caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat
     1861 ttgaaaactg aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct
     1921 ttgtcaataa ggactgatga gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DANJJW010000212         1928 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000212 DANJJW010000000
VERSION     DANJJW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            142..756
                     /locus_tag="PWS82_002900"
     CDS             142..756
                     /locus_tag="PWS82_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907941.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(921..1745)
                     /locus_tag="PWS82_002901"
     CDS             complement(921..1745)
                     /locus_tag="PWS82_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907942.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          640 a          324 c          286 g          678 t
ORIGIN      
        1 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
       61 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
      121 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
      181 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
      241 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
      301 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
      361 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
      421 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
      481 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
      541 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
      601 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
      661 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
      721 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
      781 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
      841 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
      901 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
      961 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     1021 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     1081 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     1141 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     1201 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     1261 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     1321 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     1381 ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt
     1441 tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt
     1501 ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt
     1561 tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac
     1621 tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga agtataattt
     1681 gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca
     1741 atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa aaaacattta
     1801 tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat
     1861 ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt
     1921 ccattact
//
LOCUS       DANJJW010000213         1901 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000213 DANJJW010000000
VERSION     DANJJW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..117
                     /locus_tag="PWS82_002902"
     CDS             <1..117
                     /locus_tag="PWS82_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0907943.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            172..1407
                     /gene="aph(2'')-I"
                     /locus_tag="PWS82_002903"
                     /pseudo
     CDS             172..1407
                     /gene="aph(2'')-I"
                     /locus_tag="PWS82_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1391..1783
                     /locus_tag="PWS82_002904"
     CDS             1391..1783
                     /locus_tag="PWS82_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0907944.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          769 a          163 c          332 g          637 t
ORIGIN      
        1 acgattaaag gcatggaagc catccgagga ttatataaga aaacccgaaa agaaggcact
       61 ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc
      121 atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac cgaatcatta
      181 aaaaaacatt atacagagcc ttgggaagat gaagttttta gagtaattat tgaatataac
      241 aatgttccta ttggatatgg acaaatatat aaaatgtatg atgagttata tactgattat
      301 cattatccaa aaactgatga gatagtctat ggtatggatc aatttatagg agagccaaat
      361 tattggagta aaggaattgg tacaagatat attaaattga tttttgaatt tttgaaaaaa
      421 gaaagaaatg ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg
      481 gcataccaaa aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag
      541 ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt
      601 aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga tagtattgaa
      661 ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata catttttaaa
      721 acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt
      781 ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt
      841 gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt
      901 tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta
      961 agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga taataaacaa
     1021 aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata
     1081 gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt
     1141 aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat
     1201 agattaactg gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt
     1261 atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta
     1321 agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa
     1381 tattatccta ttgaaactat tgtttattga tgataatatg gtttttgata ttgataattt
     1441 aaaaggattt cttaatgata ccagttcatt tgggtttata gctaaagaaa ataataaaat
     1501 tataggattt gcatattgct atacactttt aagacctgat ggaaaaacaa tgttttattt
     1561 acactcaata ggaatgttac ctaactatca agacaaaggt tatggttcaa aattattatc
     1621 ttttattaag gaatattcta aagagattgg ttgttctgaa atgtttttaa taactgataa
     1681 aggtaatcct agagcttgcc atgtatatga aaaattaggt ggtaaaaatg attataaaga
     1741 tgaaatagta tatgtatatg attatgaaaa aggtgataaa taaatgaata tagttgaaaa
     1801 tgaaatatgt ataagaactt taatagatga tgattttcct ttgatgttaa aatggttaac
     1861 tgatgaaaga gtattagaat tttatgatgg tagagataaa a
//
LOCUS       DANJJW010000214         1897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000214 DANJJW010000000
VERSION     DANJJW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..652
                     /locus_tag="PWS82_002905"
     CDS             <1..652
                     /locus_tag="PWS82_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL0907945.1"
                     /translation="GMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH
                     DRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(738..1832)
                     /locus_tag="PWS82_002906"
     CDS             complement(738..1832)
                     /locus_tag="PWS82_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0907946.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          593 a          389 c          305 g          610 t
ORIGIN      
        1 cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg acacactacc
       61 gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca
      121 gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct
      181 tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag
      241 attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac
      301 tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga
      361 ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata ttgatatata
      421 ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct
      481 gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc
      541 cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat
      601 cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat
      661 aacctataaa aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt
      721 ctgcaataac taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc
      781 aaaaagtcaa ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg
      841 tcgtttttca tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca
      901 ttgtcaatca gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta
      961 tctttgtagt atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga
     1021 taactatgaa ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta
     1081 tctactttca atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct
     1141 tcacttgcgt acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc
     1201 ttcacttgtg tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc
     1261 gtaggccaag ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta
     1321 tattttctga atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga
     1381 tgaggataaa aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg
     1441 ttgtcaacgt aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg
     1501 cgcgtcattt ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca
     1561 cccatcttat ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct
     1621 ggattgatat caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta
     1681 aattctgaaa gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt
     1741 ttatacccat acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt
     1801 tctggataaa tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt
     1861 ttttgtatcc acttacatta tagcaaacac aaagtaa
//
LOCUS       DANJJW010000215         1816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000215 DANJJW010000000
VERSION     DANJJW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1208)
                     /locus_tag="PWS82_002907"
     CDS             complement(<1..1208)
                     /locus_tag="PWS82_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL0907947.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICI"
     gene            complement(1201..>1816)
                     /locus_tag="PWS82_002908"
     CDS             complement(1201..>1816)
                     /locus_tag="PWS82_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0907948.1"
                     /translation="EFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          665 a          292 c          216 g          643 t
ORIGIN      
        1 tagatacata taaaagcaat tataccactt gatacaagta cttgaagaaa tccattatgc
       61 atgtttccat gtgctctttt tagttcattc acatctgtag tatcaagatc tacttttttc
      121 aaaaattttg catcgctatt cccatagaaa tcggtctctc cataaccaaa taacaaagtt
      181 ggtcttataa ctttaagcgc agctttccaa atcgtaaaac gaccagctga aatttcggag
      241 ttatcactag aatgctgtaa tattacttct ttttctggaa gtggagtttt atctgattca
      301 tttgactcta taacttctac ttttctatca atcgatacag gaattgttgc taaaaaatcc
      361 ttagcataat tagtaccaaa agaaaaatat accaatagaa ctaataatat gcttaccatc
      421 catttgattt ttacaggtag tacttctcta atgctctttc taaattttga attggcaaac
      481 aacaaaagaa ataatactaa aaaagaacaa attactacct gggcacctct agacgaagaa
      541 agtgtaatat aaaatatttg tacgataaaa ttcatcatat aaaaaacacg aatcgttttt
      601 gttatttttg aaattaatac actaattaaa cttaaaataa tagaagtata tccaaacata
      661 gctccaacat ttggacttgt atagacacca aataaccgat gttcaataaa accttgtctt
      721 gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa tcaaaaaaat aaattgatag
      781 atagatatta gcgcagctaa aaaaattatg ataatgaaaa cattattaaa atacaccatt
      841 tttttctttt tttcctcttc actttgtgaa aagtctgcag gaaaaattac tatcaaagta
      901 atcagcaaat atccaaaatt ataaatagat ggaattaaaa aattttctct gttaatgatt
      961 atagttaaga taaaactaat cattgctcca atcagtagcc aagaaaaacg cattttaaaa
     1021 aaatttttct ttataaaaac ctcatacaaa actataattc caccccaagc aaaagctatt
     1081 ttcatcggga tattagttac attaaaagta ctaggcataa aataaagcag acaaatgatt
     1141 aaatttatta ctcgaaacgc atctcgactt aataaactct tctctaaaac aggtactatt
     1201 ctattcatct atatttccaa ctttcaaaat ttctttatca attctattta atagtttatc
     1261 agagtaatcc gatttaactt ttcttacaaa taatttttca ctttctttta tttgatctat
     1321 atccctttca tcaaaccatt tgttcatgga aggatcaata atatgaatat ttgctaattt
     1381 cgaagtttgt aaagtagttt cttcaatatt tttcaattca agtggtaaat ctggtcctcc
     1441 aataacattt ttatttttga acgaggaatt aaaaaaaagt gtatgaaaaa atttttcatc
     1501 tggggcaaac atacctattt taaaattcag gtagtcttgt tgattttgtt catacatttg
     1561 tattaattct gttaggcatt ctttcgacaa agcccaccat tgagaaccat gatacaaatc
     1621 aaatttatgg tgtctaattt ttgggggaag tttaatcatc ctcagacaac gattaattat
     1681 agctctaaaa attttaaatg cagttgtgtt attcctatta attctaggat aatcatgttt
     1741 ttgatataca tctatatgtt tataataaaa ttctttatgt ttaatttgat ctagatctat
     1801 tccacgaata aactcc
//
LOCUS       DANJJW010000216         1807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000216 DANJJW010000000
VERSION     DANJJW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            120..1046
                     /gene="cysK"
                     /locus_tag="PWS82_002909"
     CDS             120..1046
                     /gene="cysK"
                     /locus_tag="PWS82_002909"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL0907949.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1282..1728
                     /locus_tag="PWS82_002910"
     CDS             1282..1728
                     /locus_tag="PWS82_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0907950.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          615 a          272 c          402 g          518 t
ORIGIN      
        1 aattaccata tatggtatat ctttgtcttt tttttgctag tcaaagacga atcactcgcc
       61 ataaatgaac agattgtatt atgataagta agaagagcaa agacaaggag gaattgaaga
      121 tgacagctat ctataactca gtcaccgaat tgattggcaa aacgccaatc gtcaaattaa
      181 ataaaatcgt accagaagat tcagcagatg tttttgtaaa gcttgagttt tttaatccag
      241 gaggaagtgt aaaagatcgt attgctttaa gtatgataga aaaagcagaa cacgatggtc
      301 tactgaaacc aggtgatact atcattgaac ctacttctgg aaatacagga atcggattgt
      361 cgatggtagg agttgcaaaa ggatataaag taatcatcgt gatgcctgag acgatgagta
      421 tcgaaagacg tttattgatg aaagggtacg gcgcagagtt gattttgact cctggtgcgg
      481 atggtatttc tggatcaatc agagaagcag aacgtttggc aaaagaaaac ggctactttt
      541 taccattaca atttgaaaat gaagcaaatc ctcttgtgca tgagaagacg actggtcctg
      601 agatacatca agcatttgga gtaaatggct tggacgcgtt tgttgcaggt atcggtacag
      661 gaggaacgat cactggcgct ggccgagaat taaaacgtgt gtatccgaaa atcgaattaa
      721 tcggggtaga accagcagaa tctgctattt tagaagggaa agaagcggga cctcacaaaa
      781 tccaaggaat cggtacaggt tttgttccta aaacactgga tacttctgtg tatgataaag
      841 tcctttctat tagtggagac gaagcgatgg aaacagctag agaagttggg cgaaaagaag
      901 ggattttagt aggcatctca tctggcgctg caattgctgc cgcgctaaaa gtcgcaaaag
      961 aattaggaaa aggcaagaag gtattagctg tggttcccga caatggtgaa cgttatttat
     1021 ctacagccct ttaccaagag atataaggca cttggttttt ctccagatag gtttatgtaa
     1081 aaaactttta aagatgaaaa gaacacacaa aaatttatga tgagaaaaat tatttgaata
     1141 gagacgtaga atagtgaacg cgactgtttt acgtcttttt tcattaaaat acaataaaat
     1201 tgaattttct tttcatgtta agtggatttt aaaatgtaaa aatagtatga taagacgaga
     1261 ataacaacgt ggggtgttta catggaaaat aagttagtcg aagaggttat cgaaaagtta
     1321 aaaaaagaga acattcgaat cacacctcaa cggtacgcgg ttttagaata tttgattgaa
     1381 catcattccc atccaacggc tgatgagatt tataaagcac ttgaacatcg gtttccaaat
     1441 atgagtgttg cgactgttta caataacctt cgcttattta cttcgattgg atttgttcaa
     1501 gaaatgaagt acggcgatgc ttctagccga tttgatttca gttcaaagcg tcattatcat
     1561 gtaatttgtg aaaactgcgg taagattgtt gattttcatt acccagggct tgaagatata
     1621 gaaatggctg caagcagact gactgatttt gagattaatg aacatcggtt ggaattgtat
     1681 ggactctgtc cggattgtaa agagtcagca aaaaaggcgc aaatgtaaaa acagtttcta
     1741 caagtgttat acctaaaaaa agctagctgt gtaaaaaaaa cgctagcttt tttatttttt
     1801 tgtcttt
//
LOCUS       DANJJW010000217         1803 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000217 DANJJW010000000
VERSION     DANJJW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..339
                     /locus_tag="PWS82_002911"
     CDS             <1..339
                     /locus_tag="PWS82_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL0907951.1"
                     /translation="TKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQV
                     GNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDI
                     LDEYKHDILI"
     gene            395..613
                     /locus_tag="PWS82_002912"
     CDS             395..613
                     /locus_tag="PWS82_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907952.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            684..1499
                     /locus_tag="PWS82_002913"
     CDS             684..1499
                     /locus_tag="PWS82_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907953.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            1563..>1803
                     /locus_tag="PWS82_002914"
     CDS             1563..>1803
                     /locus_tag="PWS82_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL0907954.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILK"
BASE COUNT          693 a          205 c          309 g          596 t
ORIGIN      
        1 accaagatag attggggaga tccaattgat catatcatcc gacacagaac tttattgaaa
       61 gatttttcga attttgtttc tattaggtca gacggaggat taatggcatc accttacttg
      121 ccaattcaag ttggaaatat taaatgtcac agtctttcta aatattggcg agcaggactt
      181 tataaagttt tttctttaga tttagttcaa gatatagcta ctcaagtttc ttcaatacaa
      241 gatatgagta aagaattaaa aaaaggacat attaaatggc ttaatgaaga tatagaaatt
      301 gatattctcg atgaatacaa acatgatatt ttaatttagg tttatcattg gaaagtttag
      361 ataaacaaag tacttttaga aaggagggaa aaaaatgatt tctgaacctc aaatgaataa
      421 tatgtatgaa agcccaacta taatgggagc tggtgaagct gcgccaagag cttgttcagc
      481 aattatggtg tgtgtgttat tagcatctgc aggagtaggt atccaagcag gagcggtgta
      541 tacttatgct gtagttgcta cagtagctgt agctgcagcc gcagtttgga aaacagtagg
      601 attagttggt taggaattaa taggagacta actgtctcct attgattcca atgattaagc
      661 taaggaggaa ttttaagaaa ataatgaaat acaataattt aaaaccgaaa ttacttaatg
      721 agaacataga atatataaga gaagaaaatt atggatattt aaccatagtt cccaaaaatg
      781 gaactgaatt gaaagataac gttttcaatc caacagtaag atatatattg gctaactgta
      841 ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa aaagaagttt cctagtttag
      901 aatatgaaaa attctatcaa gatgttgtag gaactattaa atttttcaaa cactataata
      961 ttattgatat aggagattat agtatggaag aaaagaaaat atataaaatt gaacaaaatt
     1021 tagaattaga attactaaat gaatcacatt atgaagaaat ttataatttt atcacagatc
     1081 atcctgaaag tatgtatttt catgaagata tttatcatca aggaaatagt aaagaattag
     1141 aagttgtcct tagatataat ttattttcgt ttacgagtga attttacttt ttaagacata
     1201 aagagagaat tgtaggaatg gtttctataa ttgttaatcc atttcaaaac ttatcctaca
     1261 gtatagtaaa ttacctcata tgtgagcctg catatactac gactattttg acaacagtat
     1321 tagaaaatat taacgaagtt tcaattttag aagtaaagaa gctagtgatt catttgctaa
     1381 attcaacaga agatgaatca ctaatcccct atttaaaaga ttcacatttt tttaaagaag
     1441 cggtactatc taaagaatat agagagcatg atgttttgag atacgcttac atttattaaa
     1501 taaaatttct gatagtattt taataattcg gatcatatca taagatatag gagggaaaag
     1561 aagtgatttt attgattttg tctctcgtat tactagttgt atctaatcta atgaaaaata
     1621 atcctcctac aaaaattaat tctttttatg gctttagaac taaaaagtca atgaaaaatg
     1681 aaaaaagttg gcatttagca caaaaattgt tgggggattt ttcaaaaaaa aaagttatcc
     1741 cagtattttt gatgggtatt gtttttttca gtatagagat ttatagttta gctattttaa
     1801 aaa
//
LOCUS       DANJJW010000218         1745 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000218 DANJJW010000000
VERSION     DANJJW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..182)
                     /locus_tag="PWS82_002915"
     CDS             complement(<1..182)
                     /locus_tag="PWS82_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0907955.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKE"
     gene            complement(179..832)
                     /locus_tag="PWS82_002916"
     CDS             complement(179..832)
                     /locus_tag="PWS82_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0907956.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1120..>1745
                     /locus_tag="PWS82_002917"
     CDS             1120..>1745
                     /locus_tag="PWS82_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0907957.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          557 a          318 c          237 g          633 t
ORIGIN      
        1 atttcttttc cattcatatt actagaaatt tcatttgcca tattatgaat cttttctttt
       61 ctcgtctcca aatatgtttt aggaaagata aaaaaaatta atgaatgaac taatataatt
      121 attattccaa gaacagaaaa tatttgtatg aacatttttg gaaatatctt taattttttc
      181 attttctctc caatttatat cctacatttc ttatagtagt tatacagtct aattgtaatt
      241 tttttctaag ttcttttata tatacatcta ttactcgatc ataaggagtt tcttcgctat
      301 ctttccatac ataatcaata atttgcattc ttgttaacac ttgtccatta ttatccaata
      361 aacattttaa tacttctagt tcttttgcat ttacatctat ttcttcacca tttattttag
      421 ctgtatagct attaaaatta accgatagat ccttatattc aaacttctct aaatgtccat
      481 aattcttctt aattaaagaa tctattctag cttttaagac cggcaatgaa aatggctttt
      541 ctacatatcc gtctactaaa ttagtaaatg catcaatttt atattcttca tcactaaatg
      601 cagtcaagat tagaattggt aaattgcttt tctttctaat ttctttcaac acttctaaac
      661 catttataaa aggtatctga atgtctaaga taaccaaatt tatatcgcta ttaaattttg
      721 acaaggcttc tcttccatct ttagcttgaa taacagtata tccaaattct gaaagatatt
      781 cacttattcc ctctcttatc atattatcat cttcaacagt taatattttc atatatacct
      841 ccacttaagc ctatacacta gatattatac agcatattat ggctctataa tatctagtgt
      901 tggtgacacc ttctgttgcc aatacttttt cttttttcta ataaatgcaa acagacacct
      961 tgaaatcctt tagaataaag ggttctataa tattttgtgt tgttggaaaa tcgaaggcga
     1021 ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt
     1081 tagtcgacca aaacatcacc ctaccaaagg agtcgctcta tggataatca tactagaaaa
     1141 ttactcggat taacagataa acatttattt tttgatgaag aatggctgat tgaaaaagaa
     1201 tataaaggcg ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt
     1261 gaacactgtg gatcgattcg tattatccgc aacggttcct atacgacacg tactcagata
     1321 ttgaaagtca aagaaaagtt gactatttta gaattaaaac gtacccgttt tctatgctat
     1381 gattgtgggc agaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca
     1441 aaggaactga agcaggcgat tctcatagaa ctgtacgaaa atcaatcacg taaactaatc
     1501 gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag
     1561 cattaccagc cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca
     1621 atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac ccatcagcta
     1681 tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct
     1741 agaaa
//
LOCUS       DANJJW010000219         1653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000219 DANJJW010000000
VERSION     DANJJW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            152..1024
                     /locus_tag="PWS82_002918"
     CDS             152..1024
                     /locus_tag="PWS82_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0907958.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1320..>1653
                     /locus_tag="PWS82_002919"
     CDS             1320..>1653
                     /locus_tag="PWS82_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0907959.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT          528 a          309 c          362 g          454 t
ORIGIN      
        1 aaaattatta gaattttaaa aaatgataaa aattcaaaaa gaaaaaatag tagttattta
       61 cttgtcatgt tactaaataa aaaaataatt taaatatgat atatagtcat ttatttagtg
      121 atttcattct aaaataaagg gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg
      181 cacaaaattt gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt
      241 tccgacaacg acacctgagg aaacaatgcc tttagtcatt gacttcttca aacaatacca
      301 agcagattta gcagggattg ggattggctc atttgggcct atcgatatcc atcgcgattc
      361 cgccacttat gggtacatta cctcgacccc gaaattggca tggcaaaatt ttgatttcat
      421 cggtaccatg aaaaaagaat ttccgatccc gatttcttgg acaacagatg tcaatgcggc
      481 tgcctatggt gagtatgtct tcggcagtgg caaaggattg agcagtgtcg tttactatac
      541 gatcggtacg ggtgttggcg gcggtgcctt acaagaaggg cgctttatcg aagggtttag
      601 tcatccagaa atggggcata tgctcgtcgt ccctcatcca aaagatgact ttgctggcag
      661 ctgtccattt catggcaact gtttagaagg gatggccgct ggacccgcca tcgaaaaacg
      721 cttagggaaa aaaggacaag aggttgcaga agatgatcct tattgggaaa tcgaagcctc
      781 ctatattgct caatgtgcct acaatacaac cttgatgttt tctccagatg tgatcatttt
      841 tggcggtggt gtgatgaaac aacgccatat gctgcaaaac gtccacgacg catttgcaaa
      901 attggtcaat gggtatgtcg aaacacctga gttaagcaaa tatatcgtga ccccagcatt
      961 agaagacaac gctggaacgc tcggttgttt ggcattggct cgagaagcgg tcttacattc
     1021 ataaacgaaa aaaggctctt cgacatttag tgttggtaga gaaagataca actgaaaatt
     1081 tagtcataac cagttaggca gagtgcttaa ctggtttttg ttttacttgg aagatcaagc
     1141 cttgaatgag ataaattctt gctcgaaagt tatggaaatt tcgatagccg taggccactt
     1201 gtattgtgca atagtttttt acacgccgtt tgtataatta agcgagacaa ataaaaaagc
     1261 catttatgtt acactcaaat cgtaataccc gctaaagcat acaaggagag tgaacataaa
     1321 tgacttatac ccatcttaca ccaaacgagc ttgtaatgat agaagcatat tttcatcaag
     1381 aaactccggt tgctatcgtt gcgaagcagc ttaaacgtgg acgccaaaca atttacaatg
     1441 tctataactt tctcaaatgt ggtggaacag cacttgaatg ctttgaacaa tacaaagaaa
     1501 ataagcgacg ttgtgggaga accgaaatca tttttcctgc tgaggaaaaa gaatacattg
     1561 caaaaagatc aactgaaggt tggaccccag acgtcattat tggccgtgca gagcgaacct
     1621 tctcttgttc agtaagtacc ctctatcgcc ggt
//
LOCUS       DANJJW010000220         1644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000220 DANJJW010000000
VERSION     DANJJW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            316..570
                     /locus_tag="PWS82_002920"
     CDS             316..570
                     /locus_tag="PWS82_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0907960.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PWS82_002921"
     CDS             571..888
                     /locus_tag="PWS82_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0907961.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1644)
                     /locus_tag="PWS82_002922"
     CDS             complement(1522..>1644)
                     /locus_tag="PWS82_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL0907962.1"
                     /translation="NSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          576 a          259 c          312 g          497 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agtt
//
LOCUS       DANJJW010000221         1614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000221 DANJJW010000000
VERSION     DANJJW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            52..474
                     /locus_tag="PWS82_002923"
     CDS             52..474
                     /locus_tag="PWS82_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907963.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            484..687
                     /locus_tag="PWS82_002924"
     CDS             484..687
                     /locus_tag="PWS82_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907964.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            898..1482
                     /locus_tag="PWS82_002925"
     CDS             898..1482
                     /locus_tag="PWS82_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0907965.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          569 a          227 c          298 g          520 t
ORIGIN      
        1 aagatttatt aagaaaacgt tttcttagtt ttgatttcga ggtgatagac aatgaaaaat
       61 aagtttattt tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt
      121 cctacatttg ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta
      181 actcctttag atagggacgg aatagccatt ggtaacccaa ctaacgactt aactgttact
      241 gaaaattatt taatagtttc tccattactt gctctagcag cttctaaaac gatacgagtg
      301 acaggaacga tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat
      361 tggaaagaaa aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa
      421 tcagctggaa acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt
      481 tttatgccag aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa
      541 attaatttac cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa
      601 aaaattcaaa aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac
      661 caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc
      721 tttttttcta attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc
      781 tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc
      841 agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg
      901 aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc
      961 ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat
     1021 cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg
     1081 cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat
     1141 gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct
     1201 acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc
     1261 ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt
     1321 cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca
     1381 cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg
     1441 gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct
     1501 gaaaattttt gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc
     1561 tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat atta
//
LOCUS       DANJJW010000222         1603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000222 DANJJW010000000
VERSION     DANJJW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(62..280)
                     /locus_tag="PWS82_002926"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="PWS82_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="PWS82_002927"
     CDS             complement(546..740)
                     /locus_tag="PWS82_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907966.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="PWS82_002928"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="PWS82_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          564 a          242 c          213 g          584 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta tta
//
LOCUS       DANJJW010000223         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000223 DANJJW010000000
VERSION     DANJJW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANJJW010000224         1528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000224 DANJJW010000000
VERSION     DANJJW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            15..1481
                     /locus_tag="PWS82_002929"
     CDS             15..1481
                     /locus_tag="PWS82_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL0907967.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          497 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctgtt
//
LOCUS       DANJJW010000225         1510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000225 DANJJW010000000
VERSION     DANJJW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            102..869
                     /locus_tag="PWS82_002930"
     CDS             102..869
                     /locus_tag="PWS82_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0907968.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1061..1459
                     /locus_tag="PWS82_002931"
     CDS             1061..1459
                     /locus_tag="PWS82_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0907969.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          562 a          189 c          282 g          477 t
ORIGIN      
        1 aaatactgtt aaatatctcg aaaaatcaat tacgagttat ttgccttatg tgaaccttta
       61 tgattaacaa ctgaaaaaat gcgtgaagga aggaaaaact tttgaaaaaa gatgaactga
      121 caaagtacaa tgaggagttc ttagcaacaa ttgacatgaa aatggattta aagtcgtatg
      181 aactttatca attgctgttc agttatatct cacccacaaa gctaaagaaa acaggcgtag
      241 tagatttacc tgttaaagcg attattaatt acatggatgt tgatcaaaat cttgaaacgg
      301 aactaaaaac catagttaac actttgcaaa gacaagcagt ttatgaaatt tcgaatctag
      361 acaaaaaata taagacgtat actactgtat ctgctattga tcggattcat tgggatttag
      421 aagagcatag tgtatctttc agtttttcag aagatgtaat tcccttaatt gtctcaatca
      481 aaaagaagta tccgttcttt gtttttgaag acttagcatc ttttcaaaaa aaatatagca
      541 tcgtgttata caaatatttg gcgatgtatt ataaaaaata tcttatcgcc aaaaataaag
      601 gagattttga gaaagctgaa atatataaga atcccctgat tgaaatagtt aaaattcgtg
      661 agattacgaa aacagaaaat gagtataaaa caatatcgaa ttttgataga cgtgttttaa
      721 gggaatcagt tgaggaaatt aatgaaaaat caatgctgaa tattgatttt gctaaagtta
      781 gaaaaggcag aaattacaat tttgctgtcc agtttaatat ttctagtgag tttgatgaaa
      841 aaacttatag agaaaaaaaa ttaggttaat gcaaaatata tcttactcgt ttgaagatgc
      901 ttcagacgta tgctaagata tatagtgcct tgggagcaag gtattaatct cctttagagg
      961 ttaattagct tgtgatcgcc tatcaatttt tttggtaggc gattcttttt tttgaaacaa
     1021 aaaagcaaaa attatttttt aattagagag gtgcaatgcg atgaaatact ttttttgtgc
     1081 agtattcgaa tggaaaggaa atgaatatgc agttaaattc ccagattttc cagacttata
     1141 tacacatggt gaaactttag aagatgcttt tgagatggcg caagaagtat tggaaggata
     1201 cttggtgctt ttagagttgc aagaaaaaga aattccatcg cctacgcctg ttcaagagtt
     1261 aaatgcaaga atcaagcgat ctgagaatgt taatttattt gtcgtaaaag ctaatacgaa
     1321 agttgctaaa gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg tggaacacta
     1381 tttaaatgtt ttagcggctg aagagaatat taatctgtcg aaagctttga atgatttgtt
     1441 gagagaaaag ttgatttgat gtctctcaga tgatcatatt aagcaaaaac agcttgtttt
     1501 aggacgtatg
//
LOCUS       DANJJW010000226         1476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000226 DANJJW010000000
VERSION     DANJJW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            286..693
                     /locus_tag="PWS82_002932"
     CDS             286..693
                     /locus_tag="PWS82_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907970.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="PWS82_002933"
     CDS             707..1108
                     /locus_tag="PWS82_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907971.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..>1476
                     /locus_tag="PWS82_002934"
     CDS             1110..>1476
                     /locus_tag="PWS82_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907972.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          567 a          181 c          258 g          470 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactca
//
LOCUS       DANJJW010000227         1475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000227 DANJJW010000000
VERSION     DANJJW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..848
                     /locus_tag="PWS82_002935"
     CDS             <1..848
                     /locus_tag="PWS82_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0907973.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1362..>1475
                     /locus_tag="PWS82_002936"
     CDS             1362..>1475
                     /locus_tag="PWS82_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0907974.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          485 a          260 c          289 g          441 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttgg ttctgttgca aagttttcca aaaaatctat
     1321 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     1381 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     1441 gctatcgtga agttcaggaa ttgttatatg atcgt
//
LOCUS       DANJJW010000228         1454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000228 DANJJW010000000
VERSION     DANJJW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..625)
                     /locus_tag="PWS82_002937"
     CDS             complement(<1..625)
                     /locus_tag="PWS82_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL0907975.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="PWS82_002938"
     CDS             complement(784..1050)
                     /locus_tag="PWS82_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0907976.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="PWS82_002939"
     CDS             complement(1040..1396)
                     /locus_tag="PWS82_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0907977.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DANJJW010000229         1451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000229 DANJJW010000000
VERSION     DANJJW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            361..>1451
                     /locus_tag="PWS82_002940"
     CDS             361..>1451
                     /locus_tag="PWS82_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907978.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          488 a          140 c          258 g          565 t
ORIGIN      
        1 ttgttgagaa gatgtgcttc tagtcatgtg atgggagaaa ataatttaac tgttagagtg
       61 aatagttaaa ttattctggt tatatgaaaa aagtagtttc taaaataatc ttgcagggat
      121 taacttaaag aaattgagag aatagtttct ttgaaatttc tatatacaat gagaagctga
      181 aatttacttt tatttgatga ctggaaatag aaattaaata tattgtattg ttacattaaa
      241 gaaaacttgt atggaaaaat cagtacaata ccattattta atgttcttca cttaaagcgc
      301 aaaaaatatc agtttattaa actcgtatat agaacaaata tatgtagacg gggcgaaacg
      361 atgagtgtaa aaaaaatgct atttatggta agtacaataa ttgtattaac atcccgattt
      421 tggggaatta atactagttt atctatgcga tattttatta tggcaatatg ggatttatat
      481 tttatgttaa gctatttttc ttatcgtgag aaaacatata aaaaatacgt tatcaagaat
      541 aacttttggt tgactataat gccgtttgtg atattttcta tctatactct aataatatgg
      601 ggaattggtt caaatgcaga atttgaaaat tttataaagt tatgttccac gttgctttat
      661 ttaattcttg catatggtta cgcatgtaca gcattttact tatttaaggc agaaggaatt
      721 gattttattt tttggtcagg tgttatttca tatatgagtg gttcagtttt ttatttaatt
      781 gtatcttatg gtttaaatgg tttaatttct tatataaaaa gtcttataac aggtgaaaca
      841 aatactgcaa attttgctat ggaggtacat gatttaactt ttgcgatggg ggtttttttc
      901 ctttattacg tattttttga aaataaaaat acaaaaaaac ataagataaa aattatcgta
      961 tctttaattt taattttcat gggattgaag agaattgaac tgttagcttt aagtggtgca
     1021 attctagtgt attatgtttt attaaagtgg ggaaaaaaga ttcaaatccg tgcattagtg
     1081 tgtactattt gtgcaactgt tatttcgtta gtgtttattt acattataga tacaggaatt
     1141 ttggcattgc ttatgtcaaa tttgggcatt acagatgacg gtgcgaggct ttcattttat
     1201 ctatattcat ctaaatttta tgaacttggt ttaggatatg taggaacagg atggacatgg
     1261 ttttcaaaat acttttttaa tctttattta tcgcacttta ggttaaatgg tggaagaatt
     1321 gcagcaagcc tacacagtga tatattaact atttatatag aagttggatg tatagttttt
     1381 attatatgga tgttttggtt attttttgtg aaaccgataa gactaactaa aaaatttgga
     1441 ttgaaaacag g
//
LOCUS       DANJJW010000230         1435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000230 DANJJW010000000
VERSION     DANJJW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            105..1406
                     /locus_tag="PWS82_002941"
     CDS             105..1406
                     /locus_tag="PWS82_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0907979.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          411 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgtt
//
LOCUS       DANJJW010000231         1399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000231 DANJJW010000000
VERSION     DANJJW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(933..1346)
                     /locus_tag="PWS82_002942"
     CDS             complement(933..1346)
                     /locus_tag="PWS82_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL0907980.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          515 a          233 c          207 g          444 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataa
//
LOCUS       DANJJW010000232         1328 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000232 DANJJW010000000
VERSION     DANJJW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            143..1183
                     /locus_tag="PWS82_002943"
     CDS             143..1183
                     /locus_tag="PWS82_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0907981.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          542 a          183 c          209 g          394 t
ORIGIN      
        1 aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg
       61 cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca
      121 acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg atagagttta
      181 caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat
      241 gaaagattca actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag
      301 caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg
      361 tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt
      421 tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa
      481 tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt
      541 tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca tacccaccct
      601 cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg
      661 ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt
      721 agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt
      781 agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt
      841 attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac
      901 tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag tttttgaaga
      961 actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta
     1021 tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa
     1081 aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaatta
     1141 tgcaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt
     1201 gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct aattatatta
     1261 cgaatattac aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa
     1321 aatcacaa
//
LOCUS       DANJJW010000233         1324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000233 DANJJW010000000
VERSION     DANJJW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            278..820
                     /locus_tag="PWS82_002944"
     CDS             278..820
                     /locus_tag="PWS82_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907982.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="PWS82_002945"
     CDS             976..>1324
                     /locus_tag="PWS82_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL0907983.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DANJJW010000234         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000234 DANJJW010000000
VERSION     DANJJW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1244
                     /locus_tag="PWS82_002946"
     CDS             <1..1244
                     /locus_tag="PWS82_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL0907984.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANJJW010000235         1249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000235 DANJJW010000000
VERSION     DANJJW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            2..631
                     /locus_tag="PWS82_002947"
     CDS             2..631
                     /locus_tag="PWS82_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0907985.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(745..1119)
                     /locus_tag="PWS82_002948"
     CDS             complement(745..1119)
                     /locus_tag="PWS82_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0907986.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
BASE COUNT          424 a          225 c          175 g          425 t
ORIGIN      
        1 tatgaaaatt gtttttttgc atggtctagg ccaagacgaa caatcatgga aagatgtaat
       61 ctcttatcta cccaatagtt attcttgcaa aagtctttca ttgttcaatc atttaaacag
      121 cacagacact gctactttag acgacttaac caaatacatc gaagtacaat tgaatcagat
      181 aaaggaacct tttattttat gtggattatc tttaggcgct gttattgctt taaactatct
      241 tgcaactcct tctccatatt taaaaaaggt tgttttagtc gctccacaat ttgagtctcc
      301 taatcgctta ttgcttactt tccagaatat ttatttttac tgcgtacctt ctaagatatt
      361 tgaaaaatta ggtctatcta aaaaacaaac ccttacccta cttcgttcac ttgaaacatt
      421 agactgcaaa aaacagcttc gtacatcacg acttcctaaa ttgattttat gcggtaccaa
      481 agatcatgcg aataaaccag cagcaaaacg tttatataaa ctttcaacga atggccggct
      541 actctttatt aaaaacggaa aacatgaatt gaatagagaa aagccagaaa taatggcaca
      601 agctatgcga tcttttatcg aaaatagtta atttaaaaaa agacgacaaa atgtgtcgtc
      661 tttttattta ccatggtaag aatgttgtct tgttttttca atacagcttg attgagacta
      721 gttttatgga caatagagag aaagttattg ttttattcca gagaattttt ctggtcgtag
      781 atctactaat tgaacatgac tgtctaaaga accacttggg ctttcaagag taaatacacc
      841 tagacgtgga tctaaagaaa gggtcatatg ttcatccatg tacatttttg aataatcttt
      901 tatataaaca tccccaatat cactatctaa ttggagttta taatcatttt ttctctgttc
      961 tttatcaaga agaagtaaac gaaaactagt atctaaagag caaacaccca ttttagaata
     1021 ttccccaacg ccatcatcta aatccaatat aactgttgcg ccttcatcaa tatatttttg
     1081 taatctttct tgtgcttgtt ccgtaatttt aagatacata actaatacgc cctctttctt
     1141 tttcttacaa aagcatgcta gcatatatta ataaaattat ctaaagtcct gctcaaaaca
     1201 aaaagatgta agaaatatct tatgatattt cttacatctc agagtgtag
//
LOCUS       DANJJW010000236         1201 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000236 DANJJW010000000
VERSION     DANJJW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>1201
                     /locus_tag="PWS82_002949"
     CDS             <1..>1201
                     /locus_tag="PWS82_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL0907987.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLIN"
BASE COUNT          397 a          234 c          232 g          338 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 a
//
LOCUS       DANJJW010000237         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000237 DANJJW010000000
VERSION     DANJJW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1076
                     /locus_tag="PWS82_002950"
     CDS             <1..1076
                     /locus_tag="PWS82_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0907988.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DANJJW010000238         1183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000238 DANJJW010000000
VERSION     DANJJW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..153
                     /locus_tag="PWS82_002951"
     CDS             <1..153
                     /locus_tag="PWS82_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0907989.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="PWS82_002952"
     CDS             159..575
                     /locus_tag="PWS82_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0907990.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="PWS82_002953"
     CDS             681..944
                     /locus_tag="PWS82_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0907991.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="PWS82_002954"
     CDS             944..>1183
                     /locus_tag="PWS82_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0907992.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DANJJW010000239         1143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000239 DANJJW010000000
VERSION     DANJJW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="PWS82_002955"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="PWS82_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL0907993.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DANJJW010000240         1114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000240 DANJJW010000000
VERSION     DANJJW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(243..1091)
                     /locus_tag="PWS82_002956"
     CDS             complement(243..1091)
                     /locus_tag="PWS82_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0907994.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          352 a          190 c          194 g          378 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aata
//
LOCUS       DANJJW010000241         1114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000241 DANJJW010000000
VERSION     DANJJW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="PWS82_002957"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="PWS82_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL0907995.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DANJJW010000242         1106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000242 DANJJW010000000
VERSION     DANJJW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1087
                     /locus_tag="PWS82_002958"
     CDS             <1..1087
                     /locus_tag="PWS82_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0907996.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DANJJW010000243         1103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000243 DANJJW010000000
VERSION     DANJJW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1..634)
                     /locus_tag="PWS82_002959"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="PWS82_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          341 a          190 c          189 g          383 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tat
//
LOCUS       DANJJW010000244         1077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000244 DANJJW010000000
VERSION     DANJJW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>1077
                     /locus_tag="PWS82_002960"
     CDS             <1..>1077
                     /locus_tag="PWS82_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL0907997.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNA"
BASE COUNT          346 a          218 c          212 g          301 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaa
//
LOCUS       DANJJW010000245         1074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000245 DANJJW010000000
VERSION     DANJJW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..276)
                     /locus_tag="PWS82_002961"
     CDS             complement(<1..276)
                     /locus_tag="PWS82_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0907998.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            838..>1074
                     /locus_tag="PWS82_002962"
     rRNA            838..>1074
                     /locus_tag="PWS82_002962"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          351 a          194 c          205 g          324 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaataaac atttttaaca
      781 agcaagcaat atgctagcaa acaaattgag cttaacaatc gcaagattgt tcgaactttt
      841 tatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
      901 gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg cgaacgggtg
      961 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata
     1021 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg tgtc
//
LOCUS       DANJJW010000246         1051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000246 DANJJW010000000
VERSION     DANJJW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PWS82_002963"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PWS82_002963"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL0907999.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DANJJW010000247         1014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000247 DANJJW010000000
VERSION     DANJJW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>1014
                     /locus_tag="PWS82_002964"
     rRNA            <1..>1014
                     /locus_tag="PWS82_002964"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          272 a          229 c          310 g          203 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgt
//
LOCUS       DANJJW010000248         1002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000248 DANJJW010000000
VERSION     DANJJW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(85..999)
                     /locus_tag="PWS82_002965"
     CDS             complement(85..999)
                     /locus_tag="PWS82_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908000.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEY
                     INDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKN
                     LSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILL
                     TIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          324 a          167 c          152 g          359 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatattttt agaaaaagca
       61 gtcggacttt ctaaaagaag ttcactattc gatcctgtcg atgatttctt caggaaaatc
      121 gtctatttgc ttgtcaattt tgctgtcttt gtatttctct tcttggatag tcaacaaaat
      181 ctttgtaaga ttcccccagt ctactgctct tggcggatcg agccaaacca ctacttgaga
      241 tgcactttct tcatatagat tattcctatt cgtgattata acatccggga actcaggctt
      301 tcctgcctct atcacttccg tgttcagatt aatgtacaag cttaaatttt tcttgataaa
      361 ttgattatag gcagcaccat aagaaaaatc gatgatgatc tttactggtt cagaaatcag
      421 ttgaatgggt atgtgattga tcaataataa taaataactg aaaaaaagaa aaaatttatc
      481 ttgaagcaac tctttgcgtt ttgacgttag atcatttata tactctatca aatagcaata
      541 aaatgctgga tagttttctc taaaaaaaga aacatcaagt ggaaattcat cagctttatt
      601 aatagtgtaa tgcaaaagtt taaaatgtat tttggttaac tctcttcgga tcaattggct
      661 cgtctcttct cgcaacagat taaactgatt ttcaaaactt tcaatgaatc gttcattcaa
      721 ccattcgatt gtttcatctt cattttttct caagtatttt tgatccaatt tatcatgaag
      781 ataaagccaa gagagtgtct tattgataaa aataacggat ttttctccta atactccttt
      841 taagtggtaa aatcctatat ccgaaaaaaa tagcgactga ttgctagcaa tatctaaaga
      901 atcagcttgt tcagccaaca ggttgattgc taaaaagtgg aataatttct ttttttcata
      961 agcagacaga ggataatact gttttaaatc ttttaacatt tt
//
LOCUS       DANJJW010000249          997 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000249 DANJJW010000000
VERSION     DANJJW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(26..913)
                     /locus_tag="PWS82_002966"
     CDS             complement(26..913)
                     /locus_tag="PWS82_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0908001.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DANJJW010000250          986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000250 DANJJW010000000
VERSION     DANJJW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..704
                     /locus_tag="PWS82_002967"
     CDS             <1..704
                     /locus_tag="PWS82_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0908002.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DANJJW010000251          959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000251 DANJJW010000000
VERSION     DANJJW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(107..511)
                     /locus_tag="PWS82_002968"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="PWS82_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="PWS82_002969"
     CDS             complement(570..>959)
                     /locus_tag="PWS82_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908003.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DANJJW010000252          948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000252 DANJJW010000000
VERSION     DANJJW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            659..>948
                     /locus_tag="PWS82_002970"
     rRNA            659..>948
                     /locus_tag="PWS82_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          363 a          161 c          191 g          233 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtacctaat acatgcaagt
      721 cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aagaggagtg gcgaacgggt
      781 gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac aggtgctaat
      841 accgtataac aatcaaaacc gcatggtttt gatttgaaag gcgctttcgg gtgtcgctga
      901 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcacc
//
LOCUS       DANJJW010000253          941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000253 DANJJW010000000
VERSION     DANJJW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..>941)
                     /locus_tag="PWS82_002971"
     rRNA            complement(<1..>941)
                     /locus_tag="PWS82_002971"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          199 a          292 c          211 g          239 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca g
//
LOCUS       DANJJW010000254          931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000254 DANJJW010000000
VERSION     DANJJW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>931
                     /locus_tag="PWS82_002972"
     CDS             <1..>931
                     /locus_tag="PWS82_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0908004.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DANJJW010000255          905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000255 DANJJW010000000
VERSION     DANJJW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            265..888
                     /locus_tag="PWS82_002973"
     CDS             265..888
                     /locus_tag="PWS82_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0908005.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DANJJW010000256          899 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000256 DANJJW010000000
VERSION     DANJJW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            70..>899
                     /locus_tag="PWS82_002974"
     CDS             70..>899
                     /locus_tag="PWS82_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL0908006.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          314 a          134 c          164 g          287 t
ORIGIN      
        1 tagaaaaaag gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag
       61 gacttccata tgaacgattt tactacagaa attatggaaa tactaatcaa taaaggtgat
      121 ttggatgatt tgtttcgtcg tcacctagaa ttggctatta acgctttatt acaagctgaa
      181 ttaacggtat ttcttgacta tgaaaagtat gatagagctg gatttaattc aggtaattcc
      241 cgtaatggga attattcgcg ctcttttaaa acagagtacg gtgaattgaa tttagtaata
      301 cctagagata ggaatggaga cttttctcaa caaactttac ctgcttataa aagaaccaat
      361 gactctttag aaaccactat tattcagcta gttcaaaaag gaattactat gtccgaaatc
      421 tctgatctaa tcgaaaaaat gtatggtcat tattacaaac cacaaactat ttcaaatatg
      481 agtaaaattg tgtctgaaga tgtttcggca tttaaagaaa gaactttaga agctaaatac
      541 tcagtcattt ttatggatgc tactcatatt cccttaaaaa gacaaaccgt atcaaaagaa
      601 gctgtctata tagtaattgg tatcaggttg gatggaacta aagaggttct aggatttact
      661 atagctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagatct aaaagaccgt
      721 ggtttagaag aggttttgtt agtcgtaaca gatggtttaa gtggtattga cgatagtatt
      781 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
      841 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa attagttta
//
LOCUS       DANJJW010000257          899 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000257 DANJJW010000000
VERSION     DANJJW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..170
                     /locus_tag="PWS82_002975"
     CDS             <1..170
                     /locus_tag="PWS82_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0908007.1"
                     /translation="CDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVT
                     FYGMLVENKLL"
     gene            192..>899
                     /locus_tag="PWS82_002976"
     CDS             192..>899
                     /locus_tag="PWS82_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0908008.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQT"
BASE COUNT          366 a           85 c          179 g          269 t
ORIGIN      
        1 tatgtgacga attgattaaa aatttaaata atatatatga tgaaaaagta tggaacttat
       61 taaagaaaga tactttactg tttaagttga catggaaaca atcttttaaa aagcaaaaag
      121 atgataaagt tactttttat gggatgttgg tagaaaacaa attattataa atattagagg
      181 tataaaaagt tatgaaacac aaggtaagtg tgatagtacc aatttacaat gttgaaaaat
      241 atttaagaaa atgtgtggat agtttattga gtcagacatt aaaagagata gaaatcgtat
      301 tagttgatga tggttcgcca gatgcttcgg gggaaatagc agatgagtat caaaaaaaat
      361 attctaatgt aaaaacaata catagagaaa atggggggtt aggaccggca agaaatacag
      421 gaattgagaa tgcgacagga gaatatgtgg cttttcttga ttcagatgat tgggtagaaa
      481 gtgatatgta tgaaaaatta tacttagttg cgtccaaaga aaatgctgat atagtagtta
      541 gtggacattg tgattttgcg aatgacaaag ctatggtgaa aaagcaacat cctttgagtg
      601 ggaatgtata tgatacgcct aatagtatta aaaatgtacg aaaaaattta tttggacata
      661 gtccaaaaga tcaagaagta gaggccttcc cgatgtcagt atgtatgtct ttgtataata
      721 aaaatctgct agataaatat agactaagat ttgaaaaaat tttatctgaa gatacaattt
      781 ttaacttgaa tgcatatgat gtcgcaaata aaattgcttt tacagaatat acagattatt
      841 gttatagaaa agaagaacaa gagtctatta caaaaagttt atctccaaat aagcagacg
//
LOCUS       DANJJW010000258          890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000258 DANJJW010000000
VERSION     DANJJW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..888
                     /locus_tag="PWS82_002977"
     CDS             <1..888
                     /locus_tag="PWS82_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL0908009.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DANJJW010000259          877 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000259 DANJJW010000000
VERSION     DANJJW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            87..356
                     /locus_tag="PWS82_002978"
     CDS             87..356
                     /locus_tag="PWS82_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0908010.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="PWS82_002979"
     CDS             349..606
                     /locus_tag="PWS82_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0908011.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          333 a           97 c          168 g          279 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgctt
//
LOCUS       DANJJW010000260          846 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000260 DANJJW010000000
VERSION     DANJJW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..534
                     /locus_tag="PWS82_002980"
     CDS             <1..534
                     /locus_tag="PWS82_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0908012.1"
                     /translation="NKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQL
                     VSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFN
                     EEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDV
                     EDTANKAQQIVNEHTIQ"
BASE COUNT          379 a          103 c          117 g          247 t
ORIGIN      
        1 aataaagagc aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt
       61 gaacaactaa gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac
      121 aagaatcaat tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct
      181 gaattgcaag gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta
      241 gctgaaataa atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa
      301 tttaatgaag aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt
      361 gaaaaaaata acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag
      421 tcaaaaaata gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta
      481 gaggataccg caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag
      541 ttttaattta acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat
      601 tatacaatct aaacaccgtt tccttttcta tttttgatga catcagtata tagctactcg
      661 aaagagtagt ctttttatta ttctcgctta ttattgatat gaaaatttcg atttgacaaa
      721 aggtaccgaa taattttgta atatgaatta ttatcaagaa gtaattatga tgataacata
      781 tggaaactag ttagtaataa taacaggtga ttaaactttt gaacgacttg taattctata
      841 tttttt
//
LOCUS       DANJJW010000261          841 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000261 DANJJW010000000
VERSION     DANJJW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(86..340)
                     /locus_tag="PWS82_002981"
     CDS             complement(86..340)
                     /locus_tag="PWS82_002981"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0908013.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="PWS82_002982"
     CDS             complement(451..705)
                     /locus_tag="PWS82_002982"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908014.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
BASE COUNT          248 a          152 c          118 g          323 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 t
//
LOCUS       DANJJW010000262          839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000262 DANJJW010000000
VERSION     DANJJW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            66..>839
                     /locus_tag="PWS82_002983"
     CDS             66..>839
                     /locus_tag="PWS82_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908015.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          278 a          155 c          162 g          244 t
ORIGIN      
        1 agacaaagtc cattttatgg atgatgtcta cagtcttttc ttttataata gatttatgaa
       61 aacgaatgca aggagtgata gctatgatgt ctaaaaacaa tacgaatggt agaaatcaat
      121 ttgcgatgct tacgattgat gatctagttc ctcaagatca tctcgttcga aagattgatg
      181 ccgcgcttga ttttgaattt atttatccaa tcgtcgaagc cacttactcc gatttaggtc
      241 gcccaagtat cgacccagtg attctgatca aattggtgtt tattcagtat ttattcggta
      301 tccgttccat gcgacaaacc attaaggaag tcgataccaa tgtcgcttac cgctggtttt
      361 taggctactc cttcgaagag aaaatccccc atttctcaac ttttggaaag aattatgttc
      421 gccgatttag agaaacgacg gtgtttgagg atatttttgc ctatatttta gaacaagcag
      481 ttaaagcagg ttttgtaaca gaggataatt ttcatctaga ttctacccac atcaaagcta
      541 acgccaataa acataatttt acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg
      601 atgaattaga agatgagatc aatcgtcaac gaatagaggc tggaaaacgc ccttttactt
      661 tggacttaga aaaggaagtg aagctgaaag aaagaaaaat aagtaaagct gatccggaaa
      721 gcggctacta cgtcaaaggc gaacaagaaa aacaatttgc ttattctgcc catacttcat
      781 gcgacgacaa tggatttatt cttagcacaa tcatcactcc tggaaatatt catgacagt
//
LOCUS       DANJJW010000263          816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000263 DANJJW010000000
VERSION     DANJJW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            136..309
                     /locus_tag="PWS82_002984"
     CDS             136..309
                     /locus_tag="PWS82_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908016.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaa
//
LOCUS       DANJJW010000264          811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000264 DANJJW010000000
VERSION     DANJJW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..672
                     /locus_tag="PWS82_002985"
     CDS             <1..672
                     /locus_tag="PWS82_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908017.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DANJJW010000265          766 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000265 DANJJW010000000
VERSION     DANJJW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(109..>766)
                     /locus_tag="PWS82_002986"
     CDS             complement(109..>766)
                     /locus_tag="PWS82_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL0908018.1"
                     /translation="IGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQP
                     LEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTD
                     VKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQ
                     FETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
                     "
BASE COUNT          213 a          175 c          146 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgatt
//
LOCUS       DANJJW010000266          751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000266 DANJJW010000000
VERSION     DANJJW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(77..436)
                     /locus_tag="PWS82_002987"
                     /pseudo
     CDS             complement(77..436)
                     /locus_tag="PWS82_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          215 a          138 c          153 g          245 t
ORIGIN      
        1 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactct ttttttcatg
       61 ctctaaaatg atatttttac acaaccgtaa cggttgtttt acgaaattgg acgggacttt
      121 gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag ctatcgatgg
      181 ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct tgttttaata
      241 agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga gacatgcttt
      301 gaaagatttt gtactccttc aatttgtttc tataagcttt catttgatac gaccaacctt
      361 gatctgagtg aaacgtacga cgatagggac aatcgttagt ggtctggata gcttcctcca
      421 aaccttccat taccgcacgt gtttctgaat ggtggtagcg accgatttga acggctaggg
      481 cggaaatacg gtgcaaatgc acggtggttt catgcaatgc ttacagggcg tattttgtaa
      541 gggtatgtaa cggagataat cccgacggta atggatgtga gaggccaagc cgacgctgac
      601 ggtagggcaa acaaacatag ttgcactcta gcgatcgtta tttattttta acggtcaaac
      661 tagggtacaa ctatgcttaa aacacttcgc ctcaacttct ggtagcaaaa gtgattttga
      721 aagtcaaaat attttttatg agttatttat a
//
LOCUS       DANJJW010000267          740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000267 DANJJW010000000
VERSION     DANJJW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            132..344
                     /locus_tag="PWS82_002988"
     CDS             132..344
                     /locus_tag="PWS82_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908019.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>740
                     /locus_tag="PWS82_002989"
     CDS             506..>740
                     /locus_tag="PWS82_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL0908020.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKL"
BASE COUNT          270 a          105 c          126 g          239 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa
//
LOCUS       DANJJW010000268          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000268 DANJJW010000000
VERSION     DANJJW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>733
                     /locus_tag="PWS82_002990"
     rRNA            <1..>733
                     /locus_tag="PWS82_002990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DANJJW010000269          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000269 DANJJW010000000
VERSION     DANJJW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..446)
                     /locus_tag="PWS82_002991"
     CDS             complement(<1..446)
                     /locus_tag="PWS82_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0908021.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DANJJW010000270          639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000270 DANJJW010000000
VERSION     DANJJW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            214..612
                     /gene="spxA"
                     /locus_tag="PWS82_002992"
     CDS             214..612
                     /gene="spxA"
                     /locus_tag="PWS82_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0908022.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          228 a           96 c          121 g          194 t
ORIGIN      
        1 tagatttgaa aactctaatt tttaaatgat aaaaaaagcg agaatgctgg ttttactgta
       61 tattaacctt agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta
      121 aaagttgatt atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa
      181 agtgattctt atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt
      241 tgtacttcct gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa
      301 cgtaatattt tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca
      361 gaagacggaa cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg
      421 gatttagatg atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg
      481 cttcgccgtc caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa
      541 attcgtcggt tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg
      601 gctggattat agaaggataa tacgaaactt ggaaaagac
//
LOCUS       DANJJW010000271          635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000271 DANJJW010000000
VERSION     DANJJW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..270)
                     /locus_tag="PWS82_002993"
     CDS             complement(<1..270)
                     /locus_tag="PWS82_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908023.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="PWS82_002994"
     CDS             complement(276..>635)
                     /locus_tag="PWS82_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0908024.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DANJJW010000272          621 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000272 DANJJW010000000
VERSION     DANJJW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..439
                     /locus_tag="PWS82_002995"
     CDS             <1..439
                     /locus_tag="PWS82_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0908025.1"
                     /translation="SVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAF
                     DVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREI
                     ANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          250 a           73 c          116 g          182 t
ORIGIN      
        1 ttccgtatta ttaattattg gaactggtga tatggaagaa gaaataaaaa attatgcaaa
       61 gaaaagtggc atagatgatg atattaaatt tttaggaaat agaaatgata tagaaaaatt
      121 ttatcaagca tttgacgtat taatgttgcc atcattattt gaaggggttc cgttagtggg
      181 tattgaagct caatttgcag atttaccgtg tttcttttcg gagaaagttc ctacagaagt
      241 tgctttcagt gataagacca atttcataag tttaaatcaa tcggcaaaag aatgggcgag
      301 agagattgca aatgtagata tctctcatcg agattcagaa cgttcgattt taataaaggc
      361 agattataat attcaaacag catacaaaat cttagaatca aaatattatg agttatttga
      421 attaatacaa agaggttaaa gaatatgaca cagaaacatt caagtataaa aagaattata
      481 catcgcatat ccgaagaatt gaatgcaaca agacgaattg caaagataac atcttggaag
      541 gaagcatttg ccacgttaca agctaaaatt gatattcaag tcatgaatcg taatggatat
      601 aatgaaagta aaaaacagaa g
//
LOCUS       DANJJW010000273          606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000273 DANJJW010000000
VERSION     DANJJW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..302
                     /locus_tag="PWS82_002996"
     CDS             <1..302
                     /locus_tag="PWS82_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908026.1"
                     /translation="FMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          229 a           84 c           84 g          209 t
ORIGIN      
        1 ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc
       61 ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta
      121 atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc
      181 ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt
      241 ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa tcaatgtttt
      301 aacttgatca acagaatggg cttttccatt tacacaaaat tattgacgct cccgacaaaa
      361 ttatgatctt gcgtaaagtt ataaaatatt aataaaaata ttttattaat gaaaagcttg
      421 gtagcacggt tattcatatg ctaatttatt tcactttata gtaaggggag ttcttagtat
      481 attgttcaaa cttaaatttc atataattta attatgtaaa ttagctattc taagaacttc
      541 tacggataac tgcccgtcta aaatttaaat aagagcttag aatcttcata taagtaaggt
      601 taattt
//
LOCUS       DANJJW010000274          602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000274 DANJJW010000000
VERSION     DANJJW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            113..>602
                     /locus_tag="PWS82_002997"
     CDS             113..>602
                     /locus_tag="PWS82_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0908027.1"
                     /translation="MDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDL
                     TQYNYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNY
                     SAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDK
                     IIE"
BASE COUNT          235 a           64 c           97 g          206 t
ORIGIN      
        1 aaatctaata agctatggaa aaaagaattc tattaatttt gatataacag cttatagtag
       61 cagtggaaaa aaagagtttg aagaaataac gtatattcaa aatagagacg atttggatac
      121 acattatgat atagctttta ttacaaatcc gacttttaag catctggaaa cactaaatgt
      181 aattgttgaa aaagttgatt acatattttt agaaaaacct gctttttctg agagtattga
      241 tcttactcaa tataattatg attacaaaaa tatttatgtt gctgctccac tgaggtataa
      301 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
      361 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
      421 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
      481 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
      541 tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata aaattataga
      601 aa
//
LOCUS       DANJJW010000275          601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000275 DANJJW010000000
VERSION     DANJJW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(136..>601)
                     /locus_tag="PWS82_002998"
     CDS             complement(136..>601)
                     /locus_tag="PWS82_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0908028.1"
                     /translation="SLIDLDLAKNARFVREHLERQIMLECCAKMNNQKKQVLETIIAE
                     QEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRWLRVKTEGLK
                     WEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPKYFKNI"
BASE COUNT          198 a           95 c           85 g          223 t
ORIGIN      
        1 cgacctcaca tgttttttta ctcaagagaa agtaatataa agtgactata tttgacagaa
       61 ctttacataa aaaaagtagt tagttttctg tagtaatcag aaatgtaaat aaattaaaaa
      121 aaagaaattt agcagttata tatttttaaa atattttggg tgagtagtca atacaacatt
      181 ttgttcatca agcattaaat gaagatgcaa ggatgttaag aagttagctt cttcagggtc
      241 ttgcatggtc aaggctttaa atagttgatg atgttgttcc ataatttttt cccattttaa
      301 tccttccgtt tttactcgta accaacgaaa tcgttccaag tgcgtattat tatcttccaa
      361 ccattgccat acttcttccc gttctgcaat ttcaaaacaa gctttatgaa ataaattatc
      421 attatgaaaa aaactacgag cattcttttg actaacagct ttttcttgtt ctgcaattat
      481 agtttctaaa acttgtttct tttgattatt catcttggca caacactcta gcataatttg
      541 tcgttctaaa tgttctctga caaaacgagc attttttgct aaatctaagt cgattaatga
      601 g
//
LOCUS       DANJJW010000276          565 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000276 DANJJW010000000
VERSION     DANJJW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          181 a          113 c           82 g          189 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatg
//
LOCUS       DANJJW010000277          560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000277 DANJJW010000000
VERSION     DANJJW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            20..199
                     /locus_tag="PWS82_002999"
     CDS             20..199
                     /locus_tag="PWS82_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDL0908029.1"
                     /translation="MLGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNWGQSWSEGL
                     KRWGDNLFGSFSGGR"
     gene            199..549
                     /locus_tag="PWS82_003000"
     CDS             199..549
                     /locus_tag="PWS82_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0908030.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          219 a           62 c          100 g          179 t
ORIGIN      
        1 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcattggta caggaataga
       61 cgttgacgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
      121 aaattgggga caatcatggt cagaagggtt aaaacgttgg ggagataatc tttttggtag
      181 ttttagtgga ggacgataat gacttcaata aacattgatc acacaaaaaa agtcatacat
      241 gaattataca atagtttaat gacacgacct aatcagacga aagcattatt agatattact
      301 gatgttttat ttcaggtata taaaaaaatt gatacagcaa aatatcctga aattcttgta
      361 aataaattag ttaattatat ttacatcgtg ggttttgata ataaaatacg ctttattgat
      421 aatgatgaaa aattattaat agaattaggg gatatttcta aaaaagctgg aattaatagt
      481 aaatacaaag caaactttac agataaatcg caattttata gttattcaga aacaatgcct
      541 caacgataaa atatagtata
//
LOCUS       DANJJW010000278          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000278 DANJJW010000000
VERSION     DANJJW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWS82_003001"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWS82_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL0908031.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="PWS82_003002"
     CDS             complement(349..>526)
                     /locus_tag="PWS82_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908032.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DANJJW010000279          516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000279 DANJJW010000000
VERSION     DANJJW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>516
                     /locus_tag="PWS82_003003"
     CDS             <1..>516
                     /locus_tag="PWS82_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL0908033.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQ
                     AAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQD
                     HRPVKRRNKFYR"
BASE COUNT          180 a           88 c          101 g          147 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt
      241 gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga
      301 cacacaagca gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt
      361 tgtagtcaca gataaagccc cctctattac aagtgccttt aagaaactaa aagaatacgg
      421 cttttatcaa gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga
      481 ccatcgtcca gtaaagagac gcaataaatt ctatcg
//
LOCUS       DANJJW010000280          505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000280 DANJJW010000000
VERSION     DANJJW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>505
                     /locus_tag="PWS82_003004"
     CDS             <1..>505
                     /locus_tag="PWS82_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0908034.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKG"
BASE COUNT          190 a           55 c           84 g          176 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aagga
//
LOCUS       DANJJW010000281          485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000281 DANJJW010000000
VERSION     DANJJW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(62..259)
                     /locus_tag="PWS82_003005"
     CDS             complement(62..259)
                     /locus_tag="PWS82_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0908035.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DANJJW010000282          484 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000282 DANJJW010000000
VERSION     DANJJW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          159 a           96 c           57 g          172 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatg
//
LOCUS       DANJJW010000283          478 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000283 DANJJW010000000
VERSION     DANJJW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(45..>478)
                     /locus_tag="PWS82_003006"
     CDS             complement(45..>478)
                     /locus_tag="PWS82_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0908036.1"
                     /translation="LNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIG
                     KTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDL
                     NKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          170 a           72 c           24 g          212 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaat
//
LOCUS       DANJJW010000284          475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000284 DANJJW010000000
VERSION     DANJJW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(268..414)
                     /locus_tag="PWS82_003007"
     CDS             complement(268..414)
                     /locus_tag="PWS82_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL0908037.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          166 a           77 c           54 g          178 t
ORIGIN      
        1 tatattagtt ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc
       61 ggaaacatca gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag
      121 atattttctt ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt
      181 tcccacgaac attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc
      241 tgattttagg atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca
      301 ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt
      361 tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat
      421 tccccctaca aactataaat acttttatgt cactgattca atatacctat ttttc
//
LOCUS       DANJJW010000285          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000285 DANJJW010000000
VERSION     DANJJW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..430)
                     /locus_tag="PWS82_003008"
     CDS             complement(<1..430)
                     /locus_tag="PWS82_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0908038.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANJJW010000286          440 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000286 DANJJW010000000
VERSION     DANJJW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>440
                     /locus_tag="PWS82_003009"
     CDS             <1..>440
                     /locus_tag="PWS82_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0908039.1"
                     /translation="KTYDYSQNQIINQSKSPYSNCIWVCWWQGLDQSPEIVKACVKSI
                     KRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWL
                     DATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASGYFAGYSLAC"
BASE COUNT          164 a           59 c           83 g          134 t
ORIGIN      
        1 aaaacatatg attatagtca aaatcaaata attaatcaat caaaatcacc atattctaac
       61 tgcatttggg tttgttggtg gcagggattg gatcaatcgc cggaaattgt aaaagcttgt
      121 gtaaaatcaa taaaacgaaa tgcagggaaa catactgtta taattttaac agaagataat
      181 tatagacaat atgttgatat accaaaatgg gtggaagaaa agaaaaacaa aggaatcatt
      241 acgagaacaa attattctga tttattaaga ttatctttgc tagcaaaaca tggaggtttg
      301 tggttagacg ccacatttta ttgtacaaaa ccaataccag atgagtattt tgatatcgat
      361 atttggtcta ttaggaggcc tgattatgct cacgcatccg tagcatcagg atattttgcg
      421 ggttattcat tagcatgtaa
//
LOCUS       DANJJW010000287          429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000287 DANJJW010000000
VERSION     DANJJW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..351
                     /locus_tag="PWS82_003010"
     CDS             <1..351
                     /locus_tag="PWS82_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0908040.1"
                     /translation="KKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVD
                     FQSKQKCKIKFVPTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWM
                     KKMDKQGEYLYGED"
BASE COUNT          184 a           37 c           74 g          134 t
ORIGIN      
        1 aaaaaagaat ctcttgcctt tataatgttt actcaagaag agtgtgagta ttgtatagca
       61 ataaatccaa tcatagatag tgtaaataag gaaactttga ataagataaa gaaatatgat
      121 atatctgtag attttcaatc taaacaaaag tgtaaaatta agtttgttcc gactattgtt
      181 gcatatagta agaataaaga agtggggaga attgacaatg aattagataa tttgtatcga
      241 aaaaagcaag atggttcaat tgtaatagac aagaaaaaaa caaagacatt gcttatagat
      301 tggatgaaaa aaatggataa acaaggagaa tatttgtatg gagaagatta aacagacatt
      361 tccgttaatt aataaatatt ggaataagaa aaaaattttt atatttttta ttacgtttct
      421 gctgtcatc
//
LOCUS       DANJJW010000288          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000288 DANJJW010000000
VERSION     DANJJW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..339)
                     /locus_tag="PWS82_003011"
     CDS             complement(<1..339)
                     /locus_tag="PWS82_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0908041.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANJJW010000289          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000289 DANJJW010000000
VERSION     DANJJW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>404
                     /locus_tag="PWS82_003012"
     CDS             <1..>404
                     /locus_tag="PWS82_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908042.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANJJW010000290          366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000290 DANJJW010000000
VERSION     DANJJW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(34..252)
                     /locus_tag="PWS82_003013"
     CDS             complement(34..252)
                     /locus_tag="PWS82_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908043.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          128 a           66 c           64 g          108 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatgcgaa caattgttcg tatatttagt
      301 atacgaatat ttgttcggtt tgtaaagagg gaagtgataa aaatttgaaa gaagaagaac
      361 ccgggc
//
LOCUS       DANJJW010000291          347 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000291 DANJJW010000000
VERSION     DANJJW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          154 a           57 c           37 g           99 t
ORIGIN      
        1 ttgctgttca tattgttctc gttgcctaat agttattcta ataaataaaa atagaacaac
       61 aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa atacttgata
      121 ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca tgaatatagc
      181 agtaagttta gaactggact gttcaacaat cctatttata gctaattttc tatataacca
      241 aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat aaccataccc
      301 ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaa
//
LOCUS       DANJJW010000292          338 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000292 DANJJW010000000
VERSION     DANJJW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            1..338
                     /locus_tag="PWS82_003014"
                     /pseudo
     CDS             1..338
                     /locus_tag="PWS82_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DANJJW010000293          331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000293 DANJJW010000000
VERSION     DANJJW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>331
                     /locus_tag="PWS82_003015"
     CDS             <1..>331
                     /locus_tag="PWS82_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HDL0908044.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DANJJW010000294        17129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.056, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000294 DANJJW010000000
VERSION     DANJJW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..2121
                     /locus_tag="PWS82_003016"
     CDS             <1..2121
                     /locus_tag="PWS82_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0908045.1"
                     /translation="FFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVS
                     MPMAEIERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPK
                     RYEDKKVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSE
                     IFYYQQQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVD
                     ALESVDELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPF
                     GDMLGLHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRN
                     IYLQPSLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAE
                     RGEWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISS
                     RDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLL
                     FSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFI
                     HSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMK
                     NNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQ
                     FHPIFEELLHAFDTRPPVRNEVEE"
     gene            2118..4145
                     /locus_tag="PWS82_003017"
     CDS             2118..4145
                     /locus_tag="PWS82_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908046.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            4142..5164
                     /locus_tag="PWS82_003018"
     CDS             4142..5164
                     /locus_tag="PWS82_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0908047.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            5181..6110
                     /locus_tag="PWS82_003019"
     CDS             5181..6110
                     /locus_tag="PWS82_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0908048.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            6355..6471
                     /locus_tag="PWS82_003020"
     CDS             6355..6471
                     /locus_tag="PWS82_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL0908049.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            6487..7290
                     /locus_tag="PWS82_003021"
     CDS             6487..7290
                     /locus_tag="PWS82_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0908050.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            7525..7761
                     /locus_tag="PWS82_003022"
     CDS             7525..7761
                     /locus_tag="PWS82_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908051.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            7768..9720
                     /locus_tag="PWS82_003023"
     CDS             7768..9720
                     /locus_tag="PWS82_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0908052.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(9792..10289)
                     /gene="dfrG"
                     /locus_tag="PWS82_003024"
     CDS             complement(9792..10289)
                     /gene="dfrG"
                     /locus_tag="PWS82_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0908053.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            10651..11691
                     /locus_tag="PWS82_003025"
     CDS             10651..11691
                     /locus_tag="PWS82_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0908054.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            11789..11977
                     /locus_tag="PWS82_003026"
     CDS             11789..11977
                     /locus_tag="PWS82_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL0908055.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(12035..12388)
                     /locus_tag="PWS82_003027"
     CDS             complement(12035..12388)
                     /locus_tag="PWS82_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0908056.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            12919..13341
                     /locus_tag="PWS82_003028"
     CDS             12919..13341
                     /locus_tag="PWS82_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0908057.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            13338..13568
                     /locus_tag="PWS82_003029"
     CDS             13338..13568
                     /locus_tag="PWS82_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0908058.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            14066..14266
                     /locus_tag="PWS82_003030"
     CDS             14066..14266
                     /locus_tag="PWS82_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0908059.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            14293..15486
                     /locus_tag="PWS82_003031"
     CDS             14293..15486
                     /locus_tag="PWS82_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0908060.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(15554..17119)
                     /gene="guaA"
                     /locus_tag="PWS82_003032"
     CDS             complement(15554..17119)
                     /gene="guaA"
                     /locus_tag="PWS82_003032"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0908061.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         5723 a         2973 c         3274 g         5159 t
ORIGIN      
        1 ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa aaatgttggg
       61 aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa attaatgggc
      121 gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga acagttgtta
      181 gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga ttttggttat
      241 attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga ctaccattta
      301 ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct caaacccact
      361 cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga taccactact
      421 tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc taatagtgaa
      481 attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat gaatgttgaa
      541 attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga actgaaagat
      601 ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt ggacgcttta
      661 gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat gtacaaacta
      721 agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg ttgtgatgaa
      781 gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg cgatatgcta
      841 ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta tattcaatat
      901 gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat tggtgaaaat
      961 tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct ccaacctagc
     1021 cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc ttttattggt
     1081 tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc agtactcttt
     1141 ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa agaaacctta
     1201 cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga caataaaggg
     1261 ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct tgccattgat
     1321 attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc tgtcctgcgt
     1381 aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt gattgatgaa
     1441 ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga aagctttgtc
     1501 gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc cattagcttg
     1561 gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc caatagctct
     1621 tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt cattagtacc
     1681 tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga ccttgatgag
     1741 gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt ggttcgagca
     1801 ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga tttactagat
     1861 gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga cctagtggaa
     1921 atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa tcagaaacgc
     1981 ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg tgtaggggtt
     2041 atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag accgcctgtg
     2101 cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat agttggataa
     2161 cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat gcagtacaag
     2221 cgacaggctt agttgatgaa accattgata caagcaatga atactccaag tatggtttga
     2281 atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca tggaattgga
     2341 gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat tttatttgga
     2401 caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc tatgccttag
     2461 atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt gcaggcatta
     2521 caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt tttattctca
     2581 ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg aaagtgatac
     2641 gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt gcgtatgcac
     2701 ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc agtctgaata
     2761 ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt gtggacatga
     2821 tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt caatatggct
     2881 ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct accagtccag
     2941 atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa gatcatgaca
     3001 atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc tttttgtttg
     3061 tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg attttttcgc
     3121 aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta agtatgttgc
     3181 cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg attatgacaa
     3241 gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg ctttattcgc
     3301 tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc tttgcaggga
     3361 tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac gactctcaaa
     3421 acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa accagaagat
     3481 ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag caaaagacaa
     3541 tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat gaacggacta
     3601 caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt atcgggcaac
     3661 gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc aataaaagaa
     3721 agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc cttcatcaag
     3781 gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca gataccaaag
     3841 ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac attgctcaaa
     3901 agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca tcatctcctg
     3961 ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct gttttgtcac
     4021 aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa caaaagacac
     4081 caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga ggacaacaaa
     4141 catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct tttttctctt
     4201 attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca acaaggattc
     4261 ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc gtccgttgat
     4321 tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct tagccattat
     4381 gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat ctttgggttt
     4441 accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat atttcagtga
     4501 attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc aatcttataa
     4561 ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata cctatgaatt
     4621 agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc ccaatcctat
     4681 agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt atgttcagtt
     4741 ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa ccgtgcaagt
     4801 cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag gggcgtcgcc
     4861 taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag ctggcatttc
     4921 tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta tggaagaagc
     4981 ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat atgtgactca
     5041 tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa tcggttatgg
     5101 cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg ttattcataa
     5161 ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag aaggagaaac
     5221 caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa gttggcacgc
     5281 ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga tttggaatct
     5341 ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc attaaacagc
     5401 agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa gactactttt
     5461 cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc cattatttga
     5521 cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct actagttcta
     5581 gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc tttgaggtac
     5641 tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata tcgtctagct
     5701 ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa aatccgacaa
     5761 tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt gaccatacag
     5821 tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc cagctctatc
     5881 ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta cctatgatta
     5941 acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa gataaccaag
     6001 taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc caaatctcgc
     6061 aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa caaatattgg
     6121 tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct
     6181 ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata
     6241 tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt
     6301 ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt
     6361 ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc
     6421 agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa
     6481 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg aaaaactacc
     6541 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     6601 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     6661 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     6721 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     6781 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     6841 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     6901 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     6961 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     7021 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     7081 gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc tctttatcat
     7141 ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac taataaattt
     7201 tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa aattgaatat
     7261 acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt tttggagtac aaaaagtgaa
     7321 aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt tttattgtta
     7381 aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc
     7441 gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt acaatttact
     7501 aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa gaacgctaag
     7561 tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata cgctgaatat
     7621 gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt agacgataag
     7681 aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga aaaaatgggt
     7741 atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc cacgaaggaa
     7801 gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga tagggctaaa
     7861 caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat gttgttcttg
     7921 ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta ctgcatcaat
     7981 ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa agggaaacca
     8041 agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat gtactgcaat
     8101 gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt atctaattgg
     8161 tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga tgttgagcca
     8221 gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt caatgagccg
     8281 aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg
     8341 tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta tcaccaacaa
     8401 aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc agtcagtcgt
     8461 acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga gtggctgtat
     8521 aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac aaagaaattt
     8581 aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt ccgtaagaaa
     8641 ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac gtatatagtt
     8701 gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta tgattggaat
     8761 atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca tctcaatact
     8821 tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc atccgctaac
     8881 gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa taggagtaga
     8941 tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta ttggttaatt
     9001 aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt ctctattaga
     9061 aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca tcattttatt
     9121 tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa
     9181 gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct acggactttg
     9241 gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga ggctattagt
     9301 aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc attagccact
     9361 aaggataaag gattttattc agttgattgt agaacggatt tatctgctct ggaaccaaac
     9421 gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt tattcaacag
     9481 ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg tgataagaag
     9541 agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac aattctaaga
     9601 acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt gacacctgct
     9661 caaagattag ggattacaga taaacaattt gattggaaag atataattta tttcaaatga
     9721 caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat
     9781 taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac
     9841 ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc
     9901 acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc
     9961 tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct
    10021 tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag
    10081 gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt
    10141 gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat
    10201 tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta
    10261 tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac taatttacca
    10321 tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa
    10381 gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt ttcattcaat
    10441 tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg taagttaaaa
    10501 tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa actgaactac
    10561 ttacttgcat atatacgcct ttatagtgga gtactacatt tacgagattc ggttagagta
    10621 tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt
    10681 aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta
    10741 aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac
    10801 cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat
    10861 gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca
    10921 catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg
    10981 caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga
    11041 ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct
    11101 tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg
    11161 gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat
    11221 ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac
    11281 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
    11341 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
    11401 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
    11461 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
    11521 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
    11581 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
    11641 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
    11701 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
    11761 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
    11821 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
    11881 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
    11941 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
    12001 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
    12061 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
    12121 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
    12181 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
    12241 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
    12301 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
    12361 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
    12421 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
    12481 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
    12541 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
    12601 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
    12661 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
    12721 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
    12781 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
    12841 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
    12901 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
    12961 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
    13021 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
    13081 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
    13141 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
    13201 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
    13261 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
    13321 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
    13381 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
    13441 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
    13501 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
    13561 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
    13621 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
    13681 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
    13741 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
    13801 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
    13861 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
    13921 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
    13981 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
    14041 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
    14101 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
    14161 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
    14221 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
    14281 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
    14341 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
    14401 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
    14461 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
    14521 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
    14581 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
    14641 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
    14701 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
    14761 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
    14821 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
    14881 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
    14941 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
    15001 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
    15061 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
    15121 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
    15181 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
    15241 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
    15301 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
    15361 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
    15421 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
    15481 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
    15541 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
    15601 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
    15661 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
    15721 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
    15781 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
    15841 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
    15901 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
    15961 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
    16021 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
    16081 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
    16141 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
    16201 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
    16261 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
    16321 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
    16381 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
    16441 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
    16501 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
    16561 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
    16621 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
    16681 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
    16741 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
    16801 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
    16861 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
    16921 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
    16981 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
    17041 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
    17101 tatcggcaac gtttgtcaaa tttattacc
//
LOCUS       DANJJW010000295        17118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.057, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000295 DANJJW010000000
VERSION     DANJJW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(58..1287)
                     /gene="hflX"
                     /locus_tag="PWS82_003033"
     CDS             complement(58..1287)
                     /gene="hflX"
                     /locus_tag="PWS82_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL0908062.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1289..2206)
                     /gene="miaA"
                     /locus_tag="PWS82_003034"
     CDS             complement(1289..2206)
                     /gene="miaA"
                     /locus_tag="PWS82_003034"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL0908063.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2210..2947)
                     /locus_tag="PWS82_003035"
     CDS             complement(2210..2947)
                     /locus_tag="PWS82_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0908064.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3038..3565)
                     /locus_tag="PWS82_003036"
     CDS             complement(3038..3565)
                     /locus_tag="PWS82_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908065.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3745..4338
                     /gene="clpP"
                     /locus_tag="PWS82_003037"
     CDS             3745..4338
                     /gene="clpP"
                     /locus_tag="PWS82_003037"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL0908066.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4442..4927)
                     /locus_tag="PWS82_003038"
     CDS             complement(4442..4927)
                     /locus_tag="PWS82_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL0908067.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5080..5277)
                     /locus_tag="PWS82_003039"
     CDS             complement(5080..5277)
                     /locus_tag="PWS82_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0908068.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5372..7132)
                     /gene="efrB"
                     /locus_tag="PWS82_003040"
     CDS             complement(5372..7132)
                     /gene="efrB"
                     /locus_tag="PWS82_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL0908069.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7129..8859)
                     /gene="efrA"
                     /locus_tag="PWS82_003041"
     CDS             complement(7129..8859)
                     /gene="efrA"
                     /locus_tag="PWS82_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL0908070.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9252..9464
                     /locus_tag="PWS82_003042"
     CDS             9252..9464
                     /locus_tag="PWS82_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0908071.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9466..11145
                     /locus_tag="PWS82_003043"
     CDS             9466..11145
                     /locus_tag="PWS82_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0908072.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11399..11599)
                     /locus_tag="PWS82_003044"
     CDS             complement(11399..11599)
                     /locus_tag="PWS82_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0908073.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11749..11892)
                     /locus_tag="PWS82_003045"
                     /pseudo
     CDS             complement(11749..11892)
                     /locus_tag="PWS82_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12127..12789)
                     /locus_tag="PWS82_003046"
     CDS             complement(12127..12789)
                     /locus_tag="PWS82_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0908074.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13181..13240
                     /locus_tag="PWS82_003047"
     CDS             13181..13240
                     /locus_tag="PWS82_003047"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL0908075.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13298..14029
                     /gene="erm(A)"
                     /locus_tag="PWS82_003048"
     CDS             13298..14029
                     /gene="erm(A)"
                     /locus_tag="PWS82_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL0908076.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14155..14937)
                     /locus_tag="PWS82_003049"
     CDS             complement(14155..14937)
                     /locus_tag="PWS82_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL0908077.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(15091..15468)
                     /locus_tag="PWS82_003050"
     CDS             complement(15091..15468)
                     /locus_tag="PWS82_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0908078.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(15475..>17118)
                     /locus_tag="PWS82_003051"
     CDS             complement(15475..>17118)
                     /locus_tag="PWS82_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0908079.1"
                     /translation="GIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTE
                     YRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGEEWDKD
                     VWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQ
                     RLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTL
                     LRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQE
                     CGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSE
                     EFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRM
                     INNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDN
                     EVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQ
                     LERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
BASE COUNT         5090 a         3550 c         2813 g         5665 t
ORIGIN      
        1 aatggaaagt tttgtctcgc ttaatttgac aaataatatt gttaaaaaaa taaaatttta
       61 ttggtttttt cttaaccatt ttgaattggt ttttgcaaac cctttaactt catattcgtt
      121 tgtttcttcc atgtacgctt cgtccaaaac cattgtttgt cttctcaact cgcttaattt
      181 ctgtccttcg ttgcttggca cagaaagtgt ataaggggtc attagttcca tcagctgacg
      241 tttgattgct tcgatcaacg ccatcttacc ttctttcgtc tgagtagaaa tcaatacatt
      301 tgggaatagc gtaggcgtga acatcgctgg gtcgatctgg tctgccttat tgtaaacagt
      361 cagtatcggc atttctttca tattcagttc atccatcaat tttaagacag tctgctcctg
      421 taaaatacga tctggagaac tagcatcgac tacatgtagc aaaagatcca tattttgact
      481 ttcttctaat gtagaatgga aagcatcgat cagttgcgta ggcaggtctt ggatgaaccc
      541 gacggtatcc gttacagtaa tctcaaatcc ttcagcaaaa cgccatctct tagtcaatgg
      601 atctaacgtt gcaaacaatt gatctttcga ataagtatcc gcttgtgtca atagattcaa
      661 gatagttgac ttcccggcat tcgtgtagcc aataagtcca atctgaaaaa tctcgctatt
      721 ttttcttttt tggcgattgc gctctcgatg agcttctact tctttcaatt ctttcttaac
      781 agcaaaaatt ttattccgta tatgtcggcg gtctgtttct aactttgttt cacctggccc
      841 tcttgttccg atcccaccgc caagacgaga taatgttttc ccttgcccta ctaaacgagg
      901 cagcaggtat tctaactgag caagttcaac ctgcagcttc ccttctttcg aacgagcacg
      961 catcgcaaaa atatctaaga taagctgcac gcgatcaatt accggaagac caacagcatc
     1021 ggtaatcagt tggctctgtc tcggagttag ctcatgatta aagatgatca aatccgcttc
     1081 atgcgcatcc gcttgttgaa ctaattcttg taatttccct ttaccgataa tcgtttgccg
     1141 atccacttgt ggtctctttt gtgtaattgt gaatacgact tctcctgatg cggtattcgt
     1201 cagctgaact agctcttcca ttgactctga aaagtaacgc tggtttgctt cggtttctac
     1261 ccctacgatg atcacttttt cttccatttt attccgcctc cagccatttt ttgattgttt
     1321 gctcaatctg acttttttga tttggctcac ttataagatc aaaccactgt gcatccaaac
     1381 ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct cgaatgtagt ttgacttgtt
     1441 ctgttgcttc ttccaatgtt tctttccctt caaaataagg gaaaaattct ttatatccaa
     1501 taccttgact ggcctggact tctccaagct catacacctt acgcgcttct gctactaaac
     1561 cttctgctag catcaaatcg actctttgat tgattcgttc ataaagaata ctacgcttcg
     1621 tatctaatcc aatcatgagg taatcataca attgctttgg ctcttcttta ggtgccgtga
     1681 tactatgtcc tgtcgtctct aaaacttcca gagctcttat cactttgcgt tgattgttcc
     1741 agtggatttt ctcagcagct gccgggtctt tttctttcaa ctgattccat aatgcttggg
     1801 aaccaattgt ttcagctaac gtttcatact tttttctaat ctcaggattc gcttcttctg
     1861 tcgctcctaa ctgataatca tatagaaggg attggatata gagacccgta ccaccagcta
     1921 tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa ttgtcgacct gaatgctgaa
     1981 aatcagcggc tgagtaacgt tgagtaggtt cgatcacatc aatcaaatga tgcggaacat
     2041 tattcatctc ttcttctttg atttttgctg taccgatatc cagattttta tagatttgca
     2101 atgaatcacc actgatgatc tctcctccaa atagttgtgc gagttccaca cttagcgctg
     2161 ttttaccgac tgctgtcgga ccgacgatca ctaatacttt ttccatattt tacctacttt
     2221 tcgttgattg tttccaccga agagctttct ccggaaaatc agttattatt ccatctattt
     2281 tgagttcttg acatttggcc atttcttctt catcgttaat cgtccatgca cgaattgctt
     2341 ttggaaaatc agctgcctgg actggatgtt cgaacaacca agtatttttt ggatgtactc
     2401 cttctatata aggggtttct gctgctagtt ggatcttttt atcagaagta ctcatcaata
     2461 agtccagctg tatctccggt tccagtaatg cgattttttc caaagaatcc aaatgaaagc
     2521 tgcaatatgc atgttgaaaa ggccaatctc tactttttaa tgtttcggct aatttttctt
     2581 cgataccggg atattcatac tgatcagttt taatttcaat cattagcatc cctcggtaat
     2641 ttttttggag gagcagtcct aacacttctc tcaattcagg aactttagct gctggaaatt
     2701 ctttgctgaa tttgccccct gcattcaatt ttttgagttc ttccaatgtt ttttctcgaa
     2761 tcagcccttt accgttagtc gtccggtcca cttcttcgtc gtgcataacg atcaaataac
     2821 catctttgct tagatggaca tctagttcga taccatctgc acctactcgt acagcttctg
     2881 caaatgcagg aagcgtattt tctgggtggg tgccgctgct tccgcgatgg gcatagattt
     2941 gtaacatctt tcttcactcc gttcagttct attgtatcat taagaaaaaa agaaagcatt
     3001 accacgctct catgtgcaac ggcaatgctt caaaatatca gctccaaaca tcatctttcg
     3061 agaagaaacc aatcggctgg cgcgtcatag tcttcaactg agcttttttt tcattgatca
     3121 tttcagtcag actatccgta aaattctcta aaatcacgcg tccgccttta taagcatatc
     3181 caccaatctt cattgtttca tctggagata aaatcacttt tcgatgggag atattcttaa
     3241 atagctcgtc taaaatataa tttggcagat aaaaactatt ttgcgctgaa aatgattgga
     3301 gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa tgcatcaaag ctgacatatt
     3361 cttccatcag cgttgtccgt ttttgcaaga tctcgttgat ctttttattt aattgattca
     3421 gcgtctcgat ttctttgact ttcaattcca attcacgtct acgctgttct ttattttcca
     3481 agtgacgcat ttgcagtatc atgccgatat agacaactac agcacctgtc agcactccga
     3541 taaggccagt aataccaact atcaatctgt tgttcctcct tgttttgttt ctctgcttat
     3601 cataccataa agcgggttta tgcgactaat ttgcctaagt tttttatttg accttttttg
     3661 accaaaaatg tatactacat tttaggtatt agaaactaaa ttatggtaca ctatttctaa
     3721 acgaaatcaa ggaggaactc tattatgaat ttaattccta cagtcattga acaatcttca
     3781 cgaggtgaaa gagcttacga tatctactca cgtttattga aagaccgtat catcatgtta
     3841 agcggccaag taacagatga tcttgctaat tcgatcatcg cccaattgct tttcttagac
     3901 gctcaagatt ctgaaaaaga tatctatctt tatatcaatt ctcctggtgg ttcagttaca
     3961 gcaggtatgg ctatttacga cacaatgaac tttgtcaaag cagatgtcca aacaattgtt
     4021 atgggtatgg ctgcttcaat gggaagcttc ttgctgacag ctggtacaaa aggtaaacga
     4081 tttgctttac caaatgctga gatcatgatc catcaacctc ttggcggcgc tcaagggcaa
     4141 gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa cacgcgaacg cttaaacaaa
     4201 attttagctg aacgcactgg acaaccgctt gaagtgattg aaaaagatac agatcgtgac
     4261 aactatatga cagctgaaca agcaaaagca tatggattga tcgatgaagt catggaaaac
     4321 agttcttcat taaattaatc tgtttcaaag gaagcttttt taagctctct tatctaaacc
     4381 aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt ttattttatg gatgctactg
     4441 cctatctttg ctccttgaat tttccttgcg catgcatccg acctatctcg acataatgct
     4501 ggatgttttc tttgtttttt tgtatttctt cttttgtcaa aggacggatc actttagctg
     4561 ggcgaccaaa agccaagaca ttaggaggaa taacttttcc ttctgttact aatgatccag
     4621 caccaatcaa actattctca ccaataaccg catgattcaa tatcgtggtc cccatcccga
     4681 tcagcgcccc tttttcgatt gtacagccat gtagcatgca ctgatgtcct accgtcacat
     4741 tctccgcaat atctacaggt gcatcatgat cgacatggat gattgtacca tcttggatat
     4801 tcgttctttg tcctattttt atccaattac tgtccccgcg taaaacagct tgataccaga
     4861 tcgtcacatc ttctgacaat gtcacatcac caatcactgt ggcatttgat gctataaacc
     4921 gttccatatt tatttttctc ctttcatttt ttgtagaaac taatcctatt gaataaaggt
     4981 atcatagcat atatttattt cataagaaaa aaaacaggta aaacctgctc aggccagatg
     5041 tctatttaca gtatctggta actgacgttt tacctgtttt tattgtacat ctatattttc
     5101 ttctattact ttttttaaaa ttaattcgta cacttctatt tgtgctgcca agcgttcgat
     5161 ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc ttcgtctgac agacgtgcgt
     5221 taaacgatca acttctcttt tggcttcagt aattttttca tttaaaaact gttccatctc
     5281 cgtgtcctca cttttcttga taactttact ttaccattat caagaataga gaacaaaaaa
     5341 taagttttaa actgttttat tcttatatga cttattctgc ttcctcagca aattggctgt
     5401 tatacagttt ttcataaaat ccaccttgtg ccaataaact ctcatgcgtt cctttttcaa
     5461 tgatctgacc ttgatccatc actagaatca gatctgcatc acggattgtc gataaacggt
     5521 gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc cattgctttt tgaatcaacg
     5581 cttctaaacg ggtgtctact gaacttgttg cttcatctaa aatcaagatt ttaggatcgg
     5641 agaccactgc tcgggcaatc gtcagcaact gtttttgtcc taaagacaca ttgtctccct
     5701 cagaattgat ggccatatca tatccatctg gcatcgttcg gataaaatga tctacgtttg
     5761 ctgttttcgc agcatcaact acttcataat ctgtcgcatc aagctttccg aagcggatat
     5821 tatctgcaat cgttccttga taaagccatg catcttgcaa gaccatacca aatagcgaac
     5881 gcacatcact gcgactcatt gatttggtgt cgattccatc aatccttatt gctcctttgt
     5941 ctacgtcata aaaacgcatc aatagattga tcaatgtcgt cttgccggca ccggtcggac
     6001 cgaccacagc tacggtttga ccagctttca cttcaaaatt caaatcttgg attaatggtt
     6061 tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac atctcctaaa atcgtttctg
     6121 gcagtgaagc atctactttg ttttcttttt cttctggctc atctaaaatc tcgaatacac
     6181 gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa ttgtgtcacg ttccccattg
     6241 gctgactgat ttgccaaata tattggatga atgcttgtag ttgaccgaca acgatcgccc
     6301 cagtaataac ataaaagctt cctagaacag ccataccaat ataagtagca taggcagtta
     6361 attgaactag aggcatcatc aagccagaaa tgaatgctgc acgaaaccca aagtatttca
     6421 agctgtgatt aacttttttg aatccttcca atgtttcttt ttcgcgtcca tatactttca
     6481 atacactaaa acctgtcata ttttcttgga cgtagccgtt tagctcacca agagaattct
     6541 gcatgctttg aaaatatttc tgcgaagcat tgatcactcg ttttgagatc cagatcgatg
     6601 ttggaatcat gatcatagag atcagcgcca tcatcggctg gatataaaac atcattgctg
     6661 cagccaacgt aattcccaat atcgcattta cgataccaat aaaagcttgt tgtaaagcac
     6721 cgctgactgc atctacatca ttcgtcacac gagacaaaat atttccttgc tggtttttat
     6781 cgaaatatga aaccggaaga cgattgattt tttcttctat gtctcttctc aaatcatgca
     6841 tggcactttg aacgacattt gtcatcaaaa caccagaaag taattgcgaa atacaatacc
     6901 ctattccaac aactgtgatc caaatgagac atttgaatat ataagaaaaa ttgattgatt
     6961 cgccattggc aatattacgg ctgatttctg tagtaggaag tcctgtaaca tatggcaaag
     7021 ccacgttgaa agccactgtc agaactgtaa ataggatcac taaaagaaaa gtctcacgat
     7081 acggtctgat atatcgagct aaacgtttta aggaagaaaa ggtttttttc atgctaattc
     7141 ctcctttgat aattgagaag cggcaatatc ataataaatc gggcattttt ccaacaactc
     7201 ccgatgcgtc cccataccga cgacttctcc ttcatttaat acgatgatct tatctgcatg
     7261 catgatcgtt cccacacgtt gtgctacgat caatacagta gattctgtcg tttctttttt
     7321 cagacgagca cgtaacttcg catccgtttg ataatctaat gcagaaaagc tgtcatcaaa
     7381 aatatagatt tccggacggc gtatgatggc tctggctatt gccaatcgtt gtttttgtcc
     7441 acctgaaaaa ttggaaccac cttctgataa atgttcatta aatccatctg gttttcgaga
     7501 aatgaattct gaagcttgag caatctcagc tgcacgttcc atttcttctt gcgtagcatc
     7561 ttctttcccg tatcgcaaat tatctgcaat cgttccagta aatagcagcg ctttttgcgg
     7621 aataaaccca atcttatctc gcaacttgct tagccgataa tctctcacgt ctactccatc
     7681 gatcaagatc tttccttcac tcacatcata aaagcgaggg atcaattgga tcaacgtaga
     7741 ttttccgcta ccggtacttc caataaaagc aacggtctct cctggagagg ctgtaaaact
     7801 gacatttcgt atcactggct cttgcgaatg gcctggataa gcaaatgtta cattttgaaa
     7861 ttcgatatac cctttagttt ttgtatcaga tataccattt ggatcttcta caatcgaagg
     7921 tttggcatct agggcttctt ggatacgtcc agctgaaaca gctgctcttg gatacatcat
     7981 gaacacacta gcaaataaca tgaatgagaa caaggcatgg aatatatact cgataaacgc
     8041 aatcaaatct cctacacgaa gctcgcctgc atcgatttgc actgccccgc tccagatgat
     8101 caataccatt acgatattaa ataagaaata aaaacccggt tgtgcaacag ccatcaaacg
     8161 gaataagctt tttgaactag aagcataatt ctcattgact acagcaaaac gtttttcttc
     8221 aaacttttca ttgacgaatg cacgaatcac acgcaatcct gaaagatttt cccgcaagat
     8281 actgttgatt ttatctaagt ttttttgttg ctttttagat aacggttcag acaccttagc
     8341 aatcgctacg actgccaata atagtaaagg taatgcacct actacataca aactgagtgt
     8401 cacacttgta cgcatgacca tgtacaaact gacaatgaac atcattggtg ccataaaacc
     8461 gatacgtaaa atattttgca ggaacaacat gatctgataa gcatcgtttg ttgtacgagt
     8521 gatcaatgaa gatacgccta gattttcgta ttcctgatgc gaataacttt ggatcttttc
     8581 gaacaaatca tctcgaatat ctgctacgat attagtcgta atcctcgaac caaaataacc
     8641 taacaagata ttcaaaataa taccgattac agtaataacg atcataaata tcccttgttg
     8701 cttcacataa tcaaaatcat cattgcggat tcctacatcg atcatacgtg ctaaaatcgt
     8761 aggaagtcct aattcaatca aaataaaacc aaatacgcat atgaaattga aaaacaagaa
     8821 tttcttataa cgcatggtat aatgccacat tagtttcact ttctcatctc cttttaatct
     8881 tttgttacat tttcattact aaaaatgata gcacaacgag cctattaata gaacacttct
     8941 ctcataaaaa agtcattatg attatagcgg gatgataaac attgattttt tctttcaatc
     9001 tagttgattt ctatacttat ttgctgtttt atgctagaat aacaattaat tgttgtatat
     9061 tttttataca gaaggtacgg ttgaaaaaat atccgaacag ataagcaaaa acgaggcttg
     9121 tctctttgac tcgaatccag tattctagtc aaagcatatg aaaaaccagg tggcttgtcc
     9181 cctcgttttg tgttgtctaa aaaaatcaac tagctaaatc actgctttac gcagataaag
     9241 gagacaaaac aatgatctat aaagtttatt atcaagaaac aaaagtacgc aaccctaaac
     9301 gggaagaaac aaaatctctt tacattgagg caaaaagcga tgtagacgct cgccaacgtg
     9361 tggaagaaaa taccccatac aatattgaat tcgttcaact tctagaagga agtcatctag
     9421 agtacgaaaa agaaaatgct gatttctcat tagtggagtt ttaatatgaa actttccatc
     9481 aaaaataacg agacaggcgt ctttgctatt ggtggattag gtgaaatcgg gaaaaatacg
     9541 tatggggtac aattccaaga tgaaatcatt ttgattgatg ccgggatcaa attcccggaa
     9601 gacgatttac ttggaattga ctatgtcatt cccgactaca gctacatcat acaaaactta
     9661 cacaaagtaa aagccttggt gattactcac gggcacgaag accacatcgg cggaataccc
     9721 tacttgctga aacaagcaaa tattcctatc tatgctggtc ctctcgcatt agcgttgatc
     9781 acaaacaaat tagatgagca tggcttgctt cgagatgcag agctccatga gatcaatgaa
     9841 gatacgatca tccgttttag aaagacttct gtcagctttt tcagaacgac ccatagtatc
     9901 ccagatgcat tagggatcgt ggtgaaaaca ccttctggaa atatcgtagc gactggagac
     9961 ttcaaattcg atttcactcc tgttggcgaa ccagcaaact tgcataaaat ggctcgtatc
    10021 ggggaagaag gagtcttgtg ccttttatct gatagtacaa atgctgagat tccaacattt
    10081 actaaatcag aaaaaacgat tgggcattct attttgaaga tcttttcaaa aatcgaagga
    10141 cgtatcattt ttgccagctt tgcttctaac atcttccgtt tgcagcaagc agcagatgcc
    10201 gctgtggcaa ctggtcgtaa aatcgctgtt ttcggacgaa gcatggaaaa tgccatcgtc
    10261 aacggtcaac gtttaggata tatcaaagtt ccagacggca catttgtaga tgcacacgaa
    10321 atcaatcgct tgcctgccaa tgaaacgatg atcctatgta caggttctca aggagaagcg
    10381 atggccgcgt tgagccgcat cgcgaatggt actcaccgtc aaatccaaat ccagccagac
    10441 gatacagtga tcttttcaag ttcacctatt cctggtaata caacaagtgt caatcatttg
    10501 atcaatttgc tgtctgaagc tggtgcagaa gtgattcacg gaaaaatcaa taacatccat
    10561 acttccggtc acggtggaca agaagaacaa aaattaatgc ttcgtttgat gaagccaaaa
    10621 tattttattc ctgtccacgg agaattccgc atgttgaaag tacatgcttc tctagcacaa
    10681 gatgtaggtg ttcctgcaga aaactgcttc atcatggaaa atggcgatgt tttagcgtta
    10741 actagcgatt ctgcgcgtcc tgctgggcat tttgtggctg gagatgtgta tatcgatggt
    10801 agcgggatcg gtgatattgg taatgtcgtt ttacgtgacc gtcacctgct ttcagaagaa
    10861 ggtcttgtat tagctgtcgc gacagtggat atcaagaaaa aagaaatctt ggctggtcca
    10921 gatattctct ctcgtggatt tgtctacatg cgagaatcag gagaaatgat caatgaagct
    10981 cagcgaatct tattccacgc tttaagagaa tgtttgaatg gacctgactg ttcagaatat
    11041 aaattgaaag aagcgatgac tggcgcgctt cagcctttct tattcgaacg tacagaaaga
    11101 catccaatga ttttgccaat ggtaatgaca ccagatgaag aataaataag aggttatgaa
    11161 atcagcacct tgtcctgaat ataagagaaa acaaatgaaa aacatgagaa gccatttttg
    11221 tttgttctta ttccttgata caaggtggct ctttcatagc ctcttaaatt tatttacttt
    11281 ttcatacaaa aaaatacctg ccacaagaga aattgtctct tgtggcaggt atttttgtta
    11341 ctcgctttgt tctctcgtaa aaattgatgt ctaccatcaa tttgatttac agataaaatt
    11401 aagcttttac aacgtttgca gcttgtgggc cgcggtctga ttcttcaacg tcgaaagtca
    11461 ctgcttgacc ttcttctaat gttttgaatc cgtcaccttg gatagctgag aagtgaacga
    11521 atacgtcgct tccatcttca cgtgagataa atccaaatcc tttttcagcg ttgaaccatt
    11581 ttactgttcc gttttccata ataatatcct cctcgtgcca aaagcactaa tttaaattgt
    11641 aacattgctc gtgtacgata gaggtgcaac agatgtttca acttctattc ccaaacaaaa
    11701 ttacaatata attgtacact tgcttcattt tttttgcaag aaagcaactt acttctcttc
    11761 catcattcct tcttctctca aactaaaata tcgtttgcgt ccgatgatca agtggtccag
    11821 tacagtgatc cccatcattt ctccacactt ttgtatcctt ttggtaaaat tcatatcctg
    11881 ctgcgatgga atttcatatt atcaagagta aattacaaaa gaatcattta aatcaatact
    11941 ttcactttga atacatgtat ttgaagtgga aggtacttat ttcaaaaata gtaaaacctg
    12001 tatcttaaat tacttaatag taacataaga tacaggctga ttttttattc attgttattt
    12061 atactaaagc acccgatagc tctgaaaaca atcacaaatt caacttttca aagccacagc
    12121 tttaagttat tttgtccaga caacccccat tgcccaaccc attttatgga attggcatcc
    12181 aggcaacaac ttttcatata aatcgtaata attttgttca gataggtact tatctgaagc
    12241 taaatgctca agccatgatt tagatgtgtt gtgattataa attctaatcg cattttttat
    12301 tccaaatttg aaacaatgcg gtaaaaactc ttggattgcc cctaatttat aaacataggt
    12361 aggcggtgtc tcgacttcag atacattatc aagaataact attcttcctt cctcattcaa
    12421 cagttccttc atttgctgta ttacgctggc tatatcatcc aaatgatgaa aggttgttcg
    12481 gcttacaata aaatcaaact tctcattaaa attaagttgt tctgcattca tattcagata
    12541 gaccgtattt gttagttgac gtttagattt ggcaagatcg agcatttgat tagaaatatc
    12601 aatccctacc acttcatcat aataacttgc taatttctcc actaacaaac ccgagccaca
    12661 tccgatatct aatgctctgc ctttctttgg agacatatta gacacaaaga atgaataatc
    12721 attcaaaagc tcattcacga aatcgtaatc ttctgcaacc ttatcaaact gtgattctat
    12781 tgtattcaaa aagatccccc attcctactt tatcgacatt ctttcattac ttaccacttt
    12841 agatgttttt tcgttgggga taaaacttcc ctttagacaa ttttatccaa agacaataca
    12901 acagtgcaac tttattaaag tcactgtcct ttatcgcagc ctttactttt tagtaaagac
    12961 agtggcttct cttatcaagt ttcaaaacat attattttga agaaaacgtc catctgaagt
    13021 gtcaagtgca aaattacata taaaggttta ttctaaaatg aaaagatgat acaatcatat
    13081 tcagttacat aaggaggttt caattatgtg caccagtatc gcagtagtag aaattacttt
    13141 atctcattca taatgaaaaa aatggaaagg agataaaagt atgggtactt tttctatatt
    13201 tgttattaat aaagttcgtt atcaaccaaa tcaaaattaa ttggttataa tgaacgctta
    13261 atgtcagttc attataacca gtaaggagaa ggttataatg aaccagaaaa accctaaaga
    13321 cacgcaaaat tttattactt ctaaaaagca tgtaaaagaa atattgaatc acacgaatat
    13381 cagtaaacaa gacaacgtaa tagaaatcgg atcaggaaaa ggacatttta ccaaagagct
    13441 agtcaaaatg agtcgatcag ttactgctat agaaattgat ggaggcttat gtcaagtgac
    13501 taaagaagcg gtaaacccct ctgagaatat aaaagtgatt caaacggata ttctaaaatt
    13561 ttccttccca aaacatataa actataagat atatggtaat attccttata acatcagtac
    13621 ggatattgtc aaaagaatta cctttgaaag tcaggctaaa tatagctatc ttatcgttga
    13681 gaagggattt gcgaaaagat tgcaaaatct gcaacgagct ttgggtttac tattaatggt
    13741 ggagatggat ataaaaatgc tcaaaaaagt accaccacta tattttcatc ctaagccaag
    13801 tgtagactct gtattgattg ttcttgaacg acatcaacca ttgatttcaa agaaggacta
    13861 caaaaagtat cgatcttttg tttataagtg ggtaaaccgt gaatatcgtg ttcttttcac
    13921 taaaaaccaa ttccgacagg ctttgaagca tgcaaatgtc actaatatta ataaactatc
    13981 gaaggaacaa tttctttcta ttttcaatag ttacaaattg tttcactaaa ttaaagtaat
    14041 aaagcgttct ctaatttcac aagaggacgc tttattcttc ccaaaaattg ttcaatattt
    14101 atcaataaat cagtagtttt aaaagtaagc acctgttatt gcaataaaat tagcctaatt
    14161 gagagaagtt tctatagaat ttttcatata cttaacgagt gctttcacct ttgaatatag
    14221 tccttcccac ttatcatcac actctccccg atagcctttt ctagctatat ccagtaaagt
    14281 tacatgctct ttaggtaaaa gaggtatagc ccattctgca gcgacatctt tcgaggtaat
    14341 ttcaccagta gtcactgttt gccacattcg agctagggtt aaaattacat tacgctcatc
    14401 accttttatc ccctcaatta gttctggcaa agaatcctta attgctcttc gaatatctgt
    14461 caaaggtacg gagacaagta tacttgaaga atcaggacca aatagagaaa tactattctt
    14521 tcttgcttgt gctaaaacaa tagccaaatc aggatcatag cttggttcct gaatttgtcc
    14581 attctcaaat tcacccctga gccactcacc gtatataaat tctctttttg gaggatattg
    14641 ccaagggaca acttcactcc tatttataac cgtaacttca agtggtctaa cagaatccgt
    14701 atttccaatc tttcctgata tagtcattag tctttctgtt agtttttttc gagttaattg
    14761 aggtaaacta tgattcacga cgactagaac atctacatcg ctgttaatgc gtaaaccacc
    14821 atttactgct gaaccaaata gatatactcc aactattgaa cttccaaata aatcttttac
    14881 gatttttaat gtttgaatcg cttgatttgg tatttttccg ttaatcaaat tgctcatgat
    14941 ttcacctcgt aactgattat gttcatataa agtttatatt gatactcaat ttacttaccc
    15001 tagattggac atatacctaa atgactgttc aataaagctg accgttagcg tttaagtaca
    15061 tcctttcaca atttgtctac agattaataa ttattcttta ttatacagat ctccatataa
    15121 tttttgaatt tggttctgta attttttatt ttctttttct aattccatta ctcttctttt
    15181 taaggtttta ataaggattt cctccgaacg agaacttttc ttgggttttg agactacatt
    15241 tgctgttatt tgacgctcac gaagggattc gattctttgc ctaatatcgt gttccttata
    15301 aagccatgat ttagaaacat tagcttcctt tgctattgaa ttaaaattaa taactttacc
    15361 ttcaatcgaa aatttagaaa tcgctttgtc tactttttcc cttgtctttt ttgatttctg
    15421 cttcgccaaa cgtacaattt ctgttgtatt tctaacttgt ttatccattg ataattaccc
    15481 cgtcaaactt ccaatgattt gttctaaacg ctctttaaca cggctattag tctctacttg
    15541 tctttgccat tgtttatcct tagctatggc taataactct tctgtacgct ctaactgttc
    15601 ttcgtgctgt ggtaagaatt gcttactggt acagaagtga gtgcaatcta agcatgcatt
    15661 cgcatgtgga caaccacctg ctactactgg caatctacaa taaccatttg gaagcacttg
    15721 tgcatttata tttttcttga accattgaag ctctacatca tcgacttcat tatcttcatc
    15781 tagatcaagc acatctccat tattggtaac cagtttttcc tgaaatttag taaattcatt
    15841 ttttagagtt tcatcaaaga tatgagcgta tctgcttgtc atttctgggc tttcatgccc
    15901 caaaaatttc tgcacaatat gctggggcat cccgttgtta atcattcttg ttcctactgt
    15961 atggcgaaag gcatgggcat ggaatctata aatctcacct gatttatcca ctatattttg
    16021 ctcataagct aatttattta actcacctct aaatgtttct tgttttaatg gcgatccatc
    16081 ttttcttgga aagaggtatt cactatctgg aaattcctct gaaactttat cttcccgaac
    16141 tttaataagt aaagctacct ctttagatat tggaactata tgctcctttt tcattttcca
    16201 ttgataatac tttaaaaaga aatctccatc tttgtcctct aatagacagc cttttttcaa
    16261 ggtgcacaat tcacttatcc tcattccaca ttcttgaaca atcatagtca tcgtagctat
    16321 atattcgggt aatttatcaa gatgactgtt caattgctct aggacgaatt catctataaa
    16381 gcgtggtttt gctcttggta ttttcggata gtcctcagaa taaattaata ttttggaagg
    16441 aacatcatcc cattctagcc taagaagggt actaaatagt ccttccaata tagagatcct
    16501 cccagttatt gtactaggtt ttattcccat catgtttagt tcacttaaat atgcttcaat
    16561 ttccactctc gttaattggt gtactctctg aacttgttta aatttcatgt ccagaaaatt
    16621 aaagaactct ttaagtcttt gggcaatatc acttacatag gaaaagctat ccatgttcaa
    16681 tctcaactta caatatcttt ttacaagttg tttaaaatat gtattccgaa accctttaaa
    16741 gttaattgta tattcatatt gtgttgggtt aaccttatca tctggcaaag gtaagttacg
    16801 tctatcccaa acgtctttat cccactcctc tccatcaaaa taaaagttct cataaaactc
    16861 cataaattgt tttagattag taacatagta ggaattagct tttacaggtg ttttttcttg
    16921 attagcagta atcttataat tagtagtggt aattctaaca ccccgttttg tcaaataagt
    16981 tctatactcc gtcattgctt tttcaatagg aacttcagta attgaagtaa tgctaggata
    17041 ctttaaatct aagaaatcta acattttatt aattactgtt ccttttctaa tccagacagt
    17101 ttttgcattc catattcc
//
LOCUS       DANJJW010000296        13213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.074, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000296 DANJJW010000000
VERSION     DANJJW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(204..2162)
                     /locus_tag="PWS82_003052"
     CDS             complement(204..2162)
                     /locus_tag="PWS82_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0908080.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PWS82_003053"
     CDS             complement(2159..3031)
                     /locus_tag="PWS82_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0908081.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PWS82_003054"
     CDS             complement(3373..4245)
                     /locus_tag="PWS82_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0908082.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PWS82_003055"
     CDS             4373..4942
                     /locus_tag="PWS82_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0908083.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PWS82_003056"
     CDS             5009..5746
                     /locus_tag="PWS82_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0908084.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PWS82_003057"
     CDS             5977..7245
                     /locus_tag="PWS82_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0908085.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PWS82_003058"
     CDS             7732..8196
                     /locus_tag="PWS82_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0908086.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PWS82_003059"
     CDS             8180..8809
                     /locus_tag="PWS82_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0908087.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWS82_003060"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWS82_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0908088.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PWS82_003061"
     CDS             10688..11134
                     /locus_tag="PWS82_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0908089.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PWS82_003062"
     CDS             complement(11333..11848)
                     /locus_tag="PWS82_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0908090.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PWS82_003063"
     CDS             11965..13167
                     /locus_tag="PWS82_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0908091.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DANJJW010000297         7926 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.118, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000297 DANJJW010000000
VERSION     DANJJW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(134..577)
                     /locus_tag="PWS82_003064"
     CDS             complement(134..577)
                     /locus_tag="PWS82_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL0908092.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PWS82_003065"
     CDS             complement(795..2135)
                     /locus_tag="PWS82_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0908093.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PWS82_003066"
     CDS             2404..3300
                     /locus_tag="PWS82_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0908094.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PWS82_003067"
     CDS             complement(3450..4391)
                     /locus_tag="PWS82_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0908095.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PWS82_003068"
     CDS             4517..5638
                     /locus_tag="PWS82_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0908096.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWS82_003069"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWS82_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0908097.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..>7926
                     /locus_tag="PWS82_003070"
     CDS             7465..>7926
                     /locus_tag="PWS82_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0908098.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFH"
BASE COUNT         2636 a         1488 c         1570 g         2232 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcat
//
LOCUS       DANJJW010000298         5613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.143, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000298 DANJJW010000000
VERSION     DANJJW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..1026)
                     /locus_tag="PWS82_003071"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="PWS82_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="PWS82_003072"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="PWS82_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0908099.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="PWS82_003073"
     CDS             1851..3005
                     /locus_tag="PWS82_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0908100.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="PWS82_003074"
     CDS             3220..4188
                     /locus_tag="PWS82_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0908101.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="PWS82_003075"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="PWS82_003075"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0908102.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="PWS82_003076"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="PWS82_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DANJJW010000299         1504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.231, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000299 DANJJW010000000
VERSION     DANJJW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            262..345
                     /locus_tag="PWS82_003077"
                     /pseudo
     CDS             262..345
                     /locus_tag="PWS82_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="PWS82_003078"
     CDS             394..477
                     /locus_tag="PWS82_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL0908103.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="PWS82_003079"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="PWS82_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0908104.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DANJJW010000300         1435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.237, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000300 DANJJW010000000
VERSION     DANJJW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            24..416
                     /locus_tag="PWS82_003080"
     CDS             24..416
                     /locus_tag="PWS82_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0908105.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1435
                     /gene="aac(6')-Ie"
                     /locus_tag="PWS82_003081"
     CDS             417..>1435
                     /gene="aac(6')-Ie"
                     /locus_tag="PWS82_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDL0908106.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETI"
BASE COUNT          589 a          116 c          238 g          492 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact attta
//
LOCUS       DANJJW010000301         1350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.239, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000301 DANJJW010000000
VERSION     DANJJW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            47..298
                     /locus_tag="PWS82_003082"
                     /pseudo
     CDS             47..298
                     /locus_tag="PWS82_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="PWS82_003083"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="PWS82_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0908107.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DANJJW010000302         1194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.245, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000302 DANJJW010000000
VERSION     DANJJW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="PWS82_003084"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="PWS82_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL0908108.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DANJJW010000303         1040 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.256, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000303 DANJJW010000000
VERSION     DANJJW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            94..969
                     /locus_tag="PWS82_003085"
                     /note="aph(2'')-Ia"
     CDS             94..969
                     /locus_tag="PWS82_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDL0908109.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          443 a           76 c          174 g          347 t
ORIGIN      
        1 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
       61 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
      121 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
      181 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
      241 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
      301 cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta taaagaaatt
      361 aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga acaaaatttg
      421 ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta tacagatatt
      481 agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt gttgcgtgaa
      541 actatttata atgatttaac tgatatagaa aaagattata tagaaagttt tatggaaaga
      601 ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga ttttagttgt
      661 aatcatctat tgttagatgg caataataga ttaactggaa taattgattt tggagattct
      721 ggaattatag atgaatattg tgattttata tacttacttg aagatagtga agaagaaata
      781 ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat tgagaaagca
      841 aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt ttatggaatt
      901 aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa aaggacttat
      961 aaagattgat tatataatat atgaaaagct attataaaag acggttctgt tgcaaagttt
     1021 taaatctact atcaaataag
//
LOCUS       DANJJW010000304          827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538676-rid17232843.guided.273, whole genome shotgun
            sequence.
ACCESSION   DANJJW010000304 DANJJW010000000
VERSION     DANJJW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538676
            Sequence Read Archive: ERR10688239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637975.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:46:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 26 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538676-rid17232843.guided.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            214..762
                     /gene="aac(6')"
                     /locus_tag="PWS82_003086"
     CDS             214..762
                     /gene="aac(6')"
                     /locus_tag="PWS82_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0908110.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          294 a          124 c          178 g          231 t
ORIGIN      
        1 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
       61 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
      121 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
      181 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
      241 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
      301 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
      361 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
      421 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
      481 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
      541 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
      601 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
      661 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
      721 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
      781 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
